Homology
BLAST of MS020748 vs. NCBI nr
Match:
XP_022146805.1 (probable RNA-dependent RNA polymerase 1 isoform X1 [Momordica charantia])
HSP 1 Score: 2278.4 bits (5903), Expect = 0.0e+00
Identity = 1124/1172 (95.90%), Postives = 1134/1172 (96.76%), Query Frame = 0
Query: 1 MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60
MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE
Sbjct: 1 MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60
Query: 61 LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120
LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW
Sbjct: 61 LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120
Query: 121 EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLGSN 180
EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVI
Sbjct: 121 EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVI------ 180
Query: 181 SFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVPDD 240
QLYGAP IYKKVESSSGSR+VFDDPLLNYFKEVPDD
Sbjct: 181 ------------------------QLYGAPWIYKKVESSSGSRQVFDDPLLNYFKEVPDD 240
Query: 241 QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSCSA 300
QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFR VDGGNGFSCSA
Sbjct: 241 QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRLVDGGNGFSCSA 300
Query: 301 DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSRIKIEFVEHALDR 360
DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDP RIKIEFVEHALDR
Sbjct: 301 DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPRRIKIEFVEHALDR 360
Query: 361 LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420
LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV
Sbjct: 361 LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420
Query: 421 SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI 480
SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI
Sbjct: 421 SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI 480
Query: 481 VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPAKYAARLGQSF 540
VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDA DIRAWMGDFRQIKNPAKYAARLGQSF
Sbjct: 481 VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAEDIRAWMGDFRQIKNPAKYAARLGQSF 540
Query: 541 GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF 600
GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF
Sbjct: 541 GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF 600
Query: 601 QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660
QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST
Sbjct: 601 QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660
Query: 661 LGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGYKPNVEPY 720
LGVRDEVFEKKQ EAV+QLDAILTDPLKAQEAL+LMAPGENTNVLKEMLKCGYKPNVEPY
Sbjct: 661 LGVRDEVFEKKQREAVEQLDAILTDPLKAQEALELMAPGENTNVLKEMLKCGYKPNVEPY 720
Query: 721 LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTKQSILSVT 780
LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSG KQSILSVT
Sbjct: 721 LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGAKQSILSVT 780
Query: 781 TSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDCVVFPQKGSRPH 840
TSAFN++SSEQRVAI+GNVVVAKNPCLHPGD+RVLKAVNVP+LYHMVDCVVFPQKGSRPH
Sbjct: 781 TSAFNISSSEQRVAIEGNVVVAKNPCLHPGDVRVLKAVNVPRLYHMVDCVVFPQKGSRPH 840
Query: 841 PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN 900
PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN
Sbjct: 841 PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN 900
Query: 901 DSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF 960
DSLGIIANAHTAFADKELFKARSSPCI LAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF
Sbjct: 901 DSLGIIANAHTAFADKELFKARSSPCINLAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF 960
Query: 961 MDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA 1020
M+KPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA
Sbjct: 961 MEKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA 1020
Query: 1021 IYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART 1080
IY+KNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART
Sbjct: 1021 IYHKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART 1080
Query: 1081 WFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL 1140
WFNERESGPDSDSV GLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL
Sbjct: 1081 WFNERESGPDSDSVSGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL 1140
Query: 1141 MQIKENNVRRKQRASRLAALDSRFRNALNLGH 1173
MQIKENNVRRKQRASRLAALDSRFRNALNLGH
Sbjct: 1141 MQIKENNVRRKQRASRLAALDSRFRNALNLGH 1142
BLAST of MS020748 vs. NCBI nr
Match:
XP_022146806.1 (probable RNA-dependent RNA polymerase 1 isoform X2 [Momordica charantia])
HSP 1 Score: 2191.8 bits (5678), Expect = 0.0e+00
Identity = 1091/1172 (93.09%), Postives = 1101/1172 (93.94%), Query Frame = 0
Query: 1 MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60
MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE
Sbjct: 1 MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60
Query: 61 LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120
LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW
Sbjct: 61 LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120
Query: 121 EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLGSN 180
EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVI
Sbjct: 121 EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVI------ 180
Query: 181 SFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVPDD 240
QLYGAP IYKKVESSSGSR+VFDDPLLNYFKEVPDD
Sbjct: 181 ------------------------QLYGAPWIYKKVESSSGSRQVFDDPLLNYFKEVPDD 240
Query: 241 QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSCSA 300
QW NFAYYKETEDEFR VDGGNGFSCSA
Sbjct: 241 QW---------------------------------NFAYYKETEDEFRLVDGGNGFSCSA 300
Query: 301 DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSRIKIEFVEHALDR 360
DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDP RIKIEFVEHALDR
Sbjct: 301 DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPRRIKIEFVEHALDR 360
Query: 361 LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420
LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV
Sbjct: 361 LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420
Query: 421 SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI 480
SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI
Sbjct: 421 SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI 480
Query: 481 VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPAKYAARLGQSF 540
VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDA DIRAWMGDFRQIKNPAKYAARLGQSF
Sbjct: 481 VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAEDIRAWMGDFRQIKNPAKYAARLGQSF 540
Query: 541 GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF 600
GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF
Sbjct: 541 GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF 600
Query: 601 QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660
QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST
Sbjct: 601 QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660
Query: 661 LGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGYKPNVEPY 720
LGVRDEVFEKKQ EAV+QLDAILTDPLKAQEAL+LMAPGENTNVLKEMLKCGYKPNVEPY
Sbjct: 661 LGVRDEVFEKKQREAVEQLDAILTDPLKAQEALELMAPGENTNVLKEMLKCGYKPNVEPY 720
Query: 721 LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTKQSILSVT 780
LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSG KQSILSVT
Sbjct: 721 LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGAKQSILSVT 780
Query: 781 TSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDCVVFPQKGSRPH 840
TSAFN++SSEQRVAI+GNVVVAKNPCLHPGD+RVLKAVNVP+LYHMVDCVVFPQKGSRPH
Sbjct: 781 TSAFNISSSEQRVAIEGNVVVAKNPCLHPGDVRVLKAVNVPRLYHMVDCVVFPQKGSRPH 840
Query: 841 PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN 900
PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN
Sbjct: 841 PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN 900
Query: 901 DSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF 960
DSLGIIANAHTAFADKELFKARSSPCI LAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF
Sbjct: 901 DSLGIIANAHTAFADKELFKARSSPCINLAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF 960
Query: 961 MDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA 1020
M+KPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA
Sbjct: 961 MEKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA 1020
Query: 1021 IYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART 1080
IY+KNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART
Sbjct: 1021 IYHKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART 1080
Query: 1081 WFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL 1140
WFNERESGPDSDSV GLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL
Sbjct: 1081 WFNERESGPDSDSVSGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL 1109
Query: 1141 MQIKENNVRRKQRASRLAALDSRFRNALNLGH 1173
MQIKENNVRRKQRASRLAALDSRFRNALNLGH
Sbjct: 1141 MQIKENNVRRKQRASRLAALDSRFRNALNLGH 1109
BLAST of MS020748 vs. NCBI nr
Match:
XP_038891504.1 (RNA-dependent RNA polymerase 1 isoform X1 [Benincasa hispida] >XP_038891505.1 RNA-dependent RNA polymerase 1 isoform X2 [Benincasa hispida])
HSP 1 Score: 1892.9 bits (4902), Expect = 0.0e+00
Identity = 934/1171 (79.76%), Postives = 1026/1171 (87.62%), Query Frame = 0
Query: 1 MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60
MGKTVHISGFP+ VTADAV+S+LEGRTGVGTVYA+K+R PK+GG+R+YA VQFT + +AE
Sbjct: 1 MGKTVHISGFPSNVTADAVKSFLEGRTGVGTVYAIKVRAPKRGGARVYAIVQFTSATQAE 60
Query: 61 LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120
LIISLAN Q LWYG+SYLKAR A+VDI+PKPRTYM+TLEDL LCFGCQVSSEKF VLW
Sbjct: 61 LIISLAN---QSLWYGSSYLKARAAEVDIVPKPRTYMYTLEDLPLCFGCQVSSEKFRVLW 120
Query: 121 EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLGSN 180
EG LVTFG GMRKMNF L HN V+YR ELSYENIW+I+LHH +H+SMKYL+I
Sbjct: 121 EGDIDLVTFGIGMRKMNFHLKHNSVKYRLELSYENIWQIQLHHPQHRSMKYLII------ 180
Query: 181 SFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVPDD 240
QLYGAPRIYK V + S ++FDDPL N+F EVPDD
Sbjct: 181 ------------------------QLYGAPRIYKNV--APCSVQIFDDPLFNFFMEVPDD 240
Query: 241 QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSCSA 300
QW+R+ DFTPS IGQSSSLCLKL N +LPNF QNFAYY+E E+EF+ VDG FS
Sbjct: 241 QWIRTADFTPSCSIGQSSSLCLKLPNRCQLPNFRQNFAYYEEFENEFQLVDGDASFSFCR 300
Query: 301 DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSRIKIEFVEHALDR 360
DL P+VDSRP + LPY+I+FKINALVQHGCI P +DTSFY LVDP RI+IEFVEHAL++
Sbjct: 301 DLAPIVDSRPHV-LPYEIMFKINALVQHGCIPWPLIDTSFYRLVDPRRIRIEFVEHALEK 360
Query: 361 LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420
LFHLKEC Y+P S++TEQY+KY R PPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV
Sbjct: 361 LFHLKECSYEPSSFITEQYRKYSRHPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420
Query: 421 SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI 480
SNRVLRHF +D DNFLRVSFVDEEWDKMRS DLLPR S T +D KT+IYKRILSVL+NGI
Sbjct: 421 SNRVLRHFPQDTDNFLRVSFVDEEWDKMRSTDLLPRMSPTSEDSKTDIYKRILSVLRNGI 480
Query: 481 VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPAKYAARLGQSF 540
VIG K F+FLAFSSSQLRDNSLWMFA+RPGLDAADIRAWMGDFR IKNPAKYAARLGQSF
Sbjct: 481 VIGDKTFQFLAFSSSQLRDNSLWMFASRPGLDAADIRAWMGDFRHIKNPAKYAARLGQSF 540
Query: 541 GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF 600
GSSTETLSVAR EREIIPDIE+QHGEVKYVFSDGIGKIS+DFAKKV+AKCG + TPSAF
Sbjct: 541 GSSTETLSVARDEREIIPDIELQHGEVKYVFSDGIGKISNDFAKKVAAKCGFQGSTPSAF 600
Query: 601 QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660
QIRYGGYKGVVAVDP+SS KLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST
Sbjct: 601 QIRYGGYKGVVAVDPHSSIKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660
Query: 661 LGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGYKPNVEPY 720
LGVRDE+FE KQ EAV+QLDAILTDPLKA+EAL+LMAPGENTN+LKEMLKCGYKP+VEPY
Sbjct: 661 LGVRDEIFENKQREAVEQLDAILTDPLKAREALELMAPGENTNILKEMLKCGYKPDVEPY 720
Query: 721 LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTKQSILSVT 780
LSMMLQ FRASKLL+LRTKSR+FIPNGRAMMGCLDET +LEYGQVFVQ+S ++ LS
Sbjct: 721 LSMMLQTFRASKLLELRTKSRIFIPNGRAMMGCLDETRTLEYGQVFVQISSARRRNLS-D 780
Query: 781 TSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDCVVFPQKGSRPH 840
+ AFNM+ E R+ I+GNV VAKNPCLHPGD+RVLKAVN+P LYH+VDCVVFPQKGSRPH
Sbjct: 781 SFAFNMSGPEHRLVIEGNVTVAKNPCLHPGDVRVLKAVNIPWLYHLVDCVVFPQKGSRPH 840
Query: 841 PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN 900
PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPP QLDRDVT ED+QEYFVNYMVN
Sbjct: 841 PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPIQLDRDVTTEDIQEYFVNYMVN 900
Query: 901 DSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF 960
DSLGIIANAHTAFADKE FKAR SPC+ELAKLFSIAVDFPKTGVPAIIPP LYVKEFPDF
Sbjct: 901 DSLGIIANAHTAFADKEPFKARGSPCVELAKLFSIAVDFPKTGVPAIIPPHLYVKEFPDF 960
Query: 961 MDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA 1020
M+KPDKPSYESKNVIGKLFRAVKDIAPT S+I FTR+VA QCYDSDME+EG+EDYV DA
Sbjct: 961 MEKPDKPSYESKNVIGKLFRAVKDIAPTLSYIRSFTRDVAMQCYDSDMEVEGFEDYVGDA 1020
Query: 1021 IYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART 1080
Y+K+NYDYKLGNLLDYYGIKSEAEILSG+IM MSKSFT++RDAEAINLA+RSLRKEART
Sbjct: 1021 FYHKSNYDYKLGNLLDYYGIKSEAEILSGSIMRMSKSFTRRRDAEAINLAVRSLRKEART 1080
Query: 1081 WFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL 1140
WFN RE G +SDS DDLFAKASAWYHVTYH SYWG YNE+M RDHYLSFPWCVYDKL
Sbjct: 1081 WFNAREGGSNSDS----DDLFAKASAWYHVTYHHSYWGCYNEEMKRDHYLSFPWCVYDKL 1129
Query: 1141 MQIKENNVRRKQRASRLAALDSRFRNALNLG 1172
MQIKE N+R+++RA RLA LD RF +ALNLG
Sbjct: 1141 MQIKEKNLRKRERALRLATLD-RFGHALNLG 1129
BLAST of MS020748 vs. NCBI nr
Match:
XP_022943173.1 (RNA-dependent RNA polymerase 1-like isoform X2 [Cucurbita moschata] >XP_022943174.1 RNA-dependent RNA polymerase 1-like isoform X2 [Cucurbita moschata] >XP_022943175.1 RNA-dependent RNA polymerase 1-like isoform X2 [Cucurbita moschata])
HSP 1 Score: 1892.5 bits (4901), Expect = 0.0e+00
Identity = 935/1170 (79.91%), Postives = 1031/1170 (88.12%), Query Frame = 0
Query: 1 MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60
MGKTVHISGFP+ TAD+V+S+LE RTG+GTVYA+K+R PK+GGSR+YA VQF G+ +AE
Sbjct: 1 MGKTVHISGFPSHATADSVKSFLELRTGLGTVYAIKVRPPKRGGSRVYAIVQFIGAAQAE 60
Query: 61 LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120
LIISLAN QRLWYG+SYLKAR ADVDI+PKPRTY+HTLEDLTLCFGCQVSSEKF VLW
Sbjct: 61 LIISLAN---QRLWYGSSYLKARAADVDIVPKPRTYVHTLEDLTLCFGCQVSSEKFHVLW 120
Query: 121 EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLGSN 180
EG LVTFG MRKMNF L HNFVEYRF+LSYENIW+I+LH +HQSMK L+I
Sbjct: 121 EGDVELVTFGVEMRKMNFHLIHNFVEYRFQLSYENIWQIQLHRLQHQSMKCLLI------ 180
Query: 181 SFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVPDD 240
QLYGAPRIYKKV SSG ++FDDPL N+FKEVPDD
Sbjct: 181 ------------------------QLYGAPRIYKKVAPSSG--QIFDDPLFNFFKEVPDD 240
Query: 241 QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSCSA 300
QWVR+ DFTPS+ +GQSSSLCLKL N R+LPNF ++FAYY+E EDEF+ VDGGN FSCS
Sbjct: 241 QWVRTADFTPSNSVGQSSSLCLKLPNGRQLPNFRRHFAYYEEFEDEFKLVDGGNSFSCSD 300
Query: 301 DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSRIKIEFVEHALDR 360
DLVP+VDSRP++ LPY+IIFK+N LVQ+GCI+GP+LDT FY LVDPSRI++E+VE+AL++
Sbjct: 301 DLVPVVDSRPNVLLPYEIIFKVNVLVQNGCIAGPSLDTGFYRLVDPSRIRVEYVENALEK 360
Query: 361 LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420
LFHLKEC Y+P S+LTEQYKKY + P NSPAI+LD GLVYVRRVQITPCKVYFCGPEVNV
Sbjct: 361 LFHLKECCYEPSSFLTEQYKKYAKHPSNSPAIALDDGLVYVRRVQITPCKVYFCGPEVNV 420
Query: 421 SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI 480
SNRVLRHFSRD+DNFLRVSFVDEEWDKMRS DLLPR +S+I++ KT+IY+RILSVLKNGI
Sbjct: 421 SNRVLRHFSRDLDNFLRVSFVDEEWDKMRSTDLLPRMASSIENGKTDIYRRILSVLKNGI 480
Query: 481 VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPAKYAARLGQSF 540
IG K FKFLAFSSSQLR+NSLWMFA+RPGLDAA IR WMGDFR IKNPAKYAARLGQSF
Sbjct: 481 AIGDKKFKFLAFSSSQLRENSLWMFASRPGLDAAVIREWMGDFRHIKNPAKYAARLGQSF 540
Query: 541 GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF 600
GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAK+V++KC L AFTPSAF
Sbjct: 541 GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKRVASKCVL-AFTPSAF 600
Query: 601 QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660
QIRYGGYKGVVAVDP SS KLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST
Sbjct: 601 QIRYGGYKGVVAVDPRSSVKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660
Query: 661 LGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGYKPNVEPY 720
LGV+DEVFEKKQ EA++QLDAILTDPLKAQEAL+LM+PGENTN+LKEMLKCGYKP+VEPY
Sbjct: 661 LGVKDEVFEKKQSEALEQLDAILTDPLKAQEALELMSPGENTNILKEMLKCGYKPDVEPY 720
Query: 721 LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTKQSILSVT 780
LSMMLQ FR SKLL+LRTKSR+FIPNGRAMMGCLDETG+LEYGQVFVQ+S + +S
Sbjct: 721 LSMMLQTFRESKLLELRTKSRIFIPNGRAMMGCLDETGTLEYGQVFVQISSVRHRHIS-D 780
Query: 781 TSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDCVVFPQKGSRPH 840
TSAF+M+ SE + I GNV VAKNPCLHPGD+RVL AVNVP LYHMVDCVVFPQKGSRPH
Sbjct: 781 TSAFDMSGSEDHLVIVGNVTVAKNPCLHPGDVRVLNAVNVPLLYHMVDCVVFPQKGSRPH 840
Query: 841 PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN 900
PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPP QLDRDVTIEDVQEYFVNYMVN
Sbjct: 841 PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPIQLDRDVTIEDVQEYFVNYMVN 900
Query: 901 DSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF 960
DSLGIIANAHTAFADKE FKARS+PCIELAKLFSIAVDFPKTGVPAIIP L VKEFPDF
Sbjct: 901 DSLGIIANAHTAFADKEPFKARSAPCIELAKLFSIAVDFPKTGVPAIIPSHLNVKEFPDF 960
Query: 961 MDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA 1020
M+KPD+PSYESKNVIGKLFRAVK IAP S+I FTR+VA +CYDSDME EG+EDYV+DA
Sbjct: 961 MEKPDRPSYESKNVIGKLFRAVKGIAPNISYIRSFTRDVAMRCYDSDMETEGFEDYVEDA 1020
Query: 1021 IYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART 1080
IY+K YDYKLGNLLDYYGIKSE+EI SGNIM MSKSFT++RDAEAINLA RSLRKEART
Sbjct: 1021 IYHKTIYDYKLGNLLDYYGIKSESEIFSGNIMRMSKSFTRRRDAEAINLAARSLRKEART 1080
Query: 1081 WFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL 1140
WFN +ESGPDS G DDLFAKASAWYHVTYH SYWGSYN+ M RDHYLSFPWCVY KL
Sbjct: 1081 WFNTKESGPDS----GSDDLFAKASAWYHVTYHHSYWGSYNDGMKRDHYLSFPWCVYHKL 1128
Query: 1141 MQIKENNVRRKQRASRLAALDSRFRNALNL 1171
MQ+KENN+RR+++ASRLA LD RF LNL
Sbjct: 1141 MQVKENNLRRREKASRLATLD-RFSRVLNL 1128
BLAST of MS020748 vs. NCBI nr
Match:
XP_022943172.1 (RNA-dependent RNA polymerase 1-like isoform X1 [Cucurbita moschata])
HSP 1 Score: 1892.5 bits (4901), Expect = 0.0e+00
Identity = 935/1170 (79.91%), Postives = 1031/1170 (88.12%), Query Frame = 0
Query: 1 MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60
MGKTVHISGFP+ TAD+V+S+LE RTG+GTVYA+K+R PK+GGSR+YA VQF G+ +AE
Sbjct: 15 MGKTVHISGFPSHATADSVKSFLELRTGLGTVYAIKVRPPKRGGSRVYAIVQFIGAAQAE 74
Query: 61 LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120
LIISLAN QRLWYG+SYLKAR ADVDI+PKPRTY+HTLEDLTLCFGCQVSSEKF VLW
Sbjct: 75 LIISLAN---QRLWYGSSYLKARAADVDIVPKPRTYVHTLEDLTLCFGCQVSSEKFHVLW 134
Query: 121 EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLGSN 180
EG LVTFG MRKMNF L HNFVEYRF+LSYENIW+I+LH +HQSMK L+I
Sbjct: 135 EGDVELVTFGVEMRKMNFHLIHNFVEYRFQLSYENIWQIQLHRLQHQSMKCLLI------ 194
Query: 181 SFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVPDD 240
QLYGAPRIYKKV SSG ++FDDPL N+FKEVPDD
Sbjct: 195 ------------------------QLYGAPRIYKKVAPSSG--QIFDDPLFNFFKEVPDD 254
Query: 241 QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSCSA 300
QWVR+ DFTPS+ +GQSSSLCLKL N R+LPNF ++FAYY+E EDEF+ VDGGN FSCS
Sbjct: 255 QWVRTADFTPSNSVGQSSSLCLKLPNGRQLPNFRRHFAYYEEFEDEFKLVDGGNSFSCSD 314
Query: 301 DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSRIKIEFVEHALDR 360
DLVP+VDSRP++ LPY+IIFK+N LVQ+GCI+GP+LDT FY LVDPSRI++E+VE+AL++
Sbjct: 315 DLVPVVDSRPNVLLPYEIIFKVNVLVQNGCIAGPSLDTGFYRLVDPSRIRVEYVENALEK 374
Query: 361 LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420
LFHLKEC Y+P S+LTEQYKKY + P NSPAI+LD GLVYVRRVQITPCKVYFCGPEVNV
Sbjct: 375 LFHLKECCYEPSSFLTEQYKKYAKHPSNSPAIALDDGLVYVRRVQITPCKVYFCGPEVNV 434
Query: 421 SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI 480
SNRVLRHFSRD+DNFLRVSFVDEEWDKMRS DLLPR +S+I++ KT+IY+RILSVLKNGI
Sbjct: 435 SNRVLRHFSRDLDNFLRVSFVDEEWDKMRSTDLLPRMASSIENGKTDIYRRILSVLKNGI 494
Query: 481 VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPAKYAARLGQSF 540
IG K FKFLAFSSSQLR+NSLWMFA+RPGLDAA IR WMGDFR IKNPAKYAARLGQSF
Sbjct: 495 AIGDKKFKFLAFSSSQLRENSLWMFASRPGLDAAVIREWMGDFRHIKNPAKYAARLGQSF 554
Query: 541 GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF 600
GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAK+V++KC L AFTPSAF
Sbjct: 555 GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKRVASKCVL-AFTPSAF 614
Query: 601 QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660
QIRYGGYKGVVAVDP SS KLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST
Sbjct: 615 QIRYGGYKGVVAVDPRSSVKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 674
