MS020766 (gene) Bitter gourd (TR) v1

Overview
NameMS020766
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionU-box domain-containing protein 62-like
Locationscaffold487: 300168 .. 303845 (+)
RNA-Seq ExpressionMS020766
SyntenyMS020766
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCTCCGAAGACATGAATGGGCTCAGCTCCCAGCTCGTCTTCTCCGACGAAGCCATGCCCTTCAATTGCGCCAACACGCCGCAGCGCCGAGTCGGCGACCCGGGTCCCAAAACCCGCGAGCTCTGCGGTTTCATGGACGACAAGCTCTTCCCTGTGGACCGCGACCGGTTCTTCGCGCACCAGGGCGCGGATTTCCGCCGTAGTGTGTTCAGTGACAGCCGGAATTGGAATGGGAATGGGAGTGCGAGAAGTGGGAGTGGGAAAGGATCGGAGGAGGAAGACGATGATGATGATGATGATGAAGAAAATGAGGTTGATGATGGTGATGATGATGTTGAGGGACTCGTATCTATGGATGATGTCAATAAGTGCAATAAAATGGCCAACCCCAACAGCAGCAACAGTGATAGAAACGATCAGAGTAGTGGCGGCTTAAAAAATGGGACGAACAAATTGGGAAATGGGAAGCCTAAGCACCATTCTTCTTTTGGTAAGAAGTTTGTACTCTCTCAACTTTCATTACCCAAAAGGAAAATTTATAGAATGCTTGTTTGGATCTTCTGCTCCCAATTTGGAATGTTTTGTTTGACTTTTCTTATGGGTTTTTAGGTGGAACCCTGTTGGACAAGGATGGAATGGTTGGCCCTTCAGCCAGTAACGCAGGAGCAACGCAGAGTGAGAATCATCCTCAGGCAAGGTTGGGGAATTACCCTAATGCGGTTACAATTGCAGAACCTGACGGTGATGCTTATTATTCCCAATATTTGCAAGGCACTGAGGGGTCTGCTTCTGGCCAGAAGGATTTGGGTATGGAAAATGGATGTGGGTTTAATGGGAGAAGGGATGTTTCAATTTCGAGCGAGCCCGGAGAGTCTCTGAGAACAATTCTCGCAGACCCTGTGACGTAAGTTCTTAATGTTTCCATTGTGTTCCAATATATTTTGGTTGAGCTTGTAAACCATGTGGAAGTATGAACTTTCTACTTGCAATTTGTGATAATGATATGTTCTTACATTCTCTCGTTTCTTTCGGTCCACATTCTGCAATGAAAATTGTACTTTTTGACTTATTTCTTATCCCAATATCTTCTGATGATATATTTACTTAGGTTCGTTTTTCTGATTGGAAACAGGGGTGCTCTTATGGATGATGCAATGATATTACCGTGTGGGCATTCCTTTGGTAGTGGTGGAATACAACATGTTCTTAGAGTAGTGAGTTACTGTCAAGAACACTGCTCATCTATACTGTTAAAATTTTGTTTTCATTTATACCATTCATCAGTTTTCTTTTATCACGCTAAAACATAAGAGATGCATGAAACAGTTCAGTGTCGAGTATCCTTATGTTTCATTTCGATTGCAAGCATAGAAACTTCAACATATTGTAAAGTTGGCTTTTGATTTGAAACTTTACTAGGCCTTTGTAGCAACCCATGCTGATTTAGTTGCAGAACTTCATCAAGCTGTCCTGTATTGTAGTTGTAATTGGAAGAAGGGTCCAACTGAGACGTAGAAGTATCCTGGAGACTTGGTTGAGGTTCTTTGCAAAATCCATATCAAATACTAGCTATTAGTCAAGTGGGATTTGCATACTTTCTGTACTTTATTACGAGTGCCCTTTTTTCATGCCCGCTTGTTTCAGTGAGATTTATATGAGGATATGAGTGTATAGTTTGCAGGAATTGTAAAAAATATACCAAATTTGCTATAAAAATGAGTCTGCTACCTTATATGCTGCGGCACTGCTTCAACTCTTAATATTTTGACATGAGAGGGGTTTGCATCTACAAGTTCATCACTTTTCAATAATTGGACTCCATGTGTGGCACCATTGAACTTATGAACTTGTGCTTTTCAGTGCATATGTAAAACTTGGAGACGTGTCTCTTAGCTGAGGGAAATCAAGTAATTTGCCTAGATGACAAGATTTTAGTCTTAGTGAGTTTGTCGTGCATTTTCTTAATTTCAGTGATAGGTGCTCGCTTTCTGTTGCAGCATTTGTTTTACCCGACTAGTGCACCTTGATATGCATTGCTATTTGGCTGCTCTATTGATTTTAACAAGTTTATTATTTTGATGATCTTGGTTTCATTGTGTTTCTGTATTTCTCTTGATGTCTTCCCAGAAATCTTGTTACTCGTGTTCTCAGTCGGTTTCTGAAGACTCTATTGCTCAGAACTTATGTGAGTAAAATTTGTCTGCTCAAGTAATTTTTTCCATCTCTTTATTACCGTTTGTAGAAAACTGGACGTTTGAAGAATAGATTGATATAGTTTTATTTAAAGGTTACTTTCGAAATGGGGAACTTGTCTTGATCCTTTATTTCATGTTATGGTATTTTCATCTTCAGCTCTTCAAGCTGCAGTGCAAGCATTCCGCAGGGAAGAAGAAATACTGTTTTACCGCTCACCAAAAAGGAGAAGGGAGAGGTTTGAGCAGGTATTTCCTTTATATCTCGGACTTTGGCATCTTAAGCCTGATAGTGAGATACTAGAACCAAATGTGGCGGGTGGTAATTGGCATTCGGAGATTTCAGTTTCCTGCCTTCTTTCTTTCTAGTCTTCCCTATCACCAAGACGCTACATTTGGTTCACTTGTGATAAGTTCTACTTGTGCATTCATTTTTGTCACTCTTTCGCTTCAGAGTGCACAATGTTCTCATAGTGGAACTGCTTCCAAGTTTTCAATAATCATTCCGGTGTTTGCCAACGTAGGACAAGGGTGGTTATGGTGATTTGACTCTCATGGATACTCCAAGGGGTCGAGGTGTTCAGTTTCCTTTTGTCGTGACAGATCGGGTAATTATCAAGGTGAGCAGAAAACTGTACTCTCATTTAGTCCTATATTATCAAATACTGAAACTAATATCAACTAAAATGGATCAGGGGAACAAGAGGACACCACAGCGTTTTGTGGGACGTGAGGCTGTTGTTACAACACAGTGCTTGAATGGATGGTTTGTCATTCTTTTCTCTGATTCTCAATACTTTTGCCTCATTCTTGTCCAATCCCCGGAGAAATAAATTGTTTCCTGTTAATCAGTATGTTTAAATTCTTTCCATTCTATAGGGACAAGTGTTTGAAGTCATGGAACTTTTCGTACCTATTCTATTCGATAAGCGTTTGAAGCATGTCATGTAGTAGGTTAGAGGACAGATTTACAAATGAGTTAAATGATGAATACAAGATTAACAATAAAGACTAAATAGTTTTCTATACATAGTCTAGGGATTAGAACATGACATTTGAAAGACTTGGAAGGAATCAATTTAACCCGTGATGGCCGACTACCTAGAATTATATCCTTCGAGTTTCTTTTTGGTAATCAAATATAGTAGGGTTAGTCAATTGTCCTATAAAATTAGTCGAGATGTGTACAAATTGGAAGTATCACACCATAGCATTTGGAAGTAAAAGGAGAATAAAATATAACATTTGAAAGTTGGACCATAAATAAGTAAGAAAGTTCTAAGTTATAGAATAGGATTTAAACTATCTTTAGTGATCTAATATAACTTGGTCAACAATGTATCCACTTCTGTTACACTGCAGGTATGTGGTGAAAACACTAGACAATGCAGAGAGTGTAAAGTTGCAGTACCGCTCGCTTGCAAAGGTTCCAAACGATGCAGCATCAAAACCGATACCAAGGAAGATCGCGCCGAATTGGCTC

