MS020778 (gene) Bitter gourd (TR) v1

Overview
NameMS020778
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionHigh-affinity nitrate transporter 2.2
Locationscaffold487: 427937 .. 428120 (+)
RNA-Seq ExpressionMS020778
SyntenyMS020778
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AATACAGAGCACAAATCCACTAAGTTGAATATCTTCTCCATCGTTGCACCCACATGCGAGCGTTCCACGCCTCTCGTGGATTTCCTTTTGCACATGCTGTGTCTCCAGTTTTGCGGCGGCGCCGCTCCTCCCGGTGATCCATAACAACTTTAACCTCACCAACACGAAGATTGGCAACGTCGGA

mRNA sequence

AATACAGAGCACAAATCCACTAAGTTGAATATCTTCTCCATCGTTCCACATGCGAGCGTTCCACGCCTCTCGTGGATTTCCTTTTGCACATGCTGTGTCTCCAGTTTTGCGGCGGCGCCGCTCCTCCCGGTGATCCATAACAACTTTAACCTCACCAACACGAAGATTGGCAACGTCGGA

Coding sequence (CDS)

AATACAGAGCACAAATCCACTAAGTTGAATATCTTCTCCATCGTTCCACATGCGAGCGTTCCACGCCTCTCGTGGATTTCCTTTTGCACATGCTGTGTCTCCAGTTTTGCGGCGGCGCCGCTCCTCCCGGTGATCCATAACAACTTTAACCTCACCAACACGAAGATTGGCAACGTCGGA

Protein sequence

NTEHKSTKLNIFSIVPHASVPRLSWISFCTCCVSSFAAAPLLPVIHNNFNLTNTKIGNVG
Homology
BLAST of MS020778 vs. NCBI nr
Match: KAG6601518.1 (High affinity nitrate transporter 2.5, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032298.1 High affinity nitrate transporter 2.5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 85.5 bits (210), Expect = 1.7e-13
Identity = 42/61 (68.85%), Postives = 47/61 (77.05%), Query Frame = 0

Query: 1  NTEHKSTKLNIFSI-VPHASVPRLSWISFCTCCVSSFAAAPLLPVIHNNFNLTNTKIGNV 60
          ++EHKSTK NIFS+  PH     LSWISFC C VSSFAAAPLLPVI +N NLT+T IGN 
Sbjct: 20 DSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFVSSFAAAPLLPVIRDNLNLTDTDIGNA 79

BLAST of MS020778 vs. NCBI nr
Match: XP_023551949.1 (high affinity nitrate transporter 2.5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 85.5 bits (210), Expect = 1.7e-13
Identity = 42/61 (68.85%), Postives = 47/61 (77.05%), Query Frame = 0

Query: 1  NTEHKSTKLNIFSI-VPHASVPRLSWISFCTCCVSSFAAAPLLPVIHNNFNLTNTKIGNV 60
          ++EHKSTK NIFS+  PH     LSWISFC C VSSFAAAPLLPVI +N NLT+T IGN 
Sbjct: 20 DSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFVSSFAAAPLLPVIRDNLNLTDTDIGNA 79

BLAST of MS020778 vs. NCBI nr
Match: XP_022957579.1 (high affinity nitrate transporter 2.5 [Cucurbita moschata])

HSP 1 Score: 85.5 bits (210), Expect = 1.7e-13
Identity = 42/61 (68.85%), Postives = 47/61 (77.05%), Query Frame = 0

Query: 1  NTEHKSTKLNIFSI-VPHASVPRLSWISFCTCCVSSFAAAPLLPVIHNNFNLTNTKIGNV 60
          ++EHKSTK NIFS+  PH     LSWISFC C VSSFAAAPLLPVI +N NLT+T IGN 
Sbjct: 20 DSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFVSSFAAAPLLPVIRDNLNLTDTDIGNA 79