Query: 661 LGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGYKPNVEPY 720
LGV+DEVFEKKQ EA++QLDAILTDPLKAQEAL+LM+PGENTN+LKEMLKCGYKP+VEPY
Sbjct: 675 LGVKDEVFEKKQSEALEQLDAILTDPLKAQEALELMSPGENTNILKEMLKCGYKPDVEPY 734
Query: 721 LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTKQSILSVT 780
LSMMLQ FR SKLL+LRTKSR+FIPNGRAMMGCLDETG+LEYGQVFVQ+S + +S
Sbjct: 735 LSMMLQTFRESKLLELRTKSRIFIPNGRAMMGCLDETGTLEYGQVFVQISSVRHRHIS-D 794
Query: 781 TSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDCVVFPQKGSRPH 840
TSAF+M+ SE + I GNV VAKNPCLHPGD+RVL AVNVP LYHMVDCVVFPQKGSRPH
Sbjct: 795 TSAFDMSGSEDHLVIVGNVTVAKNPCLHPGDVRVLNAVNVPLLYHMVDCVVFPQKGSRPH 854
Query: 841 PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN 900
PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPP QLDRDVTIEDVQEYFVNYMVN
Sbjct: 855 PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPIQLDRDVTIEDVQEYFVNYMVN 914
Query: 901 DSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF 960
DSLGIIANAHTAFADKE FKARS+PCIELAKLFSIAVDFPKTGVPAIIP L VKEFPDF
Sbjct: 915 DSLGIIANAHTAFADKEPFKARSAPCIELAKLFSIAVDFPKTGVPAIIPSHLNVKEFPDF 974
Query: 961 MDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA 1020
M+KPD+PSYESKNVIGKLFRAVK IAP S+I FTR+VA +CYDSDME EG+EDYV+DA
Sbjct: 975 MEKPDRPSYESKNVIGKLFRAVKGIAPNISYIRSFTRDVAMRCYDSDMETEGFEDYVEDA 1034
Query: 1021 IYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART 1080
IY+K YDYKLGNLLDYYGIKSE+EI SGNIM MSKSFT++RDAEAINLA RSLRKEART
Sbjct: 1035 IYHKTIYDYKLGNLLDYYGIKSESEIFSGNIMRMSKSFTRRRDAEAINLAARSLRKEART 1094
Query: 1081 WFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL 1140
WFN +ESGPDS G DDLFAKASAWYHVTYH SYWGSYN+ M RDHYLSFPWCVY KL
Sbjct: 1095 WFNTKESGPDS----GSDDLFAKASAWYHVTYHHSYWGSYNDGMKRDHYLSFPWCVYHKL 1142
Query: 1141 MQIKENNVRRKQRASRLAALDSRFRNALNL 1171
MQ+KENN+RR+++ASRLA LD RF LNL
Sbjct: 1155 MQVKENNLRRREKASRLATLD-RFSRVLNL 1142
BLAST of MS020748 vs. ExPASy Swiss-Prot
Match:
Q9LQV2 (RNA-dependent RNA polymerase 1 OS=Arabidopsis thaliana OX=3702 GN=RDR1 PE=2 SV=1)
HSP 1 Score: 1407.5 bits (3642), Expect = 0.0e+00
Identity = 711/1168 (60.87%), Postives = 871/1168 (74.57%), Query Frame = 0
Query: 1 MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60
MGKT+ + GFP GV+A+ V+ +LE TG GTVYA+K+R+PKKGG R+YA VQFT R
Sbjct: 1 MGKTIQVFGFPNGVSAEEVKKFLERLTGSGTVYAIKVRQPKKGGPRVYAIVQFTSERHTR 60
Query: 61 LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120
LII+ A +RL+YG SYLKA E + DI+PKPR +HT+ L + FGCQVS++KF LW
Sbjct: 61 LIITAA---AERLYYGRSYLKAFEVEQDIVPKPRASLHTISGLKMFFGCQVSTKKFLTLW 120
Query: 121 EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLGSN 180
+DV V+FG GMRK++F S +YR ELSYENIW+I+LH + +S K+LVIQV+
Sbjct: 121 SAQDVCVSFGIGMRKLHFSFSWYQKDYRLELSYENIWQIDLHSPQGRSSKFLVIQVI--- 180
Query: 181 SFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVPDD 240
GAP+I++K + +F +++++ + D+
Sbjct: 181 ---------------------------GAPKIFEKEDQP--INLLFG--IMDFYSDGSDE 240
Query: 241 QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSCSA 300
QW+R+TDFT SSCIGQS++ CL+L +P+F +NFA Y E ++ G+ +S +A
Sbjct: 241 QWIRTTDFTSSSCIGQSTAFCLELPVHLNVPDFRENFANYAEHRASSFLIESGSSYSSNA 300
Query: 301 D-LVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSRIKIEFVEHALD 360
+ LVP+VD P LP++I+FK+N LVQ+ C+SGPALD FY L++ + ++H L+
Sbjct: 301 NTLVPVVDPPPGFSLPFEILFKLNTLVQNACLSGPALDLDFYRLLNQKKYDRALIDHCLE 360
Query: 361 RLFHLKECEYKPYSWLTEQYKKYLRRP--PNSPAISLDAGLVYVRRVQITPCKVYFCGPE 420
+LFHL EC Y+P WL ++YKK++ + P SP ISLD GLVY+ RVQ+TP +VYF GPE
Sbjct: 361 KLFHLGECCYEPAHWLRDEYKKWISKGKLPLSPTISLDDGLVYMYRVQVTPARVYFSGPE 420
Query: 421 VNVSNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLK 480
VNVSNRVLRH+S+ I+NFLRVSFVDE+ +K+RS+DL PR+S+ R+T++Y RI SVL+
Sbjct: 421 VNVSNRVLRHYSKYINNFLRVSFVDEDLEKVRSMDLSPRSST---QRRTKLYDRIYSVLR 480
Query: 481 NGIVIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPAKYAARLG 540
+GIVIG K F+FLAFSSSQLR+NS WMFA + AA IRAWMGDF I+N AKYAARLG
Sbjct: 481 DGIVIGDKKFEFLAFSSSQLRENSAWMFAPIDRITAAHIRAWMGDFDHIRNVAKYAARLG 540
Query: 541 QSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTP 600
QSF SS ETL+V E E+IPD+E+ +YVFSDGIGKIS++FA+KV+ KCGL F+P
Sbjct: 541 QSFSSSRETLNVRSDEIEVIPDVEIISLGTRYVFSDGIGKISAEFARKVARKCGLTEFSP 600
Query: 601 SAFQIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITL 660
SAFQIRYGGYKGVVAVDP SSKKLSLRKSM KFES+NTKLDVL +SKYQPC++NRQLITL
Sbjct: 601 SAFQIRYGGYKGVVAVDPNSSKKLSLRKSMSKFESENTKLDVLAWSKYQPCYMNRQLITL 660
Query: 661 LSTLGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGYKPNV 720
LSTLGV D VFEKKQ E V +LDAILT PL+A EAL LMAPGENTN+LK ++ CGYKP+
Sbjct: 661 LSTLGVTDSVFEKKQREVVDRLDAILTHPLEAHEALGLMAPGENTNILKALILCGYKPDA 720
Query: 721 EPYLSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTKQSIL 780
EP+LSMMLQ FRASKLL+LRTK+R+FI GR+MMGCLDET +LEYGQV VQ S +
Sbjct: 721 EPFLSMMLQNFRASKLLELRTKTRIFISGGRSMMGCLDETRTLEYGQVVVQYSDPMR--- 780
Query: 781 SVTTSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDCVVFPQKGS 840
+R I G VVVAKNPCLHPGD+RVL+AVNVP L HMVDCVVFPQKG
Sbjct: 781 -----------PGRRFIITGPVVVAKNPCLHPGDVRVLQAVNVPALNHMVDCVVFPQKGL 840
Query: 841 RPHPNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNY 900
RPHPNECSGSDLDGDIYFVCWD EL+PPR PMDYTP P LD DVTIE+V+EYF NY
Sbjct: 841 RPHPNECSGSDLDGDIYFVCWDQELVPPRTSEPMDYTPEPTQILDHDVTIEEVEEYFANY 900
Query: 901 MVNDSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIPPELYVKEF 960
+VNDSLGIIANAHTAFADKE KA S PCIELAK FS AVDFPKTGV A+IP LYVKE+
Sbjct: 901 IVNDSLGIIANAHTAFADKEPLKAFSDPCIELAKKFSTAVDFPKTGVAAVIPQHLYVKEY 960
Query: 961 PDFMDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYV 1020
PDFM+KPDKP+YESKNVIGKLFR VK+ AP I FT +VA + YD DME++G+E+YV
Sbjct: 961 PDFMEKPDKPTYESKNVIGKLFREVKERAPPLISIKSFTLDVASKSYDKDMEVDGFEEYV 1020
Query: 1021 DDAIYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKE 1080
D+A Y K NYD+KLGNL+DYYGIK+EAEILSG IM MSKSFTK+RDAE+I A+R+LRKE
Sbjct: 1021 DEAFYQKANYDFKLGNLMDYYGIKTEAEILSGGIMRMSKSFTKRRDAESIGRAVRALRKE 1080
Query: 1081 ARTWFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVY 1140
+ FN E + AKASAWYHVTYH SYWG YNE +NRDH+LSF WCVY
Sbjct: 1081 TLSLFNASEEEENES---------AKASAWYHVTYHSSYWGLYNEGLNRDHFLSFAWCVY 1105
Query: 1141 DKLMQIKENNVRRKQRASRLAALDSRFR 1166
DKL++IK+ N+ R+QR L LD R
Sbjct: 1141 DKLVRIKKTNLGRRQRQETLERLDHVLR 1105
BLAST of MS020748 vs. ExPASy Swiss-Prot
Match:
Q0DXS3 (Probable RNA-dependent RNA polymerase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=RDR1 PE=2 SV=2)
HSP 1 Score: 987.3 bits (2551), Expect = 1.5e-286
Identity = 497/744 (66.80%), Postives = 592/744 (79.57%), Query Frame = 0
Query: 411 VYFCGPEVNVSNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYK 470
VYF GPE+NVSNRV+R+FS DI+NFLR+SFVDE+ +K+R+ DL PR++S +T +YK
Sbjct: 9 VYFYGPEINVSNRVVRNFSSDIENFLRISFVDEDCEKLRATDLSPRSASGHDANRTALYK 68
Query: 471 RILSVLKNGIVIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPA 530
R+LSVL +GI IGGK F+FLAFSSSQLRDNS WMFA+R GL A+DIR WMGDFR I+N A
Sbjct: 69 RVLSVLSDGITIGGKNFEFLAFSSSQLRDNSAWMFASRQGLAASDIRTWMGDFRNIRNVA 128
Query: 531 KYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKC 590
KYAARLGQSF SSTETL V ++E E I DI+ ++VFSDGIGKISS FA +V+ KC
Sbjct: 129 KYAARLGQSFSSSTETLKVQKYEVEEISDIK---NGTQHVFSDGIGKISSAFANEVAMKC 188
Query: 591 GLKAFTPSAFQIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFL 650
LK F PSAFQIRYGGYKGVVAVDP S KLSLRKSM KF+SDN +DVL YSKYQP FL
Sbjct: 189 NLKRFAPSAFQIRYGGYKGVVAVDPTSRWKLSLRKSMLKFQSDNITVDVLAYSKYQPGFL 248
Query: 651 NRQLITLLSTLGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLK 710
NRQLITLLSTLGVRD VFE+KQ EAV QL+ ++TDP A EA++LM GE TN +KE+L
Sbjct: 249 NRQLITLLSTLGVRDSVFEQKQEEAVNQLNKMVTDPQAAIEAIELMPMGEITNAVKELLL 308
Query: 711 CGYKPNVEPYLSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVS 770
CGY+P+ EPYLSM+LQ FRASKLL+L+TKSR+ IP GRAMMGCLDET +L+YGQVF++
Sbjct: 309 CGYQPDDEPYLSMLLQTFRASKLLELKTKSRILIPKGRAMMGCLDETRTLKYGQVFIR-- 368
Query: 771 GTKQSILSVTTSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDCV 830
TS N N R + G VV+AKNPCLHPGDIR+L AV+VP L+HM +CV
Sbjct: 369 ---------ATSGVNDN---DRFTVTGKVVIAKNPCLHPGDIRILHAVDVPVLHHMFNCV 428
Query: 831 VFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDV 890
VFPQ+G RPHPNECSGSDLDGDIYFV WD LIPPR + PMDYTPAP LD DVTIE+V
Sbjct: 429 VFPQQGPRPHPNECSGSDLDGDIYFVSWDPSLIPPRMVTPMDYTPAPTETLDHDVTIEEV 488
Query: 891 QEYFVNYMVNDSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIPP 950
+EYF NY+VN+SLG+IANAH FADKE KA SSPCIELAKLFSIAVDFPKTGVPA+IPP
Sbjct: 489 EEYFTNYIVNESLGMIANAHVVFADKEDLKAESSPCIELAKLFSIAVDFPKTGVPALIPP 548
Query: 951 ELYVKEFPDFMDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEI 1010
EL+VKE+PDFM+K DK +YESK VIGKL+R +K P HI FTREVAR+ YD+DM +
Sbjct: 549 ELHVKEYPDFMEKLDKVTYESKGVIGKLYREIKKHTP---HIKHFTREVARRSYDTDMIV 608
Query: 1011 EGYEDYVDDAIYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLA 1070
+GYEDY+ +A+ K+ YD+KLGNL+D+YGIKSEAEI+SG I+ M+K+FTKK DA+AI LA
Sbjct: 609 DGYEDYITEAMALKDEYDFKLGNLMDHYGIKSEAEIISGCILKMAKNFTKKSDADAIRLA 668
Query: 1071 IRSLRKEARTWFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYL 1130
+RSLRKEAR+ F+E D+ G D AKASAWYHVTYHP +WG YNE R H++
Sbjct: 669 VRSLRKEARSRFSEMSL---DDNGHGHDASEAKASAWYHVTYHPEFWGCYNEGYERPHFI 728
Query: 1131 SFPWCVYDKLMQIKENN--VRRKQ 1153
SFPWC+Y+KL++IK+ VR+ Q
Sbjct: 729 SFPWCIYEKLLRIKQRRKFVRKMQ 729
BLAST of MS020748 vs. ExPASy Swiss-Prot
Match:
O82504 (RNA-dependent RNA polymerase 2 OS=Arabidopsis thaliana OX=3702 GN=RDR2 PE=1 SV=1)
HSP 1 Score: 758.4 bits (1957), Expect = 1.2e-217
Identity = 466/1181 (39.46%), Postives = 678/1181 (57.41%), Query Frame = 0
Query: 4 TVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGS-RLYAFVQFTGSREAELI 63
TV IS P + AD + +LE G TV+AL+I + R +A VQFT
Sbjct: 11 TVKISNVPQTIVADELLRFLELHLGEDTVFALEIPTTRDNWKPRDFARVQFTTLEVKSRA 70
Query: 64 ISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEK-FCVL-- 123
L++Q +L + T L+ EA DIIP+P L+D+ L G S EK FC L
Sbjct: 71 QLLSSQ--SKLLFKTHNLRLSEAYDDIIPRPVDPRKRLDDIVLTVGFPESDEKRFCALEK 130
Query: 124 WEG-RDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLG 183
W+G R ++T R++ F + + Y+ E+ +E+I ++ L V G
Sbjct: 131 WDGVRCWILT---EKRRVEFWVWESGDCYKIEVRFEDI------------IETLSCCVNG 190
Query: 184 SNSFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVP 243
S ++ +L LK YG P+++K+V ++ F + KE
Sbjct: 191 DAS--EIDAFLLKLK-------------YG-PKVFKRVTVHIATK--FKSDRYRFCKEDF 250
Query: 244 DDQWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSC 303
