MS020632 (gene) Bitter gourd (TR) v1

BLAST of MS020632 vs. NCBI nr
Match: XP_038901136.1 (subtilisin-like protease SBT1.6 [Benincasa hispida])

HSP 1 Score: 1436.8 bits (3718), Expect = 0.0e+00
Identity = 715/762 (93.83%), Postives = 736/762 (96.59%), Query Frame = 0

Query: 6   PLFFVFLLW---TVSADSTLKTFIFRVDHFLKPSVFPTHFHWYTSEFAESPKILHVYDTV 65
           P FF+ LL    TVSADS LKTFIFRVD F KPSVFPTH+HWYTSEFAESPKILHVYDTV
Sbjct: 11  PFFFLLLLTLHSTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTSEFAESPKILHVYDTV 70

Query: 66  FHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDV 125
           FHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDV
Sbjct: 71  FHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDV 130

Query: 126 IIGVFDTGISPERRSFSDVNLGPVPRRWKGACETGTKFTARNCNRKIVGARFFSKGHEAG 185
           IIGVFDTGISPERRSFSDVNLGP+PRRWKG CETGTKFTA+NCNRKIVGARFFSKGHEAG
Sbjct: 131 IIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIVGARFFSKGHEAG 190

Query: 186 ANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRYAFQASLEGYASGIAKGVAPKARL 245
           +NAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGR+AFQASLEGYASGIAKGVAPKARL
Sbjct: 191 SNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVAPKARL 250

Query: 246 AIYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAAS 305
           A+YKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAAS
Sbjct: 251 AVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAAS 310

Query: 306 RGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGRKVSGVSLYAGAP 365
           RGVFVSSSAGNDGPNGMSVTNLAPW+TTVGAGTIDRNFPAVVTLGNGRK+SGVSLYAGAP
Sbjct: 311 RGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRKISGVSLYAGAP 370

Query: 366 LNGKMYPLVYPGKSGVLSVSLCMENSLDSKLVTGKIVICDRGSNPRVAKGLVVKKAGGVG 425
           LNG MYPLVYPGKSGVLSVSLCMENSLD K V GKIVICDRGS+PRVAKGLVVKKAGGVG
Sbjct: 371 LNGTMYPLVYPGKSGVLSVSLCMENSLDPKAVAGKIVICDRGSSPRVAKGLVVKKAGGVG 430

Query: 426 MILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSANPTATIAFQGTIIGIKPAP 485
           MILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSS NP ATIAFQGTIIGIKPAP
Sbjct: 431 MILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPIATIAFQGTIIGIKPAP 490

Query: 486 VVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMA 545
           VVASFSARGPNGLNPEILKPD+IAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMA
Sbjct: 491 VVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMA 550

Query: 546 CPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRGQPMTEESTGKPSTPYDFGAGHV 605
           CPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNR QPMTEESTGK STPYDFGAGHV
Sbjct: 551 CPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKASTPYDFGAGHV 610

Query: 606 NLGLAMDPGLVYDITNTDYVNFLCSLGYGPKMIQVITRTPVSCPAKKPLPENLNYPSIAA 665
           NLGLAMDPGLVYDITNTDY+NFLCS+GYGPKMIQVITRTPV CPAKKPLPENLNYPSI +
Sbjct: 611 NLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVKCPAKKPLPENLNYPSIVS 670

Query: 666 VFSTLSKGWSTKAFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAAVKKQSYAV 725
           VFS+LSKGWSTK+FIRTVTNVGP+NS YRAKIEAPKGVTVKVKPSKLVFS  VKKQS+ V
Sbjct: 671 VFSSLSKGWSTKSFIRTVTNVGPSNSAYRAKIEAPKGVTVKVKPSKLVFSTTVKKQSFVV 730

Query: 726 TVSADSQNLALGDAGAVFGWLSWSDGKHVVRSPLVVTQIEPL 765
            +SAD+QNLALGD GAVFGWLSWSDGKHVVRSPLVVTQ+EPL
Sbjct: 731 AISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 772

BLAST of MS020632 vs. NCBI nr
Match: XP_022985703.1 (subtilisin-like protease SBT1.6 [Cucurbita maxima])

HSP 1 Score: 1429.5 bits (3699), Expect = 0.0e+00
Identity = 715/764 (93.59%), Postives = 740/764 (96.86%), Query Frame = 0

Query: 6   PLFFVFLL----WTVSADSTL-KTFIFRVDHFLKPSVFPTHFHWYTSEFAESPKILHVYD 65
           PLFF FLL     TVSADS L KTFIFRVD F KPSVFPTH+HWYTSEFAES KILHVYD
Sbjct: 7   PLFFFFLLIVHRLTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFAESHKILHVYD 66

Query: 66  TVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGS 125
           TVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGS
Sbjct: 67  TVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGS 126

Query: 126 DVIIGVFDTGISPERRSFSDVNLGPVPRRWKGACETGTKFTARNCNRKIVGARFFSKGHE 185
           DVIIGVFDTGISPERRSFSDVNLGP+PRRWKG CETGTKFTARNCNRKIVGARFFSKGHE
Sbjct: 127 DVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIVGARFFSKGHE 186

Query: 186 AGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRYAFQASLEGYASGIAKGVAPKA 245
           AG+NAAGPIIGINDTIE+RSPRDADGHGTHTASTAAGR+AFQASLEG+ASGIAKGVAPKA
Sbjct: 187 AGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFASGIAKGVAPKA 246

Query: 246 RLAIYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGA 305
           RLA+YKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGA
Sbjct: 247 RLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGA 306

Query: 306 ASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGRKVSGVSLYAG 365
           ASRG+FVSSS GNDGPNGMSVTNLAPW+TTVGAGTIDRNFPAVVTLGNGR++SGVSLYAG
Sbjct: 307 ASRGIFVSSSGGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRRISGVSLYAG 366

Query: 366 APLNGKMYPLVYPGKSGVLSVSLCMENSLDSKLVTGKIVICDRGSNPRVAKGLVVKKAGG 425
           APLN  M+PLVYPGKSGVLSVSLCM+NSLD K+V GKIVICDRGS+PRVAKGLVVKKAGG
Sbjct: 367 APLNATMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVAKGLVVKKAGG 426

Query: 426 VGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSANPTATIAFQGTIIGIKP 485
           VGMILANGISNGEGLVGDAHLLPACAVGSDEGD+MKAYASSSANPTATIAFQGTIIGIKP
Sbjct: 427 VGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSANPTATIAFQGTIIGIKP 486

Query: 486 APVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTS 545
           APVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTS
Sbjct: 487 APVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTS 546

Query: 546 MACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRGQPMTEESTGKPSTPYDFGAG 605
           MACPHVSGAAALLKSAHPDWSPAALRSAMMTTAS TDNR QPMTEESTGK STPYDFGAG
Sbjct: 547 MACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGKASTPYDFGAG 606

Query: 606 HVNLGLAMDPGLVYDITNTDYVNFLCSLGYGPKMIQVITRTPVSCPAKKPLPENLNYPSI 665
           HVNLGLAMDPGLVYDITNTDYVNFLCS+GYGPKMIQVITRTPV+CPAKKPLPENLNYPSI
Sbjct: 607 HVNLGLAMDPGLVYDITNTDYVNFLCSIGYGPKMIQVITRTPVTCPAKKPLPENLNYPSI 666

Query: 666 AAVFSTLSKGWSTKAFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAAVKKQSY 725
            AVFS+LSKGWSTK+FIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAA+KKQS+
Sbjct: 667 VAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAAMKKQSF 726

Query: 726 AVTVSADSQNLALGDAGAVFGWLSWSDGKHVVRSPLVVTQIEPL 765
            V VSAD+QNLALGD GAVFGW+SWSDGKHVVRSPLVVTQ+EPL
Sbjct: 727 VVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 770

BLAST of MS020632 vs. NCBI nr
Match: KAG7010855.1 (Subtilisin-like protease SBT1.6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1429.1 bits (3698), Expect = 0.0e+00
Identity = 713/765 (93.20%), Postives = 740/765 (96.73%), Query Frame = 0

Query: 6   PLFFVFLL-----WTVSADSTL-KTFIFRVDHFLKPSVFPTHFHWYTSEFAESPKILHVY 65
           PLFF F L     +TVSADS L KTFIFRVD F KPSVFPTH+HWYTSEFAES KILHVY
Sbjct: 7   PLFFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFAESHKILHVY 66

Query: 66  DTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYG 125
           DTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYG
Sbjct: 67  DTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYG 126

Query: 126 SDVIIGVFDTGISPERRSFSDVNLGPVPRRWKGACETGTKFTARNCNRKIVGARFFSKGH 185
           SDVIIGVFDTGISPERRSFSDVNLGP+PRRWKG CETGTKFTARNCNRKIVGARFFSKGH
Sbjct: 127 SDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIVGARFFSKGH 186

Query: 186 EAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRYAFQASLEGYASGIAKGVAPK 245
           EAG+NAAGPIIGINDTIE+RSPRDADGHGTHTASTAAGR+AFQASLEG+ASGIAKGVAPK
Sbjct: 187 EAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFASGIAKGVAPK 246

Query: 246 ARLAIYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYG 305
           ARLA+YKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYG
Sbjct: 247 ARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYG 306

Query: 306 AASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGRKVSGVSLYA 365
           AASRG+FVSSSAGNDGPNGMSVTNLAPW+TTVGAGTIDRNFPAVVTLGNGR++SGVSLYA
Sbjct: 307 AASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRRISGVSLYA 366

Query: 366 GAPLNGKMYPLVYPGKSGVLSVSLCMENSLDSKLVTGKIVICDRGSNPRVAKGLVVKKAG 425
           GAPLNG M+PLVYPGKSGVLSVSLCM+NSLD K+V GKIVICDRGS+PRVAKGLVVKKAG
Sbjct: 367 GAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVAKGLVVKKAG 426

Query: 426 GVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSANPTATIAFQGTIIGIK 485
           GVGMILANGISNGEGLVGDAHLLPACAVGSDEGD+MKAYASSS NPTATIAFQGTIIGIK
Sbjct: 427 GVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIAFQGTIIGIK 486

Query: 486 PAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGT 545
           PAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGT
Sbjct: 487 PAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGT 546

Query: 546 SMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRGQPMTEESTGKPSTPYDFGA 605
           SMACPHVSGAAALLKSAHPDWSPAALRSAMMTTAS TDNR QPMTEESTGK STPYDFGA
Sbjct: 547 SMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGKASTPYDFGA 606

Query: 606 GHVNLGLAMDPGLVYDITNTDYVNFLCSLGYGPKMIQVITRTPVSCPAKKPLPENLNYPS 665
           GHVNLGLAMDPGLVYDITNTDY+NFLCS+GYGPKMIQVITRTPV+CPAKKPLPENLNYPS
Sbjct: 607 GHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKPLPENLNYPS 666

Query: 666 IAAVFSTLSKGWSTKAFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAAVKKQS 725
           I AVFS+LSKGWSTK+FIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAA KKQS
Sbjct: 667 IVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAATKKQS 726

Query: 726 YAVTVSADSQNLALGDAGAVFGWLSWSDGKHVVRSPLVVTQIEPL 765
           + V VSAD+QNLALGD GAVFGW+SWSDGKHVVRSPLVVTQ+EPL
Sbjct: 727 FVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 771

BLAST of MS020632 vs. NCBI nr
Match: KAG6571024.1 (Subtilisin-like protease 1.6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1427.2 bits (3693), Expect = 0.0e+00
Identity = 712/766 (92.95%), Postives = 739/766 (96.48%), Query Frame = 0

Query: 6   PLFFVFLL------WTVSADSTL-KTFIFRVDHFLKPSVFPTHFHWYTSEFAESPKILHV 65
           PLFF F        +TVSADS L KTFIFRVD F KPSVFPTH+HWYTSEFAES KILHV
Sbjct: 7   PLFFFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFAESHKILHV 66

Query: 66  YDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDY 125
           YDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDY
Sbjct: 67  YDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDY 126

Query: 126 GSDVIIGVFDTGISPERRSFSDVNLGPVPRRWKGACETGTKFTARNCNRKIVGARFFSKG 185
           GSDVIIGVFDTGISPERRSFSDVNLGP+PRRWKG CETGTKFTARNCNRKIVGARFFSKG
Sbjct: 127 GSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIVGARFFSKG 186

Query: 186 HEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRYAFQASLEGYASGIAKGVAP 245
           HEAG+NAAGPIIGINDTIE+RSPRDADGHGTHTASTAAGR+AFQASLEG+ASGIAKGVAP
Sbjct: 187 HEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFASGIAKGVAP 246

Query: 246 KARLAIYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAY 305
           KARLA+YKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAY
Sbjct: 247 KARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAY 306

Query: 306 GAASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGRKVSGVSLY 365
           GAASRG+FVSSSAGNDGPNGMSVTNLAPW+TTVGAGTIDRNFPAVVTLGNGR++SGVSLY
Sbjct: 307 GAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRRISGVSLY 366

Query: 366 AGAPLNGKMYPLVYPGKSGVLSVSLCMENSLDSKLVTGKIVICDRGSNPRVAKGLVVKKA 425
           AGAPLNG M+PLVYPGKSGVLSVSLCM+NSLD K+V GKIVICDRGS+PRVAKGLVVKKA
Sbjct: 367 AGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVAKGLVVKKA 426

Query: 426 GGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSANPTATIAFQGTIIGI 485
           GGVGMILANGISNGEGLVGDAHLLPACAVGSDEGD+MKAYASSS NPTATIAFQGTIIGI
Sbjct: 427 GGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIAFQGTIIGI 486

Query: 486 KPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSG 545
           KPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSG
Sbjct: 487 KPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSG 546

Query: 546 TSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRGQPMTEESTGKPSTPYDFG 605
           TSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTAS TDNR QPMTEESTGK STPYDFG
Sbjct: 547 TSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGKASTPYDFG 606

Query: 606 AGHVNLGLAMDPGLVYDITNTDYVNFLCSLGYGPKMIQVITRTPVSCPAKKPLPENLNYP 665
           AGHVNLGLAMDPGLVYDITNTDY+NFLCS+GYGPKMIQVITRTPV+CPAKKPLPENLNYP
Sbjct: 607 AGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKPLPENLNYP 666

Query: 666 SIAAVFSTLSKGWSTKAFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAAVKKQ 725
           SI AVFS+LSKGWSTK+FIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAA KKQ
Sbjct: 667 SIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAATKKQ 726

Query: 726 SYAVTVSADSQNLALGDAGAVFGWLSWSDGKHVVRSPLVVTQIEPL 765
           S+ V VSAD+QNLALGD GAVFGW+SWSDGKHVVRSPLVVTQ+EPL
Sbjct: 727 SFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 772

BLAST of MS020632 vs. NCBI nr
Match: XP_022943985.1 (subtilisin-like protease SBT1.6 [Cucurbita moschata])

HSP 1 Score: 1426.8 bits (3692), Expect = 0.0e+00
Identity = 712/762 (93.44%), Postives = 739/762 (96.98%), Query Frame = 0

Query: 8   FFVFLL----WTVSADSTL-KTFIFRVDHFLKPSVFPTHFHWYTSEFAESPKILHVYDTV 67
           FF FLL    +TVSADS L KTFIFRVD F KPSVFPTH+HWYTSEFAES KILHVYDTV
Sbjct: 13  FFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFAESHKILHVYDTV 72

Query: 68  FHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDV 127
           FHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDV
Sbjct: 73  FHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDV 132

Query: 128 IIGVFDTGISPERRSFSDVNLGPVPRRWKGACETGTKFTARNCNRKIVGARFFSKGHEAG 187
           IIGVFDTGISPERRSFSDVNLGP+PRRWKG CETGTKFTARNCNRKIVGARFFSKGHEAG
Sbjct: 133 IIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIVGARFFSKGHEAG 192

Query: 188 ANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRYAFQASLEGYASGIAKGVAPKARL 247
           +NAAGPIIGINDTIE+RSPRDADGHGTHTASTAAGR+AFQASLEG+ASGIAKGVAPKARL
Sbjct: 193 SNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFASGIAKGVAPKARL 252

Query: 248 AIYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAAS 307
           A+YKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAAS
Sbjct: 253 AVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAAS 312

Query: 308 RGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGRKVSGVSLYAGAP 367
           RG+FVSSSAGNDGPNGMSVTNLAPW+TTVGAGTIDRNFPAVVTLGNGR++SGVSLYAGAP
Sbjct: 313 RGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRRISGVSLYAGAP 372

Query: 368 LNGKMYPLVYPGKSGVLSVSLCMENSLDSKLVTGKIVICDRGSNPRVAKGLVVKKAGGVG 427
           LNG M+PLVYPGKSGVLSVSLCM+NSLD K+V GKIVICDRGS+PRVAKGLVVKKAGGVG
Sbjct: 373 LNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVAKGLVVKKAGGVG 432

Query: 428 MILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSANPTATIAFQGTIIGIKPAP 487
           MILANGISNGEGLVGDAHLLPACAVGSDEGD+MKAYASSS NPTATIAFQGTIIGIKPAP
Sbjct: 433 MILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIAFQGTIIGIKPAP 492

Query: 488 VVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMA 547
           VVASFSARGPNGLNPEILKPD+IAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMA
Sbjct: 493 VVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMA 552

Query: 548 CPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRGQPMTEESTGKPSTPYDFGAGHV 607
           CPHVSGAAALLKSAHPDWSPAALRSAMMTTAS TDNR QPMTEESTGK STPYDFGAGHV
Sbjct: 553 CPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGKASTPYDFGAGHV 612

Query: 608 NLGLAMDPGLVYDITNTDYVNFLCSLGYGPKMIQVITRTPVSCPAKKPLPENLNYPSIAA 667
           NLGLAMDPGLVYDITNTDY+NFLCS+GYGPKMIQVITRTPV+CPAKKPLPENLNYPSI A
Sbjct: 613 NLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKPLPENLNYPSIVA 672

Query: 668 VFSTLSKGWSTKAFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAAVKKQSYAV 727
           VFS+LSKGWSTK+FIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAA KKQS+ V
Sbjct: 673 VFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAATKKQSFVV 732

Query: 728 TVSADSQNLALGDAGAVFGWLSWSDGKHVVRSPLVVTQIEPL 765
            VSAD+QNLALGD GAVFGW+SWSDGKHVVRSPLVVTQ+EPL
Sbjct: 733 AVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 774

BLAST of MS020632 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 1156.7 bits (2991), Expect = 0.0e+00
Identity = 565/766 (73.76%), Postives = 662/766 (86.42%), Query Frame = 0

Query: 1   SAISLPLFFVFLLWTVSADSTLKTFIFRVDHFLKPSVFPTHFHWYTSEFAESPKILHVYD 60
           S I L LF  F   + +A    KTFIFR+D    PS+FPTH+HWY++EFAE  +I+HVY 
Sbjct: 4   STIVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEESRIVHVYH 63

Query: 61  TVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGS 120
           TVFHGFSA +T  + D++  HP+VLAVFEDRRR+LHTTRSPQFLGL+NQ+GLWS+SDYGS
Sbjct: 64  TVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSESDYGS 123

Query: 121 DVIIGVFDTGISPERRSFSDVNLGPVPRRWKGACETGTKFTARNCNRKIVGARFFSKGHE 180
           DVIIGVFDTGI PERRSFSD+NLGP+P+RW+G CE+G +F+ RNCNRKI+GARFF+KG +
Sbjct: 124 DVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQ 183

Query: 181 AGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRYAFQASLEGYASGIAKGVAPKA 240
           A       I GIN T+E+ SPRDADGHGTHT+STAAGR+AF+AS+ GYASG+AKGVAPKA
Sbjct: 184 AAV-----IGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKA 243

Query: 241 RLAIYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGA 300
           R+A YKVCWK+SGC DSDILAAFDAAV DGVDVISISIGGGDG++SPYYLDPIAIG+YGA
Sbjct: 244 RIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGA 303

Query: 301 ASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGRKVSGVSLYAG 360
           AS+G+FVSSSAGN+GPNGMSVTNLAPW+TTVGA TIDRNFPA   LG+G ++ GVSLYAG
Sbjct: 304 ASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAG 363

Query: 361 APLNGKMYPLVYPGKSGVLSVSLCMENSLDSKLVTGKIVICDRGSNPRVAKGLVVKKAGG 420
            PLNG+M+P+VYPGKSG+ S SLCMEN+LD K V GKIVICDRGS+PRVAKGLVVKKAGG
Sbjct: 364 VPLNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGG 423

Query: 421 VGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSANPTATIAFQGTIIGIKP 480
           VGMILANG SNGEGLVGDAHL+PACAVGS+EGD +KAYASS  NP A+I F+GTI+GIKP
Sbjct: 424 VGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKP 483

Query: 481 APVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTS 540
           APV+ASFS RGPNGL+PEILKPDLIAPGVNILAAWTDAVGPTGL  D RKTEFNILSGTS
Sbjct: 484 APVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTS 543

Query: 541 MACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRGQPMTEESTGKPSTPYDFGAG 600
           MACPHVSGAAALLKSAHPDWSPA +RSAMMTT ++ DN  + + +ESTGK +TPYD+G+G
Sbjct: 544 MACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGSG 603

Query: 601 HVNLGLAMDPGLVYDITNTDYVNFLCSLGYGPKMIQVITRTPVSCP-AKKPLPENLNYPS 660
           H+NLG AM+PGLVYDITN DY+ FLCS+GYGPK IQVITRTPV CP  +KP P NLNYPS
Sbjct: 604 HLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPS 663

Query: 661 IAAVFSTLSKGWSTKAFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAAVKKQS 720
           I AVF T  +G  +K  IRT TNVG A +VYRA+IE+P+GVTV VKP +LVF++AVK++S
Sbjct: 664 ITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRS 723

Query: 721 YAVTVSADSQNLALGDAGAVFGWLSWSD-GKHVVRSPLVVTQIEPL 765
           YAVTV+ +++N+ LG+ GAVFG ++W D GKHVVRSP+VVTQ++ L
Sbjct: 724 YAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVTQMDTL 764

BLAST of MS020632 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 858.2 bits (2216), Expect = 7.0e-248
Identity = 455/781 (58.26%), Postives = 550/781 (70.42%), Query Frame = 0

Query: 8   FFVFLLWTVSADSTLK------TFIFRVDHFLKPSVFPTHFHWYTSEFA----ESPKILH 67
           F+ F L T+S+ S+        T+I  VDH  KPS+FPTHFHWYTS  A      P I+H
Sbjct: 5   FYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSIIH 64

Query: 68  VYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLR--NQRGLWSD 127
            YDTVFHGFSA LT Q    +  HP V++V  ++ R LHTTRSP+FLGLR  ++ GL  +
Sbjct: 65  TYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEE 124

Query: 128 SDYGSDVIIGVFDTGISPERRSFSDVNLGPVPRRWKGACETGTKFTARNCNRKIVGARFF 187
           SD+GSD++IGV DTG+ PER SF D  LGPVP +WKG C     F    CNRK+VGARFF
Sbjct: 125 SDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFF 184

Query: 188 SKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRYAFQASLEGYASGIAKG 247
             G+EA          +N+T E+RSPRD+DGHGTHTAS +AGRY F AS  GYA G+A G
Sbjct: 185 CGGYEATNGK------MNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAG 244

Query: 248 VAPKARLAIYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAI 307
           +APKARLA YKVCW NSGC+DSDILAAFD AV DGVDVIS+S+G   GV  PYYLD IAI
Sbjct: 245 MAPKARLAAYKVCW-NSGCYDSDILAAFDTAVADGVDVISLSVG---GVVVPYYLDAIAI 304

Query: 308 GAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGRKVSGV 367
           GA+GA  RG+FVS+SAGN GP  ++VTN+APW+TTVGAGTIDR+FPA V LGNG+ +SGV
Sbjct: 305 GAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGV 364

Query: 368 SLYAGAPLN-GKMYPLVYPGK---SGVLSVSLCMENSLDSKLVTGKIVICDRGSNPRVAK 427
           S+Y G  L+ G+MYPLVY G        S SLC+E SLD  LV GKIV+CDRG N R  K
Sbjct: 365 SVYGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATK 424

Query: 428 GLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYAS------SSANP 487
           G +V+K GG+GMI+ANG+ +GEGLV D H+LPA +VG+  GD ++ Y S      SS +P
Sbjct: 425 GEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHP 484

Query: 488 TATIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLD 547
           TATI F+GT +GI+PAPVVASFSARGPN   PEILKPD+IAPG+NILAAW D +GP+G+ 
Sbjct: 485 TATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVT 544

Query: 548 FDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRGQPMTE 607
            D R+TEFNILSGTSMACPHVSG AALLK+AHPDWSPAA+RSA++TTA   DN G+PM +
Sbjct: 545 SDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMD 604

Query: 608 ESTGKPSTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSLGYGPKMIQVITRTPVSC 667
           ESTG  S+  D+G+GHV+   AMDPGLVYDIT+ DY+NFLC+  Y    I  ITR    C
Sbjct: 605 ESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADC 664

Query: 668 PAKKPLPE--NLNYPSIAAVFSTLSKGWSTKAFIRTVTNVGPANSVYRAKIEAPKGVTVK 727
              +      NLNYPS + VF    +   +  FIRTVTNVG ++SVY  KI  P+G TV 
Sbjct: 665 DGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVT 724

Query: 728 VKPSKLVFSAAVKKQSYAVTVSADSQNLALGDAGAVFGWLSWSDGKHVVRSPLVVTQIEP 765
           V+P KL F    +K S+ V V      L+ G      G + WSDGK  V SPLVVT  +P
Sbjct: 725 VEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQQP 775

BLAST of MS020632 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 741.9 bits (1914), Expect = 7.3e-213
Identity = 399/770 (51.82%), Postives = 526/770 (68.31%), Query Frame = 0

Query: 1   SAISLPLFFVFL----LWTVSADSTLKTFIFRVDHFLKPSVFPTHFHWYTS---EFAESP 60
           S +S   FF+ L        S+ S   T+I  +     PS F  H +WY S     ++S 
Sbjct: 4   SFLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSA 63

Query: 61  KILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGL-RNQRGL 120
           ++L+ Y+   HGFS  LTQ++ DS+   P V++V  + R +LHTTR+P FLGL  +   L
Sbjct: 64  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 123

Query: 121 WSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPVPRRWKGACETGTKFTARNCNRKIVGA 180
           + ++   SDV++GV DTG+ PE +S+SD   GP+P  WKG CE GT FTA  CNRK++GA
Sbjct: 124 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 183

Query: 181 RFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRYAFQASLEGYASGI 240
           RFF++G+E   +  GP   I+++ E RSPRD DGHGTHT+STAAG     ASL GYASG 
Sbjct: 184 RFFARGYE---STMGP---IDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGT 243

Query: 241 AKGVAPKARLAIYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDP 300
           A+G+AP+AR+A+YKVCW   GCF SDILAA D A+ D V+V+S+S+GGG    S YY D 
Sbjct: 244 ARGMAPRARVAVYKVCWL-GGCFSSDILAAIDKAIADNVNVLSMSLGGG---MSDYYRDG 303

Query: 301 IAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGRKV 360
           +AIGA+ A  RG+ VS SAGN GP+  S++N+APWITTVGAGT+DR+FPA+  LGNG+  
Sbjct: 304 VAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNF 363

Query: 361 SGVSLYAGAPLNGKMYPLVYPGK-SGVLSVSLCMENSLDSKLVTGKIVICDRGSNPRVAK 420
           +GVSL+ G  L  K+ P +Y G  S   + +LCM  +L  + V GKIV+CDRG N RV K
Sbjct: 364 TGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQK 423

Query: 421 GLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSANPTATIAF 480
           G VVK AGGVGMILAN  +NGE LV DAHLLPA  VG   GD ++ Y ++  NPTA+I+ 
Sbjct: 424 GDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISI 483

Query: 481 QGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKT 540
            GT++G+KP+PVVA+FS+RGPN + P ILKPDLIAPGVNILAAWT A GPTGL  D+R+ 
Sbjct: 484 LGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRV 543

Query: 541 EFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRGQPMTEESTGKP 600
           EFNI+SGTSM+CPHVSG AALLKS HP+WSPAA+RSA+MTTA  T   G+P+ + +TGKP
Sbjct: 544 EFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKP 603

Query: 601 STPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSLGYGPKMIQVITRTPVSC-PAKKP 660
           STP+D GAGHV+   A +PGL+YD+T  DY+ FLC+L Y    I+ ++R   +C P+K  
Sbjct: 604 STPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSY 663

Query: 661 LPENLNYPSIAAVFSTLSKGWSTKAFIRTVTNVGPANSVYRAKIEA-PKGVTVKVKPSKL 720
              +LNYPS    F+    G     + RTVT+VG A + Y  K+ +   GV + V+P+ L
Sbjct: 664 SVADLNYPS----FAVNVDGVGAYKYTRTVTSVGGAGT-YSVKVTSETTGVKISVEPAVL 723

Query: 721 VFSAAVKKQSYAVTVSADSQNLALGDAGAVFGWLSWSDGKHVVRSPLVVT 760
            F  A +K+SY VT + DS   +  ++   FG + WSDGKHVV SP+ ++
Sbjct: 724 NFKEANEKKSYTVTFTVDSSKPSGSNS---FGSIEWSDGKHVVGSPVAIS 755

BLAST of MS020632 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 736.1 bits (1899), Expect = 4.0e-211
Identity = 386/758 (50.92%), Postives = 516/758 (68.07%), Query Frame = 0

Query: 1   SAISLPLFFVFLLWTVSADSTLKTFIFRVDHFLKPSVFPTHFHWYTSEFAESPKILHVYD 60
           S+I++   F+FLL   +A    KT+I RV+H  KP  F TH  WYTS+      +L+ Y 
Sbjct: 9   SSITIITTFLFLLLHTTAK---KTYIIRVNHSDKPESFLTHHDWYTSQLNSESSLLYTYT 68

Query: 61  TVFHGFSATLTQQQVDS-IGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYG 120
           T FHGFSA L   + DS +    S+L +FED    LHTTR+P+FLGL ++ G+       
Sbjct: 69  TSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSS 128

Query: 121 SDVIIGVFDTGISPERRSFSDVNLGPVPRRWKGACETGTKFTARNCNRKIVGARFFSKGH 180
           + VIIGV DTG+ PE RSF D ++  +P +WKG CE+G+ F ++ CN+K++GAR FSKG 
Sbjct: 129 NGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGF 188

Query: 181 EAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRYAFQASLEGYASGIAKGVAPK 240
           +  +       G +   E  SPRD DGHGTHT++TAAG     AS  GYA+G A+G+A +
Sbjct: 189 QMASGG-----GFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATR 248

Query: 241 ARLAIYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYG 300
           AR+A YKVCW ++GCF SDILAA D A+ DGVDV+S+S+GGG   S+PYY D IAIGA+ 
Sbjct: 249 ARVATYKVCW-STGCFGSDILAAMDRAILDGVDVLSLSLGGG---SAPYYRDTIAIGAFS 308

Query: 301 AASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGRKVSGVSLYA 360
           A  RGVFVS SAGN GP   SV N+APW+ TVGAGT+DR+FPA   LGNG++++GVSLY+
Sbjct: 309 AMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYS 368

Query: 361 GAPLNGKMYPLVYPGKSGVLSVSLCMENSLDSKLVTGKIVICDRGSNPRVAKGLVVKKAG 420
           G  +  K   LVY  K    S +LC+  SLDS +V GKIV+CDRG N RV KG VV+ AG
Sbjct: 369 GVGMGTKPLELVY-NKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAG 428

Query: 421 GVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSANPTATIAFQGTIIGIK 480
           G+GMI+AN  ++GE LV D+HLLPA AVG   GD ++ Y  S + PTA + F+GT++ +K
Sbjct: 429 GLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVK 488

Query: 481 PAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGT 540
           P+PVVA+FS+RGPN + PEILKPD+I PGVNILA W+DA+GPTGLD D+R+T+FNI+SGT
Sbjct: 489 PSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGT 548

Query: 541 SMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRGQPMTEESTGKPSTPYDFGA 600
           SM+CPH+SG A LLK+AHP+WSP+A++SA+MTTA + DN   P+ + +    S PY  G+
Sbjct: 549 SMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGS 608

Query: 601 GHVNLGLAMDPGLVYDITNTDYVNFLCSLGYG-PKMIQVITRTPVSCPAKKPLPENLNYP 660
           GHV+   A+ PGLVYDI+  +Y+ FLCSL Y    ++ ++ R  V+C  K   P  LNYP
Sbjct: 609 GHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYP 668

Query: 661 SIAAVFSTLSKGWSTKAFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAAVKKQ 720
           S    FS L  G     + R VTNVG A+SVY+  +     V + VKPSKL F +  +K+
Sbjct: 669 S----FSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKK 728

Query: 721 SYAVTVSADSQNLALGDAGAVFGWLSWSDGKHVVRSPL 757
            Y VT     + +++ +  A FG ++WS+ +H VRSP+
Sbjct: 729 RYTVTF-VSKKGVSMTNK-AEFGSITWSNPQHEVRSPV 747

BLAST of MS020632 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 703.4 bits (1814), Expect = 2.9e-201
Identity = 393/787 (49.94%), Postives = 507/787 (64.42%), Query Frame = 0

Query: 3   ISLPLFFVFL-LWTVSADSTLKTFIFRVDHFLKPSVFPTHFHWYTS------------EF 62
           I L +  +FL   T +  ST KT++  +D    P  +  H  WY+S            E 
Sbjct: 14  IILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEE 73

Query: 63  AESPKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQ 122
             + +IL+ Y T FHG +A LTQ++ + + +   V+AV  + R +LHTTRSP FLGL  Q
Sbjct: 74  GNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQ 133

Query: 123 RG--LWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPVPRRWKGACETGTKFTARNCNR 182
               +W++     DV++GV DTGI PE  SF+D  + PVP  W+GACETG +F  RNCNR
Sbjct: 134 ESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNR 193

Query: 183 KIVGARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRYAFQASLEG 242
           KIVGAR F +G+EA          I++ +EY+SPRD DGHGTHTA+T AG     A+L G
Sbjct: 194 KIVGARVFYRGYEAATGK------IDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFG 253

Query: 243 YASGIAKGVAPKARLAIYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSP 302
           +A G A+G+A KAR+A YKVCW   GCF SDIL+A D AV DGV V+SIS+GGG    S 
Sbjct: 254 FAYGTARGMAQKARVAAYKVCWV-GGCFSSDILSAVDQAVADGVQVLSISLGGG---VST 313

Query: 303 YYLDPIAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLG 362
           Y  D ++I  +GA   GVFVS SAGN GP+ +S+TN++PWITTVGA T+DR+FPA V +G
Sbjct: 314 YSRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIG 373

Query: 363 NGRKVSGVSLYAGAPL--NGKMYPLVYPGK--SGVLSVSLCMENSLDSKLVTGKIVICDR 422
             R   GVSLY G  +    K YPLVY G+  S     S C++ +LD + V GKIVICDR
Sbjct: 374 TMRTFKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDR 433

Query: 423 GSNPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSA 482
           G  PRV KG VVK+AGG+GM+L N  +NGE LV D+H+LPA AVG  EG  +K YA +S 
Sbjct: 434 GVTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSK 493

Query: 483 NPTATIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTG 542
             TA++   GT IGIKP+PVVA+FS+RGPN L+ EILKPDL+APGVNILAAWT  + P+ 
Sbjct: 494 KATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSS 553

Query: 543 LDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRGQPM 602
           L  D R+ +FNILSGTSM+CPHVSG AAL+KS HPDWSPAA++SA+MTTA + DN  +P+
Sbjct: 554 LSSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPL 613

Query: 603 TEESTGKPSTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSLGYGPKMIQVIT---- 662
           T+ S   PS+PYD GAGH++   A DPGLVYDI   +Y  FLC+    P  ++V T    
Sbjct: 614 TDASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSN 673

Query: 663 RTPVSCPAKKPLPENLNYPSIAAVF--STLSKGWSTKAFIRTVTNVGPANSVYRAKIEAP 722
           RT     AK   P NLNYP+I+A+F  +T  K  + +   RTVTNVGP  S Y+  +   
Sbjct: 674 RTCKHTLAKN--PGNLNYPAISALFPENTHVKAMTLR---RTVTNVGPHISSYKVSVSPF 733

Query: 723 KGVTVKVKPSKLVFSAAVKKQSYAVTVSADSQNLALGDAGAVFGWLSWSDGKHVVRSPLV 765
           KG +V V+P  L F++  +K SY VT     +          FG L W    H VRSP++
Sbjct: 734 KGASVTVQPKTLNFTSKHQKLSYTVTFRTRFRM-----KRPEFGGLVWKSTTHKVRSPVI 780

BLAST of MS020632 vs. ExPASy TrEMBL
Match: A0A6J1JEF5 (subtilisin-like protease SBT1.6 OS=Cucurbita maxima OX=3661 GN=LOC111483679 PE=3 SV=1)

HSP 1 Score: 1429.5 bits (3699), Expect = 0.0e+00
Identity = 715/764 (93.59%), Postives = 740/764 (96.86%), Query Frame = 0

Query: 6   PLFFVFLL----WTVSADSTL-KTFIFRVDHFLKPSVFPTHFHWYTSEFAESPKILHVYD 65
           PLFF FLL     TVSADS L KTFIFRVD F KPSVFPTH+HWYTSEFAES KILHVYD
Sbjct: 7   PLFFFFLLIVHRLTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFAESHKILHVYD 66

Query: 66  TVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGS 125
           TVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGS
Sbjct: 67  TVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGS 126

Query: 126 DVIIGVFDTGISPERRSFSDVNLGPVPRRWKGACETGTKFTARNCNRKIVGARFFSKGHE 185
           DVIIGVFDTGISPERRSFSDVNLGP+PRRWKG CETGTKFTARNCNRKIVGARFFSKGHE
Sbjct: 127 DVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIVGARFFSKGHE 186

Query: 186 AGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRYAFQASLEGYASGIAKGVAPKA 245
           AG+NAAGPIIGINDTIE+RSPRDADGHGTHTASTAAGR+AFQASLEG+ASGIAKGVAPKA
Sbjct: 187 AGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFASGIAKGVAPKA 246

Query: 246 RLAIYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGA 305
           RLA+YKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGA
Sbjct: 247 RLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGA 306

Query: 306 ASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGRKVSGVSLYAG 365
           ASRG+FVSSS GNDGPNGMSVTNLAPW+TTVGAGTIDRNFPAVVTLGNGR++SGVSLYAG
Sbjct: 307 ASRGIFVSSSGGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRRISGVSLYAG 366

Query: 366 APLNGKMYPLVYPGKSGVLSVSLCMENSLDSKLVTGKIVICDRGSNPRVAKGLVVKKAGG 425
           APLN  M+PLVYPGKSGVLSVSLCM+NSLD K+V GKIVICDRGS+PRVAKGLVVKKAGG
Sbjct: 367 APLNATMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVAKGLVVKKAGG 426

Query: 426 VGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSANPTATIAFQGTIIGIKP 485
           VGMILANGISNGEGLVGDAHLLPACAVGSDEGD+MKAYASSSANPTATIAFQGTIIGIKP
Sbjct: 427 VGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSANPTATIAFQGTIIGIKP 486

Query: 486 APVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTS 545
           APVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTS
Sbjct: 487 APVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTS 546

Query: 546 MACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRGQPMTEESTGKPSTPYDFGAG 605
           MACPHVSGAAALLKSAHPDWSPAALRSAMMTTAS TDNR QPMTEESTGK STPYDFGAG
Sbjct: 547 MACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGKASTPYDFGAG 606

Query: 606 HVNLGLAMDPGLVYDITNTDYVNFLCSLGYGPKMIQVITRTPVSCPAKKPLPENLNYPSI 665
           HVNLGLAMDPGLVYDITNTDYVNFLCS+GYGPKMIQVITRTPV+CPAKKPLPENLNYPSI
Sbjct: 607 HVNLGLAMDPGLVYDITNTDYVNFLCSIGYGPKMIQVITRTPVTCPAKKPLPENLNYPSI 666

Query: 666 AAVFSTLSKGWSTKAFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAAVKKQSY 725
            AVFS+LSKGWSTK+FIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAA+KKQS+
Sbjct: 667 VAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAAMKKQSF 726

Query: 726 AVTVSADSQNLALGDAGAVFGWLSWSDGKHVVRSPLVVTQIEPL 765
            V VSAD+QNLALGD GAVFGW+SWSDGKHVVRSPLVVTQ+EPL
Sbjct: 727 VVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 770

BLAST of MS020632 vs. ExPASy TrEMBL
Match: A0A6J1FYE5 (subtilisin-like protease SBT1.6 OS=Cucurbita moschata OX=3662 GN=LOC111448551 PE=3 SV=1)

HSP 1 Score: 1426.8 bits (3692), Expect = 0.0e+00
Identity = 712/762 (93.44%), Postives = 739/762 (96.98%), Query Frame = 0

Query: 8   FFVFLL----WTVSADSTL-KTFIFRVDHFLKPSVFPTHFHWYTSEFAESPKILHVYDTV 67
           FF FLL    +TVSADS L KTFIFRVD F KPSVFPTH+HWYTSEFAES KILHVYDTV
Sbjct: 13  FFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFAESHKILHVYDTV 72

Query: 68  FHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDV 127
           FHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDV
Sbjct: 73  FHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDV 132

Query: 128 IIGVFDTGISPERRSFSDVNLGPVPRRWKGACETGTKFTARNCNRKIVGARFFSKGHEAG 187
           IIGVFDTGISPERRSFSDVNLGP+PRRWKG CETGTKFTARNCNRKIVGARFFSKGHEAG
Sbjct: 133 IIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIVGARFFSKGHEAG 192

Query: 188 ANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRYAFQASLEGYASGIAKGVAPKARL 247
           +NAAGPIIGINDTIE+RSPRDADGHGTHTASTAAGR+AFQASLEG+ASGIAKGVAPKARL
Sbjct: 193 SNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFASGIAKGVAPKARL 252

Query: 248 AIYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAAS 307
           A+YKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAAS
Sbjct: 253 AVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAAS 312

Query: 308 RGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGRKVSGVSLYAGAP 367
           RG+FVSSSAGNDGPNGMSVTNLAPW+TTVGAGTIDRNFPAVVTLGNGR++SGVSLYAGAP
Sbjct: 313 RGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRRISGVSLYAGAP 372

Query: 368 LNGKMYPLVYPGKSGVLSVSLCMENSLDSKLVTGKIVICDRGSNPRVAKGLVVKKAGGVG 427
           LNG M+PLVYPGKSGVLSVSLCM+NSLD K+V GKIVICDRGS+PRVAKGLVVKKAGGVG
Sbjct: 373 LNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVAKGLVVKKAGGVG 432

Query: 428 MILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSANPTATIAFQGTIIGIKPAP 487
           MILANGISNGEGLVGDAHLLPACAVGSDEGD+MKAYASSS NPTATIAFQGTIIGIKPAP
Sbjct: 433 MILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIAFQGTIIGIKPAP 492

Query: 488 VVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMA 547
           VVASFSARGPNGLNPEILKPD+IAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMA
Sbjct: 493 VVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMA 552

Query: 548 CPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRGQPMTEESTGKPSTPYDFGAGHV 607
           CPHVSGAAALLKSAHPDWSPAALRSAMMTTAS TDNR QPMTEESTGK STPYDFGAGHV
Sbjct: 553 CPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGKASTPYDFGAGHV 612

Query: 608 NLGLAMDPGLVYDITNTDYVNFLCSLGYGPKMIQVITRTPVSCPAKKPLPENLNYPSIAA 667
           NLGLAMDPGLVYDITNTDY+NFLCS+GYGPKMIQVITRTPV+CPAKKPLPENLNYPSI A
Sbjct: 613 NLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKPLPENLNYPSIVA 672

Query: 668 VFSTLSKGWSTKAFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAAVKKQSYAV 727
           VFS+LSKGWSTK+FIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAA KKQS+ V
Sbjct: 673 VFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAATKKQSFVV 732

Query: 728 TVSADSQNLALGDAGAVFGWLSWSDGKHVVRSPLVVTQIEPL 765
            VSAD+QNLALGD GAVFGW+SWSDGKHVVRSPLVVTQ+EPL
Sbjct: 733 AVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 774

BLAST of MS020632 vs. ExPASy TrEMBL
Match: A0A1S3C7D0 (subtilisin-like protease SBT1.6 OS=Cucumis melo OX=3656 GN=LOC103497323 PE=3 SV=1)

HSP 1 Score: 1422.5 bits (3681), Expect = 0.0e+00
Identity = 705/761 (92.64%), Postives = 733/761 (96.32%), Query Frame = 0

Query: 6   PLFFVFLLW--TVSADSTLKTFIFRVDHFLKPSVFPTHFHWYTSEFAESPKILHVYDTVF 65
           P FF FLL   TVS+ S LKTFI R+D F KPSVFPTH+HWYTSEF +SP+ILHVYDTVF
Sbjct: 11  PFFFSFLLLLSTVSSLSPLKTFIVRIDRFSKPSVFPTHYHWYTSEFTQSPQILHVYDTVF 70

Query: 66  HGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVI 125
           HGFSATLTQ+QVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVI
Sbjct: 71  HGFSATLTQEQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVI 130

Query: 126 IGVFDTGISPERRSFSDVNLGPVPRRWKGACETGTKFTARNCNRKIVGARFFSKGHEAGA 185
           IGVFDTGISPERRSFSDVNLGP+PRRWKG CETGTKFTA+NCNRKIVGARFFSKGHEAGA
Sbjct: 131 IGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIVGARFFSKGHEAGA 190

Query: 186 NAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRYAFQASLEGYASGIAKGVAPKARLA 245
           NAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGR++FQASLEGYASGIAKGVAPKARLA
Sbjct: 191 NAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGYASGIAKGVAPKARLA 250

Query: 246 IYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAASR 305
           +YKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAAS+
Sbjct: 251 VYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAASK 310

Query: 306 GVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGRKVSGVSLYAGAPL 365
           GVFVSSSAGNDGPNGMSVTNLAPW+TTVGAGTIDRNFP+VVTLGNGRK+ GVSLYAGAPL
Sbjct: 311 GVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPSVVTLGNGRKIYGVSLYAGAPL 370

Query: 366 NGKMYPLVYPGKSGVLSVSLCMENSLDSKLVTGKIVICDRGSNPRVAKGLVVKKAGGVGM 425
           NG MYPLVYPGKSGVLSVSLCMENSLD K+VTGKIVICDRGS+PRVAKGLVVKKAGGVGM
Sbjct: 371 NGTMYPLVYPGKSGVLSVSLCMENSLDPKVVTGKIVICDRGSSPRVAKGLVVKKAGGVGM 430

Query: 426 ILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSANPTATIAFQGTIIGIKPAPV 485
           ILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSS NPTATIAFQGTIIGIKPAPV
Sbjct: 431 ILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTATIAFQGTIIGIKPAPV 490

Query: 486 VASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMAC 545
           VASFSARGPNGL PEILKPD+IAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMAC
Sbjct: 491 VASFSARGPNGLTPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMAC 550

Query: 546 PHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRGQPMTEESTGKPSTPYDFGAGHVN 605
           PHVSGAAALLKSAHPDWSPAALRSAMMTTASI DNR QPMTEESTGKPSTPYDFGAGHVN
Sbjct: 551 PHVSGAAALLKSAHPDWSPAALRSAMMTTASIIDNRRQPMTEESTGKPSTPYDFGAGHVN 610

Query: 606 LGLAMDPGLVYDITNTDYVNFLCSLGYGPKMIQVITRTPVSCPAKKPLPENLNYPSIAAV 665
           LGLAMDPGL+YDITNTDY+NFLCS+GYGPKMIQVITRTPV CP KKPLPENLNYPSI AV
Sbjct: 611 LGLAMDPGLIYDITNTDYINFLCSIGYGPKMIQVITRTPVRCPTKKPLPENLNYPSIVAV 670

Query: 666 FSTLSKGWSTKAFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAAVKKQSYAVT 725
           FS+LSKGWSTK+FIRTVTNVGP+NSVYR KIEAPKGVTVKVKPSKLVFS  VKKQS+ V 
Sbjct: 671 FSSLSKGWSTKSFIRTVTNVGPSNSVYRVKIEAPKGVTVKVKPSKLVFSTTVKKQSFVVA 730

Query: 726 VSADSQNLALGDAGAVFGWLSWSDGKHVVRSPLVVTQIEPL 765
           +SAD+QNLALGD GAVFGWLSWSDGKHVVRSPLVVTQ+EPL
Sbjct: 731 ISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 771

BLAST of MS020632 vs. ExPASy TrEMBL
Match: A0A0A0LJ58 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G270180 PE=3 SV=1)

HSP 1 Score: 1420.2 bits (3675), Expect = 0.0e+00
Identity = 700/756 (92.59%), Postives = 729/756 (96.43%), Query Frame = 0

Query: 9   FVFLLWTVSADSTLKTFIFRVDHFLKPSVFPTHFHWYTSEFAESPKILHVYDTVFHGFSA 68
           F+ LL TVS+ S LKTFI R+D F KPSVFPTH+HWYTSEF +SP+ILHVYDTVFHGFSA
Sbjct: 16  FLLLLSTVSSHSPLKTFIVRIDRFSKPSVFPTHYHWYTSEFTQSPQILHVYDTVFHGFSA 75

Query: 69  TLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFD 128
           TLTQ QVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFD
Sbjct: 76  TLTQDQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFD 135

Query: 129 TGISPERRSFSDVNLGPVPRRWKGACETGTKFTARNCNRKIVGARFFSKGHEAGANAAGP 188
           TGISPERRSFSDVNLGP+PRRWKG CETGTKFTA+NCNRKIVGARFFSKGHEAGANAAGP
Sbjct: 136 TGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIVGARFFSKGHEAGANAAGP 195

Query: 189 IIGINDTIEYRSPRDADGHGTHTASTAAGRYAFQASLEGYASGIAKGVAPKARLAIYKVC 248
           IIGINDTIEYRSPRDADGHGTHTASTAAGR++FQASLEGYASGIAKGVAPKARLA+YKVC
Sbjct: 196 IIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGYASGIAKGVAPKARLAVYKVC 255

Query: 249 WKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAASRGVFVS 308
           WKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIG+YGAAS+GVFVS
Sbjct: 256 WKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGSYGAASKGVFVS 315

Query: 309 SSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGRKVSGVSLYAGAPLNGKMY 368
           SSAGNDGPNGMSVTNLAPW+TTVGAGTIDRNFP+VVTLGNGRK+ GVSLYAGAPLNG MY
Sbjct: 316 SSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPSVVTLGNGRKIYGVSLYAGAPLNGTMY 375

Query: 369 PLVYPGKSGVLSVSLCMENSLDSKLVTGKIVICDRGSNPRVAKGLVVKKAGGVGMILANG 428
           PLVYPGKSGVLSVSLCMENSLD K+VTGKIVICDRGS+PRVAKGLVVKKAGGVGMILANG
Sbjct: 376 PLVYPGKSGVLSVSLCMENSLDPKVVTGKIVICDRGSSPRVAKGLVVKKAGGVGMILANG 435

Query: 429 ISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSANPTATIAFQGTIIGIKPAPVVASFS 488
           ISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSS NPTATIAFQGTIIGIKPAPVVASFS
Sbjct: 436 ISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTATIAFQGTIIGIKPAPVVASFS 495

Query: 489 ARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMACPHVSG 548
           ARGPNGLNPEILKPD+IAPGVNILAAWTDAVGPTGLDFD RKTEFNILSGTSMACPHVSG
Sbjct: 496 ARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDKRKTEFNILSGTSMACPHVSG 555

Query: 549 AAALLKSAHPDWSPAALRSAMMTTASITDNRGQPMTEESTGKPSTPYDFGAGHVNLGLAM 608
           AAALLKSAHPDWSPAALRSAMMTTASITDNR QPMTEESTGKPSTPYDFGAGHVNLGLAM
Sbjct: 556 AAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKPSTPYDFGAGHVNLGLAM 615

Query: 609 DPGLVYDITNTDYVNFLCSLGYGPKMIQVITRTPVSCPAKKPLPENLNYPSIAAVFSTLS 668
           DPGL+YDITNTDY+NFLCS+GYGPKMIQVITRTPV CP KKPLPENLNYPSI  VFS+LS
Sbjct: 616 DPGLIYDITNTDYINFLCSIGYGPKMIQVITRTPVRCPTKKPLPENLNYPSIVTVFSSLS 675

Query: 669 KGWSTKAFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAAVKKQSYAVTVSADS 728
           KGWSTK+FIRT TNVGP+NSVYR KIEAPKGVTVKVKPSKLVFS  VKKQS+ V +SAD+
Sbjct: 676 KGWSTKSFIRTATNVGPSNSVYRVKIEAPKGVTVKVKPSKLVFSTTVKKQSFVVAISADN 735

Query: 729 QNLALGDAGAVFGWLSWSDGKHVVRSPLVVTQIEPL 765
           QNLALGD GAVFGWLSWSDGKHVVRSPLVVTQ+EPL
Sbjct: 736 QNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 771

BLAST of MS020632 vs. ExPASy TrEMBL
Match: A0A5D3BLE2 (Subtilisin-like protease SBT1.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001210 PE=3 SV=1)

HSP 1 Score: 1409.8 bits (3648), Expect = 0.0e+00
Identity = 693/741 (93.52%), Postives = 720/741 (97.17%), Query Frame = 0

Query: 24  TFIFRVDHFLKPSVFPTHFHWYTSEFAESPKILHVYDTVFHGFSATLTQQQVDSIGKHPS 83
           TFI R+D F KPSVFPTH+HWYTSEF +SP+ILHVYDTVFHGFSATLTQ+QVDSIGKHPS
Sbjct: 2   TFIVRIDRFSKPSVFPTHYHWYTSEFTQSPQILHVYDTVFHGFSATLTQEQVDSIGKHPS 61

Query: 84  VLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNL 143
           VLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNL
Sbjct: 62  VLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNL 121

Query: 144 GPVPRRWKGACETGTKFTARNCNRKIVGARFFSKGHEAGANAAGPIIGINDTIEYRSPRD 203
           GP+PRRWKG CETGTKFTA+NCNRKIVGARFFSKGHEAGANAAGPIIGINDTIEYRSPRD
Sbjct: 122 GPIPRRWKGVCETGTKFTAKNCNRKIVGARFFSKGHEAGANAAGPIIGINDTIEYRSPRD 181

Query: 204 ADGHGTHTASTAAGRYAFQASLEGYASGIAKGVAPKARLAIYKVCWKNSGCFDSDILAAF 263
           ADGHGTHTASTAAGR++FQASLEGYASGIAKGVAPKARLA+YKVCWKNSGCFDSDILAAF
Sbjct: 182 ADGHGTHTASTAAGRHSFQASLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAF 241

Query: 264 DAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAASRGVFVSSSAGNDGPNGMSVTN 323
           DAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAAS+GVFVSSSAGNDGPNGMSVTN
Sbjct: 242 DAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTN 301

Query: 324 LAPWITTVGAGTIDRNFPAVVTLGNGRKVSGVSLYAGAPLNGKMYPLVYPGKSGVLSVSL 383
           LAPW+TTVGAGTIDRNFP+VVTLGNGRK+ GVSLYAGAPLNG MYPLVYPGKSGVLSVSL
Sbjct: 302 LAPWVTTVGAGTIDRNFPSVVTLGNGRKIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSL 361

Query: 384 CMENSLDSKLVTGKIVICDRGSNPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLP 443
           CMENSLD K+VTGKIVICDRGS+PRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLP
Sbjct: 362 CMENSLDPKVVTGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLP 421

Query: 444 ACAVGSDEGDAMKAYASSSANPTATIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPD 503
           ACAVGSDEGDAMKAYASSS NPTATIAFQGTIIGIKPAPVVASFSARGPNGL PEILKPD
Sbjct: 422 ACAVGSDEGDAMKAYASSSTNPTATIAFQGTIIGIKPAPVVASFSARGPNGLTPEILKPD 481

Query: 504 LIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPA 563
           +IAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPA
Sbjct: 482 IIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPA 541

Query: 564 ALRSAMMTTASITDNRGQPMTEESTGKPSTPYDFGAGHVNLGLAMDPGLVYDITNTDYVN 623
           ALRSAMMTTASI DNR QPMTEESTGKPSTPYDFGAGHVNLGLAMDPGL+YDITNTDY+N
Sbjct: 542 ALRSAMMTTASIIDNRRQPMTEESTGKPSTPYDFGAGHVNLGLAMDPGLIYDITNTDYIN 601

Query: 624 FLCSLGYGPKMIQVITRTPVSCPAKKPLPENLNYPSIAAVFSTLSKGWSTKAFIRTVTNV 683
           FLCS+GYGPKMIQVITRTPV CP KKPLPENLNYPSI AVFS+LSKGWSTK+FIRTVTNV
Sbjct: 602 FLCSIGYGPKMIQVITRTPVRCPTKKPLPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNV 661

Query: 684 GPANSVYRAKIEAPKGVTVKVKPSKLVFSAAVKKQSYAVTVSADSQNLALGDAGAVFGWL 743
           GP+NSVYR KIEAPKGVTVKVKPSKLVFS  VKKQS+ V +SAD+QNLALGD GAVFGWL
Sbjct: 662 GPSNSVYRVKIEAPKGVTVKVKPSKLVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWL 721

Query: 744 SWSDGKHVVRSPLVVTQIEPL 765
           SWSDGKHVVRSPLVVTQ+EPL
Sbjct: 722 SWSDGKHVVRSPLVVTQLEPL 742

BLAST of MS020632 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 1156.7 bits (2991), Expect = 0.0e+00
Identity = 565/766 (73.76%), Postives = 662/766 (86.42%), Query Frame = 0

Query: 1   SAISLPLFFVFLLWTVSADSTLKTFIFRVDHFLKPSVFPTHFHWYTSEFAESPKILHVYD 60
           S I L LF  F   + +A    KTFIFR+D    PS+FPTH+HWY++EFAE  +I+HVY 
Sbjct: 4   STIVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEESRIVHVYH 63

Query: 61  TVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGS 120
           TVFHGFSA +T  + D++  HP+VLAVFEDRRR+LHTTRSPQFLGL+NQ+GLWS+SDYGS
Sbjct: 64  TVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSESDYGS 123

Query: 121 DVIIGVFDTGISPERRSFSDVNLGPVPRRWKGACETGTKFTARNCNRKIVGARFFSKGHE 180
           DVIIGVFDTGI PERRSFSD+NLGP+P+RW+G CE+G +F+ RNCNRKI+GARFF+KG +
Sbjct: 124 DVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQ 183

Query: 181 AGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRYAFQASLEGYASGIAKGVAPKA 240
           A       I GIN T+E+ SPRDADGHGTHT+STAAGR+AF+AS+ GYASG+AKGVAPKA
Sbjct: 184 AAV-----IGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKA 243

Query: 241 RLAIYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGA 300
           R+A YKVCWK+SGC DSDILAAFDAAV DGVDVISISIGGGDG++SPYYLDPIAIG+YGA
Sbjct: 244 RIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGA 303

Query: 301 ASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGRKVSGVSLYAG 360
           AS+G+FVSSSAGN+GPNGMSVTNLAPW+TTVGA TIDRNFPA   LG+G ++ GVSLYAG
Sbjct: 304 ASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAG 363

Query: 361 APLNGKMYPLVYPGKSGVLSVSLCMENSLDSKLVTGKIVICDRGSNPRVAKGLVVKKAGG 420
            PLNG+M+P+VYPGKSG+ S SLCMEN+LD K V GKIVICDRGS+PRVAKGLVVKKAGG
Sbjct: 364 VPLNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGG 423

Query: 421 VGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSANPTATIAFQGTIIGIKP 480
           VGMILANG SNGEGLVGDAHL+PACAVGS+EGD +KAYASS  NP A+I F+GTI+GIKP
Sbjct: 424 VGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKP 483

Query: 481 APVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTS 540
           APV+ASFS RGPNGL+PEILKPDLIAPGVNILAAWTDAVGPTGL  D RKTEFNILSGTS
Sbjct: 484 APVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTS 543

Query: 541 MACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRGQPMTEESTGKPSTPYDFGAG 600
           MACPHVSGAAALLKSAHPDWSPA +RSAMMTT ++ DN  + + +ESTGK +TPYD+G+G
Sbjct: 544 MACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGSG 603

Query: 601 HVNLGLAMDPGLVYDITNTDYVNFLCSLGYGPKMIQVITRTPVSCP-AKKPLPENLNYPS 660
           H+NLG AM+PGLVYDITN DY+ FLCS+GYGPK IQVITRTPV CP  +KP P NLNYPS
Sbjct: 604 HLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPS 663

Query: 661 IAAVFSTLSKGWSTKAFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAAVKKQS 720
           I AVF T  +G  +K  IRT TNVG A +VYRA+IE+P+GVTV VKP +LVF++AVK++S
Sbjct: 664 ITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRS 723

Query: 721 YAVTVSADSQNLALGDAGAVFGWLSWSD-GKHVVRSPLVVTQIEPL 765
           YAVTV+ +++N+ LG+ GAVFG ++W D GKHVVRSP+VVTQ++ L
Sbjct: 724 YAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVTQMDTL 764

BLAST of MS020632 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 858.2 bits (2216), Expect = 5.0e-249
Identity = 455/781 (58.26%), Postives = 550/781 (70.42%), Query Frame = 0

Query: 8   FFVFLLWTVSADSTLK------TFIFRVDHFLKPSVFPTHFHWYTSEFA----ESPKILH 67
           F+ F L T+S+ S+        T+I  VDH  KPS+FPTHFHWYTS  A      P I+H
Sbjct: 5   FYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSIIH 64

Query: 68  VYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLR--NQRGLWSD 127
            YDTVFHGFSA LT Q    +  HP V++V  ++ R LHTTRSP+FLGLR  ++ GL  +
Sbjct: 65  TYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEE 124

Query: 128 SDYGSDVIIGVFDTGISPERRSFSDVNLGPVPRRWKGACETGTKFTARNCNRKIVGARFF 187
           SD+GSD++IGV DTG+ PER SF D  LGPVP +WKG C     F    CNRK+VGARFF
Sbjct: 125 SDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFF 184

Query: 188 SKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRYAFQASLEGYASGIAKG 247
             G+EA          +N+T E+RSPRD+DGHGTHTAS +AGRY F AS  GYA G+A G
Sbjct: 185 CGGYEATNGK------MNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAG 244

Query: 248 VAPKARLAIYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAI 307
           +APKARLA YKVCW NSGC+DSDILAAFD AV DGVDVIS+S+G   GV  PYYLD IAI
Sbjct: 245 MAPKARLAAYKVCW-NSGCYDSDILAAFDTAVADGVDVISLSVG---GVVVPYYLDAIAI 304

Query: 308 GAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGRKVSGV 367
           GA+GA  RG+FVS+SAGN GP  ++VTN+APW+TTVGAGTIDR+FPA V LGNG+ +SGV
Sbjct: 305 GAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGV 364

Query: 368 SLYAGAPLN-GKMYPLVYPGK---SGVLSVSLCMENSLDSKLVTGKIVICDRGSNPRVAK 427
           S+Y G  L+ G+MYPLVY G        S SLC+E SLD  LV GKIV+CDRG N R  K
Sbjct: 365 SVYGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATK 424

Query: 428 GLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYAS------SSANP 487
           G +V+K GG+GMI+ANG+ +GEGLV D H+LPA +VG+  GD ++ Y S      SS +P
Sbjct: 425 GEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHP 484

Query: 488 TATIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLD 547
           TATI F+GT +GI+PAPVVASFSARGPN   PEILKPD+IAPG+NILAAW D +GP+G+ 
Sbjct: 485 TATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVT 544

Query: 548 FDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRGQPMTE 607
            D R+TEFNILSGTSMACPHVSG AALLK+AHPDWSPAA+RSA++TTA   DN G+PM +
Sbjct: 545 SDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMD 604

Query: 608 ESTGKPSTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSLGYGPKMIQVITRTPVSC 667
           ESTG  S+  D+G+GHV+   AMDPGLVYDIT+ DY+NFLC+  Y    I  ITR    C
Sbjct: 605 ESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADC 664

Query: 668 PAKKPLPE--NLNYPSIAAVFSTLSKGWSTKAFIRTVTNVGPANSVYRAKIEAPKGVTVK 727
              +      NLNYPS + VF    +   +  FIRTVTNVG ++SVY  KI  P+G TV 
Sbjct: 665 DGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVT 724

Query: 728 VKPSKLVFSAAVKKQSYAVTVSADSQNLALGDAGAVFGWLSWSDGKHVVRSPLVVTQIEP 765
           V+P KL F    +K S+ V V      L+ G      G + WSDGK  V SPLVVT  +P
Sbjct: 725 VEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQQP 775

BLAST of MS020632 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 741.9 bits (1914), Expect = 5.2e-214
Identity = 399/770 (51.82%), Postives = 526/770 (68.31%), Query Frame = 0

Query: 1   SAISLPLFFVFL----LWTVSADSTLKTFIFRVDHFLKPSVFPTHFHWYTS---EFAESP 60
           S +S   FF+ L        S+ S   T+I  +     PS F  H +WY S     ++S 
Sbjct: 4   SFLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSA 63

Query: 61  KILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGL-RNQRGL 120
           ++L+ Y+   HGFS  LTQ++ DS+   P V++V  + R +LHTTR+P FLGL  +   L
Sbjct: 64  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 123

Query: 121 WSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPVPRRWKGACETGTKFTARNCNRKIVGA 180
           + ++   SDV++GV DTG+ PE +S+SD   GP+P  WKG CE GT FTA  CNRK++GA
Sbjct: 124 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 183

Query: 181 RFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRYAFQASLEGYASGI 240
           RFF++G+E   +  GP   I+++ E RSPRD DGHGTHT+STAAG     ASL GYASG 
Sbjct: 184 RFFARGYE---STMGP---IDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGT 243

Query: 241 AKGVAPKARLAIYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDP 300
           A+G+AP+AR+A+YKVCW   GCF SDILAA D A+ D V+V+S+S+GGG    S YY D 
Sbjct: 244 ARGMAPRARVAVYKVCWL-GGCFSSDILAAIDKAIADNVNVLSMSLGGG---MSDYYRDG 303

Query: 301 IAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGRKV 360
           +AIGA+ A  RG+ VS SAGN GP+  S++N+APWITTVGAGT+DR+FPA+  LGNG+  
Sbjct: 304 VAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNF 363

Query: 361 SGVSLYAGAPLNGKMYPLVYPGK-SGVLSVSLCMENSLDSKLVTGKIVICDRGSNPRVAK 420
           +GVSL+ G  L  K+ P +Y G  S   + +LCM  +L  + V GKIV+CDRG N RV K
Sbjct: 364 TGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQK 423

Query: 421 GLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSANPTATIAF 480
           G VVK AGGVGMILAN  +NGE LV DAHLLPA  VG   GD ++ Y ++  NPTA+I+ 
Sbjct: 424 GDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISI 483

Query: 481 QGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKT 540
            GT++G+KP+PVVA+FS+RGPN + P ILKPDLIAPGVNILAAWT A GPTGL  D+R+ 
Sbjct: 484 LGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRV 543

Query: 541 EFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRGQPMTEESTGKP 600
           EFNI+SGTSM+CPHVSG AALLKS HP+WSPAA+RSA+MTTA  T   G+P+ + +TGKP
Sbjct: 544 EFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKP 603

Query: 601 STPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSLGYGPKMIQVITRTPVSC-PAKKP 660
           STP+D GAGHV+   A +PGL+YD+T  DY+ FLC+L Y    I+ ++R   +C P+K  
Sbjct: 604 STPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSY 663

Query: 661 LPENLNYPSIAAVFSTLSKGWSTKAFIRTVTNVGPANSVYRAKIEA-PKGVTVKVKPSKL 720
              +LNYPS    F+    G     + RTVT+VG A + Y  K+ +   GV + V+P+ L
Sbjct: 664 SVADLNYPS----FAVNVDGVGAYKYTRTVTSVGGAGT-YSVKVTSETTGVKISVEPAVL 723

Query: 721 VFSAAVKKQSYAVTVSADSQNLALGDAGAVFGWLSWSDGKHVVRSPLVVT 760
            F  A +K+SY VT + DS   +  ++   FG + WSDGKHVV SP+ ++
Sbjct: 724 NFKEANEKKSYTVTFTVDSSKPSGSNS---FGSIEWSDGKHVVGSPVAIS 755

BLAST of MS020632 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 736.1 bits (1899), Expect = 2.9e-212
Identity = 386/758 (50.92%), Postives = 516/758 (68.07%), Query Frame = 0

Query: 1   SAISLPLFFVFLLWTVSADSTLKTFIFRVDHFLKPSVFPTHFHWYTSEFAESPKILHVYD 60
           S+I++   F+FLL   +A    KT+I RV+H  KP  F TH  WYTS+      +L+ Y 
Sbjct: 9   SSITIITTFLFLLLHTTAK---KTYIIRVNHSDKPESFLTHHDWYTSQLNSESSLLYTYT 68

Query: 61  TVFHGFSATLTQQQVDS-IGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYG 120
           T FHGFSA L   + DS +    S+L +FED    LHTTR+P+FLGL ++ G+       
Sbjct: 69  TSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSS 128

Query: 121 SDVIIGVFDTGISPERRSFSDVNLGPVPRRWKGACETGTKFTARNCNRKIVGARFFSKGH 180
           + VIIGV DTG+ PE RSF D ++  +P +WKG CE+G+ F ++ CN+K++GAR FSKG 
Sbjct: 129 NGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGF 188

Query: 181 EAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRYAFQASLEGYASGIAKGVAPK 240
           +  +       G +   E  SPRD DGHGTHT++TAAG     AS  GYA+G A+G+A +
Sbjct: 189 QMASGG-----GFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATR 248

Query: 241 ARLAIYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYG 300
           AR+A YKVCW ++GCF SDILAA D A+ DGVDV+S+S+GGG   S+PYY D IAIGA+ 
Sbjct: 249 ARVATYKVCW-STGCFGSDILAAMDRAILDGVDVLSLSLGGG---SAPYYRDTIAIGAFS 308

Query: 301 AASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGRKVSGVSLYA 360
           A  RGVFVS SAGN GP   SV N+APW+ TVGAGT+DR+FPA   LGNG++++GVSLY+
Sbjct: 309 AMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYS 368

Query: 361 GAPLNGKMYPLVYPGKSGVLSVSLCMENSLDSKLVTGKIVICDRGSNPRVAKGLVVKKAG 420
           G  +  K   LVY  K    S +LC+  SLDS +V GKIV+CDRG N RV KG VV+ AG
Sbjct: 369 GVGMGTKPLELVY-NKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAG 428

Query: 421 GVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSANPTATIAFQGTIIGIK 480
           G+GMI+AN  ++GE LV D+HLLPA AVG   GD ++ Y  S + PTA + F+GT++ +K
Sbjct: 429 GLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVK 488

Query: 481 PAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGT 540
           P+PVVA+FS+RGPN + PEILKPD+I PGVNILA W+DA+GPTGLD D+R+T+FNI+SGT
Sbjct: 489 PSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGT 548

Query: 541 SMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRGQPMTEESTGKPSTPYDFGA 600
           SM+CPH+SG A LLK+AHP+WSP+A++SA+MTTA + DN   P+ + +    S PY  G+
Sbjct: 549 SMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGS 608

Query: 601 GHVNLGLAMDPGLVYDITNTDYVNFLCSLGYG-PKMIQVITRTPVSCPAKKPLPENLNYP 660
           GHV+   A+ PGLVYDI+  +Y+ FLCSL Y    ++ ++ R  V+C  K   P  LNYP
Sbjct: 609 GHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYP 668

Query: 661 SIAAVFSTLSKGWSTKAFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAAVKKQ 720
           S    FS L  G     + R VTNVG A+SVY+  +     V + VKPSKL F +  +K+
Sbjct: 669 S----FSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKK 728

Query: 721 SYAVTVSADSQNLALGDAGAVFGWLSWSDGKHVVRSPL 757
            Y VT     + +++ +  A FG ++WS+ +H VRSP+
Sbjct: 729 RYTVTF-VSKKGVSMTNK-AEFGSITWSNPQHEVRSPV 747

BLAST of MS020632 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 703.4 bits (1814), Expect = 2.0e-202
Identity = 393/787 (49.94%), Postives = 507/787 (64.42%), Query Frame = 0

Query: 3   ISLPLFFVFL-LWTVSADSTLKTFIFRVDHFLKPSVFPTHFHWYTS------------EF 62
           I L +  +FL   T +  ST KT++  +D    P  +  H  WY+S            E 
Sbjct: 14  IILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEE 73

Query: 63  AESPKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQ 122
             + +IL+ Y T FHG +A LTQ++ + + +   V+AV  + R +LHTTRSP FLGL  Q
Sbjct: 74  GNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQ 133

Query: 123 RG--LWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPVPRRWKGACETGTKFTARNCNR 182
               +W++     DV++GV DTGI PE  SF+D  + PVP  W+GACETG +F  RNCNR
Sbjct: 134 ESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNR 193

Query: 183 KIVGARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRYAFQASLEG 242
           KIVGAR F +G+EA          I++ +EY+SPRD DGHGTHTA+T AG     A+L G
Sbjct: 194 KIVGARVFYRGYEAATGK------IDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFG 253

Query: 243 YASGIAKGVAPKARLAIYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSP 302
           +A G A+G+A KAR+A YKVCW   GCF SDIL+A D AV DGV V+SIS+GGG    S 
Sbjct: 254 FAYGTARGMAQKARVAAYKVCWV-GGCFSSDILSAVDQAVADGVQVLSISLGGG---VST 313

Query: 303 YYLDPIAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLG 362
           Y  D ++I  +GA   GVFVS SAGN GP+ +S+TN++PWITTVGA T+DR+FPA V +G
Sbjct: 314 YSRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIG 373

Query: 363 NGRKVSGVSLYAGAPL--NGKMYPLVYPGK--SGVLSVSLCMENSLDSKLVTGKIVICDR 422
             R   GVSLY G  +    K YPLVY G+  S     S C++ +LD + V GKIVICDR
Sbjct: 374 TMRTFKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDR 433

Query: 423 GSNPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSA 482
           G  PRV KG VVK+AGG+GM+L N  +NGE LV D+H+LPA AVG  EG  +K YA +S 
Sbjct: 434 GVTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSK 493

Query: 483 NPTATIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTG 542
             TA++   GT IGIKP+PVVA+FS+RGPN L+ EILKPDL+APGVNILAAWT  + P+ 
Sbjct: 494 KATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSS 553

Query: 543 LDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRGQPM 602
           L  D R+ +FNILSGTSM+CPHVSG AAL+KS HPDWSPAA++SA+MTTA + DN  +P+
Sbjct: 554 LSSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPL 613

Query: 603 TEESTGKPSTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSLGYGPKMIQVIT---- 662
           T+ S   PS+PYD GAGH++   A DPGLVYDI   +Y  FLC+    P  ++V T    
Sbjct: 614 TDASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSN 673

Query: 663 RTPVSCPAKKPLPENLNYPSIAAVF--STLSKGWSTKAFIRTVTNVGPANSVYRAKIEAP 722
           RT     AK   P NLNYP+I+A+F  +T  K  + +   RTVTNVGP  S Y+  +   
Sbjct: 674 RTCKHTLAKN--PGNLNYPAISALFPENTHVKAMTLR---RTVTNVGPHISSYKVSVSPF 733

Query: 723 KGVTVKVKPSKLVFSAAVKKQSYAVTVSADSQNLALGDAGAVFGWLSWSDGKHVVRSPLV 765
           KG +V V+P  L F++  +K SY VT     +          FG L W    H VRSP++
Sbjct: 734 KGASVTVQPKTLNFTSKHQKLSYTVTFRTRFRM-----KRPEFGGLVWKSTTHKVRSPVI 780