mRNA sequence

ATGTCCTCCGAAGACATGAATGGGCTCAGCTCCCAGCTCGTCTTCTCCGACGAAGCCATGCCCTTCAATTGCGCCAACACGCCGCAGCGCCGAGTCGGCGACCCGGGTCCCAAAACCCGCGAGCTCTGCGGTTTCATGGACGACAAGCTCTTCCCTGTGGACCGCGACCGGTTCTTCGCGCACCAGGGCGCGGATTTCCGCCGTAGTGTGTTCAGTGACAGCCGGAATTGGAATGGGAATGGGAGTGCGAGAAGTGGGAGTGGGAAAGGATCGGAGGAGGAAGACGATGATGATGATGATGATGAAGAAAATGAGGTTGATGATGGTGATGATGATGTTGAGGGACTCGTATCTATGGATGATGTCAATAAGTGCAATAAAATGGCCAACCCCAACAGCAGCAACAGTGATAGAAACGATCAGAGTAGTGGCGGCTTAAAAAATGGGACGAACAAATTGGGAAATGGGAAGCCTAAGCACCATTCTTCTTTTGGTGGAACCCTGTTGGACAAGGATGGAATGGTTGGCCCTTCAGCCAGTAACGCAGGAGCAACGCAGAGTGAGAATCATCCTCAGGCAAGGTTGGGGAATTACCCTAATGCGGTTACAATTGCAGAACCTGACGGTGATGCTTATTATTCCCAATATTTGCAAGGCACTGAGGGGTCTGCTTCTGGCCAGAAGGATTTGGGTATGGAAAATGGATGTGGGTTTAATGGGAGAAGGGATGTTTCAATTTCGAGCGAGCCCGGAGAGTCTCTGAGAACAATTCTCGCAGACCCTGTGACGGGTGCTCTTATGGATGATGCAATGATATTACCGTGTGGGCATTCCTTTGGTAGTGGTGGAATACAACATGTTCTTAGAGTAAAATCTTGTTACTCGTGTTCTCAGTCGGTTTCTGAAGACTCTATTGCTCAGAACTTATCTCTTCAAGCTGCAGTGCAAGCATTCCGCAGGGAAGAAGAAATACTGTTTTACCGCTCACCAAAAAGGAGAAGGGAGAGGTTTGAGCAGGACAAGGGTGGTTATGGTGATTTGACTCTCATGGATACTCCAAGGGGTCGAGGTGTTCAGTTTCCTTTTGTCGTGACAGATCGGGTAATTATCAAGGTGAGCAGAAAACTGTACTCTCATTTAGTCCTATATTATCAAATACTGAAACTAATATCAACTAAAATGGATCAGGGGAACAAGAGGACACCACAGCGTTTTGTGGGACGTGAGGCTGTTGTTACAACACAGTGCTTGAATGGATGGTATGTGGTGAAAACACTAGACAATGCAGAGAGTGTAAAGTTGCAGTACCGCTCGCTTGCAAAGGTTCCAAACGATGCAGCATCAAAACCGATACCAAGGAAGATCGCGCCGAATTGGCTC

Coding sequence (CDS)

ATGTCCTCCGAAGACATGAATGGGCTCAGCTCCCAGCTCGTCTTCTCCGACGAAGCCATGCCCTTCAATTGCGCCAACACGCCGCAGCGCCGAGTCGGCGACCCGGGTCCCAAAACCCGCGAGCTCTGCGGTTTCATGGACGACAAGCTCTTCCCTGTGGACCGCGACCGGTTCTTCGCGCACCAGGGCGCGGATTTCCGCCGTAGTGTGTTCAGTGACAGCCGGAATTGGAATGGGAATGGGAGTGCGAGAAGTGGGAGTGGGAAAGGATCGGAGGAGGAAGACGATGATGATGATGATGATGAAGAAAATGAGGTTGATGATGGTGATGATGATGTTGAGGGACTCGTATCTATGGATGATGTCAATAAGTGCAATAAAATGGCCAACCCCAACAGCAGCAACAGTGATAGAAACGATCAGAGTAGTGGCGGCTTAAAAAATGGGACGAACAAATTGGGAAATGGGAAGCCTAAGCACCATTCTTCTTTTGGTGGAACCCTGTTGGACAAGGATGGAATGGTTGGCCCTTCAGCCAGTAACGCAGGAGCAACGCAGAGTGAGAATCATCCTCAGGCAAGGTTGGGGAATTACCCTAATGCGGTTACAATTGCAGAACCTGACGGTGATGCTTATTATTCCCAATATTTGCAAGGCACTGAGGGGTCTGCTTCTGGCCAGAAGGATTTGGGTATGGAAAATGGATGTGGGTTTAATGGGAGAAGGGATGTTTCAATTTCGAGCGAGCCCGGAGAGTCTCTGAGAACAATTCTCGCAGACCCTGTGACGGGTGCTCTTATGGATGATGCAATGATATTACCGTGTGGGCATTCCTTTGGTAGTGGTGGAATACAACATGTTCTTAGAGTAAAATCTTGTTACTCGTGTTCTCAGTCGGTTTCTGAAGACTCTATTGCTCAGAACTTATCTCTTCAAGCTGCAGTGCAAGCATTCCGCAGGGAAGAAGAAATACTGTTTTACCGCTCACCAAAAAGGAGAAGGGAGAGGTTTGAGCAGGACAAGGGTGGTTATGGTGATTTGACTCTCATGGATACTCCAAGGGGTCGAGGTGTTCAGTTTCCTTTTGTCGTGACAGATCGGGTAATTATCAAGGTGAGCAGAAAACTGTACTCTCATTTAGTCCTATATTATCAAATACTGAAACTAATATCAACTAAAATGGATCAGGGGAACAAGAGGACACCACAGCGTTTTGTGGGACGTGAGGCTGTTGTTACAACACAGTGCTTGAATGGATGGTATGTGGTGAAAACACTAGACAATGCAGAGAGTGTAAAGTTGCAGTACCGCTCGCTTGCAAAGGTTCCAAACGATGCAGCATCAAAACCGATACCAAGGAAGATCGCGCCGAATTGGCTC

Protein sequence

MSSEDMNGLSSQLVFSDEAMPFNCANTPQRRVGDPGPKTRELCGFMDDKLFPVDRDRFFAHQGADFRRSVFSDSRNWNGNGSARSGSGKGSEEEDDDDDDDEENEVDDGDDDVEGLVSMDDVNKCNKMANPNSSNSDRNDQSSGGLKNGTNKLGNGKPKHHSSFGGTLLDKDGMVGPSASNAGATQSENHPQARLGNYPNAVTIAEPDGDAYYSQYLQGTEGSASGQKDLGMENGCGFNGRRDVSISSEPGESLRTILADPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCSQSVSEDSIAQNLSLQAAVQAFRREEEILFYRSPKRRRERFEQDKGGYGDLTLMDTPRGRGVQFPFVVTDRVIIKVSRKLYSHLVLYYQILKLISTKMDQGNKRTPQRFVGREAVVTTQCLNGWYVVKTLDNAESVKLQYRSLAKVPNDAASKPIPRKIAPNWL
Homology
BLAST of MS020766 vs. NCBI nr
Match: XP_022146864.1 (U-box domain-containing protein 62 isoform X1 [Momordica charantia])

HSP 1 Score: 832.4 bits (2149), Expect = 1.9e-237
Identity = 433/461 (93.93%), Postives = 433/461 (93.93%), Query Frame = 0

Query: 1   MSSEDMNGLSSQLVFSDEAMPFNCANTPQRRVGDPGPKTRELCGFMDDKLFPVDRDRFFA 60
           MSSEDMNGLSSQLVFSDEAMPFNCANTPQRRVGDPGPKTRELCGFMDDKLFPVDRDRFFA
Sbjct: 1   MSSEDMNGLSSQLVFSDEAMPFNCANTPQRRVGDPGPKTRELCGFMDDKLFPVDRDRFFA 60

Query: 61  HQGADFRRSVFSDSRNWNGNGSARSGSGKGSEEE-DDDDDDDEENEVDDGDDDVEGLVSM 120
           HQGADFRRSVFSDSRNWNGNGSARSGSGKGSEEE DDDDDDDEENEVDDGDDDVEGLVSM
Sbjct: 61  HQGADFRRSVFSDSRNWNGNGSARSGSGKGSEEEDDDDDDDDEENEVDDGDDDVEGLVSM 120

Query: 121 DDVNKCNKMANPNSSNSDRNDQSSGGLKNGTNKLGNGKPKHHSSFGGTLLDKDGMVGPSA 180
           DDVNKCNKMANPNSSNSDRNDQSSGGLKNGTNKLGNGKPKHHSSFGGTLL KDGMVGPSA
Sbjct: 121 DDVNKCNKMANPNSSNSDRNDQSSGGLKNGTNKLGNGKPKHHSSFGGTLLVKDGMVGPSA 180

Query: 181 SNAGATQSENHPQARLGNYPNAVTIAEPDGDAYYSQYLQGTEGSASGQKDLGMENGCGFN 240
           SNAGATQSENHPQARLGNYPNAVTIAEPDGDAYYSQYLQGTEGSASGQKDLGMENGCGFN
Sbjct: 181 SNAGATQSENHPQARLGNYPNAVTIAEPDGDAYYSQYLQGTEGSASGQKDLGMENGCGFN 240

Query: 241 GRRDVSISSEPGESLRTILADPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCSQS 300
           GRRD SISSEPGESLRTILADPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCSQS
Sbjct: 241 GRRDASISSEPGESLRTILADPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCSQS 300

Query: 301 VSEDSIAQNLSLQAAVQAFRREEEILFYRSPKRRRERFEQDKGGYGDLTLMDTPRGRGVQ 360
           VSEDSIAQNLSLQAAVQAFRREEEILFYRSPKRRRERFEQDKGGYGDLTLMDTPRGRGVQ
Sbjct: 301 VSEDSIAQNLSLQAAVQAFRREEEILFYRSPKRRRERFEQDKGGYGDLTLMDTPRGRGVQ 360

Query: 361 FPFVVTDRVIIKVSRKLYSHLVLYYQILKLISTKMDQGNKRTPQRFVGREAVVTTQCLNG 420
           FPFVVTDRVIIK                         GNKRTPQRFVGREAVVTTQCLNG
Sbjct: 361 FPFVVTDRVIIK-------------------------GNKRTPQRFVGREAVVTTQCLNG 420

Query: 421 WYVVKTLDNAESVKLQYRSLAKVPNDAASKPIPRKIAPNWL 461
           WYVVKTLDNAESVKLQYRSLAKVPNDAASKPIPRKIAPNWL
Sbjct: 421 WYVVKTLDNAESVKLQYRSLAKVPNDAASKPIPRKIAPNWL 436

BLAST of MS020766 vs. NCBI nr
Match: XP_022146865.1 (U-box domain-containing protein 62 isoform X2 [Momordica charantia])

HSP 1 Score: 823.5 bits (2126), Expect = 8.8e-235
Identity = 431/461 (93.49%), Postives = 431/461 (93.49%), Query Frame = 0

Query: 1   MSSEDMNGLSSQLVFSDEAMPFNCANTPQRRVGDPGPKTRELCGFMDDKLFPVDRDRFFA 60
           MSSEDMNGLSSQLVFSDEAMPFNCANTPQRRVGDPGPKTRELCGFMDDKLFPVDRDRFFA
Sbjct: 1   MSSEDMNGLSSQLVFSDEAMPFNCANTPQRRVGDPGPKTRELCGFMDDKLFPVDRDRFFA 60

Query: 61  HQGADFRRSVFSDSRNWNGNGSARSGSGKGSEEE-DDDDDDDEENEVDDGDDDVEGLVSM 120
           HQGADFRRSVFSDSRNW  NGSARSGSGKGSEEE DDDDDDDEENEVDDGDDDVEGLVSM
Sbjct: 61  HQGADFRRSVFSDSRNW--NGSARSGSGKGSEEEDDDDDDDDEENEVDDGDDDVEGLVSM 120

Query: 121 DDVNKCNKMANPNSSNSDRNDQSSGGLKNGTNKLGNGKPKHHSSFGGTLLDKDGMVGPSA 180
           DDVNKCNKMANPNSSNSDRNDQSSGGLKNGTNKLGNGKPKHHSSFGGTLL KDGMVGPSA
Sbjct: 121 DDVNKCNKMANPNSSNSDRNDQSSGGLKNGTNKLGNGKPKHHSSFGGTLLVKDGMVGPSA 180

Query: 181 SNAGATQSENHPQARLGNYPNAVTIAEPDGDAYYSQYLQGTEGSASGQKDLGMENGCGFN 240
           SNAGATQSENHPQARLGNYPNAVTIAEPDGDAYYSQYLQGTEGSASGQKDLGMENGCGFN
Sbjct: 181 SNAGATQSENHPQARLGNYPNAVTIAEPDGDAYYSQYLQGTEGSASGQKDLGMENGCGFN 240

Query: 241 GRRDVSISSEPGESLRTILADPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCSQS 300
           GRRD SISSEPGESLRTILADPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCSQS
Sbjct: 241 GRRDASISSEPGESLRTILADPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCSQS 300

Query: 301 VSEDSIAQNLSLQAAVQAFRREEEILFYRSPKRRRERFEQDKGGYGDLTLMDTPRGRGVQ 360
           VSEDSIAQNLSLQAAVQAFRREEEILFYRSPKRRRERFEQDKGGYGDLTLMDTPRGRGVQ
Sbjct: 301 VSEDSIAQNLSLQAAVQAFRREEEILFYRSPKRRRERFEQDKGGYGDLTLMDTPRGRGVQ 360

Query: 361 FPFVVTDRVIIKVSRKLYSHLVLYYQILKLISTKMDQGNKRTPQRFVGREAVVTTQCLNG 420
           FPFVVTDRVIIK                         GNKRTPQRFVGREAVVTTQCLNG
Sbjct: 361 FPFVVTDRVIIK-------------------------GNKRTPQRFVGREAVVTTQCLNG 420

Query: 421 WYVVKTLDNAESVKLQYRSLAKVPNDAASKPIPRKIAPNWL 461
           WYVVKTLDNAESVKLQYRSLAKVPNDAASKPIPRKIAPNWL
Sbjct: 421 WYVVKTLDNAESVKLQYRSLAKVPNDAASKPIPRKIAPNWL 434

BLAST of MS020766 vs. NCBI nr
Match: XP_038892965.1 (U-box domain-containing protein 62-like [Benincasa hispida])

HSP 1 Score: 695.3 bits (1793), Expect = 3.6e-196
Identity = 372/461 (80.69%), Postives = 393/461 (85.25%), Query Frame = 0

Query: 1   MSSEDMNGLSSQLVFSDEAMPFNCANTPQRRVGDPGPKTRELCGFMDDKLFP-VDRDRFF 60
           MSSED+NGL+S LVF DEA+PFNC+ TPQRRV DP PKTRELCGFMDDKLFP VDRDRFF
Sbjct: 1   MSSEDINGLNSHLVFQDEALPFNCSTTPQRRVPDPPPKTRELCGFMDDKLFPVVDRDRFF 60

Query: 61  AHQGADFRRSVFSDSRNWNGNGSARSGSGKGSEEEDDDDDDDEENEVDDGDDDVEGLVSM 120
           +HQG DFRRSVF  +RNWNGN +AR  SGKGS+EEDDDDDDDE+ EV+DGDDDVEGLVS+
Sbjct: 61  SHQGEDFRRSVFGHTRNWNGNETAR--SGKGSDEEDDDDDDDED-EVNDGDDDVEGLVSL 120

Query: 121 DDVNKCNKMANPNSSNSDRNDQSSGGLKNGTNKLGNGKPKHHSSFGGTLLDKDGMVGPSA 180
           DDVNKC+K ANPN SN+DR+DQSSGGLKNGT KLGNGK KHHSSFGGTL+ KDG+VG S 
Sbjct: 121 DDVNKCSKTANPNDSNNDRSDQSSGGLKNGTIKLGNGKLKHHSSFGGTLVVKDGLVGSSG 180

Query: 181 SNAGATQSENHPQARLGNYPNAVTIAEPDGDAYYSQYLQGTEGSASGQKDLGMENGCGFN 240
           ++ GATQSENHPQ RLGNYPNAVTIAE +GDAYYSQYLQ TEGSASGQKDL MENGCGF 
Sbjct: 181 NDTGATQSENHPQGRLGNYPNAVTIAEHEGDAYYSQYLQSTEGSASGQKDLAMENGCGF- 240

Query: 241 GRRDVSISSEPGESLRTILADPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCSQS 300
           GRRDVS S EPGESLR IL DPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCSQS
Sbjct: 241 GRRDVSFSGEPGESLRAILTDPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCSQS 300

Query: 301 VSEDSIAQNLSLQAAVQAFRREEEILFYRSPKRRRERFEQDKGGYGDLTLMDTPRGRGVQ 360
           VSEDSIAQNLSLQAAVQAFRREEE+LFYRSPKRRRERFEQ      DLT MDTPRGRGVQ
Sbjct: 301 VSEDSIAQNLSLQAAVQAFRREEELLFYRSPKRRRERFEQ------DLTFMDTPRGRGVQ 360

