MS020748 (gene) Bitter gourd (TR) v1

Overview
NameMS020748
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionRNA-dependent RNA polymerase
Locationscaffold487: 109557 .. 114092 (-)
RNA-Seq ExpressionMS020748
SyntenyMS020748
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAAAAACAGTTCATATTAGTGGATTTCCTGCAGGTGTCACGGCAGATGCCGTTAGGAGTTATTTGGAGGGTCGTACGGGTGTTGGAACTGTCTATGCTCTTAAGATTAGAGAACCCAAAAAGGGGGGAAGTAGATTATATGCTTTTGTTCAATTCACTGGCTCTAGAGAAGCTGAGTTGATCATTTCATTGGCTAATCAACGGATTCAACGCCTGTGGTATGGAACTTCGTATCTCAAGGCTCGAGAAGCGGATGTTGATATTATACCGAAGCCTAGGACGTACATGCATACCTTGGAAGATTTGACATTGTGCTTTGGTTGTCAGGTCTCAAGTGAAAAGTTTTGTGTCCTGTGGGAAGGAAGGGATGTTTTGGTGACTTTTGGATTTGGAATGCGAAAAATGAACTTTCGTTTGTCTCATAACTTTGTAGAGTACAGGTTCGAGCTTTCGTACGAGAACATTTGGCGGATAGAACTCCATCATTCGCGGCATCAGTCCATGAAGTATCTTGTGATTCAGGTTCTGGGGTCAAATTCCTTTCCAAAGTTGTCTCATGCTATACTAATGTTGAAAAGTATTATTATCCTCTCTTAAAGTTGAAAAATGTTGCTACTTCTGTTTAAGCATTGTTTCAACTATTACATTAGTAAGTTGAACATCATAACGTTTTATCCTCGTTGAAAAAGTGTTGCTATCCCTCTTAATTGTTGGAATAAGAGTACTTTATTGAATGATATTTAGGTTGATGGGAACGACCAGGCAGCGCATCTAGATTTATTCACTTGCATATTATTTGCCATCTTCCAAGATATTACTGTATGACTCTAGATTTCCCCACTTGCATTCTAATTGTCATTGTTCATTTACATTCTTGAATGTTGAATTCTCTTGGTTATTCATGAACTAATGTGGAAGGTTTGTATCAATATATATGAAAACCAAACTGTTTGTTCACAATGACATTTGATGGCCTTTTGATGAATGACATGTATGCTGAAAATTTTGTTTCATTGCTTGAATTTGCTGTCTGCAGTTCATCAAATAATTGTTATGTTTTTTTTTTTCTCAGTTATATGGAGCTCCTCGGATATATAAAAAAGTTGAATCTTCTAGTGGGAGTAGACGGGTTTTTGACGATCCTCTTTTGAACTACTTCAAAGAAGTACCTGATGATCAATGGGTTAGAAGTACTGATTTTACTCCATCATCCTGTATAGGACAATCTTCTTCGTTATGTTTGAAGCTACATAACTGTCGTGAACTTCCAAATTTTGGACAGAATTTTGCTTATTACAAAGAAACTGAAGATGAATTTCGGTTCGTTGATGGAGGCAACGGTTTTTCGTGCTCTGCAGATCTTGTTCCCATGGTTGATTCTCGTCCTGATATTTTTCTGCCATATAAGATTATCTTTAAAATAAACGCATTGGTTCAACATGGTTGCATTTCAGGGCCAGCACTTGATACTAGTTTCTATCATTTGGTCGACCCGAGTAGAATAAAGATTGAATTTGTAGAACATGCCTTGGATAGACTATTCCATTTAAAGGAATGCGAGTATAAGCCATATAGTTGGCTTACTGAGCAGTACAAGAAGTATTTAAGGCGTCCTCCAAATTCTCCTGCCATATCTTTGGATGCTGGATTGGTATATGTTCGTAGGGTCCAAATAACACCTTGTAAGGTGTACTTTTGTGGTCCTGAAGTCAATGTCTCGAATCGGGTGTTGCGCCACTTTTCTCGAGATATCGATAATTTTCTTCGGGTATCTTTTGTCGATGAGGAGTGGGATAAAATGCGTTCGATAGATTTGTTGCCACGAACATCTTCTACGATTAAGGATCGTAAAACTGAAATCTACAAGAGAATTCTCTCTGTTCTTAAAAATGGCATAGTCATTGGTGGTAAAATGTTTAAGTTTCTTGCATTCTCATCAAGTCAGTTAAGAGATAATTCTTTGTGGATGTTTGCTACCAGACCTGGCCTCGACGCTGCAGATATTCGAGCGTGGATGGGAGATTTTAGACAGATCAAGAATCCAGCAAAGTATGCTGCTAGATTGGGCCAATCATTTGGCTCATCGACCGAGACGCTTTCGGTTGCTAGACATGAAAGGGAAATTATTCCTGACATAGAGGTTCAACATGGGGAAGTAAAGTATGTGTTTTCTGATGGAATTGGAAAGATATCAAGCGATTTCGCCAAAAAAGTATCTGCAAAATGTGGTTTGAAAGCATTCACCCCATCTGCTTTTCAGATTCGTTACGGCGGTTATAAGGGTGTTGTTGCTGTTGATCCATACTCATCAAAAAAATTATCTTTGAGGAAGAGCATGTGCAAATTTGAATCAGACAACACGAAACTTGACGTTTTAGGATATAGCAAATATCAACCGTGCTTCCTTAATCGTCAATTGATTACTCTTTTGTCTACTCTAGGTGTTAGAGATGAAGTTTTTGAGAAAAAGCAACACGAAGCTGTTAAACAATTGGACGCGATATTAACTGATCCTTTGAAGGCACAAGAAGCTTTAGATTTGATGGCTCCGGGAGAGAATACTAATGTCCTTAAGGAAATGCTCAAATGTGGCTATAAGCCTAATGTTGAGCCTTATCTATCAATGATGTTACAGGCTTTTCGGGCATCAAAGTTGCTAGATTTACGCACCAAATCAAGAATGTTTATTCCAAATGGGAGAGCGATGATGGGATGTCTTGACGAGACTGGGAGCTTGGAATATGGACAAGTATTTGTGCAAGTCTCAGGCACTAAGCAAAGCATTTTATCCGTCACGACCTCCGCATTCAATATGAATAGTTCGGAACAGCGTGTCGCTATCAAGGGAAATGTAGTTGTTGCAAAAAATCCGTGCCTGCACCCTGGCGACATTCGTGTATTAAAGGCTGTGAATGTGCCCCAGTTGTATCATATGGTTGATTGTGTAGTTTTTCCACAAAAAGGATCAAGGTTAGCATATTCATCTATACTAGTTCTTTTTTTATTTGAAAAATAACATCTGTTTTCAACTTTAAATGCAAGAAAAACTAATTTTTTGGCATAGTTTTGTTGCAAAGATTTTCCTTGCCAATATACATTACTAACCAGATGTTGCATTGTTAATCTAGACCTCATCCAAATGAATGCTCGGGTAGTGATTTGGATGGGGATATATACTTTGTCTGTTGGGACACTGAGCTGATCCCGCCTCGACAAATTCCACCGATGGATTATACTCCTGCACCACCTACTCAGTTGGATCGCGACGTCACAATCGAGGTATTTCCTCTCAAACCAATGGCATATTTCGATAACTTGAAAACCCATTCGAATTTCCTTTTCGTTTTTCAGTGTTTTATCTCAATGCCTACTGTAAAGAATATATTCACTTTGTAAACCATTATGATTTTGGTCCAGTAGTCAATAAGAGCAAATGCTTATAGTAAAGTCTTAGAGGGAATGAGTTCAAATCATGGTAGCCAGCTACGTATGATTAAATATCTCACAAGTTACTTTGGCAACCAAATGAAGTAGTTCAGGTAGTAAGCTATCCCAGACACTCACGGATATAAAAAAAGGAGAATATATTCACTTTGTACGTTATGTCTGACTAGAAGAGAAACCACTCATGTTTGTATGTTGTTTGTCCTAATTTGAAACTCTGCTCCTCTTTAACACCAGGATGTGCAAGAATATTTTGTGAACTACATGGTTAACGACAGTCTTGGCATCATTGCCAATGCCCACACTGCCTTTGCTGATAAAGAGCTCTTTAAAGCAAGAAGTTCTCCTTGTATAGAGCTTGCAAAGCTCTTTTCCATTGCTGTGGACTTCCCAAAAACTGGAGTACCAGCTATAATACCTCCCGAGTTATATGTCAAAGAGTTTCCCGACTTCATGGATAAGCCCGACAAACCCTCCTACGAATCGAAGAATGTGATCGGAAAGCTTTTTCGAGCAGTGAAAGACATTGCACCAACAACAAGCCATATCAGTTTGTTTACTCGAGAGGTAGCAAGGCAGTGTTACGATTCTGATATGGAAATTGAAGGCTATGAAGATTACGTCGATGATGCCATCTATTATAAGAACAATTATGATTATAAGTTGGGGAATTTGCTCGATTATTACGGGATCAAGTCTGAGGCTGAGATCCTTAGTGGAAATATAATGACTATGTCCAAGTCTTTCACCAAAAAAAGAGATGCAGAAGCCATCAACTTGGCTATCAGGTCTCTGAGAAAGGAGGCTAGGACTTGGTTCAATGAGAGAGAAAGCGGTCCCGATTCAGATTCAGTTTTGGGCTTGGATGATTTATTTGCCAAAGCTTCAGCCTGGTACCATGTCACTTATCATCCGAGTTATTGGGGAAGCTATAATGAGGATATGAACCGTGATCATTATCTGAGCTTTCCATGGTGTGTTTATGACAAGCTGATGCAGATTAAGGAGAATAATGTGAGGAGAAAACAGAGAGCTTCGAGACTAGCAGCTCTTGACAGCAGATTCAGGAATGCATTGAATCTTGGACAT

mRNA sequence

ATGGGAAAAACAGTTCATATTAGTGGATTTCCTGCAGGTGTCACGGCAGATGCCGTTAGGAGTTATTTGGAGGGTCGTACGGGTGTTGGAACTGTCTATGCTCTTAAGATTAGAGAACCCAAAAAGGGGGGAAGTAGATTATATGCTTTTGTTCAATTCACTGGCTCTAGAGAAGCTGAGTTGATCATTTCATTGGCTAATCAACGGATTCAACGCCTGTGGTATGGAACTTCGTATCTCAAGGCTCGAGAAGCGGATGTTGATATTATACCGAAGCCTAGGACGTACATGCATACCTTGGAAGATTTGACATTGTGCTTTGGTTGTCAGGTCTCAAGTGAAAAGTTTTGTGTCCTGTGGGAAGGAAGGGATGTTTTGGTGACTTTTGGATTTGGAATGCGAAAAATGAACTTTCGTTTGTCTCATAACTTTGTAGAGTACAGGTTCGAGCTTTCGTACGAGAACATTTGGCGGATAGAACTCCATCATTCGCGGCATCAGTCCATGAAGTATCTTGTGATTCAGGTTCTGGGGTCAAATTCCTTTCCAAAGTTGTCTCATGCTATACTAATGTTGAAAATTCATCAAATAATTGTTATGTTTTTTTTTTCTCAGTTATATGGAGCTCCTCGGATATATAAAAAAGTTGAATCTTCTAGTGGGAGTAGACGGGTTTTTGACGATCCTCTTTTGAACTACTTCAAAGAAGTACCTGATGATCAATGGGTTAGAAGTACTGATTTTACTCCATCATCCTGTATAGGACAATCTTCTTCGTTATGTTTGAAGCTACATAACTGTCGTGAACTTCCAAATTTTGGACAGAATTTTGCTTATTACAAAGAAACTGAAGATGAATTTCGGTTCGTTGATGGAGGCAACGGTTTTTCGTGCTCTGCAGATCTTGTTCCCATGGTTGATTCTCGTCCTGATATTTTTCTGCCATATAAGATTATCTTTAAAATAAACGCATTGGTTCAACATGGTTGCATTTCAGGGCCAGCACTTGATACTAGTTTCTATCATTTGGTCGACCCGAGTAGAATAAAGATTGAATTTGTAGAACATGCCTTGGATAGACTATTCCATTTAAAGGAATGCGAGTATAAGCCATATAGTTGGCTTACTGAGCAGTACAAGAAGTATTTAAGGCGTCCTCCAAATTCTCCTGCCATATCTTTGGATGCTGGATTGGTATATGTTCGTAGGGTCCAAATAACACCTTGTAAGGTGTACTTTTGTGGTCCTGAAGTCAATGTCTCGAATCGGGTGTTGCGCCACTTTTCTCGAGATATCGATAATTTTCTTCGGGTATCTTTTGTCGATGAGGAGTGGGATAAAATGCGTTCGATAGATTTGTTGCCACGAACATCTTCTACGATTAAGGATCGTAAAACTGAAATCTACAAGAGAATTCTCTCTGTTCTTAAAAATGGCATAGTCATTGGTGGTAAAATGTTTAAGTTTCTTGCATTCTCATCAAGTCAGTTAAGAGATAATTCTTTGTGGATGTTTGCTACCAGACCTGGCCTCGACGCTGCAGATATTCGAGCGTGGATGGGAGATTTTAGACAGATCAAGAATCCAGCAAAGTATGCTGCTAGATTGGGCCAATCATTTGGCTCATCGACCGAGACGCTTTCGGTTGCTAGACATGAAAGGGAAATTATTCCTGACATAGAGGTTCAACATGGGGAAGTAAAGTATGTGTTTTCTGATGGAATTGGAAAGATATCAAGCGATTTCGCCAAAAAAGTATCTGCAAAATGTGGTTTGAAAGCATTCACCCCATCTGCTTTTCAGATTCGTTACGGCGGTTATAAGGGTGTTGTTGCTGTTGATCCATACTCATCAAAAAAATTATCTTTGAGGAAGAGCATGTGCAAATTTGAATCAGACAACACGAAACTTGACGTTTTAGGATATAGCAAATATCAACCGTGCTTCCTTAATCGTCAATTGATTACTCTTTTGTCTACTCTAGGTGTTAGAGATGAAGTTTTTGAGAAAAAGCAACACGAAGCTGTTAAACAATTGGACGCGATATTAACTGATCCTTTGAAGGCACAAGAAGCTTTAGATTTGATGGCTCCGGGAGAGAATACTAATGTCCTTAAGGAAATGCTCAAATGTGGCTATAAGCCTAATGTTGAGCCTTATCTATCAATGATGTTACAGGCTTTTCGGGCATCAAAGTTGCTAGATTTACGCACCAAATCAAGAATGTTTATTCCAAATGGGAGAGCGATGATGGGATGTCTTGACGAGACTGGGAGCTTGGAATATGGACAAGTATTTGTGCAAGTCTCAGGCACTAAGCAAAGCATTTTATCCGTCACGACCTCCGCATTCAATATGAATAGTTCGGAACAGCGTGTCGCTATCAAGGGAAATGTAGTTGTTGCAAAAAATCCGTGCCTGCACCCTGGCGACATTCGTGTATTAAAGGCTGTGAATGTGCCCCAGTTGTATCATATGGTTGATTGTGTAGTTTTTCCACAAAAAGGATCAAGACCTCATCCAAATGAATGCTCGGGTAGTGATTTGGATGGGGATATATACTTTGTCTGTTGGGACACTGAGCTGATCCCGCCTCGACAAATTCCACCGATGGATTATACTCCTGCACCACCTACTCAGTTGGATCGCGACGTCACAATCGAGGATGTGCAAGAATATTTTGTGAACTACATGGTTAACGACAGTCTTGGCATCATTGCCAATGCCCACACTGCCTTTGCTGATAAAGAGCTCTTTAAAGCAAGAAGTTCTCCTTGTATAGAGCTTGCAAAGCTCTTTTCCATTGCTGTGGACTTCCCAAAAACTGGAGTACCAGCTATAATACCTCCCGAGTTATATGTCAAAGAGTTTCCCGACTTCATGGATAAGCCCGACAAACCCTCCTACGAATCGAAGAATGTGATCGGAAAGCTTTTTCGAGCAGTGAAAGACATTGCACCAACAACAAGCCATATCAGTTTGTTTACTCGAGAGGTAGCAAGGCAGTGTTACGATTCTGATATGGAAATTGAAGGCTATGAAGATTACGTCGATGATGCCATCTATTATAAGAACAATTATGATTATAAGTTGGGGAATTTGCTCGATTATTACGGGATCAAGTCTGAGGCTGAGATCCTTAGTGGAAATATAATGACTATGTCCAAGTCTTTCACCAAAAAAAGAGATGCAGAAGCCATCAACTTGGCTATCAGGTCTCTGAGAAAGGAGGCTAGGACTTGGTTCAATGAGAGAGAAAGCGGTCCCGATTCAGATTCAGTTTTGGGCTTGGATGATTTATTTGCCAAAGCTTCAGCCTGGTACCATGTCACTTATCATCCGAGTTATTGGGGAAGCTATAATGAGGATATGAACCGTGATCATTATCTGAGCTTTCCATGGTGTGTTTATGACAAGCTGATGCAGATTAAGGAGAATAATGTGAGGAGAAAACAGAGAGCTTCGAGACTAGCAGCTCTTGACAGCAGATTCAGGAATGCATTGAATCTTGGACAT

Coding sequence (CDS)

