MS020668 (gene) Bitter gourd (TR) v1

Overview


Name	MS020668
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	chitinase 2-like
Location	scaffold375: 2054800 .. 2055711 (-)
RNA-Seq Expression	MS020668
Synteny	MS020668

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTCTCGAATATTCTTCCTATTCTCACTTCTACAATTCCTAAGCGCCATACTTCCATCACTAGAGGCAGCCCCCAAAAATAACACACGACTCTTCAGAGAATACATAGGAGCTGATGGAAATGTAAGATTTTCAGACGTACCTGTTCATCCTGATGTAGAGTTCCACTTCATTCTCTCCTTTGCCATCGATTACACCGGTTTATCTTCTCCTTCTGCGACCAACGGCAAATTCAGAGTCTTCTGGGACGAACAAAACCTCTCGCCTTCTTCTGTTTCTTCCATCAAGGCTCGGCATTCGAACATCAAGTTTGCTGTCAGCCTCGGGGGTGATACGGTCGGCAAAGAATTTGCTTACTTCAAACCCAAATCTGTCAAATCTTGGGTTAAAAATGCTTTCAAGTCAATCTCTCGTATAGTGAAGGAGTATGATCTGGATGGGGTTGACATCGATTATGAACACTTCAAAGCAGACCCCGATACATTTACCGAGTGCATAGGGCAGCTCCTACTTCGTCTGAAACACCATAATGTCATCTCGTTTGCATCCATAGCACCGTTTGAGGATGACACTGTGCAGTCTCATTACTTAGCTTTGTGGAGGAAGTACGGACATCTAATAGACTATGTCAACTTCCAGTTCTATTCCTATGATAAAGGTACCACAGTTTCTCAGTTTGTGAATCATTTTAAGACCCAAAGCTCCAATTATCAAGGTGGAAATGTTCTAGCAAGCTTTGCTACAGATGGAAGTGGCGGTTTGACACCGGAAAAAGGGTTCTTTAAAGCATGTAGCATCCTTCAAAGCAGGGGCGAACTTCATGGTATATTTGTTTGGTCAGCAGATGACTCAAAGAAGAACAATTTTCGTCATGAGAAACAAGCACAAAACCTTTTGGCCAACGCAGAA

mRNA sequence

ATGGGTTCTCGAATATTCTTCCTATTCTCACTTCTACAATTCCTAAGCGCCATACTTCCATCACTAGAGGCAGCCCCCAAAAATAACACACGACTCTTCAGAGAATACATAGGAGCTGATGGAAATGTAAGATTTTCAGACGTACCTGTTCATCCTGATGTAGAGTTCCACTTCATTCTCTCCTTTGCCATCGATTACACCGGTTTATCTTCTCCTTCTGCGACCAACGGCAAATTCAGAGTCTTCTGGGACGAACAAAACCTCTCGCCTTCTTCTGTTTCTTCCATCAAGGCTCGGCATTCGAACATCAAGTTTGCTGTCAGCCTCGGGGGTGATACGGTCGGCAAAGAATTTGCTTACTTCAAACCCAAATCTGTCAAATCTTGGGTTAAAAATGCTTTCAAGTCAATCTCTCGTATAGTGAAGGAGTATGATCTGGATGGGGTTGACATCGATTATGAACACTTCAAAGCAGACCCCGATACATTTACCGAGTGCATAGGGCAGCTCCTACTTCGTCTGAAACACCATAATGTCATCTCGTTTGCATCCATAGCACCGTTTGAGGATGACACTGTGCAGTCTCATTACTTAGCTTTGTGGAGGAAGTACGGACATCTAATAGACTATGTCAACTTCCAGTTCTATTCCTATGATAAAGGTACCACAGTTTCTCAGTTTGTGAATCATTTTAAGACCCAAAGCTCCAATTATCAAGGTGGAAATGTTCTAGCAAGCTTTGCTACAGATGGAAGTGGCGGTTTGACACCGGAAAAAGGGTTCTTTAAAGCATGTAGCATCCTTCAAAGCAGGGGCGAACTTCATGGTATATTTGTTTGGTCAGCAGATGACTCAAAGAAGAACAATTTTCGTCATGAGAAACAAGCACAAAACCTTTTGGCCAACGCAGAA

Coding sequence (CDS)