D W+R+TDF+ S IG S+ CL++HN + + YY+E +VD G F+
Sbjct: 251 DFMWIRTTDFSGSKSIGTSTCFCLEVHNGSTMLDIFSGLPYYREDTLSLTYVD-GKTFAS 310
Query: 304 SADLVPMVDSR-PDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSR-IKIEFVEH 363
+A +VP++++ + PY+I+F++NALV IS S L+ R + +E
Sbjct: 311 AAQIVPLLNAAILGLEFPYEILFQLNALVHAQKIS--LFAASDMELIKILRGMSLETALV 370
Query: 364 ALDRLFHLKECEYKPYSWLTEQYKKYLRRPPNSPAIS----LDAGLVYVRRVQITPCKVY 423
L +L Y P ++ Q + +++ +SPA + + ++ +R +TP K+Y
Sbjct: 371 ILKKLHQQSSICYDPVFFVKTQMQSVVKKMKHSPASAYKRLTEQNIMSCQRAYVTPSKIY 430
Query: 424 FCGPEVNVSNRVLRHFSRDIDNFLRVSFVDEEWDKM--RSIDLLPRTSSTIKDRKTEIYK 483
GPE+ +N V+++F+ + +F+RV+FV+E+W K+ ++ + + +K +T IY
Sbjct: 431 LLGPELETANYVVKNFAEHVSDFMRVTFVEEDWSKLPANALSVNSKEGYFVKPSRTNIYN 490
Query: 484 RILSVLKNGIVIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPA 543
R+LS+L GI +G K F+FLAFS+SQLR NS+WMFA+ + A DIR WMG FR+I++ +
Sbjct: 491 RVLSILGEGITVGPKRFEFLAFSASQLRGNSVWMFASNEKVKAEDIREWMGCFRKIRSIS 550
Query: 544 KYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKC 603
K AAR+GQ F +S +TL V + E IPDIEV Y FSDGIGKIS FAK+V+ KC
Sbjct: 551 KCAARMGQLFSASRQTLIVRAQDVEQIPDIEVTTDGADYCFSDGIGKISLAFAKQVAQKC 610
Query: 604 GLKAFTPSAFQIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFL 663
GL + PSAFQIRYGGYKGV+AVD S +KLSLR SM KF+S+N L+V +++ PCFL
Sbjct: 611 GL-SHVPSAFQIRYGGYKGVIAVDRSSFRKLSLRDSMLKFDSNNRMLNVTRWTESMPCFL 670
Query: 664 NRQLITLLSTLGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLK 723
NR++I LLSTLG+ D +FE Q + L +L D A L ++ + N+L +ML
Sbjct: 671 NREIICLLSTLGIEDAMFEAMQAVHLSMLGNMLEDRDAALNVLQKLSGENSKNLLVKMLL 730
Query: 724 CGYKPNVEPYLSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVS 783
GY P+ EPYLSMML+ S+L +L+++ R+ +P GR ++GC+DE G LEYGQV+V+V+
Sbjct: 731 QGYAPSSEPYLSMMLRVHHESQLSELKSRCRILVPKGRILIGCMDEMGILEYGQVYVRVT 790
Query: 784 GTKQSILSVTTSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYH--MVD 843
TK + S S F E V I G VVV KNPCLHPGDIRVL A+ +D
Sbjct: 791 LTKAELKSRDQSYFRKIDEETSVVI-GKVVVTKNPCLHPGDIRVLDAIYEVHFEEKGYLD 850
Query: 844 CVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIE 903
C++FPQKG RPHPNECSG DLDGD +FV WD ++IP PPMDY + P +D DVT+E
Sbjct: 851 CIIFPQKGERPHPNECSGGDLDGDQFFVSWDEKIIPSEMDPPMDYAGSRPRLMDHDVTLE 910
Query: 904 DVQEYFVNYMVNDSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAII 963
++ ++FV+YM++D+LG+I+ AH AD++ KARS C+ELA L S AVDF KTG PA +
Sbjct: 911 EIHKFFVDYMISDTLGVISTAHLVHADRDPEKARSQKCLELANLHSRAVDFAKTGAPAEM 970
Query: 964 PPELYVKEFPDFMDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDM 1023
P L +EFPDF+++ +KP+Y S++V GKL+RAVK + VA YD +
Sbjct: 971 PYALKPREFPDFLERFEKPTYISESVFGKLYRAVKSSLAQRKPEAESEDTVA---YDVTL 1030
Query: 1024 EIEGYEDYVDDAIYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRD----- 1083
E G+E +++ A +++ Y KL +L+ YYG +E EIL+G + T K RD
Sbjct: 1031 EEAGFESFIETAKAHRDMYGEKLTSLMIYYGAANEEEILTGILKT--KEMYLARDNRRYG 1090
Query: 1084 --AEAINLAIRSLRKEARTWFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYN 1143
+ I L+++ L KEA WF + L ASAWY+VTY+P N
Sbjct: 1091 DMKDRITLSVKDLHKEAMGWFEKSCEDEQQKKKL--------ASAWYYVTYNP------N 1132
Query: 1144 EDMNRDHYLSFPWCVYDKLMQIKENNVRRKQRASRLAALDS 1163
+ +LSFPW V D L+ IK N +R+ + + L S
Sbjct: 1151 HRDEKLTFLSFPWIVGDVLLDIKAENAQRQSVEEKTSGLVS 1132
BLAST of MS020748 vs. ExPASy Swiss-Prot
Match:
Q7XM31 (Probable RNA-dependent RNA polymerase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=RDR2 PE=2 SV=1)
HSP 1 Score: 720.7 bits (1859), Expect = 2.7e-206
Identity = 434/1173 (37.00%), Postives = 653/1173 (55.67%), Query Frame = 0
Query: 4 TVHISGFPAGVTADAVRSYLEGRTGV-GTVYALKIREPKKGG-SRLYAFVQFTGSREAEL 63
++ +S P A + ++ + V G +A +I +G SR + VQF + A
Sbjct: 11 SLRVSNIPPSAVAAELLAFFDSAVAVAGGAFACEIAAAHRGWLSRGHGTVQFGSAAAAAA 70
Query: 64 IISLANQ-RIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKF--CV 123
LA+ R+ R + + L + VD++P+ L G +V+ F
Sbjct: 71 AAGLASSGRLPR--FLGALLSVSPSPVDLLPRASDLSLRAAGAGLVVGDRVAERVFEAAD 130
Query: 124 LWEGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLG 183
W+G V G R+++ L H+ Y+ E+ +E++ + + +G
Sbjct: 131 AWDG--VRAEVIPGKRRVDLYLEHDSQRYKLEVLFEDM----------KDCLGCTLDGMG 190
Query: 184 SNSFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVP 243
AIL+ QL APRI+ + + + R DD + KE
Sbjct: 191 ---------AILL-------------QLNYAPRIHTAISGPAVNSRFMDD-RFHACKEDA 250
Query: 244 DDQWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSC 303
WVR+ DFTP+ G+ S+L LKL + + ++ + + ++ +G
Sbjct: 251 KFSWVRALDFTPNYSFGRCSTLVLKLGKSALVSDILKSLPFSGNLGE--LTMNSMDGVGA 310
Query: 304 SADLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSRIKIEFVEHAL 363
S+++VP+V D +PY+++F++N+L+ G I ++ + + + ++
Sbjct: 311 SSNVVPLVHCPRDYSVPYEVLFRLNSLMHMGKIVAKHVNADLFKAL--QELPVDVSRRIF 370
Query: 364 DRLFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDA------GLVYVRRVQITPCKVY 423
+++ L+ Y P ++ Q + Y + ++ +S + L+ RV ITP K++
Sbjct: 371 EKMHKLESTCYGPLQFI--QQEAYSMKRSHNVLLSNEGEGEGERKLMKCYRVNITPSKIF 430
Query: 424 FCGPEVNVSNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTI--KDRKTEIYK 483
GPE V+N V++H S +F+RV+FVDE+W K+ S + R K KT +Y
Sbjct: 431 CFGPEEEVTNYVVKHHSAYASDFVRVTFVDEDWSKLSSNAISARIEQGFFSKPFKTGLYY 490
Query: 484 RILSVLKNGIVIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPA 543
RILS+LK G IG K F+FLAFS+SQLR NS+WMFA+ L+A IR WMG F I++ +
Sbjct: 491 RILSILKKGFSIGPKNFEFLAFSASQLRGNSVWMFASNASLNAGGIRRWMGHFENIRSVS 550
Query: 544 KYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKC 603
K AAR+GQ F SS +T V R + E+IPDIE+ KY+FSDGIGKIS FAK+V+
Sbjct: 551 KCAARMGQLFSSSRQTFEVLRWDVEVIPDIEITTDGSKYIFSDGIGKISLRFAKRVAHHV 610
Query: 604 GLKAFT-PSAFQIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCF 663
GL PSAFQIRYGGYKGV+A+DP SS LSLR SM KFES++ L++ +SK QPC+
Sbjct: 611 GLDPTNLPSAFQIRYGGYKGVIAIDPMSSIDLSLRPSMKKFESESRMLNITSWSKSQPCY 670
Query: 664 LNRQLITLLSTLGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEML 723
+NR++I+LLSTLG+RDE+F Q + +++ + +LT+ A L + G T +ML
Sbjct: 671 VNREIISLLSTLGIRDEIFVAMQQDEMRETEEMLTNKEVALSVLGKLG-GSETKTAVKML 730
Query: 724 KCGYKPNVEPYLSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQV 783
GY+P+ EPYLSM+L+A + ++L D+RT+ ++ +P GR ++GCLDETG LEYGQV++++
Sbjct: 731 LQGYEPSSEPYLSMILKAHQENRLTDIRTRCKIHVPKGRVLIGCLDETGVLEYGQVYIRI 790
Query: 784 SGTKQSILSVTTSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDC 843
+ + S F N + + G V + KNPCLHPGDIRVL+A+ P L MVDC
Sbjct: 791 TKNSKEQKDSNQSYF-YNDDGKTATVVGKVAITKNPCLHPGDIRVLEAIYDPDLVGMVDC 850
Query: 844 VVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIED 903
+VFPQ+G RPHPNECSG DLDGD+YF+ WD +LIP + PMDYT P +D VT+E+
Sbjct: 851 LVFPQRGERPHPNECSGGDLDGDLYFITWDDKLIPEKVDTPMDYTATRPRIMDHVVTLEE 910
Query: 904 VQEYFVNYMVNDSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIP 963
+Q++FV+YM+NDSLG I+ AH AD+ KARS C++LA L S+AVDF KTG PA +P
Sbjct: 911 IQKHFVDYMINDSLGAISTAHLIHADRSPLKARSPECLQLATLHSMAVDFAKTGAPAEMP 970
Query: 964 PELYVKEFPDFMDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVAR--QCYDSD 1023
L +E+PDFM++ +KP Y S V+GKL+R+ + + A+ YD D
Sbjct: 971 RTLRPREYPDFMERWEKPMYISNGVLGKLYRSAMGHMEKSGDSGALSSSSAQPSPTYDPD 1030
Query: 1024 MEIEGYEDYVDDAIYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRD---- 1083
+E+ G ++++ A Y Y+ KL L++YY + E EIL+GNI +K KRD
Sbjct: 1031 LEVPGSDEFLQAAEEYYELYEEKLTTLMNYYRAELEDEILTGNI--RNKMLYLKRDNKRY 1090
Query: 1084 ---AEAINLAIRSLRKEARTWFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSY 1143
+ I A+ +L +EAR W D+ + ASAWY VTYHP
Sbjct: 1091 FEMKDRIVAAVDALHREARGWLLSSRKEEDASRM---------ASAWYRVTYHP------ 1120
Query: 1144 NEDMNRDHYLSFPWCVYDKLMQIKENNVRRKQR 1154
+ + SFPW D L+ IK ++ R++R
Sbjct: 1151 -DRRRGKRFWSFPWIACDNLLAIKASSQLRRRR 1120
BLAST of MS020748 vs. ExPASy Swiss-Prot
Match:
Q8LHH9 (Probable RNA-dependent RNA polymerase SHL2 OS=Oryza sativa subsp. japonica OX=39947 GN=SHL2 PE=2 SV=1)
HSP 1 Score: 593.6 bits (1529), Expect = 4.9e-168
Identity = 378/968 (39.05%), Postives = 543/968 (56.10%), Query Frame = 0
Query: 239 DDQWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFS- 298
DD W+R+TD TPS IG+ + + R P + Y +E VD G G+
Sbjct: 267 DDPWIRTTDITPSGAIGRCGVYRISF-SARFWPKMDRALDYMRER--RVAIVDCGGGWGP 326
Query: 299 ----CSADLVPMVDSRPDIF--------LPYKIIFKINALVQHGCISGPALDTSFYHLVD 358
D + + D+F L + ++F +NALV G I+ L F+ L+
Sbjct: 327 RRGLTVRDELEFGEPMQDVFFCLQHAEGLKFPLLFMVNALVHKGIINQHQLTPEFFSLLG 386
Query: 359 PSRIKIEFVEHALDRLFHLKECEYKPYSWLTEQYKKYLRRPPNSPAI---SLDAGLVYVR 418
S E V A R F + P + KK L R +P + + VR
Sbjct: 387 RSE---ENVNVAALRDFW---GDKFPVFDACGRLKKALNRVARNPKLLCSKVGDDHAEVR 446
Query: 419 RVQITPCKVYFCGPEVNVSNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIK 478
R+ ITP + Y PEV SNRVLRH+ D FLRV+F+DE + + L T+ +K
Sbjct: 447 RLVITPTRAYCLPPEVERSNRVLRHYHEVADRFLRVTFMDEGMQVLNNNVLNSFTAPIVK 506
Query: 479 D-------RKTEIYKRILSVLKNGIVIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAAD 538
D +KT +YKR+ +L G + G+ + FLAFSS+QLRD S W FA
Sbjct: 507 DLMSNFFQQKTTVYKRVRMLLTEGFHMCGRKYSFLAFSSNQLRDKSAWFFAEDRKTTVEA 566
Query: 539 IRAWMGDFRQIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGI 598
IR WMG F KN AK+AAR+GQ F S+ T+++ E + D +V H E Y+FSDGI
Sbjct: 567 IRKWMGRFTS-KNVAKHAARMGQCFSSTYATVTMRPDEVDESFD-DVVHNE--YIFSDGI 626
Query: 599 GKISSDFAKKVSAKCGLKAFTPSAFQIRYGGYKGVVAV--DPYSSKKLSLRKSMCKFESD 658
GKI+ D A +V+ + L PSA+QIR+ G+KGV+AV +L LR SM KFES+
Sbjct: 627 GKITPDLALEVAERLQLTDNPPSAYQIRFAGFKGVIAVWQGHGDGTRLFLRPSMRKFESN 686
Query: 659 NTKLDVLGYSKYQPCFLNRQLITLLSTLGVRDEVFEKKQHEAVKQLDAILTDPLKAQEAL 718
+ L+V+ ++K+QP FLNRQ+I LLS+L V D +F + Q + L+ IL+D A E L
Sbjct: 687 HLVLEVVSWTKFQPGFLNRQIIILLSSLNVPDSIFWQMQETMLSNLNNILSDRDVAFEVL 746
Query: 719 DLMAPGENTNVLKEMLKCGYKPNVEPYLSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGC 778
++ N ML G++P EP+L ML A R+++L DL K+R+F+P GR +MGC
Sbjct: 747 TTSC-ADDGNTAALMLSAGFEPRTEPHLKAMLLAIRSAQLQDLLEKARIFVPKGRWLMGC 806
Query: 779 LDETGSLEYGQVFVQVSGTKQSILSVTTSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIR 838
LDE G LE GQ F++ + + V + ++ + I G VV+AKNPCLHPGD+R
Sbjct: 807 LDELGVLEQGQCFIRATVPSLNSYFVKHGSRFSSTDKNTEVILGTVVIAKNPCLHPGDVR 866
Query: 839 VLKAVNVPQLYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPP--RQIPPM 898
+L+AV+VP+L+H+VDC+VFPQKG RPH NE SGSDLDGD+YFV WD +LIPP + PM
Sbjct: 867 ILEAVDVPELHHLVDCLVFPQKGERPHANEASGSDLDGDLYFVTWDEKLIPPGKKSWNPM 926
Query: 899 DYTPAPPTQLDRDVTIEDVQEYFVNYMVNDSLGIIANAHTAFADKELFKARSSPCIELAK 958
DY+P QL R V+ D+ ++F+ M++++LG I NAH AD + A CI LA+