ATGGGAAAAACAGTTCATATTAGTGGATTTCCTGCAGGTGTCACGGCAGATGCCGTTAGGAGTTATTTGGAGGGTCGTACGGGTGTTGGAACTGTCTATGCTCTTAAGATTAGAGAACCCAAAAAGGGGGGAAGTAGATTATATGCTTTTGTTCAATTCACTGGCTCTAGAGAAGCTGAGTTGATCATTTCATTGGCTAATCAACGGATTCAACGCCTGTGGTATGGAACTTCGTATCTCAAGGCTCGAGAAGCGGATGTTGATATTATACCGAAGCCTAGGACGTACATGCATACCTTGGAAGATTTGACATTGTGCTTTGGTTGTCAGGTCTCAAGTGAAAAGTTTTGTGTCCTGTGGGAAGGAAGGGATGTTTTGGTGACTTTTGGATTTGGAATGCGAAAAATGAACTTTCGTTTGTCTCATAACTTTGTAGAGTACAGGTTCGAGCTTTCGTACGAGAACATTTGGCGGATAGAACTCCATCATTCGCGGCATCAGTCCATGAAGTATCTTGTGATTCAGGTTCTGGGGTCAAATTCCTTTCCAAAGTTGTCTCATGCTATACTAATGTTGAAAATTCATCAAATAATTGTTATGTTTTTTTTTTCTCAGTTATATGGAGCTCCTCGGATATATAAAAAAGTTGAATCTTCTAGTGGGAGTAGACGGGTTTTTGACGATCCTCTTTTGAACTACTTCAAAGAAGTACCTGATGATCAATGGGTTAGAAGTACTGATTTTACTCCATCATCCTGTATAGGACAATCTTCTTCGTTATGTTTGAAGCTACATAACTGTCGTGAACTTCCAAATTTTGGACAGAATTTTGCTTATTACAAAGAAACTGAAGATGAATTTCGGTTCGTTGATGGAGGCAACGGTTTTTCGTGCTCTGCAGATCTTGTTCCCATGGTTGATTCTCGTCCTGATATTTTTCTGCCATATAAGATTATCTTTAAAATAAACGCATTGGTTCAACATGGTTGCATTTCAGGGCCAGCACTTGATACTAGTTTCTATCATTTGGTCGACCCGAGTAGAATAAAGATTGAATTTGTAGAACATGCCTTGGATAGACTATTCCATTTAAAGGAATGCGAGTATAAGCCATATAGTTGGCTTACTGAGCAGTACAAGAAGTATTTAAGGCGTCCTCCAAATTCTCCTGCCATATCTTTGGATGCTGGATTGGTATATGTTCGTAGGGTCCAAATAACACCTTGTAAGGTGTACTTTTGTGGTCCTGAAGTCAATGTCTCGAATCGGGTGTTGCGCCACTTTTCTCGAGATATCGATAATTTTCTTCGGGTATCTTTTGTCGATGAGGAGTGGGATAAAATGCGTTCGATAGATTTGTTGCCACGAACATCTTCTACGATTAAGGATCGTAAAACTGAAATCTACAAGAGAATTCTCTCTGTTCTTAAAAATGGCATAGTCATTGGTGGTAAAATGTTTAAGTTTCTTGCATTCTCATCAAGTCAGTTAAGAGATAATTCTTTGTGGATGTTTGCTACCAGACCTGGCCTCGACGCTGCAGATATTCGAGCGTGGATGGGAGATTTTAGACAGATCAAGAATCCAGCAAAGTATGCTGCTAGATTGGGCCAATCATTTGGCTCATCGACCGAGACGCTTTCGGTTGCTAGACATGAAAGGGAAATTATTCCTGACATAGAGGTTCAACATGGGGAAGTAAAGTATGTGTTTTCTGATGGAATTGGAAAGATATCAAGCGATTTCGCCAAAAAAGTATCTGCAAAATGTGGTTTGAAAGCATTCACCCCATCTGCTTTTCAGATTCGTTACGGCGGTTATAAGGGTGTTGTTGCTGTTGATCCATACTCATCAAAAAAATTATCTTTGAGGAAGAGCATGTGCAAATTTGAATCAGACAACACGAAACTTGACGTTTTAGGATATAGCAAATATCAACCGTGCTTCCTTAATCGTCAATTGATTACTCTTTTGTCTACTCTAGGTGTTAGAGATGAAGTTTTTGAGAAAAAGCAACACGAAGCTGTTAAACAATTGGACGCGATATTAACTGATCCTTTGAAGGCACAAGAAGCTTTAGATTTGATGGCTCCGGGAGAGAATACTAATGTCCTTAAGGAAATGCTCAAATGTGGCTATAAGCCTAATGTTGAGCCTTATCTATCAATGATGTTACAGGCTTTTCGGGCATCAAAGTTGCTAGATTTACGCACCAAATCAAGAATGTTTATTCCAAATGGGAGAGCGATGATGGGATGTCTTGACGAGACTGGGAGCTTGGAATATGGACAAGTATTTGTGCAAGTCTCAGGCACTAAGCAAAGCATTTTATCCGTCACGACCTCCGCATTCAATATGAATAGTTCGGAACAGCGTGTCGCTATCAAGGGAAATGTAGTTGTTGCAAAAAATCCGTGCCTGCACCCTGGCGACATTCGTGTATTAAAGGCTGTGAATGTGCCCCAGTTGTATCATATGGTTGATTGTGTAGTTTTTCCACAAAAAGGATCAAGACCTCATCCAAATGAATGCTCGGGTAGTGATTTGGATGGGGATATATACTTTGTCTGTTGGGACACTGAGCTGATCCCGCCTCGACAAATTCCACCGATGGATTATACTCCTGCACCACCTACTCAGTTGGATCGCGACGTCACAATCGAGGATGTGCAAGAATATTTTGTGAACTACATGGTTAACGACAGTCTTGGCATCATTGCCAATGCCCACACTGCCTTTGCTGATAAAGAGCTCTTTAAAGCAAGAAGTTCTCCTTGTATAGAGCTTGCAAAGCTCTTTTCCATTGCTGTGGACTTCCCAAAAACTGGAGTACCAGCTATAATACCTCCCGAGTTATATGTCAAAGAGTTTCCCGACTTCATGGATAAGCCCGACAAACCCTCCTACGAATCGAAGAATGTGATCGGAAAGCTTTTTCGAGCAGTGAAAGACATTGCACCAACAACAAGCCATATCAGTTTGTTTACTCGAGAGGTAGCAAGGCAGTGTTACGATTCTGATATGGAAATTGAAGGCTATGAAGATTACGTCGATGATGCCATCTATTATAAGAACAATTATGATTATAAGTTGGGGAATTTGCTCGATTATTACGGGATCAAGTCTGAGGCTGAGATCCTTAGTGGAAATATAATGACTATGTCCAAGTCTTTCACCAAAAAAAGAGATGCAGAAGCCATCAACTTGGCTATCAGGTCTCTGAGAAAGGAGGCTAGGACTTGGTTCAATGAGAGAGAAAGCGGTCCCGATTCAGATTCAGTTTTGGGCTTGGATGATTTATTTGCCAAAGCTTCAGCCTGGTACCATGTCACTTATCATCCGAGTTATTGGGGAAGCTATAATGAGGATATGAACCGTGATCATTATCTGAGCTTTCCATGGTGTGTTTATGACAAGCTGATGCAGATTAAGGAGAATAATGTGAGGAGAAAACAGAGAGCTTCGAGACTAGCAGCTCTTGACAGCAGATTCAGGAATGCATTGAATCTTGGACAT

Protein sequence

MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAELIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLWEGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLGSNSFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVPDDQWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSCSADLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSRIKIEFVEHALDRLFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNVSNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGIVIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAFQIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLSTLGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGYKPNVEPYLSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTKQSILSVTTSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVNDSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIPPELYVKEFPDFMDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDAIYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEARTWFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKLMQIKENNVRRKQRASRLAALDSRFRNALNLGH
Homology
BLAST of MS020748 vs. NCBI nr
Match: XP_022146805.1 (probable RNA-dependent RNA polymerase 1 isoform X1 [Momordica charantia])

HSP 1 Score: 2278.4 bits (5903), Expect = 0.0e+00
Identity = 1124/1172 (95.90%), Postives = 1134/1172 (96.76%), Query Frame = 0

Query: 1    MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60
            MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE
Sbjct: 1    MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60

Query: 61   LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120
            LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW
Sbjct: 61   LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120

Query: 121  EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLGSN 180
            EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVI      
Sbjct: 121  EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVI------ 180

Query: 181  SFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVPDD 240
                                    QLYGAP IYKKVESSSGSR+VFDDPLLNYFKEVPDD
Sbjct: 181  ------------------------QLYGAPWIYKKVESSSGSRQVFDDPLLNYFKEVPDD 240

Query: 241  QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSCSA 300
            QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFR VDGGNGFSCSA
Sbjct: 241  QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRLVDGGNGFSCSA 300

Query: 301  DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSRIKIEFVEHALDR 360
            DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDP RIKIEFVEHALDR
Sbjct: 301  DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPRRIKIEFVEHALDR 360

Query: 361  LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420
            LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV
Sbjct: 361  LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420

Query: 421  SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI 480
            SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI
Sbjct: 421  SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI 480

Query: 481  VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPAKYAARLGQSF 540
            VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDA DIRAWMGDFRQIKNPAKYAARLGQSF
Sbjct: 481  VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAEDIRAWMGDFRQIKNPAKYAARLGQSF 540

Query: 541  GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF 600
            GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF
Sbjct: 541  GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF 600

Query: 601  QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660
            QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST
Sbjct: 601  QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660

Query: 661  LGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGYKPNVEPY 720
            LGVRDEVFEKKQ EAV+QLDAILTDPLKAQEAL+LMAPGENTNVLKEMLKCGYKPNVEPY
Sbjct: 661  LGVRDEVFEKKQREAVEQLDAILTDPLKAQEALELMAPGENTNVLKEMLKCGYKPNVEPY 720

Query: 721  LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTKQSILSVT 780
            LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSG KQSILSVT
Sbjct: 721  LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGAKQSILSVT 780

Query: 781  TSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDCVVFPQKGSRPH 840
            TSAFN++SSEQRVAI+GNVVVAKNPCLHPGD+RVLKAVNVP+LYHMVDCVVFPQKGSRPH
Sbjct: 781  TSAFNISSSEQRVAIEGNVVVAKNPCLHPGDVRVLKAVNVPRLYHMVDCVVFPQKGSRPH 840

Query: 841  PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN 900
            PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN
Sbjct: 841  PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN 900

Query: 901  DSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF 960
            DSLGIIANAHTAFADKELFKARSSPCI LAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF
Sbjct: 901  DSLGIIANAHTAFADKELFKARSSPCINLAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF 960

Query: 961  MDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA 1020
            M+KPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA
Sbjct: 961  MEKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA 1020

Query: 1021 IYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART 1080
            IY+KNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART
Sbjct: 1021 IYHKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART 1080

Query: 1081 WFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL 1140
            WFNERESGPDSDSV GLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL
Sbjct: 1081 WFNERESGPDSDSVSGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL 1140

Query: 1141 MQIKENNVRRKQRASRLAALDSRFRNALNLGH 1173
            MQIKENNVRRKQRASRLAALDSRFRNALNLGH
Sbjct: 1141 MQIKENNVRRKQRASRLAALDSRFRNALNLGH 1142

BLAST of MS020748 vs. NCBI nr
Match: XP_022146806.1 (probable RNA-dependent RNA polymerase 1 isoform X2 [Momordica charantia])

HSP 1 Score: 2191.8 bits (5678), Expect = 0.0e+00
Identity = 1091/1172 (93.09%), Postives = 1101/1172 (93.94%), Query Frame = 0

Query: 1    MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60
            MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE
Sbjct: 1    MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60

Query: 61   LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120
            LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW
Sbjct: 61   LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120

Query: 121  EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLGSN 180
            EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVI      
Sbjct: 121  EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVI------ 180

Query: 181  SFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVPDD 240
                                    QLYGAP IYKKVESSSGSR+VFDDPLLNYFKEVPDD
Sbjct: 181  ------------------------QLYGAPWIYKKVESSSGSRQVFDDPLLNYFKEVPDD 240

Query: 241  QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSCSA 300
            QW                                 NFAYYKETEDEFR VDGGNGFSCSA
Sbjct: 241  QW---------------------------------NFAYYKETEDEFRLVDGGNGFSCSA 300

Query: 301  DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSRIKIEFVEHALDR 360
            DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDP RIKIEFVEHALDR
Sbjct: 301  DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPRRIKIEFVEHALDR 360

Query: 361  LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420
            LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV
Sbjct: 361  LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420

Query: 421  SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI 480
            SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI
Sbjct: 421  SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI 480

Query: 481  VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPAKYAARLGQSF 540
            VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDA DIRAWMGDFRQIKNPAKYAARLGQSF
Sbjct: 481  VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAEDIRAWMGDFRQIKNPAKYAARLGQSF 540

Query: 541  GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF 600
            GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF
Sbjct: 541  GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF 600

Query: 601  QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660
            QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST
Sbjct: 601  QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660

Query: 661  LGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGYKPNVEPY 720
            LGVRDEVFEKKQ EAV+QLDAILTDPLKAQEAL+LMAPGENTNVLKEMLKCGYKPNVEPY
Sbjct: 661  LGVRDEVFEKKQREAVEQLDAILTDPLKAQEALELMAPGENTNVLKEMLKCGYKPNVEPY 720

Query: 721  LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTKQSILSVT 780
            LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSG KQSILSVT
Sbjct: 721  LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGAKQSILSVT 780

Query: 781  TSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDCVVFPQKGSRPH 840
            TSAFN++SSEQRVAI+GNVVVAKNPCLHPGD+RVLKAVNVP+LYHMVDCVVFPQKGSRPH
Sbjct: 781  TSAFNISSSEQRVAIEGNVVVAKNPCLHPGDVRVLKAVNVPRLYHMVDCVVFPQKGSRPH 840

Query: 841  PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN 900
            PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN
Sbjct: 841  PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN 900

Query: 901  DSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF 960
            DSLGIIANAHTAFADKELFKARSSPCI LAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF
Sbjct: 901  DSLGIIANAHTAFADKELFKARSSPCINLAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF 960

Query: 961  MDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA 1020
            M+KPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA
Sbjct: 961  MEKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA 1020

Query: 1021 IYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART 1080
            IY+KNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART
Sbjct: 1021 IYHKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART 1080

Query: 1081 WFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL 1140
            WFNERESGPDSDSV GLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL
Sbjct: 1081 WFNERESGPDSDSVSGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL 1109

Query: 1141 MQIKENNVRRKQRASRLAALDSRFRNALNLGH 1173
            MQIKENNVRRKQRASRLAALDSRFRNALNLGH
Sbjct: 1141 MQIKENNVRRKQRASRLAALDSRFRNALNLGH 1109

BLAST of MS020748 vs. NCBI nr
Match: XP_038891504.1 (RNA-dependent RNA polymerase 1 isoform X1 [Benincasa hispida] >XP_038891505.1 RNA-dependent RNA polymerase 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 1892.9 bits (4902), Expect = 0.0e+00
Identity = 934/1171 (79.76%), Postives = 1026/1171 (87.62%), Query Frame = 0

Query: 1    MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60
            MGKTVHISGFP+ VTADAV+S+LEGRTGVGTVYA+K+R PK+GG+R+YA VQFT + +AE
Sbjct: 1    MGKTVHISGFPSNVTADAVKSFLEGRTGVGTVYAIKVRAPKRGGARVYAIVQFTSATQAE 60

Query: 61   LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120
            LIISLAN   Q LWYG+SYLKAR A+VDI+PKPRTYM+TLEDL LCFGCQVSSEKF VLW
Sbjct: 61   LIISLAN---QSLWYGSSYLKARAAEVDIVPKPRTYMYTLEDLPLCFGCQVSSEKFRVLW 120

Query: 121  EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLGSN 180
            EG   LVTFG GMRKMNF L HN V+YR ELSYENIW+I+LHH +H+SMKYL+I      
Sbjct: 121  EGDIDLVTFGIGMRKMNFHLKHNSVKYRLELSYENIWQIQLHHPQHRSMKYLII------ 180

Query: 181  SFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVPDD 240
                                    QLYGAPRIYK V  +  S ++FDDPL N+F EVPDD
Sbjct: 181  ------------------------QLYGAPRIYKNV--APCSVQIFDDPLFNFFMEVPDD 240

Query: 241  QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSCSA 300
            QW+R+ DFTPS  IGQSSSLCLKL N  +LPNF QNFAYY+E E+EF+ VDG   FS   
Sbjct: 241  QWIRTADFTPSCSIGQSSSLCLKLPNRCQLPNFRQNFAYYEEFENEFQLVDGDASFSFCR 300

Query: 301  DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSRIKIEFVEHALDR 360
            DL P+VDSRP + LPY+I+FKINALVQHGCI  P +DTSFY LVDP RI+IEFVEHAL++
Sbjct: 301  DLAPIVDSRPHV-LPYEIMFKINALVQHGCIPWPLIDTSFYRLVDPRRIRIEFVEHALEK 360

Query: 361  LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420
            LFHLKEC Y+P S++TEQY+KY R PPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV
Sbjct: 361  LFHLKECSYEPSSFITEQYRKYSRHPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420

Query: 421  SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI 480
            SNRVLRHF +D DNFLRVSFVDEEWDKMRS DLLPR S T +D KT+IYKRILSVL+NGI
Sbjct: 421  SNRVLRHFPQDTDNFLRVSFVDEEWDKMRSTDLLPRMSPTSEDSKTDIYKRILSVLRNGI 480

Query: 481  VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPAKYAARLGQSF 540
            VIG K F+FLAFSSSQLRDNSLWMFA+RPGLDAADIRAWMGDFR IKNPAKYAARLGQSF
Sbjct: 481  VIGDKTFQFLAFSSSQLRDNSLWMFASRPGLDAADIRAWMGDFRHIKNPAKYAARLGQSF 540

Query: 541  GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF 600
            GSSTETLSVAR EREIIPDIE+QHGEVKYVFSDGIGKIS+DFAKKV+AKCG +  TPSAF
Sbjct: 541  GSSTETLSVARDEREIIPDIELQHGEVKYVFSDGIGKISNDFAKKVAAKCGFQGSTPSAF 600