Query: 361 FPFVVTDRVIIKVSRKLYSHLVLYYQILKLISTKMDQGNKRTPQRFVGREAVVTTQCLNG 420
           FPF VTDRVIIK                         GNKRTPQRFVGREAVVTTQCLNG
Sbjct: 361 FPFTVTDRVIIK-------------------------GNKRTPQRFVGREAVVTTQCLNG 420

Query: 421 WYVVKTLDNAESVKLQYRSLAKVPNDAASKPIPRKIAPNWL 461
           WYVVKTLDNAESVKLQYRSLAKVP+DA SK I RK  PNWL
Sbjct: 421 WYVVKTLDNAESVKLQYRSLAKVPDDATSKTITRKFTPNWL 426

BLAST of MS020766 vs. NCBI nr
Match: XP_022942528.1 (U-box domain-containing protein 62 isoform X1 [Cucurbita moschata] >KAG6600875.1 U-box domain-containing protein 62, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031509.1 U-box domain-containing protein 62 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 680.2 bits (1754), Expect = 1.2e-191
Identity = 367/463 (79.27%), Postives = 386/463 (83.37%), Query Frame = 0

Query: 1   MSSEDMNGLSSQLVFSDEAMPFNCANTPQRRVGDPGPKTRELCGFMDDKLFPVDRDRFFA 60
           MSSEDMNGL+S LVF ++AMPFNCA T QRRV DP PKTRELCGFMDDKL PVDRDRFF 
Sbjct: 1   MSSEDMNGLNSHLVFQEDAMPFNCATTSQRRVADPAPKTRELCGFMDDKLLPVDRDRFFV 60

Query: 61  HQGADFRRSVFSDSRNWNGNGSARSGSGKGSEEEDDDD-DDDEENEVDDGDDD--VEGLV 120
           HQG DFRRSVF  SRNWNGN SAR+GSGKGS+EEDDDD DDD+E+EVDDGDDD  VEGL 
Sbjct: 61  HQGEDFRRSVFGHSRNWNGNESARTGSGKGSDEEDDDDEDDDDEDEVDDGDDDVVVEGLA 120

Query: 121 SMDDVNKCNKMANPNSSNSDRNDQSSGGLKNGTNKLGNGKPKHHSSFGGTLLDKDGMVGP 180
           S+DDVNKC K ANPN +N D +DQSS GLKNGTNKLGNGK KHHSSFGGTLL KDG+VG 
Sbjct: 121 SLDDVNKCTKTANPNITNKDISDQSSDGLKNGTNKLGNGKLKHHSSFGGTLLVKDGLVGS 180

Query: 181 SASNAGATQSENHPQARLGNYPNAVTIAEPDGDAYYSQYLQGTEGSASGQKDLGMENGCG 240
           S  N GAT+          N PNAVTIAEP+ +AYYSQYLQGTEGS SGQKDL +ENGCG
Sbjct: 181 SGCNTGATR----------NDPNAVTIAEPE-NAYYSQYLQGTEGSTSGQKDLAIENGCG 240

Query: 241 FNGRRDVSISSEPGESLRTILADPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCS 300
           F GRRDVS SSEP ESLR IL DPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCS
Sbjct: 241 F-GRRDVSFSSEPEESLRAILTDPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCS 300

Query: 301 QSVSEDSIAQNLSLQAAVQAFRREEEILFYRSPKRRRERFEQDKGGYGDLTLMDTPRGRG 360
           QSVSEDSIAQNLSLQAAVQAFRREEE+LFYRSPKRRRERFEQDKGGYGD TLMDTPRGRG
Sbjct: 301 QSVSEDSIAQNLSLQAAVQAFRREEELLFYRSPKRRRERFEQDKGGYGDSTLMDTPRGRG 360

Query: 361 VQFPFVVTDRVIIKVSRKLYSHLVLYYQILKLISTKMDQGNKRTPQRFVGREAVVTTQCL 420
           VQFPF VTDRVIIK                         GNKRTPQRFVGREAVVT QCL
Sbjct: 361 VQFPFAVTDRVIIK-------------------------GNKRTPQRFVGREAVVTAQCL 420

Query: 421 NGWYVVKTLDNAESVKLQYRSLAKVPNDAASKPIPRKIAPNWL 461
           NGWYVVKTLDNAESVKLQYRSLA+VPNDAASK I +K+APNWL
Sbjct: 421 NGWYVVKTLDNAESVKLQYRSLARVPNDAASKTITKKVAPNWL 426

BLAST of MS020766 vs. NCBI nr
Match: XP_004147826.3 (U-box domain-containing protein 62 isoform X1 [Cucumis sativus])

HSP 1 Score: 680.2 bits (1754), Expect = 1.2e-191
Identity = 362/461 (78.52%), Postives = 391/461 (84.82%), Query Frame = 0

Query: 1   MSSEDMNGLSSQLVFSDEAMPFNCANTPQRRVGDPGPKTRELCGFMDDKLFP-VDRDRFF 60
           MSSED+NGL S LVF +E++PFNC++TP RR+ DP PKTRELCGFMDDKLFP VDRDRFF
Sbjct: 1   MSSEDINGLDSHLVFQNESLPFNCSSTPHRRLPDPPPKTRELCGFMDDKLFPIVDRDRFF 60

Query: 61  AHQGADFRRSVFSDSRNWNGNGSARSGSGKGSEEEDDDDDDDEENEVDDGDDDVEGLVSM 120
           + QG DFRR+VF  +RNW  NGSAR+GSGKGS+EEDDDDDDD+E+EVDDGDDDVEGLVS+
Sbjct: 61  SQQGEDFRRNVFGHNRNW--NGSARTGSGKGSDEEDDDDDDDDEDEVDDGDDDVEGLVSL 120

Query: 121 DDVNKCNKMANPNSSNSDRNDQSSGGLKNGTNKLGNGKPKHHSSFGGTLLDKDGMVGPSA 180
           DDVNKC+K ANP  SN+DR+DQSS GLKNG NKLGNGK K HSSF GTLL KD  VG S 
Sbjct: 121 DDVNKCSKTANPIDSNNDRSDQSSEGLKNGINKLGNGKLKQHSSFCGTLLVKDAFVGSS- 180

Query: 181 SNAGATQSENHPQARLGNYPNAVTIAEPDGDAYYSQYLQGTEGSASGQKDLGMENGCGFN 240
              GATQSEN+PQ RLGNYPNAVT+AEPD +AYYSQYLQ  EGSASGQK+L MENGCGF 
Sbjct: 181 ---GATQSENNPQGRLGNYPNAVTVAEPDSEAYYSQYLQSNEGSASGQKNLSMENGCGF- 240

Query: 241 GRRDVSISSEPGESLRTILADPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCSQS 300
           GRRDVS S EPGESLR+IL DPVTGALM+DAMILPCGHSFGSGGIQHVL+V+SCYSCSQS
Sbjct: 241 GRRDVSFSGEPGESLRSILTDPVTGALMNDAMILPCGHSFGSGGIQHVLKVRSCYSCSQS 300

Query: 301 VSEDSIAQNLSLQAAVQAFRREEEILFYRSPKRRRERFEQDKGGYGDLTLMDTPRGRGVQ 360
           VSEDSIAQNLSLQAAVQAF REEE+LF+RSPKRRRERFEQDKGGYGDLTLMDT RGRGVQ
Sbjct: 301 VSEDSIAQNLSLQAAVQAFCREEELLFHRSPKRRRERFEQDKGGYGDLTLMDTQRGRGVQ 360

Query: 361 FPFVVTDRVIIKVSRKLYSHLVLYYQILKLISTKMDQGNKRTPQRFVGREAVVTTQCLNG 420
           FPF VTDRVIIK                         GNKRTPQRFVGREAVVTTQCLNG
Sbjct: 361 FPFAVTDRVIIK-------------------------GNKRTPQRFVGREAVVTTQCLNG 420

Query: 421 WYVVKTLDNAESVKLQYRSLAKVPNDAASKPIPRKIAPNWL 461
           WYVVKTLDNAESVKLQYRSLAKVPNDA +K I RKI PNWL
Sbjct: 421 WYVVKTLDNAESVKLQYRSLAKVPNDATTKTITRKITPNWL 429

BLAST of MS020766 vs. ExPASy Swiss-Prot
Match: Q6DBN5 (U-box domain-containing protein 62 OS=Arabidopsis thaliana OX=3702 GN=PUB62 PE=2 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 2.5e-67
Identity = 141/264 (53.41%), Postives = 170/264 (64.39%), Query Frame = 0

Query: 201 AVTIAEPDGDAYYSQYLQGTEG---SASGQKDLGMENGCGFNGRRDVSISSEPG-ESLRT 260
           AV +   D  AYYSQ+ +  E    S + +  +  +NGC F+GRRD+S SS    ESLRT
Sbjct: 125 AVIVVGQDNAAYYSQHFKTVEASFVSRNEESSIAADNGCDFSGRRDLSSSSSNSIESLRT 184

Query: 261 ILADPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCSQSVSEDSIAQNLSLQAAVQ 320
           IL+DP TG+LM DAMILPCGH+FG+GGI+ V ++K+C +CSQ VSEDSI  NL+L+ AVQ
Sbjct: 185 ILSDPTTGSLMADAMILPCGHTFGAGGIEQVKQMKACCTCSQPVSEDSITPNLTLRVAVQ 244

Query: 321 AFRREEEILFYRSPKRRRERFEQDKGGYGDLTLMDTPRGRGVQFPFVVTDRVIIKVSRKL 380
           AF REE        KR+RE F+Q++  +G        R +   FPF V DRVIIK     
Sbjct: 245 AFCREENSQSNHPSKRKREGFDQERRAFGVTNHSGRSRNKSNHFPFAVADRVIIK----- 304

Query: 381 YSHLVLYYQILKLISTKMDQGNKRTPQRFVGREAVVTTQCLNGWYVVKTLDNAESVKLQY 440
                               GNKRTP RFVGREAVVTTQCLNGWYVVKTLDNAESVKLQY
Sbjct: 305 --------------------GNKRTPPRFVGREAVVTTQCLNGWYVVKTLDNAESVKLQY 363

Query: 441 RSLAKVPNDAASKPIPRKIAPNWL 461
           RSLAK P D ++K  P K+  NWL
Sbjct: 365 RSLAKAPEDPSAKATPNKMVSNWL 363

BLAST of MS020766 vs. ExPASy Swiss-Prot
Match: Q58FY4 (U-box domain-containing protein 63 OS=Arabidopsis thaliana OX=3702 GN=PUB63 PE=2 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 3.4e-48
Identity = 114/261 (43.68%), Postives = 159/261 (60.92%), Query Frame = 0

Query: 202 VTIAEPDGDAYYS-QYLQGTEGSASGQKDLGMENGCGFNGRRDVSISSEPGESLRTILAD 261
           V I +PDG+   S +  Q     AS +K+            R  + S    ESL+ IL+D
Sbjct: 161 VRITKPDGNVSNSHRNTQQKRDFASVEKE------------RVTTTSVSSWESLKAILSD 220

Query: 262 PVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCSQSVSEDSIAQNLSLQAAVQAFRR 321
           PVTGALM+DA ILPCGHSFG+GG++ V ++K+C++CSQ   E S   NLSL+  V AFR+
Sbjct: 221 PVTGALMNDATILPCGHSFGAGGLKEVKKMKACFTCSQPTLEGSEKPNLSLRIVVHAFRQ 280

Query: 322 EEEILFYRSPKRRRERFEQDKGGYGDLTLMDTPR-GRGVQFPFVVTDRVIIKVSRKLYSH 381
           EE+     + KRR+ER +Q K  +    + +TP+  RG+QFPF + D +II         
Sbjct: 281 EEDSDHIHTLKRRKERSDQ-KRSFCIPNITETPKSSRGIQFPFSIGDHIII--------- 340

Query: 382 LVLYYQILKLISTKMDQGNKRTPQRFVGREAVVTTQCLNGWYVVKTLDNAESVKLQYRSL 441
                           +GNKRTP RFVGR AV+ TQCLNGWYVVKT+DN+ES+KLQ+ SL
Sbjct: 341 ----------------EGNKRTPPRFVGRIAVIMTQCLNGWYVVKTVDNSESIKLQHCSL 383

Query: 442 AKVPNDAASKPIPRKIAPNWL 461
           AK+ +++++K    ++ P+WL
Sbjct: 401 AKISDNSSTKVTVAEMPPSWL 383

BLAST of MS020766 vs. ExPASy TrEMBL
Match: A0A6J1CZA9 (U-box domain-containing protein 62 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111015962 PE=4 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 9.1e-238
Identity = 433/461 (93.93%), Postives = 433/461 (93.93%), Query Frame = 0

Query: 1   MSSEDMNGLSSQLVFSDEAMPFNCANTPQRRVGDPGPKTRELCGFMDDKLFPVDRDRFFA 60
           MSSEDMNGLSSQLVFSDEAMPFNCANTPQRRVGDPGPKTRELCGFMDDKLFPVDRDRFFA
Sbjct: 1   MSSEDMNGLSSQLVFSDEAMPFNCANTPQRRVGDPGPKTRELCGFMDDKLFPVDRDRFFA 60

Query: 61  HQGADFRRSVFSDSRNWNGNGSARSGSGKGSEEE-DDDDDDDEENEVDDGDDDVEGLVSM 120
           HQGADFRRSVFSDSRNWNGNGSARSGSGKGSEEE DDDDDDDEENEVDDGDDDVEGLVSM
Sbjct: 61  HQGADFRRSVFSDSRNWNGNGSARSGSGKGSEEEDDDDDDDDEENEVDDGDDDVEGLVSM 120

Query: 121 DDVNKCNKMANPNSSNSDRNDQSSGGLKNGTNKLGNGKPKHHSSFGGTLLDKDGMVGPSA 180
           DDVNKCNKMANPNSSNSDRNDQSSGGLKNGTNKLGNGKPKHHSSFGGTLL KDGMVGPSA
Sbjct: 121 DDVNKCNKMANPNSSNSDRNDQSSGGLKNGTNKLGNGKPKHHSSFGGTLLVKDGMVGPSA 180

Query: 181 SNAGATQSENHPQARLGNYPNAVTIAEPDGDAYYSQYLQGTEGSASGQKDLGMENGCGFN 240
           SNAGATQSENHPQARLGNYPNAVTIAEPDGDAYYSQYLQGTEGSASGQKDLGMENGCGFN
Sbjct: 181 SNAGATQSENHPQARLGNYPNAVTIAEPDGDAYYSQYLQGTEGSASGQKDLGMENGCGFN 240

Query: 241 GRRDVSISSEPGESLRTILADPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCSQS 300
           GRRD SISSEPGESLRTILADPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCSQS
Sbjct: 241 GRRDASISSEPGESLRTILADPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCSQS 300

Query: 301 VSEDSIAQNLSLQAAVQAFRREEEILFYRSPKRRRERFEQDKGGYGDLTLMDTPRGRGVQ 360
           VSEDSIAQNLSLQAAVQAFRREEEILFYRSPKRRRERFEQDKGGYGDLTLMDTPRGRGVQ
Sbjct: 301 VSEDSIAQNLSLQAAVQAFRREEEILFYRSPKRRRERFEQDKGGYGDLTLMDTPRGRGVQ 360

Query: 361 FPFVVTDRVIIKVSRKLYSHLVLYYQILKLISTKMDQGNKRTPQRFVGREAVVTTQCLNG 420
           FPFVVTDRVIIK                         GNKRTPQRFVGREAVVTTQCLNG
Sbjct: 361 FPFVVTDRVIIK-------------------------GNKRTPQRFVGREAVVTTQCLNG 420

Query: 421 WYVVKTLDNAESVKLQYRSLAKVPNDAASKPIPRKIAPNWL 461
           WYVVKTLDNAESVKLQYRSLAKVPNDAASKPIPRKIAPNWL
Sbjct: 421 WYVVKTLDNAESVKLQYRSLAKVPNDAASKPIPRKIAPNWL 436

BLAST of MS020766 vs. ExPASy TrEMBL
Match: A0A6J1CYI7 (U-box domain-containing protein 62 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111015962 PE=4 SV=1)

HSP 1 Score: 823.5 bits (2126), Expect = 4.2e-235
Identity = 431/461 (93.49%), Postives = 431/461 (93.49%), Query Frame = 0

Query: 1   MSSEDMNGLSSQLVFSDEAMPFNCANTPQRRVGDPGPKTRELCGFMDDKLFPVDRDRFFA 60
           MSSEDMNGLSSQLVFSDEAMPFNCANTPQRRVGDPGPKTRELCGFMDDKLFPVDRDRFFA
Sbjct: 1   MSSEDMNGLSSQLVFSDEAMPFNCANTPQRRVGDPGPKTRELCGFMDDKLFPVDRDRFFA 60

