MS020574 (gene) Bitter gourd (TR) v1

BLAST of MS020574 vs. NCBI nr
Match: XP_022146234.1 (protein DETOXIFICATION 46, chloroplastic [Momordica charantia])

HSP 1 Score: 1060.1 bits (2740), Expect = 6.7e-306
Identity = 546/550 (99.27%), Postives = 548/550 (99.64%), Query Frame = 0

Query: 1   MPFKILHRPSSITAQIQNHNPKIPIPLARPSFPFTYQSLSCPAIRLRSSFSTSSIDVPSR 60
           MPFKILHRPSSITAQIQN NPKIPIPLARPSFPFTYQSLSCPAIRLRSSFSTSSIDVPSR
Sbjct: 1   MPFKILHRPSSITAQIQNQNPKIPIPLARPSFPFTYQSLSCPAIRLRSSFSTSSIDVPSR 60

Query: 61  VSRRFVVTGDGREIESEIAVEVQENEQLLGSSNREELGSQGLWRQMKEIVTFTGPAIGLW 120
           VSRRFVVTGDGREIESEIAVEVQENEQLLGSSNREELGSQGLWRQMKEIVTFTGPAIGLW
Sbjct: 61  VSRRFVVTGDGREIESEIAVEVQENEQLLGSSNREELGSQGLWRQMKEIVTFTGPAIGLW 120

Query: 121 ICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATALAKQDKD 180
           ICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATALAKQDKD
Sbjct: 121 ICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATALAKQDKD 180

Query: 181 EVQHHISVLLFVGLISGFLMLLATKLLGSVALTAFAGTKNADVIPAANTYVQIRGLAWPA 240
           EVQHHISVLLFVGLISGFLMLLATKLLGSVALTAFAGTKNAD+IPAANTYVQIRGLAWPA
Sbjct: 181 EVQHHISVLLFVGLISGFLMLLATKLLGSVALTAFAGTKNADIIPAANTYVQIRGLAWPA 240

Query: 241 ILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATMASQVIA 300
           ILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATMASQVIA
Sbjct: 241 ILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATMASQVIA 300

Query: 301 AYMMIETLNKKGYRGYSLSVPSPGEFLSILGLAAPVFITMMSKVVFYSLLIYYATSMGTH 360
           AYMMIETLNKKGY GYSLSVPSPGEFLSILGLAAPVFITMMSKVVFYSLLIYYATSMGTH
Sbjct: 301 AYMMIETLNKKGYHGYSLSVPSPGEFLSILGLAAPVFITMMSKVVFYSLLIYYATSMGTH 360

Query: 361 TMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVNPSLDKARMLLKSLLIIGAIFGL 420
           TMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLI+GVNPSLDKARMLLKSLLIIGAIFGL
Sbjct: 361 TMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLIHGVNPSLDKARMLLKSLLIIGAIFGL 420

Query: 421 VLGTIGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVITPPTHSLEGTLLAGRDLKFI 480
           VLGTIGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVITPPTHSLEGTLLAGRDLKFI
Sbjct: 421 VLGTIGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVITPPTHSLEGTLLAGRDLKFI 480

Query: 481 SLSMTGCLCLGAIVLLAICSRGYGLAGCWYALVGFQWARFIIALRRVLSPNGVLYSSDLS 540
           SLSMTGCLCLGAIVLLAICSRGYGLAGCWYALVGFQWARFIIALRRVLSPNGVLYSSDLS
Sbjct: 481 SLSMTGCLCLGAIVLLAICSRGYGLAGCWYALVGFQWARFIIALRRVLSPNGVLYSSDLS 540

Query: 541 HYKLEKQKAA 551
           HYKLEKQKAA
Sbjct: 541 HYKLEKQKAA 550

BLAST of MS020574 vs. NCBI nr
Match: XP_038902802.1 (protein DETOXIFICATION 46, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 884.4 bits (2284), Expect = 5.0e-253
Identity = 470/550 (85.45%), Postives = 491/550 (89.27%), Query Frame = 0

Query: 1   MPFKILHRPSSITAQIQNHNPKIPIPLARPSFPFTYQSLSCPAIRLRSSFSTSSIDVPSR 60
           MPFKI H  SS   +I  H P I  P +  SFP T+QSLS P I   S  S   I   S 
Sbjct: 1   MPFKIFHCSSSPIPRI--HYPNILRPFSPSSFPLTHQSLSSPTISFPSCSSPFPIHFSSP 60

Query: 61  VSRRFVVTGDGREIESEIAVEVQENEQLLGSSNREELGSQGLWRQMKEIVTFTGPAIGLW 120
           + RR  V     +IESEI +EVQENEQLLG+   EELG+QGL  QMKEIVTFTGPAIGLW
Sbjct: 61  LRRRLPV--PRVDIESEIGIEVQENEQLLGTGG-EELGNQGLLSQMKEIVTFTGPAIGLW 120

Query: 121 ICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATALAKQDKD 180
           ICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATALAKQDK+
Sbjct: 121 ICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATALAKQDKN 180

Query: 181 EVQHHISVLLFVGLISGFLMLLATKLLGSVALTAFAGTKNADVIPAANTYVQIRGLAWPA 240
           EVQHHISVLLFVGL+SGFLMLL TKLLGS +LTAF GTKNAD+IPAANTY+QIRGLAWPA
Sbjct: 181 EVQHHISVLLFVGLMSGFLMLLITKLLGSASLTAFVGTKNADIIPAANTYIQIRGLAWPA 240

Query: 241 ILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATMASQVIA 300
           ILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATMASQVIA
Sbjct: 241 ILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATMASQVIA 300

Query: 301 AYMMIETLNKKGYRGYSLSVPSPGEFLSILGLAAPVFITMMSKVVFYSLLIYYATSMGTH 360
           AYMMIETLNKKGY GYSL VPS GEFLSILGLAAPVF+TMMSKVVFYSLLIYYATSMGTH
Sbjct: 301 AYMMIETLNKKGYNGYSLLVPSSGEFLSILGLAAPVFLTMMSKVVFYSLLIYYATSMGTH 360

Query: 361 TMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVNPSLDKARMLLKSLLIIGAIFGL 420
           TMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVN SLDKA MLLKSLLIIGAIFGL
Sbjct: 361 TMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVNRSLDKAWMLLKSLLIIGAIFGL 420

Query: 421 VLGTIGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVITPPTHSLEGTLLAGRDLKFI 480
           VLGTIGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVITPPTHSLEGTLLAGRDLK+I
Sbjct: 421 VLGTIGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVITPPTHSLEGTLLAGRDLKYI 480

Query: 481 SLSMTGCLCLGAIVLLAICSRGYGLAGCWYALVGFQWARFIIALRRVLSPNGVLYSSDLS 540
           SLSMT CL LGA+VLL I SRGYGL GCWYALVGFQWARF+ ALRR+LSP+G+L+SSDLS
Sbjct: 481 SLSMTACLSLGALVLLVISSRGYGLTGCWYALVGFQWARFLTALRRILSPDGILHSSDLS 540

Query: 541 HYKLEKQKAA 551
            YKLEKQKAA
Sbjct: 541 QYKLEKQKAA 545

BLAST of MS020574 vs. NCBI nr
Match: XP_008461005.1 (PREDICTED: protein DETOXIFICATION 46, chloroplastic [Cucumis melo])

HSP 1 Score: 884.0 bits (2283), Expect = 6.5e-253
Identity = 472/556 (84.89%), Postives = 496/556 (89.21%), Query Frame = 0

Query: 1   MPFKILHRPSSITAQIQNHNPKIPIPLARPSFPFTYQSLSCPAIRLRSSFSTSSIDVP-- 60
           MPFKILH  SS +   + H P I  P +  S PFT+ SLS P I    +F +SSI  P  
Sbjct: 1   MPFKILHSSSSSSLVPRFHCPSILKPFSPSSLPFTHPSLSSPTI----TFPSSSISSPLP 60

Query: 61  ----SRVSRRFVVTGDGREIESEIAVEVQENEQLLGSSNREELGSQGLWRQMKEIVTFTG 120
               SR  RRF +     EIE EI +EVQ +EQ+L     EELG+QGL  QMKEIVTFTG
Sbjct: 61  PHFSSRNRRRFPIL--RVEIEREIGIEVQRDEQVL-EIEGEELGNQGLLNQMKEIVTFTG 120

Query: 121 PAIGLWICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATAL 180
           PAIGLWICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATAL
Sbjct: 121 PAIGLWICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATAL 180

Query: 181 AKQDKDEVQHHISVLLFVGLISGFLMLLATKLLGSVALTAFAGTKNADVIPAANTYVQIR 240
           AKQDK+EVQHHISVLLFVGL++GFLMLL+TKLLGSVALTAF GTKNAD+IPAANTY+QIR
Sbjct: 181 AKQDKNEVQHHISVLLFVGLVAGFLMLLSTKLLGSVALTAFVGTKNADIIPAANTYIQIR 240