Query: 601  QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660
            QIRYGGYKGVVAVDP+SS KLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST
Sbjct: 601  QIRYGGYKGVVAVDPHSSIKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660

Query: 661  LGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGYKPNVEPY 720
            LGVRDE+FE KQ EAV+QLDAILTDPLKA+EAL+LMAPGENTN+LKEMLKCGYKP+VEPY
Sbjct: 661  LGVRDEIFENKQREAVEQLDAILTDPLKAREALELMAPGENTNILKEMLKCGYKPDVEPY 720

Query: 721  LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTKQSILSVT 780
            LSMMLQ FRASKLL+LRTKSR+FIPNGRAMMGCLDET +LEYGQVFVQ+S  ++  LS  
Sbjct: 721  LSMMLQTFRASKLLELRTKSRIFIPNGRAMMGCLDETRTLEYGQVFVQISSARRRNLS-D 780

Query: 781  TSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDCVVFPQKGSRPH 840
            + AFNM+  E R+ I+GNV VAKNPCLHPGD+RVLKAVN+P LYH+VDCVVFPQKGSRPH
Sbjct: 781  SFAFNMSGPEHRLVIEGNVTVAKNPCLHPGDVRVLKAVNIPWLYHLVDCVVFPQKGSRPH 840

Query: 841  PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN 900
            PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPP QLDRDVT ED+QEYFVNYMVN
Sbjct: 841  PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPIQLDRDVTTEDIQEYFVNYMVN 900

Query: 901  DSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF 960
            DSLGIIANAHTAFADKE FKAR SPC+ELAKLFSIAVDFPKTGVPAIIPP LYVKEFPDF
Sbjct: 901  DSLGIIANAHTAFADKEPFKARGSPCVELAKLFSIAVDFPKTGVPAIIPPHLYVKEFPDF 960

Query: 961  MDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA 1020
            M+KPDKPSYESKNVIGKLFRAVKDIAPT S+I  FTR+VA QCYDSDME+EG+EDYV DA
Sbjct: 961  MEKPDKPSYESKNVIGKLFRAVKDIAPTLSYIRSFTRDVAMQCYDSDMEVEGFEDYVGDA 1020

Query: 1021 IYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART 1080
             Y+K+NYDYKLGNLLDYYGIKSEAEILSG+IM MSKSFT++RDAEAINLA+RSLRKEART
Sbjct: 1021 FYHKSNYDYKLGNLLDYYGIKSEAEILSGSIMRMSKSFTRRRDAEAINLAVRSLRKEART 1080

Query: 1081 WFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL 1140
            WFN RE G +SDS    DDLFAKASAWYHVTYH SYWG YNE+M RDHYLSFPWCVYDKL
Sbjct: 1081 WFNAREGGSNSDS----DDLFAKASAWYHVTYHHSYWGCYNEEMKRDHYLSFPWCVYDKL 1129

Query: 1141 MQIKENNVRRKQRASRLAALDSRFRNALNLG 1172
            MQIKE N+R+++RA RLA LD RF +ALNLG
Sbjct: 1141 MQIKEKNLRKRERALRLATLD-RFGHALNLG 1129

BLAST of MS020748 vs. NCBI nr
Match: XP_022943173.1 (RNA-dependent RNA polymerase 1-like isoform X2 [Cucurbita moschata] >XP_022943174.1 RNA-dependent RNA polymerase 1-like isoform X2 [Cucurbita moschata] >XP_022943175.1 RNA-dependent RNA polymerase 1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1892.5 bits (4901), Expect = 0.0e+00
Identity = 935/1170 (79.91%), Postives = 1031/1170 (88.12%), Query Frame = 0

Query: 1    MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60
            MGKTVHISGFP+  TAD+V+S+LE RTG+GTVYA+K+R PK+GGSR+YA VQF G+ +AE
Sbjct: 1    MGKTVHISGFPSHATADSVKSFLELRTGLGTVYAIKVRPPKRGGSRVYAIVQFIGAAQAE 60

Query: 61   LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120
            LIISLAN   QRLWYG+SYLKAR ADVDI+PKPRTY+HTLEDLTLCFGCQVSSEKF VLW
Sbjct: 61   LIISLAN---QRLWYGSSYLKARAADVDIVPKPRTYVHTLEDLTLCFGCQVSSEKFHVLW 120

Query: 121  EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLGSN 180
            EG   LVTFG  MRKMNF L HNFVEYRF+LSYENIW+I+LH  +HQSMK L+I      
Sbjct: 121  EGDVELVTFGVEMRKMNFHLIHNFVEYRFQLSYENIWQIQLHRLQHQSMKCLLI------ 180

Query: 181  SFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVPDD 240
                                    QLYGAPRIYKKV  SSG  ++FDDPL N+FKEVPDD
Sbjct: 181  ------------------------QLYGAPRIYKKVAPSSG--QIFDDPLFNFFKEVPDD 240

Query: 241  QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSCSA 300
            QWVR+ DFTPS+ +GQSSSLCLKL N R+LPNF ++FAYY+E EDEF+ VDGGN FSCS 
Sbjct: 241  QWVRTADFTPSNSVGQSSSLCLKLPNGRQLPNFRRHFAYYEEFEDEFKLVDGGNSFSCSD 300

Query: 301  DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSRIKIEFVEHALDR 360
            DLVP+VDSRP++ LPY+IIFK+N LVQ+GCI+GP+LDT FY LVDPSRI++E+VE+AL++
Sbjct: 301  DLVPVVDSRPNVLLPYEIIFKVNVLVQNGCIAGPSLDTGFYRLVDPSRIRVEYVENALEK 360

Query: 361  LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420
            LFHLKEC Y+P S+LTEQYKKY + P NSPAI+LD GLVYVRRVQITPCKVYFCGPEVNV
Sbjct: 361  LFHLKECCYEPSSFLTEQYKKYAKHPSNSPAIALDDGLVYVRRVQITPCKVYFCGPEVNV 420

Query: 421  SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI 480
            SNRVLRHFSRD+DNFLRVSFVDEEWDKMRS DLLPR +S+I++ KT+IY+RILSVLKNGI
Sbjct: 421  SNRVLRHFSRDLDNFLRVSFVDEEWDKMRSTDLLPRMASSIENGKTDIYRRILSVLKNGI 480

Query: 481  VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPAKYAARLGQSF 540
             IG K FKFLAFSSSQLR+NSLWMFA+RPGLDAA IR WMGDFR IKNPAKYAARLGQSF
Sbjct: 481  AIGDKKFKFLAFSSSQLRENSLWMFASRPGLDAAVIREWMGDFRHIKNPAKYAARLGQSF 540

Query: 541  GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF 600
            GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAK+V++KC L AFTPSAF
Sbjct: 541  GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKRVASKCVL-AFTPSAF 600

Query: 601  QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660
            QIRYGGYKGVVAVDP SS KLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST
Sbjct: 601  QIRYGGYKGVVAVDPRSSVKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660

Query: 661  LGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGYKPNVEPY 720
            LGV+DEVFEKKQ EA++QLDAILTDPLKAQEAL+LM+PGENTN+LKEMLKCGYKP+VEPY
Sbjct: 661  LGVKDEVFEKKQSEALEQLDAILTDPLKAQEALELMSPGENTNILKEMLKCGYKPDVEPY 720

Query: 721  LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTKQSILSVT 780
            LSMMLQ FR SKLL+LRTKSR+FIPNGRAMMGCLDETG+LEYGQVFVQ+S  +   +S  
Sbjct: 721  LSMMLQTFRESKLLELRTKSRIFIPNGRAMMGCLDETGTLEYGQVFVQISSVRHRHIS-D 780

Query: 781  TSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDCVVFPQKGSRPH 840
            TSAF+M+ SE  + I GNV VAKNPCLHPGD+RVL AVNVP LYHMVDCVVFPQKGSRPH
Sbjct: 781  TSAFDMSGSEDHLVIVGNVTVAKNPCLHPGDVRVLNAVNVPLLYHMVDCVVFPQKGSRPH 840

Query: 841  PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN 900
            PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPP QLDRDVTIEDVQEYFVNYMVN
Sbjct: 841  PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPIQLDRDVTIEDVQEYFVNYMVN 900

Query: 901  DSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF 960
            DSLGIIANAHTAFADKE FKARS+PCIELAKLFSIAVDFPKTGVPAIIP  L VKEFPDF
Sbjct: 901  DSLGIIANAHTAFADKEPFKARSAPCIELAKLFSIAVDFPKTGVPAIIPSHLNVKEFPDF 960

Query: 961  MDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA 1020
            M+KPD+PSYESKNVIGKLFRAVK IAP  S+I  FTR+VA +CYDSDME EG+EDYV+DA
Sbjct: 961  MEKPDRPSYESKNVIGKLFRAVKGIAPNISYIRSFTRDVAMRCYDSDMETEGFEDYVEDA 1020

Query: 1021 IYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART 1080
            IY+K  YDYKLGNLLDYYGIKSE+EI SGNIM MSKSFT++RDAEAINLA RSLRKEART
Sbjct: 1021 IYHKTIYDYKLGNLLDYYGIKSESEIFSGNIMRMSKSFTRRRDAEAINLAARSLRKEART 1080

Query: 1081 WFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL 1140
            WFN +ESGPDS    G DDLFAKASAWYHVTYH SYWGSYN+ M RDHYLSFPWCVY KL
Sbjct: 1081 WFNTKESGPDS----GSDDLFAKASAWYHVTYHHSYWGSYNDGMKRDHYLSFPWCVYHKL 1128

Query: 1141 MQIKENNVRRKQRASRLAALDSRFRNALNL 1171
            MQ+KENN+RR+++ASRLA LD RF   LNL
Sbjct: 1141 MQVKENNLRRREKASRLATLD-RFSRVLNL 1128

BLAST of MS020748 vs. NCBI nr
Match: XP_022943172.1 (RNA-dependent RNA polymerase 1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1892.5 bits (4901), Expect = 0.0e+00
Identity = 935/1170 (79.91%), Postives = 1031/1170 (88.12%), Query Frame = 0

Query: 1    MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60
            MGKTVHISGFP+  TAD+V+S+LE RTG+GTVYA+K+R PK+GGSR+YA VQF G+ +AE
Sbjct: 15   MGKTVHISGFPSHATADSVKSFLELRTGLGTVYAIKVRPPKRGGSRVYAIVQFIGAAQAE 74

Query: 61   LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120
            LIISLAN   QRLWYG+SYLKAR ADVDI+PKPRTY+HTLEDLTLCFGCQVSSEKF VLW
Sbjct: 75   LIISLAN---QRLWYGSSYLKARAADVDIVPKPRTYVHTLEDLTLCFGCQVSSEKFHVLW 134

Query: 121  EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLGSN 180
            EG   LVTFG  MRKMNF L HNFVEYRF+LSYENIW+I+LH  +HQSMK L+I      
Sbjct: 135  EGDVELVTFGVEMRKMNFHLIHNFVEYRFQLSYENIWQIQLHRLQHQSMKCLLI------ 194

Query: 181  SFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVPDD 240
                                    QLYGAPRIYKKV  SSG  ++FDDPL N+FKEVPDD
Sbjct: 195  ------------------------QLYGAPRIYKKVAPSSG--QIFDDPLFNFFKEVPDD 254

Query: 241  QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSCSA 300
            QWVR+ DFTPS+ +GQSSSLCLKL N R+LPNF ++FAYY+E EDEF+ VDGGN FSCS 
Sbjct: 255  QWVRTADFTPSNSVGQSSSLCLKLPNGRQLPNFRRHFAYYEEFEDEFKLVDGGNSFSCSD 314

Query: 301  DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSRIKIEFVEHALDR 360
            DLVP+VDSRP++ LPY+IIFK+N LVQ+GCI+GP+LDT FY LVDPSRI++E+VE+AL++
Sbjct: 315  DLVPVVDSRPNVLLPYEIIFKVNVLVQNGCIAGPSLDTGFYRLVDPSRIRVEYVENALEK 374

Query: 361  LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420
            LFHLKEC Y+P S+LTEQYKKY + P NSPAI+LD GLVYVRRVQITPCKVYFCGPEVNV
Sbjct: 375  LFHLKECCYEPSSFLTEQYKKYAKHPSNSPAIALDDGLVYVRRVQITPCKVYFCGPEVNV 434

Query: 421  SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI 480
            SNRVLRHFSRD+DNFLRVSFVDEEWDKMRS DLLPR +S+I++ KT+IY+RILSVLKNGI
Sbjct: 435  SNRVLRHFSRDLDNFLRVSFVDEEWDKMRSTDLLPRMASSIENGKTDIYRRILSVLKNGI 494

Query: 481  VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPAKYAARLGQSF 540
             IG K FKFLAFSSSQLR+NSLWMFA+RPGLDAA IR WMGDFR IKNPAKYAARLGQSF
Sbjct: 495  AIGDKKFKFLAFSSSQLRENSLWMFASRPGLDAAVIREWMGDFRHIKNPAKYAARLGQSF 554

Query: 541  GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF 600
            GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAK+V++KC L AFTPSAF
Sbjct: 555  GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKRVASKCVL-AFTPSAF 614

Query: 601  QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660
            QIRYGGYKGVVAVDP SS KLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST
Sbjct: 615  QIRYGGYKGVVAVDPRSSVKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 674

Query: 661  LGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGYKPNVEPY 720
            LGV+DEVFEKKQ EA++QLDAILTDPLKAQEAL+LM+PGENTN+LKEMLKCGYKP+VEPY
Sbjct: 675  LGVKDEVFEKKQSEALEQLDAILTDPLKAQEALELMSPGENTNILKEMLKCGYKPDVEPY 734

Query: 721  LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTKQSILSVT 780
            LSMMLQ FR SKLL+LRTKSR+FIPNGRAMMGCLDETG+LEYGQVFVQ+S  +   +S  
Sbjct: 735  LSMMLQTFRESKLLELRTKSRIFIPNGRAMMGCLDETGTLEYGQVFVQISSVRHRHIS-D 794

Query: 781  TSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDCVVFPQKGSRPH 840
            TSAF+M+ SE  + I GNV VAKNPCLHPGD+RVL AVNVP LYHMVDCVVFPQKGSRPH
Sbjct: 795  TSAFDMSGSEDHLVIVGNVTVAKNPCLHPGDVRVLNAVNVPLLYHMVDCVVFPQKGSRPH 854

Query: 841  PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN 900
            PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPP QLDRDVTIEDVQEYFVNYMVN
Sbjct: 855  PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPIQLDRDVTIEDVQEYFVNYMVN 914

Query: 901  DSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF 960
            DSLGIIANAHTAFADKE FKARS+PCIELAKLFSIAVDFPKTGVPAIIP  L VKEFPDF
Sbjct: 915  DSLGIIANAHTAFADKEPFKARSAPCIELAKLFSIAVDFPKTGVPAIIPSHLNVKEFPDF 974

Query: 961  MDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA 1020
            M+KPD+PSYESKNVIGKLFRAVK IAP  S+I  FTR+VA +CYDSDME EG+EDYV+DA
Sbjct: 975  MEKPDRPSYESKNVIGKLFRAVKGIAPNISYIRSFTRDVAMRCYDSDMETEGFEDYVEDA 1034

Query: 1021 IYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART 1080
            IY+K  YDYKLGNLLDYYGIKSE+EI SGNIM MSKSFT++RDAEAINLA RSLRKEART
Sbjct: 1035 IYHKTIYDYKLGNLLDYYGIKSESEIFSGNIMRMSKSFTRRRDAEAINLAARSLRKEART 1094

Query: 1081 WFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL 1140
            WFN +ESGPDS    G DDLFAKASAWYHVTYH SYWGSYN+ M RDHYLSFPWCVY KL
Sbjct: 1095 WFNTKESGPDS----GSDDLFAKASAWYHVTYHHSYWGSYNDGMKRDHYLSFPWCVYHKL 1142

Query: 1141 MQIKENNVRRKQRASRLAALDSRFRNALNL 1171
            MQ+KENN+RR+++ASRLA LD RF   LNL
Sbjct: 1155 MQVKENNLRRREKASRLATLD-RFSRVLNL 1142

BLAST of MS020748 vs. ExPASy Swiss-Prot
Match: Q9LQV2 (RNA-dependent RNA polymerase 1 OS=Arabidopsis thaliana OX=3702 GN=RDR1 PE=2 SV=1)

HSP 1 Score: 1407.5 bits (3642), Expect = 0.0e+00
Identity = 711/1168 (60.87%), Postives = 871/1168 (74.57%), Query Frame = 0

Query: 1    MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60
            MGKT+ + GFP GV+A+ V+ +LE  TG GTVYA+K+R+PKKGG R+YA VQFT  R   
Sbjct: 1    MGKTIQVFGFPNGVSAEEVKKFLERLTGSGTVYAIKVRQPKKGGPRVYAIVQFTSERHTR 60

Query: 61   LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120
            LII+ A    +RL+YG SYLKA E + DI+PKPR  +HT+  L + FGCQVS++KF  LW
Sbjct: 61   LIITAA---AERLYYGRSYLKAFEVEQDIVPKPRASLHTISGLKMFFGCQVSTKKFLTLW 120

Query: 121  EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLGSN 180
              +DV V+FG GMRK++F  S    +YR ELSYENIW+I+LH  + +S K+LVIQV+   
Sbjct: 121  SAQDVCVSFGIGMRKLHFSFSWYQKDYRLELSYENIWQIDLHSPQGRSSKFLVIQVI--- 180

Query: 181  SFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVPDD 240
                                       GAP+I++K +       +F   +++++ +  D+
Sbjct: 181  ---------------------------GAPKIFEKEDQP--INLLFG--IMDFYSDGSDE 240

Query: 241  QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSCSA 300
            QW+R+TDFT SSCIGQS++ CL+L     +P+F +NFA Y E       ++ G+ +S +A
Sbjct: 241  QWIRTTDFTSSSCIGQSTAFCLELPVHLNVPDFRENFANYAEHRASSFLIESGSSYSSNA 300

Query: 301  D-LVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSRIKIEFVEHALD 360
            + LVP+VD  P   LP++I+FK+N LVQ+ C+SGPALD  FY L++  +     ++H L+
Sbjct: 301  NTLVPVVDPPPGFSLPFEILFKLNTLVQNACLSGPALDLDFYRLLNQKKYDRALIDHCLE 360

Query: 361  RLFHLKECEYKPYSWLTEQYKKYLRRP--PNSPAISLDAGLVYVRRVQITPCKVYFCGPE 420
            +LFHL EC Y+P  WL ++YKK++ +   P SP ISLD GLVY+ RVQ+TP +VYF GPE
Sbjct: 361  KLFHLGECCYEPAHWLRDEYKKWISKGKLPLSPTISLDDGLVYMYRVQVTPARVYFSGPE 420

Query: 421  VNVSNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLK 480
            VNVSNRVLRH+S+ I+NFLRVSFVDE+ +K+RS+DL PR+S+    R+T++Y RI SVL+
Sbjct: 421  VNVSNRVLRHYSKYINNFLRVSFVDEDLEKVRSMDLSPRSST---QRRTKLYDRIYSVLR 480

Query: 481  NGIVIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPAKYAARLG 540
            +GIVIG K F+FLAFSSSQLR+NS WMFA    + AA IRAWMGDF  I+N AKYAARLG
Sbjct: 481  DGIVIGDKKFEFLAFSSSQLRENSAWMFAPIDRITAAHIRAWMGDFDHIRNVAKYAARLG 540

Query: 541  QSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTP 600
            QSF SS ETL+V   E E+IPD+E+     +YVFSDGIGKIS++FA+KV+ KCGL  F+P
Sbjct: 541  QSFSSSRETLNVRSDEIEVIPDVEIISLGTRYVFSDGIGKISAEFARKVARKCGLTEFSP 600

Query: 601  SAFQIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITL 660
            SAFQIRYGGYKGVVAVDP SSKKLSLRKSM KFES+NTKLDVL +SKYQPC++NRQLITL
Sbjct: 601  SAFQIRYGGYKGVVAVDPNSSKKLSLRKSMSKFESENTKLDVLAWSKYQPCYMNRQLITL 660

Query: 661  LSTLGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGYKPNV 720
            LSTLGV D VFEKKQ E V +LDAILT PL+A EAL LMAPGENTN+LK ++ CGYKP+ 
Sbjct: 661  LSTLGVTDSVFEKKQREVVDRLDAILTHPLEAHEALGLMAPGENTNILKALILCGYKPDA 720

Query: 721  EPYLSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTKQSIL 780
            EP+LSMMLQ FRASKLL+LRTK+R+FI  GR+MMGCLDET +LEYGQV VQ S   +   
Sbjct: 721  EPFLSMMLQNFRASKLLELRTKTRIFISGGRSMMGCLDETRTLEYGQVVVQYSDPMR--- 780

Query: 781  SVTTSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDCVVFPQKGS 840
                         +R  I G VVVAKNPCLHPGD+RVL+AVNVP L HMVDCVVFPQKG 
Sbjct: 781  -----------PGRRFIITGPVVVAKNPCLHPGDVRVLQAVNVPALNHMVDCVVFPQKGL 840

Query: 841  RPHPNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNY 900
            RPHPNECSGSDLDGDIYFVCWD EL+PPR   PMDYTP P   LD DVTIE+V+EYF NY
Sbjct: 841  RPHPNECSGSDLDGDIYFVCWDQELVPPRTSEPMDYTPEPTQILDHDVTIEEVEEYFANY 900

Query: 901  MVNDSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIPPELYVKEF 960
            +VNDSLGIIANAHTAFADKE  KA S PCIELAK FS AVDFPKTGV A+IP  LYVKE+
Sbjct: 901  IVNDSLGIIANAHTAFADKEPLKAFSDPCIELAKKFSTAVDFPKTGVAAVIPQHLYVKEY 960

Query: 961  PDFMDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYV 1020
            PDFM+KPDKP+YESKNVIGKLFR VK+ AP    I  FT +VA + YD DME++G+E+YV
Sbjct: 961  PDFMEKPDKPTYESKNVIGKLFREVKERAPPLISIKSFTLDVASKSYDKDMEVDGFEEYV 1020

Query: 1021 DDAIYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKE 1080
            D+A Y K NYD+KLGNL+DYYGIK+EAEILSG IM MSKSFTK+RDAE+I  A+R+LRKE
Sbjct: 1021 DEAFYQKANYDFKLGNLMDYYGIKTEAEILSGGIMRMSKSFTKRRDAESIGRAVRALRKE 1080

Query: 1081 ARTWFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVY 1140
              + FN  E   +           AKASAWYHVTYH SYWG YNE +NRDH+LSF WCVY
Sbjct: 1081 TLSLFNASEEEENES---------AKASAWYHVTYHSSYWGLYNEGLNRDHFLSFAWCVY 1105

Query: 1141 DKLMQIKENNVRRKQRASRLAALDSRFR 1166
            DKL++IK+ N+ R+QR   L  LD   R
Sbjct: 1141 DKLVRIKKTNLGRRQRQETLERLDHVLR 1105

BLAST of MS020748 vs. ExPASy Swiss-Prot
Match: Q0DXS3 (Probable RNA-dependent RNA polymerase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=RDR1 PE=2 SV=2)

HSP 1 Score: 987.3 bits (2551), Expect = 1.5e-286
Identity = 497/744 (66.80%), Postives = 592/744 (79.57%), Query Frame = 0

Query: 411  VYFCGPEVNVSNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYK 470
            VYF GPE+NVSNRV+R+FS DI+NFLR+SFVDE+ +K+R+ DL PR++S     +T +YK
Sbjct: 9    VYFYGPEINVSNRVVRNFSSDIENFLRISFVDEDCEKLRATDLSPRSASGHDANRTALYK 68

Query: 471  RILSVLKNGIVIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPA 530
            R+LSVL +GI IGGK F+FLAFSSSQLRDNS WMFA+R GL A+DIR WMGDFR I+N A
Sbjct: 69   RVLSVLSDGITIGGKNFEFLAFSSSQLRDNSAWMFASRQGLAASDIRTWMGDFRNIRNVA 128

Query: 531  KYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKC 590
            KYAARLGQSF SSTETL V ++E E I DI+      ++VFSDGIGKISS FA +V+ KC
Sbjct: 129  KYAARLGQSFSSSTETLKVQKYEVEEISDIK---NGTQHVFSDGIGKISSAFANEVAMKC 188

Query: 591  GLKAFTPSAFQIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFL 650
             LK F PSAFQIRYGGYKGVVAVDP S  KLSLRKSM KF+SDN  +DVL YSKYQP FL
Sbjct: 189  NLKRFAPSAFQIRYGGYKGVVAVDPTSRWKLSLRKSMLKFQSDNITVDVLAYSKYQPGFL 248

Query: 651  NRQLITLLSTLGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLK 710
            NRQLITLLSTLGVRD VFE+KQ EAV QL+ ++TDP  A EA++LM  GE TN +KE+L 
Sbjct: 249  NRQLITLLSTLGVRDSVFEQKQEEAVNQLNKMVTDPQAAIEAIELMPMGEITNAVKELLL 308

Query: 711  CGYKPNVEPYLSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVS 770
            CGY+P+ EPYLSM+LQ FRASKLL+L+TKSR+ IP GRAMMGCLDET +L+YGQVF++  
Sbjct: 309  CGYQPDDEPYLSMLLQTFRASKLLELKTKSRILIPKGRAMMGCLDETRTLKYGQVFIR-- 368

Query: 771  GTKQSILSVTTSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDCV 830
                      TS  N N    R  + G VV+AKNPCLHPGDIR+L AV+VP L+HM +CV
Sbjct: 369  ---------ATSGVNDN---DRFTVTGKVVIAKNPCLHPGDIRILHAVDVPVLHHMFNCV 428

Query: 831  VFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDV 890
            VFPQ+G RPHPNECSGSDLDGDIYFV WD  LIPPR + PMDYTPAP   LD DVTIE+V
Sbjct: 429  VFPQQGPRPHPNECSGSDLDGDIYFVSWDPSLIPPRMVTPMDYTPAPTETLDHDVTIEEV 488

Query: 891  QEYFVNYMVNDSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIPP 950
            +EYF NY+VN+SLG+IANAH  FADKE  KA SSPCIELAKLFSIAVDFPKTGVPA+IPP
Sbjct: 489  EEYFTNYIVNESLGMIANAHVVFADKEDLKAESSPCIELAKLFSIAVDFPKTGVPALIPP 548

Query: 951  ELYVKEFPDFMDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEI 1010
            EL+VKE+PDFM+K DK +YESK VIGKL+R +K   P   HI  FTREVAR+ YD+DM +
Sbjct: 549  ELHVKEYPDFMEKLDKVTYESKGVIGKLYREIKKHTP---HIKHFTREVARRSYDTDMIV 608

Query: 1011 EGYEDYVDDAIYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLA 1070
            +GYEDY+ +A+  K+ YD+KLGNL+D+YGIKSEAEI+SG I+ M+K+FTKK DA+AI LA
Sbjct: 609  DGYEDYITEAMALKDEYDFKLGNLMDHYGIKSEAEIISGCILKMAKNFTKKSDADAIRLA 668

Query: 1071 IRSLRKEARTWFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYL 1130
            +RSLRKEAR+ F+E       D+  G D   AKASAWYHVTYHP +WG YNE   R H++
Sbjct: 669  VRSLRKEARSRFSEMSL---DDNGHGHDASEAKASAWYHVTYHPEFWGCYNEGYERPHFI 728

Query: 1131 SFPWCVYDKLMQIKENN--VRRKQ 1153
            SFPWC+Y+KL++IK+    VR+ Q
Sbjct: 729  SFPWCIYEKLLRIKQRRKFVRKMQ 729

BLAST of MS020748 vs. ExPASy Swiss-Prot
Match: O82504 (RNA-dependent RNA polymerase 2 OS=Arabidopsis thaliana OX=3702 GN=RDR2 PE=1 SV=1)

HSP 1 Score: 758.4 bits (1957), Expect = 1.2e-217
Identity = 466/1181 (39.46%), Postives = 678/1181 (57.41%), Query Frame = 0

Query: 4    TVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGS-RLYAFVQFTGSREAELI 63
            TV IS  P  + AD +  +LE   G  TV+AL+I   +     R +A VQFT        
Sbjct: 11   TVKISNVPQTIVADELLRFLELHLGEDTVFALEIPTTRDNWKPRDFARVQFTTLEVKSRA 70

Query: 64   ISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEK-FCVL-- 123
              L++Q   +L + T  L+  EA  DIIP+P      L+D+ L  G   S EK FC L  
Sbjct: 71   QLLSSQ--SKLLFKTHNLRLSEAYDDIIPRPVDPRKRLDDIVLTVGFPESDEKRFCALEK 130

Query: 124  WEG-RDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLG 183
            W+G R  ++T     R++ F +  +   Y+ E+ +E+I            ++ L   V G
Sbjct: 131  WDGVRCWILT---EKRRVEFWVWESGDCYKIEVRFEDI------------IETLSCCVNG 190

Query: 184  SNSFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVP 243
              S  ++   +L LK             YG P+++K+V     ++  F      + KE  
Sbjct: 191  DAS--EIDAFLLKLK-------------YG-PKVFKRVTVHIATK--FKSDRYRFCKEDF 250

Query: 244  DDQWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSC 303
            D  W+R+TDF+ S  IG S+  CL++HN   + +      YY+E      +VD G  F+ 
Sbjct: 251  DFMWIRTTDFSGSKSIGTSTCFCLEVHNGSTMLDIFSGLPYYREDTLSLTYVD-GKTFAS 310

Query: 304  SADLVPMVDSR-PDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSR-IKIEFVEH 363
            +A +VP++++    +  PY+I+F++NALV    IS      S   L+   R + +E    
Sbjct: 311  AAQIVPLLNAAILGLEFPYEILFQLNALVHAQKIS--LFAASDMELIKILRGMSLETALV 370

Query: 364  ALDRLFHLKECEYKPYSWLTEQYKKYLRRPPNSPAIS----LDAGLVYVRRVQITPCKVY 423
             L +L       Y P  ++  Q +  +++  +SPA +     +  ++  +R  +TP K+Y
Sbjct: 371  ILKKLHQQSSICYDPVFFVKTQMQSVVKKMKHSPASAYKRLTEQNIMSCQRAYVTPSKIY 430

Query: 424  FCGPEVNVSNRVLRHFSRDIDNFLRVSFVDEEWDKM--RSIDLLPRTSSTIKDRKTEIYK 483
              GPE+  +N V+++F+  + +F+RV+FV+E+W K+   ++ +  +    +K  +T IY 
Sbjct: 431  LLGPELETANYVVKNFAEHVSDFMRVTFVEEDWSKLPANALSVNSKEGYFVKPSRTNIYN 490

Query: 484  RILSVLKNGIVIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPA 543
            R+LS+L  GI +G K F+FLAFS+SQLR NS+WMFA+   + A DIR WMG FR+I++ +
Sbjct: 491  RVLSILGEGITVGPKRFEFLAFSASQLRGNSVWMFASNEKVKAEDIREWMGCFRKIRSIS 550

Query: 544  KYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKC 603
            K AAR+GQ F +S +TL V   + E IPDIEV      Y FSDGIGKIS  FAK+V+ KC
Sbjct: 551  KCAARMGQLFSASRQTLIVRAQDVEQIPDIEVTTDGADYCFSDGIGKISLAFAKQVAQKC 610

Query: 604  GLKAFTPSAFQIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFL 663
            GL +  PSAFQIRYGGYKGV+AVD  S +KLSLR SM KF+S+N  L+V  +++  PCFL
Sbjct: 611  GL-SHVPSAFQIRYGGYKGVIAVDRSSFRKLSLRDSMLKFDSNNRMLNVTRWTESMPCFL 670

Query: 664  NRQLITLLSTLGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLK 723
            NR++I LLSTLG+ D +FE  Q   +  L  +L D   A   L  ++   + N+L +ML 
Sbjct: 671  NREIICLLSTLGIEDAMFEAMQAVHLSMLGNMLEDRDAALNVLQKLSGENSKNLLVKMLL 730

Query: 724  CGYKPNVEPYLSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVS 783
             GY P+ EPYLSMML+    S+L +L+++ R+ +P GR ++GC+DE G LEYGQV+V+V+
Sbjct: 731  QGYAPSSEPYLSMMLRVHHESQLSELKSRCRILVPKGRILIGCMDEMGILEYGQVYVRVT 790

Query: 784  GTKQSILSVTTSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYH--MVD 843
             TK  + S   S F     E  V I G VVV KNPCLHPGDIRVL A+          +D
Sbjct: 791  LTKAELKSRDQSYFRKIDEETSVVI-GKVVVTKNPCLHPGDIRVLDAIYEVHFEEKGYLD 850

Query: 844  CVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIE 903
            C++FPQKG RPHPNECSG DLDGD +FV WD ++IP    PPMDY  + P  +D DVT+E
Sbjct: 851  CIIFPQKGERPHPNECSGGDLDGDQFFVSWDEKIIPSEMDPPMDYAGSRPRLMDHDVTLE 910

Query: 904  DVQEYFVNYMVNDSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAII 963
            ++ ++FV+YM++D+LG+I+ AH   AD++  KARS  C+ELA L S AVDF KTG PA +
Sbjct: 911  EIHKFFVDYMISDTLGVISTAHLVHADRDPEKARSQKCLELANLHSRAVDFAKTGAPAEM 970

Query: 964  PPELYVKEFPDFMDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDM 1023
            P  L  +EFPDF+++ +KP+Y S++V GKL+RAVK         +     VA   YD  +
Sbjct: 971  PYALKPREFPDFLERFEKPTYISESVFGKLYRAVKSSLAQRKPEAESEDTVA---YDVTL 1030

Query: 1024 EIEGYEDYVDDAIYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRD----- 1083
            E  G+E +++ A  +++ Y  KL +L+ YYG  +E EIL+G + T  K     RD     
Sbjct: 1031 EEAGFESFIETAKAHRDMYGEKLTSLMIYYGAANEEEILTGILKT--KEMYLARDNRRYG 1090

Query: 1084 --AEAINLAIRSLRKEARTWFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYN 1143
               + I L+++ L KEA  WF +          L        ASAWY+VTY+P      N
Sbjct: 1091 DMKDRITLSVKDLHKEAMGWFEKSCEDEQQKKKL--------ASAWYYVTYNP------N 1132

Query: 1144 EDMNRDHYLSFPWCVYDKLMQIKENNVRRKQRASRLAALDS 1163
                +  +LSFPW V D L+ IK  N +R+    + + L S
Sbjct: 1151 HRDEKLTFLSFPWIVGDVLLDIKAENAQRQSVEEKTSGLVS 1132

BLAST of MS020748 vs. ExPASy Swiss-Prot
Match: Q7XM31 (Probable RNA-dependent RNA polymerase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=RDR2 PE=2 SV=1)

HSP 1 Score: 720.7 bits (1859), Expect = 2.7e-206
Identity = 434/1173 (37.00%), Postives = 653/1173 (55.67%), Query Frame = 0

Query: 4    TVHISGFPAGVTADAVRSYLEGRTGV-GTVYALKIREPKKGG-SRLYAFVQFTGSREAEL 63
            ++ +S  P    A  + ++ +    V G  +A +I    +G  SR +  VQF  +  A  
Sbjct: 11   SLRVSNIPPSAVAAELLAFFDSAVAVAGGAFACEIAAAHRGWLSRGHGTVQFGSAAAAAA 70

Query: 64   IISLANQ-RIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKF--CV 123
               LA+  R+ R  +  + L    + VD++P+            L  G +V+   F    
Sbjct: 71   AAGLASSGRLPR--FLGALLSVSPSPVDLLPRASDLSLRAAGAGLVVGDRVAERVFEAAD 130

Query: 124  LWEGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLG 183
             W+G  V      G R+++  L H+   Y+ E+ +E++          +      +  +G
Sbjct: 131  AWDG--VRAEVIPGKRRVDLYLEHDSQRYKLEVLFEDM----------KDCLGCTLDGMG 190

Query: 184  SNSFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVP 243
                     AIL+             QL  APRI+  +   + + R  DD   +  KE  
Sbjct: 191  ---------AILL-------------QLNYAPRIHTAISGPAVNSRFMDD-RFHACKEDA 250

Query: 244  DDQWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSC 303
               WVR+ DFTP+   G+ S+L LKL     + +  ++  +     +    ++  +G   
Sbjct: 251  KFSWVRALDFTPNYSFGRCSTLVLKLGKSALVSDILKSLPFSGNLGE--LTMNSMDGVGA 310

Query: 304  SADLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSRIKIEFVEHAL 363
            S+++VP+V    D  +PY+++F++N+L+  G I    ++   +  +    + ++      
Sbjct: 311  SSNVVPLVHCPRDYSVPYEVLFRLNSLMHMGKIVAKHVNADLFKAL--QELPVDVSRRIF 370

Query: 364  DRLFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDA------GLVYVRRVQITPCKVY 423
            +++  L+   Y P  ++  Q + Y  +  ++  +S +        L+   RV ITP K++
Sbjct: 371  EKMHKLESTCYGPLQFI--QQEAYSMKRSHNVLLSNEGEGEGERKLMKCYRVNITPSKIF 430

Query: 424  FCGPEVNVSNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTI--KDRKTEIYK 483
              GPE  V+N V++H S    +F+RV+FVDE+W K+ S  +  R       K  KT +Y 
Sbjct: 431  CFGPEEEVTNYVVKHHSAYASDFVRVTFVDEDWSKLSSNAISARIEQGFFSKPFKTGLYY 490

Query: 484  RILSVLKNGIVIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPA 543
            RILS+LK G  IG K F+FLAFS+SQLR NS+WMFA+   L+A  IR WMG F  I++ +
Sbjct: 491  RILSILKKGFSIGPKNFEFLAFSASQLRGNSVWMFASNASLNAGGIRRWMGHFENIRSVS 550

Query: 544  KYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKC 603
            K AAR+GQ F SS +T  V R + E+IPDIE+     KY+FSDGIGKIS  FAK+V+   
Sbjct: 551  KCAARMGQLFSSSRQTFEVLRWDVEVIPDIEITTDGSKYIFSDGIGKISLRFAKRVAHHV 610

Query: 604  GLKAFT-PSAFQIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCF 663
            GL     PSAFQIRYGGYKGV+A+DP SS  LSLR SM KFES++  L++  +SK QPC+
Sbjct: 611  GLDPTNLPSAFQIRYGGYKGVIAIDPMSSIDLSLRPSMKKFESESRMLNITSWSKSQPCY 670

Query: 664  LNRQLITLLSTLGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEML 723
            +NR++I+LLSTLG+RDE+F   Q + +++ + +LT+   A   L  +  G  T    +ML
Sbjct: 671  VNREIISLLSTLGIRDEIFVAMQQDEMRETEEMLTNKEVALSVLGKLG-GSETKTAVKML 730

Query: 724  KCGYKPNVEPYLSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQV 783
              GY+P+ EPYLSM+L+A + ++L D+RT+ ++ +P GR ++GCLDETG LEYGQV++++
Sbjct: 731  LQGYEPSSEPYLSMILKAHQENRLTDIRTRCKIHVPKGRVLIGCLDETGVLEYGQVYIRI 790

Query: 784  SGTKQSILSVTTSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDC 843
            +   +       S F  N   +   + G V + KNPCLHPGDIRVL+A+  P L  MVDC
Sbjct: 791  TKNSKEQKDSNQSYF-YNDDGKTATVVGKVAITKNPCLHPGDIRVLEAIYDPDLVGMVDC 850

Query: 844  VVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIED 903
            +VFPQ+G RPHPNECSG DLDGD+YF+ WD +LIP +   PMDYT   P  +D  VT+E+
Sbjct: 851  LVFPQRGERPHPNECSGGDLDGDLYFITWDDKLIPEKVDTPMDYTATRPRIMDHVVTLEE 910

Query: 904  VQEYFVNYMVNDSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIP 963
            +Q++FV+YM+NDSLG I+ AH   AD+   KARS  C++LA L S+AVDF KTG PA +P
Sbjct: 911  IQKHFVDYMINDSLGAISTAHLIHADRSPLKARSPECLQLATLHSMAVDFAKTGAPAEMP 970

Query: 964  PELYVKEFPDFMDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVAR--QCYDSD 1023
              L  +E+PDFM++ +KP Y S  V+GKL+R+       +      +   A+    YD D
Sbjct: 971  RTLRPREYPDFMERWEKPMYISNGVLGKLYRSAMGHMEKSGDSGALSSSSAQPSPTYDPD 1030

Query: 1024 MEIEGYEDYVDDAIYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRD---- 1083
            +E+ G ++++  A  Y   Y+ KL  L++YY  + E EIL+GNI   +K    KRD    
Sbjct: 1031 LEVPGSDEFLQAAEEYYELYEEKLTTLMNYYRAELEDEILTGNI--RNKMLYLKRDNKRY 1090

Query: 1084 ---AEAINLAIRSLRKEARTWFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSY 1143
                + I  A+ +L +EAR W        D+  +         ASAWY VTYHP      
Sbjct: 1091 FEMKDRIVAAVDALHREARGWLLSSRKEEDASRM---------ASAWYRVTYHP------ 1120

Query: 1144 NEDMNRDHYLSFPWCVYDKLMQIKENNVRRKQR 1154
             +      + SFPW   D L+ IK ++  R++R
Sbjct: 1151 -DRRRGKRFWSFPWIACDNLLAIKASSQLRRRR 1120

BLAST of MS020748 vs. ExPASy Swiss-Prot
Match: Q8LHH9 (Probable RNA-dependent RNA polymerase SHL2 OS=Oryza sativa subsp. japonica OX=39947 GN=SHL2 PE=2 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 4.9e-168
Identity = 378/968 (39.05%), Postives = 543/968 (56.10%), Query Frame = 0

Query: 239  DDQWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFS- 298
            DD W+R+TD TPS  IG+     +   + R  P   +   Y +E       VD G G+  
Sbjct: 267  DDPWIRTTDITPSGAIGRCGVYRISF-SARFWPKMDRALDYMRER--RVAIVDCGGGWGP 326

Query: 299  ----CSADLVPMVDSRPDIF--------LPYKIIFKINALVQHGCISGPALDTSFYHLVD 358
                   D +   +   D+F        L + ++F +NALV  G I+   L   F+ L+ 
Sbjct: 327  RRGLTVRDELEFGEPMQDVFFCLQHAEGLKFPLLFMVNALVHKGIINQHQLTPEFFSLLG 386

Query: 359  PSRIKIEFVEHALDRLFHLKECEYKPYSWLTEQYKKYLRRPPNSPAI---SLDAGLVYVR 418
             S    E V  A  R F     +  P      + KK L R   +P +    +      VR
Sbjct: 387  RSE---ENVNVAALRDFW---GDKFPVFDACGRLKKALNRVARNPKLLCSKVGDDHAEVR 446

Query: 419  RVQITPCKVYFCGPEVNVSNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIK 478
            R+ ITP + Y   PEV  SNRVLRH+    D FLRV+F+DE    + +  L   T+  +K
Sbjct: 447  RLVITPTRAYCLPPEVERSNRVLRHYHEVADRFLRVTFMDEGMQVLNNNVLNSFTAPIVK 506

Query: 479  D-------RKTEIYKRILSVLKNGIVIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAAD 538
            D       +KT +YKR+  +L  G  + G+ + FLAFSS+QLRD S W FA         
Sbjct: 507  DLMSNFFQQKTTVYKRVRMLLTEGFHMCGRKYSFLAFSSNQLRDKSAWFFAEDRKTTVEA 566

Query: 539  IRAWMGDFRQIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGI 598
            IR WMG F   KN AK+AAR+GQ F S+  T+++   E +   D +V H E  Y+FSDGI
Sbjct: 567  IRKWMGRFTS-KNVAKHAARMGQCFSSTYATVTMRPDEVDESFD-DVVHNE--YIFSDGI 626

Query: 599  GKISSDFAKKVSAKCGLKAFTPSAFQIRYGGYKGVVAV--DPYSSKKLSLRKSMCKFESD 658
            GKI+ D A +V+ +  L    PSA+QIR+ G+KGV+AV        +L LR SM KFES+
Sbjct: 627  GKITPDLALEVAERLQLTDNPPSAYQIRFAGFKGVIAVWQGHGDGTRLFLRPSMRKFESN 686

Query: 659  NTKLDVLGYSKYQPCFLNRQLITLLSTLGVRDEVFEKKQHEAVKQLDAILTDPLKAQEAL 718
            +  L+V+ ++K+QP FLNRQ+I LLS+L V D +F + Q   +  L+ IL+D   A E L
Sbjct: 687  HLVLEVVSWTKFQPGFLNRQIIILLSSLNVPDSIFWQMQETMLSNLNNILSDRDVAFEVL 746

Query: 719  DLMAPGENTNVLKEMLKCGYKPNVEPYLSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGC 778
                  ++ N    ML  G++P  EP+L  ML A R+++L DL  K+R+F+P GR +MGC
Sbjct: 747  TTSC-ADDGNTAALMLSAGFEPRTEPHLKAMLLAIRSAQLQDLLEKARIFVPKGRWLMGC 806

Query: 779  LDETGSLEYGQVFVQVSGTKQSILSVTTSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIR 838
            LDE G LE GQ F++ +    +   V   +   ++ +    I G VV+AKNPCLHPGD+R
Sbjct: 807  LDELGVLEQGQCFIRATVPSLNSYFVKHGSRFSSTDKNTEVILGTVVIAKNPCLHPGDVR 866

Query: 839  VLKAVNVPQLYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPP--RQIPPM 898
            +L+AV+VP+L+H+VDC+VFPQKG RPH NE SGSDLDGD+YFV WD +LIPP  +   PM
Sbjct: 867  ILEAVDVPELHHLVDCLVFPQKGERPHANEASGSDLDGDLYFVTWDEKLIPPGKKSWNPM 926

Query: 899  DYTPAPPTQLDRDVTIEDVQEYFVNYMVNDSLGIIANAHTAFADKELFKARSSPCIELAK 958
            DY+P    QL R V+  D+ ++F+  M++++LG I NAH   AD   + A    CI LA+
Sbjct: 927  DYSPPEAKQLPRQVSQHDIIDFFLKNMISENLGRICNAHVVHADLSEYGAMDEKCIHLAE 986

Query: 959  LFSIAVDFPKTGVPAIIPPELYVKEFPDFMDKPDKPSYESKNVIGKLFRAVKDIAPTTSH 1018
            L + AVDFPKTG  AI+PP L  K +PDFM K D  SY+S+ ++G+L+R++++     S+
Sbjct: 987  LAATAVDFPKTGKLAIMPPHLKPKVYPDFMGKEDGQSYKSEKILGRLYRSIQE----ASN 1046

Query: 1019 ISLFTREVARQ---CYDSDMEIEGYEDYVDDAIYYKNNYDYKLGNLLDYYGIKSEAEILS 1078
              + ++EV       YD D+E+ G  D++  A   K +YD +L  LL  Y +++EAE+++
Sbjct: 1047 GDVVSQEVCTPNDLPYDIDLEVPGASDFLASAWQCKCSYDAQLSALLSQYRVRTEAELVT 1106

Query: 1079 GNIMTMSKSFTKKRD--AEAINLAIRSLRKEARTWF-----NERESGPDSDSVLGLDDLF 1138
            G+I  + K+ +KK+    + +  A  +LRKE ++ F     ++ E G D  ++L      
Sbjct: 1107 GHITFLVKNSSKKQGDIKDRLKTAYSALRKEFKSTFESIASDQCEIGDDEKNLL----YE 1166

Query: 1139 AKASAWYHVTYHPSYWGSYNEDMNRD-----HYLSFPWCVYDKLMQIK-----ENNVRRK 1160
             KASAWY VTYHP +       +  D       LSF W   D L +IK     +  V  +
Sbjct: 1167 MKASAWYQVTYHPKWVEKSRGILGPDGEEIPASLSFAWIPVDYLARIKLRCHGKVRVEGQ 1212

BLAST of MS020748 vs. ExPASy TrEMBL
Match: A0A6J1CYE0 (RNA-dependent RNA polymerase OS=Momordica charantia OX=3673 GN=LOC111015923 PE=3 SV=1)

HSP 1 Score: 2278.4 bits (5903), Expect = 0.0e+00
Identity = 1124/1172 (95.90%), Postives = 1134/1172 (96.76%), Query Frame = 0

Query: 1    MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60
            MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE
Sbjct: 1    MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60

Query: 61   LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120
            LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW
Sbjct: 61   LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120

Query: 121  EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLGSN 180
            EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVI      
Sbjct: 121  EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVI------ 180

Query: 181  SFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVPDD 240
                                    QLYGAP IYKKVESSSGSR+VFDDPLLNYFKEVPDD
Sbjct: 181  ------------------------QLYGAPWIYKKVESSSGSRQVFDDPLLNYFKEVPDD 240

Query: 241  QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSCSA 300
            QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFR VDGGNGFSCSA
Sbjct: 241  QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRLVDGGNGFSCSA 300

Query: 301  DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSRIKIEFVEHALDR 360
            DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDP RIKIEFVEHALDR
Sbjct: 301  DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPRRIKIEFVEHALDR 360

Query: 361  LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420
            LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV
Sbjct: 361  LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420

Query: 421  SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI 480
            SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI
Sbjct: 421  SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI 480

Query: 481  VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPAKYAARLGQSF 540
            VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDA DIRAWMGDFRQIKNPAKYAARLGQSF
Sbjct: 481  VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAEDIRAWMGDFRQIKNPAKYAARLGQSF 540

Query: 541  GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF 600
            GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF
Sbjct: 541  GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF 600

Query: 601  QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660
            QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST
Sbjct: 601  QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660

Query: 661  LGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGYKPNVEPY 720
            LGVRDEVFEKKQ EAV+QLDAILTDPLKAQEAL+LMAPGENTNVLKEMLKCGYKPNVEPY
Sbjct: 661  LGVRDEVFEKKQREAVEQLDAILTDPLKAQEALELMAPGENTNVLKEMLKCGYKPNVEPY 720

Query: 721  LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTKQSILSVT 780
            LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSG KQSILSVT
Sbjct: 721  LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGAKQSILSVT 780

Query: 781  TSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDCVVFPQKGSRPH 840
            TSAFN++SSEQRVAI+GNVVVAKNPCLHPGD+RVLKAVNVP+LYHMVDCVVFPQKGSRPH
Sbjct: 781  TSAFNISSSEQRVAIEGNVVVAKNPCLHPGDVRVLKAVNVPRLYHMVDCVVFPQKGSRPH 840

Query: 841  PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN 900
            PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN
Sbjct: 841  PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN 900

Query: 901  DSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF 960
            DSLGIIANAHTAFADKELFKARSSPCI LAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF
Sbjct: 901  DSLGIIANAHTAFADKELFKARSSPCINLAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF 960

Query: 961  MDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA 1020
            M+KPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA
Sbjct: 961  MEKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA 1020

Query: 1021 IYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART 1080
            IY+KNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART
Sbjct: 1021 IYHKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART 1080

Query: 1081 WFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL 1140
            WFNERESGPDSDSV GLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL
Sbjct: 1081 WFNERESGPDSDSVSGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL 1140

Query: 1141 MQIKENNVRRKQRASRLAALDSRFRNALNLGH 1173
            MQIKENNVRRKQRASRLAALDSRFRNALNLGH
Sbjct: 1141 MQIKENNVRRKQRASRLAALDSRFRNALNLGH 1142

BLAST of MS020748 vs. ExPASy TrEMBL
Match: A0A6J1D0H1 (RNA-dependent RNA polymerase OS=Momordica charantia OX=3673 GN=LOC111015923 PE=3 SV=1)

HSP 1 Score: 2191.8 bits (5678), Expect = 0.0e+00
Identity = 1091/1172 (93.09%), Postives = 1101/1172 (93.94%), Query Frame = 0

Query: 1    MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60
            MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE
Sbjct: 1    MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60

Query: 61   LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120
            LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW
Sbjct: 61   LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120

Query: 121  EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLGSN 180
            EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVI      
Sbjct: 121  EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVI------ 180

Query: 181  SFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVPDD 240
                                    QLYGAP IYKKVESSSGSR+VFDDPLLNYFKEVPDD
Sbjct: 181  ------------------------QLYGAPWIYKKVESSSGSRQVFDDPLLNYFKEVPDD 240

Query: 241  QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSCSA 300
            QW                                 NFAYYKETEDEFR VDGGNGFSCSA
Sbjct: 241  QW---------------------------------NFAYYKETEDEFRLVDGGNGFSCSA 300

Query: 301  DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSRIKIEFVEHALDR 360
            DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDP RIKIEFVEHALDR
Sbjct: 301  DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPRRIKIEFVEHALDR 360

Query: 361  LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420
            LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV
Sbjct: 361  LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420

Query: 421  SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI 480
            SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI
Sbjct: 421  SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI 480

Query: 481  VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPAKYAARLGQSF 540
            VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDA DIRAWMGDFRQIKNPAKYAARLGQSF
Sbjct: 481  VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAEDIRAWMGDFRQIKNPAKYAARLGQSF 540

Query: 541  GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF 600
            GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF
Sbjct: 541  GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF 600

Query: 601  QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660
            QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST
Sbjct: 601  QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660

Query: 661  LGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGYKPNVEPY 720
            LGVRDEVFEKKQ EAV+QLDAILTDPLKAQEAL+LMAPGENTNVLKEMLKCGYKPNVEPY
Sbjct: 661  LGVRDEVFEKKQREAVEQLDAILTDPLKAQEALELMAPGENTNVLKEMLKCGYKPNVEPY 720

Query: 721  LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTKQSILSVT 780
            LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSG KQSILSVT
Sbjct: 721  LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGAKQSILSVT 780

Query: 781  TSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDCVVFPQKGSRPH 840
            TSAFN++SSEQRVAI+GNVVVAKNPCLHPGD+RVLKAVNVP+LYHMVDCVVFPQKGSRPH
Sbjct: 781  TSAFNISSSEQRVAIEGNVVVAKNPCLHPGDVRVLKAVNVPRLYHMVDCVVFPQKGSRPH 840

Query: 841  PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN 900
            PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN
Sbjct: 841  PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN 900

Query: 901  DSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF 960
            DSLGIIANAHTAFADKELFKARSSPCI LAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF
Sbjct: 901  DSLGIIANAHTAFADKELFKARSSPCINLAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF 960

Query: 961  MDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA 1020
            M+KPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA
Sbjct: 961  MEKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA 1020

Query: 1021 IYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART 1080
            IY+KNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART
Sbjct: 1021 IYHKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART 1080

Query: 1081 WFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL 1140
            WFNERESGPDSDSV GLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL
Sbjct: 1081 WFNERESGPDSDSVSGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL 1109

Query: 1141 MQIKENNVRRKQRASRLAALDSRFRNALNLGH 1173
            MQIKENNVRRKQRASRLAALDSRFRNALNLGH
Sbjct: 1141 MQIKENNVRRKQRASRLAALDSRFRNALNLGH 1109

BLAST of MS020748 vs. ExPASy TrEMBL
Match: A0A6J1FXH5 (RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111447985 PE=3 SV=1)

HSP 1 Score: 1892.5 bits (4901), Expect = 0.0e+00
Identity = 935/1170 (79.91%), Postives = 1031/1170 (88.12%), Query Frame = 0

Query: 1    MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60
            MGKTVHISGFP+  TAD+V+S+LE RTG+GTVYA+K+R PK+GGSR+YA VQF G+ +AE
Sbjct: 1    MGKTVHISGFPSHATADSVKSFLELRTGLGTVYAIKVRPPKRGGSRVYAIVQFIGAAQAE 60

Query: 61   LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120
            LIISLAN   QRLWYG+SYLKAR ADVDI+PKPRTY+HTLEDLTLCFGCQVSSEKF VLW
Sbjct: 61   LIISLAN---QRLWYGSSYLKARAADVDIVPKPRTYVHTLEDLTLCFGCQVSSEKFHVLW 120

Query: 121  EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLGSN 180
            EG   LVTFG  MRKMNF L HNFVEYRF+LSYENIW+I+LH  +HQSMK L+I      
Sbjct: 121  EGDVELVTFGVEMRKMNFHLIHNFVEYRFQLSYENIWQIQLHRLQHQSMKCLLI------ 180

Query: 181  SFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVPDD 240
                                    QLYGAPRIYKKV  SSG  ++FDDPL N+FKEVPDD
Sbjct: 181  ------------------------QLYGAPRIYKKVAPSSG--QIFDDPLFNFFKEVPDD 240

Query: 241  QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSCSA 300
            QWVR+ DFTPS+ +GQSSSLCLKL N R+LPNF ++FAYY+E EDEF+ VDGGN FSCS 
Sbjct: 241  QWVRTADFTPSNSVGQSSSLCLKLPNGRQLPNFRRHFAYYEEFEDEFKLVDGGNSFSCSD 300

Query: 301  DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSRIKIEFVEHALDR 360
            DLVP+VDSRP++ LPY+IIFK+N LVQ+GCI+GP+LDT FY LVDPSRI++E+VE+AL++
Sbjct: 301  DLVPVVDSRPNVLLPYEIIFKVNVLVQNGCIAGPSLDTGFYRLVDPSRIRVEYVENALEK 360

Query: 361  LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420
            LFHLKEC Y+P S+LTEQYKKY + P NSPAI+LD GLVYVRRVQITPCKVYFCGPEVNV
Sbjct: 361  LFHLKECCYEPSSFLTEQYKKYAKHPSNSPAIALDDGLVYVRRVQITPCKVYFCGPEVNV 420

Query: 421  SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI 480
            SNRVLRHFSRD+DNFLRVSFVDEEWDKMRS DLLPR +S+I++ KT+IY+RILSVLKNGI
Sbjct: 421  SNRVLRHFSRDLDNFLRVSFVDEEWDKMRSTDLLPRMASSIENGKTDIYRRILSVLKNGI 480

Query: 481  VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPAKYAARLGQSF 540
             IG K FKFLAFSSSQLR+NSLWMFA+RPGLDAA IR WMGDFR IKNPAKYAARLGQSF
Sbjct: 481  AIGDKKFKFLAFSSSQLRENSLWMFASRPGLDAAVIREWMGDFRHIKNPAKYAARLGQSF 540

Query: 541  GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF 600
            GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAK+V++KC L AFTPSAF
Sbjct: 541  GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKRVASKCVL-AFTPSAF 600

Query: 601  QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660
            QIRYGGYKGVVAVDP SS KLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST
Sbjct: 601  QIRYGGYKGVVAVDPRSSVKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660

Query: 661  LGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGYKPNVEPY 720
            LGV+DEVFEKKQ EA++QLDAILTDPLKAQEAL+LM+PGENTN+LKEMLKCGYKP+VEPY
Sbjct: 661  LGVKDEVFEKKQSEALEQLDAILTDPLKAQEALELMSPGENTNILKEMLKCGYKPDVEPY 720

Query: 721  LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTKQSILSVT 780
            LSMMLQ FR SKLL+LRTKSR+FIPNGRAMMGCLDETG+LEYGQVFVQ+S  +   +S  
Sbjct: 721  LSMMLQTFRESKLLELRTKSRIFIPNGRAMMGCLDETGTLEYGQVFVQISSVRHRHIS-D 780

Query: 781  TSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDCVVFPQKGSRPH 840
            TSAF+M+ SE  + I GNV VAKNPCLHPGD+RVL AVNVP LYHMVDCVVFPQKGSRPH
Sbjct: 781  TSAFDMSGSEDHLVIVGNVTVAKNPCLHPGDVRVLNAVNVPLLYHMVDCVVFPQKGSRPH 840

Query: 841  PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN 900
            PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPP QLDRDVTIEDVQEYFVNYMVN
Sbjct: 841  PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPIQLDRDVTIEDVQEYFVNYMVN 900

Query: 901  DSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF 960
            DSLGIIANAHTAFADKE FKARS+PCIELAKLFSIAVDFPKTGVPAIIP  L VKEFPDF
Sbjct: 901  DSLGIIANAHTAFADKEPFKARSAPCIELAKLFSIAVDFPKTGVPAIIPSHLNVKEFPDF 960

Query: 961  MDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA 1020
            M+KPD+PSYESKNVIGKLFRAVK IAP  S+I  FTR+VA +CYDSDME EG+EDYV+DA
Sbjct: 961  MEKPDRPSYESKNVIGKLFRAVKGIAPNISYIRSFTRDVAMRCYDSDMETEGFEDYVEDA 1020

Query: 1021 IYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART 1080
            IY+K  YDYKLGNLLDYYGIKSE+EI SGNIM MSKSFT++RDAEAINLA RSLRKEART
Sbjct: 1021 IYHKTIYDYKLGNLLDYYGIKSESEIFSGNIMRMSKSFTRRRDAEAINLAARSLRKEART 1080

Query: 1081 WFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL 1140
            WFN +ESGPDS    G DDLFAKASAWYHVTYH SYWGSYN+ M RDHYLSFPWCVY KL
Sbjct: 1081 WFNTKESGPDS----GSDDLFAKASAWYHVTYHHSYWGSYNDGMKRDHYLSFPWCVYHKL 1128

Query: 1141 MQIKENNVRRKQRASRLAALDSRFRNALNL 1171
            MQ+KENN+RR+++ASRLA LD RF   LNL
Sbjct: 1141 MQVKENNLRRREKASRLATLD-RFSRVLNL 1128

BLAST of MS020748 vs. ExPASy TrEMBL
Match: A0A6J1FSA2 (RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111447985 PE=3 SV=1)

HSP 1 Score: 1892.5 bits (4901), Expect = 0.0e+00
Identity = 935/1170 (79.91%), Postives = 1031/1170 (88.12%), Query Frame = 0

Query: 1    MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60
            MGKTVHISGFP+  TAD+V+S+LE RTG+GTVYA+K+R PK+GGSR+YA VQF G+ +AE
Sbjct: 15   MGKTVHISGFPSHATADSVKSFLELRTGLGTVYAIKVRPPKRGGSRVYAIVQFIGAAQAE 74

Query: 61   LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120
            LIISLAN   QRLWYG+SYLKAR ADVDI+PKPRTY+HTLEDLTLCFGCQVSSEKF VLW
Sbjct: 75   LIISLAN---QRLWYGSSYLKARAADVDIVPKPRTYVHTLEDLTLCFGCQVSSEKFHVLW 134

Query: 121  EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLGSN 180
            EG   LVTFG  MRKMNF L HNFVEYRF+LSYENIW+I+LH  +HQSMK L+I      
Sbjct: 135  EGDVELVTFGVEMRKMNFHLIHNFVEYRFQLSYENIWQIQLHRLQHQSMKCLLI------ 194

Query: 181  SFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVPDD 240
                                    QLYGAPRIYKKV  SSG  ++FDDPL N+FKEVPDD
Sbjct: 195  ------------------------QLYGAPRIYKKVAPSSG--QIFDDPLFNFFKEVPDD 254

Query: 241  QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSCSA 300
            QWVR+ DFTPS+ +GQSSSLCLKL N R+LPNF ++FAYY+E EDEF+ VDGGN FSCS 
Sbjct: 255  QWVRTADFTPSNSVGQSSSLCLKLPNGRQLPNFRRHFAYYEEFEDEFKLVDGGNSFSCSD 314

Query: 301  DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSRIKIEFVEHALDR 360
            DLVP+VDSRP++ LPY+IIFK+N LVQ+GCI+GP+LDT FY LVDPSRI++E+VE+AL++
Sbjct: 315  DLVPVVDSRPNVLLPYEIIFKVNVLVQNGCIAGPSLDTGFYRLVDPSRIRVEYVENALEK 374

Query: 361  LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420
            LFHLKEC Y+P S+LTEQYKKY + P NSPAI+LD GLVYVRRVQITPCKVYFCGPEVNV
Sbjct: 375  LFHLKECCYEPSSFLTEQYKKYAKHPSNSPAIALDDGLVYVRRVQITPCKVYFCGPEVNV 434

Query: 421  SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI 480
            SNRVLRHFSRD+DNFLRVSFVDEEWDKMRS DLLPR +S+I++ KT+IY+RILSVLKNGI
Sbjct: 435  SNRVLRHFSRDLDNFLRVSFVDEEWDKMRSTDLLPRMASSIENGKTDIYRRILSVLKNGI 494

Query: 481  VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPAKYAARLGQSF 540
             IG K FKFLAFSSSQLR+NSLWMFA+RPGLDAA IR WMGDFR IKNPAKYAARLGQSF
Sbjct: 495  AIGDKKFKFLAFSSSQLRENSLWMFASRPGLDAAVIREWMGDFRHIKNPAKYAARLGQSF 554

Query: 541  GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF 600
            GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAK+V++KC L AFTPSAF
Sbjct: 555  GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKRVASKCVL-AFTPSAF 614

Query: 601  QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660
            QIRYGGYKGVVAVDP SS KLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST
Sbjct: 615  QIRYGGYKGVVAVDPRSSVKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 674

Query: 661  LGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGYKPNVEPY 720
            LGV+DEVFEKKQ EA++QLDAILTDPLKAQEAL+LM+PGENTN+LKEMLKCGYKP+VEPY
Sbjct: 675  LGVKDEVFEKKQSEALEQLDAILTDPLKAQEALELMSPGENTNILKEMLKCGYKPDVEPY 734

Query: 721  LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTKQSILSVT 780
            LSMMLQ FR SKLL+LRTKSR+FIPNGRAMMGCLDETG+LEYGQVFVQ+S  +   +S  
Sbjct: 735  LSMMLQTFRESKLLELRTKSRIFIPNGRAMMGCLDETGTLEYGQVFVQISSVRHRHIS-D 794

Query: 781  TSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDCVVFPQKGSRPH 840
            TSAF+M+ SE  + I GNV VAKNPCLHPGD+RVL AVNVP LYHMVDCVVFPQKGSRPH
Sbjct: 795  TSAFDMSGSEDHLVIVGNVTVAKNPCLHPGDVRVLNAVNVPLLYHMVDCVVFPQKGSRPH 854

Query: 841  PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN 900
            PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPP QLDRDVTIEDVQEYFVNYMVN
Sbjct: 855  PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPIQLDRDVTIEDVQEYFVNYMVN 914

Query: 901  DSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF 960
            DSLGIIANAHTAFADKE FKARS+PCIELAKLFSIAVDFPKTGVPAIIP  L VKEFPDF
Sbjct: 915  DSLGIIANAHTAFADKEPFKARSAPCIELAKLFSIAVDFPKTGVPAIIPSHLNVKEFPDF 974

Query: 961  MDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA 1020
            M+KPD+PSYESKNVIGKLFRAVK IAP  S+I  FTR+VA +CYDSDME EG+EDYV+DA
Sbjct: 975  MEKPDRPSYESKNVIGKLFRAVKGIAPNISYIRSFTRDVAMRCYDSDMETEGFEDYVEDA 1034

Query: 1021 IYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART 1080
            IY+K  YDYKLGNLLDYYGIKSE+EI SGNIM MSKSFT++RDAEAINLA RSLRKEART
Sbjct: 1035 IYHKTIYDYKLGNLLDYYGIKSESEIFSGNIMRMSKSFTRRRDAEAINLAARSLRKEART 1094

Query: 1081 WFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL 1140
            WFN +ESGPDS    G DDLFAKASAWYHVTYH SYWGSYN+ M RDHYLSFPWCVY KL
Sbjct: 1095 WFNTKESGPDS----GSDDLFAKASAWYHVTYHHSYWGSYNDGMKRDHYLSFPWCVYHKL 1142

Query: 1141 MQIKENNVRRKQRASRLAALDSRFRNALNL 1171
            MQ+KENN+RR+++ASRLA LD RF   LNL
Sbjct: 1155 MQVKENNLRRREKASRLATLD-RFSRVLNL 1142

BLAST of MS020748 vs. ExPASy TrEMBL
Match: A0A6J1K2P9 (RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111490498 PE=3 SV=1)

HSP 1 Score: 1880.1 bits (4869), Expect = 0.0e+00
Identity = 933/1170 (79.74%), Postives = 1028/1170 (87.86%), Query Frame = 0

Query: 1    MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60
            MGKTVHISGFP+  TAD+V+S+LE RTG+GTVYA+K+R PK+GGSR+YA VQFTG+ +AE
Sbjct: 1    MGKTVHISGFPSHATADSVKSFLELRTGLGTVYAIKVRPPKRGGSRVYAIVQFTGAAQAE 60

Query: 61   LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120
            LIISLAN   QRLWYG+SYLKAR ADVDI+PKPRTY+HTLE+LTLCFGCQVSSEKF VLW
Sbjct: 61   LIISLAN---QRLWYGSSYLKARAADVDIVPKPRTYVHTLENLTLCFGCQVSSEKFHVLW 120

Query: 121  EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLGSN 180
            EG   LVTFG  MRKMNF L HNFVEYRF+LSYENIW+I+LH  +HQSMK L+I      
Sbjct: 121  EGDVELVTFGVEMRKMNFHLIHNFVEYRFQLSYENIWQIQLHRLQHQSMKCLLI------ 180

Query: 181  SFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVPDD 240
                                    QLYGAPRIYKKV  SSG  ++FDDPL N+FKEVPDD
Sbjct: 181  ------------------------QLYGAPRIYKKVAPSSG--QIFDDPLFNFFKEVPDD 240

Query: 241  QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSCSA 300
            Q VR+ DFTPS  IGQSSSLCLKL N R+LPNF ++FAYY+E EDEF+ VDGGNGFSCS 
Sbjct: 241  QCVRTADFTPSYSIGQSSSLCLKLPNGRQLPNFRRHFAYYEEFEDEFKLVDGGNGFSCSD 300

Query: 301  DLVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSRIKIEFVEHALDR 360
            DLVP+VDS P++ LPY+IIFK+N LVQ+GCI GP+LDT FY LVDPSRI++E+VE+AL++
Sbjct: 301  DLVPVVDSHPNVLLPYEIIFKVNVLVQNGCIPGPSLDTGFYRLVDPSRIRVEYVENALEK 360

Query: 361  LFHLKECEYKPYSWLTEQYKKYLRRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNV 420
            LFHLKEC Y+P S+LTEQYKKY + P NSPAI+LDAGLVYVRRVQITPCKVYFCGPEVNV
Sbjct: 361  LFHLKECCYEPSSFLTEQYKKYAKHPSNSPAIALDAGLVYVRRVQITPCKVYFCGPEVNV 420

Query: 421  SNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLKNGI 480
            SNRVLRHFSRD+DNFLRVSFVDEEWDKMRS DLLPR +S+I++ KT+IY+RILSVLKNGI
Sbjct: 421  SNRVLRHFSRDLDNFLRVSFVDEEWDKMRSTDLLPRMASSIENGKTDIYRRILSVLKNGI 480

Query: 481  VIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPAKYAARLGQSF 540
             IG K FKFLAFSSSQLR+NSLWMFA+RPGLDAA IR WMGDF+ IKNPAKYAARLGQSF
Sbjct: 481  AIGDKKFKFLAFSSSQLRENSLWMFASRPGLDAAVIREWMGDFQHIKNPAKYAARLGQSF 540

Query: 541  GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTPSAF 600
            GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAK+V++KC L AFTPSAF
Sbjct: 541  GSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKRVASKCVL-AFTPSAF 600

Query: 601  QIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660
            QIRYGGYKGVVAVDP SS KLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST
Sbjct: 601  QIRYGGYKGVVAVDPRSSVKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLST 660

Query: 661  LGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGYKPNVEPY 720
            LGV+DEVFEKKQ EA++QLDAILTDPLKAQEAL+LM+PGENTN+LKEMLKCGYKP+VEPY
Sbjct: 661  LGVKDEVFEKKQSEALEQLDAILTDPLKAQEALELMSPGENTNILKEMLKCGYKPDVEPY 720

Query: 721  LSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTKQSILSVT 780
            LSMMLQ FR SKLL+LRTKSR+FIPNGRAMMGCLDETG+LEYGQVFVQ+S  +   +S  
Sbjct: 721  LSMMLQTFRESKLLELRTKSRIFIPNGRAMMGCLDETGTLEYGQVFVQISNARHRHIS-D 780

Query: 781  TSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDCVVFPQKGSRPH 840
            TSAF+M+ SE  + I GNV VAKNPCLHPGD+RVL AVNVP LYHMVDCVVFPQKGSRPH
Sbjct: 781  TSAFDMSGSEDHLVIVGNVTVAKNPCLHPGDVRVLNAVNVPLLYHMVDCVVFPQKGSRPH 840

Query: 841  PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVN 900
            PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPP QLDRDVTIEDVQEYFVNYMVN
Sbjct: 841  PNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPIQLDRDVTIEDVQEYFVNYMVN 900

Query: 901  DSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIPPELYVKEFPDF 960
            DSLGIIANAHTAFADKE FKARS+PCIELAKLFSIAVDFPKTGVPAIIP  L VKEFPDF
Sbjct: 901  DSLGIIANAHTAFADKEPFKARSAPCIELAKLFSIAVDFPKTGVPAIIPSHLNVKEFPDF 960

Query: 961  MDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYVDDA 1020
            M+KPD+ SYESKNVIGKLFRAVK IAP  S+I  FTR+VA +CYDSDME EG+EDYV+DA
Sbjct: 961  MEKPDRHSYESKNVIGKLFRAVKGIAPNISYIRSFTRDVAMRCYDSDMEAEGFEDYVEDA 1020

Query: 1021 IYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKEART 1080
            IY+K  YDYKLGNLLDYYGIKSE+EI SGNIM MSKSFT++RDAEAINLA RSLRKEART
Sbjct: 1021 IYHKTIYDYKLGNLLDYYGIKSESEIFSGNIMRMSKSFTRRRDAEAINLAARSLRKEART 1080

Query: 1081 WFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVYDKL 1140
            WFN +ESG DS    G DDLFAKASAWYHVTYH SYWGSYN+ M RDHYLSFPWCVY KL
Sbjct: 1081 WFNTKESGLDS----GSDDLFAKASAWYHVTYHHSYWGSYNDGMKRDHYLSFPWCVYHKL 1128

Query: 1141 MQIKENNVRRKQRASRLAALDSRFRNALNL 1171
            MQ+KENN+R+++RASRLA LD RF   LNL
Sbjct: 1141 MQVKENNLRKRERASRLATLD-RFSRVLNL 1128

BLAST of MS020748 vs. TAIR 10
Match: AT1G14790.1 (RNA-dependent RNA polymerase 1 )

HSP 1 Score: 1407.5 bits (3642), Expect = 0.0e+00
Identity = 711/1168 (60.87%), Postives = 871/1168 (74.57%), Query Frame = 0

Query: 1    MGKTVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGSRLYAFVQFTGSREAE 60
            MGKT+ + GFP GV+A+ V+ +LE  TG GTVYA+K+R+PKKGG R+YA VQFT  R   
Sbjct: 1    MGKTIQVFGFPNGVSAEEVKKFLERLTGSGTVYAIKVRQPKKGGPRVYAIVQFTSERHTR 60

Query: 61   LIISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEKFCVLW 120
            LII+ A    +RL+YG SYLKA E + DI+PKPR  +HT+  L + FGCQVS++KF  LW
Sbjct: 61   LIITAA---AERLYYGRSYLKAFEVEQDIVPKPRASLHTISGLKMFFGCQVSTKKFLTLW 120

Query: 121  EGRDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLGSN 180
              +DV V+FG GMRK++F  S    +YR ELSYENIW+I+LH  + +S K+LVIQV+   
Sbjct: 121  SAQDVCVSFGIGMRKLHFSFSWYQKDYRLELSYENIWQIDLHSPQGRSSKFLVIQVI--- 180

Query: 181  SFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVPDD 240
                                       GAP+I++K +       +F   +++++ +  D+
Sbjct: 181  ---------------------------GAPKIFEKEDQP--INLLFG--IMDFYSDGSDE 240

Query: 241  QWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSCSA 300
            QW+R+TDFT SSCIGQS++ CL+L     +P+F +NFA Y E       ++ G+ +S +A
Sbjct: 241  QWIRTTDFTSSSCIGQSTAFCLELPVHLNVPDFRENFANYAEHRASSFLIESGSSYSSNA 300

Query: 301  D-LVPMVDSRPDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSRIKIEFVEHALD 360
            + LVP+VD  P   LP++I+FK+N LVQ+ C+SGPALD  FY L++  +     ++H L+
Sbjct: 301  NTLVPVVDPPPGFSLPFEILFKLNTLVQNACLSGPALDLDFYRLLNQKKYDRALIDHCLE 360

Query: 361  RLFHLKECEYKPYSWLTEQYKKYLRRP--PNSPAISLDAGLVYVRRVQITPCKVYFCGPE 420
            +LFHL EC Y+P  WL ++YKK++ +   P SP ISLD GLVY+ RVQ+TP +VYF GPE
Sbjct: 361  KLFHLGECCYEPAHWLRDEYKKWISKGKLPLSPTISLDDGLVYMYRVQVTPARVYFSGPE 420

Query: 421  VNVSNRVLRHFSRDIDNFLRVSFVDEEWDKMRSIDLLPRTSSTIKDRKTEIYKRILSVLK 480
            VNVSNRVLRH+S+ I+NFLRVSFVDE+ +K+RS+DL PR+S+    R+T++Y RI SVL+
Sbjct: 421  VNVSNRVLRHYSKYINNFLRVSFVDEDLEKVRSMDLSPRSST---QRRTKLYDRIYSVLR 480

Query: 481  NGIVIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPAKYAARLG 540
            +GIVIG K F+FLAFSSSQLR+NS WMFA    + AA IRAWMGDF  I+N AKYAARLG
Sbjct: 481  DGIVIGDKKFEFLAFSSSQLRENSAWMFAPIDRITAAHIRAWMGDFDHIRNVAKYAARLG 540

Query: 541  QSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKAFTP 600
            QSF SS ETL+V   E E+IPD+E+     +YVFSDGIGKIS++FA+KV+ KCGL  F+P
Sbjct: 541  QSFSSSRETLNVRSDEIEVIPDVEIISLGTRYVFSDGIGKISAEFARKVARKCGLTEFSP 600

Query: 601  SAFQIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITL 660
            SAFQIRYGGYKGVVAVDP SSKKLSLRKSM KFES+NTKLDVL +SKYQPC++NRQLITL
Sbjct: 601  SAFQIRYGGYKGVVAVDPNSSKKLSLRKSMSKFESENTKLDVLAWSKYQPCYMNRQLITL 660

Query: 661  LSTLGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGYKPNV 720
            LSTLGV D VFEKKQ E V +LDAILT PL+A EAL LMAPGENTN+LK ++ CGYKP+ 
Sbjct: 661  LSTLGVTDSVFEKKQREVVDRLDAILTHPLEAHEALGLMAPGENTNILKALILCGYKPDA 720

Query: 721  EPYLSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTKQSIL 780
            EP+LSMMLQ FRASKLL+LRTK+R+FI  GR+MMGCLDET +LEYGQV VQ S   +   
Sbjct: 721  EPFLSMMLQNFRASKLLELRTKTRIFISGGRSMMGCLDETRTLEYGQVVVQYSDPMR--- 780

Query: 781  SVTTSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDCVVFPQKGS 840
                         +R  I G VVVAKNPCLHPGD+RVL+AVNVP L HMVDCVVFPQKG 
Sbjct: 781  -----------PGRRFIITGPVVVAKNPCLHPGDVRVLQAVNVPALNHMVDCVVFPQKGL 840

Query: 841  RPHPNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNY 900
            RPHPNECSGSDLDGDIYFVCWD EL+PPR   PMDYTP P   LD DVTIE+V+EYF NY
Sbjct: 841  RPHPNECSGSDLDGDIYFVCWDQELVPPRTSEPMDYTPEPTQILDHDVTIEEVEEYFANY 900

Query: 901  MVNDSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIPPELYVKEF 960
            +VNDSLGIIANAHTAFADKE  KA S PCIELAK FS AVDFPKTGV A+IP  LYVKE+
Sbjct: 901  IVNDSLGIIANAHTAFADKEPLKAFSDPCIELAKKFSTAVDFPKTGVAAVIPQHLYVKEY 960

Query: 961  PDFMDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDMEIEGYEDYV 1020
            PDFM+KPDKP+YESKNVIGKLFR VK+ AP    I  FT +VA + YD DME++G+E+YV
Sbjct: 961  PDFMEKPDKPTYESKNVIGKLFREVKERAPPLISIKSFTLDVASKSYDKDMEVDGFEEYV 1020

Query: 1021 DDAIYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRDAEAINLAIRSLRKE 1080
            D+A Y K NYD+KLGNL+DYYGIK+EAEILSG IM MSKSFTK+RDAE+I  A+R+LRKE
Sbjct: 1021 DEAFYQKANYDFKLGNLMDYYGIKTEAEILSGGIMRMSKSFTKRRDAESIGRAVRALRKE 1080

Query: 1081 ARTWFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYNEDMNRDHYLSFPWCVY 1140
              + FN  E   +           AKASAWYHVTYH SYWG YNE +NRDH+LSF WCVY
Sbjct: 1081 TLSLFNASEEEENES---------AKASAWYHVTYHSSYWGLYNEGLNRDHFLSFAWCVY 1105

Query: 1141 DKLMQIKENNVRRKQRASRLAALDSRFR 1166
            DKL++IK+ N+ R+QR   L  LD   R
Sbjct: 1141 DKLVRIKKTNLGRRQRQETLERLDHVLR 1105

BLAST of MS020748 vs. TAIR 10
Match: AT4G11130.1 (RNA-dependent RNA polymerase 2 )

HSP 1 Score: 758.4 bits (1957), Expect = 8.2e-219
Identity = 466/1181 (39.46%), Postives = 678/1181 (57.41%), Query Frame = 0

Query: 4    TVHISGFPAGVTADAVRSYLEGRTGVGTVYALKIREPKKGGS-RLYAFVQFTGSREAELI 63
            TV IS  P  + AD +  +LE   G  TV+AL+I   +     R +A VQFT        
Sbjct: 11   TVKISNVPQTIVADELLRFLELHLGEDTVFALEIPTTRDNWKPRDFARVQFTTLEVKSRA 70

Query: 64   ISLANQRIQRLWYGTSYLKAREADVDIIPKPRTYMHTLEDLTLCFGCQVSSEK-FCVL-- 123
              L++Q   +L + T  L+  EA  DIIP+P      L+D+ L  G   S EK FC L  
Sbjct: 71   QLLSSQ--SKLLFKTHNLRLSEAYDDIIPRPVDPRKRLDDIVLTVGFPESDEKRFCALEK 130

Query: 124  WEG-RDVLVTFGFGMRKMNFRLSHNFVEYRFELSYENIWRIELHHSRHQSMKYLVIQVLG 183
            W+G R  ++T     R++ F +  +   Y+ E+ +E+I            ++ L   V G
Sbjct: 131  WDGVRCWILT---EKRRVEFWVWESGDCYKIEVRFEDI------------IETLSCCVNG 190

Query: 184  SNSFPKLSHAILMLKIHQIIVMFFFSQLYGAPRIYKKVESSSGSRRVFDDPLLNYFKEVP 243
              S  ++   +L LK             YG P+++K+V     ++  F      + KE  
Sbjct: 191  DAS--EIDAFLLKLK-------------YG-PKVFKRVTVHIATK--FKSDRYRFCKEDF 250

Query: 244  DDQWVRSTDFTPSSCIGQSSSLCLKLHNCRELPNFGQNFAYYKETEDEFRFVDGGNGFSC 303
            D  W+R+TDF+ S  IG S+  CL++HN   + +      YY+E      +VD G  F+ 
Sbjct: 251  DFMWIRTTDFSGSKSIGTSTCFCLEVHNGSTMLDIFSGLPYYREDTLSLTYVD-GKTFAS 310

Query: 304  SADLVPMVDSR-PDIFLPYKIIFKINALVQHGCISGPALDTSFYHLVDPSR-IKIEFVEH 363
            +A +VP++++    +  PY+I+F++NALV    IS      S   L+   R + +E    
Sbjct: 311  AAQIVPLLNAAILGLEFPYEILFQLNALVHAQKIS--LFAASDMELIKILRGMSLETALV 370

Query: 364  ALDRLFHLKECEYKPYSWLTEQYKKYLRRPPNSPAIS----LDAGLVYVRRVQITPCKVY 423
             L +L       Y P  ++  Q +  +++  +SPA +     +  ++  +R  +TP K+Y
Sbjct: 371  ILKKLHQQSSICYDPVFFVKTQMQSVVKKMKHSPASAYKRLTEQNIMSCQRAYVTPSKIY 430

Query: 424  FCGPEVNVSNRVLRHFSRDIDNFLRVSFVDEEWDKM--RSIDLLPRTSSTIKDRKTEIYK 483
              GPE+  +N V+++F+  + +F+RV+FV+E+W K+   ++ +  +    +K  +T IY 
Sbjct: 431  LLGPELETANYVVKNFAEHVSDFMRVTFVEEDWSKLPANALSVNSKEGYFVKPSRTNIYN 490

Query: 484  RILSVLKNGIVIGGKMFKFLAFSSSQLRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPA 543
            R+LS+L  GI +G K F+FLAFS+SQLR NS+WMFA+   + A DIR WMG FR+I++ +
Sbjct: 491  RVLSILGEGITVGPKRFEFLAFSASQLRGNSVWMFASNEKVKAEDIREWMGCFRKIRSIS 550

Query: 544  KYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKC 603
            K AAR+GQ F +S +TL V   + E IPDIEV      Y FSDGIGKIS  FAK+V+ KC
Sbjct: 551  KCAARMGQLFSASRQTLIVRAQDVEQIPDIEVTTDGADYCFSDGIGKISLAFAKQVAQKC 610

Query: 604  GLKAFTPSAFQIRYGGYKGVVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFL 663
            GL +  PSAFQIRYGGYKGV+AVD  S +KLSLR SM KF+S+N  L+V  +++  PCFL
Sbjct: 611  GL-SHVPSAFQIRYGGYKGVIAVDRSSFRKLSLRDSMLKFDSNNRMLNVTRWTESMPCFL 670

Query: 664  NRQLITLLSTLGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLK 723
            NR++I LLSTLG+ D +FE  Q   +  L  +L D   A   L  ++   + N+L +ML 
Sbjct: 671  NREIICLLSTLGIEDAMFEAMQAVHLSMLGNMLEDRDAALNVLQKLSGENSKNLLVKMLL 730

Query: 724  CGYKPNVEPYLSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVS 783
             GY P+ EPYLSMML+    S+L +L+++ R+ +P GR ++GC+DE G LEYGQV+V+V+
Sbjct: 731  QGYAPSSEPYLSMMLRVHHESQLSELKSRCRILVPKGRILIGCMDEMGILEYGQVYVRVT 790

Query: 784  GTKQSILSVTTSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYH--MVD 843
             TK  + S   S F     E  V I G VVV KNPCLHPGDIRVL A+          +D
Sbjct: 791  LTKAELKSRDQSYFRKIDEETSVVI-GKVVVTKNPCLHPGDIRVLDAIYEVHFEEKGYLD 850

Query: 844  CVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPAPPTQLDRDVTIE 903
            C++FPQKG RPHPNECSG DLDGD +FV WD ++IP    PPMDY  + P  +D DVT+E
Sbjct: 851  CIIFPQKGERPHPNECSGGDLDGDQFFVSWDEKIIPSEMDPPMDYAGSRPRLMDHDVTLE 910

Query: 904  DVQEYFVNYMVNDSLGIIANAHTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAII 963
            ++ ++FV+YM++D+LG+I+ AH   AD++  KARS  C+ELA L S AVDF KTG PA +
Sbjct: 911  EIHKFFVDYMISDTLGVISTAHLVHADRDPEKARSQKCLELANLHSRAVDFAKTGAPAEM 970

Query: 964  PPELYVKEFPDFMDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFTREVARQCYDSDM 1023
            P  L  +EFPDF+++ +KP+Y S++V GKL+RAVK         +     VA   YD  +
Sbjct: 971  PYALKPREFPDFLERFEKPTYISESVFGKLYRAVKSSLAQRKPEAESEDTVA---YDVTL 1030

Query: 1024 EIEGYEDYVDDAIYYKNNYDYKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRD----- 1083
            E  G+E +++ A  +++ Y  KL +L+ YYG  +E EIL+G + T  K     RD     
Sbjct: 1031 EEAGFESFIETAKAHRDMYGEKLTSLMIYYGAANEEEILTGILKT--KEMYLARDNRRYG 1090

Query: 1084 --AEAINLAIRSLRKEARTWFNERESGPDSDSVLGLDDLFAKASAWYHVTYHPSYWGSYN 1143
               + I L+++ L KEA  WF +          L        ASAWY+VTY+P      N
Sbjct: 1091 DMKDRITLSVKDLHKEAMGWFEKSCEDEQQKKKL--------ASAWYYVTYNP------N 1132

Query: 1144 EDMNRDHYLSFPWCVYDKLMQIKENNVRRKQRASRLAALDS 1163
                +  +LSFPW V D L+ IK  N +R+    + + L S
Sbjct: 1151 HRDEKLTFLSFPWIVGDVLLDIKAENAQRQSVEEKTSGLVS 1132

BLAST of MS020748 vs. TAIR 10
Match: AT3G49500.1 (RNA-dependent RNA polymerase 6 )

HSP 1 Score: 589.0 bits (1517), Expect = 8.6e-168
Identity = 427/1204 (35.47%), Postives = 611/1204 (50.75%), Query Frame = 0

Query: 5    VHISGFPAGVTADAVRSYLEGRTGVGTVYALK----------------------IREPKK 64
            V I GF    TA  +  YLE   G+     LK                      I E KK
Sbjct: 15   VSIGGFGESTTAKQLTDYLEDEVGIVWRCRLKTSWTPPGSYPNFEIADTSNIPSIDEYKK 74

Query: 65   GGSRLYAFVQFTGSREAELIISLANQRIQRLWYGTSYLKAREADVDIIPK---------P 124
                 +AFV F     A   +  A Q           L  +   V + PK          
Sbjct: 75   --VEPHAFVHFAVFESAGRAMDAAGQ-------CNLILDGQPLKVSLGPKNPYSLNQRRR 134

Query: 125  RTYMHTLEDLTLCFGCQVSSEKFCVLW--EGRDVLVTFGFGMRKMNFRLSHNFVEYRFEL 184
             T  + L  +TL  G  VS + F V W  EG D LV       K  FR S  F  ++  +
Sbjct: 135  TTVPYKLAGITLEIGTLVSRDDFFVSWRAEGVDFLVDPFDNTCKFCFRKSTAF-SFKDAV 194

Query: 185  SYENIWRIELHHSRHQSMKYLVIQVLGSNSFPKLSHAILMLKIHQIIVMFFFSQLYGAPR 244
             +  I       +    ++ LV  +     +  L   +L+L            QL  +PR
Sbjct: 195  MHAVI-------NCDYKLELLVRDIQTVRQYKTLHGFVLIL------------QLASSPR 254

Query: 245  IYKKVESSSGSRRVFDDPLLNYFKEVPDDQWVRSTDFTPSSCIGQSSS--LCLKLHNCRE 304
            ++ +         V  D L +      DD W+R+TDFT    IG+  S  + +      +
Sbjct: 255  VWYRTADDDIYDTVPGDLLDD------DDPWIRTTDFTQVGAIGRCHSYRVLISPRYENK 314

Query: 305  LPNFGQNFAYYKETEDEFRFVDGGNGFSCSADLVPMVDSRPDIF----LPYKIIFKINAL 364
            L      F   +  E+  R+        C  +  P+ D    I     + ++I+F +N++
Sbjct: 315  LRTALDYFRMRRVQEERVRWPPRIRNEPCFGE--PVSDHFFCIHHKEGISFEIMFLVNSV 374

Query: 365  VQHGCISGPALDTSFYHLV--DPSRIKIEFVEHALDRLFHLKECEYKPYSWLTEQYKKYL 424
            +  G  +   L   F+ L+   P  + I  ++H    L   K   +  Y  L    +   
Sbjct: 375  LHRGVFNQFQLTERFFDLLRNQPKDVNIASLKH----LCTYKRPVFDAYKRLKLVQEWIQ 434

Query: 425  RRPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNVSNRVLRHFSRDIDNFLRVSFVDE 484
            + P    +      +  +RR+ ITP + Y   PEV +SNRVLR +    + FLRV+F+DE
Sbjct: 435  KNPKLLGSHEQSEDISEIRRLVITPTRAYCLPPEVELSNRVLRRYKAVAERFLRVTFMDE 494

Query: 485  EWDKMRSIDLLPRTSSTIKD-------RKTEIYKRILSVLKNGIVIGGKMFKFLAFSSSQ 544
                + S  L    +  +KD       +KT ++KR+ S+L +G  + G+ + FLAFS++Q
Sbjct: 495  SMQTINSNVLSYFVAPIVKDLTSSSFSQKTYVFKRVKSILTDGFKLCGRKYSFLAFSANQ 554

Query: 545  LRDNSLWMFATRPGLDAADIRAWMGDFRQIKNPAKYAARLGQSFGSSTETLSVARHEREI 604
            LRD S W FA       +DI+ WMG F+  KN AK AAR+G  F S+  T+ V  HE + 
Sbjct: 555  LRDRSAWFFAEDGKTRVSDIKTWMGKFKD-KNVAKCAARMGLCFSSTYATVDVMPHEVDT 614

Query: 605  -IPDIEVQHGEVKYVFSDGIGKISSDFAKKVSAKCGLKA-FTPSAFQIRYGGYKGVVAVD 664
             +PDIE ++G   YVFSDGIG I+ D A +V  K  L   ++P A+QIRY G+KGVVA  
Sbjct: 615  EVPDIE-RNG---YVFSDGIGTITPDLADEVMEKLKLDVHYSPCAYQIRYAGFKGVVARW 674

Query: 665  PYSSK--KLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLSTLGVRDEVFEKKQ 724
            P  S   +L+LR SM KF S +T L++  ++++QP FLNRQ+ITLLS LGV DE+F   Q
Sbjct: 675  PSKSDGIRLALRDSMKKFFSKHTILEICSWTRFQPGFLNRQIITLLSVLGVPDEIFWDMQ 734

Query: 725  HEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGYKPNVEPYLSMMLQAFRASK 784
               + +L+ IL D   A E L      E  N    ML  G+KP  EP+L  ML + R ++
Sbjct: 735  ESMLYKLNRILDDTDVAFEVLTASC-AEQGNTAAIMLSAGFKPKTEPHLRGMLSSVRIAQ 794

Query: 785  LLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTK-QSILSVTTSAFNMNSSEQ 844
            L  LR KSR+F+ +GR +MGCLDE G LE+GQ F+QVS    ++  S   S F    ++ 
Sbjct: 795  LWGLREKSRIFVTSGRWLMGCLDEAGILEHGQCFIQVSKPSIENCFSKHGSRFKETKTDL 854

Query: 845  RVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVDCVVFPQKGSRPHPNECSGSDLDG 904
             V +KG V +AKNPCLHPGD+R+L+AV+VPQL+HM DC++FPQKG RPH NE SGSDLDG
Sbjct: 855  EV-VKGYVAIAKNPCLHPGDVRILEAVDVPQLHHMYDCLIFPQKGDRPHTNEASGSDLDG 914

Query: 905  DIYFVCWDTELIPP--RQIPPMDYTPAPPTQLDRDVTIEDVQEYFVNYMVNDSLGIIANA 964
            D+YFV WD +LIPP  +  P M Y  A    L R V  +D+ ++F   + N+ LG I NA
Sbjct: 915  DLYFVAWDQKLIPPNRKSYPAMHYDAAEEKSLGRAVNHQDIIDFFARNLANEQLGTICNA 974

Query: 965  HTAFADKELFKARSSPCIELAKLFSIAVDFPKTGVPAIIPPELYVKEFPDFMDKPDKPSY 1024
            H   AD+  + A    C+ LA+L + AVDFPKTG    +P  L  K +PDFM K D  +Y
Sbjct: 975  HVVHADRSEYGAMDEECLLLAELAATAVDFPKTGKIVSMPFHLKPKLYPDFMGKEDYQTY 1034

Query: 1025 ESKNVIGKLFRAVKDIAPTTSHISL-FTREVARQCYDSDMEIEGYEDYVDDAIYYKNNYD 1084
            +S  ++G+L+R VK++    +  S   + + +   YD+ +EI G+ED + +A  +K  YD
Sbjct: 1035 KSNKILGRLYRRVKEVYDEDAEASSEESTDPSAIPYDAVLEIPGFEDLIPEAWGHKCLYD 1094

Query: 1085 YKLGNLLDYYGIKSEAEILSGNIMTMSKSFTKKRD--AEAINLAIRSLRKEARTWFNERE 1144
             +L  LL  Y ++ E EI++G+I +M K  +KK+    E +  +  SL+KE R  F E  
Sbjct: 1095 GQLIGLLGQYKVQKEEEIVTGHIWSMPKYTSKKQGELKERLKHSYNSLKKEFRKVFEETI 1154

BLAST of MS020748 vs. TAIR 10
Match: AT2G19910.1 (RNA-dependent RNA polymerase family protein )

HSP 1 Score: 114.4 bits (285), Expect = 6.2e-25
Identity = 120/474 (25.32%), Postives = 195/474 (41.14%), Query Frame = 0

Query: 570 VFSDGIGKISSDFAK---------------KVSAK---------CGLKAFTPSAFQIRYG 629
           + SDG G IS D A+                + +K         CG +      F+I Y 
Sbjct: 401 IHSDGTGYISEDLARMCPVNIFKGKSMRSNNIQSKNLNFEGQGPCGQEPPLLIQFRIFYN 460

Query: 630 GY--KGVVAVD-PYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQ---------PCFLNRQ 689
           GY  KG    +     + + +R SM K   D T  ++  ++  +            L+R 
Sbjct: 461 GYAVKGTFLTNKKLPPRTVQVRPSMIKVYEDRTLSNLSTFNSLEVVTTSNPPRKARLSRN 520

Query: 690 LITLLSTLGVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGY 749
           L+ LLS  GV ++ F       +++   I      A +A       + T    +M+  G 
Sbjct: 521 LVALLSYGGVPNDFFLNILRNTLEESKTIFYSERAAFKAAINYGDDQYT---ADMILVGI 580

Query: 750 KPNVEPYLSMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTK 809
            P  EPYL   L     ++   L+   R  I     +MG +D TG L+  ++ V +   +
Sbjct: 581 -PLDEPYLKDRLSYLLKTERNALKA-GRFPIDESYYIMGTVDPTGELKENEICVILHSGQ 640

Query: 810 QSILSVTTSAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVD----C 869
                                I G+V+V +NP LH GDI VLKA  V  L   V      
Sbjct: 641 ---------------------ISGDVLVYRNPGLHFGDIHVLKATYVKALEDYVGNAKFA 700

Query: 870 VVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIP---------PRQIPPMDYTPAPPTQ 929
           V FPQKG R   +E +G D DGD+YF+  + +L+              P   Y    P++
Sbjct: 701 VFFPQKGPRSLGDEIAGGDFDGDMYFISRNPKLLEHFKPSEPWVSSSKPSKIYCGRKPSE 760

Query: 930 LDRDVTIEDVQEYFV--NYMVNDSLGIIANAHTAFAD----------KELFKARSSPCIE 983
           L  +   E++ + F+   +   D +G+ A+      D          KE ++ R    ++
Sbjct: 761 LSEEELEEELFKMFLKARFCKRDVIGMAADCWLGIMDPFLTLGDESAKEKYE-RKKNILK 820

BLAST of MS020748 vs. TAIR 10
Match: AT2G19930.1 (RNA-dependent RNA polymerase family protein )

HSP 1 Score: 105.9 bits (263), Expect = 2.2e-22
Identity = 129/491 (26.27%), Postives = 209/491 (42.57%), Query Frame = 0

Query: 570  VFSDGIGKISSDFAKK-----VSAKCGLKAFTPSA----------FQIRYGGY--KG--- 629
            + SDG G IS D A+         KC        A          F++ Y GY  KG   
Sbjct: 397  IHSDGTGYISEDLARMCPLNIFKGKCLRSESIQEACYQDPPLLIQFRMFYDGYAVKGTFL 456

Query: 630  --------VVAVDPYSSKKLSLRKSMCKFESDNTKLDVLGYSKYQPCFLNRQLITLLSTL 689
                     V V P S  K+S   S+  F + N    V   +  +   L++ L+ LLS  
Sbjct: 457  LNKKLCPRTVQVRP-SMIKVSKDPSLSNFSTFNALEVVTTSNPPKRTKLSKNLVALLSYG 516

Query: 690  GVRDEVFEKKQHEAVKQLDAILTDPLKAQEALDLMAPGENTNVLKEMLKCGYKPNVEPYL 749
            G+ +E F       +++  +I  +   A  A       ++ N   +M+  G  P  EP+L
Sbjct: 517  GIPNEFFLDILLNTLEESKSIFYNKRAALNAALNYGEMDDQNA-AQMILVGI-PLDEPHL 576

Query: 750  SMMLQAFRASKLLDLRTKSRMFIPNGRAMMGCLDETGSLEYGQVFVQVSGTKQSILSVTT 809
               L     ++  DL+   ++ +     +MG +D TG+L+  +V V +   +        
Sbjct: 577  KNYLSILLKTEKNDLKA-GKLPVTESYYLMGTVDPTGALKEDEVCVILESGQ-------- 636

Query: 810  SAFNMNSSEQRVAIKGNVVVAKNPCLHPGDIRVLKAVNVPQLYHMVD----CVVFPQKGS 869
                         I G V+V +NP LH GDI +LKA  V  L   V      V FPQKG 
Sbjct: 637  -------------ISGEVLVYRNPGLHFGDIHILKATYVKALEEYVGNSKFAVFFPQKGP 696

Query: 870  RPHPNECSGSDLDGDIYFVCWDTELI---PPRQIPPMDYTP-------APPTQLDRDVTI 929
            R   +E +G D DGD+YF+  + EL+    P + P +  TP         P+QL  +   
Sbjct: 697  RSLGDEIAGGDFDGDMYFISRNPELLENFKPSE-PWVSLTPPSKSNSGRAPSQLSPEELE 756

Query: 930  EDVQEYFV--NYMVNDSLGIIANAHTAFADKELF--------KAR-SSPCIELAKLFSIA 989
            E++ E F+   +  ++ +GI A++     D+ L         KA      +EL  ++  A
Sbjct: 757  EELFEMFLTAGFHASNVIGIAADSWLTIMDRFLILGDDRAEEKAEMKKKMLELIDIYYDA 816

Query: 990  VDFPKTGVPAIIPPELYVKEFPDFMDKPDKPSYESKNVIGKLFRAVKDIAPTTSHISLFT 1008
            +D PK G    +P +L    FP +M++  K  ++S +++G +F  VK  + TT   S  +
Sbjct: 817  LDAPKKGDKVYLPNKLKPDIFPHYMERDKK--FQSTSILGLIFDFVK--SQTTEEPSPSS 857

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022146805.10.0e+0095.90probable RNA-dependent RNA polymerase 1 isoform X1 [Momordica charantia][more]
XP_022146806.10.0e+0093.09probable RNA-dependent RNA polymerase 1 isoform X2 [Momordica charantia][more]
XP_038891504.10.0e+0079.76RNA-dependent RNA polymerase 1 isoform X1 [Benincasa hispida] >XP_038891505.1 RN... [more]
XP_022943173.10.0e+0079.91RNA-dependent RNA polymerase 1-like isoform X2 [Cucurbita moschata] >XP_02294317... [more]
XP_022943172.10.0e+0079.91RNA-dependent RNA polymerase 1-like isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9LQV20.0e+0060.87RNA-dependent RNA polymerase 1 OS=Arabidopsis thaliana OX=3702 GN=RDR1 PE=2 SV=1[more]
Q0DXS31.5e-28666.80Probable RNA-dependent RNA polymerase 1 OS=Oryza sativa subsp. japonica OX=39947... [more]
O825041.2e-21739.46RNA-dependent RNA polymerase 2 OS=Arabidopsis thaliana OX=3702 GN=RDR2 PE=1 SV=1[more]
Q7XM312.7e-20637.00Probable RNA-dependent RNA polymerase 2 OS=Oryza sativa subsp. japonica OX=39947... [more]
Q8LHH94.9e-16839.05Probable RNA-dependent RNA polymerase SHL2 OS=Oryza sativa subsp. japonica OX=39... [more]
Match NameE-valueIdentityDescription
A0A6J1CYE00.0e+0095.90RNA-dependent RNA polymerase OS=Momordica charantia OX=3673 GN=LOC111015923 PE=3... [more]
A0A6J1D0H10.0e+0093.09RNA-dependent RNA polymerase OS=Momordica charantia OX=3673 GN=LOC111015923 PE=3... [more]
A0A6J1FXH50.0e+0079.91RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111447985 PE=3 ... [more]
A0A6J1FSA20.0e+0079.91RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111447985 PE=3 ... [more]
A0A6J1K2P90.0e+0079.74RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111490498 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT1G14790.10.0e+0060.87RNA-dependent RNA polymerase 1 [more]
AT4G11130.18.2e-21939.46RNA-dependent RNA polymerase 2 [more]
AT3G49500.18.6e-16835.47RNA-dependent RNA polymerase 6 [more]
AT2G19910.16.2e-2525.32RNA-dependent RNA polymerase family protein [more]
AT2G19930.12.2e-2226.27RNA-dependent RNA polymerase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3D3.30.70.330coord: 1..88
e-value: 2.2E-5
score: 26.5
IPR007855RNA-dependent RNA polymerase, eukaryotic-typePFAMPF05183RdRPcoord: 406..982
e-value: 4.6E-188
score: 626.5
IPR007855RNA-dependent RNA polymerase, eukaryotic-typePANTHERPTHR23079RNA-DEPENDENT RNA POLYMERASEcoord: 2..1168
NoneNo IPR availablePANTHERPTHR23079:SF44RNA-DEPENDENT RNA POLYMERASEcoord: 2..1168
NoneNo IPR availableCDDcd00590RRM_SFcoord: 5..69
e-value: 7.77505E-7
score: 45.7589
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 3..92
score: 10.138468
IPR035979RNA-binding domain superfamilySUPERFAMILY54928RNA-binding domain, RBDcoord: 2..69

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS020748.1MS020748.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003968 RNA-directed 5'-3' RNA polymerase activity