Query: 61  HQGADFRRSVFSDSRNWNGNGSARSGSGKGSEEE-DDDDDDDEENEVDDGDDDVEGLVSM 120
           HQGADFRRSVFSDSRNW  NGSARSGSGKGSEEE DDDDDDDEENEVDDGDDDVEGLVSM
Sbjct: 61  HQGADFRRSVFSDSRNW--NGSARSGSGKGSEEEDDDDDDDDEENEVDDGDDDVEGLVSM 120

Query: 121 DDVNKCNKMANPNSSNSDRNDQSSGGLKNGTNKLGNGKPKHHSSFGGTLLDKDGMVGPSA 180
           DDVNKCNKMANPNSSNSDRNDQSSGGLKNGTNKLGNGKPKHHSSFGGTLL KDGMVGPSA
Sbjct: 121 DDVNKCNKMANPNSSNSDRNDQSSGGLKNGTNKLGNGKPKHHSSFGGTLLVKDGMVGPSA 180

Query: 181 SNAGATQSENHPQARLGNYPNAVTIAEPDGDAYYSQYLQGTEGSASGQKDLGMENGCGFN 240
           SNAGATQSENHPQARLGNYPNAVTIAEPDGDAYYSQYLQGTEGSASGQKDLGMENGCGFN
Sbjct: 181 SNAGATQSENHPQARLGNYPNAVTIAEPDGDAYYSQYLQGTEGSASGQKDLGMENGCGFN 240

Query: 241 GRRDVSISSEPGESLRTILADPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCSQS 300
           GRRD SISSEPGESLRTILADPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCSQS
Sbjct: 241 GRRDASISSEPGESLRTILADPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCSQS 300

Query: 301 VSEDSIAQNLSLQAAVQAFRREEEILFYRSPKRRRERFEQDKGGYGDLTLMDTPRGRGVQ 360
           VSEDSIAQNLSLQAAVQAFRREEEILFYRSPKRRRERFEQDKGGYGDLTLMDTPRGRGVQ
Sbjct: 301 VSEDSIAQNLSLQAAVQAFRREEEILFYRSPKRRRERFEQDKGGYGDLTLMDTPRGRGVQ 360

Query: 361 FPFVVTDRVIIKVSRKLYSHLVLYYQILKLISTKMDQGNKRTPQRFVGREAVVTTQCLNG 420
           FPFVVTDRVIIK                         GNKRTPQRFVGREAVVTTQCLNG
Sbjct: 361 FPFVVTDRVIIK-------------------------GNKRTPQRFVGREAVVTTQCLNG 420

Query: 421 WYVVKTLDNAESVKLQYRSLAKVPNDAASKPIPRKIAPNWL 461
           WYVVKTLDNAESVKLQYRSLAKVPNDAASKPIPRKIAPNWL
Sbjct: 421 WYVVKTLDNAESVKLQYRSLAKVPNDAASKPIPRKIAPNWL 434

BLAST of MS020766 vs. ExPASy TrEMBL
Match: A0A6J1FV24 (U-box domain-containing protein 62 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447538 PE=4 SV=1)

HSP 1 Score: 680.2 bits (1754), Expect = 5.8e-192
Identity = 367/463 (79.27%), Postives = 386/463 (83.37%), Query Frame = 0

Query: 1   MSSEDMNGLSSQLVFSDEAMPFNCANTPQRRVGDPGPKTRELCGFMDDKLFPVDRDRFFA 60
           MSSEDMNGL+S LVF ++AMPFNCA T QRRV DP PKTRELCGFMDDKL PVDRDRFF 
Sbjct: 1   MSSEDMNGLNSHLVFQEDAMPFNCATTSQRRVADPAPKTRELCGFMDDKLLPVDRDRFFV 60

Query: 61  HQGADFRRSVFSDSRNWNGNGSARSGSGKGSEEEDDDD-DDDEENEVDDGDDD--VEGLV 120
           HQG DFRRSVF  SRNWNGN SAR+GSGKGS+EEDDDD DDD+E+EVDDGDDD  VEGL 
Sbjct: 61  HQGEDFRRSVFGHSRNWNGNESARTGSGKGSDEEDDDDEDDDDEDEVDDGDDDVVVEGLA 120

Query: 121 SMDDVNKCNKMANPNSSNSDRNDQSSGGLKNGTNKLGNGKPKHHSSFGGTLLDKDGMVGP 180
           S+DDVNKC K ANPN +N D +DQSS GLKNGTNKLGNGK KHHSSFGGTLL KDG+VG 
Sbjct: 121 SLDDVNKCTKTANPNITNKDISDQSSDGLKNGTNKLGNGKLKHHSSFGGTLLVKDGLVGS 180

Query: 181 SASNAGATQSENHPQARLGNYPNAVTIAEPDGDAYYSQYLQGTEGSASGQKDLGMENGCG 240
           S  N GAT+          N PNAVTIAEP+ +AYYSQYLQGTEGS SGQKDL +ENGCG
Sbjct: 181 SGCNTGATR----------NDPNAVTIAEPE-NAYYSQYLQGTEGSTSGQKDLAIENGCG 240

Query: 241 FNGRRDVSISSEPGESLRTILADPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCS 300
           F GRRDVS SSEP ESLR IL DPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCS
Sbjct: 241 F-GRRDVSFSSEPEESLRAILTDPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCS 300

Query: 301 QSVSEDSIAQNLSLQAAVQAFRREEEILFYRSPKRRRERFEQDKGGYGDLTLMDTPRGRG 360
           QSVSEDSIAQNLSLQAAVQAFRREEE+LFYRSPKRRRERFEQDKGGYGD TLMDTPRGRG
Sbjct: 301 QSVSEDSIAQNLSLQAAVQAFRREEELLFYRSPKRRRERFEQDKGGYGDSTLMDTPRGRG 360

Query: 361 VQFPFVVTDRVIIKVSRKLYSHLVLYYQILKLISTKMDQGNKRTPQRFVGREAVVTTQCL 420
           VQFPF VTDRVIIK                         GNKRTPQRFVGREAVVT QCL
Sbjct: 361 VQFPFAVTDRVIIK-------------------------GNKRTPQRFVGREAVVTAQCL 420

Query: 421 NGWYVVKTLDNAESVKLQYRSLAKVPNDAASKPIPRKIAPNWL 461
           NGWYVVKTLDNAESVKLQYRSLA+VPNDAASK I +K+APNWL
Sbjct: 421 NGWYVVKTLDNAESVKLQYRSLARVPNDAASKTITKKVAPNWL 426

BLAST of MS020766 vs. ExPASy TrEMBL
Match: A0A0A0LIQ7 (U-box domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G852550 PE=4 SV=1)

HSP 1 Score: 676.0 bits (1743), Expect = 1.1e-190
Identity = 360/461 (78.09%), Postives = 390/461 (84.60%), Query Frame = 0

Query: 1   MSSEDMNGLSSQLVFSDEAMPFNCANTPQRRVGDPGPKTRELCGFMDDKLFP-VDRDRFF 60
           MSSED+NGL S LVF +E++PFNC++TP RR+ DP PKTRELCGFMDDKLFP VDRDRFF
Sbjct: 1   MSSEDINGLDSHLVFQNESLPFNCSSTPHRRLPDPPPKTRELCGFMDDKLFPIVDRDRFF 60

Query: 61  AHQGADFRRSVFSDSRNWNGNGSARSGSGKGSEEEDDDDDDDEENEVDDGDDDVEGLVSM 120
           + QG DFRR+VF  +RNW  NGSAR+GSGKGS+EEDDDDDDD+E+EVDDGDD V+GLVS+
Sbjct: 61  SQQGEDFRRNVFGHNRNW--NGSARTGSGKGSDEEDDDDDDDDEDEVDDGDDGVKGLVSL 120

Query: 121 DDVNKCNKMANPNSSNSDRNDQSSGGLKNGTNKLGNGKPKHHSSFGGTLLDKDGMVGPSA 180
           DDVNKC+K ANP  SN+DR+DQSS GLKNG NKLGNGK K HSSF GTLL KD  VG S 
Sbjct: 121 DDVNKCSKTANPIDSNNDRSDQSSEGLKNGINKLGNGKLKQHSSFCGTLLVKDAFVGSS- 180