BLAST of MS020778 vs. NCBI nr
Match: XP_022993820.1 (high affinity nitrate transporter 2.5 [Cucurbita maxima])

HSP 1 Score: 85.5 bits (210), Expect = 1.7e-13
Identity = 42/61 (68.85%), Postives = 47/61 (77.05%), Query Frame = 0

Query: 1  NTEHKSTKLNIFSI-VPHASVPRLSWISFCTCCVSSFAAAPLLPVIHNNFNLTNTKIGNV 60
          ++EHKSTK NIFS+  PH     LSWISFC C VSSFAAAPLLPVI +N NLT+T IGN 
Sbjct: 20 DSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFVSSFAAAPLLPVIRDNLNLTDTDIGNA 79

BLAST of MS020778 vs. NCBI nr
Match: XP_038891297.1 (high affinity nitrate transporter 2.5-like [Benincasa hispida])

HSP 1 Score: 85.1 bits (209), Expect = 2.2e-13
Identity = 41/61 (67.21%), Postives = 47/61 (77.05%), Query Frame = 0

Query: 1  NTEHKSTKLNIFSI-VPHASVPRLSWISFCTCCVSSFAAAPLLPVIHNNFNLTNTKIGNV 60
          ++EHKSTK NIFS+  PH     LSWISFC C +SSFAAAPLLPVI +N NLT+T IGN 
Sbjct: 23 DSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAAPLLPVIRDNLNLTDTDIGNA 82

BLAST of MS020778 vs. ExPASy Swiss-Prot
Match: O82811 (High-affinity nitrate transporter 2.1 OS=Arabidopsis thaliana OX=3702 GN=NRT2.1 PE=1 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 4.4e-12
Identity = 35/61 (57.38%), Postives = 41/61 (67.21%), Query Frame = 0

Query: 1   NTEHKSTKLNIFSIV-PHASVPRLSWISFCTCCVSSFAAAPLLPVIHNNFNLTNTKIGNV 60
           +TEHK+T   +FS   PH     LSWISF TC VS+FAAAPL+P+I  N NLT   IGN 
Sbjct: 44  DTEHKATVFKLFSFAKPHMRTFHLSWISFSTCFVSTFAAAPLVPIIRENLNLTKQDIGNA 103

BLAST of MS020778 vs. ExPASy Swiss-Prot
Match: Q9LMZ9 (High-affinity nitrate transporter 2.2 OS=Arabidopsis thaliana OX=3702 GN=NRT2.2 PE=2 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 1.8e-10
Identity = 33/61 (54.10%), Postives = 39/61 (63.93%), Query Frame = 0

Query: 1   NTEHKSTKLNIFSIV-PHASVPRLSWISFCTCCVSSFAAAPLLPVIHNNFNLTNTKIGNV 60
           + E K+T   +FS   PH     LSWISF TC VS+FAAAPL+P+I  N NLT   IGN 
Sbjct: 42  DAEDKATVFKLFSFAKPHMRTFHLSWISFSTCFVSTFAAAPLIPIIRENLNLTKHDIGNA 101

BLAST of MS020778 vs. ExPASy Swiss-Prot
Match: P0DKG9 (High-affinity nitrate transporter 2.1 OS=Oryza sativa subsp. japonica OX=39947 GN=NRT2.1 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 3.1e-10
Identity = 31/61 (50.82%), Postives = 41/61 (67.21%), Query Frame = 0

Query: 1   NTEHKSTKLNIFSIV-PHASVPRLSWISFCTCCVSSFAAAPLLPVIHNNFNLTNTKIGNV 60
           ++EHK+  + + S   PH     LSWISF +C VS+FAAAPL+P+I +N NLT   IGN 
Sbjct: 45  DSEHKAKTIRLLSFANPHMRTFHLSWISFFSCFVSTFAAAPLVPIIRDNLNLTKADIGNA 104