ATGGGTTCTCGAATATTCTTCCTATTCTCACTTCTACAATTCCTAAGCGCCATACTTCCATCACTAGAGGCAGCCCCCAAAAATAACACACGACTCTTCAGAGAATACATAGGAGCTGATGGAAATGTAAGATTTTCAGACGTACCTGTTCATCCTGATGTAGAGTTCCACTTCATTCTCTCCTTTGCCATCGATTACACCGGTTTATCTTCTCCTTCTGCGACCAACGGCAAATTCAGAGTCTTCTGGGACGAACAAAACCTCTCGCCTTCTTCTGTTTCTTCCATCAAGGCTCGGCATTCGAACATCAAGTTTGCTGTCAGCCTCGGGGGTGATACGGTCGGCAAAGAATTTGCTTACTTCAAACCCAAATCTGTCAAATCTTGGGTTAAAAATGCTTTCAAGTCAATCTCTCGTATAGTGAAGGAGTATGATCTGGATGGGGTTGACATCGATTATGAACACTTCAAAGCAGACCCCGATACATTTACCGAGTGCATAGGGCAGCTCCTACTTCGTCTGAAACACCATAATGTCATCTCGTTTGCATCCATAGCACCGTTTGAGGATGACACTGTGCAGTCTCATTACTTAGCTTTGTGGAGGAAGTACGGACATCTAATAGACTATGTCAACTTCCAGTTCTATTCCTATGATAAAGGTACCACAGTTTCTCAGTTTGTGAATCATTTTAAGACCCAAAGCTCCAATTATCAAGGTGGAAATGTTCTAGCAAGCTTTGCTACAGATGGAAGTGGCGGTTTGACACCGGAAAAAGGGTTCTTTAAAGCATGTAGCATCCTTCAAAGCAGGGGCGAACTTCATGGTATATTTGTTTGGTCAGCAGATGACTCAAAGAAGAACAATTTTCGTCATGAGAAACAAGCACAAAACCTTTTGGCCAACGCAGAA

Protein sequence

MGSRIFFLFSLLQFLSAILPSLEAAPKNNTRLFREYIGADGNVRFSDVPVHPDVEFHFILSFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKEFAYFKPKSVKSWVKNAFKSISRIVKEYDLDGVDIDYEHFKADPDTFTECIGQLLLRLKHHNVISFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNYQGGNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQNLLANAE

Homology

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BLAST of MS020668 vs. NCBI nr
Match: XP_022146270.1 (chitinase 2-like [Momordica charantia])

HSP 1 Score: 616.7 bits (1589), Expect = 1.1e-172
Identity = 303/304 (99.67%), Postives = 303/304 (99.67%), Query Frame = 0


Query: 1   MGSRIFFLFSLLQFLSAILPSLEAAPKNNTRLFREYIGADGNVRFSDVPVHPDVEFHFIL 60
           MGSRIFFLFSLLQFLSAILPSLEAAPKNNTRLFREYIGADGNVRFSDVPVHPDVEFHFIL
Sbjct: 19  MGSRIFFLFSLLQFLSAILPSLEAAPKNNTRLFREYIGADGNVRFSDVPVHPDVEFHFIL 78

Query: 61  SFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKEFAY 120
           SFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKEFAY
Sbjct: 79  SFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKEFAY 138

Query: 121 FKPKSVKSWVKNAFKSISRIVKEYDLDGVDIDYEHFKADPDTFTECIGQLLLRLKHHNVI 180
           FKPKSVKSWVKNAFKSISRIVKEY LDGVDIDYEHFKADPDTFTECIGQLLLRLKHHNVI
Sbjct: 139 FKPKSVKSWVKNAFKSISRIVKEYHLDGVDIDYEHFKADPDTFTECIGQLLLRLKHHNVI 198

Query: 181 SFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNYQG 240
           SFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNYQG
Sbjct: 199 SFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNYQG 258

Query: 241 GNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQNLL 300
           GNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQNLL
Sbjct: 259 GNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQNLL 318

Query: 301 ANAE 305
           ANAE
Sbjct: 319 ANAE 322

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BLAST of MS020668 vs. NCBI nr
Match: XP_038902883.1 (chitinase 2-like [Benincasa hispida])

HSP 1 Score: 530.8 bits (1366), Expect = 7.8e-147
Identity = 263/307 (85.67%), Postives = 284/307 (92.51%), Query Frame = 0


Query: 1   MGSRIFFLFSLLQFLS--AILPSLEAAPKNNTRLFREYIGADG-NVRFSDVPVHPDVEFH 60
           MGSRIFFL+S LQFLS  AILPSL+AAP     LFREYIGA+G N+RFSDVP+H DVEFH
Sbjct: 1   MGSRIFFLYSFLQFLSVFAILPSLKAAP-----LFREYIGAEGINIRFSDVPIHSDVEFH 60

Query: 61  FILSFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKE 120
           FILSFAIDYTG SSPSATNGKFRVFWDE+NLSPSSVSSIKA+HSN+KFA+SLGGDTVGKE
Sbjct: 61  FILSFAIDYTGSSSPSATNGKFRVFWDEENLSPSSVSSIKAKHSNVKFALSLGGDTVGKE 120