Query: 181 SNAGATQSENHPQARLGNYPNAVTIAEPDGDAYYSQYLQGTEGSASGQKDLGMENGCGFN 240
              GATQSEN+PQ RLGNYPNAVT+AEPD +AYYSQYLQ  EGSASGQK+L MENGCGF 
Sbjct: 181 ---GATQSENNPQGRLGNYPNAVTVAEPDSEAYYSQYLQSNEGSASGQKNLSMENGCGF- 240

Query: 241 GRRDVSISSEPGESLRTILADPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCSQS 300
           GRRDVS S EPGESLR+IL DPVTGALM+DAMILPCGHSFGSGGIQHVL+V+SCYSCSQS
Sbjct: 241 GRRDVSFSGEPGESLRSILTDPVTGALMNDAMILPCGHSFGSGGIQHVLKVRSCYSCSQS 300

Query: 301 VSEDSIAQNLSLQAAVQAFRREEEILFYRSPKRRRERFEQDKGGYGDLTLMDTPRGRGVQ 360
           VSEDSIAQNLSLQAAVQAF REEE+LF+RSPKRRRERFEQDKGGYGDLTLMDT RGRGVQ
Sbjct: 301 VSEDSIAQNLSLQAAVQAFCREEELLFHRSPKRRRERFEQDKGGYGDLTLMDTQRGRGVQ 360

Query: 361 FPFVVTDRVIIKVSRKLYSHLVLYYQILKLISTKMDQGNKRTPQRFVGREAVVTTQCLNG 420
           FPF VTDRVIIK                         GNKRTPQRFVGREAVVTTQCLNG
Sbjct: 361 FPFAVTDRVIIK-------------------------GNKRTPQRFVGREAVVTTQCLNG 420

Query: 421 WYVVKTLDNAESVKLQYRSLAKVPNDAASKPIPRKIAPNWL 461
           WYVVKTLDNAESVKLQYRSLAKVPNDA +K I RKI PNWL
Sbjct: 421 WYVVKTLDNAESVKLQYRSLAKVPNDATTKTITRKITPNWL 429

BLAST of MS020766 vs. ExPASy TrEMBL
Match: A0A5D3BAD9 (U-box domain-containing protein 62-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold233G00200 PE=4 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 4.2e-190
Identity = 357/461 (77.44%), Postives = 388/461 (84.16%), Query Frame = 0

Query: 1   MSSEDMNGLSSQLVFSDEAMPFNCANTPQRRVGDPGPKTRELCGFMDDKLFP-VDRDRFF 60
           MSS+ +NGL+S L+F DE++PFNC++TP RR+ DP PKTRELCGFMDDKLFP VDRDRFF
Sbjct: 1   MSSQHINGLNSHLLFQDESLPFNCSSTPHRRLPDPPPKTRELCGFMDDKLFPVVDRDRFF 60

Query: 61  AHQGADFRRSVFSDSRNWNGNGSARSGSGKGSEEEDDDDDDDEENEVDDGDDDVEGLVSM 120
           + QG DFRRSVF  +RNW  NG+AR+GSGKGS+EEDDDDDDD+E+EVDDGD  ++GLVS+
Sbjct: 61  SQQGEDFRRSVFGPNRNW--NGTARTGSGKGSDEEDDDDDDDDEDEVDDGDAGLKGLVSL 120

Query: 121 DDVNKCNKMANPNSSNSDRNDQSSGGLKNGTNKLGNGKPKHHSSFGGTLLDKDGMVGPSA 180
           +DVNKC+K  NP  SN+DR+DQSSGGLKNGTNKLGNGK K HSSFGGTLL KD +VG S 
Sbjct: 121 NDVNKCSKTGNPTDSNNDRSDQSSGGLKNGTNKLGNGKLKQHSSFGGTLLVKDALVGSS- 180

Query: 181 SNAGATQSENHPQARLGNYPNAVTIAEPDGDAYYSQYLQGTEGSASGQKDLGMENGCGFN 240
              GA+QSEN+PQ RLGNYPNAVT+AEPD +AYYSQYLQ  EGSAS QKDL MENGCGF 
Sbjct: 181 ---GASQSENNPQGRLGNYPNAVTVAEPDSEAYYSQYLQSNEGSASEQKDLSMENGCGF- 240

Query: 241 GRRDVSISSEPGESLRTILADPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCSQS 300
           GRRDV  S EPGESLR IL DPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCSQS
Sbjct: 241 GRRDVLFSGEPGESLRAILTDPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCSQS 300

Query: 301 VSEDSIAQNLSLQAAVQAFRREEEILFYRSPKRRRERFEQDKGGYGDLTLMDTPRGRGVQ 360
           VSEDSIAQNLSLQAAV+AFRREEE+LFY SPKRRRERFEQDKGGYGDLTLM T RGRGVQ
Sbjct: 301 VSEDSIAQNLSLQAAVRAFRREEELLFYHSPKRRRERFEQDKGGYGDLTLMATQRGRGVQ 360

Query: 361 FPFVVTDRVIIKVSRKLYSHLVLYYQILKLISTKMDQGNKRTPQRFVGREAVVTTQCLNG 420
           FPF VTDRVIIK                         GNKRTPQRFVGREAVVTTQCLNG
Sbjct: 361 FPFAVTDRVIIK-------------------------GNKRTPQRFVGREAVVTTQCLNG 420

Query: 421 WYVVKTLDNAESVKLQYRSLAKVPNDAASKPIPRKIAPNWL 461
           WYVVKTLDNAESVKLQYRSLAKVPNDA SK I RK+ PNWL
Sbjct: 421 WYVVKTLDNAESVKLQYRSLAKVPNDARSKTITRKVTPNWL 429

BLAST of MS020766 vs. TAIR 10
Match: AT5G05230.1 (RING/U-box superfamily protein )

HSP 1 Score: 257.7 bits (657), Expect = 1.8e-68
Identity = 141/264 (53.41%), Postives = 170/264 (64.39%), Query Frame = 0

Query: 201 AVTIAEPDGDAYYSQYLQGTEG---SASGQKDLGMENGCGFNGRRDVSISSEPG-ESLRT 260
           AV +   D  AYYSQ+ +  E    S + +  +  +NGC F+GRRD+S SS    ESLRT
Sbjct: 125 AVIVVGQDNAAYYSQHFKTVEASFVSRNEESSIAADNGCDFSGRRDLSSSSSNSIESLRT 184

Query: 261 ILADPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCSQSVSEDSIAQNLSLQAAVQ 320
           IL+DP TG+LM DAMILPCGH+FG+GGI+ V ++K+C +CSQ VSEDSI  NL+L+ AVQ
Sbjct: 185 ILSDPTTGSLMADAMILPCGHTFGAGGIEQVKQMKACCTCSQPVSEDSITPNLTLRVAVQ 244

Query: 321 AFRREEEILFYRSPKRRRERFEQDKGGYGDLTLMDTPRGRGVQFPFVVTDRVIIKVSRKL 380
           AF REE        KR+RE F+Q++  +G        R +   FPF V DRVIIK     
Sbjct: 245 AFCREENSQSNHPSKRKREGFDQERRAFGVTNHSGRSRNKSNHFPFAVADRVIIK----- 304

Query: 381 YSHLVLYYQILKLISTKMDQGNKRTPQRFVGREAVVTTQCLNGWYVVKTLDNAESVKLQY 440
                               GNKRTP RFVGREAVVTTQCLNGWYVVKTLDNAESVKLQY
Sbjct: 305 --------------------GNKRTPPRFVGREAVVTTQCLNGWYVVKTLDNAESVKLQY 363

Query: 441 RSLAKVPNDAASKPIPRKIAPNWL 461
           RSLAK P D ++K  P K+  NWL
Sbjct: 365 RSLAKAPEDPSAKATPNKMVSNWL 363

BLAST of MS020766 vs. TAIR 10
Match: AT2G40640.1 (RING/U-box superfamily protein )

HSP 1 Score: 194.1 bits (492), Expect = 2.4e-49
Identity = 114/261 (43.68%), Postives = 159/261 (60.92%), Query Frame = 0

Query: 202 VTIAEPDGDAYYS-QYLQGTEGSASGQKDLGMENGCGFNGRRDVSISSEPGESLRTILAD 261
           V I +PDG+   S +  Q     AS +K+            R  + S    ESL+ IL+D
Sbjct: 161 VRITKPDGNVSNSHRNTQQKRDFASVEKE------------RVTTTSVSSWESLKAILSD 220

Query: 262 PVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCSQSVSEDSIAQNLSLQAAVQAFRR 321
           PVTGALM+DA ILPCGHSFG+GG++ V ++K+C++CSQ   E S   NLSL+  V AFR+
Sbjct: 221 PVTGALMNDATILPCGHSFGAGGLKEVKKMKACFTCSQPTLEGSEKPNLSLRIVVHAFRQ 280

Query: 322 EEEILFYRSPKRRRERFEQDKGGYGDLTLMDTPR-GRGVQFPFVVTDRVIIKVSRKLYSH 381
           EE+     + KRR+ER +Q K  +    + +TP+  RG+QFPF + D +II         
Sbjct: 281 EEDSDHIHTLKRRKERSDQ-KRSFCIPNITETPKSSRGIQFPFSIGDHIII--------- 340

Query: 382 LVLYYQILKLISTKMDQGNKRTPQRFVGREAVVTTQCLNGWYVVKTLDNAESVKLQYRSL 441
                           +GNKRTP RFVGR AV+ TQCLNGWYVVKT+DN+ES+KLQ+ SL
Sbjct: 341 ----------------EGNKRTPPRFVGRIAVIMTQCLNGWYVVKTVDNSESIKLQHCSL 383

Query: 442 AKVPNDAASKPIPRKIAPNWL 461
           AK+ +++++K    ++ P+WL
Sbjct: 401 AKISDNSSTKVTVAEMPPSWL 383

BLAST of MS020766 vs. TAIR 10
Match: AT2G40640.2 (RING/U-box superfamily protein )

HSP 1 Score: 151.4 bits (381), Expect = 1.8e-36
Identity = 94/221 (42.53%), Postives = 126/221 (57.01%), Query Frame = 0

Query: 202 VTIAEPDGDAYYS-QYLQGTEGSASGQKDLGMENGCGFNGRRDVSISSEPGESLRTILAD 261
           V I +PDG+   S +  Q     AS +K+            R  + S    ESL+ IL+D
Sbjct: 161 VRITKPDGNVSNSHRNTQQKRDFASVEKE------------RVTTTSVSSWESLKAILSD 220

Query: 262 PVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCSQSVSEDSIAQNLSLQAAVQAFRR 321
           PVTGALM+DA ILPCGHSFG+GG++ V ++K+C++CSQ   E S   NLSL+  V AFR+
Sbjct: 221 PVTGALMNDATILPCGHSFGAGGLKEVKKMKACFTCSQPTLEGSEKPNLSLRIVVHAFRQ 280

Query: 322 EEEILFYRSPKRRRERFEQDKGGYGDLTLMDTPR-GRGVQFPFVVTDRVIIKVSRKLYSH 381
           EE+     + KRR+ER +Q K  +    + +TP+  RG+QFPF + D +II         
Sbjct: 281 EEDSDHIHTLKRRKERSDQ-KRSFCIPNITETPKSSRGIQFPFSIGDHIII--------- 340

Query: 382 LVLYYQILKLISTKMDQGNKRTPQRFVGREAVVTTQCLNGW 421
                           +GNKRTP RFVGR AV+ TQCLNGW
Sbjct: 341 ----------------EGNKRTPPRFVGRIAVIMTQCLNGW 343

BLAST of MS020766 vs. TAIR 10
Match: AT3G19895.1 (RING/U-box superfamily protein )

HSP 1 Score: 140.2 bits (352), Expect = 4.2e-33
Identity = 76/203 (37.44%), Postives = 122/203 (60.10%), Query Frame = 0

Query: 246 ISSEPGESLRTILADPVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCSQSVSEDSI 305
           +  EP  SLR++L+DP++GA ++DAM++ CGHSFG   ++ VL +  C  C+  +   S+
Sbjct: 364 VCKEP--SLRSVLSDPISGAFLEDAMVVSCGHSFGGLMLRRVLEMSRCTLCNTEIESGSL 423

Query: 306 AQNLSLQAAVQAFRREEE-ILFYRSPKRRRERFEQDKGGY--GDLTLMDTPRGRGVQFPF 365
             N +L+AA  A +++++  LF+ +  RRR +   D+     GD    D    R V +PF
Sbjct: 424 VPNHALRAAASAIKQQDDKRLFHNAAMRRRRKEMSDQMDVENGD-PATDDGMHRVVHYPF 483

Query: 366 VVTDRVIIKVSRKLYSHLVLYYQILKLISTKMDQGNKRTPQRFVGREAVVTTQCLNGWYV 425
            V ++V+IK                         GN+RTP++FVG+EA+VT+QCLNGWY+
Sbjct: 484 AVNEKVLIK-------------------------GNRRTPEKFVGKEAIVTSQCLNGWYL 538

Query: 426 VKTLDNAESVKLQYRSLAKVPND 446
           +K +++ ++V+LQYRSL K+ ND
Sbjct: 544 LKIVESGDNVRLQYRSLKKMVND 538

BLAST of MS020766 vs. TAIR 10
Match: AT2G40640.3 (RING/U-box superfamily protein )

HSP 1 Score: 101.7 bits (252), Expect = 1.6e-21
Identity = 62/138 (44.93%), Postives = 84/138 (60.87%), Query Frame = 0

Query: 202 VTIAEPDGDAYYS-QYLQGTEGSASGQKDLGMENGCGFNGRRDVSISSEPGESLRTILAD 261
           V I +PDG+   S +  Q     AS +K+            R  + S    ESL+ IL+D
Sbjct: 161 VRITKPDGNVSNSHRNTQQKRDFASVEKE------------RVTTTSVSSWESLKAILSD 220

Query: 262 PVTGALMDDAMILPCGHSFGSGGIQHVLRVKSCYSCSQSVSEDSIAQNLSLQAAVQAFRR 321
           PVTGALM+DA ILPCGHSFG+GG++ V ++K+C++CSQ   E S   NLSL+  V AFR+
Sbjct: 221 PVTGALMNDATILPCGHSFGAGGLKEVKKMKACFTCSQPTLEGSEKPNLSLRIVVHAFRQ 280

Query: 322 EEEILFYRSPKRRRERFE 339
           EE+     + KRR+ER E
Sbjct: 281 EEDSDHIHTLKRRKERRE 286

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022146864.11.9e-23793.93U-box domain-containing protein 62 isoform X1 [Momordica charantia][more]
XP_022146865.18.8e-23593.49U-box domain-containing protein 62 isoform X2 [Momordica charantia][more]
XP_038892965.13.6e-19680.69U-box domain-containing protein 62-like [Benincasa hispida][more]
XP_022942528.11.2e-19179.27U-box domain-containing protein 62 isoform X1 [Cucurbita moschata] >KAG6600875.1... [more]
XP_004147826.31.2e-19178.52U-box domain-containing protein 62 isoform X1 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
Q6DBN52.5e-6753.41U-box domain-containing protein 62 OS=Arabidopsis thaliana OX=3702 GN=PUB62 PE=2... [more]
Q58FY43.4e-4843.68U-box domain-containing protein 63 OS=Arabidopsis thaliana OX=3702 GN=PUB63 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1CZA99.1e-23893.93U-box domain-containing protein 62 isoform X1 OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1CYI74.2e-23593.49U-box domain-containing protein 62 isoform X2 OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1FV245.8e-19279.27U-box domain-containing protein 62 isoform X1 OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A0A0LIQ71.1e-19078.09U-box domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G852550 PE=4 ... [more]
A0A5D3BAD94.2e-19077.44U-box domain-containing protein 62-like isoform X1 OS=Cucumis melo var. makuwa O... [more]
Match NameE-valueIdentityDescription
AT5G05230.11.8e-6853.41RING/U-box superfamily protein [more]
AT2G40640.12.4e-4943.68RING/U-box superfamily protein [more]
AT2G40640.21.8e-3642.53RING/U-box superfamily protein [more]
AT3G19895.14.2e-3337.44RING/U-box superfamily protein [more]
AT2G40640.31.6e-2144.93RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 253..324
e-value: 2.5E-9
score: 38.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 69..87
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 179..195
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 125..150
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 92..116
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 69..195
NoneNo IPR availablePANTHERPTHR33644:SF5U-BOX DOMAIN-CONTAINING PROTEIN 62coord: 43..460
NoneNo IPR availablePANTHERPTHR33644U-BOX DOMAIN-CONTAINING PROTEIN 62-RELATEDcoord: 43..460
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 253..326
IPR003613U box domainPROSITEPS51698U_BOXcoord: 253..325
score: 8.750163

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS020766.1MS020766.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity