MS020615 (gene) Bitter gourd (TR) v1

Overview
NameMS020615
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionGlutamyl-tRNA(Gln) amidotransferase subunit A
Locationscaffold375: 1657994 .. 1660063 (+)
RNA-Seq ExpressionMS020615
SyntenyMS020615
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAACTCCACGCTTCTCTCTTTCATTTTCTTTGTTTCTGATTTTGGTAGCAGTATCACCGTTCGGGTCTTACAGTGTAAGGCCGGCTGGCTTCCCCATTGAAGAAGCCGGATTGAAAGATTTGCACCTAGCTTTCAAACAAAACAAACTCACCTCCAGGCAACTTGTTGAGTTTTACATTAAGCAAATCCGTAGATATAACCCACGGCTTAGAGGGGTGATTGAGGTGAATCCAGATGCATTATACTTAGCCGACAAAGCAGACCGTGAGCGCAAGGCCAAGGCGCCAGGCTCACTGCCTCTTTTGCACGGCATTCCTGTGCTTGTAAAAGATAACATGGCCACAAAGGACAAGCTCAACACCACGGCTGGCTCATTGGCTCTGTTGGGCTCCGTTGTGCCTCGTGATGCCGGAGCTGTGATGAGGTTGAGGAAAGCTGGAGCAATCATTTTGGGAAAGGCCAGCATGAGCGAATGGGCTGGTTTTAGATCCAATGGAGCTCCCACTGGCTGGAACGCCCGGGCAGGACAAGGCCGGGTGAGTGAGCCTCCAACTCCTCCAATACTTCTTCCATTATATTTGTGTAAAAGAAAAGGGCATCAATATTCAATTCTTTGTTCAAACTTTCTTGGAAGTTCCTTGCTACAATCCAATTAAAGTTATCTCTTGTAAGTGACAAATTGATTTGAATCTCAAATATACAAAACTAAGACTCAAGGTAAAAATGGTGCAGGAACCTTATACATTAGGACTTCCTTGTGGTTCAAGCAGCGGCTCGGCAATATCAGTGTCGGCAAACATGGCCGCTGTCACACTAGGAACTGAAACGGATGGCTCAATACTATGTCCTTCTAGTTTTAACTCAGTAGTTGGCATCAAACCAACCCTTGGCCTCACTAGTACAGCCGGAGTCGTCCCGATCTCTCCACGACAAGATACAGTTGGGTAAGTGTTCCAAAAAATATTATTCAAAACTAGATGATCTCATTCAGTATTCAAGTCCTATTCACTTATTTAATTGATTTGATTTTACAATAACGTGATCATGTAAATAGAGAATAATTCCACTCTTGCTACATGAATGAACATGGGAACAGGCCAATCTGTAGAACGGTTTCCGATGCTGCTTATGTTCTCGACGTCATTGTAGGAACTGACCCCCTGGATAACTCAACCTATAAAGCATCTCGGTATATTCCAAGGGGTGGGTACGGTCAATTTCTTAAGGCTGATGGATTAAGAGGAAAAAGATTGGGAATAGTGGAGGATTTCTTTGGTGTAGTTGATCCTTCCTTAATTCCAGCTTTTGAGGAAATTTTCACAATATTAAGGTACCAAAAGATAGTTTCCAAATTGCAAAGATTATTTGCAATTATACTCGAACTTTTCTAATGTTTCATGGCAGTTTTGTGTTAGTATTAACGAAAAGGGATTTATGCAGCAAACGAGGTGCAATATTGGTAGACAATTTGAAAATAGACAACCTTGGAGCCATATATAACAATACAGAAAGTGGAGAAGTAGCTGCATTGCTGAATGAGTTCAAAGTGTCCTTAAATGCATACCTGAAAGAGCTGGTTTCCTCTCCAATTCGATCTTTAGCGGAAGCCATAGCATTCAACCAAAAGCACTCGAGCCTGGTAATTAAGCATGCTTGATTCTGTTGTTACATTCGAATCAAAACTCAATAGCAATTGAACTTCGTGTTTCATTTACAAAAAACAGGAGAAAATTAAAGAGTACGGTCAAGATTTATTTCTACAAGCTGAGGCGACAAATGGAATGGGAATGAACGGATTATTATCGAAATTAGGGAAACTTTCAAAAGATGGACTGGAGAAAACGATGATTAAGAATAAGCTAGATGCAATAATGACTCCGAGTGCGATAATCTCACCGATTCTAGCAATTGGAGGATTTCCCGGCATAACTGTTCCGGCAGGATATTCGCCCTCAGGGACGCCATTTGGCATTGATTTCGGAGGTTTGAAAGGATACGAGCCGAAACTGATAGAAATTGCATATGGATTTGAGCAAGCAACCAAGAGAAGAAGAAAGCCTCCCCTC

mRNA sequence

ATGGAAACTCCACGCTTCTCTCTTTCATTTTCTTTGTTTCTGATTTTGGTAGCAGTATCACCGTTCGGGTCTTACAGTGTAAGGCCGGCTGGCTTCCCCATTGAAGAAGCCGGATTGAAAGATTTGCACCTAGCTTTCAAACAAAACAAACTCACCTCCAGGCAACTTGTTGAGTTTTACATTAAGCAAATCCGTAGATATAACCCACGGCTTAGAGGGGTGATTGAGGTGAATCCAGATGCATTATACTTAGCCGACAAAGCAGACCGTGAGCGCAAGGCCAAGGCGCCAGGCTCACTGCCTCTTTTGCACGGCATTCCTGTGCTTGTAAAAGATAACATGGCCACAAAGGACAAGCTCAACACCACGGCTGGCTCATTGGCTCTGTTGGGCTCCGTTGTGCCTCGTGATGCCGGAGCTGTGATGAGGTTGAGGAAAGCTGGAGCAATCATTTTGGGAAAGGCCAGCATGAGCGAATGGGCTGGTTTTAGATCCAATGGAGCTCCCACTGGCTGGAACGCCCGGGCAGGACAAGGCCGGGAACCTTATACATTAGGACTTCCTTGTGGTTCAAGCAGCGGCTCGGCAATATCAGTGTCGGCAAACATGGCCGCTGTCACACTAGGAACTGAAACGGATGGCTCAATACTATGTCCTTCTAGTTTTAACTCAGTAGTTGGCATCAAACCAACCCTTGGCCTCACTAGTACAGCCGGAGTCGTCCCGATCTCTCCACGACAAGATACAGTTGGGCCAATCTGTAGAACGGTTTCCGATGCTGCTTATGTTCTCGACGTCATTGTAGGAACTGACCCCCTGGATAACTCAACCTATAAAGCATCTCGGTATATTCCAAGGGGTGGGTACGGTCAATTTCTTAAGGCTGATGGATTAAGAGGAAAAAGATTGGGAATAGTGGAGGATTTCTTTGGTGTAGTTGATCCTTCCTTAATTCCAGCTTTTGAGGAAATTTTCACAATATTAAGCAAACGAGGTGCAATATTGGTAGACAATTTGAAAATAGACAACCTTGGAGCCATATATAACAATACAGAAAGTGGAGAAGTAGCTGCATTGCTGAATGAGTTCAAAGTGTCCTTAAATGCATACCTGAAAGAGCTGGTTTCCTCTCCAATTCGATCTTTAGCGGAAGCCATAGCATTCAACCAAAAGCACTCGAGCCTGGAGAAAATTAAAGAGTACGGTCAAGATTTATTTCTACAAGCTGAGGCGACAAATGGAATGGGAATGAACGGATTATTATCGAAATTAGGGAAACTTTCAAAAGATGGACTGGAGAAAACGATGATTAAGAATAAGCTAGATGCAATAATGACTCCGAGTGCGATAATCTCACCGATTCTAGCAATTGGAGGATTTCCCGGCATAACTGTTCCGGCAGGATATTCGCCCTCAGGGACGCCATTTGGCATTGATTTCGGAGGTTTGAAAGGATACGAGCCGAAACTGATAGAAATTGCATATGGATTTGAGCAAGCAACCAAGAGAAGAAGAAAGCCTCCCCTC

Coding sequence (CDS)

ATGGAAACTCCACGCTTCTCTCTTTCATTTTCTTTGTTTCTGATTTTGGTAGCAGTATCACCGTTCGGGTCTTACAGTGTAAGGCCGGCTGGCTTCCCCATTGAAGAAGCCGGATTGAAAGATTTGCACCTAGCTTTCAAACAAAACAAACTCACCTCCAGGCAACTTGTTGAGTTTTACATTAAGCAAATCCGTAGATATAACCCACGGCTTAGAGGGGTGATTGAGGTGAATCCAGATGCATTATACTTAGCCGACAAAGCAGACCGTGAGCGCAAGGCCAAGGCGCCAGGCTCACTGCCTCTTTTGCACGGCATTCCTGTGCTTGTAAAAGATAACATGGCCACAAAGGACAAGCTCAACACCACGGCTGGCTCATTGGCTCTGTTGGGCTCCGTTGTGCCTCGTGATGCCGGAGCTGTGATGAGGTTGAGGAAAGCTGGAGCAATCATTTTGGGAAAGGCCAGCATGAGCGAATGGGCTGGTTTTAGATCCAATGGAGCTCCCACTGGCTGGAACGCCCGGGCAGGACAAGGCCGGGAACCTTATACATTAGGACTTCCTTGTGGTTCAAGCAGCGGCTCGGCAATATCAGTGTCGGCAAACATGGCCGCTGTCACACTAGGAACTGAAACGGATGGCTCAATACTATGTCCTTCTAGTTTTAACTCAGTAGTTGGCATCAAACCAACCCTTGGCCTCACTAGTACAGCCGGAGTCGTCCCGATCTCTCCACGACAAGATACAGTTGGGCCAATCTGTAGAACGGTTTCCGATGCTGCTTATGTTCTCGACGTCATTGTAGGAACTGACCCCCTGGATAACTCAACCTATAAAGCATCTCGGTATATTCCAAGGGGTGGGTACGGTCAATTTCTTAAGGCTGATGGATTAAGAGGAAAAAGATTGGGAATAGTGGAGGATTTCTTTGGTGTAGTTGATCCTTCCTTAATTCCAGCTTTTGAGGAAATTTTCACAATATTAAGCAAACGAGGTGCAATATTGGTAGACAATTTGAAAATAGACAACCTTGGAGCCATATATAACAATACAGAAAGTGGAGAAGTAGCTGCATTGCTGAATGAGTTCAAAGTGTCCTTAAATGCATACCTGAAAGAGCTGGTTTCCTCTCCAATTCGATCTTTAGCGGAAGCCATAGCATTCAACCAAAAGCACTCGAGCCTGGAGAAAATTAAAGAGTACGGTCAAGATTTATTTCTACAAGCTGAGGCGACAAATGGAATGGGAATGAACGGATTATTATCGAAATTAGGGAAACTTTCAAAAGATGGACTGGAGAAAACGATGATTAAGAATAAGCTAGATGCAATAATGACTCCGAGTGCGATAATCTCACCGATTCTAGCAATTGGAGGATTTCCCGGCATAACTGTTCCGGCAGGATATTCGCCCTCAGGGACGCCATTTGGCATTGATTTCGGAGGTTTGAAAGGATACGAGCCGAAACTGATAGAAATTGCATATGGATTTGAGCAAGCAACCAAGAGAAGAAGAAAGCCTCCCCTC

Protein sequence

METPRFSLSFSLFLILVAVSPFGSYSVRPAGFPIEEAGLKDLHLAFKQNKLTSRQLVEFYIKQIRRYNPRLRGVIEVNPDALYLADKADRERKAKAPGSLPLLHGIPVLVKDNMATKDKLNTTAGSLALLGSVVPRDAGAVMRLRKAGAIILGKASMSEWAGFRSNGAPTGWNARAGQGREPYTLGLPCGSSSGSAISVSANMAAVTLGTETDGSILCPSSFNSVVGIKPTLGLTSTAGVVPISPRQDTVGPICRTVSDAAYVLDVIVGTDPLDNSTYKASRYIPRGGYGQFLKADGLRGKRLGIVEDFFGVVDPSLIPAFEEIFTILSKRGAILVDNLKIDNLGAIYNNTESGEVAALLNEFKVSLNAYLKELVSSPIRSLAEAIAFNQKHSSLEKIKEYGQDLFLQAEATNGMGMNGLLSKLGKLSKDGLEKTMIKNKLDAIMTPSAIISPILAIGGFPGITVPAGYSPSGTPFGIDFGGLKGYEPKLIEIAYGFEQATKRRRKPPL
Homology
BLAST of MS020615 vs. NCBI nr
Match: XP_022146229.1 (putative amidase C869.01 [Momordica charantia])

HSP 1 Score: 978.0 bits (2527), Expect = 3.1e-281
Identity = 505/509 (99.21%), Postives = 506/509 (99.41%), Query Frame = 0

Query: 1   METPRFSLSFSLFLILVAVSPFGSYSVRPAGFPIEEAGLKDLHLAFKQNKLTSRQLVEFY 60
           METPR SLSFSLFLILVAVSPF SYSVRPAGFPIEEAGLKDLHLAFKQNKLTSRQLVEFY
Sbjct: 1   METPRLSLSFSLFLILVAVSPFWSYSVRPAGFPIEEAGLKDLHLAFKQNKLTSRQLVEFY 60

Query: 61  IKQIRRYNPRLRGVIEVNPDALYLADKADRERKAKAPGSLPLLHGIPVLVKDNMATKDKL 120
           IKQIRRYNPRLRGVIEVNPDALYLADKADRERKAKAPGSLPLLHGIPVLVKDNMATKDKL
Sbjct: 61  IKQIRRYNPRLRGVIEVNPDALYLADKADRERKAKAPGSLPLLHGIPVLVKDNMATKDKL 120

Query: 121 NTTAGSLALLGSVVPRDAGAVMRLRKAGAIILGKASMSEWAGFRSNGAPTGWNARAGQGR 180
           NTTAGSLALLGSVVPRDAGAVMRLRKAGAIILGKASMSEWAGFRSNGAPTGWNARAGQGR
Sbjct: 121 NTTAGSLALLGSVVPRDAGAVMRLRKAGAIILGKASMSEWAGFRSNGAPTGWNARAGQGR 180

Query: 181 EPYTLGLPCGSSSGSAISVSANMAAVTLGTETDGSILCPSSFNSVVGIKPTLGLTSTAGV 240
           EPYTLGLPCGSSSGSAISVSANMAAV LGTETDGSILCPSSFNSVVGIKPTLGLTSTAGV
Sbjct: 181 EPYTLGLPCGSSSGSAISVSANMAAVALGTETDGSILCPSSFNSVVGIKPTLGLTSTAGV 240

Query: 241 VPISPRQDTVGPICRTVSDAAYVLDVIVGTDPLDNSTYKASRYIPRGGYGQFLKADGLRG 300
           VPISPRQDTVGPICRTVSDAAYVLDVIVGTDPLDNSTYKASRYIPRGGYGQFLKADGLRG
Sbjct: 241 VPISPRQDTVGPICRTVSDAAYVLDVIVGTDPLDNSTYKASRYIPRGGYGQFLKADGLRG 300

Query: 301 KRLGIVEDFFGVVDPSLIPAFEEIFTILSKRGAILVDNLKIDNLGAIYNNTESGEVAALL 360
           KRLGIVEDFFGVVDPSLIPAFEEIFTILSKRGAILVDNLKIDNLGAIYNNTESGEVAALL
Sbjct: 301 KRLGIVEDFFGVVDPSLIPAFEEIFTILSKRGAILVDNLKIDNLGAIYNNTESGEVAALL 360

Query: 361 NEFKVSLNAYLKELVSSPIRSLAEAIAFNQKHSSLEKIKEYGQDLFLQAEATNGMGMNGL 420
           NEFKVSLNAYLKELVSSPIRSLAEAIAFNQKHSSLEKIKEYGQDLFLQAEATNGMG+NGL
Sbjct: 361 NEFKVSLNAYLKELVSSPIRSLAEAIAFNQKHSSLEKIKEYGQDLFLQAEATNGMGINGL 420

Query: 421 LSKLGKLSKDGLEKTMIKNKLDAIMTPSAIISPILAIGGFPGITVPAGYSPSGTPFGIDF 480
           LSKLGKLSKDGLEKTMIKNKLDAIMTPSAIISPILAIGGFPGITVPAGYSPSGTPFGIDF
Sbjct: 421 LSKLGKLSKDGLEKTMIKNKLDAIMTPSAIISPILAIGGFPGITVPAGYSPSGTPFGIDF 480

Query: 481 GGLKGYEPKLIEIAYGFEQATKRRRKPPL 510
           GGLKGYEPKLIEIAYGFEQATKRRRKPPL
Sbjct: 481 GGLKGYEPKLIEIAYGFEQATKRRRKPPL 509

BLAST of MS020615 vs. NCBI nr
Match: XP_038900913.1 (probable amidase At4g34880 [Benincasa hispida])

HSP 1 Score: 651.7 bits (1680), Expect = 5.1e-183
Identity = 349/514 (67.90%), Postives = 409/514 (79.57%), Query Frame = 0

Query: 1   METPRFSLSFSLFLILVAV-SPFGSYSVRPAGFPIEEAGLKDLHLAFKQNKLTSRQLVEF 60
           M    F L  S+ L LVA+ S +G+ S     FPIEEA +KDL LAF QNKLTSRQLV+F
Sbjct: 1   MAAQSFWLYISMVLSLVAILSSYGNCSF-GTHFPIEEATVKDLQLAFYQNKLTSRQLVKF 60

Query: 61  YIKQIRRYNPRLRGVIEVNPDALYLADKADRERKAKAPGSLPLLHGIPVLVKDNMATKDK 120
           Y+KQ+RR+NP L+G+IEVNPDAL  A +AD ERK  +  SL  LHGIPVL+KDN+ATKDK
Sbjct: 61  YLKQVRRFNPILKGIIEVNPDALDQATRADHERKRSSLSSLSPLHGIPVLLKDNIATKDK 120

Query: 121 LNTTAGSLALLGSVVPRDAGAVMRLRKAGAIILGKASMSEWAGFRSNGAPTGWNARAGQG 180
           LNTTAGS ALLGS+VPRDAG V +LRKAGAII GKAS+SEW+ FRSN  P GW AR GQG
Sbjct: 121 LNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSQFRSN-LPNGWCARGGQG 180

Query: 181 REPYTLGLPCGSSSGSAISVSANMAAVTLGTETDGSILCPSSFNSVVGIKPTLGLTSTAG 240
           + PYT+G PCGSSSGSAISV+ANM AV+LGT+TDGSILCPS++NSVVGI+PT+GLTS AG
Sbjct: 181 KNPYTMGEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTYNSVVGIRPTVGLTSRAG 240

Query: 241 VVPISPRQDTVGPICRTVSDAAYVLDVIVGTDPLDNSTYKASRYIPRGGYGQFLKADGLR 300
           VVPIS RQD VGPICRTVSDAAYVLD IVG D  DNST +AS+YIP+GGYGQFL+ DGL+
Sbjct: 241 VVPISSRQDAVGPICRTVSDAAYVLDAIVGADKYDNSTIEASKYIPKGGYGQFLRPDGLK 300

Query: 301 GKRLGIVEDFFGVVDPSLI--PAFEEIFTILSKRGAILVDNLKIDNLGAIYNNTESGEVA 360
           G R+GIV  FF   D  +    A+E++F  L + GAILVDNL ID+L  I     SGE  
Sbjct: 301 GTRIGIVRKFFDFGDDGVFYSEAYEKVFKSLKQGGAILVDNLTIDSLDIIIGGL-SGEWT 360

Query: 361 ALLNEFKVSLNAYLKELVSSPIRSLAEAIAFNQKHSSLEKIKEYGQDLFLQAEATNGMG- 420
           AL  EFK+S+NAYLKELV+SPIRSL++AI FN+K+S LEK+KEYGQ LFL+AEATNG+G 
Sbjct: 361 ALPAEFKISINAYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQVLFLEAEATNGIGD 420

Query: 421 -MNGLLSKLGKLSKDGLEKTMIKNKLDAIMTPSAIISPILAIGGFPGITVPAGYSPSGTP 480
                L+ +  LSK+G E+ MIKNKLDAI  P  +ISP+LAIGGFPGI+VPAGYSP G P
Sbjct: 421 VEKAALASVASLSKNGFERVMIKNKLDAIAAPGGLISPVLAIGGFPGISVPAGYSPDGVP 480

Query: 481 FGIDFGGLKGYEPKLIEIAYGFEQATKRRRKPPL 510
           +GI FGGLKG+EP+LIEIAYGFEQ TK  RKPPL
Sbjct: 481 YGIGFGGLKGFEPRLIEIAYGFEQLTK-SRKPPL 510