Sbjct: 927 DYSPPEAKQLPRQVSQHDIIDFFLKNMISENLGRICNAHVVHADLSEYGAMDEKCIHLAE 986
Query: 959 LFSIAVDFPKTGVPAIIPPELYVKEFPDFMDKPDKPSYESKNVIGKLFRAVKDIAPTTSH 1018
L + AVDFPKTG AI+PP L K +PDFM K D SY+S+ ++G+L+R++++ S+
Sbjct: 987 LAATAVDFPKTGKLAIMPPHLKPKVYPDFMGKEDGQSYKSEKILGRLYRSIQE----ASN 1046
Query: 1019 ISLFTREVARQ---CYDSDMEIEGYEDYVDDAIYYKNNYDYKLGNLLDYYGIKSEAEILS 1078
+ ++EV YD D+E+ G D++ A K +YD +L LL Y +++EAE+++
Sbjct: 1047 GDVVSQEVCTPNDLPYDIDLEVPGASDFLASAWQCKCSYDAQLSALLSQYRVRTEAELVT 1106
Query: 1079 GNIMTMSKSFTKKRD--AEAINLAIRSLRKEARTWF-----NERESGPDSDSVLGLDDLF 1138
G+I + K+ +KK+ + + A +LRKE ++ F ++ E G D ++L
Sbjct: 1107 GHITFLVKNSSKKQGDIKDRLKTAYSALRKEFKSTFESIASDQCEIGDDEKNLL----YE 1166
Query: 1139 AKASAWYHVTYHPSYWGSYNEDMNRD-----HYLSFPWCVYDKLMQIK-----ENNVRRK 1160
KASAWY VTYHP + + D LSF W D L +IK + V +
Sbjct: 1167 MKASAWYQVTYHPKWVEKSRGILGPDGEEIPASLSFAWIPVDYLARIKLRCHGKVRVEGQ 1212
BLAST of MS020748 vs. ExPASy TrEMBL
Match:
A0A6J1CYE0 (RNA-dependent RNA polymerase OS=Momordica charantia OX=3673 GN=LOC111015923 PE=3 SV=1)
HSP 1 Score: 2278.4 bits (5903), Expect = 0.0e+00
Identity = 1124/1172 (95.90%), Postives = 1134/1172 (96.76%), Query Frame = 0
Query: 1 MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60
MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE
Sbjct: 1 MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60
Query: 61 LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120
LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW
Sbjct: 61 LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120
Query: 121 EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLGSN 180
EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVI
Sbjct: 121 EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVI------ 180
Query: 181 SFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVPDD 240
QLYGAP IYKKVESSSGSR+VFDDPLLNYFKEVPDD
Sbjct: 181 ------------------------QLYGAPWIYKKVESSSGSRQVFDDPLLNYFKEVPDD 240
Query: 241 QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSCSA 300
QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFR VDGGNGFSCSA
Sbjct: 241 QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRLVDGGNGFSCSA 300
Query: 301 DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSRIKIEFVEHALDR 360
DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDP RIKIEFVEHALDR
Sbjct: 301 DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPRRIKIEFVEHALDR 360
Query: 361 LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420
LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV
Sbjct: 361 LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420
Query: 421 SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI 480
SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI
Sbjct: 421 SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI 480
Query: 481 VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPAKYAARLGQSF 540
VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDA DIRAWMGDFRQIKNPAKYAARLGQSF
Sbjct: 481 VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAEDIRAWMGDFRQIKNPAKYAARLGQSF 540
Query: 541 GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF 600
GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF
Sbjct: 541 GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF 600
Query: 601 QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660
QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST
Sbjct: 601 QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660
Query: 661 LGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGYKPNVEPY 720
LGVRDEVFEKKQ EAV+QLDAILTDPLKAQEAL+LMAPGENTNVLKEMLKCGYKPNVEPY
Sbjct: 661 LGVRDEVFEKKQREAVEQLDAILTDPLKAQEALELMAPGENTNVLKEMLKCGYKPNVEPY 720
Query: 721 LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTKQSILSVT 780
LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSG KQSILSVT
Sbjct: 721 LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGAKQSILSVT 780
Query: 781 TSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDCVVFPQKGSRPH 840
TSAFN++SSEQRVAI+GNVVVAKNPCLHPGD+RVLKAVNVP+LYHMVDCVVFPQKGSRPH
Sbjct: 781 TSAFNISSSEQRVAIEGNVVVAKNPCLHPGDVRVLKAVNVPRLYHMVDCVVFPQKGSRPH 840
Query: 841 PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN 900
PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN
Sbjct: 841 PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN 900
Query: 901 DSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF 960
DSLGIIANAHTAFADKELFKARSSPCI LAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF
Sbjct: 901 DSLGIIANAHTAFADKELFKARSSPCINLAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF 960
Query: 961 MDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA 1020
M+KPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA
Sbjct: 961 MEKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA 1020
Query: 1021 IYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART 1080
IY+KNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART
Sbjct: 1021 IYHKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART 1080
Query: 1081 WFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL 1140
WFNERESGPDSDSV GLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL
Sbjct: 1081 WFNERESGPDSDSVSGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL 1140
Query: 1141 MQIKENNVRRKQRASRLAALDSRFRNALNLGH 1173
MQIKENNVRRKQRASRLAALDSRFRNALNLGH
Sbjct: 1141 MQIKENNVRRKQRASRLAALDSRFRNALNLGH 1142
BLAST of MS020748 vs. ExPASy TrEMBL
Match:
A0A6J1D0H1 (RNA-dependent RNA polymerase OS=Momordica charantia OX=3673 GN=LOC111015923 PE=3 SV=1)
HSP 1 Score: 2191.8 bits (5678), Expect = 0.0e+00
Identity = 1091/1172 (93.09%), Postives = 1101/1172 (93.94%), Query Frame = 0
Query: 1 MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60
MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE
Sbjct: 1 MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60
Query: 61 LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120
LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW
Sbjct: 61 LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120
Query: 121 EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLGSN 180
EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVI
Sbjct: 121 EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVI------ 180
Query: 181 SFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVPDD 240
QLYGAP IYKKVESSSGSR+VFDDPLLNYFKEVPDD
Sbjct: 181 ------------------------QLYGAPWIYKKVESSSGSRQVFDDPLLNYFKEVPDD 240
Query: 241 QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSCSA 300
QW NFAYYKETEDEFR VDGGNGFSCSA
Sbjct: 241 QW---------------------------------NFAYYKETEDEFRLVDGGNGFSCSA 300
Query: 301 DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSRIKIEFVEHALDR 360
DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDP RIKIEFVEHALDR
Sbjct: 301 DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPRRIKIEFVEHALDR 360
Query: 361 LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420
LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV
Sbjct: 361 LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420
Query: 421 SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI 480
SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI
Sbjct: 421 SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI 480
Query: 481 VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPAKYAARLGQSF 540
VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDA DIRAWMGDFRQIKNPAKYAARLGQSF
Sbjct: 481 VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAEDIRAWMGDFRQIKNPAKYAARLGQSF 540
Query: 541 GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF 600
GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF
Sbjct: 541 GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF 600
Query: 601 QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660
QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST
Sbjct: 601 QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660
Query: 661 LGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGYKPNVEPY 720
LGVRDEVFEKKQ EAV+QLDAILTDPLKAQEAL+LMAPGENTNVLKEMLKCGYKPNVEPY
Sbjct: 661 LGVRDEVFEKKQREAVEQLDAILTDPLKAQEALELMAPGENTNVLKEMLKCGYKPNVEPY 720
Query: 721 LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTKQSILSVT 780
LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSG KQSILSVT
Sbjct: 721 LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGAKQSILSVT 780
Query: 781 TSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDCVVFPQKGSRPH 840
TSAFN++SSEQRVAI+GNVVVAKNPCLHPGD+RVLKAVNVP+LYHMVDCVVFPQKGSRPH
Sbjct: 781 TSAFNISSSEQRVAIEGNVVVAKNPCLHPGDVRVLKAVNVPRLYHMVDCVVFPQKGSRPH 840
Query: 841 PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN 900
PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN
Sbjct: 841 PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN 900
Query: 901 DSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF 960
DSLGIIANAHTAFADKELFKARSSPCI LAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF
Sbjct: 901 DSLGIIANAHTAFADKELFKARSSPCINLAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF 960
Query: 961 MDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA 1020
M+KPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA
Sbjct: 961 MEKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA 1020
Query: 1021 IYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART 1080
IY+KNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART
Sbjct: 1021 IYHKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART 1080
Query: 1081 WFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL 1140
WFNERESGPDSDSV GLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL
Sbjct: 1081 WFNERESGPDSDSVSGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL 1109
Query: 1141 MQIKENNVRRKQRASRLAALDSRFRNALNLGH 1173
MQIKENNVRRKQRASRLAALDSRFRNALNLGH
Sbjct: 1141 MQIKENNVRRKQRASRLAALDSRFRNALNLGH 1109
BLAST of MS020748 vs. ExPASy TrEMBL
Match:
A0A6J1FXH5 (RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111447985 PE=3 SV=1)
HSP 1 Score: 1892.5 bits (4901), Expect = 0.0e+00
Identity = 935/1170 (79.91%), Postives = 1031/1170 (88.12%), Query Frame = 0
Query: 1 MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60
MGKTVHISGFP+ TAD+V+S+LE RTG+GTVYA+K+R PK+GGSR+YA VQF G+ +AE
Sbjct: 1 MGKTVHISGFPSHATADSVKSFLELRTGLGTVYAIKVRPPKRGGSRVYAIVQFIGAAQAE 60
Query: 61 LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120
LIISLAN QRLWYG+SYLKAR ADVDI+PKPRTY+HTLEDLTLCFGCQVSSEKF VLW
Sbjct: 61 LIISLAN---QRLWYGSSYLKARAADVDIVPKPRTYVHTLEDLTLCFGCQVSSEKFHVLW 120
Query: 121 EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLGSN 180
EG LVTFG MRKMNF L HNFVEYRF+LSYENIW+I+LH +HQSMK L+I
Sbjct: 121 EGDVELVTFGVEMRKMNFHLIHNFVEYRFQLSYENIWQIQLHRLQHQSMKCLLI------ 180
Query: 181 SFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVPDD 240
QLYGAPRIYKKV SSG ++FDDPL N+FKEVPDD
Sbjct: 181 ------------------------QLYGAPRIYKKVAPSSG--QIFDDPLFNFFKEVPDD 240
Query: 241 QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSCSA 300
QWVR+ DFTPS+ +GQSSSLCLKL N R+LPNF ++FAYY+E EDEF+ VDGGN FSCS
Sbjct: 241 QWVRTADFTPSNSVGQSSSLCLKLPNGRQLPNFRRHFAYYEEFEDEFKLVDGGNSFSCSD 300
Query: 301 DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSRIKIEFVEHALDR 360
DLVP+VDSRP++ LPY+IIFK+N LVQ+GCI+GP+LDT FY LVDPSRI++E+VE+AL++
Sbjct: 301 DLVPVVDSRPNVLLPYEIIFKVNVLVQNGCIAGPSLDTGFYRLVDPSRIRVEYVENALEK 360
Query: 361 LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420
LFHLKEC Y+P S+LTEQYKKY + P NSPAI+LD GLVYVRRVQITPCKVYFCGPEVNV
Sbjct: 361 LFHLKECCYEPSSFLTEQYKKYAKHPSNSPAIALDDGLVYVRRVQITPCKVYFCGPEVNV 420
Query: 421 SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI 480
SNRVLRHFSRD+DNFLRVSFVDEEWDKMRS DLLPR +S+I++ KT+IY+RILSVLKNGI
Sbjct: 421 SNRVLRHFSRDLDNFLRVSFVDEEWDKMRSTDLLPRMASSIENGKTDIYRRILSVLKNGI 480
Query: 481 VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPAKYAARLGQSF 540
IG K FKFLAFSSSQLR+NSLWMFA+RPGLDAA IR WMGDFR IKNPAKYAARLGQSF
Sbjct: 481 AIGDKKFKFLAFSSSQLRENSLWMFASRPGLDAAVIREWMGDFRHIKNPAKYAARLGQSF 540
Query: 541 GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF 600
GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAK+V++KC L AFTPSAF
Sbjct: 541 GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKRVASKCVL-AFTPSAF 600
Query: 601 QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660
QIRYGGYKGVVAVDP SS KLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST
Sbjct: 601 QIRYGGYKGVVAVDPRSSVKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660
Query: 661 LGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGYKPNVEPY 720
LGV+DEVFEKKQ EA++QLDAILTDPLKAQEAL+LM+PGENTN+LKEMLKCGYKP+VEPY
Sbjct: 661 LGVKDEVFEKKQSEALEQLDAILTDPLKAQEALELMSPGENTNILKEMLKCGYKPDVEPY 720
Query: 721 LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTKQSILSVT 780
LSMMLQ FR SKLL+LRTKSR+FIPNGRAMMGCLDETG+LEYGQVFVQ+S + +S
Sbjct: 721 LSMMLQTFRESKLLELRTKSRIFIPNGRAMMGCLDETGTLEYGQVFVQISSVRHRHIS-D 780
Query: 781 TSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDCVVFPQKGSRPH 840
TSAF+M+ SE + I GNV VAKNPCLHPGD+RVL AVNVP LYHMVDCVVFPQKGSRPH
Sbjct: 781 TSAFDMSGSEDHLVIVGNVTVAKNPCLHPGDVRVLNAVNVPLLYHMVDCVVFPQKGSRPH 840
Query: 841 PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN 900
PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPP QLDRDVTIEDVQEYFVNYMVN
Sbjct: 841 PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPIQLDRDVTIEDVQEYFVNYMVN 900
Query: 901 DSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF 960
DSLGIIANAHTAFADKE FKARS+PCIELAKLFSIAVDFPKTGVPAIIP L VKEFPDF
Sbjct: 901 DSLGIIANAHTAFADKEPFKARSAPCIELAKLFSIAVDFPKTGVPAIIPSHLNVKEFPDF 960
Query: 961 MDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA 1020
M+KPD+PSYESKNVIGKLFRAVK IAP S+I FTR+VA +CYDSDME EG+EDYV+DA
Sbjct: 961 MEKPDRPSYESKNVIGKLFRAVKGIAPNISYIRSFTRDVAMRCYDSDMETEGFEDYVEDA 1020
Query: 1021 IYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART 1080
IY+K YDYKLGNLLDYYGIKSE+EI SGNIM MSKSFT++RDAEAINLA RSLRKEART
Sbjct: 1021 IYHKTIYDYKLGNLLDYYGIKSESEIFSGNIMRMSKSFTRRRDAEAINLAARSLRKEART 1080
Query: 1081 WFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL 1140
WFN +ESGPDS G DDLFAKASAWYHVTYH SYWGSYN+ M RDHYLSFPWCVY KL
Sbjct: 1081 WFNTKESGPDS----GSDDLFAKASAWYHVTYHHSYWGSYNDGMKRDHYLSFPWCVYHKL 1128
Query: 1141 MQIKENNVRRKQRASRLAALDSRFRNALNL 1171
MQ+KENN+RR+++ASRLA LD RF LNL
Sbjct: 1141 MQVKENNLRRREKASRLATLD-RFSRVLNL 1128
BLAST of MS020748 vs. ExPASy TrEMBL
Match:
A0A6J1FSA2 (RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111447985 PE=3 SV=1)
HSP 1 Score: 1892.5 bits (4901), Expect = 0.0e+00
Identity = 935/1170 (79.91%), Postives = 1031/1170 (88.12%), Query Frame = 0
Query: 1 MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60
MGKTVHISGFP+ TAD+V+S+LE RTG+GTVYA+K+R PK+GGSR+YA VQF G+ +AE
Sbjct: 15 MGKTVHISGFPSHATADSVKSFLELRTGLGTVYAIKVRPPKRGGSRVYAIVQFIGAAQAE 74
Query: 61 LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120
LIISLAN QRLWYG+SYLKAR ADVDI+PKPRTY+HTLEDLTLCFGCQVSSEKF VLW
Sbjct: 75 LIISLAN---QRLWYGSSYLKARAADVDIVPKPRTYVHTLEDLTLCFGCQVSSEKFHVLW 134
Query: 121 EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLGSN 180
EG LVTFG MRKMNF L HNFVEYRF+LSYENIW+I+LH +HQSMK L+I
Sbjct: 135 EGDVELVTFGVEMRKMNFHLIHNFVEYRFQLSYENIWQIQLHRLQHQSMKCLLI------ 194
Query: 181 SFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVPDD 240
QLYGAPRIYKKV SSG ++FDDPL N+FKEVPDD
Sbjct: 195 ------------------------QLYGAPRIYKKVAPSSG--QIFDDPLFNFFKEVPDD 254
Query: 241 QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSCSA 300
QWVR+ DFTPS+ +GQSSSLCLKL N R+LPNF ++FAYY+E EDEF+ VDGGN FSCS
Sbjct: 255 QWVRTADFTPSNSVGQSSSLCLKLPNGRQLPNFRRHFAYYEEFEDEFKLVDGGNSFSCSD 314
Query: 301 DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSRIKIEFVEHALDR 360
DLVP+VDSRP++ LPY+IIFK+N LVQ+GCI+GP+LDT FY LVDPSRI++E+VE+AL++
Sbjct: 315 DLVPVVDSRPNVLLPYEIIFKVNVLVQNGCIAGPSLDTGFYRLVDPSRIRVEYVENALEK 374
Query: 361 LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420
LFHLKEC Y+P S+LTEQYKKY + P NSPAI+LD GLVYVRRVQITPCKVYFCGPEVNV
Sbjct: 375 LFHLKECCYEPSSFLTEQYKKYAKHPSNSPAIALDDGLVYVRRVQITPCKVYFCGPEVNV 434
Query: 421 SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI 480
SNRVLRHFSRD+DNFLRVSFVDEEWDKMRS DLLPR +S+I++ KT+IY+RILSVLKNGI
Sbjct: 435 SNRVLRHFSRDLDNFLRVSFVDEEWDKMRSTDLLPRMASSIENGKTDIYRRILSVLKNGI 494
Query: 481 VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPAKYAARLGQSF 540
IG K FKFLAFSSSQLR+NSLWMFA+RPGLDAA IR WMGDFR IKNPAKYAARLGQSF
Sbjct: 495 AIGDKKFKFLAFSSSQLRENSLWMFASRPGLDAAVIREWMGDFRHIKNPAKYAARLGQSF 554
Query: 541 GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF 600
GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAK+V++KC L AFTPSAF
Sbjct: 555 GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKRVASKCVL-AFTPSAF 614
Query: 601 QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660
QIRYGGYKGVVAVDP SS KLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST
Sbjct: 615 QIRYGGYKGVVAVDPRSSVKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 674
Query: 661 LGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGYKPNVEPY 720
LGV+DEVFEKKQ EA++QLDAILTDPLKAQEAL+LM+PGENTN+LKEMLKCGYKP+VEPY
Sbjct: 675 LGVKDEVFEKKQSEALEQLDAILTDPLKAQEALELMSPGENTNILKEMLKCGYKPDVEPY 734
Query: 721 LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTKQSILSVT 780
LSMMLQ FR SKLL+LRTKSR+FIPNGRAMMGCLDETG+LEYGQVFVQ+S + +S
Sbjct: 735 LSMMLQTFRESKLLELRTKSRIFIPNGRAMMGCLDETGTLEYGQVFVQISSVRHRHIS-D 794
Query: 781 TSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDCVVFPQKGSRPH 840
TSAF+M+ SE + I GNV VAKNPCLHPGD+RVL AVNVP LYHMVDCVVFPQKGSRPH
Sbjct: 795 TSAFDMSGSEDHLVIVGNVTVAKNPCLHPGDVRVLNAVNVPLLYHMVDCVVFPQKGSRPH 854
Query: 841 PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN 900
PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPP QLDRDVTIEDVQEYFVNYMVN
Sbjct: 855 PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPIQLDRDVTIEDVQEYFVNYMVN 914
Query: 901 DSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF 960
DSLGIIANAHTAFADKE FKARS+PCIELAKLFSIAVDFPKTGVPAIIP L VKEFPDF
Sbjct: 915 DSLGIIANAHTAFADKEPFKARSAPCIELAKLFSIAVDFPKTGVPAIIPSHLNVKEFPDF 974
Query: 961 MDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA 1020
M+KPD+PSYESKNVIGKLFRAVK IAP S+I FTR+VA +CYDSDME EG+EDYV+DA
Sbjct: 975 MEKPDRPSYESKNVIGKLFRAVKGIAPNISYIRSFTRDVAMRCYDSDMETEGFEDYVEDA 1034
Query: 1021 IYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART 1080
IY+K YDYKLGNLLDYYGIKSE+EI SGNIM MSKSFT++RDAEAINLA RSLRKEART
Sbjct: 1035 IYHKTIYDYKLGNLLDYYGIKSESEIFSGNIMRMSKSFTRRRDAEAINLAARSLRKEART 1094
Query: 1081 WFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL 1140
WFN +ESGPDS G DDLFAKASAWYHVTYH SYWGSYN+ M RDHYLSFPWCVY KL
Sbjct: 1095 WFNTKESGPDS----GSDDLFAKASAWYHVTYHHSYWGSYNDGMKRDHYLSFPWCVYHKL 1142
Query: 1141 MQIKENNVRRKQRASRLAALDSRFRNALNL 1171
MQ+KENN+RR+++ASRLA LD RF LNL
Sbjct: 1155 MQVKENNLRRREKASRLATLD-RFSRVLNL 1142
BLAST of MS020748 vs. ExPASy TrEMBL
Match:
A0A6J1K2P9 (RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111490498 PE=3 SV=1)
HSP 1 Score: 1880.1 bits (4869), Expect = 0.0e+00
Identity = 933/1170 (79.74%), Postives = 1028/1170 (87.86%), Query Frame = 0
Query: 1 MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60
MGKTVHISGFP+ TAD+V+S+LE RTG+GTVYA+K+R PK+GGSR+YA VQFTG+ +AE
Sbjct: 1 MGKTVHISGFPSHATADSVKSFLELRTGLGTVYAIKVRPPKRGGSRVYAIVQFTGAAQAE 60
Query: 61 LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120
LIISLAN QRLWYG+SYLKAR ADVDI+PKPRTY+HTLE+LTLCFGCQVSSEKF VLW
Sbjct: 61 LIISLAN---QRLWYGSSYLKARAADVDIVPKPRTYVHTLENLTLCFGCQVSSEKFHVLW 120
Query: 121 EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLGSN 180
EG LVTFG MRKMNF L HNFVEYRF+LSYENIW+I+LH +HQSMK L+I
Sbjct: 121 EGDVELVTFGVEMRKMNFHLIHNFVEYRFQLSYENIWQIQLHRLQHQSMKCLLI------ 180
Query: 181 SFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVPDD 240
QLYGAPRIYKKV SSG ++FDDPL N+FKEVPDD
Sbjct: 181 ------------------------QLYGAPRIYKKVAPSSG--QIFDDPLFNFFKEVPDD 240
Query: 241 QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSCSA 300
Q VR+ DFTPS IGQSSSLCLKL N R+LPNF ++FAYY+E EDEF+ VDGGNGFSCS
Sbjct: 241 QCVRTADFTPSYSIGQSSSLCLKLPNGRQLPNFRRHFAYYEEFEDEFKLVDGGNGFSCSD 300
Query: 301 DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSRIKIEFVEHALDR 360
DLVP+VDS P++ LPY+IIFK+N LVQ+GCI GP+LDT FY LVDPSRI++E+VE+AL++
Sbjct: 301 DLVPVVDSHPNVLLPYEIIFKVNVLVQNGCIPGPSLDTGFYRLVDPSRIRVEYVENALEK 360
Query: 361 LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420
LFHLKEC Y+P S+LTEQYKKY + P NSPAI+LDAGLVYVRRVQITPCKVYFCGPEVNV
Sbjct: 361 LFHLKECCYEPSSFLTEQYKKYAKHPSNSPAIALDAGLVYVRRVQITPCKVYFCGPEVNV 420
Query: 421 SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI 480
SNRVLRHFSRD+DNFLRVSFVDEEWDKMRS DLLPR +S+I++ KT+IY+RILSVLKNGI
Sbjct: 421 SNRVLRHFSRDLDNFLRVSFVDEEWDKMRSTDLLPRMASSIENGKTDIYRRILSVLKNGI 480
Query: 481 VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPAKYAARLGQSF 540
IG K FKFLAFSSSQLR+NSLWMFA+RPGLDAA IR WMGDF+ IKNPAKYAARLGQSF
Sbjct: 481 AIGDKKFKFLAFSSSQLRENSLWMFASRPGLDAAVIREWMGDFQHIKNPAKYAARLGQSF 540
Query: 541 GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF 600
GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAK+V++KC L AFTPSAF
Sbjct: 541 GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKRVASKCVL-AFTPSAF 600
Query: 601 QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660
QIRYGGYKGVVAVDP SS KLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST
Sbjct: 601 QIRYGGYKGVVAVDPRSSVKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660
Query: 661 LGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGYKPNVEPY 720
LGV+DEVFEKKQ EA++QLDAILTDPLKAQEAL+LM+PGENTN+LKEMLKCGYKP+VEPY
Sbjct: 661 LGVKDEVFEKKQSEALEQLDAILTDPLKAQEALELMSPGENTNILKEMLKCGYKPDVEPY 720
Query: 721 LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTKQSILSVT 780
LSMMLQ FR SKLL+LRTKSR+FIPNGRAMMGCLDETG+LEYGQVFVQ+S + +S
Sbjct: 721 LSMMLQTFRESKLLELRTKSRIFIPNGRAMMGCLDETGTLEYGQVFVQISNARHRHIS-D 780
Query: 781 TSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDCVVFPQKGSRPH 840
TSAF+M+ SE + I GNV VAKNPCLHPGD+RVL AVNVP LYHMVDCVVFPQKGSRPH
Sbjct: 781 TSAFDMSGSEDHLVIVGNVTVAKNPCLHPGDVRVLNAVNVPLLYHMVDCVVFPQKGSRPH 840
Query: 841 PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN 900
PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPP QLDRDVTIEDVQEYFVNYMVN
Sbjct: 841 PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPIQLDRDVTIEDVQEYFVNYMVN 900
Query: 901 DSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF 960
DSLGIIANAHTAFADKE FKARS+PCIELAKLFSIAVDFPKTGVPAIIP L VKEFPDF
Sbjct: 901 DSLGIIANAHTAFADKEPFKARSAPCIELAKLFSIAVDFPKTGVPAIIPSHLNVKEFPDF 960
Query: 961 MDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA 1020
M+KPD+ SYESKNVIGKLFRAVK IAP S+I FTR+VA +CYDSDME EG+EDYV+DA
Sbjct: 961 MEKPDRHSYESKNVIGKLFRAVKGIAPNISYIRSFTRDVAMRCYDSDMEAEGFEDYVEDA 1020
Query: 1021 IYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART 1080
IY+K YDYKLGNLLDYYGIKSE+EI SGNIM MSKSFT++RDAEAINLA RSLRKEART
Sbjct: 1021 IYHKTIYDYKLGNLLDYYGIKSESEIFSGNIMRMSKSFTRRRDAEAINLAARSLRKEART 1080
Query: 1081 WFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL 1140
WFN +ESG DS G DDLFAKASAWYHVTYH SYWGSYN+ M RDHYLSFPWCVY KL
Sbjct: 1081 WFNTKESGLDS----GSDDLFAKASAWYHVTYHHSYWGSYNDGMKRDHYLSFPWCVYHKL 1128
Query: 1141 MQIKENNVRRKQRASRLAALDSRFRNALNL 1171
MQ+KENN+R+++RASRLA LD RF LNL
Sbjct: 1141 MQVKENNLRKRERASRLATLD-RFSRVLNL 1128
BLAST of MS020748 vs. TAIR 10
Match:
AT1G14790.1 (RNA-dependent RNA polymerase 1 )
HSP 1 Score: 1407.5 bits (3642), Expect = 0.0e+00
Identity = 711/1168 (60.87%), Postives = 871/1168 (74.57%), Query Frame = 0
Query: 1 MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60
MGKT+ + GFP GV+A+ V+ +LE TG GTVYA+K+R+PKKGG R+YA VQFT R
Sbjct: 1 MGKTIQVFGFPNGVSAEEVKKFLERLTGSGTVYAIKVRQPKKGGPRVYAIVQFTSERHTR 60
Query: 61 LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120
LII+ A +RL+YG SYLKA E + DI+PKPR +HT+ L + FGCQVS++KF LW
Sbjct: 61 LIITAA---AERLYYGRSYLKAFEVEQDIVPKPRASLHTISGLKMFFGCQVSTKKFLTLW 120
Query: 121 EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLGSN 180
+DV V+FG GMRK++F S +YR ELSYENIW+I+LH + +S K+LVIQV+
Sbjct: 121 SAQDVCVSFGIGMRKLHFSFSWYQKDYRLELSYENIWQIDLHSPQGRSSKFLVIQVI--- 180
Query: 181 SFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVPDD 240
GAP+I++K + +F +++++ + D+
Sbjct: 181 ---------------------------GAPKIFEKEDQP--INLLFG--IMDFYSDGSDE 240
Query: 241 QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSCSA 300
QW+R+TDFT SSCIGQS++ CL+L +P+F +NFA Y E ++ G+ +S +A
Sbjct: 241 QWIRTTDFTSSSCIGQSTAFCLELPVHLNVPDFRENFANYAEHRASSFLIESGSSYSSNA 300
Query: 301 D-LVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSRIKIEFVEHALD 360
+ LVP+VD P LP++I+FK+N LVQ+ C+SGPALD FY L++ + ++H L+
Sbjct: 301 NTLVPVVDPPPGFSLPFEILFKLNTLVQNACLSGPALDLDFYRLLNQKKYDRALIDHCLE 360
Query: 361 RLFHLKECEYKPYSWLTEQYKKYLRRP--PNSPAISLDAGLVYVRRVQITPCKVYFCGPE 420
+LFHL EC Y+P WL ++YKK++ + P SP ISLD GLVY+ RVQ+TP +VYF GPE
Sbjct: 361 KLFHLGECCYEPAHWLRDEYKKWISKGKLPLSPTISLDDGLVYMYRVQVTPARVYFSGPE 420
Query: 421 VNVSNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLK 480
VNVSNRVLRH+S+ I+NFLRVSFVDE+ +K+RS+DL PR+S+ R+T++Y RI SVL+
Sbjct: 421 VNVSNRVLRHYSKYINNFLRVSFVDEDLEKVRSMDLSPRSST---QRRTKLYDRIYSVLR 480
Query: 481 NGIVIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPAKYAARLG 540
+GIVIG K F+FLAFSSSQLR+NS WMFA + AA IRAWMGDF I+N AKYAARLG
Sbjct: 481 DGIVIGDKKFEFLAFSSSQLRENSAWMFAPIDRITAAHIRAWMGDFDHIRNVAKYAARLG 540
Query: 541 QSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTP 600
QSF SS ETL+V E E+IPD+E+ +YVFSDGIGKIS++FA+KV+ KCGL F+P
Sbjct: 541 QSFSSSRETLNVRSDEIEVIPDVEIISLGTRYVFSDGIGKISAEFARKVARKCGLTEFSP 600
Query: 601 SAFQIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITL 660
SAFQIRYGGYKGVVAVDP SSKKLSLRKSM KFES+NTKLDVL +SKYQPC++NRQLITL
Sbjct: 601 SAFQIRYGGYKGVVAVDPNSSKKLSLRKSMSKFESENTKLDVLAWSKYQPCYMNRQLITL 660
Query: 661 LSTLGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGYKPNV 720
LSTLGV D VFEKKQ E V +LDAILT PL+A EAL LMAPGENTN+LK ++ CGYKP+
Sbjct: 661 LSTLGVTDSVFEKKQREVVDRLDAILTHPLEAHEALGLMAPGENTNILKALILCGYKPDA 720
Query: 721 EPYLSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTKQSIL 780
EP+LSMMLQ FRASKLL+LRTK+R+FI GR+MMGCLDET +LEYGQV VQ S +
Sbjct: 721 EPFLSMMLQNFRASKLLELRTKTRIFISGGRSMMGCLDETRTLEYGQVVVQYSDPMR--- 780
Query: 781 SVTTSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDCVVFPQKGS 840
+R I G VVVAKNPCLHPGD+RVL+AVNVP L HMVDCVVFPQKG
Sbjct: 781 -----------PGRRFIITGPVVVAKNPCLHPGDVRVLQAVNVPALNHMVDCVVFPQKGL 840
Query: 841 RPHPNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNY 900
RPHPNECSGSDLDGDIYFVCWD EL+PPR PMDYTP P LD DVTIE+V+EYF NY
Sbjct: 841 RPHPNECSGSDLDGDIYFVCWDQELVPPRTSEPMDYTPEPTQILDHDVTIEEVEEYFANY 900
Query: 901 MVNDSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIPPELYVKEF 960
+VNDSLGIIANAHTAFADKE KA S PCIELAK FS AVDFPKTGV A+IP LYVKE+
Sbjct: 901 IVNDSLGIIANAHTAFADKEPLKAFSDPCIELAKKFSTAVDFPKTGVAAVIPQHLYVKEY 960
Query: 961 PDFMDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYV 1020
PDFM+KPDKP+YESKNVIGKLFR VK+ AP I FT +VA + YD DME++G+E+YV
Sbjct: 961 PDFMEKPDKPTYESKNVIGKLFREVKERAPPLISIKSFTLDVASKSYDKDMEVDGFEEYV 1020
Query: 1021 DDAIYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKE 1080
D+A Y K NYD+KLGNL+DYYGIK+EAEILSG IM MSKSFTK+RDAE+I A+R+LRKE
Sbjct: 1021 DEAFYQKANYDFKLGNLMDYYGIKTEAEILSGGIMRMSKSFTKRRDAESIGRAVRALRKE 1080
Query: 1081 ARTWFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVY 1140
+ FN E + AKASAWYHVTYH SYWG YNE +NRDH+LSF WCVY
Sbjct: 1081 TLSLFNASEEEENES---------AKASAWYHVTYHSSYWGLYNEGLNRDHFLSFAWCVY 1105
Query: 1141 DKLMQIKENNVRRKQRASRLAALDSRFR 1166
DKL++IK+ N+ R+QR L LD R
Sbjct: 1141 DKLVRIKKTNLGRRQRQETLERLDHVLR 1105
BLAST of MS020748 vs. TAIR 10
Match:
AT4G11130.1 (RNA-dependent RNA polymerase 2 )
HSP 1 Score: 758.4 bits (1957), Expect = 8.2e-219
Identity = 466/1181 (39.46%), Postives = 678/1181 (57.41%), Query Frame = 0
Query: 4 TVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGS-RLYAFVQFTGSREAELI 63
TV IS P + AD + +LE G TV+AL+I + R +A VQFT
Sbjct: 11 TVKISNVPQTIVADELLRFLELHLGEDTVFALEIPTTRDNWKPRDFARVQFTTLEVKSRA 70
Query: 64 ISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEK-FCVL-- 123
L++Q +L + T L+ EA DIIP+P L+D+ L G S EK FC L
Sbjct: 71 QLLSSQ--SKLLFKTHNLRLSEAYDDIIPRPVDPRKRLDDIVLTVGFPESDEKRFCALEK 130
Query: 124 WEG-RDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLG 183
W+G R ++T R++ F + + Y+ E+ +E+I ++ L V G
Sbjct: 131 WDGVRCWILT---EKRRVEFWVWESGDCYKIEVRFEDI------------IETLSCCVNG 190
Query: 184 SNSFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVP 243
S ++ +L LK YG P+++K+V ++ F + KE
Sbjct: 191 DAS--EIDAFLLKLK-------------YG-PKVFKRVTVHIATK--FKSDRYRFCKEDF 250
Query: 244 DDQWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSC 303
D W+R+TDF+ S IG S+ CL++HN + + YY+E +VD G F+
Sbjct: 251 DFMWIRTTDFSGSKSIGTSTCFCLEVHNGSTMLDIFSGLPYYREDTLSLTYVD-GKTFAS 310
Query: 304 SADLVPMVDSR-PDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSR-IKIEFVEH 363
+A +VP++++ + PY+I+F++NALV IS S L+ R + +E
Sbjct: 311 AAQIVPLLNAAILGLEFPYEILFQLNALVHAQKIS--LFAASDMELIKILRGMSLETALV 370
Query: 364 ALDRLFHLKECEYKPYSWLTEQYKKYLRRPPNSPAIS----LDAGLVYVRRVQITPCKVY 423
L +L Y P ++ Q + +++ +SPA + + ++ +R +TP K+Y
Sbjct: 371 ILKKLHQQSSICYDPVFFVKTQMQSVVKKMKHSPASAYKRLTEQNIMSCQRAYVTPSKIY 430
Query: 424 FCGPEVNVSNRVLRHFSRDIDNFLRVSFVDEEWDKM--RSIDLLPRTSSTIKDRKTEIYK 483
GPE+ +N V+++F+ + +F+RV+FV+E+W K+ ++ + + +K +T IY
Sbjct: 431 LLGPELETANYVVKNFAEHVSDFMRVTFVEEDWSKLPANALSVNSKEGYFVKPSRTNIYN 490
Query: 484 RILSVLKNGIVIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPA 543
R+LS+L GI +G K F+FLAFS+SQLR NS+WMFA+ + A DIR WMG FR+I++ +
Sbjct: 491 RVLSILGEGITVGPKRFEFLAFSASQLRGNSVWMFASNEKVKAEDIREWMGCFRKIRSIS 550
Query: 544 KYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKC 603
K AAR+GQ F +S +TL V + E IPDIEV Y FSDGIGKIS FAK+V+ KC
Sbjct: 551 KCAARMGQLFSASRQTLIVRAQDVEQIPDIEVTTDGADYCFSDGIGKISLAFAKQVAQKC 610
Query: 604 GLKAFTPSAFQIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFL 663
GL + PSAFQIRYGGYKGV+AVD S +KLSLR SM KF+S+N L+V +++ PCFL
Sbjct: 611 GL-SHVPSAFQIRYGGYKGVIAVDRSSFRKLSLRDSMLKFDSNNRMLNVTRWTESMPCFL 670
Query: 664 NRQLITLLSTLGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLK 723
NR++I LLSTLG+ D +FE Q + L +L D A L ++ + N+L +ML
Sbjct: 671 NREIICLLSTLGIEDAMFEAMQAVHLSMLGNMLEDRDAALNVLQKLSGENSKNLLVKMLL 730
Query: 724 CGYKPNVEPYLSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVS 783
GY P+ EPYLSMML+ S+L +L+++ R+ +P GR ++GC+DE G LEYGQV+V+V+
Sbjct: 731 QGYAPSSEPYLSMMLRVHHESQLSELKSRCRILVPKGRILIGCMDEMGILEYGQVYVRVT 790
Query: 784 GTKQSILSVTTSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYH--MVD 843
TK + S S F E V I G VVV KNPCLHPGDIRVL A+ +D
Sbjct: 791 LTKAELKSRDQSYFRKIDEETSVVI-GKVVVTKNPCLHPGDIRVLDAIYEVHFEEKGYLD 850
Query: 844 CVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIE 903
C++FPQKG RPHPNECSG DLDGD +FV WD ++IP PPMDY + P +D DVT+E
Sbjct: 851 CIIFPQKGERPHPNECSGGDLDGDQFFVSWDEKIIPSEMDPPMDYAGSRPRLMDHDVTLE 910
Query: 904 DVQEYFVNYMVNDSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAII 963
++ ++FV+YM++D+LG+I+ AH AD++ KARS C+ELA L S AVDF KTG PA +
Sbjct: 911 EIHKFFVDYMISDTLGVISTAHLVHADRDPEKARSQKCLELANLHSRAVDFAKTGAPAEM 970
Query: 964 PPELYVKEFPDFMDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDM 1023
P L +EFPDF+++ +KP+Y S++V GKL+RAVK + VA YD +
Sbjct: 971 PYALKPREFPDFLERFEKPTYISESVFGKLYRAVKSSLAQRKPEAESEDTVA---YDVTL 1030
Query: 1024 EIEGYEDYVDDAIYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRD----- 1083
E G+E +++ A +++ Y KL +L+ YYG +E EIL+G + T K RD
Sbjct: 1031 EEAGFESFIETAKAHRDMYGEKLTSLMIYYGAANEEEILTGILKT--KEMYLARDNRRYG 1090
Query: 1084 --AEAINLAIRSLRKEARTWFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYN 1143
+ I L+++ L KEA WF + L ASAWY+VTY+P N
Sbjct: 1091 DMKDRITLSVKDLHKEAMGWFEKSCEDEQQKKKL--------ASAWYYVTYNP------N 1132
Query: 1144 EDMNRDHYLSFPWCVYDKLMQIKENNVRRKQRASRLAALDS 1163
+ +LSFPW V D L+ IK N +R+ + + L S
Sbjct: 1151 HRDEKLTFLSFPWIVGDVLLDIKAENAQRQSVEEKTSGLVS 1132
BLAST of MS020748 vs. TAIR 10
Match:
AT3G49500.1 (RNA-dependent RNA polymerase 6 )
HSP 1 Score: 589.0 bits (1517), Expect = 8.6e-168
Identity = 427/1204 (35.47%), Postives = 611/1204 (50.75%), Query Frame = 0
Query: 5 VHISGFPAGVTADAVRSYLEGRTGVGTVYALK----------------------IREPKK 64
V I GF TA + YLE G+ LK I E KK
Sbjct: 15 VSIGGFGESTTAKQLTDYLEDEVGIVWRCRLKTSWTPPGSYPNFEIADTSNIPSIDEYKK 74
Query: 65 GGSRLYAFVQFTGSREAELIISLANQRIQRLWYGTSYLKAREADVDIIPK---------P 124
+AFV F A + A Q L + V + PK
Sbjct: 75 --VEPHAFVHFAVFESAGRAMDAAGQ-------CNLILDGQPLKVSLGPKNPYSLNQRRR 134
Query: 125 RTYMHTLEDLTLCFGCQVSSEKFCVLW--EGRDVLVTFGFGMRKMNFRLSHNFVEYRFEL 184
T + L +TL G VS + F V W EG D LV K FR S F ++ +
Sbjct: 135 TTVPYKLAGITLEIGTLVSRDDFFVSWRAEGVDFLVDPFDNTCKFCFRKSTAF-SFKDAV 194
Query: 185 SYENIWRIELHHSRHQSMKYLVIQVLGSNSFPKLSHAILMLKIHQIIVMFFFSQLYGAPR 244
+ I + ++ LV + + L +L+L QL +PR
Sbjct: 195 MHAVI-------NCDYKLELLVRDIQTVRQYKTLHGFVLIL------------QLASSPR 254
Query: 245 IYKKVESSSGSRRVFDDPLLNYFKEVPDDQWVRSTDFTPSSCIGQSSS--LCLKLHNCRE 304
++ + V D L + DD W+R+TDFT IG+ S + + +
Sbjct: 255 VWYRTADDDIYDTVPGDLLDD------DDPWIRTTDFTQVGAIGRCHSYRVLISPRYENK 314
Query: 305 LPNFGQNFAYYKETEDEFRFVDGGNGFSCSADLVPMVDSRPDIF----LPYKIIFKINAL 364
L F + E+ R+ C + P+ D I + ++I+F +N++
Sbjct: 315 LRTALDYFRMRRVQEERVRWPPRIRNEPCFGE--PVSDHFFCIHHKEGISFEIMFLVNSV 374
Query: 365 VQHGCISGPALDTSFYHLV--DPSRIKIEFVEHALDRLFHLKECEYKPYSWLTEQYKKYL 424
+ G + L F+ L+ P + I ++H L K + Y L +
Sbjct: 375 LHRGVFNQFQLTERFFDLLRNQPKDVNIASLKH----LCTYKRPVFDAYKRLKLVQEWIQ 434
Query: 425 RRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNVSNRVLRHFSRDIDNFLRVSFVDE 484
+ P + + +RR+ ITP + Y PEV +SNRVLR + + FLRV+F+DE
Sbjct: 435 KNPKLLGSHEQSEDISEIRRLVITPTRAYCLPPEVELSNRVLRRYKAVAERFLRVTFMDE 494
Query: 485 EWDKMRSIDLLPRTSSTIKD-------RKTEIYKRILSVLKNGIVIGGKMFKFLAFSSSQ 544
+ S L + +KD +KT ++KR+ S+L +G + G+ + FLAFS++Q
Sbjct: 495 SMQTINSNVLSYFVAPIVKDLTSSSFSQKTYVFKRVKSILTDGFKLCGRKYSFLAFSANQ 554
Query: 545 LRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPAKYAARLGQSFGSSTETLSVARHEREI 604
LRD S W FA +DI+ WMG F+ KN AK AAR+G F S+ T+ V HE +
Sbjct: 555 LRDRSAWFFAEDGKTRVSDIKTWMGKFKD-KNVAKCAARMGLCFSSTYATVDVMPHEVDT 614
Query: 605 -IPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKA-FTPSAFQIRYGGYKGVVAVD 664
+PDIE ++G YVFSDGIG I+ D A +V K L ++P A+QIRY G+KGVVA
Sbjct: 615 EVPDIE-RNG---YVFSDGIGTITPDLADEVMEKLKLDVHYSPCAYQIRYAGFKGVVARW 674
Query: 665 PYSSK--KLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLSTLGVRDEVFEKKQ 724
P S +L+LR SM KF S +T L++ ++++QP FLNRQ+ITLLS LGV DE+F Q
Sbjct: 675 PSKSDGIRLALRDSMKKFFSKHTILEICSWTRFQPGFLNRQIITLLSVLGVPDEIFWDMQ 734
Query: 725 HEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGYKPNVEPYLSMMLQAFRASK 784
+ +L+ IL D A E L E N ML G+KP EP+L ML + R ++
Sbjct: 735 ESMLYKLNRILDDTDVAFEVLTASC-AEQGNTAAIMLSAGFKPKTEPHLRGMLSSVRIAQ 794
Query: 785 LLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTK-QSILSVTTSAFNMNSSEQ 844
L LR KSR+F+ +GR +MGCLDE G LE+GQ F+QVS ++ S S F ++
Sbjct: 795 LWGLREKSRIFVTSGRWLMGCLDEAGILEHGQCFIQVSKPSIENCFSKHGSRFKETKTDL 854
Query: 845 RVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDCVVFPQKGSRPHPNECSGSDLDG 904
V +KG V +AKNPCLHPGD+R+L+AV+VPQL+HM DC++FPQKG RPH NE SGSDLDG
Sbjct: 855 EV-VKGYVAIAKNPCLHPGDVRILEAVDVPQLHHMYDCLIFPQKGDRPHTNEASGSDLDG 914
Query: 905 DIYFVCWDTELIPP--RQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVNDSLGIIANA 964
D+YFV WD +LIPP + P M Y A L R V +D+ ++F + N+ LG I NA
Sbjct: 915 DLYFVAWDQKLIPPNRKSYPAMHYDAAEEKSLGRAVNHQDIIDFFARNLANEQLGTICNA 974
Query: 965 HTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIPPELYVKEFPDFMDKPDKPSY 1024
H AD+ + A C+ LA+L + AVDFPKTG +P L K +PDFM K D +Y
Sbjct: 975 HVVHADRSEYGAMDEECLLLAELAATAVDFPKTGKIVSMPFHLKPKLYPDFMGKEDYQTY 1034
Query: 1025 ESKNVIGKLFRAVKDIAPTTSHISL-FTREVARQCYDSDMEIEGYEDYVDDAIYYKNNYD 1084
+S ++G+L+R VK++ + S + + + YD+ +EI G+ED + +A +K YD
Sbjct: 1035 KSNKILGRLYRRVKEVYDEDAEASSEESTDPSAIPYDAVLEIPGFEDLIPEAWGHKCLYD 1094
Query: 1085 YKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRD--AEAINLAIRSLRKEARTWFNERE 1144
+L LL Y ++ E EI++G+I +M K +KK+ E + + SL+KE R F E
Sbjct: 1095 GQLIGLLGQYKVQKEEEIVTGHIWSMPKYTSKKQGELKERLKHSYNSLKKEFRKVFEETI 1154
BLAST of MS020748 vs. TAIR 10
Match:
AT2G19910.1 (RNA-dependent RNA polymerase family protein )
HSP 1 Score: 114.4 bits (285), Expect = 6.2e-25
Identity = 120/474 (25.32%), Postives = 195/474 (41.14%), Query Frame = 0
Query: 570 VFSDGIGKISSDFAK---------------KVSAK---------CGLKAFTPSAFQIRYG 629
+ SDG G IS D A+ + +K CG + F+I Y
Sbjct: 401 IHSDGTGYISEDLARMCPVNIFKGKSMRSNNIQSKNLNFEGQGPCGQEPPLLIQFRIFYN 460
Query: 630 GY--KGVVAVD-PYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQ---------PCFLNRQ 689
GY KG + + + +R SM K D T ++ ++ + L+R
Sbjct: 461 GYAVKGTFLTNKKLPPRTVQVRPSMIKVYEDRTLSNLSTFNSLEVVTTSNPPRKARLSRN 520
Query: 690 LITLLSTLGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGY 749
L+ LLS GV ++ F +++ I A +A + T +M+ G
Sbjct: 521 LVALLSYGGVPNDFFLNILRNTLEESKTIFYSERAAFKAAINYGDDQYT---ADMILVGI 580
Query: 750 KPNVEPYLSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTK 809
P EPYL L ++ L+ R I +MG +D TG L+ ++ V + +
Sbjct: 581 -PLDEPYLKDRLSYLLKTERNALKA-GRFPIDESYYIMGTVDPTGELKENEICVILHSGQ 640
Query: 810 QSILSVTTSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVD----C 869
I G+V+V +NP LH GDI VLKA V L V
Sbjct: 641 ---------------------ISGDVLVYRNPGLHFGDIHVLKATYVKALEDYVGNAKFA 700
Query: 870 VVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIP---------PRQIPPMDYTPAPPTQ 929
V FPQKG R +E +G D DGD+YF+ + +L+ P Y P++
Sbjct: 701 VFFPQKGPRSLGDEIAGGDFDGDMYFISRNPKLLEHFKPSEPWVSSSKPSKIYCGRKPSE 760
Query: 930 LDRDVTIEDVQEYFV--NYMVNDSLGIIANAHTAFAD----------KELFKARSSPCIE 983
L + E++ + F+ + D +G+ A+ D KE ++ R ++
Sbjct: 761 LSEEELEEELFKMFLKARFCKRDVIGMAADCWLGIMDPFLTLGDESAKEKYE-RKKNILK 820
BLAST of MS020748 vs. TAIR 10
Match:
AT2G19930.1 (RNA-dependent RNA polymerase family protein )
HSP 1 Score: 105.9 bits (263), Expect = 2.2e-22
Identity = 129/491 (26.27%), Postives = 209/491 (42.57%), Query Frame = 0
Query: 570 VFSDGIGKISSDFAKK-----VSAKCGLKAFTPSA----------FQIRYGGY--KG--- 629
+ SDG G IS D A+ KC A F++ Y GY KG
Sbjct: 397 IHSDGTGYISEDLARMCPLNIFKGKCLRSESIQEACYQDPPLLIQFRMFYDGYAVKGTFL 456
Query: 630 --------VVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLSTL 689
V V P S K+S S+ F + N V + + L++ L+ LLS
Sbjct: 457 LNKKLCPRTVQVRP-SMIKVSKDPSLSNFSTFNALEVVTTSNPPKRTKLSKNLVALLSYG 516
Query: 690 GVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGYKPNVEPYL 749
G+ +E F +++ +I + A A ++ N +M+ G P EP+L
Sbjct: 517 GIPNEFFLDILLNTLEESKSIFYNKRAALNAALNYGEMDDQNA-AQMILVGI-PLDEPHL 576
Query: 750 SMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTKQSILSVTT 809
L ++ DL+ ++ + +MG +D TG+L+ +V V + +
Sbjct: 577 KNYLSILLKTEKNDLKA-GKLPVTESYYLMGTVDPTGALKEDEVCVILESGQ-------- 636
Query: 810 SAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVD----CVVFPQKGS 869
I G V+V +NP LH GDI +LKA V L V V FPQKG
Sbjct: 637 -------------ISGEVLVYRNPGLHFGDIHILKATYVKALEEYVGNSKFAVFFPQKGP 696
Query: 870 RPHPNECSGSDLDGDIYFVCWDTELI---PPRQIPPMDYTP-------APPTQLDRDVTI 929
R +E +G D DGD+YF+ + EL+ P + P + TP P+QL +
Sbjct: 697 RSLGDEIAGGDFDGDMYFISRNPELLENFKPSE-PWVSLTPPSKSNSGRAPSQLSPEELE 756
Query: 930 EDVQEYFV--NYMVNDSLGIIANAHTAFADKELF--------KAR-SSPCIELAKLFSIA 989
E++ E F+ + ++ +GI A++ D+ L KA +EL ++ A
Sbjct: 757 EELFEMFLTAGFHASNVIGIAADSWLTIMDRFLILGDDRAEEKAEMKKKMLELIDIYYDA 816
Query: 990 VDFPKTGVPAIIPPELYVKEFPDFMDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFT 1008
+D PK G +P +L FP +M++ K ++S +++G +F VK + TT S +
Sbjct: 817 LDAPKKGDKVYLPNKLKPDIFPHYMERDKK--FQSTSILGLIFDFVK--SQTTEEPSPSS 857
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022146805.1 | 0.0e+00 | 95.90 | probable RNA-dependent RNA polymerase 1 isoform X1 [Momordica charantia] | [more] |
XP_022146806.1 | 0.0e+00 | 93.09 | probable RNA-dependent RNA polymerase 1 isoform X2 [Momordica charantia] | [more] |
XP_038891504.1 | 0.0e+00 | 79.76 | RNA-dependent RNA polymerase 1 isoform X1 [Benincasa hispida] >XP_038891505.1 RN... | [more] |
XP_022943173.1 | 0.0e+00 | 79.91 | RNA-dependent RNA polymerase 1-like isoform X2 [Cucurbita moschata] >XP_02294317... | [more] |
XP_022943172.1 | 0.0e+00 | 79.91 | RNA-dependent RNA polymerase 1-like isoform X1 [Cucurbita moschata] | [more] |
Match Name | E-value | Identity | Description | |
Q9LQV2 | 0.0e+00 | 60.87 | RNA-dependent RNA polymerase 1 OS=Arabidopsis thaliana OX=3702 GN=RDR1 PE=2 SV=1 | [more] |
Q0DXS3 | 1.5e-286 | 66.80 | Probable RNA-dependent RNA polymerase 1 OS=Oryza sativa subsp. japonica OX=39947... | [more] |
O82504 | 1.2e-217 | 39.46 | RNA-dependent RNA polymerase 2 OS=Arabidopsis thaliana OX=3702 GN=RDR2 PE=1 SV=1 | [more] |
Q7XM31 | 2.7e-206 | 37.00 | Probable RNA-dependent RNA polymerase 2 OS=Oryza sativa subsp. japonica OX=39947... | [more] |
Q8LHH9 | 4.9e-168 | 39.05 | Probable RNA-dependent RNA polymerase SHL2 OS=Oryza sativa subsp. japonica OX=39... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1CYE0 | 0.0e+00 | 95.90 | RNA-dependent RNA polymerase OS=Momordica charantia OX=3673 GN=LOC111015923 PE=3... | [more] |
A0A6J1D0H1 | 0.0e+00 | 93.09 | RNA-dependent RNA polymerase OS=Momordica charantia OX=3673 GN=LOC111015923 PE=3... | [more] |
A0A6J1FXH5 | 0.0e+00 | 79.91 | RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111447985 PE=3 ... | [more] |
A0A6J1FSA2 | 0.0e+00 | 79.91 | RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111447985 PE=3 ... | [more] |
A0A6J1K2P9 | 0.0e+00 | 79.74 | RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111490498 PE=3 SV... | [more] |