BLAST of MS020778 vs. ExPASy Swiss-Prot
Match: P0DKH0 (High-affinity nitrate transporter 2.2 OS=Oryza sativa subsp. japonica OX=39947 GN=NRT2.2 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 3.1e-10
Identity = 31/61 (50.82%), Postives = 41/61 (67.21%), Query Frame = 0

Query: 1   NTEHKSTKLNIFSIV-PHASVPRLSWISFCTCCVSSFAAAPLLPVIHNNFNLTNTKIGNV 60
           ++EHK+  + + S   PH     LSWISF +C VS+FAAAPL+P+I +N NLT   IGN 
Sbjct: 45  DSEHKAKTIRLLSFANPHMRTFHLSWISFFSCFVSTFAAAPLVPIIRDNLNLTKADIGNA 104

BLAST of MS020778 vs. ExPASy Swiss-Prot
Match: Q9FJH8 (High affinity nitrate transporter 2.4 OS=Arabidopsis thaliana OX=3702 GN=NRT2.4 PE=2 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 4.1e-10
Identity = 30/61 (49.18%), Postives = 40/61 (65.57%), Query Frame = 0

Query: 1   NTEHKSTKLNIFSI-VPHASVPRLSWISFCTCCVSSFAAAPLLPVIHNNFNLTNTKIGNV 60
           +TEHK+    + S   PH     L+WISF TC +S+FAAAPL+P+I +N NLT   +GN 
Sbjct: 44  DTEHKAKVFKLLSFEAPHMRTFHLAWISFFTCFISTFAAAPLVPIIRDNLNLTRQDVGNA 103

BLAST of MS020778 vs. ExPASy TrEMBL
Match: A0A6J1JZK1 (high affinity nitrate transporter 2.5 OS=Cucurbita maxima OX=3661 GN=LOC111489712 PE=4 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 8.3e-14
Identity = 42/61 (68.85%), Postives = 47/61 (77.05%), Query Frame = 0

Query: 1  NTEHKSTKLNIFSI-VPHASVPRLSWISFCTCCVSSFAAAPLLPVIHNNFNLTNTKIGNV 60
          ++EHKSTK NIFS+  PH     LSWISFC C VSSFAAAPLLPVI +N NLT+T IGN 
Sbjct: 20 DSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFVSSFAAAPLLPVIRDNLNLTDTDIGNA 79

BLAST of MS020778 vs. ExPASy TrEMBL
Match: A0A6J1H2D0 (high affinity nitrate transporter 2.5 OS=Cucurbita moschata OX=3662 GN=LOC111458928 PE=4 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 8.3e-14
Identity = 42/61 (68.85%), Postives = 47/61 (77.05%), Query Frame = 0

Query: 1  NTEHKSTKLNIFSI-VPHASVPRLSWISFCTCCVSSFAAAPLLPVIHNNFNLTNTKIGNV 60
          ++EHKSTK NIFS+  PH     LSWISFC C VSSFAAAPLLPVI +N NLT+T IGN 
Sbjct: 20 DSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFVSSFAAAPLLPVIRDNLNLTDTDIGNA 79

BLAST of MS020778 vs. ExPASy TrEMBL
Match: A0A5A7SWP7 (High affinity nitrate transporter 2.5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001200 PE=4 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.1e-13
Identity = 41/61 (67.21%), Postives = 47/61 (77.05%), Query Frame = 0

Query: 1  NTEHKSTKLNIFSI-VPHASVPRLSWISFCTCCVSSFAAAPLLPVIHNNFNLTNTKIGNV 60
          ++EHKSTK NIFS+  PH     LSWISFC C +SSFAAAPLLPVI +N NLT+T IGN 
Sbjct: 21 DSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAAPLLPVIRDNLNLTDTDIGNA 80

BLAST of MS020778 vs. ExPASy TrEMBL
Match: A0A0A0KTM3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G599860 PE=4 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.1e-13
Identity = 41/61 (67.21%), Postives = 47/61 (77.05%), Query Frame = 0

Query: 1  NTEHKSTKLNIFSI-VPHASVPRLSWISFCTCCVSSFAAAPLLPVIHNNFNLTNTKIGNV 60
          ++EHKSTK NIFS+  PH     LSWISFC C +SSFAAAPLLPVI +N NLT+T IGN 
Sbjct: 22 DSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAAPLLPVIRDNLNLTDTDIGNA 81

BLAST of MS020778 vs. ExPASy TrEMBL
Match: S4VIV8 (High-affinity nitrate transporter NRT2.3 OS=Cucumis sativus OX=3659 GN=NRT2.3 PE=2 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.1e-13
Identity = 41/61 (67.21%), Postives = 47/61 (77.05%), Query Frame = 0

Query: 1  NTEHKSTKLNIFSI-VPHASVPRLSWISFCTCCVSSFAAAPLLPVIHNNFNLTNTKIGNV 60
          ++EHKSTK NIFS+  PH     LSWISFC C +SSFAAAPLLPVI +N NLT+T IGN 
Sbjct: 15 DSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAAPLLPVIRDNLNLTDTDIGNA 74

BLAST of MS020778 vs. TAIR 10
Match: AT1G08090.1 (nitrate transporter 2:1 )

HSP 1 Score: 71.2 bits (173), Expect = 3.1e-13
Identity = 35/61 (57.38%), Postives = 41/61 (67.21%), Query Frame = 0

Query: 1   NTEHKSTKLNIFSIV-PHASVPRLSWISFCTCCVSSFAAAPLLPVIHNNFNLTNTKIGNV 60
           +TEHK+T   +FS   PH     LSWISF TC VS+FAAAPL+P+I  N NLT   IGN 
Sbjct: 44  DTEHKATVFKLFSFAKPHMRTFHLSWISFSTCFVSTFAAAPLVPIIRENLNLTKQDIGNA 103

BLAST of MS020778 vs. TAIR 10
Match: AT1G08100.1 (nitrate transporter 2.2 )

HSP 1 Score: 65.9 bits (159), Expect = 1.3e-11
Identity = 33/61 (54.10%), Postives = 39/61 (63.93%), Query Frame = 0

Query: 1   NTEHKSTKLNIFSIV-PHASVPRLSWISFCTCCVSSFAAAPLLPVIHNNFNLTNTKIGNV 60
           + E K+T   +FS   PH     LSWISF TC VS+FAAAPL+P+I  N NLT   IGN 
Sbjct: 42  DAEDKATVFKLFSFAKPHMRTFHLSWISFSTCFVSTFAAAPLIPIIRENLNLTKHDIGNA 101

BLAST of MS020778 vs. TAIR 10
Match: AT5G60770.1 (nitrate transporter 2.4 )

HSP 1 Score: 64.7 bits (156), Expect = 2.9e-11
Identity = 30/61 (49.18%), Postives = 40/61 (65.57%), Query Frame = 0

Query: 1   NTEHKSTKLNIFSI-VPHASVPRLSWISFCTCCVSSFAAAPLLPVIHNNFNLTNTKIGNV 60
           +TEHK+    + S   PH     L+WISF TC +S+FAAAPL+P+I +N NLT   +GN 
Sbjct: 44  DTEHKAKVFKLLSFEAPHMRTFHLAWISFFTCFISTFAAAPLVPIIRDNLNLTRQDVGNA 103

BLAST of MS020778 vs. TAIR 10
Match: AT1G12940.1 (nitrate transporter2.5 )

HSP 1 Score: 63.5 bits (153), Expect = 6.5e-11
Identity = 32/61 (52.46%), Postives = 38/61 (62.30%), Query Frame = 0

Query: 1  NTEHKSTKLNIFSIV-PHASVPRLSWISFCTCCVSSFAAAPLLPVIHNNFNLTNTKIGNV 60
          + E+K+T   +FS+  PH     LSW  F  C VS+FAA PLLPVI  N NLT T IGN 
Sbjct: 26 DAENKATTFRLFSVAKPHMRAFHLSWFQFFCCFVSTFAAPPLLPVIRENLNLTATDIGNA 85

BLAST of MS020778 vs. TAIR 10
Match: AT5G60780.1 (nitrate transporter 2.3 )

HSP 1 Score: 62.0 bits (149), Expect = 1.9e-10
Identity = 29/61 (47.54%), Postives = 40/61 (65.57%), Query Frame = 0

Query: 1   NTEHKSTKLNIFSIV-PHASVPRLSWISFCTCCVSSFAAAPLLPVIHNNFNLTNTKIGNV 60
           ++EH++   N  S   PH     L W+SF TC +S+FAAAPL+P+I +N +LT T IGN 
Sbjct: 51  DSEHRAKVFNPLSFAKPHMRAFHLGWLSFFTCFISTFAAAPLVPIIRDNLDLTKTDIGNA 110

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6601518.11.7e-1368.85High affinity nitrate transporter 2.5, partial [Cucurbita argyrosperma subsp. so... [more]
XP_023551949.11.7e-1368.85high affinity nitrate transporter 2.5 [Cucurbita pepo subsp. pepo][more]
XP_022957579.11.7e-1368.85high affinity nitrate transporter 2.5 [Cucurbita moschata][more]
XP_022993820.11.7e-1368.85high affinity nitrate transporter 2.5 [Cucurbita maxima][more]
XP_038891297.12.2e-1367.21high affinity nitrate transporter 2.5-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
O828114.4e-1257.38High-affinity nitrate transporter 2.1 OS=Arabidopsis thaliana OX=3702 GN=NRT2.1 ... [more]
Q9LMZ91.8e-1054.10High-affinity nitrate transporter 2.2 OS=Arabidopsis thaliana OX=3702 GN=NRT2.2 ... [more]
P0DKG93.1e-1050.82High-affinity nitrate transporter 2.1 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
P0DKH03.1e-1050.82High-affinity nitrate transporter 2.2 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q9FJH84.1e-1049.18High affinity nitrate transporter 2.4 OS=Arabidopsis thaliana OX=3702 GN=NRT2.4 ... [more]
Match NameE-valueIdentityDescription
A0A6J1JZK18.3e-1468.85high affinity nitrate transporter 2.5 OS=Cucurbita maxima OX=3661 GN=LOC11148971... [more]
A0A6J1H2D08.3e-1468.85high affinity nitrate transporter 2.5 OS=Cucurbita moschata OX=3662 GN=LOC111458... [more]
A0A5A7SWP71.1e-1367.21High affinity nitrate transporter 2.5 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A0A0KTM31.1e-1367.21Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G599860 PE=4 SV=1[more]
S4VIV81.1e-1367.21High-affinity nitrate transporter NRT2.3 OS=Cucumis sativus OX=3659 GN=NRT2.3 PE... [more]
Match NameE-valueIdentityDescription
AT1G08090.13.1e-1357.38nitrate transporter 2:1 [more]
AT1G08100.11.3e-1154.10nitrate transporter 2.2 [more]
AT5G60770.12.9e-1149.18nitrate transporter 2.4 [more]
AT1G12940.16.5e-1152.46nitrate transporter2.5 [more]
AT5G60780.11.9e-1047.54nitrate transporter 2.3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR044772Nitrate transporterPANTHERPTHR23515HIGH-AFFINITY NITRATE TRANSPORTER 2.3coord: 1..60
NoneNo IPR availablePANTHERPTHR23515:SF28HIGH AFFINITY NITRATE TRANSPORTER-RELATEDcoord: 1..60

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS020778.1MS020778.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0015112 nitrate transmembrane transporter activity