BLAST of MS020615 vs. NCBI nr
Match: XP_008457659.1 (PREDICTED: putative amidase C869.01 isoform X1 [Cucumis melo] >KAA0045712.1 putative amidase isoform X1 [Cucumis melo var. makuwa] >TYJ99568.1 putative amidase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 647.9 bits (1670), Expect = 7.3e-182
Identity = 345/515 (66.99%), Postives = 406/515 (78.83%), Query Frame = 0

Query: 1   METPRFSLSFSLFLILVAV-SPFGSYSVR-PAGFPIEEAGLKDLHLAFKQNKLTSRQLVE 60
           M T  F +  S+ L L+A+ S +GS S      F IEEA LKD  LAF QNKLTSRQLVE
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  FYIKQIRRYNPRLRGVIEVNPDALYLADKADRERKAKAPGSLPLLHGIPVLVKDNMATKD 120
           FY++Q+RR NP L+G+IEVNPDAL  A +AD +RK  +  SL  LHGIPVLVKDN+ATKD
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSLALLGSVVPRDAGAVMRLRKAGAIILGKASMSEWAGFRSNGAPTGWNARAGQ 180
           KLNTTAGS ALLGS+VPRDAG V +LRKAGAII GKAS+SEW+GFRS   P GW+AR GQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GREPYTLGLPCGSSSGSAISVSANMAAVTLGTETDGSILCPSSFNSVVGIKPTLGLTSTA 240
           G+ PYT+G PCGSSSGSAISV+ANM  V+LGTETDGSILCPS+ NSVVGIKPT+GLTS A
Sbjct: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 241 GVVPISPRQDTVGPICRTVSDAAYVLDVIVGTDPLDNSTYKASRYIPRGGYGQFLKADGL 300
           GVVPIS RQDTVGPICRTV+DAAYVLD I G D  DNST +AS+YIPRGGYGQFL+A+GL
Sbjct: 241 GVVPISLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGL 300

Query: 301 RGKRLGIVEDF--FGVVDPSLIPAFEEIFTILSKRGAILVDNLKIDNLGAIYNNTESGEV 360
           +GKR+GIV     FG  D   I AFE++F  L + GAILVDNL I+    I   + SGE 
Sbjct: 301 KGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVI-TGSSSGEW 360

Query: 361 AALLNEFKVSLNAYLKELVSSPIRSLAEAIAFNQKHSSLEKIKEYGQDLFLQAEATNGMG 420
            ALL EFK+SLNAYLK+LV+SPIRSL++AI FN+K+S LEK++EYGQ+LFL+AEAT G+G
Sbjct: 361 TALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIG 420

Query: 421 --MNGLLSKLGKLSKDGLEKTMIKNKLDAIMTPSAIISPILAIGGFPGITVPAGYSPSGT 480
                 L++L KLSK+G E+ MIKNKLDAI  P  +ISP LAIGGFPG++VPAGY+P G 
Sbjct: 421 GAEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGL 480

Query: 481 PFGIDFGGLKGYEPKLIEIAYGFEQATKRRRKPPL 510
           PFGI FGGLKG++P+LIEIAYGFE  T  R+ P L
Sbjct: 481 PFGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSL 514

BLAST of MS020615 vs. NCBI nr
Match: XP_004147023.1 (probable amidase At4g34880 [Cucumis sativus] >KGN61907.1 hypothetical protein Csa_006678 [Cucumis sativus])

HSP 1 Score: 647.5 bits (1669), Expect = 9.5e-182
Identity = 345/509 (67.78%), Postives = 407/509 (79.96%), Query Frame = 0

Query: 6   FSLSFSLFLILVAV-SPFGSYSVRPAGFPIEEAGLKDLHLAFKQNKLTSRQLVEFYIKQI 65
           F L  S+ L L+A+ S  GS S       +EEA LKDL  AF QNKLTSRQLVEFY++Q+
Sbjct: 6   FLLDISMLLGLMAILSSCGSCSFH-TELSLEEATLKDLQRAFYQNKLTSRQLVEFYLEQV 65

Query: 66  RRYNPRLRGVIEVNPDALYLADKADRERKAKAPGSLPLLHGIPVLVKDNMATKDKLNTTA 125
           RR NP L+G+IEVNPDAL  A +AD ERK  +P SL  LHGIPVLVKDN+ATKDKLNTTA
Sbjct: 66  RRLNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKLNTTA 125

Query: 126 GSLALLGSVVPRDAGAVMRLRKAGAIILGKASMSEWAGFRSNGAPTGWNARAGQGREPYT 185
           GS ALLGSVVPRDAG V +LR AGAII GKAS+SEW+ FRSN  P+GW+AR GQG+ PYT
Sbjct: 126 GSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNALPSGWSARGGQGKNPYT 185

Query: 186 LGLPCGSSSGSAISVSANMAAVTLGTETDGSILCPSSFNSVVGIKPTLGLTSTAGVVPIS 245
           +G PCGSSSGSAISV+ANM  V+LGTETDGSILCPS+ NSVVGIKPT+GLTS AGVVPIS
Sbjct: 186 MGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPIS 245

Query: 246 PRQDTVGPICRTVSDAAYVLDVIVGTDPLDNSTYKASRYIPRGGYGQFLKADGLRGKRLG 305
            RQDTVGPICRTVSDAAYVL+ IVG D  DNST +AS+YIP+GGYGQFL+A GL+GKR+G
Sbjct: 246 SRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKGKRIG 305

Query: 306 IVEDF--FGVVDPSLIPAFEEIFTILSKRGAILVDNLKIDNLGAIYNNTESGEVAALLNE 365
           IV +F  FG  D     A+E++   L K GAILVDNL IDNL  I++ + SGE  ALL E
Sbjct: 306 IVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILVDNLMIDNLEQIFDGS-SGEQIALLAE 365

Query: 366 FKVSLNAYLKELVSSPIRSLAEAIAFNQKHSSLEKIKEYGQDLFLQAEATNGMG--MNGL 425
           FK+SLNAYLKELV+SPIRSL++AI FN+K+S LE + EYGQ+ FL+AEATNG+G      
Sbjct: 366 FKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENL-EYGQEEFLKAEATNGIGDAEKAA 425

Query: 426 LSKLGKLSKDGLEKTMIKNKLDAIMTPSAIISPILAIGGFPGITVPAGYSPSGTPFGIDF 485
           L++L KLSKDG E+ MIKNKLDA+  P ++ISP+ AIGGFPG++VPAGY P G P+GI F
Sbjct: 426 LARLAKLSKDGFERLMIKNKLDAVAAPGSLISPVFAIGGFPGVSVPAGYDPQGNPYGICF 485

Query: 486 GGLKGYEPKLIEIAYGFEQATKRRRKPPL 510
           GGLKG+EP+LIEIAYGFE+ TK R+ P +
Sbjct: 486 GGLKGFEPRLIEIAYGFERLTKSRKPPSI 511

BLAST of MS020615 vs. NCBI nr
Match: KAF3953674.1 (hypothetical protein CMV_020907 [Castanea mollissima])

HSP 1 Score: 645.6 bits (1664), Expect = 3.6e-181
Identity = 347/507 (68.44%), Postives = 398/507 (78.50%), Query Frame = 0

Query: 7   SLSFSLF--LILVAVSPFGSYSVRPAGFPIEEAGLKDLHLAFKQNKLTSRQLVEFYIKQI 66
           +LSFS+F  LIL+ +    S S     F I+E  + DL LAFKQNKLTSRQLVEFY+K+I
Sbjct: 298 ALSFSIFSSLILILLLTIPSGSCHQINFFIKEVTIHDLQLAFKQNKLTSRQLVEFYLKEI 357

Query: 67  RRYNPRLRGVIEVNPDALYLADKADRERKAKAPGSLPLLHGIPVLVKDNMATKDKLNTTA 126
           RR NP L+GVIEVNPDAL  ADKAD ERKAK PGS   LHGIP+L+KDN+ATKDKLNTTA
Sbjct: 358 RRLNPILKGVIEVNPDALSQADKADYERKAKVPGSQSRLHGIPILLKDNIATKDKLNTTA 417

Query: 127 GSLALLGSVVPRDAGAVMRLRKAGAIILGKASMSEWAGFRSNGAPTGWNARAGQGREPYT 186
           GS ALLGSVVPRDAG V +LRKAGAIILGKAS+ EW+ FR+   P  W+AR GQG+ PYT
Sbjct: 418 GSFALLGSVVPRDAGVVSKLRKAGAIILGKASLGEWSHFRTARGPLAWSARGGQGKNPYT 477

Query: 187 LGLPCGSSSGSAISVSANMAAVTLGTETDGSILCPSSFNSVVGIKPTLGLTSTAGVVPIS 246
           LG PCGSSSGS+ISV+ANM AV+LGTETDGSI+CP+SFNSVVGIKPT+GLTS AGV+PIS
Sbjct: 478 LGEPCGSSSGSSISVAANMVAVSLGTETDGSIICPASFNSVVGIKPTVGLTSRAGVIPIS 537

Query: 247 PRQDTVGPICRTVSDAAYVLDVIVGTDPLDNSTYKASRYIPRGGYGQFLKADGLRGKRLG 306
           PRQDTVGPICRTVSDA +VLD I G D  DN+T+KAS YIP GGYGQFLK DGLRGKRLG
Sbjct: 538 PRQDTVGPICRTVSDAVHVLDAIAGIDNNDNATFKASSYIPSGGYGQFLKVDGLRGKRLG 597

Query: 307 IVED-FFGVVDPS-LIPAFEEIFTILSKRGAILVDNLKIDNLGAIYNNTESGEVAALLNE 366
           IV + FF   + + L   FE+ F  L +RGAILVD+L+I N+G I +   SGE  ALL E
Sbjct: 598 IVRNPFFDFGNNTYLNQTFEQHFDTLRQRGAILVDHLEITNIGMILDYKASGEETALLAE 657

Query: 367 FKVSLNAYLKELVSSPIRSLAEAIAFNQKHSSLEKIKEYGQDLFLQAEATNGMGM--NGL 426
           FK+SLNAYLKELVSSP+R++A+ IAFN K+  LE I E  QD FL AEAT G+G      
Sbjct: 658 FKISLNAYLKELVSSPVRTMADVIAFNNKNPELEMINEIDQDHFLSAEATKGIGKVEKAA 717

Query: 427 LSKLGKLSKDGLEKTMIKNKLDAIMTPSAIISPILAIGGFPGITVPAGYSPSGTPFGIDF 486
           LS L +LSKDG EK M  NKLDA++TPS   S +LAIGGFPG++VPAGY  +G PFGI F
Sbjct: 718 LSNLARLSKDGFEKLMTNNKLDALVTPSYYASSVLAIGGFPGVSVPAGYDTTGEPFGICF 777

Query: 487 GGLKGYEPKLIEIAYGFEQATKRRRKP 508
           GGLKG EPKLIEIAYGFEQA K RR P
Sbjct: 778 GGLKGSEPKLIEIAYGFEQAKKIRRPP 804

BLAST of MS020615 vs. ExPASy Swiss-Prot
Match: A0A1P8B760 (Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 1.5e-153
Identity = 297/508 (58.46%), Postives = 373/508 (73.43%), Query Frame = 0

Query: 10  FSLFLILVAVSPFGSYSVRPAG-------FPIEEAGLKDLHLAFKQNKLTSRQLVEFYIK 69
           FS  LIL+ +S     SV  A        F I+EA ++D+ +AF + +LTS+QLVE Y++
Sbjct: 9   FSSLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLE 68

Query: 70  QIRRYNPRLRGVIEVNPDALYLADKADRERKAKAPGSLPLLHGIPVLVKDNMATKDKLNT 129
            I + NP L  VIE NPDAL  A+ ADRER  K    LP+LHG+PVL+KD+++TKDKLNT
Sbjct: 69  AISKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSISTKDKLNT 128

Query: 130 TAGSLALLGSVVPRDAGAVMRLRKAGAIILGKASMSEWAGFRSNGAPTGWNARAGQGREP 189
           TAGS ALLGSVV RDAG V RLR++GA+ILGKAS+SEWA FRS   P GW+AR  QG+ P
Sbjct: 129 TAGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSARGLQGKNP 188

Query: 190 YTLGL-PCGSSSGSAISVSANMAAVTLGTETDGSILCPSSFNSVVGIKPTLGLTSTAGVV 249
           Y L   P GSSSGSAISV+AN+ AV+LGTETDGSIL P+S NSVVGIKP++GLTS AGVV
Sbjct: 189 YVLSANPSGSSSGSAISVTANLVAVSLGTETDGSILSPASQNSVVGIKPSVGLTSRAGVV 248

Query: 250 PISPRQDTVGPICRTVSDAAYVLDVIVGTDPLDNSTYKASRYIPRGGYGQFLKADGLRGK 309
           PIS RQD++GPICRTVSDA ++LD IVG DPLD +T  AS +IP GGY QFL   GL+GK
Sbjct: 249 PISLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLTTSGLKGK 308

Query: 310 RLGIVEDFFGVVDPSLIPAFEEIFTILSKRGAILVDNLKIDNLGAIYNNTESGEVAALLN 369
           RLGIV     ++D  +          L + GAI+++NL I N+  I   T+SGE  ALL 
Sbjct: 309 RLGIVMKHSSLLDHHI--------KTLRREGAIVINNLTIPNIEVIVGGTDSGEEIALLA 368

Query: 370 EFKVSLNAYLKELVSSPIRSLAEAIAFNQKHSSLEKIKEYGQDLFLQAEATNGMG--MNG 429
           EFK+SLNAYLKELV SP+RSLA+ IA+N++ +  EK+KE+GQ++FL AEAT+GMG     
Sbjct: 369 EFKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKEKT 428

Query: 430 LLSKLGKLSKDGLEKTMIKNKLDAIMTPSAIISPILAIGGFPGITVPAGYSPSGTPFGID 489
            L K+ +LS++G+EK + +NKLDAI+T  + +S +LAIGG+PGI VPAGY   G P+GI 
Sbjct: 429 ALQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYGIS 488

Query: 490 FGGLKGYEPKLIEIAYGFEQATKRRRKP 508
           FGGL+  EPKLIEIA+ FEQAT  R+ P
Sbjct: 489 FGGLRFSEPKLIEIAFAFEQATLIRKPP 508

BLAST of MS020615 vs. ExPASy Swiss-Prot
Match: Q9URY4 (Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC869.01 PE=3 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 2.2e-72
Identity = 196/508 (38.58%), Postives = 276/508 (54.33%), Query Frame = 0

Query: 34  IEEAGLKDLHLAFKQNKLTSRQLVEFYIKQIRRYNPRLRGVIEVNPDALYLADKADRERK 93
           +E+A +  L    +   LTS  +V  Y+ +  + NP + G++++NPD L +A + D ER 
Sbjct: 66  LEDATIDQLQNYMENGILTSTDIVHCYLDRYLQVNPYVNGILQLNPDVLTIASELDDER- 125

Query: 94  AKAPGSL-PLLHGIPVLVKDNMATKDKLNTTAGSLALLGSVVPRDAGAVMRLRKAGAIIL 153
             A G +   LHGIP +VKDN ATKDK++TTAGS ALLGS+VPRDA  V +LR+AGA++ 
Sbjct: 126 --ANGIIRGPLHGIPFIVKDNFATKDKMDTTAGSYALLGSIVPRDAYVVKQLREAGAVLF 185

Query: 154 GKASMSEWAGFRSNGAPTGWNARAGQGREPYTLGL-PCGSSSGSAISVSANMAAVTLGTE 213
           G A++SEWA  RSN    G++AR GQ R P+ L + P GSSSGSAISV++NM A  LGTE
Sbjct: 186 GHATLSEWADMRSNDYSEGYSARGGQSRCPFNLTVNPGGSSSGSAISVASNMIAFALGTE 245

Query: 214 TDGSILCPSSFNSVVGIKPTLGLTSTAGVVPISPRQDTVGPICRTVSDAAYVLDVIVGTD 273
           TDGSI+ P+  N VVG+KPT+GLTS  GV+P S  QDT GPI RTV DA YV   + G D
Sbjct: 246 TDGSIIDPAMRNGVVGLKPTVGLTSRYGVIPESEHQDTTGPIARTVRDAVYVFQSMWGID 305

Query: 274 PLDNSTYKASRYIPR-GGYGQFL-KADGLRGKRLGIV-EDFFGVVDPSLIPAFEEIFTIL 333
             D  T   +   P  G Y +FL     L G R G+  +  +       I    E+   +
Sbjct: 306 ENDIYTLNQTGKTPEDGDYMKFLSNKTSLEGARFGLPWKRLWQNAKADEIDRLLEVVKQI 365

Query: 334 SKRGAILVDNLKIDNLGAIYN---NTESG-----EVAALLNEFKVSLNAYLKELVSSPIR 393
            + GAI+ +N    NL  I N   N E G     E   +  +F  ++ +YL E+ ++ I 
Sbjct: 366 EEAGAIVYNNTNFYNLDVISNDGWNWELGSVNESEYTVVKVDFYNNIKSYLSEVKNTEIH 425

Query: 394 SLAEAIAFNQKHSSLEKIK-------EYGQDLFLQAEATNGMGMN---GLLSKLGKLSKD 453
           SL + + +N K+   E  K         GQD FL +    G+        +  + + S+D
Sbjct: 426 SLEDIVEYNNKYMGTEGGKPNIVPAFSSGQDGFLASLEWGGVKNETYWQAVEYVRRTSQD 485

Query: 454 -GLE--------KTMIKNKLDAIMTPS--AIISPILAIGGFPGITVPAGYSPSGTPFGID 508
            G++        KT     L+ ++ PS  +I     A  G+P IT+P G   +G PFG+ 
Sbjct: 486 EGIDYALNYTDPKTNDSFILNGLLVPSGTSITYQQAAKAGYPMITLPIGVKTNGRPFGLG 545

BLAST of MS020615 vs. ExPASy Swiss-Prot
Match: D4B3C8 (Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_02965 PE=1 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 1.1e-66
Identity = 181/499 (36.27%), Postives = 265/499 (53.11%), Query Frame = 0

Query: 42  LHLAFKQNKLTSRQLVEFYIKQIRRYNPRLRGVIEVNPDALYLADKADRERK-AKAPGSL 101
           L   ++Q  +    +V+ Y+ +I   N  +R V E+NPDAL +A + D ERK  K  G  
Sbjct: 94  LQTPYRQGNVLIIVVVQTYVARIAEVNSTVRAVTEINPDALTIAKQMDNERKMGKLRGP- 153

Query: 102 PLLHGIPVLVKDNMATKDKLNTTAGSLALLGSVVPRDAGAVMRLRKAGAIILGKASMSEW 161
             LHG+P+++K+N+ T DK+++TAGS A+ G+    DA    +LR+AG +I+GK+  S+W
Sbjct: 154 --LHGLPIVIKNNIFTDDKMSSTAGSYAIFGARTSADATVATKLREAGLVIMGKSGASQW 213

Query: 162 AGFRSNGAPTGWNARAGQGREPYTLGL-PCGSSSGSAISVSANMAAVTLGTETDGSILCP 221
           A FRS  +  GW+A  GQ    Y     P GSSSGS ++    +A  TLGTET GSI+ P
Sbjct: 214 ANFRSLNSTNGWSAYGGQVTAAYIKNQDPSGSSSGSGVASDLGLAFATLGTETSGSIVSP 273

Query: 222 SSFNSVVGIKPTLGLTSTAGVVPISPRQDTVGPICRTVSDAAYVLDVIVGTDPLDNSTYK 281
           +  +++VG+KPT+GLTS   VVPIS RQDTVGP+ R+V DAAY+L VI G D  DN T  
Sbjct: 274 ADKSNIVGLKPTVGLTSRRFVVPISERQDTVGPMARSVKDAAYLLQVIAGKDSNDNYT-- 333

Query: 282 ASRYIPRGGYGQFLKA---DGLRGKRLGIVEDFFGVV--DPSLIPAFEEIFTILSKRGAI 341
               IP      ++KA   + L+GKR+G+  +   +     +++  F +   ++ K GAI
Sbjct: 334 --SAIPFDTIPDYVKACDINALKGKRIGVPRNVIKIFGSPQTVVDQFNQALAVMKKAGAI 393

Query: 342 LVDNLKIDNLGAIYNNTESGEVAALLNEFKVSLNAYLKELVSSP--IRSLAEAIAFNQKH 401
           +V+N    +      +    ++  L  +   +L A+ K+L  +P  I  L     F Q H
Sbjct: 394 IVENTDFTSFAEFAQSPIPDDI--LYADSLTNLPAFFKQLKVNPHNITDLESLRRFTQHH 453

Query: 402 SSLEKIKEYGQ------DLFLQAEATNG----MGMNGLLSKLGKLSKDGLEKTMIKNKLD 461
               +++EY        D+ LQ    N       M     K G  ++ G+   + ++KLD
Sbjct: 454 ----RLEEYPSRDTARWDIALQKGIKNTDPKFWPMYQKNVKFG--NEGGILGALRRHKLD 513

Query: 462 AIMTPSAIISPILAIGGFPGITVPAGYSPSGT---------------PFGIDFGGLKGYE 507
           A + P+ +   I A+ G P ITVP G  P+GT               P GI F G    E
Sbjct: 514 AAVLPTDLSPYIPALIGSPIITVPMGVYPNGTKVNHDRELVTSGPGIPIGIGFMGDLWSE 573

BLAST of MS020615 vs. ExPASy Swiss-Prot
Match: B0KBN4 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=gatA PE=3 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 1.5e-41
Identity = 146/500 (29.20%), Postives = 249/500 (49.80%), Query Frame = 0

Query: 39  LKDLHLAFKQNKLTSRQLVEFYIKQIRRYNPRLRGVIEVNPD-ALYLADKADRERKAKAP 98
           + +L    K+ ++++ ++ + Y+++I+   P++  +I +  D AL  A +AD + K    
Sbjct: 8   IHELRELLKKREVSALEVTKSYLERIKEVEPKIDALITITEDFALQRAKEADEKIK---N 67

Query: 99  GSLPLLHGIPVLVKDNMATKDKLNTTAGSLALLGSVVPRDAGAVMRLRKAGAIILGKASM 158
           G    L GIPV++KDN++T + + TT  S  L   + P +A  V +L + G IILGK+++
Sbjct: 68  GEDTALTGIPVIIKDNIST-EGIKTTCSSKMLENYIPPYNATVVEKLLEEGVIILGKSNL 127

Query: 159 SEWAGFRSNGAPTGWNARAGQGREPYTLG-LPCGSSSGSAISVSANMAAVTLGTETDGSI 218
            E+A     G+ T  N+     + P+ L  +P GSS GSA +++A+ AA  LG++T GSI
Sbjct: 128 DEFA----MGSSTE-NSAFKTTKNPWDLSRVPGGSSGGSAAAIAADEAAFALGSDTGGSI 187

Query: 219 LCPSSFNSVVGIKPTLGLTSTAGVVPISPRQDTVGPICRTVSDAAYVLDVIVGTDPLDNS 278
             P+S   VVG+KPT GL S  G+V  +   D +GP  + V+D A VL+ I+G DP D++
Sbjct: 188 RQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTIIGHDPKDST 247

Query: 279 TYKASRYIPRGGYGQFLKADGLRGKRLGIVEDFFGV-VDPSLIPAFEEIFTILSKRGAIL 338
           + K    I +  Y  +LK D ++G R+G+ ++FFG  ++  +    +E   +L   GA +
Sbjct: 248 SLK----IDKPDYTSYLKED-IKGLRIGVAKEFFGEGIEEGVKETVQESIKVLQDLGAEI 307

Query: 339 VD-----------------NLKIDNLGAIYNNTESGEVAA----LLNEFKVSLNAYLKEL 398
           +D                 + +  +  A Y+    G +A     L++ + V+ +    + 
Sbjct: 308 IDISIPYVEYALPAYYIIASAEASSNLARYDGIRYGHIAGKYEDLIDMYMVTRSEGFGKE 367

Query: 399 VSSPIRSLAEAIAFNQKHSSLEK-------IKEYGQDLFLQAEATNGMGMNGLLSKLGKL 458
           V   I     A++     +  +K       IK   +  F + +   G     +  K+G+ 
Sbjct: 368 VKRRIMLGTYALSSGYYDAYYKKALKVRTLIKNDFEKAFEKCDVIIGPTSPTVAFKIGER 427

Query: 459 SKDGLEKTMIKNKLDAIMTPSAIISPILAIGGFPGITVPAGYSPSGTPFGIDFGGLKGYE 508
           + D L            M  + I +  + I G PGI++P G S  G P G+   G    E
Sbjct: 428 ANDPL-----------AMYLADIYTVSVNIAGLPGISIPCGLS-DGLPVGLQIIGRHFDE 481

BLAST of MS020615 vs. ExPASy Swiss-Prot
Match: B0K3S3 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter sp. (strain X514) OX=399726 GN=gatA PE=3 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 2.6e-41
Identity = 145/500 (29.00%), Postives = 249/500 (49.80%), Query Frame = 0

Query: 39  LKDLHLAFKQNKLTSRQLVEFYIKQIRRYNPRLRGVIEVNPD-ALYLADKADRERKAKAP 98
           + +L    K+ ++++ ++ + Y+++I+   P++  ++ +  D AL  A +AD + K    
Sbjct: 8   IHELRELLKKREVSALEVTKSYLERIKEVEPKIDALVTITEDFALQKAKEADEKIK---K 67

Query: 99  GSLPLLHGIPVLVKDNMATKDKLNTTAGSLALLGSVVPRDAGAVMRLRKAGAIILGKASM 158
           G    L GIPV++KDN++T + + TT  S  L   + P +A  V +L + G IILGK+++
Sbjct: 68  GEDTALTGIPVIIKDNIST-EGIKTTCSSKMLENYIPPYNATVVEKLLEEGVIILGKSNL 127

Query: 159 SEWAGFRSNGAPTGWNARAGQGREPYTLG-LPCGSSSGSAISVSANMAAVTLGTETDGSI 218
            E+A     G+ T  N+     + P+ L  +P GSS GSA +++A+ AA  LG++T GSI
Sbjct: 128 DEFA----MGSSTE-NSAFKTTKNPWDLSRVPGGSSGGSAAAIAADEAAFALGSDTGGSI 187

Query: 219 LCPSSFNSVVGIKPTLGLTSTAGVVPISPRQDTVGPICRTVSDAAYVLDVIVGTDPLDNS 278
             P+S   VVG+KPT GL S  G+V  +   D +GP  + V+D A VL+ I+G DP D++
Sbjct: 188 RQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTIIGHDPKDST 247

Query: 279 TYKASRYIPRGGYGQFLKADGLRGKRLGIVEDFFGV-VDPSLIPAFEEIFTILSKRGAIL 338
           + K    I +  Y  +LK D ++G R+G+ ++FFG  ++  +    +E   +L   GA +
Sbjct: 248 SLK----IDKPDYTSYLKED-IKGLRIGVAKEFFGEGIEEGVKETVQESIKVLQDLGAEI 307

Query: 339 VD-----------------NLKIDNLGAIYNNTESGEVAA----LLNEFKVSLNAYLKEL 398
           +D                 + +  +  A Y+    G +A     L++ + V+ +    + 
Sbjct: 308 IDISIPYVEYALPAYYIIASAEASSNLARYDGIRYGHIAEKYEDLIDMYMVTRSEGFGKE 367

Query: 399 VSSPIRSLAEAIAFNQKHSSLEK-------IKEYGQDLFLQAEATNGMGMNGLLSKLGKL 458
           V   I     A++     +  +K       IK   +  F + +   G     +  K+G+ 
Sbjct: 368 VKRRIMLGTYALSSGYYDAYYKKALKVRTLIKNDFEKAFEKCDVIIGPTSPTVAFKIGER 427

Query: 459 SKDGLEKTMIKNKLDAIMTPSAIISPILAIGGFPGITVPAGYSPSGTPFGIDFGGLKGYE 508
           + D L            M  + I +  + I G PGI++P G S  G P G+   G    E
Sbjct: 428 ANDPL-----------AMYLADIYTVSVNIAGLPGISIPCGLS-DGLPVGLQIIGRHFDE 481

BLAST of MS020615 vs. ExPASy TrEMBL
Match: A0A6J1CXI8 (putative amidase C869.01 OS=Momordica charantia OX=3673 GN=LOC111015494 PE=4 SV=1)

HSP 1 Score: 978.0 bits (2527), Expect = 1.5e-281
Identity = 505/509 (99.21%), Postives = 506/509 (99.41%), Query Frame = 0

Query: 1   METPRFSLSFSLFLILVAVSPFGSYSVRPAGFPIEEAGLKDLHLAFKQNKLTSRQLVEFY 60
           METPR SLSFSLFLILVAVSPF SYSVRPAGFPIEEAGLKDLHLAFKQNKLTSRQLVEFY
Sbjct: 1   METPRLSLSFSLFLILVAVSPFWSYSVRPAGFPIEEAGLKDLHLAFKQNKLTSRQLVEFY 60

Query: 61  IKQIRRYNPRLRGVIEVNPDALYLADKADRERKAKAPGSLPLLHGIPVLVKDNMATKDKL 120
           IKQIRRYNPRLRGVIEVNPDALYLADKADRERKAKAPGSLPLLHGIPVLVKDNMATKDKL
Sbjct: 61  IKQIRRYNPRLRGVIEVNPDALYLADKADRERKAKAPGSLPLLHGIPVLVKDNMATKDKL 120

Query: 121 NTTAGSLALLGSVVPRDAGAVMRLRKAGAIILGKASMSEWAGFRSNGAPTGWNARAGQGR 180
           NTTAGSLALLGSVVPRDAGAVMRLRKAGAIILGKASMSEWAGFRSNGAPTGWNARAGQGR
Sbjct: 121 NTTAGSLALLGSVVPRDAGAVMRLRKAGAIILGKASMSEWAGFRSNGAPTGWNARAGQGR 180

Query: 181 EPYTLGLPCGSSSGSAISVSANMAAVTLGTETDGSILCPSSFNSVVGIKPTLGLTSTAGV 240
           EPYTLGLPCGSSSGSAISVSANMAAV LGTETDGSILCPSSFNSVVGIKPTLGLTSTAGV
Sbjct: 181 EPYTLGLPCGSSSGSAISVSANMAAVALGTETDGSILCPSSFNSVVGIKPTLGLTSTAGV 240

Query: 241 VPISPRQDTVGPICRTVSDAAYVLDVIVGTDPLDNSTYKASRYIPRGGYGQFLKADGLRG 300
           VPISPRQDTVGPICRTVSDAAYVLDVIVGTDPLDNSTYKASRYIPRGGYGQFLKADGLRG
Sbjct: 241 VPISPRQDTVGPICRTVSDAAYVLDVIVGTDPLDNSTYKASRYIPRGGYGQFLKADGLRG 300

Query: 301 KRLGIVEDFFGVVDPSLIPAFEEIFTILSKRGAILVDNLKIDNLGAIYNNTESGEVAALL 360
           KRLGIVEDFFGVVDPSLIPAFEEIFTILSKRGAILVDNLKIDNLGAIYNNTESGEVAALL
Sbjct: 301 KRLGIVEDFFGVVDPSLIPAFEEIFTILSKRGAILVDNLKIDNLGAIYNNTESGEVAALL 360

Query: 361 NEFKVSLNAYLKELVSSPIRSLAEAIAFNQKHSSLEKIKEYGQDLFLQAEATNGMGMNGL 420
           NEFKVSLNAYLKELVSSPIRSLAEAIAFNQKHSSLEKIKEYGQDLFLQAEATNGMG+NGL
Sbjct: 361 NEFKVSLNAYLKELVSSPIRSLAEAIAFNQKHSSLEKIKEYGQDLFLQAEATNGMGINGL 420

Query: 421 LSKLGKLSKDGLEKTMIKNKLDAIMTPSAIISPILAIGGFPGITVPAGYSPSGTPFGIDF 480
           LSKLGKLSKDGLEKTMIKNKLDAIMTPSAIISPILAIGGFPGITVPAGYSPSGTPFGIDF
Sbjct: 421 LSKLGKLSKDGLEKTMIKNKLDAIMTPSAIISPILAIGGFPGITVPAGYSPSGTPFGIDF 480

Query: 481 GGLKGYEPKLIEIAYGFEQATKRRRKPPL 510
           GGLKGYEPKLIEIAYGFEQATKRRRKPPL
Sbjct: 481 GGLKGYEPKLIEIAYGFEQATKRRRKPPL 509

BLAST of MS020615 vs. ExPASy TrEMBL
Match: A0A5D3BN38 (Putative amidase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001380 PE=4 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 3.5e-182
Identity = 345/515 (66.99%), Postives = 406/515 (78.83%), Query Frame = 0

Query: 1   METPRFSLSFSLFLILVAV-SPFGSYSVR-PAGFPIEEAGLKDLHLAFKQNKLTSRQLVE 60
           M T  F +  S+ L L+A+ S +GS S      F IEEA LKD  LAF QNKLTSRQLVE
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  FYIKQIRRYNPRLRGVIEVNPDALYLADKADRERKAKAPGSLPLLHGIPVLVKDNMATKD 120
           FY++Q+RR NP L+G+IEVNPDAL  A +AD +RK  +  SL  LHGIPVLVKDN+ATKD
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSLALLGSVVPRDAGAVMRLRKAGAIILGKASMSEWAGFRSNGAPTGWNARAGQ 180
           KLNTTAGS ALLGS+VPRDAG V +LRKAGAII GKAS+SEW+GFRS   P GW+AR GQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GREPYTLGLPCGSSSGSAISVSANMAAVTLGTETDGSILCPSSFNSVVGIKPTLGLTSTA 240
           G+ PYT+G PCGSSSGSAISV+ANM  V+LGTETDGSILCPS+ NSVVGIKPT+GLTS A
Sbjct: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 241 GVVPISPRQDTVGPICRTVSDAAYVLDVIVGTDPLDNSTYKASRYIPRGGYGQFLKADGL 300
           GVVPIS RQDTVGPICRTV+DAAYVLD I G D  DNST +AS+YIPRGGYGQFL+A+GL
Sbjct: 241 GVVPISLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGL 300

Query: 301 RGKRLGIVEDF--FGVVDPSLIPAFEEIFTILSKRGAILVDNLKIDNLGAIYNNTESGEV 360
           +GKR+GIV     FG  D   I AFE++F  L + GAILVDNL I+    I   + SGE 
Sbjct: 301 KGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVI-TGSSSGEW 360

Query: 361 AALLNEFKVSLNAYLKELVSSPIRSLAEAIAFNQKHSSLEKIKEYGQDLFLQAEATNGMG 420
            ALL EFK+SLNAYLK+LV+SPIRSL++AI FN+K+S LEK++EYGQ+LFL+AEAT G+G
Sbjct: 361 TALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIG 420

Query: 421 --MNGLLSKLGKLSKDGLEKTMIKNKLDAIMTPSAIISPILAIGGFPGITVPAGYSPSGT 480
                 L++L KLSK+G E+ MIKNKLDAI  P  +ISP LAIGGFPG++VPAGY+P G 
Sbjct: 421 GAEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGL 480

Query: 481 PFGIDFGGLKGYEPKLIEIAYGFEQATKRRRKPPL 510
           PFGI FGGLKG++P+LIEIAYGFE  T  R+ P L
Sbjct: 481 PFGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSL 514

BLAST of MS020615 vs. ExPASy TrEMBL
Match: A0A1S3C7A8 (putative amidase C869.01 isoform X1 OS=Cucumis melo OX=3656 GN=LOC107990287 PE=4 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 3.5e-182
Identity = 345/515 (66.99%), Postives = 406/515 (78.83%), Query Frame = 0

Query: 1   METPRFSLSFSLFLILVAV-SPFGSYSVR-PAGFPIEEAGLKDLHLAFKQNKLTSRQLVE 60
           M T  F +  S+ L L+A+ S +GS S      F IEEA LKD  LAF QNKLTSRQLVE
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  FYIKQIRRYNPRLRGVIEVNPDALYLADKADRERKAKAPGSLPLLHGIPVLVKDNMATKD 120
           FY++Q+RR NP L+G+IEVNPDAL  A +AD +RK  +  SL  LHGIPVLVKDN+ATKD
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSLALLGSVVPRDAGAVMRLRKAGAIILGKASMSEWAGFRSNGAPTGWNARAGQ 180
           KLNTTAGS ALLGS+VPRDAG V +LRKAGAII GKAS+SEW+GFRS   P GW+AR GQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GREPYTLGLPCGSSSGSAISVSANMAAVTLGTETDGSILCPSSFNSVVGIKPTLGLTSTA 240
           G+ PYT+G PCGSSSGSAISV+ANM  V+LGTETDGSILCPS+ NSVVGIKPT+GLTS A
Sbjct: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 241 GVVPISPRQDTVGPICRTVSDAAYVLDVIVGTDPLDNSTYKASRYIPRGGYGQFLKADGL 300
           GVVPIS RQDTVGPICRTV+DAAYVLD I G D  DNST +AS+YIPRGGYGQFL+A+GL
Sbjct: 241 GVVPISLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGL 300

Query: 301 RGKRLGIVEDF--FGVVDPSLIPAFEEIFTILSKRGAILVDNLKIDNLGAIYNNTESGEV 360
           +GKR+GIV     FG  D   I AFE++F  L + GAILVDNL I+    I   + SGE 
Sbjct: 301 KGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVI-TGSSSGEW 360

Query: 361 AALLNEFKVSLNAYLKELVSSPIRSLAEAIAFNQKHSSLEKIKEYGQDLFLQAEATNGMG 420
            ALL EFK+SLNAYLK+LV+SPIRSL++AI FN+K+S LEK++EYGQ+LFL+AEAT G+G
Sbjct: 361 TALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIG 420

Query: 421 --MNGLLSKLGKLSKDGLEKTMIKNKLDAIMTPSAIISPILAIGGFPGITVPAGYSPSGT 480
                 L++L KLSK+G E+ MIKNKLDAI  P  +ISP LAIGGFPG++VPAGY+P G 
Sbjct: 421 GAEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGL 480

Query: 481 PFGIDFGGLKGYEPKLIEIAYGFEQATKRRRKPPL 510
           PFGI FGGLKG++P+LIEIAYGFE  T  R+ P L
Sbjct: 481 PFGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSL 514

BLAST of MS020615 vs. ExPASy TrEMBL
Match: A0A5B6YIK7 (Amidase domain-containing protein OS=Davidia involucrata OX=16924 GN=Din_000704 PE=4 SV=1)

HSP 1 Score: 647.5 bits (1669), Expect = 4.6e-182
Identity = 345/500 (69.00%), Postives = 405/500 (81.00%), Query Frame = 0

Query: 14  LILVAVSPFGSYSVRPAGFPIEEAGLKDLHLAFKQNKLTSRQLVEFYIKQIRRYNPRLRG 73
           LILVA+S  GS+ ++     I+EA L D+  +FKQN+LTSRQLVEFY++QI R NP LRG
Sbjct: 10  LILVALSLLGSHIIKCHALSIKEATLHDIQTSFKQNQLTSRQLVEFYLEQIHRLNPVLRG 69

Query: 74  VIEVNPDALYLADKADRERKAKAPGSLPLLHGIPVLVKDNMATKDKLNTTAGSLALLGSV 133
           VIEVNPDALY ADKADRERKAKAPGSL  LHGIP+LVKDN+ATKDKLNTTAGS +LLGSV
Sbjct: 70  VIEVNPDALYEADKADRERKAKAPGSLFGLHGIPILVKDNIATKDKLNTTAGSFSLLGSV 129

Query: 134 VPRDAGAVMRLRKAGAIILGKASMSEWAGFRSNGAPTGWNARAGQGREPYTLGL-PCGSS 193
           VPRDAG VM+LRKAGAIILGKAS+SEWA FRS  AP GW+AR G G+ PY L   PCGSS
Sbjct: 130 VPRDAGVVMKLRKAGAIILGKASLSEWANFRSVAAPNGWSARGGHGKNPYVLSADPCGSS 189

Query: 194 SGSAISVSANMAAVTLGTETDGSILCPSSFNSVVGIKPTLGLTSTAGVVPISPRQDTVGP 253
           SGSAISV+ANM AV+LGTETDGSILCP+SFN+VVGIKPT+GLTS AGV+P+SPRQDT+GP
Sbjct: 190 SGSAISVAANMVAVSLGTETDGSILCPASFNAVVGIKPTVGLTSRAGVIPVSPRQDTIGP 249

Query: 254 ICRTVSDAAYVLDVIVGTDPLD-NSTYKASRYIPRGGYGQFLKADGLRGKRLGIV-EDFF 313
           ICRTVSDA YVLD I G D  D  +T +AS+YIPRGGY QFLK DGLRGKRLGI+ + FF
Sbjct: 250 ICRTVSDAVYVLDAIAGFDHNDAEATREASKYIPRGGYAQFLKFDGLRGKRLGIIRKPFF 309

Query: 314 GVVDPSLIP-AFEEIFTILSKRGAILVDNLKIDNLGAIYNNTESGEVAALLNEFKVSLNA 373
              + S++P  FE+ F  L +RGAILVD+L+I N+  I + T SGE  AL+ EFK+SLNA
Sbjct: 310 IFANDSVLPHVFEQHFQTLRQRGAILVDHLEIVNIDMILDFTLSGEATALVAEFKLSLNA 369

Query: 374 YLKELVSSPIRSLAEAIAFNQKHSSLEKIKEYGQDLFLQAEATNGMG--MNGLLSKLGKL 433
           YLK+LV+SP+RSLA+ IAFNQK+S LE I+E+GQ +FL AEATNG+G      L  L +L
Sbjct: 370 YLKDLVASPVRSLADVIAFNQKNSDLEMIEEFGQAIFLAAEATNGIGDAEKKALINLARL 429

Query: 434 SKDGLEKTMIKNKLDAIMTPSAIISPILAIGGFPGITVPAGYSPSGTPFGIDFGGLKGYE 493
           ++DG EK M KNKLDA++TP   ++P+LAIGGFPGI+VPA Y   G P GI FGGLKG E
Sbjct: 430 TQDGFEKVMRKNKLDALVTPGPNVAPVLAIGGFPGISVPAAYDNKGVPVGICFGGLKGSE 489

Query: 494 PKLIEIAYGFEQATKRRRKP 508
           PKLIEIAYGFEQATK R+ P
Sbjct: 490 PKLIEIAYGFEQATKIRKPP 509

BLAST of MS020615 vs. ExPASy TrEMBL
Match: A0A0A0LM97 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G264020 PE=4 SV=1)

HSP 1 Score: 647.5 bits (1669), Expect = 4.6e-182
Identity = 345/509 (67.78%), Postives = 407/509 (79.96%), Query Frame = 0

Query: 6   FSLSFSLFLILVAV-SPFGSYSVRPAGFPIEEAGLKDLHLAFKQNKLTSRQLVEFYIKQI 65
           F L  S+ L L+A+ S  GS S       +EEA LKDL  AF QNKLTSRQLVEFY++Q+
Sbjct: 6   FLLDISMLLGLMAILSSCGSCSFH-TELSLEEATLKDLQRAFYQNKLTSRQLVEFYLEQV 65

Query: 66  RRYNPRLRGVIEVNPDALYLADKADRERKAKAPGSLPLLHGIPVLVKDNMATKDKLNTTA 125
           RR NP L+G+IEVNPDAL  A +AD ERK  +P SL  LHGIPVLVKDN+ATKDKLNTTA
Sbjct: 66  RRLNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKLNTTA 125

Query: 126 GSLALLGSVVPRDAGAVMRLRKAGAIILGKASMSEWAGFRSNGAPTGWNARAGQGREPYT 185
           GS ALLGSVVPRDAG V +LR AGAII GKAS+SEW+ FRSN  P+GW+AR GQG+ PYT
Sbjct: 126 GSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNALPSGWSARGGQGKNPYT 185

Query: 186 LGLPCGSSSGSAISVSANMAAVTLGTETDGSILCPSSFNSVVGIKPTLGLTSTAGVVPIS 245
           +G PCGSSSGSAISV+ANM  V+LGTETDGSILCPS+ NSVVGIKPT+GLTS AGVVPIS
Sbjct: 186 MGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPIS 245

Query: 246 PRQDTVGPICRTVSDAAYVLDVIVGTDPLDNSTYKASRYIPRGGYGQFLKADGLRGKRLG 305
            RQDTVGPICRTVSDAAYVL+ IVG D  DNST +AS+YIP+GGYGQFL+A GL+GKR+G
Sbjct: 246 SRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKGKRIG 305

Query: 306 IVEDF--FGVVDPSLIPAFEEIFTILSKRGAILVDNLKIDNLGAIYNNTESGEVAALLNE 365
           IV +F  FG  D     A+E++   L K GAILVDNL IDNL  I++ + SGE  ALL E
Sbjct: 306 IVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILVDNLMIDNLEQIFDGS-SGEQIALLAE 365

Query: 366 FKVSLNAYLKELVSSPIRSLAEAIAFNQKHSSLEKIKEYGQDLFLQAEATNGMG--MNGL 425
           FK+SLNAYLKELV+SPIRSL++AI FN+K+S LE + EYGQ+ FL+AEATNG+G      
Sbjct: 366 FKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENL-EYGQEEFLKAEATNGIGDAEKAA 425

Query: 426 LSKLGKLSKDGLEKTMIKNKLDAIMTPSAIISPILAIGGFPGITVPAGYSPSGTPFGIDF 485
           L++L KLSKDG E+ MIKNKLDA+  P ++ISP+ AIGGFPG++VPAGY P G P+GI F
Sbjct: 426 LARLAKLSKDGFERLMIKNKLDAVAAPGSLISPVFAIGGFPGVSVPAGYDPQGNPYGICF 485

Query: 486 GGLKGYEPKLIEIAYGFEQATKRRRKPPL 510
           GGLKG+EP+LIEIAYGFE+ TK R+ P +
Sbjct: 486 GGLKGFEPRLIEIAYGFERLTKSRKPPSI 511

BLAST of MS020615 vs. TAIR 10
Match: AT4G34880.1 (Amidase family protein )

HSP 1 Score: 469.9 bits (1208), Expect = 2.5e-132
Identity = 265/507 (52.27%), Postives = 336/507 (66.27%), Query Frame = 0

Query: 10  FSLFLILVAVSPFGSYSVRPAG-------FPIEEAGLKDLHLAFKQNKLTSRQLVEFYIK 69
           FS  LIL+ +S     SV  A        F I+EA ++D+ +AF + +LTS+QLVE Y++
Sbjct: 9   FSSLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLE 68

Query: 70  QIRRYNPRLRGVIEVNPDALYLADKADRERKAKAPGSLPLLHGIPVLVKDNMATKDKLNT 129
            I + NP L  VIE NPDAL  A+ ADRER  K    LP+LHG+PVL+KD+++TKDKLNT
Sbjct: 69  AISKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSISTKDKLNT 128

Query: 130 TAGSLALLGSVVPRDAGAVMRLRKAGAIILGKASMSEWAGFRSNGAPTGWNARAGQGREP 189
           TAGS ALLGSVV RDAG V RLR++GA+ILGKAS+SEWA FRS   P GW+A        
Sbjct: 129 TAGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSA-------- 188

Query: 190 YTLGLPCGSSSGSAISVSANMAAVTLGTETDGSILCPSSFNSVVGIKPTLGLTSTAGVVP 249
                                                 S NSVVGIKP++GLTS AGVVP
Sbjct: 189 -------------------------------------PSQNSVVGIKPSVGLTSRAGVVP 248

Query: 250 ISPRQDTVGPICRTVSDAAYVLDVIVGTDPLDNSTYKASRYIPRGGYGQFLKADGLRGKR 309
           IS RQD++GPICRTVSDA ++LD IVG DPLD +T  AS +IP GGY QFL   GL+GKR
Sbjct: 249 ISLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLTTSGLKGKR 308

Query: 310 LGIVEDFFGVVDPSLIPAFEEIFTILSKRGAILVDNLKIDNLGAIYNNTESGEVAALLNE 369
           LGIV     ++D  +          L + GAI+++NL I N+  I   T+SGE  ALL E
Sbjct: 309 LGIVMKHSSLLDHHI--------KTLRREGAIVINNLTIPNIEVIVGGTDSGEEIALLAE 368

Query: 370 FKVSLNAYLKELVSSPIRSLAEAIAFNQKHSSLEKIKEYGQDLFLQAEATNGMG--MNGL 429
           FK+SLNAYLKELV SP+RSLA+ IA+N++ +  EK+KE+GQ++FL AEAT+GMG      
Sbjct: 369 FKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKEKTA 428

Query: 430 LSKLGKLSKDGLEKTMIKNKLDAIMTPSAIISPILAIGGFPGITVPAGYSPSGTPFGIDF 489
           L K+ +LS++G+EK + +NKLDAI+T  + +S +LAIGG+PGI VPAGY   G P+GI F
Sbjct: 429 LQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYGISF 462

Query: 490 GGLKGYEPKLIEIAYGFEQATKRRRKP 508
           GGL+  EPKLIEIA+ FEQAT  R+ P
Sbjct: 489 GGLRFSEPKLIEIAFAFEQATLIRKPP 462

BLAST of MS020615 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 119.8 bits (299), Expect = 6.4e-27
Identity = 131/479 (27.35%), Postives = 218/479 (45.51%), Query Frame = 0

Query: 52  TSRQLVEFYIKQIRRYNPRLRGVIEVNPDALYLADKADRERKAKAPGSLPLLHGIPVLVK 111
           T+ ++ + Y+ +IR   P+L+  + V+ + L  A + D +R AK     PL  G+ + VK
Sbjct: 59  TAVEIAKSYLSRIRLTEPQLKCFLHVSENVLKDAQEID-QRIAKGEELGPLA-GVLIGVK 118

Query: 112 DNMATKDKLNTTAGSLALLGSVVPRDAGAVMRLRKAGAIILGKASMSEWAGFRSNGAPTG 171
           DN+ T+  + +TA S  L     P DA AV ++++ G I++GK +M E+ G  S    + 
Sbjct: 119 DNICTQG-MPSTAASRILEHYRPPFDATAVKKIKELGGIVVGKTNMDEF-GMGSTTEASA 178

Query: 172 WNARAGQGREPYTLG-LPCGSSSGSAISVSANMAAVTLGTETDGSILCPSSFNSVVGIKP 231
           +   A     P+ L  +P GSS GSA +V+A    V+LG++T GS+  P+SF  VVG+KP
Sbjct: 179 FQVTA----NPWDLSRVPGGSSGGSAAAVAARQCMVSLGSDTGGSVRQPASFCGVVGLKP 238

Query: 232 TLGLTSTAGVVPISPRQDTVGPICRTVSDAAYVLDVIVGTDPLDNSTYKASRYIPRGGYG 291
           T G  S  G++  +   D +G    TV+DA  +L  I G D  D+++ K    +P     
Sbjct: 239 TYGRVSRFGLMAYASSLDVIGCFGSTVADAGMLLHAISGYDRFDSTSSKQD--VPE-FQS 298

Query: 292 QFLKAD-----GLRGKRLGIV-EDFFGVVDPSLIPAFEEIFTILSKRGAILVD-NLKIDN 351
           QFL  D      L G ++GI+ E     VD  +  A +E  + L   G IL + +L   +
Sbjct: 299 QFLSVDHFESKPLNGVKVGIIRETLEDGVDSGVRSATQEAASHLEALGCILTEVSLPSFS 358

Query: 352 LGA----IYNNTESGEVAALLNEFKVSLNAYLKEL-------------VSSPIRSLAEAI 411
           LG     +  ++ES    +  +  +       +EL                 +R L    
Sbjct: 359 LGLPAYYVIASSESSSNLSRYDGVRYGNQVMAEELNKLYECSRGEGFGGEVKMRILMGTY 418

Query: 412 AFNQKHSSLEKIKEYGQDLFLQAEATNGMGMNGLLSKLGKLSKDGLEKTMIKNKLDAIMT 471
           A +  +      +       ++ +    +  N +L  +   +     K   K      M 
Sbjct: 419 ALSAGYYDAYYKRAQQVRTLIRKDFKAALEQNDIL--ISPAAPSAAYKIGEKKDDPLAMY 478

Query: 472 PSAIISPILAIGGFPGITVPAGY---SPSGTPFGIDFGGLKGYEPKLIEIAYGFEQATK 503
              I++  + + G P + +P G     PSG P G+   G    E KL+++ + FEQ  K
Sbjct: 479 AGDIMTVNVNLAGLPAMVLPCGLVEGGPSGLPVGLQMIGAAFDEEKLLKVGHIFEQTLK 524

BLAST of MS020615 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 101.7 bits (252), Expect = 1.8e-21
Identity = 78/238 (32.77%), Postives = 117/238 (49.16%), Query Frame = 0

Query: 47  KQNKLTSRQLVEFYIKQIRRYNPRLRGVIEVNPDALYLADKADRERKAKAPGSLPLLHGI 106
           K  ++TS++LV  Y+KQ++RYN  L  V+    +  Y   K   +  ++     P LHGI
Sbjct: 213 KTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEADDLLSQGTYLGP-LHGI 272

Query: 107 PVLVKDNMATKDKLNTTAGSLALLGSVVPRDAGAVMRLRKAGAIILGK---ASMSE---W 166
           P  +KD +A      TT GS +     +  +A    RL+ +GA+++ K    SM+    W
Sbjct: 273 PYGLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTGSMAYDDIW 332

Query: 167 AGFRSNGAPTGWNARAGQGREPYTL-GLPCGSSSGSAISVSANMAAVTLGTETDGSILCP 226
            G              G+ R P+ +     GSS+G A S SA       G+ET GS+  P
Sbjct: 333 FG--------------GRTRNPWNIEEFSTGSSAGPAASTSA-------GSETAGSMTYP 392

Query: 227 SSFNSVVGIKPTLGLTSTAGVVPISPRQDTVGPICRTVSDAAYVLDVIVGTDPLDNST 278
           ++   +  ++PT G     GV+ IS   D +GP CRT +D A +LD I G DP D S+
Sbjct: 393 AARCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLSS 427

BLAST of MS020615 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 94.7 bits (234), Expect = 2.2e-19
Identity = 113/493 (22.92%), Postives = 202/493 (40.97%), Query Frame = 0

Query: 39  LKDLHLAFKQNKLTSRQLVEFYIKQIRRY---NPRLRGVIEVNPDALYLADKADRERKAK 98
           ++D   A++    T  Q+ +  I  I  +    P    +I  + + +    +A   R+ +
Sbjct: 131 IRDYAYAYRSKLTTPLQVAKRIISIIEEFGYDKPPTPFLIRFDANEVIKQAEAS-TRRFE 190

Query: 99  APGSLPLLHGIPVLVKDNMATKDKLNTTAGSLALLGSVVPRDAGAVMRLRKAGAIILGKA 158
               + +L GI V +KD++           +       V +D+  V +LR  GAI+LGKA
Sbjct: 191 QGNPISVLDGIFVTIKDDIDCLPHPTNGGTTWLHEDRSVEKDSAVVSKLRSCGAILLGKA 250

Query: 159 SMSEWAGFRSNGAPTGWNARAGQGREPY-TLGLPCGSSSGSAISVSANMAAVTLGTETDG 218
           +M E          TG N+  G  R P+       GSSSGSA  V+A + +  LGT+  G
Sbjct: 251 NMHE-----LGMGTTGNNSNYGTTRNPHDPKRYTGGSSSGSAAIVAAGLCSAALGTDGGG 310

Query: 219 SILCPSSFNSVVGIKPTLGLTSTAGVVPISPRQDTVGPICRTVSDAAYVLDVIVGTDPLD 278
           S+  PS+   + G+K T G T   G +      + +GP+  ++ DA  V   I+G+   D
Sbjct: 311 SVRIPSALCGITGLKTTYGRTDMTGSLCEGGTVEIIGPLASSLEDAFLVYAAILGSSSAD 370

Query: 279 NSTYKAS-----RYIPRGGYGQFLKADGLRGKRLGIVEDFFGVVDPS-LIPAFEEIFTIL 338
               K S     + +   G      ++ +   RLG    +F  V  S +    E+I  +L
Sbjct: 371 RYNLKPSPPCFPKLLSHNG------SNAIGSLRLGKYTKWFNDVSSSDISDKCEDILKLL 430

Query: 339 SKRGAILVDNLKIDNLGAIYNNTESGEVAALLNEFKVSLNAYLKELVSSPIR-SLAEAIA 398
           S      V  + +  L        +  V ++ +    SL  Y +   +S +      + A
Sbjct: 431 SNNHGCKVVEIVVPEL----EEMRAAHVISIGSPTLSSLTPYCEAGKNSKLSYDTRTSFA 490

Query: 399 FNQKHSSLE---------KIKEYGQDLFLQAEATNGMGMNGLLSKLGKLSKDGLEKTMIK 458
             +  S+ +         ++ EY  ++F   +         +++    ++   +    +K
Sbjct: 491 IFRSFSASDYIAAQCLRRRLMEYHLNIFKDVDV--------IVTPTTGMTAPVIPPDALK 550

Query: 459 NKLDAIMTPSAIISPILAIG--GFPGITVPAGYSPSGTPFGIDFGGLKGYEPKLIEIAYG 510
           N    I   + ++  +LA    GFP I+VP GY   G P G+   G    E  ++ +A  
Sbjct: 551 NGETNIQVTTDLMRFVLAANLLGFPAISVPVGYDKEGLPIGLQIMGRPWAEATVLGLAAA 599

BLAST of MS020615 vs. TAIR 10
Match: AT1G08980.1 (amidase 1 )

HSP 1 Score: 70.9 bits (172), Expect = 3.4e-12
Identity = 57/160 (35.62%), Postives = 82/160 (51.25%), Query Frame = 0

Query: 99  SLPLLHGIPVLVKDNMATKDKLNTTAGSLALL--GSVVPRDAGAVMRLRKAGAIILGKAS 158
           S P L G+   +KD    + ++ T  G+   L   S     A  V  L +AGA  LG   
Sbjct: 24  SPPSLQGLTFAIKDIFDVEGRV-TGFGNPDWLRTHSAATSTAPVVSSLLEAGATALGITI 83

Query: 159 MSEWAGFRSNGAPTGWNARAGQGREPYTLG-LPCGSSSGSAISVSANMAAVTLGTETDGS 218
           M E A +  NG     NA  G  R P     +P GSSSGSA++V+A +   ++GT+T GS
Sbjct: 84  MDEMA-YSINGE----NAHYGTPRNPIAFDRVPGGSSSGSAVAVAARLVDFSIGTDTGGS 143

Query: 219 ILCPSSFNSVVGIKPTLGLTSTAGVVPISPRQDTVGPICR 256
           +  P+S+  + G +P+ G  ST G+ P++   DTVG   R
Sbjct: 144 VRVPASYCGIFGFRPSHGAVSTVGLTPMAQSFDTVGWFAR 177

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022146229.13.1e-28199.21putative amidase C869.01 [Momordica charantia][more]
XP_038900913.15.1e-18367.90probable amidase At4g34880 [Benincasa hispida][more]
XP_008457659.17.3e-18266.99PREDICTED: putative amidase C869.01 isoform X1 [Cucumis melo] >KAA0045712.1 puta... [more]
XP_004147023.19.5e-18267.78probable amidase At4g34880 [Cucumis sativus] >KGN61907.1 hypothetical protein Cs... [more]
KAF3953674.13.6e-18168.44hypothetical protein CMV_020907 [Castanea mollissima][more]
Match NameE-valueIdentityDescription
A0A1P8B7601.5e-15358.46Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=... [more]
Q9URY42.2e-7238.58Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) ... [more]
D4B3C81.1e-6636.27Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS ... [more]
B0KBN41.5e-4129.20Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter pseudethanol... [more]
B0K3S32.6e-4129.00Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter sp. (strain ... [more]
Match NameE-valueIdentityDescription
A0A6J1CXI81.5e-28199.21putative amidase C869.01 OS=Momordica charantia OX=3673 GN=LOC111015494 PE=4 SV=... [more]
A0A5D3BN383.5e-18266.99Putative amidase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3C7A83.5e-18266.99putative amidase C869.01 isoform X1 OS=Cucumis melo OX=3656 GN=LOC107990287 PE=4... [more]
A0A5B6YIK74.6e-18269.00Amidase domain-containing protein OS=Davidia involucrata OX=16924 GN=Din_000704 ... [more]
A0A0A0LM974.6e-18267.78Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G264020 PE=... [more]
Match NameE-valueIdentityDescription
AT4G34880.12.5e-13252.27Amidase family protein [more]
AT3G25660.16.4e-2727.35Amidase family protein [more]
AT5G07360.21.8e-2132.77Amidase family protein [more]
AT5G64440.12.2e-1922.92fatty acid amide hydrolase [more]
AT1G08980.13.4e-1235.63amidase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023631Amidase signature domainPFAMPF01425Amidasecoord: 56..453
e-value: 4.5E-67
score: 227.0
IPR036928Amidase signature (AS) superfamilyGENE3D3.90.1300.10Amidase signature (AS) domaincoord: 19..509
e-value: 1.9E-143
score: 481.1
IPR036928Amidase signature (AS) superfamilySUPERFAMILY75304Amidase signature (AS) enzymescoord: 34..508
NoneNo IPR availablePANTHERPTHR42678AMIDASEcoord: 28..508
NoneNo IPR availablePANTHERPTHR42678:SF19C869.01-LIKE PROTEIN, PUTATIVE-RELATEDcoord: 28..508

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS020615.1MS020615.1mRNA