Query: 241 GLAWPAILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATM 300
           GLAWPAILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATM
Sbjct: 241 GLAWPAILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATM 300

Query: 301 ASQVIAAYMMIETLNKKGYRGYSLSVPSPGEFLSILGLAAPVFITMMSKVVFYSLLIYYA 360
           ASQVIAAYMMIETLNKKGY GYSLSVPS GEFLSILGLAAPVF+TMMSKVVFYSLLIYYA
Sbjct: 301 ASQVIAAYMMIETLNKKGYNGYSLSVPSSGEFLSILGLAAPVFLTMMSKVVFYSLLIYYA 360

Query: 361 TSMGTHTMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVNPSLDKARMLLKSLLII 420
           TSMGTHTMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVN SLDKA MLLKSLLII
Sbjct: 361 TSMGTHTMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVNRSLDKAWMLLKSLLII 420

Query: 421 GAIFGLVLGTIGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVITPPTHSLEGTLLAG 480
           GAIFGLVLGTIGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVITPPTHSLEGTLLAG
Sbjct: 421 GAIFGLVLGTIGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVITPPTHSLEGTLLAG 480

Query: 481 RDLKFISLSMTGCLCLGAIVLLAICSRGYGLAGCWYALVGFQWARFIIALRRVLSPNGVL 540
           RDLK+ISLSMTGCL LGA+VLL I SRGYGL GCWYALVGFQWARF+ ALRR+LSP+GVL
Sbjct: 481 RDLKYISLSMTGCLSLGALVLLIISSRGYGLTGCWYALVGFQWARFLSALRRILSPDGVL 540

Query: 541 YSSDLSHYKLEKQKAA 551
           YSSDLSH +LEKQKAA
Sbjct: 541 YSSDLSHNELEKQKAA 549

BLAST of MS020574 vs. NCBI nr
Match: XP_022986494.1 (protein DETOXIFICATION 46, chloroplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 882.9 bits (2280), Expect = 1.5e-252
Identity = 467/553 (84.45%), Postives = 490/553 (88.61%), Query Frame = 0

Query: 5   ILHRPSSITAQIQNHNPKIPIPLARPSFPFTYQSLSCPAIRLRSSFSTSSIDVPSRVSRR 64
           ILH P S+TA+I  HNP    PL+ PSF         P I  RSS S  +ID+ SR++RR
Sbjct: 5   ILHSPFSVTARI--HNPNNLRPLSPPSF---------PTIFFRSSSSPLAIDLSSRINRR 64

Query: 65  FVVTGDGR-------EIESEIAVEVQENEQLLGSSNREELGSQGLWRQMKEIVTFTGPAI 124
           F V            EIESEI +EVQENEQLLGS   EELGSQGL  QMKEIVTFTGPAI
Sbjct: 65  FAVPRRRHEWEVGCLEIESEIGIEVQENEQLLGSGGGEELGSQGLLTQMKEIVTFTGPAI 124

Query: 125 GLWICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATALAKQ 184
           GLWICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATALAKQ
Sbjct: 125 GLWICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATALAKQ 184

Query: 185 DKDEVQHHISVLLFVGLISGFLMLLATKLLGSVALTAFAGTKNADVIPAANTYVQIRGLA 244
           DK+EVQHHISVLLFVGLISGFLMLL TKLLGSVALTAF GTKNAD+IPAANTY+QIRGLA
Sbjct: 185 DKNEVQHHISVLLFVGLISGFLMLLVTKLLGSVALTAFVGTKNADIIPAANTYIQIRGLA 244

Query: 245 WPAILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATMASQ 304
           WPAILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATM SQ
Sbjct: 245 WPAILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATMVSQ 304

Query: 305 VIAAYMMIETLNKKGYRGYSLSVPSPGEFLSILGLAAPVFITMMSKVVFYSLLIYYATSM 364
           VIAAYMMIE LNKK Y G++LSVPS GEF SILGLAAPVF+ MMSKVVFYSLLIYYATSM
Sbjct: 305 VIAAYMMIEALNKKAYNGFALSVPSSGEFFSILGLAAPVFLPMMSKVVFYSLLIYYATSM 364

Query: 365 GTHTMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVNPSLDKARMLLKSLLIIGAI 424
           GTH MAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVN +LDKARMLLKSLLIIGAI
Sbjct: 365 GTHIMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVNRNLDKARMLLKSLLIIGAI 424

Query: 425 FGLVLGTIGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVITPPTHSLEGTLLAGRDL 484
           FGL+LG+IGT VPWLFP LFTPEE+IIQEMHKVLIPYFLALVITPPTHSLEGTLLAGRDL
Sbjct: 425 FGLILGSIGTLVPWLFPYLFTPEERIIQEMHKVLIPYFLALVITPPTHSLEGTLLAGRDL 484

Query: 485 KFISLSMTGCLCLGAIVLLAICSRGYGLAGCWYALVGFQWARFIIALRRVLSPNGVLYSS 544
           KFISLSMTGCL LGA+VLL I SRGYGL GCWYAL GFQWARF+IALRR+LSP+G+LYSS
Sbjct: 485 KFISLSMTGCLSLGALVLLVISSRGYGLTGCWYALTGFQWARFLIALRRILSPDGMLYSS 544

Query: 545 DLSHYKLEKQKAA 551
           DLSHYKLEK KAA
Sbjct: 545 DLSHYKLEKPKAA 546

BLAST of MS020574 vs. NCBI nr
Match: XP_023512558.1 (protein DETOXIFICATION 46, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 881.7 bits (2277), Expect = 3.2e-252
Identity = 468/554 (84.48%), Postives = 491/554 (88.63%), Query Frame = 0

Query: 5   ILHRPSSITAQIQNHNPKIPIPLARPSFPFTYQSLSCPAIRLRSSFSTSSIDVPSRVSRR 64
           ILH P S+TA+I  HNP I  PL+ PSF         P I  RSS S  +ID+ SR++RR
Sbjct: 5   ILHSPFSVTARI--HNPNIFRPLSPPSF---------PTISFRSSSSPLAIDLSSRINRR 64

Query: 65  FVVTGDGR-------EIESEIAVEVQENEQLLGS-SNREELGSQGLWRQMKEIVTFTGPA 124
           F V            EIESEI +EVQENEQLLGS    EELGS GL  QMKEIVTFTGPA
Sbjct: 65  FAVPRRRHEWEVGCLEIESEIGIEVQENEQLLGSGGGGEELGSPGLLTQMKEIVTFTGPA 124

Query: 125 IGLWICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATALAK 184
           IGLWICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATALAK
Sbjct: 125 IGLWICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATALAK 184

Query: 185 QDKDEVQHHISVLLFVGLISGFLMLLATKLLGSVALTAFAGTKNADVIPAANTYVQIRGL 244
           QDK+EVQHHISVLLFVGL SGFLMLL TKLLGSVALTAF GTKNAD+IPAANTY+QIRGL
Sbjct: 185 QDKNEVQHHISVLLFVGLTSGFLMLLVTKLLGSVALTAFVGTKNADIIPAANTYIQIRGL 244

Query: 245 AWPAILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATMAS 304
           AWPAILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATM S
Sbjct: 245 AWPAILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATMVS 304

Query: 305 QVIAAYMMIETLNKKGYRGYSLSVPSPGEFLSILGLAAPVFITMMSKVVFYSLLIYYATS 364
           QVIAAYMMIE LNKK Y G++LSVPS GEF SILGLAAPVF+TMMSKVVFYSLLIYYATS
Sbjct: 305 QVIAAYMMIEALNKKAYNGFALSVPSSGEFFSILGLAAPVFLTMMSKVVFYSLLIYYATS 364

Query: 365 MGTHTMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVNPSLDKARMLLKSLLIIGA 424
           MGTHTMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVN SLDKARMLLKSLLIIGA
Sbjct: 365 MGTHTMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVNRSLDKARMLLKSLLIIGA 424

Query: 425 IFGLVLGTIGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVITPPTHSLEGTLLAGRD 484
           IFGL+LG+IGT VPWLFP LFTPEE+IIQEMHKVLIPYFLALVITPPTHSLEGTLLAGRD
Sbjct: 425 IFGLILGSIGTLVPWLFPYLFTPEERIIQEMHKVLIPYFLALVITPPTHSLEGTLLAGRD 484

Query: 485 LKFISLSMTGCLCLGAIVLLAICSRGYGLAGCWYALVGFQWARFIIALRRVLSPNGVLYS 544
           LKFISLSMTGCL LGA+VLL I SRGYGL GCWYAL GFQWARF+IALRR+LSP+G+LYS
Sbjct: 485 LKFISLSMTGCLSLGALVLLVISSRGYGLTGCWYALTGFQWARFLIALRRILSPDGMLYS 544

Query: 545 SDLSHYKLEKQKAA 551
           SDLSHYKLE+ KAA
Sbjct: 545 SDLSHYKLERPKAA 547

BLAST of MS020574 vs. ExPASy Swiss-Prot
Match: Q8W4G3 (Protein DETOXIFICATION 46, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DTX46 PE=2 SV=1)

HSP 1 Score: 673.3 bits (1736), Expect = 2.3e-192
Identity = 353/546 (64.65%), Postives = 430/546 (78.75%), Query Frame = 0

Query: 20  NPKIPIPLA------RPSFP-FTYQSLSCPAIRLRSSFSTSSIDVPSRVSRRFVV---TG 79
           NPK+P P +       PSFP F   ++S P   L+ +    +    +  ++  VV   TG
Sbjct: 18  NPKLPFPSSLTLRSWNPSFPSFRSSAVSGPKSSLKLNRFLRNC---ASTNQELVVDGETG 77

Query: 80  DG--REIESEIA---VEVQENEQLLGSSNREELGSQGLWRQMKEIVTFTGPAIGLWICGP 139
           +G   E++ + A   +   E E  +     ++L +Q +W QMKEIV FTGPA GLW+CGP
Sbjct: 78  NGSISELQGDAANGSISPVEVEAEVEEVKVDDLATQSIWGQMKEIVMFTGPAAGLWLCGP 137

Query: 140 LMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATALAKQDKDEVQH 199
           LMSLIDTAVIGQGS++ELAALGPATV+CDY  Y FMFLS+ATSN+VAT+LA+QDKDEVQH
Sbjct: 138 LMSLIDTAVIGQGSSLELAALGPATVICDYLCYTFMFLSVATSNLVATSLARQDKDEVQH 197

Query: 200 HISVLLFVGLISGFLMLLATKLLGSVALTAFAGTKNADVIPAANTYVQIRGLAWPAILTG 259
            IS+LLF+GL  G  M++ T+L GS ALTAF G KNAD++PAAN YVQIRGLAWPA+L G
Sbjct: 198 QISILLFIGLACGVTMMVLTRLFGSWALTAFTGVKNADIVPAANKYVQIRGLAWPAVLIG 257

Query: 260 WVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATMASQVIAAYMM 319
           WVAQSASLGMKDSWGPLKALAVAS +NG+GDVVLC FLGYGIAGAAWATM SQV+AAYMM
Sbjct: 258 WVAQSASLGMKDSWGPLKALAVASAINGVGDVVLCTFLGYGIAGAAWATMVSQVVAAYMM 317

Query: 320 IETLNKKGYRGYSLSVPSPGEFLSILGLAAPVFITMMSKVVFYSLLIYYATSMGTHTMAA 379
           ++ LNKKGY  +S  VPSP E L+I GLAAPVFITMMSKV+FY+LL+Y+ATSMGT+ +AA
Sbjct: 318 MDALNKKGYSAFSFCVPSPSELLTIFGLAAPVFITMMSKVLFYTLLVYFATSMGTNIIAA 377

Query: 380 HQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVNPSLDKARMLLKSLLIIGAIFGLVLGT 439
           HQVM+Q + M TVWGEPLSQTAQSFMP L+ G+N +L KAR+LLKSL+IIGA  G+V+GT
Sbjct: 378 HQVMLQIYTMSTVWGEPLSQTAQSFMPELLFGINRNLPKARVLLKSLVIIGATLGIVVGT 437

Query: 440 IGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVITPPTHSLEGTLLAGRDLKFISLSM 499
           IGT+VPWLFP +FT ++ +  EMHKV+IPYFLAL ITP THSLEGTLLAGRDL++ISLSM
Sbjct: 438 IGTAVPWLFPGIFTRDKVVTSEMHKVIIPYFLALSITPSTHSLEGTLLAGRDLRYISLSM 497

Query: 500 TGCLCLGAIVLLAICSRGYGLAGCWYALVGFQWARFIIALRRVLSPNGVLYSSDLSHYKL 551
           TGCL +  ++L+ + + G+GL GCWYALVGFQWARF ++L R+LS +GVLYS D S Y  
Sbjct: 498 TGCLAVAGLLLMLLSNGGFGLRGCWYALVGFQWARFSLSLFRLLSRDGVLYSEDTSRY-A 557

BLAST of MS020574 vs. ExPASy Swiss-Prot
Match: Q945F0 (Protein DETOXIFICATION 47, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DTX47 PE=2 SV=1)

HSP 1 Score: 608.2 bits (1567), Expect = 9.1e-173
Identity = 314/507 (61.93%), Postives = 386/507 (76.13%), Query Frame = 0

Query: 43  AIRLRSSFSTSSIDVPSRVSRRFVVTGDGREIESEIAVEVQENEQLLGSSNREELGSQGL 102
           AI    SF  + + +  R+     VT +   I+ EI  E +E E+      R +L  Q +
Sbjct: 42  AITATPSFHKNPVVIRRRIKLE-RVTRNCVRIDREIDEEEEEEEK-----ERGDLVKQSI 101

Query: 103 WRQMKEIVTFTGPAIGLWICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFL 162
           W QMKEIV FTGPA+G+WICGPLMSLIDT VIGQGS++ELAALGP TVLCD+ SYVFMFL
Sbjct: 102 WEQMKEIVKFTGPAMGMWICGPLMSLIDTVVIGQGSSIELAALGPGTVLCDHMSYVFMFL 161

Query: 163 SIATSNMVATALAKQDKDEVQHHISVLLFVGLISGFLMLLATKLLGSVALTAFAGTKNAD 222
           S+ATSNMVAT+LAKQDK E QH ISVLLF+GL+ G +MLL T+L G  A+TAF   KN +
Sbjct: 162 SVATSNMVATSLAKQDKKEAQHQISVLLFIGLVCGLMMLLLTRLFGPWAVTAFTRGKNIE 221

Query: 223 VIPAANTYVQIRGLAWPAILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFL 282
           ++PAAN Y+QIRGLAWP IL G VAQSASLGMK+SWGPLKALA A+I+NG+GD +LC+FL
Sbjct: 222 IVPAANKYIQIRGLAWPFILVGLVAQSASLGMKNSWGPLKALAAATIINGLGDTILCLFL 281

Query: 283 GYGIAGAAWATMASQVIAAYMMIETLNKKGYRGYSLSVPSPGEFLSILGLAAPVFITMMS 342
           G GIAGAAWAT ASQ+++AYMM+++LNK+GY  YS ++PSP E   I  LAAPVFI++ S
Sbjct: 282 GQGIAGAAWATTASQIVSAYMMMDSLNKEGYNAYSFAIPSPQELWKISALAAPVFISIFS 341

Query: 343 KVVFYSLLIYYATSMGTHTMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVNPSLD 402
           K+ FYS +IY ATSMGTH +AAHQVM QT+ MC VWGEPLSQTAQSFMP ++ G N +L 
Sbjct: 342 KIAFYSFIIYCATSMGTHVLAAHQVMAQTYRMCNVWGEPLSQTAQSFMPEMLYGANRNLP 401

Query: 403 KARMLLKSLLIIGAIFGLVLGTIGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVITP 462
           KAR LLKSL+IIGA  GLVLG IGT+VP LFP ++T ++ II EMH++LIP+F+AL   P
Sbjct: 402 KARTLLKSLMIIGATLGLVLGVIGTAVPGLFPGVYTHDKVIISEMHRLLIPFFMALSALP 461

Query: 463 PTHSLEGTLLAGRDLKFISLSMTGCLCLGAIVLLAICSRGYGLAGCWYALVGFQWARFII 522
            T SLEGTLLAGRDLKF+S  M+    +G + L+ +   GYGL GCW+ LVGFQW RF +
Sbjct: 462 MTVSLEGTLLAGRDLKFVSSVMSSSFIIGCLTLMFVTRSGYGLLGCWFVLVGFQWGRFGL 521

Query: 523 ALRRVLSPNGVLYSSDLSHYKLEKQKA 550
            LRR+LSP G+L S   S Y +EK K+
Sbjct: 522 YLRRLLSPGGILNSDGPSPYTVEKIKS 542

BLAST of MS020574 vs. ExPASy Swiss-Prot
Match: Q84K71 (Protein DETOXIFICATION 44, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DTX44 PE=2 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.8e-19
Identity = 117/495 (23.64%), Postives = 218/495 (44.04%), Query Frame = 0

Query: 59  SRVSRRFVVTGDGREIESEIAVEVQENEQL---LGSSNR---EELGSQGLWRQMKEIVTF 118
           S + R  V     R+    ++   Q  E+    L S N+   +     G+ +   EI++ 
Sbjct: 24  SSIRRTIVCKSSPRDESPAVSTSSQRPEKQQNPLTSQNKPDHDHKPDPGIGKIGMEIMSI 83

Query: 119 TGPAIGLWICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVF--MFLSIATSNMV 178
             PA       P+ SL+DTA +G   + ELAA+G +  + +  S +F    L++ TS + 
Sbjct: 84  ALPAALALAADPITSLVDTAFVGHIGSAELAAVGVSVSVFNLVSKLFNVPLLNVTTSFVA 143

Query: 179 --ATALAKQDKDEVQ------HHISVLLFVGLISGFLMLLATKLLGSVALTAFAGTKNAD 238
                 AK D D ++        +S  L +    G    +A  L     +   A   ++ 
Sbjct: 144 EEQAIAAKDDNDSIETSKKVLPSVSTSLVLAAGVGIAEAIALSLGSDFLMDVMAIPFDSP 203

Query: 239 VIPAANTYVQIRGLAWPAILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFL 298
           +   A  ++++R    P I+    AQ A  G KD+  PL A+   +++N + D +L   L
Sbjct: 204 MRIPAEQFLRLRAYGAPPIVVALAAQGAFRGFKDTTTPLYAVVAGNVLNAVLDPILIFVL 263

Query: 299 GYGIAGAAWATMASQVIAAYMMIETLNKK-GYRGYSLSVPSPGEFLSILGLAAPVFITMM 358
           G+GI+GAA AT+ S+ + A++++  LN+        + V    ++L   GL     + ++
Sbjct: 264 GFGISGAAAATVISEYLIAFILLWKLNENVVLLSPQIKVGRANQYLKSGGLLIGRTVALL 323

Query: 359 SKVVFYSLLIYYATSMGTHTMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVNPSL 418
              V ++L    A   G   MA HQ++++ +   ++  + L+  AQS +    +      
Sbjct: 324 ---VPFTLATSLAAQNGPTQMAGHQIVLEIWLAVSLLTDALAIAAQSLLATTYS--QGEY 383

Query: 419 DKARMLLKSLLIIGAIFGLVLGTIGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVIT 478
            +AR +L  +L +G   G  L  +       F +LFT + +++    K+ +   L +  +
Sbjct: 384 KQAREVLFGVLQVGLATGTGLAAVLFITFEPFSSLFTTDSEVL----KIALSGTLFVAGS 443

Query: 479 PPTHS----LEGTLLAGRDLKFISLSMTGCLCLGAIVLLAICSRGYGLAGCWYALVGFQW 533
            P ++    L+G      D  F + SM     + ++ +L + +  +GLAG W  L  F  
Sbjct: 444 QPVNALAFVLDGLYYGVSDFGFAAYSMVIVGFISSLFML-VAAPTFGLAGIWTGLFLFMA 503

BLAST of MS020574 vs. ExPASy Swiss-Prot
Match: Q9SFB0 (Protein DETOXIFICATION 43 OS=Arabidopsis thaliana OX=3702 GN=DTX43 PE=1 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 2.0e-15
Identity = 117/498 (23.49%), Postives = 200/498 (40.16%), Query Frame = 0

Query: 107 KEIVTFTGPAIGLWICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMF--LSI 166
           +EI+    PA       P+ SLIDTA +G+  AV+LAA+G +  + +  S + +F  +S+
Sbjct: 35  REILGIAFPAALALAADPIASLIDTAFVGRLGAVQLAAVGVSIAIFNQASRITIFPLVSL 94

Query: 167 ATSNMVATALAKQDKDEVQ-----HHISVL------------------------------ 226
            TS +      ++ K+E       H  ++L                              
Sbjct: 95  TTSFVAEEDTMEKMKEEANKANLVHAETILVQDSLEKGISSPTSNDTNQPQQPPAPDTKS 154

Query: 227 ------------------------LFVGLISGFLMLLATKLLGSVALTAFAGTKNADVIP 286
                                   L +GL+    ++ ++KLL    L       N+ ++ 
Sbjct: 155 NSGNKSNKKEKRTIRTASTAMILGLILGLVQAIFLIFSSKLL----LGVMGVKPNSPMLS 214

Query: 287 AANTYVQIRGLAWPAILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYG 346
            A+ Y+ IR L  PA+L     Q    G KD+  PL A  VA ++N + D +    L  G
Sbjct: 215 PAHKYLSIRALGAPALLLSLAMQGIFRGFKDTKTPLFATVVADVINIVLDPIFIFVLRLG 274

Query: 347 IAGAAWATMASQVIAAYMMIETLNKKGYRGYSLSVPSPGE-----FL--SILGLAAPVFI 406
           I GAA A + SQ     ++   L KK     +L  P+ G+     FL   +L LA  + +
Sbjct: 275 IIGAAIAHVISQYFMTLILFVFLAKK----VNLIPPNFGDLQFGRFLKNGLLLLARTIAV 334

Query: 407 TMMSKVVFYSLLIYYATSMGTHTMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVN 466
           T        +L    A  +GT  MAA Q+ +Q +   ++  + L+   Q+ +    +   
Sbjct: 335 TFCQ-----TLAAAMAARLGTTPMAAFQICLQVWLTSSLLNDGLAVAGQAIL--ACSFAE 394

Query: 467 PSLDKARMLLKSLLIIGAIFGLVLGTIGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLAL 526
              +K   +   +L +G + GL L        +    +F+ +  +I  M  + IP+  A 
Sbjct: 395 KDYNKVTAVASRVLQMGFVLGLGLSVFVGLGLYFGAGVFSKDPAVIHLM-AIGIPFIAA- 454

Query: 527 VITPPTHS----LEGTLLAGRDLKFISLSMTGCLCLGAIVLLAICSRGYGLAGCWYALVG 533
             T P +S    L+G      D  + + SM G   + +I  +   ++  G  G W AL  
Sbjct: 455 --TQPINSLAFVLDGVNFGASDFAYTAYSMVGVAAI-SIAAVIYMAKTNGFIGIWIALTI 512

BLAST of MS020574 vs. ExPASy Swiss-Prot
Match: Q9SYD6 (Protein DETOXIFICATION 42 OS=Arabidopsis thaliana OX=3702 GN=DTX42 PE=2 SV=2)

HSP 1 Score: 70.1 bits (170), Expect = 8.9e-11
Identity = 105/483 (21.74%), Postives = 184/483 (38.10%), Query Frame = 0

Query: 108 EIVTFTGPAIGLWICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMF--LSIA 167
           EI     PA       P+ SL+DTA IGQ   VELAA+G +  L +  S + +F  +SI 
Sbjct: 35  EIARIALPAALALTADPIASLVDTAFIGQIGPVELAAVGVSIALFNQVSRIAIFPLVSIT 94

Query: 168 TSNMVATALAKQDKDEVQHH---------------------------------------- 227
           TS +         +D V+ H                                        
Sbjct: 95  TSFVAEEDACSSQQDTVRDHKECIEIGINNPTEETIELIPEKHKDSLSDEFKTSSSIFSI 154

Query: 228 ---------ISVLLFVGLISGFLMLLATKLLGSVA--LTAFAGTK-NADVIPAANTYVQI 287
                    I       +I G L L     L S A  L +F G K ++ ++  +  Y+ +
Sbjct: 155 SKPPAKKRNIPSASSALIIGGVLGLFQAVFLISAAKPLLSFMGVKHDSPMMRPSQRYLSL 214

Query: 288 RGLAWPAILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWAT 347
           R L  PA+L    AQ    G KD+  PL A  +  + N I D +       G+ GAA A 
Sbjct: 215 RSLGAPAVLLSLAAQGVFRGFKDTTTPLFATVIGDVTNIILDPIFIFVFRLGVTGAATAH 274

Query: 348 MASQVIAAYMMIETLNKKGYRGYSLSVPSPGEFLSILGLAAPVFITMMSKVVFYSLLIYY 407
           + SQ +   +++  L   G            +F   +     + + +++     +L    
Sbjct: 275 VISQYLMCGILLWKL--MGQVDIFNMSTKHLQFCRFMKNGFLLLMRVIAVTFCVTLSASL 334

Query: 408 ATSMGTHTMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVNPSLDKARMLLKSLLI 467
           A   G+ +MAA QV +Q +   ++  +  +   Q+ +            +A      +L 
Sbjct: 335 AAREGSTSMAAFQVCLQVWLATSLLADGYAVAGQAILASAF--AKKDYKRAAATASRVLQ 394

Query: 468 IGAIFGLVLGTIGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVITPPTHSL----EG 527
           +G + G VL  I  +       +FT ++K++  +  + +P+      T P ++L    +G
Sbjct: 395 LGLVLGFVLAVILGAGLHFGARVFTKDDKVL-HLISIGLPFVAG---TQPINALAFVFDG 454

Query: 528 TLLAGRDLKFISLSMTGCLCLGAIVLLAICSRGYGLAGCWYALVGFQWARFIIALRRVLS 533
                 D  + + S+   + + +I+ L   S  +G  G W+ L  +   R  +   R+ +
Sbjct: 455 VNFGASDFGYAAASLV-MVAIVSILCLLFLSSTHGFIGLWFGLTIYMSLRAAVGFWRIGT 508

BLAST of MS020574 vs. ExPASy TrEMBL
Match: A0A6J1CXJ2 (Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111015498 PE=3 SV=1)

HSP 1 Score: 1060.1 bits (2740), Expect = 3.2e-306
Identity = 546/550 (99.27%), Postives = 548/550 (99.64%), Query Frame = 0

Query: 1   MPFKILHRPSSITAQIQNHNPKIPIPLARPSFPFTYQSLSCPAIRLRSSFSTSSIDVPSR 60
           MPFKILHRPSSITAQIQN NPKIPIPLARPSFPFTYQSLSCPAIRLRSSFSTSSIDVPSR
Sbjct: 1   MPFKILHRPSSITAQIQNQNPKIPIPLARPSFPFTYQSLSCPAIRLRSSFSTSSIDVPSR 60

Query: 61  VSRRFVVTGDGREIESEIAVEVQENEQLLGSSNREELGSQGLWRQMKEIVTFTGPAIGLW 120
           VSRRFVVTGDGREIESEIAVEVQENEQLLGSSNREELGSQGLWRQMKEIVTFTGPAIGLW
Sbjct: 61  VSRRFVVTGDGREIESEIAVEVQENEQLLGSSNREELGSQGLWRQMKEIVTFTGPAIGLW 120

Query: 121 ICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATALAKQDKD 180
           ICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATALAKQDKD
Sbjct: 121 ICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATALAKQDKD 180

Query: 181 EVQHHISVLLFVGLISGFLMLLATKLLGSVALTAFAGTKNADVIPAANTYVQIRGLAWPA 240
           EVQHHISVLLFVGLISGFLMLLATKLLGSVALTAFAGTKNAD+IPAANTYVQIRGLAWPA
Sbjct: 181 EVQHHISVLLFVGLISGFLMLLATKLLGSVALTAFAGTKNADIIPAANTYVQIRGLAWPA 240

Query: 241 ILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATMASQVIA 300
           ILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATMASQVIA
Sbjct: 241 ILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATMASQVIA 300

Query: 301 AYMMIETLNKKGYRGYSLSVPSPGEFLSILGLAAPVFITMMSKVVFYSLLIYYATSMGTH 360
           AYMMIETLNKKGY GYSLSVPSPGEFLSILGLAAPVFITMMSKVVFYSLLIYYATSMGTH
Sbjct: 301 AYMMIETLNKKGYHGYSLSVPSPGEFLSILGLAAPVFITMMSKVVFYSLLIYYATSMGTH 360

Query: 361 TMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVNPSLDKARMLLKSLLIIGAIFGL 420
           TMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLI+GVNPSLDKARMLLKSLLIIGAIFGL
Sbjct: 361 TMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLIHGVNPSLDKARMLLKSLLIIGAIFGL 420

Query: 421 VLGTIGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVITPPTHSLEGTLLAGRDLKFI 480
           VLGTIGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVITPPTHSLEGTLLAGRDLKFI
Sbjct: 421 VLGTIGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVITPPTHSLEGTLLAGRDLKFI 480

Query: 481 SLSMTGCLCLGAIVLLAICSRGYGLAGCWYALVGFQWARFIIALRRVLSPNGVLYSSDLS 540
           SLSMTGCLCLGAIVLLAICSRGYGLAGCWYALVGFQWARFIIALRRVLSPNGVLYSSDLS
Sbjct: 481 SLSMTGCLCLGAIVLLAICSRGYGLAGCWYALVGFQWARFIIALRRVLSPNGVLYSSDLS 540

Query: 541 HYKLEKQKAA 551
           HYKLEKQKAA
Sbjct: 541 HYKLEKQKAA 550

BLAST of MS020574 vs. ExPASy TrEMBL
Match: A0A1S3CDR7 (Protein DETOXIFICATION OS=Cucumis melo OX=3656 GN=LOC103499716 PE=3 SV=1)

HSP 1 Score: 884.0 bits (2283), Expect = 3.2e-253
Identity = 472/556 (84.89%), Postives = 496/556 (89.21%), Query Frame = 0

Query: 1   MPFKILHRPSSITAQIQNHNPKIPIPLARPSFPFTYQSLSCPAIRLRSSFSTSSIDVP-- 60
           MPFKILH  SS +   + H P I  P +  S PFT+ SLS P I    +F +SSI  P  
Sbjct: 1   MPFKILHSSSSSSLVPRFHCPSILKPFSPSSLPFTHPSLSSPTI----TFPSSSISSPLP 60

Query: 61  ----SRVSRRFVVTGDGREIESEIAVEVQENEQLLGSSNREELGSQGLWRQMKEIVTFTG 120
               SR  RRF +     EIE EI +EVQ +EQ+L     EELG+QGL  QMKEIVTFTG
Sbjct: 61  PHFSSRNRRRFPIL--RVEIEREIGIEVQRDEQVL-EIEGEELGNQGLLNQMKEIVTFTG 120

Query: 121 PAIGLWICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATAL 180
           PAIGLWICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATAL
Sbjct: 121 PAIGLWICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATAL 180

Query: 181 AKQDKDEVQHHISVLLFVGLISGFLMLLATKLLGSVALTAFAGTKNADVIPAANTYVQIR 240
           AKQDK+EVQHHISVLLFVGL++GFLMLL+TKLLGSVALTAF GTKNAD+IPAANTY+QIR
Sbjct: 181 AKQDKNEVQHHISVLLFVGLVAGFLMLLSTKLLGSVALTAFVGTKNADIIPAANTYIQIR 240

Query: 241 GLAWPAILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATM 300
           GLAWPAILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATM
Sbjct: 241 GLAWPAILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATM 300

Query: 301 ASQVIAAYMMIETLNKKGYRGYSLSVPSPGEFLSILGLAAPVFITMMSKVVFYSLLIYYA 360
           ASQVIAAYMMIETLNKKGY GYSLSVPS GEFLSILGLAAPVF+TMMSKVVFYSLLIYYA
Sbjct: 301 ASQVIAAYMMIETLNKKGYNGYSLSVPSSGEFLSILGLAAPVFLTMMSKVVFYSLLIYYA 360

Query: 361 TSMGTHTMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVNPSLDKARMLLKSLLII 420
           TSMGTHTMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVN SLDKA MLLKSLLII
Sbjct: 361 TSMGTHTMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVNRSLDKAWMLLKSLLII 420

Query: 421 GAIFGLVLGTIGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVITPPTHSLEGTLLAG 480
           GAIFGLVLGTIGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVITPPTHSLEGTLLAG
Sbjct: 421 GAIFGLVLGTIGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVITPPTHSLEGTLLAG 480

Query: 481 RDLKFISLSMTGCLCLGAIVLLAICSRGYGLAGCWYALVGFQWARFIIALRRVLSPNGVL 540
           RDLK+ISLSMTGCL LGA+VLL I SRGYGL GCWYALVGFQWARF+ ALRR+LSP+GVL
Sbjct: 481 RDLKYISLSMTGCLSLGALVLLIISSRGYGLTGCWYALVGFQWARFLSALRRILSPDGVL 540

Query: 541 YSSDLSHYKLEKQKAA 551
           YSSDLSH +LEKQKAA
Sbjct: 541 YSSDLSHNELEKQKAA 549

BLAST of MS020574 vs. ExPASy TrEMBL
Match: A0A6J1JG71 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111484215 PE=3 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 7.1e-253
Identity = 467/553 (84.45%), Postives = 490/553 (88.61%), Query Frame = 0

Query: 5   ILHRPSSITAQIQNHNPKIPIPLARPSFPFTYQSLSCPAIRLRSSFSTSSIDVPSRVSRR 64
           ILH P S+TA+I  HNP    PL+ PSF         P I  RSS S  +ID+ SR++RR
Sbjct: 5   ILHSPFSVTARI--HNPNNLRPLSPPSF---------PTIFFRSSSSPLAIDLSSRINRR 64

Query: 65  FVVTGDGR-------EIESEIAVEVQENEQLLGSSNREELGSQGLWRQMKEIVTFTGPAI 124
           F V            EIESEI +EVQENEQLLGS   EELGSQGL  QMKEIVTFTGPAI
Sbjct: 65  FAVPRRRHEWEVGCLEIESEIGIEVQENEQLLGSGGGEELGSQGLLTQMKEIVTFTGPAI 124

Query: 125 GLWICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATALAKQ 184
           GLWICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATALAKQ
Sbjct: 125 GLWICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATALAKQ 184

Query: 185 DKDEVQHHISVLLFVGLISGFLMLLATKLLGSVALTAFAGTKNADVIPAANTYVQIRGLA 244
           DK+EVQHHISVLLFVGLISGFLMLL TKLLGSVALTAF GTKNAD+IPAANTY+QIRGLA
Sbjct: 185 DKNEVQHHISVLLFVGLISGFLMLLVTKLLGSVALTAFVGTKNADIIPAANTYIQIRGLA 244

Query: 245 WPAILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATMASQ 304
           WPAILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATM SQ
Sbjct: 245 WPAILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATMVSQ 304

Query: 305 VIAAYMMIETLNKKGYRGYSLSVPSPGEFLSILGLAAPVFITMMSKVVFYSLLIYYATSM 364
           VIAAYMMIE LNKK Y G++LSVPS GEF SILGLAAPVF+ MMSKVVFYSLLIYYATSM
Sbjct: 305 VIAAYMMIEALNKKAYNGFALSVPSSGEFFSILGLAAPVFLPMMSKVVFYSLLIYYATSM 364

Query: 365 GTHTMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVNPSLDKARMLLKSLLIIGAI 424
           GTH MAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVN +LDKARMLLKSLLIIGAI
Sbjct: 365 GTHIMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVNRNLDKARMLLKSLLIIGAI 424

Query: 425 FGLVLGTIGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVITPPTHSLEGTLLAGRDL 484
           FGL+LG+IGT VPWLFP LFTPEE+IIQEMHKVLIPYFLALVITPPTHSLEGTLLAGRDL
Sbjct: 425 FGLILGSIGTLVPWLFPYLFTPEERIIQEMHKVLIPYFLALVITPPTHSLEGTLLAGRDL 484

Query: 485 KFISLSMTGCLCLGAIVLLAICSRGYGLAGCWYALVGFQWARFIIALRRVLSPNGVLYSS 544
           KFISLSMTGCL LGA+VLL I SRGYGL GCWYAL GFQWARF+IALRR+LSP+G+LYSS
Sbjct: 485 KFISLSMTGCLSLGALVLLVISSRGYGLTGCWYALTGFQWARFLIALRRILSPDGMLYSS 544

Query: 545 DLSHYKLEKQKAA 551
           DLSHYKLEK KAA
Sbjct: 545 DLSHYKLEKPKAA 546

BLAST of MS020574 vs. ExPASy TrEMBL
Match: A0A6J1FWF3 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111448773 PE=3 SV=1)

HSP 1 Score: 875.9 bits (2262), Expect = 8.6e-251
Identity = 468/554 (84.48%), Postives = 487/554 (87.91%), Query Frame = 0

Query: 5   ILHRPSSITAQIQNHNPKIPIPLARPSFPFTYQSLSCPAIRLRSSFSTSSIDVPSRVSRR 64
           ILH P S+TA+I  HNP     L+ PSF         P I  RSS S  +ID  SR++RR
Sbjct: 5   ILHSPFSVTARI--HNPNNLRQLSPPSF---------PTIFFRSSSSPLAIDFSSRINRR 64

Query: 65  FVVTGDGR-------EIESEIAVEVQENEQLLGSS-NREELGSQGLWRQMKEIVTFTGPA 124
             V            EIESEI +EVQENE L GS    EELGSQGL  QMKEIVTFTGPA
Sbjct: 65  LAVPRRRHEWEVGCLEIESEIGIEVQENELLSGSGVGGEELGSQGLLTQMKEIVTFTGPA 124

Query: 125 IGLWICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATALAK 184
           IGLWICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATALAK
Sbjct: 125 IGLWICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATALAK 184

Query: 185 QDKDEVQHHISVLLFVGLISGFLMLLATKLLGSVALTAFAGTKNADVIPAANTYVQIRGL 244
           QDK+EVQHHISVLLFVGLISGFLMLL TKLLGSVALTAF GTKNAD+IPAANTY+QIRGL
Sbjct: 185 QDKNEVQHHISVLLFVGLISGFLMLLVTKLLGSVALTAFVGTKNADIIPAANTYIQIRGL 244

Query: 245 AWPAILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATMAS 304
           AWPAILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATM S
Sbjct: 245 AWPAILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATMVS 304

Query: 305 QVIAAYMMIETLNKKGYRGYSLSVPSPGEFLSILGLAAPVFITMMSKVVFYSLLIYYATS 364
           QVIAAYMMIETLNKK Y GYSLSVPS GEF SILGLAAPVF+TMMSKVVFYSLLIYYATS
Sbjct: 305 QVIAAYMMIETLNKKAYNGYSLSVPSSGEFFSILGLAAPVFLTMMSKVVFYSLLIYYATS 364

Query: 365 MGTHTMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVNPSLDKARMLLKSLLIIGA 424
           MGTHTMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVN SLDKARMLLKSLLIIG 
Sbjct: 365 MGTHTMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVNRSLDKARMLLKSLLIIGT 424

Query: 425 IFGLVLGTIGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVITPPTHSLEGTLLAGRD 484
           IFGL+LG+IGT VPWLFP LFTPEE+IIQEMHKVLIPYFLALVITPPTHSLEGTLLAGRD
Sbjct: 425 IFGLILGSIGTLVPWLFPYLFTPEERIIQEMHKVLIPYFLALVITPPTHSLEGTLLAGRD 484

Query: 485 LKFISLSMTGCLCLGAIVLLAICSRGYGLAGCWYALVGFQWARFIIALRRVLSPNGVLYS 544
           LKFISLSMTGCL LGA+VLL I SRGYGL GCWYAL GFQWARF+IALRR+LSP+G+LYS
Sbjct: 485 LKFISLSMTGCLSLGALVLLVISSRGYGLTGCWYALTGFQWARFLIALRRILSPDGMLYS 544

Query: 545 SDLSHYKLEKQKAA 551
           SDLSHYKLEK KAA
Sbjct: 545 SDLSHYKLEKPKAA 547

BLAST of MS020574 vs. ExPASy TrEMBL
Match: A0A0A0LJ49 (Protein DETOXIFICATION OS=Cucumis sativus OX=3659 GN=Csa_2G250950 PE=3 SV=1)

HSP 1 Score: 865.1 bits (2234), Expect = 1.5e-247
Identity = 464/551 (84.21%), Postives = 489/551 (88.75%), Query Frame = 0

Query: 1   MPFKILHRPSSITAQIQNHNPKIPIPLARPSFPFTYQSLSCPAIRLRSS--FSTSSIDVP 60
           MPFKILH  SS     + H P I  P +  SFP T   LS P I   SS   S S +   
Sbjct: 1   MPFKILHSSSSSFVP-RFHCPNILKPFSPSSFPLTRPFLSSPTISFPSSSISSPSPLHFS 60

Query: 61  SRVSRRFVVTGDGREIESEIAVEVQENEQLLGSSNREELGSQGLWRQMKEIVTFTGPAIG 120
           SR  RRF V     EIE EI +EVQ++EQ+LG    EELG+QGL  Q+KEIVTFTGPAIG
Sbjct: 61  SRNRRRFPVL--RVEIEREIGIEVQKDEQVLGIEG-EELGNQGLLNQLKEIVTFTGPAIG 120

Query: 121 LWICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATALAKQD 180
           LWICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATALAKQD
Sbjct: 121 LWICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATALAKQD 180

Query: 181 KDEVQHHISVLLFVGLISGFLMLLATKLLGSVALTAFAGTKNADVIPAANTYVQIRGLAW 240
           K+EVQHHISVLLFVGL++GFLMLL+TKLLGSVALTAF G KNAD+IPAANTY+QIRGLAW
Sbjct: 181 KNEVQHHISVLLFVGLMAGFLMLLSTKLLGSVALTAFVGAKNADIIPAANTYIQIRGLAW 240

Query: 241 PAILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATMASQV 300
           PAILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATMASQ+
Sbjct: 241 PAILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATMASQI 300

Query: 301 IAAYMMIETLNKKGYRGYSLSVPSPGEFLSILGLAAPVFITMMSKVVFYSLLIYYATSMG 360
           IAAYMMIE LNKKGY GYSLSVPS GEFLSILGLAAPVF+TMMSKVVFYSLLIYYATSMG
Sbjct: 301 IAAYMMIEALNKKGYDGYSLSVPSSGEFLSILGLAAPVFLTMMSKVVFYSLLIYYATSMG 360

Query: 361 THTMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVNPSLDKARMLLKSLLIIGAIF 420
           THTMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVN SLDKA MLLKSL+IIGAIF
Sbjct: 361 THTMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVNRSLDKAWMLLKSLMIIGAIF 420

Query: 421 GLVLGTIGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVITPPTHSLEGTLLAGRDLK 480
           GLVLGTIGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVITPPTHSLEGTLLAGRDLK
Sbjct: 421 GLVLGTIGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVITPPTHSLEGTLLAGRDLK 480

Query: 481 FISLSMTGCLCLGAIVLLAICSRGYGLAGCWYALVGFQWARFIIALRRVLSPNGVLYSSD 540
           +ISLSMTGCL LGA+VLL I +RGYGL GCWYALVGFQWARF+ ALRR+LSPNG+L SSD
Sbjct: 481 YISLSMTGCLSLGALVLLIISTRGYGLTGCWYALVGFQWARFLSALRRILSPNGIL-SSD 540

Query: 541 LSHYKLEKQKA 550
           LSH +LEKQKA
Sbjct: 541 LSHNELEKQKA 546

BLAST of MS020574 vs. TAIR 10
Match: AT2G21340.1 (MATE efflux family protein )

HSP 1 Score: 673.3 bits (1736), Expect = 1.6e-193
Identity = 353/546 (64.65%), Postives = 430/546 (78.75%), Query Frame = 0

Query: 20  NPKIPIPLA------RPSFP-FTYQSLSCPAIRLRSSFSTSSIDVPSRVSRRFVV---TG 79
           NPK+P P +       PSFP F   ++S P   L+ +    +    +  ++  VV   TG
Sbjct: 18  NPKLPFPSSLTLRSWNPSFPSFRSSAVSGPKSSLKLNRFLRNC---ASTNQELVVDGETG 77

Query: 80  DG--REIESEIA---VEVQENEQLLGSSNREELGSQGLWRQMKEIVTFTGPAIGLWICGP 139
           +G   E++ + A   +   E E  +     ++L +Q +W QMKEIV FTGPA GLW+CGP
Sbjct: 78  NGSISELQGDAANGSISPVEVEAEVEEVKVDDLATQSIWGQMKEIVMFTGPAAGLWLCGP 137

Query: 140 LMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATALAKQDKDEVQH 199
           LMSLIDTAVIGQGS++ELAALGPATV+CDY  Y FMFLS+ATSN+VAT+LA+QDKDEVQH
Sbjct: 138 LMSLIDTAVIGQGSSLELAALGPATVICDYLCYTFMFLSVATSNLVATSLARQDKDEVQH 197

Query: 200 HISVLLFVGLISGFLMLLATKLLGSVALTAFAGTKNADVIPAANTYVQIRGLAWPAILTG 259
            IS+LLF+GL  G  M++ T+L GS ALTAF G KNAD++PAAN YVQIRGLAWPA+L G
Sbjct: 198 QISILLFIGLACGVTMMVLTRLFGSWALTAFTGVKNADIVPAANKYVQIRGLAWPAVLIG 257

Query: 260 WVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATMASQVIAAYMM 319
           WVAQSASLGMKDSWGPLKALAVAS +NG+GDVVLC FLGYGIAGAAWATM SQV+AAYMM
Sbjct: 258 WVAQSASLGMKDSWGPLKALAVASAINGVGDVVLCTFLGYGIAGAAWATMVSQVVAAYMM 317

Query: 320 IETLNKKGYRGYSLSVPSPGEFLSILGLAAPVFITMMSKVVFYSLLIYYATSMGTHTMAA 379
           ++ LNKKGY  +S  VPSP E L+I GLAAPVFITMMSKV+FY+LL+Y+ATSMGT+ +AA
Sbjct: 318 MDALNKKGYSAFSFCVPSPSELLTIFGLAAPVFITMMSKVLFYTLLVYFATSMGTNIIAA 377

Query: 380 HQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVNPSLDKARMLLKSLLIIGAIFGLVLGT 439
           HQVM+Q + M TVWGEPLSQTAQSFMP L+ G+N +L KAR+LLKSL+IIGA  G+V+GT
Sbjct: 378 HQVMLQIYTMSTVWGEPLSQTAQSFMPELLFGINRNLPKARVLLKSLVIIGATLGIVVGT 437

Query: 440 IGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVITPPTHSLEGTLLAGRDLKFISLSM 499
           IGT+VPWLFP +FT ++ +  EMHKV+IPYFLAL ITP THSLEGTLLAGRDL++ISLSM
Sbjct: 438 IGTAVPWLFPGIFTRDKVVTSEMHKVIIPYFLALSITPSTHSLEGTLLAGRDLRYISLSM 497

Query: 500 TGCLCLGAIVLLAICSRGYGLAGCWYALVGFQWARFIIALRRVLSPNGVLYSSDLSHYKL 551
           TGCL +  ++L+ + + G+GL GCWYALVGFQWARF ++L R+LS +GVLYS D S Y  
Sbjct: 498 TGCLAVAGLLLMLLSNGGFGLRGCWYALVGFQWARFSLSLFRLLSRDGVLYSEDTSRY-A 557

BLAST of MS020574 vs. TAIR 10
Match: AT2G21340.2 (MATE efflux family protein )

HSP 1 Score: 664.5 bits (1713), Expect = 7.6e-191
Identity = 351/546 (64.29%), Postives = 428/546 (78.39%), Query Frame = 0

Query: 20  NPKIPIPLA------RPSFP-FTYQSLSCPAIRLRSSFSTSSIDVPSRVSRRFVV---TG 79
           NPK+P P +       PSFP F   ++S P   L+ +    +    +  ++  VV   TG
Sbjct: 18  NPKLPFPSSLTLRSWNPSFPSFRSSAVSGPKSSLKLNRFLRNC---ASTNQELVVDGETG 77

Query: 80  DG--REIESEIA---VEVQENEQLLGSSNREELGSQGLWRQMKEIVTFTGPAIGLWICGP 139
           +G   E++ + A   +   E E  +     ++L +Q +W QMKEIV FTGPA GLW+CGP
Sbjct: 78  NGSISELQGDAANGSISPVEVEAEVEEVKVDDLATQSIWGQMKEIVMFTGPAAGLWLCGP 137

Query: 140 LMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFLSIATSNMVATALAKQDKDEVQH 199
           LMSLIDTAVIGQGS++ELAALGPATV+CDY  Y FMFLS+ATSN+VAT+LA+QDKDEVQH
Sbjct: 138 LMSLIDTAVIGQGSSLELAALGPATVICDYLCYTFMFLSVATSNLVATSLARQDKDEVQH 197

Query: 200 HISVLLFVGLISGFLMLLATKLLGSVALTAFAGTKNADVIPAANTYVQIRGLAWPAILTG 259
            IS+LLF+GL  G  M++ T+L GS ALT   G KNAD++PAAN YVQIRGLAWPA+L G
Sbjct: 198 QISILLFIGLACGVTMMVLTRLFGSWALT---GVKNADIVPAANKYVQIRGLAWPAVLIG 257

Query: 260 WVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYGIAGAAWATMASQVIAAYMM 319
           WVAQSASLGMKDSWGPLKALAVAS +NG+GDVVLC FLGYGIAGAAWATM SQV+AAYMM
Sbjct: 258 WVAQSASLGMKDSWGPLKALAVASAINGVGDVVLCTFLGYGIAGAAWATMVSQVVAAYMM 317

Query: 320 IETLNKKGYRGYSLSVPSPGEFLSILGLAAPVFITMMSKVVFYSLLIYYATSMGTHTMAA 379
           ++ LNKKGY  +S  VPSP E L+I GLAAPVFITMMSKV+FY+LL+Y+ATSMGT+ +AA
Sbjct: 318 MDALNKKGYSAFSFCVPSPSELLTIFGLAAPVFITMMSKVLFYTLLVYFATSMGTNIIAA 377

Query: 380 HQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVNPSLDKARMLLKSLLIIGAIFGLVLGT 439
           HQVM+Q + M TVWGEPLSQTAQSFMP L+ G+N +L KAR+LLKSL+IIGA  G+V+GT
Sbjct: 378 HQVMLQIYTMSTVWGEPLSQTAQSFMPELLFGINRNLPKARVLLKSLVIIGATLGIVVGT 437

Query: 440 IGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVITPPTHSLEGTLLAGRDLKFISLSM 499
           IGT+VPWLFP +FT ++ +  EMHKV+IPYFLAL ITP THSLEGTLLAGRDL++ISLSM
Sbjct: 438 IGTAVPWLFPGIFTRDKVVTSEMHKVIIPYFLALSITPSTHSLEGTLLAGRDLRYISLSM 497

Query: 500 TGCLCLGAIVLLAICSRGYGLAGCWYALVGFQWARFIIALRRVLSPNGVLYSSDLSHYKL 551
           TGCL +  ++L+ + + G+GL GCWYALVGFQWARF ++L R+LS +GVLYS D S Y  
Sbjct: 498 TGCLAVAGLLLMLLSNGGFGLRGCWYALVGFQWARFSLSLFRLLSRDGVLYSEDTSRY-A 556

BLAST of MS020574 vs. TAIR 10
Match: AT4G39030.1 (MATE efflux family protein )

HSP 1 Score: 608.2 bits (1567), Expect = 6.5e-174
Identity = 314/507 (61.93%), Postives = 386/507 (76.13%), Query Frame = 0

Query: 43  AIRLRSSFSTSSIDVPSRVSRRFVVTGDGREIESEIAVEVQENEQLLGSSNREELGSQGL 102
           AI    SF  + + +  R+     VT +   I+ EI  E +E E+      R +L  Q +
Sbjct: 42  AITATPSFHKNPVVIRRRIKLE-RVTRNCVRIDREIDEEEEEEEK-----ERGDLVKQSI 101

Query: 103 WRQMKEIVTFTGPAIGLWICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMFL 162
           W QMKEIV FTGPA+G+WICGPLMSLIDT VIGQGS++ELAALGP TVLCD+ SYVFMFL
Sbjct: 102 WEQMKEIVKFTGPAMGMWICGPLMSLIDTVVIGQGSSIELAALGPGTVLCDHMSYVFMFL 161

Query: 163 SIATSNMVATALAKQDKDEVQHHISVLLFVGLISGFLMLLATKLLGSVALTAFAGTKNAD 222
           S+ATSNMVAT+LAKQDK E QH ISVLLF+GL+ G +MLL T+L G  A+TAF   KN +
Sbjct: 162 SVATSNMVATSLAKQDKKEAQHQISVLLFIGLVCGLMMLLLTRLFGPWAVTAFTRGKNIE 221

Query: 223 VIPAANTYVQIRGLAWPAILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFL 282
           ++PAAN Y+QIRGLAWP IL G VAQSASLGMK+SWGPLKALA A+I+NG+GD +LC+FL
Sbjct: 222 IVPAANKYIQIRGLAWPFILVGLVAQSASLGMKNSWGPLKALAAATIINGLGDTILCLFL 281

Query: 283 GYGIAGAAWATMASQVIAAYMMIETLNKKGYRGYSLSVPSPGEFLSILGLAAPVFITMMS 342
           G GIAGAAWAT ASQ+++AYMM+++LNK+GY  YS ++PSP E   I  LAAPVFI++ S
Sbjct: 282 GQGIAGAAWATTASQIVSAYMMMDSLNKEGYNAYSFAIPSPQELWKISALAAPVFISIFS 341

Query: 343 KVVFYSLLIYYATSMGTHTMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVNPSLD 402
           K+ FYS +IY ATSMGTH +AAHQVM QT+ MC VWGEPLSQTAQSFMP ++ G N +L 
Sbjct: 342 KIAFYSFIIYCATSMGTHVLAAHQVMAQTYRMCNVWGEPLSQTAQSFMPEMLYGANRNLP 401

Query: 403 KARMLLKSLLIIGAIFGLVLGTIGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVITP 462
           KAR LLKSL+IIGA  GLVLG IGT+VP LFP ++T ++ II EMH++LIP+F+AL   P
Sbjct: 402 KARTLLKSLMIIGATLGLVLGVIGTAVPGLFPGVYTHDKVIISEMHRLLIPFFMALSALP 461

Query: 463 PTHSLEGTLLAGRDLKFISLSMTGCLCLGAIVLLAICSRGYGLAGCWYALVGFQWARFII 522
            T SLEGTLLAGRDLKF+S  M+    +G + L+ +   GYGL GCW+ LVGFQW RF +
Sbjct: 462 MTVSLEGTLLAGRDLKFVSSVMSSSFIIGCLTLMFVTRSGYGLLGCWFVLVGFQWGRFGL 521

Query: 523 ALRRVLSPNGVLYSSDLSHYKLEKQKA 550
            LRR+LSP G+L S   S Y +EK K+
Sbjct: 522 YLRRLLSPGGILNSDGPSPYTVEKIKS 542

BLAST of MS020574 vs. TAIR 10
Match: AT2G38330.1 (MATE efflux family protein )

HSP 1 Score: 99.0 bits (245), Expect = 1.3e-20
Identity = 117/495 (23.64%), Postives = 218/495 (44.04%), Query Frame = 0

Query: 59  SRVSRRFVVTGDGREIESEIAVEVQENEQL---LGSSNR---EELGSQGLWRQMKEIVTF 118
           S + R  V     R+    ++   Q  E+    L S N+   +     G+ +   EI++ 
Sbjct: 24  SSIRRTIVCKSSPRDESPAVSTSSQRPEKQQNPLTSQNKPDHDHKPDPGIGKIGMEIMSI 83

Query: 119 TGPAIGLWICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVF--MFLSIATSNMV 178
             PA       P+ SL+DTA +G   + ELAA+G +  + +  S +F    L++ TS + 
Sbjct: 84  ALPAALALAADPITSLVDTAFVGHIGSAELAAVGVSVSVFNLVSKLFNVPLLNVTTSFVA 143

Query: 179 --ATALAKQDKDEVQ------HHISVLLFVGLISGFLMLLATKLLGSVALTAFAGTKNAD 238
                 AK D D ++        +S  L +    G    +A  L     +   A   ++ 
Sbjct: 144 EEQAIAAKDDNDSIETSKKVLPSVSTSLVLAAGVGIAEAIALSLGSDFLMDVMAIPFDSP 203

Query: 239 VIPAANTYVQIRGLAWPAILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFL 298
           +   A  ++++R    P I+    AQ A  G KD+  PL A+   +++N + D +L   L
Sbjct: 204 MRIPAEQFLRLRAYGAPPIVVALAAQGAFRGFKDTTTPLYAVVAGNVLNAVLDPILIFVL 263

Query: 299 GYGIAGAAWATMASQVIAAYMMIETLNKK-GYRGYSLSVPSPGEFLSILGLAAPVFITMM 358
           G+GI+GAA AT+ S+ + A++++  LN+        + V    ++L   GL     + ++
Sbjct: 264 GFGISGAAAATVISEYLIAFILLWKLNENVVLLSPQIKVGRANQYLKSGGLLIGRTVALL 323

Query: 359 SKVVFYSLLIYYATSMGTHTMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVNPSL 418
              V ++L    A   G   MA HQ++++ +   ++  + L+  AQS +    +      
Sbjct: 324 ---VPFTLATSLAAQNGPTQMAGHQIVLEIWLAVSLLTDALAIAAQSLLATTYS--QGEY 383

Query: 419 DKARMLLKSLLIIGAIFGLVLGTIGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLALVIT 478
            +AR +L  +L +G   G  L  +       F +LFT + +++    K+ +   L +  +
Sbjct: 384 KQAREVLFGVLQVGLATGTGLAAVLFITFEPFSSLFTTDSEVL----KIALSGTLFVAGS 443

Query: 479 PPTHS----LEGTLLAGRDLKFISLSMTGCLCLGAIVLLAICSRGYGLAGCWYALVGFQW 533
            P ++    L+G      D  F + SM     + ++ +L + +  +GLAG W  L  F  
Sbjct: 444 QPVNALAFVLDGLYYGVSDFGFAAYSMVIVGFISSLFML-VAAPTFGLAGIWTGLFLFMA 503

BLAST of MS020574 vs. TAIR 10
Match: AT3G08040.1 (MATE efflux family protein )

HSP 1 Score: 85.5 bits (210), Expect = 1.5e-16
Identity = 117/498 (23.49%), Postives = 200/498 (40.16%), Query Frame = 0

Query: 107 KEIVTFTGPAIGLWICGPLMSLIDTAVIGQGSAVELAALGPATVLCDYTSYVFMF--LSI 166
           +EI+    PA       P+ SLIDTA +G+  AV+LAA+G +  + +  S + +F  +S+
Sbjct: 35  REILGIAFPAALALAADPIASLIDTAFVGRLGAVQLAAVGVSIAIFNQASRITIFPLVSL 94

Query: 167 ATSNMVATALAKQDKDEVQ-----HHISVL------------------------------ 226
            TS +      ++ K+E       H  ++L                              
Sbjct: 95  TTSFVAEEDTMEKMKEEANKANLVHAETILVQDSLEKGISSPTSNDTNQPQQPPAPDTKS 154

Query: 227 ------------------------LFVGLISGFLMLLATKLLGSVALTAFAGTKNADVIP 286
                                   L +GL+    ++ ++KLL    L       N+ ++ 
Sbjct: 155 NSGNKSNKKEKRTIRTASTAMILGLILGLVQAIFLIFSSKLL----LGVMGVKPNSPMLS 214

Query: 287 AANTYVQIRGLAWPAILTGWVAQSASLGMKDSWGPLKALAVASIVNGIGDVVLCMFLGYG 346
            A+ Y+ IR L  PA+L     Q    G KD+  PL A  VA ++N + D +    L  G
Sbjct: 215 PAHKYLSIRALGAPALLLSLAMQGIFRGFKDTKTPLFATVVADVINIVLDPIFIFVLRLG 274

Query: 347 IAGAAWATMASQVIAAYMMIETLNKKGYRGYSLSVPSPGE-----FL--SILGLAAPVFI 406
           I GAA A + SQ     ++   L KK     +L  P+ G+     FL   +L LA  + +
Sbjct: 275 IIGAAIAHVISQYFMTLILFVFLAKK----VNLIPPNFGDLQFGRFLKNGLLLLARTIAV 334

Query: 407 TMMSKVVFYSLLIYYATSMGTHTMAAHQVMIQTFCMCTVWGEPLSQTAQSFMPGLINGVN 466
           T        +L    A  +GT  MAA Q+ +Q +   ++  + L+   Q+ +    +   
Sbjct: 335 TFCQ-----TLAAAMAARLGTTPMAAFQICLQVWLTSSLLNDGLAVAGQAIL--ACSFAE 394

Query: 467 PSLDKARMLLKSLLIIGAIFGLVLGTIGTSVPWLFPNLFTPEEKIIQEMHKVLIPYFLAL 526
              +K   +   +L +G + GL L        +    +F+ +  +I  M  + IP+  A 
Sbjct: 395 KDYNKVTAVASRVLQMGFVLGLGLSVFVGLGLYFGAGVFSKDPAVIHLM-AIGIPFIAA- 454

Query: 527 VITPPTHS----LEGTLLAGRDLKFISLSMTGCLCLGAIVLLAICSRGYGLAGCWYALVG 533
             T P +S    L+G      D  + + SM G   + +I  +   ++  G  G W AL  
Sbjct: 455 --TQPINSLAFVLDGVNFGASDFAYTAYSMVGVAAI-SIAAVIYMAKTNGFIGIWIALTI 512