Query: 121 FAYFKPKSVKSWVKNAFKSISRIVKEYDLDGVDIDYEHFKADPDTFTECIGQLLLRLKHH 180
           FA+FKPKS+ SWV NAFKSISRIVK+YDLDGVDIDYEHFKA PD FTECIGQLLLRLK H
Sbjct: 121 FAFFKPKSITSWVNNAFKSISRIVKKYDLDGVDIDYEHFKAHPDIFTECIGQLLLRLKQH 180

Query: 181 NVISFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSN 240
           N+ISFASIAPFEDDTVQSHY+ALWRKYG LIDYVNFQFY+Y KGTTVSQF+NHF+TQSSN
Sbjct: 181 NLISFASIAPFEDDTVQSHYMALWRKYGDLIDYVNFQFYAYGKGTTVSQFLNHFETQSSN 240

Query: 241 YQGGNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQ 300
           Y+GG VLASFATDGSGGLTPEKGFFKACS LQSRG LHGIFVWSADDSKKN+F +E QAQ
Sbjct: 241 YEGGKVLASFATDGSGGLTPEKGFFKACSTLQSRGNLHGIFVWSADDSKKNDFHNEIQAQ 300

Query: 301 NLLANAE 305
           NLLANA+
Sbjct: 301 NLLANAK 302

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BLAST of MS020668 vs. NCBI nr
Match: XP_022944087.1 (chitinase 2-like [Cucurbita moschata])

HSP 1 Score: 524.2 bits (1349), Expect = 7.3e-145
Identity = 258/306 (84.31%), Postives = 278/306 (90.85%), Query Frame = 0


Query: 1   MGS-RIFFLFSLLQFLSAILPSLEAAPKNNTRLFREYIGADG-NVRFSDVPVHPDVEFHF 60
           MGS +IFFLFS L  L   LPSLEA P +NTRLFREYIGADG NVRFSDVP+HPDVEFHF
Sbjct: 18  MGSAQIFFLFSFLPLLFPTLPSLEATPLDNTRLFREYIGADGNNVRFSDVPIHPDVEFHF 77

Query: 61  ILSFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKEF 120
           ILSFAIDYT  SSPSATNGKFRVFWDEQ LSPSSVSSIKA+HSN+KFA+SLGGDT GK+F
Sbjct: 78  ILSFAIDYTASSSPSATNGKFRVFWDEQILSPSSVSSIKAQHSNVKFALSLGGDTAGKQF 137

Query: 121 AYFKPKSVKSWVKNAFKSISRIVKEYDLDGVDIDYEHFKADPDTFTECIGQLLLRLKHHN 180
           AYFKPKS+ SWVKNAF+SISRIVKEYD+DGVDIDYEHFKA PD FTECIG+LLLRLK  N
Sbjct: 138 AYFKPKSITSWVKNAFESISRIVKEYDIDGVDIDYEHFKAHPDVFTECIGKLLLRLKRQN 197

Query: 181 VISFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNY 240
           VISFASIAPF+DD VQ+HYLALWRKYG LIDYVNFQFY+Y KGTTVSQF+NHF+TQ+SNY
Sbjct: 198 VISFASIAPFDDDAVQTHYLALWRKYGDLIDYVNFQFYAYGKGTTVSQFLNHFETQTSNY 257

Query: 241 QGGNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQN 300
           QGG VLASFATDGSGGLTPEKGFFKAC  LQSRG+LHGIFVWSADDSKKN F +E QAQN
Sbjct: 258 QGGKVLASFATDGSGGLTPEKGFFKACRTLQSRGKLHGIFVWSADDSKKNKFHNEIQAQN 317

Query: 301 LLANAE 305
           LLANA+
Sbjct: 318 LLANAD 323

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BLAST of MS020668 vs. NCBI nr
Match: KAG6570744.1 (hypothetical protein SDJN03_29659, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 524.2 bits (1349), Expect = 7.3e-145
Identity = 258/306 (84.31%), Postives = 278/306 (90.85%), Query Frame = 0


Query: 1   MGS-RIFFLFSLLQFLSAILPSLEAAPKNNTRLFREYIGADG-NVRFSDVPVHPDVEFHF 60
           MGS +I FLFS LQ L   LPSLEA P +NTRLFREYIGADG NVRFSDVP+HPDVEFHF
Sbjct: 1   MGSAQIIFLFSFLQLLFPTLPSLEATPLDNTRLFREYIGADGNNVRFSDVPIHPDVEFHF 60

Query: 61  ILSFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKEF 120
           ILSFAIDYT  SSPSATNGKFRVFWDEQ LSPSSVSSIKA+HSN+KFA+SLGGDT GK+F
Sbjct: 61  ILSFAIDYTAASSPSATNGKFRVFWDEQILSPSSVSSIKAQHSNVKFALSLGGDTAGKQF 120

Query: 121 AYFKPKSVKSWVKNAFKSISRIVKEYDLDGVDIDYEHFKADPDTFTECIGQLLLRLKHHN 180
           AYFKPKS+ SWVKNAF+SISRIVKEYDLDGVDIDYEHFKA PD FTEC+G+LLLRLK  N
Sbjct: 121 AYFKPKSITSWVKNAFESISRIVKEYDLDGVDIDYEHFKAHPDVFTECMGKLLLRLKRQN 180

Query: 181 VISFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNY 240
           VISFASIAPF+DD VQ+HYLALWRKYG LIDYVNFQFY+Y KGTTVSQF+NHF+TQ+SNY
Sbjct: 181 VISFASIAPFDDDAVQTHYLALWRKYGDLIDYVNFQFYAYGKGTTVSQFLNHFETQTSNY 240

Query: 241 QGGNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQN 300
           QGG VLASFATDGSGGLTPEKGFFKAC  LQSRG+LHGIFVWSADDSKKN F +E QAQN
Sbjct: 241 QGGKVLASFATDGSGGLTPEKGFFKACRTLQSRGKLHGIFVWSADDSKKNKFHNEIQAQN 300

Query: 301 LLANAE 305
           LLANA+
Sbjct: 301 LLANAD 306

[Close]

BLAST of MS020668 vs. NCBI nr
Match: XP_022986910.1 (chitinase 2-like [Cucurbita maxima])

HSP 1 Score: 523.9 bits (1348), Expect = 9.5e-145
Identity = 259/306 (84.64%), Postives = 278/306 (90.85%), Query Frame = 0


Query: 1   MGS-RIFFLFSLLQFLSAILPSLEAAPKNNTRLFREYIGADG-NVRFSDVPVHPDVEFHF 60
           MGS +IFFLFS LQ L   LPSLEA P +NTRLFREYIGADG NVRFSDVP+HP+VE HF
Sbjct: 1   MGSAQIFFLFSFLQLLFPTLPSLEATPLDNTRLFREYIGADGNNVRFSDVPIHPEVELHF 60

Query: 61  ILSFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKEF 120
           ILSFAIDYT  SSPSATN KFRVFWD+Q LSPSSVSSIKA+HSNIKFA+SLGGDTVGK+F
Sbjct: 61  ILSFAIDYTASSSPSATNEKFRVFWDDQILSPSSVSSIKAQHSNIKFALSLGGDTVGKQF 120

Query: 121 AYFKPKSVKSWVKNAFKSISRIVKEYDLDGVDIDYEHFKADPDTFTECIGQLLLRLKHHN 180
           AYFKPKS+ SWVKNAF+SISRIVKEYDLDGVDIDYEHFKA PD FTECIG+LLLRLKH N
Sbjct: 121 AYFKPKSITSWVKNAFESISRIVKEYDLDGVDIDYEHFKAHPDVFTECIGKLLLRLKHQN 180

Query: 181 VISFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNY 240
           VISFASIAPF+DD VQSHYLALWRKYG LIDYVNFQFY+Y KGTTVSQF+NHF+TQ+SNY
Sbjct: 181 VISFASIAPFDDDAVQSHYLALWRKYGDLIDYVNFQFYAYGKGTTVSQFLNHFETQTSNY 240

Query: 241 QGGNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQN 300
           QGG VLASFATDGSGGLTPEKGFFKAC  LQSRG+LHGIFVWSADDSKKN F +E QAQN
Sbjct: 241 QGGKVLASFATDGSGGLTPEKGFFKACRTLQSRGKLHGIFVWSADDSKKNKFHNEIQAQN 300

Query: 301 LLANAE 305
           LLAN +
Sbjct: 301 LLANTD 306

[Close]

BLAST of MS020668 vs. ExPASy Swiss-Prot
Match: Q9SLP4 (Chitinase 1 OS=Tulipa saxatilis subsp. bakeri OX=110455 PE=1 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 2.0e-105
Identity = 183/295 (62.03%), Postives = 231/295 (78.31%), Query Frame = 0


Query: 11  LLQFLSAILPSLEAAPKNNTRLFREYIGADGN-VRFSDVPVHPDVEFHFILSFAIDYTGL 70
           LL F + + P L   P  +  +FREYIG+  N V+FSDVP++PDV+FHFIL+FAIDYT  
Sbjct: 10  LLLFFALLSPLL---PLTSALVFREYIGSQFNDVKFSDVPINPDVDFHFILAFAIDYTSG 69

Query: 71  SSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKEFAYFKPKSVKSW 130
           SSP+ TNG F+ FWD  NLSPS V+++K  HSN+K ++SLGGD+VG +  +F P SV SW
Sbjct: 70  SSPTPTNGNFKPFWDTNNLSPSQVAAVKRTHSNVKVSLSLGGDSVGGKNVFFSPSSVSSW 129

Query: 131 VKNAFKSISRIVKEYDLDGVDIDYEHFKADPDTFTECIGQLLLRLKHHNVISFASIAPFE 190
           V+NA  S++RI+K+Y LDG+DIDYEHFK DP+TF ECIGQL+ RLK + V+SF SIAPF+
Sbjct: 130 VENAVSSLTRIIKQYHLDGIDIDYEHFKGDPNTFAECIGQLVTRLKKNEVVSFVSIAPFD 189

Query: 191 DDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNYQGGNVLASFAT 250
           D  VQSHY ALW KYGH IDYVNFQFY+Y   T+V QF+ +F+ QSSNY GG VL SF+T
Sbjct: 190 DAQVQSHYQALWEKYGHQIDYVNFQFYAYSARTSVEQFLKYFEEQSSNYHGGKVLVSFST 249

Query: 251 DGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNN--FRHEKQAQNLLAN 303
           D SGGL P+ GFF+ACSIL+ +G+LHGIFVWSADDS  +N  FR+E QAQ++LA+
Sbjct: 250 DSSGGLKPDNGFFRACSILKKQGKLHGIFVWSADDSLMSNNVFRYEMQAQSMLAS 301

[Close]

BLAST of MS020668 vs. ExPASy Swiss-Prot
Match: Q7M443 (Chitinase 2 OS=Tulipa saxatilis subsp. bakeri OX=110455 PE=1 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 1.9e-100
Identity = 175/274 (63.87%), Postives = 215/274 (78.47%), Query Frame = 0


Query: 32  LFREYIGADGN-VRFSDVPVHPDVEFHFILSFAIDYTGLSSPSATNGKFRVFWDEQNLSP 91
           LFREYIGA  N V+FSDVP++P+V+FHFIL+FAIDYT  SSP+ TNG F  FWD  NLSP
Sbjct: 2   LFREYIGAQFNDVKFSDVPINPNVDFHFILAFAIDYTSGSSPTPTNGNFNPFWDTNNLSP 61

Query: 92  SSVSSIKARHSNIKFAVSLGGDTVGKEFAYFKPKSVKSWVKNAFKSISRIVKEYDLDGVD 151
           S V++IK  ++N+K +VSLGG++VG E  +F P SV SWV NA  S+++I+K+Y LDG+D
Sbjct: 62  SQVAAIKRTYNNVKVSVSLGGNSVGGERVFFNPSSVSSWVDNAVSSLTKIIKQYHLDGID 121

Query: 152 IDYEHFKADPDTFTECIGQLLLRLKHHNVISFASIAPFEDDTVQSHYLALWRKYGHLIDY 211
           IDYEHFK DP+TF ECIGQL+ RLK + V+SF SIAPF+D  VQSHY ALW KYGH IDY
Sbjct: 122 IDYEHFKGDPNTFAECIGQLVTRLKKNGVVSFVSIAPFDDAQVQSHYQALWEKYGHQIDY 181

Query: 212 VNFQFYSYDKGTTVSQFVNHFKTQSSNYQGGNVLASFATDGSGGLTPEKGFFKACSILQS 271
           VNFQFY Y    +V QF+ +F+ Q SNY GG VL SF+TD SGGL P  GFF ACSIL+ 
Sbjct: 182 VNFQFYVYSSRMSVEQFLKYFEMQRSNYPGGKVLVSFSTDNSGGLKPRNGFFDACSILKK 241

Query: 272 RGELHGIFVWSADDSKKNN--FRHEKQAQNLLAN 303
           +G+LHGIFVWSADDS  +N  F++E QAQ+LLA+
Sbjct: 242 QGKLHGIFVWSADDSLMSNDVFKYEMQAQSLLAS 275

[Close]

BLAST of MS020668 vs. ExPASy Swiss-Prot
Match: P39657 (RuBisCO-associated protein OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 2.2e-19
Identity = 58/187 (31.02%), Postives = 89/187 (47.59%), Query Frame = 0


Query: 31  RLFREYIGADGNVRFSDVPVHPDVEFHFILSFAIDYTGLSSPSATNGKFRVFWDEQNLSP 90
           ++FRE+   D  +  + V      EF   LS A DY G +S   TNGKF  +WD + ++P
Sbjct: 6   KVFREFTSDDSFL--NQVIPENITEFQVTLSLARDYDGNNS---TNGKFIPYWDTEKVTP 65

Query: 91  SSVSSIKARHS----NIKFAVSLGGDTVGKEFAYFKPKSVKSWVKNAFKSISRIVKEYDL 150
             +   K ++      +K  VS+G       F      + ++WV  A  S+  I+K Y+L
Sbjct: 66  EVIKKFKKKYEPTALRVKVLVSIGNKNKQFPFTIGSDSNSEAWVSEATASLKSIIKTYNL 125

Query: 151 DGVDIDYEHFKADPDTFTECIGQLLLRLKHHNVISFASIAPFEDDTVQSHYLALWRKYGH 210
           DG+D+ YE   A+   F   +G L+  LK + +I+ AS A   D      Y  L+ +Y  
Sbjct: 126 DGIDVSYEDIAANEADFVNSVGGLVRNLKQNKLITVASFATSADAANNKFYNLLYAEYAT 185

Query: 211 LIDYVNF 214
             D V F
Sbjct: 186 FFDTVVF 187

[Close]

BLAST of MS020668 vs. ExPASy TrEMBL
Match: A0A6J1CWU0 (chitinase 2-like OS=Momordica charantia OX=3673 GN=LOC111015520 PE=3 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 5.2e-173
Identity = 303/304 (99.67%), Postives = 303/304 (99.67%), Query Frame = 0


Query: 1   MGSRIFFLFSLLQFLSAILPSLEAAPKNNTRLFREYIGADGNVRFSDVPVHPDVEFHFIL 60
           MGSRIFFLFSLLQFLSAILPSLEAAPKNNTRLFREYIGADGNVRFSDVPVHPDVEFHFIL
Sbjct: 19  MGSRIFFLFSLLQFLSAILPSLEAAPKNNTRLFREYIGADGNVRFSDVPVHPDVEFHFIL 78

Query: 61  SFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKEFAY 120
           SFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKEFAY
Sbjct: 79  SFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKEFAY 138

Query: 121 FKPKSVKSWVKNAFKSISRIVKEYDLDGVDIDYEHFKADPDTFTECIGQLLLRLKHHNVI 180
           FKPKSVKSWVKNAFKSISRIVKEY LDGVDIDYEHFKADPDTFTECIGQLLLRLKHHNVI
Sbjct: 139 FKPKSVKSWVKNAFKSISRIVKEYHLDGVDIDYEHFKADPDTFTECIGQLLLRLKHHNVI 198

Query: 181 SFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNYQG 240
           SFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNYQG
Sbjct: 199 SFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNYQG 258

Query: 241 GNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQNLL 300
           GNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQNLL
Sbjct: 259 GNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQNLL 318

Query: 301 ANAE 305
           ANAE
Sbjct: 319 ANAE 322

[Close]

BLAST of MS020668 vs. ExPASy TrEMBL
Match: A0A6J1FUT9 (chitinase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111448631 PE=3 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 3.5e-145
Identity = 258/306 (84.31%), Postives = 278/306 (90.85%), Query Frame = 0


Query: 1   MGS-RIFFLFSLLQFLSAILPSLEAAPKNNTRLFREYIGADG-NVRFSDVPVHPDVEFHF 60
           MGS +IFFLFS L  L   LPSLEA P +NTRLFREYIGADG NVRFSDVP+HPDVEFHF
Sbjct: 18  MGSAQIFFLFSFLPLLFPTLPSLEATPLDNTRLFREYIGADGNNVRFSDVPIHPDVEFHF 77

Query: 61  ILSFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKEF 120
           ILSFAIDYT  SSPSATNGKFRVFWDEQ LSPSSVSSIKA+HSN+KFA+SLGGDT GK+F
Sbjct: 78  ILSFAIDYTASSSPSATNGKFRVFWDEQILSPSSVSSIKAQHSNVKFALSLGGDTAGKQF 137

Query: 121 AYFKPKSVKSWVKNAFKSISRIVKEYDLDGVDIDYEHFKADPDTFTECIGQLLLRLKHHN 180
           AYFKPKS+ SWVKNAF+SISRIVKEYD+DGVDIDYEHFKA PD FTECIG+LLLRLK  N
Sbjct: 138 AYFKPKSITSWVKNAFESISRIVKEYDIDGVDIDYEHFKAHPDVFTECIGKLLLRLKRQN 197

Query: 181 VISFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNY 240
           VISFASIAPF+DD VQ+HYLALWRKYG LIDYVNFQFY+Y KGTTVSQF+NHF+TQ+SNY
Sbjct: 198 VISFASIAPFDDDAVQTHYLALWRKYGDLIDYVNFQFYAYGKGTTVSQFLNHFETQTSNY 257

Query: 241 QGGNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQN 300
           QGG VLASFATDGSGGLTPEKGFFKAC  LQSRG+LHGIFVWSADDSKKN F +E QAQN
Sbjct: 258 QGGKVLASFATDGSGGLTPEKGFFKACRTLQSRGKLHGIFVWSADDSKKNKFHNEIQAQN 317

Query: 301 LLANAE 305
           LLANA+
Sbjct: 318 LLANAD 323

[Close]

BLAST of MS020668 vs. ExPASy TrEMBL
Match: A0A6J1JFC6 (chitinase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111484508 PE=3 SV=1)

HSP 1 Score: 523.9 bits (1348), Expect = 4.6e-145
Identity = 259/306 (84.64%), Postives = 278/306 (90.85%), Query Frame = 0


Query: 1   MGS-RIFFLFSLLQFLSAILPSLEAAPKNNTRLFREYIGADG-NVRFSDVPVHPDVEFHF 60
           MGS +IFFLFS LQ L   LPSLEA P +NTRLFREYIGADG NVRFSDVP+HP+VE HF
Sbjct: 1   MGSAQIFFLFSFLQLLFPTLPSLEATPLDNTRLFREYIGADGNNVRFSDVPIHPEVELHF 60

Query: 61  ILSFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKEF 120
           ILSFAIDYT  SSPSATN KFRVFWD+Q LSPSSVSSIKA+HSNIKFA+SLGGDTVGK+F
Sbjct: 61  ILSFAIDYTASSSPSATNEKFRVFWDDQILSPSSVSSIKAQHSNIKFALSLGGDTVGKQF 120

Query: 121 AYFKPKSVKSWVKNAFKSISRIVKEYDLDGVDIDYEHFKADPDTFTECIGQLLLRLKHHN 180
           AYFKPKS+ SWVKNAF+SISRIVKEYDLDGVDIDYEHFKA PD FTECIG+LLLRLKH N
Sbjct: 121 AYFKPKSITSWVKNAFESISRIVKEYDLDGVDIDYEHFKAHPDVFTECIGKLLLRLKHQN 180

Query: 181 VISFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNY 240
           VISFASIAPF+DD VQSHYLALWRKYG LIDYVNFQFY+Y KGTTVSQF+NHF+TQ+SNY
Sbjct: 181 VISFASIAPFDDDAVQSHYLALWRKYGDLIDYVNFQFYAYGKGTTVSQFLNHFETQTSNY 240

Query: 241 QGGNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQN 300
           QGG VLASFATDGSGGLTPEKGFFKAC  LQSRG+LHGIFVWSADDSKKN F +E QAQN
Sbjct: 241 QGGKVLASFATDGSGGLTPEKGFFKACRTLQSRGKLHGIFVWSADDSKKNKFHNEIQAQN 300

Query: 301 LLANAE 305
           LLAN +
Sbjct: 301 LLANTD 306

[Close]

BLAST of MS020668 vs. ExPASy TrEMBL
Match: A0A5D3CQZ2 (Chitinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G00970 PE=3 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 7.8e-145
Identity = 258/306 (84.31%), Postives = 277/306 (90.52%), Query Frame = 0


Query: 1   MGSRIFFLFSLLQFLSAILPSLEAAPKNNTRLFREYIGA-DGNVRFSDVPVHPDVEFHFI 60
           MGSRIFFLFS L FL AILPS++A P  NTRLFREYIGA D NVRFSDVP+H DVEFHFI
Sbjct: 1   MGSRIFFLFSFLGFLFAILPSVKAEPLKNTRLFREYIGAEDKNVRFSDVPIHSDVEFHFI 60

Query: 61  LSFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKE-F 120
           LSFAIDYTG SSPS TNGKF+VFWDE+NL+PSS+SSIKA+HSN+KFA+SLGGDTVG   F
Sbjct: 61  LSFAIDYTGSSSPSPTNGKFKVFWDEKNLTPSSISSIKAKHSNVKFALSLGGDTVGDGIF 120

Query: 121 AYFKPKSVKSWVKNAFKSISRIVKEYDLDGVDIDYEHFKADPDTFTECIGQLLLRLKHHN 180
            +FKPKS+ SWV NAFKSIS IVKEYDLDGVDIDYEHFKA PDTFTECIGQLL RLK  N
Sbjct: 121 VFFKPKSITSWVNNAFKSISDIVKEYDLDGVDIDYEHFKASPDTFTECIGQLLQRLKSQN 180

Query: 181 VISFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNY 240
           +ISFASIAPF+DDTVQSHY+ALWRKYG LIDYVNFQFYSY KGTTVSQF+NHF TQSSNY
Sbjct: 181 IISFASIAPFDDDTVQSHYMALWRKYGDLIDYVNFQFYSYGKGTTVSQFLNHFDTQSSNY 240

Query: 241 QGGNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQN 300
           +GG VLASFATDGSGGL PEKGFFKAC+ LQSRGELHGIFVWSADDSKKNNFR+E QAQN
Sbjct: 241 EGGKVLASFATDGSGGLKPEKGFFKACNTLQSRGELHGIFVWSADDSKKNNFRNEIQAQN 300

Query: 301 LLANAE 305
           LLANAE
Sbjct: 301 LLANAE 306

[Close]

BLAST of MS020668 vs. ExPASy TrEMBL
Match: A0A1S3CLR6 (chitinase 2-like OS=Cucumis melo OX=3656 GN=LOC103501894 PE=3 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 7.8e-145
Identity = 258/306 (84.31%), Postives = 277/306 (90.52%), Query Frame = 0


Query: 1   MGSRIFFLFSLLQFLSAILPSLEAAPKNNTRLFREYIGA-DGNVRFSDVPVHPDVEFHFI 60
           MGSRIFFLFS L FL AILPS++A P  NTRLFREYIGA D NVRFSDVP+H DVEFHFI
Sbjct: 19  MGSRIFFLFSFLGFLFAILPSVKAEPLKNTRLFREYIGAEDKNVRFSDVPIHSDVEFHFI 78

Query: 61  LSFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKE-F 120
           LSFAIDYTG SSPS TNGKF+VFWDE+NL+PSS+SSIKA+HSN+KFA+SLGGDTVG   F
Sbjct: 79  LSFAIDYTGSSSPSPTNGKFKVFWDEKNLTPSSISSIKAKHSNVKFALSLGGDTVGDGIF 138

Query: 121 AYFKPKSVKSWVKNAFKSISRIVKEYDLDGVDIDYEHFKADPDTFTECIGQLLLRLKHHN 180
            +FKPKS+ SWV NAFKSIS IVKEYDLDGVDIDYEHFKA PDTFTECIGQLL RLK  N
Sbjct: 139 VFFKPKSITSWVNNAFKSISDIVKEYDLDGVDIDYEHFKASPDTFTECIGQLLQRLKSQN 198

Query: 181 VISFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNY 240
           +ISFASIAPF+DDTVQSHY+ALWRKYG LIDYVNFQFYSY KGTTVSQF+NHF TQSSNY
Sbjct: 199 IISFASIAPFDDDTVQSHYMALWRKYGDLIDYVNFQFYSYGKGTTVSQFLNHFDTQSSNY 258

Query: 241 QGGNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQN 300
           +GG VLASFATDGSGGL PEKGFFKAC+ LQSRGELHGIFVWSADDSKKNNFR+E QAQN
Sbjct: 259 EGGKVLASFATDGSGGLKPEKGFFKACNTLQSRGELHGIFVWSADDSKKNNFRNEIQAQN 318

Query: 301 LLANAE 305
           LLANAE
Sbjct: 319 LLANAE 324

The following BLAST results are available for this feature:

BLAST of MS020668 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146270.1	1.1e-172	99.67	chitinase 2-like [Momordica charantia]	[more]
XP_038902883.1	7.8e-147	85.67	chitinase 2-like [Benincasa hispida]	[more]
XP_022944087.1	7.3e-145	84.31	chitinase 2-like [Cucurbita moschata]	[more]
KAG6570744.1	7.3e-145	84.31	hypothetical protein SDJN03_29659, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022986910.1	9.5e-145	84.64	chitinase 2-like [Cucurbita maxima]	[more]

BLAST of MS020668 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SLP4	2.0e-105	62.03	Chitinase 1 OS=Tulipa saxatilis subsp. bakeri OX=110455 PE=1 SV=1	[more]
Q7M443	1.9e-100	63.87	Chitinase 2 OS=Tulipa saxatilis subsp. bakeri OX=110455 PE=1 SV=1	[more]
P39657	2.2e-19	31.02	RuBisCO-associated protein OS=Glycine max OX=3847 PE=2 SV=1	[more]

BLAST of MS020668 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CWU0	5.2e-173	99.67	chitinase 2-like OS=Momordica charantia OX=3673 GN=LOC111015520 PE=3 SV=1	[more]
A0A6J1FUT9	3.5e-145	84.31	chitinase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111448631 PE=3 SV=1	[more]
A0A6J1JFC6	4.6e-145	84.64	chitinase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111484508 PE=3 SV=1	[more]
A0A5D3CQZ2	7.8e-145	84.31	Chitinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G0097...	[more]
A0A1S3CLR6	7.8e-145	84.31	chitinase 2-like OS=Cucumis melo OX=3656 GN=LOC103501894 PE=3 SV=1	[more]

BLAST of MS020668 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000677	Chitinase-like	PRINTS	PR00551	2SGLOBULIN	coord: 76..91 score: 26.56 coord: 93..111 score: 36.84 coord: 263..282 score: 35.0 coord: 30..43 score: 45.24
IPR001223	Glycoside hydrolase family 18, catalytic domain	PFAM	PF00704	Glyco_hydro_18	coord: 89..226 e-value: 5.1E-16 score: 59.3
IPR001223	Glycoside hydrolase family 18, catalytic domain	PROSITE	PS51910	GH18_2	coord: 31..304 score: 26.468805
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 29..303 e-value: 1.4E-84 score: 285.9
None	No IPR available	PANTHER	PTHR46476:SF3	CHITINASE 2	coord: 7..303
None	No IPR available	PANTHER	PTHR46476	CHITINASE 2-LIKE	coord: 7..303
None	No IPR available	CDD	cd06544	GH18_narbonin	coord: 32..285 e-value: 1.10524E-127 score: 362.459
IPR001579	Glycosyl hydrolases family 18 (GH18) active site	PROSITE	PS01095	GH18_1	coord: 146..154
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 31..294

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS020668.1	MS020668.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds