MS020549 (gene) Bitter gourd (TR) v1

Overview


Name	MS020549
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Bifunctional dihydrofolate reductase-thymidylate synthase
Location	scaffold375: 1089540 .. 1095505 (-)
RNA-Seq Expression	MS020549
Synteny	MS020549

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGGTGATCCCTTGACAAGTTCTAATGGCAACGCTAATGTCATTGCACCACCTGATCCGCGGAGGACTTTCCAAGTTGTCGTGGCTGCTACTGAAAATATGGGTATTGGTAAGGATGGGAAGCTACCATGGAGGCTGCCTTCTGATCTCAAATATTTTAAAAACATAACCACAACAACATCAGATCATGGGAAAAGGAATGCAGTTCTAATGGGTCGAAAAACATGGGAGAGTATTCCTGCTGAGCATCGACCACTACCTGGTCGTCTCAATGTTGTTCTAACTCGTTCGGGGAGTTTTGACATTGCAACTGTTGAAAATGTTATAATATGTGGTAGTATAACGTCTGCTTTGGAATTATTAGCAGCTTCTCCTTACTGTTTGTCAATAGAGAAAGTGTTTATCATAGGCGGTGGTGAGATATTAAGGTGTGCCCCTGTTTTCCAGTCCTCTCTTAGTGTATGCTATTTATTATTTCTGTGCACTGATTTTGTGTTTAATGCAGGGAAGCTCTGAATGCCCCTGAATGTGATGCTATCCATGTCACAGAAATTCAGACATGTGTCGAATGTGATACCTTCATACCAGCTATTGATAAGTCTATGTTTCAGCCATGGTACTCATCCTTTCCTGTGGTGGAAAATAACCTTCGATATTCTTTCACTAGTTATGTTCGTGTGAGGAGCTCAATTGAACCACCCGTTGATCAGAACAATGGTCTGGTCTCTTATAGTAGGCCCGATTCTTCTAATTTTGAGGCTAAGAAGTTCTCTTTCCTGCCTAAGGAGATTTTTGAAAGGCACGAGGAGTACTTGTATCTAAATCTTGTTAAGGAAATCATCTCAGATGGTACTTCTAAGGATGATAGAACAGGAACTGGTACTTTGTCAAGATTCGGTGTCCAGGTAATGTCATCTTATCATTTGGAATGTCTTTTTTTTTTTTCCTACCAATTGCTTTATTTCAAATTCTAATGCGCTTTCAAGTTCATGTCTTGTTCCTGGTATATGTACAGATGCGATACAACTTGCGCAAAACTTTTCCACTGCTTACTACTAAGGCATGTGCCAATTTCCTATTTTGATTTCTTTCTAGCTGGATCGTGAAAGCGAAACTCCCTCTTTTCTTGTTTTGTTTCCCTTTTGCAATTTCTAGGAATCTCGTGCATCATTTATGGAAATGGTCCAGTTGTTGATATATATCCTTACTGTTTGATTCTTCAGAAAGTGTTTTGGCGAGGGGTTGTTGAAGAACTTCTTTGGTTCATCAGTGGGTCAACCAACGCAAAGGTTAAATGCTATCATAACTTTAAGCTTTACTTTACTAGTAGGTGGTTGTTATTCTATTTTTTTCCCTCTCTGTAGTATTTTCCATTAGGAATTAGATTGAGCCTTGTGACCTAGACTAGATGATCTGCTCCATTTATGAAGCGGTCTGCTCAGTGGTTCTTCCCATCATTTGTTTATTTATTTTTTAATAAGAAACCAAGCTTTCATAGAGAGAAATGAACATACGGAATACACCTAACTGATAATTACAAAAACCTTAGAAACTGACCCCACAGAAAGTATTAAATCTCAAGACCCCAAACAAACTCCCTTGATCTCTTCACCCATAAAAATTCTATTGTTTCTCTCAAGTCAAATGCCCACCATCTGTTTATGTAATTAGCTTAAGATTCCTTCTGCATTCATTTTATTGTTTCTCTCAAGCCAAATGGCTGCTATCTGTTTATGTAATTAGCTTAAGATTCCTTCTGCGTTCATATGATAAATATTATGACCGTACGTATAAATAATCAACACTTAACCGTGGTGAAAAGTGATTATAAAAAATCAATCTAATTGACATTGGTAAAATAAGTATTATAATTACAAAAGAGTCCACCTAGTGAAGCACCAAGGAGAAGCTTTTGACTGATCTCTCAAATTTGGTTAATACTTATACTTTTACCATTGAAGACTTGTGGGTTTTTCTTTTTTAGCCAAGTTTCTCATATAGCACGTTTCCAGTTTTGACTGTGCATTTTCAAATAATCATCACTTGTTTTGAAGGGGTGGCCTAAAAATAGTTATTATATTGCTCGAGTCGTGGAGCAAAGATGACACAAATGAAACTTGGAGTGTTGGAAGGGAGAGCCCAAGTATTTTGATTTCTGAGTAGTTTGATAGTGTAGAATATATGGGGAAGCGCCATTCTTGGAAATTTCTTTTGTTACTTTCCAGTTGTATTTAAACTTTTCAACAGAGACACCACAAGAGAAATCTTACTTTTGGGGGTATTGTTGACTCCAGATAGTGTTATAAACATGTTGATCATGGACAGAGTTGTGTGAGGGAGGACATATAGGAGTCAAAGTTGTGAGTAACTGAGTTGCAAAGCTTCCAAATTAAATTATACTCTCTAGAGTCAGGAAGACAGAAATTTAGAAGGTGAACTAGTTGTGAGTAACTGAGTTGCAAAGCTTCCAAATTAAATTGTACTCTCTAGAGTCAGGAAGACAGAGATTTAGAAGGTGAACTAGTTGCAGAAAGTCCTAATTTCAGGTCACGAAGAGATCCAGTAAAGGTGATAAGCCAATAGTTGCTTCATATTGACCGTCCTCCACACCAGCACATTTTTTGGTTCGAGAGTTGGAAGAGTCTGGCATTTTTTTAAATATATGTATGTGAAACCGATCTTTCATTAAAAAAAAAAATAAAAGAATATACTATGGCATACAAAAAACAGCCCAGCAAAAGGAGCCCAATAAATTGCATAAAGGGACCCCAATCCAAAAGAATACCCACTAAAAAATATGAGCTTCAAGGTTTATCAGCTACCCAAATTAAATTTCTGAAGCAGTATGGTGGAAGGGATAATGTAGGTCTCCTTCTTGCAGTTTGTCAATAACACTATCTTATTCTGATTGAGCAAGAATGTTGCTTTTGTTATCTCTAAAATACTTTTCAAGTCATTGGCTCTAATGTTGATCTTAGAGTCAATAGGAAAAAATATTTTGTAGTTGGTATTTTGTCTCAAAATTTTTTTTTGGTAGTTGGTATTAATGTGATTAGGCTATAAGCTTGTTTAGGGGTGGGCTCAGTTATCCTTCTTCCTTGACTTATATCTTTCTTTGGGCCATAGTATGACATTTCTTCTTTGGGATCCAATTGTGGAAAAGGTTCACCTTTTATGTGAAGGGGTGAATCAGAGGGGTGCGGTTCTCAAGTCAGAATTTCAAGGCATGGGAGAAGGAAGGGGTAGGAATCGGGAATTTAGTAATTAAGTATTTTGCTTGCTAGTCTTTGTGGTTGTGGTTTTTCCCTTTATAGCTTTAGACACTGTTGGTTGGTAGGGTACAAACATCACTCTAATGGGTAGCTGGATACTATTATGATGACTAAAGGCACTTTGGAACTTATGGAAAGTGATTTCAGTCTCCCTAGTTTCCTCAATTTCATTAAATTCTCCCTAGAAGATGGATTTCGTGTTTGTTTCTAGGAGGGTCATTGGATTGGTTATGGTCTTCTTTGTTTGCTTTGCATTTTTACCCTTCATTTTCTCAATCACTCTTTGTGTAGTCTATTCCTAATAGACGTGCACATTCCTTTGTTATCTCTATTTGGGGGTTTTTTTTTCTCTCATAACTTCCTTGTTAAGGGAACTTTATTTGGTAGGGAAGCTGTAGATGTGTCATTGACTCCTTTTATCTCTCTCGAATGGTCTTCCTCTCCAAGCACTTGGATCCTCAAGTCCTTTGATGTGCTTTATTGTAAAGCCTGTCTCCTTTTGCTTGCTTACTCCATCTTCTTCATAACCCTTTTTTTAGTAAATATTTTCATTGAAGATGTCAAGAGTACATCATAAGCCCGAGGAGATTTTAGAAAACCATGAATTGACATTAATAAGAAAAACGAGTTTTTACACAAGGTTTTGACAAGAGTAATTCAGGTTTAGAGCAATATAGGAAGGTTACTGGAGGGAGAGGGACGTGGTTGGGTTTAGAGCAGTATAGGAAGGTTTTGCAATCTGTCTCCGGCTGCATGGTTACTGGAGGGACAGGCTTGATTTAGCAGTATCTTGGGTATCTCTTTCTTGGAGTGCACTATCTATATTTTGTTGCAATCCTGTCCCCATGGTATTAATACATATTGGATTGCTTTTTCTGTAAACTCCTAATGGGAGTATAATTGCATGATGGCAGTAACTTTGAGAAGAGAGATCGGAGAAGTATCTAGAATGGGAGAGAGATACTGCTAAGGATACCTTTTTTTTTTCCTGGTAAACTCCTAATGGGGATGCCCAAATCCTACGGTACATTTGGCTCCAAAGGAACATGCATATTTCTTAAGGGAGTACATTTGGCTCCAAAGGAACAGGAATATTTTTTAAGGGAGTAGAGGGAGGATGTCTGAAATCTTGCTAATGTTAATTCCTCCCTTTGGGGCTTTGTATCTAAGGGTGCTTTTCCTCTCTGTGATTACAAATGATTGGAGGCCTTTATTGCAATTATCACTTATTGTAATTATTTTTTGGTGGCTATTGCAATCTTGTTTTGGCCTATGATTTTATATGGCCTTTTTTGTAACCTTCAAGTGGTCTTTTGGCCAAAAAAAGAAGAATTTTTGCATCCTAGGAATAGCATGTGCAAATAAATATACACATCATTATTAAGAATGAGGATTATGAAGGAATTGATATAAATGAATGCCTTTTCAAGTTGACTTGTTTTATATCCTAAAGTTCGGCAATATCCACAGGTTCTGCAGGAGAAAGGCATTCATATATGGGATGGCAATGCATCCAGAGATTACCTCGATAGGCAATTACTTATACATTTTCATAATTTGAATTTGTTCAATATAAAGTTTAAGCTATAATTCTGTTTTTTCTCCTCTTTTCTTTTCTTCTTCCTCTTCCTTTTCCCCTTTTCTTTGTAATCTTAGTATTGGTTTGAGCGAAAGGGAGGAGGGTGACCTGGGACCTGTATACGGGTTCCAGTGGCGACACTTTGGTGCTAGGTTCGTGGATTATTCCAAGGATCTCTTTGAAGTTTGTTTCTATTTTATTGCAGGATTTGAGCTTTAATGCTTTCCACATTCTTCTACAGGTATACTGACATGCATGCTGACTACACTGGTCAAGGATTCGATCAGTTGCTAGATGTTATTAACAAGATAAAAACTAATCCCAATGACCGGAGGATAATTCTTTCCGCATGGAACCCATCTGATCTCAAACTGATGGCCCTTCCGCCTTGCCACATGTTCGCTCAGGTTGGATTCAAGTTCATGGTTCTTTGTTCTCCATTCATTTCCTTACATTATTCATTTATCTTTTTGCAGTTTTATGTAGCGAATGGGGAGTTGTCGTGTCAAATGTATCAGCGCTCTGCTGACATGGGCCTTGGCGTTCCATTTAATATTGCATCATATGCTCTTCTGACATGCATGATTGCTCATGTTTGTGGTATGCTACTCTTTTTTGCTTTAGTACAATGGAGATGGCTGAATGTTTAAATTACTTAGTTGCAATCTGTTTAATGATAAAGTTATAAAATGAAATTTATTTCTCATCAACTTGGCATTTAAAAATCTCTGAACAAAATAAAGCGGTTTTCCTCCTCTACGGTTATACTTTTAGCTACATTTAGAAACTATTCTTCCTTCCCACGTGCAGATCTCGCTCAAGGCGACTTCATACATGTGATTGGGGACGCTCATGTTTACCATACTCATGTGAGGCCATTGCAGGAGCAGCTCCAGAAACTCCCCAAACCTTTCCCTGTAAGTAGAAAAACTATAATTATGCAATTCTTCCGAGCGTTTATTACCAGGCTGTTTATAATATACACCAACCTTACGTTTCTGTAGATTTTGAAGATTAATCCTGAGAAGAAGGATATCGATTCTTTCATAGCAGCTGATTTCGAACTAACCGGTTACGACCCTCATGAAAAGATAGCAATGAAAATGGCAGTA

mRNA sequence

ATGGCTGGTGATCCCTTGACAAGTTCTAATGGCAACGCTAATGTCATTGCACCACCTGATCCGCGGAGGACTTTCCAAGTTGTCGTGGCTGCTACTGAAAATATGGGTATTGGTAAGGATGGGAAGCTACCATGGAGGCTGCCTTCTGATCTCAAATATTTTAAAAACATAACCACAACAACATCAGATCATGGGAAAAGGAATGCAGTTCTAATGGGTCGAAAAACATGGGAGAGTATTCCTGCTGAGCATCGACCACTACCTGGTCGTCTCAATGTTGTTCTAACTCGTTCGGGGAGTTTTGACATTGCAACTGTTGAAAATGTTATAATATGTGGTAGTATAACGTCTGCTTTGGAATTATTAGCAGCTTCTCCTTACTGTTTGTCAATAGAGAAAGTGTTTATCATAGGCGGTGGTGAGATATTAAGGGAAGCTCTGAATGCCCCTGAATGTGATGCTATCCATGTCACAGAAATTCAGACATGTGTCGAATGTGATACCTTCATACCAGCTATTGATAAGTCTATGTTTCAGCCATGGTACTCATCCTTTCCTGTGGTGGAAAATAACCTTCGATATTCTTTCACTAGTTATGTTCGTGTGAGGAGCTCAATTGAACCACCCGTTGATCAGAACAATGGTCTGGTCTCTTATAGTAGGCCCGATTCTTCTAATTTTGAGGCTAAGAAGTTCTCTTTCCTGCCTAAGGAGATTTTTGAAAGGCACGAGGAGTACTTGTATCTAAATCTTGTTAAGGAAATCATCTCAGATGGTACTTCTAAGGATGATAGAACAGGAACTGGTACTTTGTCAAGATTCGGTGTCCAGATGCGATACAACTTGCGCAAAACTTTTCCACTGCTTACTACTAAGGCATTGTTTTGGCGAGGGGTTGTTGAAGAACTTCTTTGGTTCATCAGTGGGTCAACCAACGCAAAGGTTCTGCAGGAGAAAGGCATTCATATATGGGATGGCAATGCATCCAGAGATTACCTCGATAGGCAATTACTTATACATTTTCATAATTTGAATTTTATTGGTTTGAGCGAAAGGGAGGAGGGTGACCTGGGACCTGTATACGGGTTCCAGTGGCGACACTTTGGTGCTAGGTATACTGACATGCATGCTGACTACACTGGTCAAGGATTCGATCAGTTGCTAGATGTTATTAACAAGATAAAAACTAATCCCAATGACCGGAGGATAATTCTTTCCGCATGGAACCCATCTGATCTCAAACTGATGGCCCTTCCGCCTTGCCACATGTTCGCTCAGGTTGGATTCAAGTTCATGGTTCTTTGTTCTCCATTCATTTCCTTACATTATTCATTTATCTTTTTGCAGTTTTATGTAGCGAATGGGGAGTTGTCGTGTCAAATGTATCAGCGCTCTGCTGACATGGGCCTTGGCGTTCCATTTAATATTGCATCATATGCTCTTCTGACATGCATGATTGCTCATGTTTGTGATCTCGCTCAAGGCGACTTCATACATGTGATTGGGGACGCTCATGTTTACCATACTCATGTGAGGCCATTGCAGGAGCAGCTCCAGAAACTCCCCAAACCTTTCCCTATTTTGAAGATTAATCCTGAGAAGAAGGATATCGATTCTTTCATAGCAGCTGATTTCGAACTAACCGGTTACGACCCTCATGAAAAGATAGCAATGAAAATGGCAGTA

Coding sequence (CDS)

ATGGCTGGTGATCCCTTGACAAGTTCTAATGGCAACGCTAATGTCATTGCACCACCTGATCCGCGGAGGACTTTCCAAGTTGTCGTGGCTGCTACTGAAAATATGGGTATTGGTAAGGATGGGAAGCTACCATGGAGGCTGCCTTCTGATCTCAAATATTTTAAAAACATAACCACAACAACATCAGATCATGGGAAAAGGAATGCAGTTCTAATGGGTCGAAAAACATGGGAGAGTATTCCTGCTGAGCATCGACCACTACCTGGTCGTCTCAATGTTGTTCTAACTCGTTCGGGGAGTTTTGACATTGCAACTGTTGAAAATGTTATAATATGTGGTAGTATAACGTCTGCTTTGGAATTATTAGCAGCTTCTCCTTACTGTTTGTCAATAGAGAAAGTGTTTATCATAGGCGGTGGTGAGATATTAAGGGAAGCTCTGAATGCCCCTGAATGTGATGCTATCCATGTCACAGAAATTCAGACATGTGTCGAATGTGATACCTTCATACCAGCTATTGATAAGTCTATGTTTCAGCCATGGTACTCATCCTTTCCTGTGGTGGAAAATAACCTTCGATATTCTTTCACTAGTTATGTTCGTGTGAGGAGCTCAATTGAACCACCCGTTGATCAGAACAATGGTCTGGTCTCTTATAGTAGGCCCGATTCTTCTAATTTTGAGGCTAAGAAGTTCTCTTTCCTGCCTAAGGAGATTTTTGAAAGGCACGAGGAGTACTTGTATCTAAATCTTGTTAAGGAAATCATCTCAGATGGTACTTCTAAGGATGATAGAACAGGAACTGGTACTTTGTCAAGATTCGGTGTCCAGATGCGATACAACTTGCGCAAAACTTTTCCACTGCTTACTACTAAGGCATTGTTTTGGCGAGGGGTTGTTGAAGAACTTCTTTGGTTCATCAGTGGGTCAACCAACGCAAAGGTTCTGCAGGAGAAAGGCATTCATATATGGGATGGCAATGCATCCAGAGATTACCTCGATAGGCAATTACTTATACATTTTCATAATTTGAATTTTATTGGTTTGAGCGAAAGGGAGGAGGGTGACCTGGGACCTGTATACGGGTTCCAGTGGCGACACTTTGGTGCTAGGTATACTGACATGCATGCTGACTACACTGGTCAAGGATTCGATCAGTTGCTAGATGTTATTAACAAGATAAAAACTAATCCCAATGACCGGAGGATAATTCTTTCCGCATGGAACCCATCTGATCTCAAACTGATGGCCCTTCCGCCTTGCCACATGTTCGCTCAGGTTGGATTCAAGTTCATGGTTCTTTGTTCTCCATTCATTTCCTTACATTATTCATTTATCTTTTTGCAGTTTTATGTAGCGAATGGGGAGTTGTCGTGTCAAATGTATCAGCGCTCTGCTGACATGGGCCTTGGCGTTCCATTTAATATTGCATCATATGCTCTTCTGACATGCATGATTGCTCATGTTTGTGATCTCGCTCAAGGCGACTTCATACATGTGATTGGGGACGCTCATGTTTACCATACTCATGTGAGGCCATTGCAGGAGCAGCTCCAGAAACTCCCCAAACCTTTCCCTATTTTGAAGATTAATCCTGAGAAGAAGGATATCGATTCTTTCATAGCAGCTGATTTCGAACTAACCGGTTACGACCCTCATGAAAAGATAGCAATGAAAATGGCAGTA

Protein sequence

MAGDPLTSSNGNANVIAPPDPRRTFQVVVAATENMGIGKDGKLPWRLPSDLKYFKNITTTTSDHGKRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALELLAASPYCLSIEKVFIIGGGEILREALNAPECDAIHVTEIQTCVECDTFIPAIDKSMFQPWYSSFPVVENNLRYSFTSYVRVRSSIEPPVDQNNGLVSYSRPDSSNFEAKKFSFLPKEIFERHEEYLYLNLVKEIISDGTSKDDRTGTGTLSRFGVQMRYNLRKTFPLLTTKALFWRGVVEELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDRQLLIHFHNLNFIGLSEREEGDLGPVYGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKTNPNDRRIILSAWNPSDLKLMALPPCHMFAQVGFKFMVLCSPFISLHYSFIFLQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAQGDFIHVIGDAHVYHTHVRPLQEQLQKLPKPFPILKINPEKKDIDSFIAADFELTGYDPHEKIAMKMAV

Homology

[Close]

BLAST of MS020549 vs. NCBI nr
Match: XP_022146106.1 (bifunctional dihydrofolate reductase-thymidylate synthase 1-like [Momordica charantia])

HSP 1 Score: 1059.7 bits (2739), Expect = 8.9e-306
Identity = 523/562 (93.06%), Postives = 526/562 (93.59%), Query Frame = 0


Query: 1   MAGDPLTSSNGNANVIAPPDPRRTFQVVVAATENMGIGKDGKLPWRLPSDLKYFKNITTT 60
           MAGDPLTSSNGNANVIAPPDPRRTFQVVVAATENMGIGKDGKLPWRLPSDLKYFKNITTT
Sbjct: 10  MAGDPLTSSNGNANVIAPPDPRRTFQVVVAATENMGIGKDGKLPWRLPSDLKYFKNITTT 69

Query: 61  TSDHGKRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE 120
           TSDHGKRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE
Sbjct: 70  TSDHGKRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE 129

Query: 121 LLAASPYCLSIEKVFIIGGGEILREALNAPECDAIHVTEIQTCVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVFIIGGGEILREALNAPECDAIHVT+IQTCVECDTFIPAIDKSMFQP
Sbjct: 130 LLAASPYCLSIEKVFIIGGGEILREALNAPECDAIHVTDIQTCVECDTFIPAIDKSMFQP 189

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSIEPPVDQNNGLVSYSRPDSSNFEAKKFSFLPKEIF 240
           WYSSFPVVENNLRYSFTSYVRVRSSIEPP+DQNNGLVSYSRPDSSNFEAKKFSFLPKEIF
Sbjct: 190 WYSSFPVVENNLRYSFTSYVRVRSSIEPPIDQNNGLVSYSRPDSSNFEAKKFSFLPKEIF 249

Query: 241 ERHEEYLYLNLVKEIISDGTSKDDRTGTGTLSRFGVQMRYNLRKTFPLLTTKALFWRGVV 300
           ERHEEYLYLNLVKEIISDGTSKDDRTGTGTLSRFGVQMRYNLRKTFPLLTTK +FWRGVV
Sbjct: 250 ERHEEYLYLNLVKEIISDGTSKDDRTGTGTLSRFGVQMRYNLRKTFPLLTTKKVFWRGVV 309

Query: 301 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDRQLLIHFHNLNFIGLSEREEGDLGPV 360
           EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLD            IGLSEREEGDLGPV
Sbjct: 310 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDS-----------IGLSEREEGDLGPV 369

Query: 361 YGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKTNPNDRRIILSAWNPSDLKLMALPP 420
           YGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKTNPNDRRIILSAWNPSDLKLMALPP
Sbjct: 370 YGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKTNPNDRRIILSAWNPSDLKLMALPP 429

Query: 421 CHMFAQVGFKFMVLCSPFISLHYSFIFLQFYVANGELSCQMYQRSADMGLGVPFNIASYA 480
           CHMFA                       QFYVANGELSCQMYQRSADMGLGVPFNIASYA
Sbjct: 430 CHMFA-----------------------QFYVANGELSCQMYQRSADMGLGVPFNIASYA 489

Query: 481 LLTCMIAHVCDLAQGDFIHVIGDAHVYHTHVRPLQEQLQKLPKPFPILKINPEKKDIDSF 540
           LLTCMIAHVCDLAQGDFIHVIGDAHVYHTHVRPLQEQLQKLPKPFPILKINPEKKDIDSF
Sbjct: 490 LLTCMIAHVCDLAQGDFIHVIGDAHVYHTHVRPLQEQLQKLPKPFPILKINPEKKDIDSF 537

Query: 541 IAADFELTGYDPHEKIAMKMAV 563
           IAADFELTGYDPHEKIAMKMAV
Sbjct: 550 IAADFELTGYDPHEKIAMKMAV 537

[Close]

BLAST of MS020549 vs. NCBI nr
Match: XP_022944443.1 (bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita moschata] >XP_022944444.1 bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita moschata])

HSP 1 Score: 1008.1 bits (2605), Expect = 3.1e-290
Identity = 492/562 (87.54%), Postives = 513/562 (91.28%), Query Frame = 0


Query: 1   MAGDPLTSSNGNANVIAPPDPRRTFQVVVAATENMGIGKDGKLPWRLPSDLKYFKNITTT 60
           MA DPLTSSNGNANV APP+PRRT+QVVVAATENMGIGKDGKLPWRLPSDLK+FKNIT T
Sbjct: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITAT 60

Query: 61  TSDHGKRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE 120
           TSDHGKRNA+LMGRKTWESIPAE+RPLPGRLNVVLTRSGSFDIATVENVIICGS+TSALE
Sbjct: 61  TSDHGKRNAILMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120

Query: 121 LLAASPYCLSIEKVFIIGGGEILREALNAPECDAIHVTEIQTCVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVF+IGGGEILREALNAPECDA+HVTEI+T VECDTFIPAIDKSMFQP
Sbjct: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSIEPPVDQNNGLVSYSRPDSSNFEAKKFSFLPKEIF 240
           WYSSFPVVENNLRYSFTSYVRVRSS EPP+DQNNGLVS+++PDS NFEAK FSFLPKEIF
Sbjct: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEIF 240

Query: 241 ERHEEYLYLNLVKEIISDGTSKDDRTGTGTLSRFGVQMRYNLRKTFPLLTTKALFWRGVV 300
           ERHEEYLYL LV+EIISDGTSKDDRTGTGTLS+FG QMRYNLR+TFPLLTTK +FWRGVV
Sbjct: 241 ERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRRTFPLLTTKKVFWRGVV 300

Query: 301 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDRQLLIHFHNLNFIGLSEREEGDLGPV 360
           EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLD            IGL EREEGDLGPV
Sbjct: 301 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDS-----------IGLREREEGDLGPV 360

Query: 361 YGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKTNPNDRRIILSAWNPSDLKLMALPP 420
           YGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIK NP+DRRI+LSAWNPSDLKLMALPP
Sbjct: 361 YGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPP 420

Query: 421 CHMFAQVGFKFMVLCSPFISLHYSFIFLQFYVANGELSCQMYQRSADMGLGVPFNIASYA 480
           CHMFA                       QFYVANGELSCQMYQRSADMGLGVPFNIASYA
Sbjct: 421 CHMFA-----------------------QFYVANGELSCQMYQRSADMGLGVPFNIASYA 480

Query: 481 LLTCMIAHVCDLAQGDFIHVIGDAHVYHTHVRPLQEQLQKLPKPFPILKINPEKKDIDSF 540
           LLTCMIAHVCDLA GDFIHVIGDAHVY THVRPLQEQLQKLPKPFP+LKINPEKKDIDSF
Sbjct: 481 LLTCMIAHVCDLAYGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPVLKINPEKKDIDSF 528

Query: 541 IAADFELTGYDPHEKIAMKMAV 563
           +AADFELTGYDPHEKIAMKMAV
Sbjct: 541 VAADFELTGYDPHEKIAMKMAV 528

[Close]

BLAST of MS020549 vs. NCBI nr
Match: XP_023513067.1 (bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita pepo subsp. pepo] >XP_023513068.1 bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1004.6 bits (2596), Expect = 3.4e-289
Identity = 494/563 (87.74%), Postives = 513/563 (91.12%), Query Frame = 0


Query: 1   MAGDPLTSSNGNANVIAPPDPRRTFQVVVAATENMGIGKDGKLPWRLPSDLKYFKNITTT 60
           MA DPLTSSNGNANV APP+PRRT+QVVVAATENMGIGKDGKLPWRLPSDLK+FKNITTT
Sbjct: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTT 60

Query: 61  TSDHGKRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE 120
           TSDHGKRNAVLMGRKTWESIPAE+RPLPGRLNVVLTRSGSFDIATVENVIICGS+TSALE
Sbjct: 61  TSDHGKRNAVLMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120

Query: 121 LLAASPYCLSIEKVFIIGGGEILREALNAPECDAIHVTEIQTCVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVF+IGGGEILREALNAPECDA+HVTEI+T VECDTFIPAIDKSMFQP
Sbjct: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSIE-PPVDQNNGLVSYSRPDSSNFEAKKFSFLPKEI 240
           WYSSFPVVENNLRYSFTSYVRVRSS E PP+DQNNGLVS+++PDS NFEAK FSFLPKEI
Sbjct: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEI 240

Query: 241 FERHEEYLYLNLVKEIISDGTSKDDRTGTGTLSRFGVQMRYNLRKTFPLLTTKALFWRGV 300
           FERHEEYLYL LV+EIISDGTSKDDRTGTGTLS+FG QMRYNLRKTFPLLTTK +FWRGV
Sbjct: 241 FERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTKKVFWRGV 300

Query: 301 VEELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDRQLLIHFHNLNFIGLSEREEGDLGP 360
           VEELLWFISGSTNAKVLQEKGIHIWDGNASRDYLD            IGL EREEGDLGP
Sbjct: 301 VEELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDS-----------IGLREREEGDLGP 360

Query: 361 VYGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKTNPNDRRIILSAWNPSDLKLMALP 420
           VYGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIK NP+DRRI+LSAWNPSDLKLMALP
Sbjct: 361 VYGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALP 420

Query: 421 PCHMFAQVGFKFMVLCSPFISLHYSFIFLQFYVANGELSCQMYQRSADMGLGVPFNIASY 480
           PCHMFA                       QFYVANGELSCQMYQRSADMGLGVPFNIASY
Sbjct: 421 PCHMFA-----------------------QFYVANGELSCQMYQRSADMGLGVPFNIASY 480

Query: 481 ALLTCMIAHVCDLAQGDFIHVIGDAHVYHTHVRPLQEQLQKLPKPFPILKINPEKKDIDS 540
           ALLTCMIAHVCDLA GDFIHVIGDAHVY THVRPLQEQLQKLPKPFP+LKINPEKKDIDS
Sbjct: 481 ALLTCMIAHVCDLAYGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPVLKINPEKKDIDS 529

Query: 541 FIAADFELTGYDPHEKIAMKMAV 563
            +AADFELTGYDPHEKIAMKMAV
Sbjct: 541 LVAADFELTGYDPHEKIAMKMAV 529

[Close]

BLAST of MS020549 vs. NCBI nr
Match: XP_038901596.1 (bifunctional dihydrofolate reductase-thymidylate synthase 1-like [Benincasa hispida] >XP_038901597.1 bifunctional dihydrofolate reductase-thymidylate synthase 1-like [Benincasa hispida])

HSP 1 Score: 1004.2 bits (2595), Expect = 4.4e-289
Identity = 490/562 (87.19%), Postives = 512/562 (91.10%), Query Frame = 0


Query: 1   MAGDPLTSSNGNANVIAPPDPRRTFQVVVAATENMGIGKDGKLPWRLPSDLKYFKNITTT 60
           MAGDPLTSSNG ANV APPDPRRT+QVVVAA+ENMGIGKDGKLPWRLPSDLK+FKNITTT
Sbjct: 1   MAGDPLTSSNGIANVNAPPDPRRTYQVVVAASENMGIGKDGKLPWRLPSDLKFFKNITTT 60

Query: 61  TSDHGKRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE 120
           TSDHGKRNA+LMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE
Sbjct: 61  TSDHGKRNAILMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE 120

Query: 121 LLAASPYCLSIEKVFIIGGGEILREALNAPECDAIHVTEIQTCVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVF++GGGEILREALNAPECDAIH+TE++T +ECDTFIPAIDKSMFQP
Sbjct: 121 LLAASPYCLSIEKVFVVGGGEILREALNAPECDAIHITEVETNIECDTFIPAIDKSMFQP 180

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSIEPPVDQNNGLVSYSRPDSSNFEAKKFSFLPKEIF 240
           WYSSFPVVENNLRYSFTSYVRVRSS EPP+DQNNGLVS+++PD SNFEAKKFSFLPKEIF
Sbjct: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDFSNFEAKKFSFLPKEIF 240

Query: 241 ERHEEYLYLNLVKEIISDGTSKDDRTGTGTLSRFGVQMRYNLRKTFPLLTTKALFWRGVV 300
           E+HEEYLYLNLV+EIIS+G  KDDRTGTGTLSRFG QMRYNLRKTFPLLTTK +FWRGVV
Sbjct: 241 EKHEEYLYLNLVQEIISNGIPKDDRTGTGTLSRFGCQMRYNLRKTFPLLTTKKVFWRGVV 300

Query: 301 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDRQLLIHFHNLNFIGLSEREEGDLGPV 360
           EELLWFISGSTNA VLQEKGIHIWDGNASRDYLD            IGL EREEGDLGPV
Sbjct: 301 EELLWFISGSTNANVLQEKGIHIWDGNASRDYLDS-----------IGLREREEGDLGPV 360

Query: 361 YGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKTNPNDRRIILSAWNPSDLKLMALPP 420
           YGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIK NP+DRRIILSAWNPSDLKLMALPP
Sbjct: 361 YGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPP 420

Query: 421 CHMFAQVGFKFMVLCSPFISLHYSFIFLQFYVANGELSCQMYQRSADMGLGVPFNIASYA 480
           CHMFA                       QFYVANGELSCQMYQRSADMGLGVPFNIASYA
Sbjct: 421 CHMFA-----------------------QFYVANGELSCQMYQRSADMGLGVPFNIASYA 480

Query: 481 LLTCMIAHVCDLAQGDFIHVIGDAHVYHTHVRPLQEQLQKLPKPFPILKINPEKKDIDSF 540
           LLTCMIAHVC LA+GDFIHVIGDAHVY THVRPLQEQLQKLPKPFPILKINPEKKDIDSF
Sbjct: 481 LLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPILKINPEKKDIDSF 528

Query: 541 IAADFELTGYDPHEKIAMKMAV 563
           ++ DFELTGYDPHEKIAMKMAV
Sbjct: 541 VSDDFELTGYDPHEKIAMKMAV 528

[Close]

BLAST of MS020549 vs. NCBI nr
Match: XP_022986497.1 (bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita maxima])

HSP 1 Score: 1000.7 bits (2586), Expect = 4.9e-288
Identity = 491/562 (87.37%), Postives = 511/562 (90.93%), Query Frame = 0


Query: 1   MAGDPLTSSNGNANVIAPPDPRRTFQVVVAATENMGIGKDGKLPWRLPSDLKYFKNITTT 60
           MA DPLTSSNGNANV APP+PRRT+QVVVAATENMGIGKDGKLPWRLPSDLK+FKNITTT
Sbjct: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTT 60

Query: 61  TSDHGKRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE 120
           TSDHGKRNAVLMGRKTWESIPAE+RPLPGRLNVVLTRSGSFDIATVENVIICGS+TSALE
Sbjct: 61  TSDHGKRNAVLMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120

Query: 121 LLAASPYCLSIEKVFIIGGGEILREALNAPECDAIHVTEIQTCVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVF+IGGGEILREALNAPECDA+HVTEI+T VECDTFIPAIDKSMFQP
Sbjct: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSIEPPVDQNNGLVSYSRPDSSNFEAKKFSFLPKEIF 240
           WYSSFP+VENNLRYSFTSYVRVRSS EPP+DQNNGLVS+++PDS N   K FSFLPKEIF
Sbjct: 181 WYSSFPMVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFN---KNFSFLPKEIF 240

Query: 241 ERHEEYLYLNLVKEIISDGTSKDDRTGTGTLSRFGVQMRYNLRKTFPLLTTKALFWRGVV 300
           ERHEEYLYL LV+EIISDGTSKDDRTGTGTLS+FG QMRYNLRKTFPLLTTK +FWRGVV
Sbjct: 241 ERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTKKVFWRGVV 300

Query: 301 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDRQLLIHFHNLNFIGLSEREEGDLGPV 360
           EELLWFISGSTNAK+LQEKGIHIWDGNASRDYLD            IGL EREEGDLGPV
Sbjct: 301 EELLWFISGSTNAKILQEKGIHIWDGNASRDYLDS-----------IGLREREEGDLGPV 360

Query: 361 YGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKTNPNDRRIILSAWNPSDLKLMALPP 420
           YGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIK NPNDRRI+LSAWNPSDLKLMALPP
Sbjct: 361 YGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKNNPNDRRIVLSAWNPSDLKLMALPP 420

Query: 421 CHMFAQVGFKFMVLCSPFISLHYSFIFLQFYVANGELSCQMYQRSADMGLGVPFNIASYA 480
           CHMFA                       QFYVANGELSCQMYQRSADMGLGVPFNIASYA
Sbjct: 421 CHMFA-----------------------QFYVANGELSCQMYQRSADMGLGVPFNIASYA 480

Query: 481 LLTCMIAHVCDLAQGDFIHVIGDAHVYHTHVRPLQEQLQKLPKPFPILKINPEKKDIDSF 540
           LLTCMIAHVCDLA GDFIHVIGDAHVY THVRPLQEQLQKLPKPFP+LKINPEKKDIDSF
Sbjct: 481 LLTCMIAHVCDLAYGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPVLKINPEKKDIDSF 525

Query: 541 IAADFELTGYDPHEKIAMKMAV 563
           +AADFELTGYDPHEKIAMKMAV
Sbjct: 541 VAADFELTGYDPHEKIAMKMAV 525

[Close]

BLAST of MS020549 vs. ExPASy Swiss-Prot
Match: P51820 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Glycine max OX=3847 PE=1 SV=1)

HSP 1 Score: 834.3 bits (2154), Expect = 8.0e-241
Identity = 408/564 (72.34%), Postives = 462/564 (81.91%), Query Frame = 0


Query: 1   MAGDPLTSSNGNANVIAPPDP--RRTFQVVVAATENMGIGKDGKLPWRLPSDLKYFKNIT 60
           M  D    SNG++N    P P  +RT+QVVVAAT++ GIGKDGKLPWRLP+DLK+FK IT
Sbjct: 1   MPSDSSVISNGHSNGSVNPLPNLQRTYQVVVAATQDWGIGKDGKLPWRLPTDLKFFKEIT 60

Query: 61  TTTSDHGKRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSA 120
             TS+ GK+NA++MGRKTWESIP E+RPL GRLNVVLTRSGSFDIAT ENV+ICGS++SA
Sbjct: 61  MKTSEPGKKNAIVMGRKTWESIPLEYRPLSGRLNVVLTRSGSFDIATAENVVICGSMSSA 120

Query: 121 LELLAASPYCLSIEKVFIIGGGEILREALNAPECDAIHVTEIQTCVECDTFIPAIDKSMF 180
           LELLAASPY LSIEKVF+IGGG+I REALN P C+AIH+TEIQ+ +ECDTF+P +D ++F
Sbjct: 121 LELLAASPYSLSIEKVFVIGGGQIFREALNVPGCEAIHLTEIQSSIECDTFMPPVDFTIF 180

Query: 181 QPWYSSFPVVENNLRYSFTSYVRVRSSIEPPVDQNNGLVSYSRPDSSNFEAKKFSFLPKE 240
           +PWYSSFP VENN+RYSFT+YVRVRSS      QN   +  +  +S  FE K FSFLPK 
Sbjct: 181 RPWYSSFPKVENNIRYSFTTYVRVRSSAAESAGQNIDPLLDNNSESMKFEVKDFSFLPKM 240

Query: 241 IFERHEEYLYLNLVKEIISDGTSKDDRTGTGTLSRFGVQMRYNLRKTFPLLTTKALFWRG 300
           I ERHEEYLYL LV++II++GT+K DRTGTGTLS+FG QMR+NLR  FPLLTTK +FWRG
Sbjct: 241 ISERHEEYLYLKLVQDIIAEGTTKGDRTGTGTLSKFGCQMRFNLRGNFPLLTTKKVFWRG 300

Query: 301 VVEELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDRQLLIHFHNLNFIGLSEREEGDLG 360
           VVEELLWFISGSTNAKVLQEKGIHIWDGNASR+YLD            +GL+EREEGDLG
Sbjct: 301 VVEELLWFISGSTNAKVLQEKGIHIWDGNASREYLDG-----------VGLTEREEGDLG 360

Query: 361 PVYGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKTNPNDRRIILSAWNPSDLKLMAL 420
           PVYGFQWRHFGARYTDMH DY+GQGFDQLLDVINKIK NP+DRRIILSAWNP DLKLMAL
Sbjct: 361 PVYGFQWRHFGARYTDMHHDYSGQGFDQLLDVINKIKRNPDDRRIILSAWNPVDLKLMAL 420

Query: 421 PPCHMFAQVGFKFMVLCSPFISLHYSFIFLQFYVANGELSCQMYQRSADMGLGVPFNIAS 480
           PPCHMFA                       QFYVA+GELSCQMYQRSADMGLG+PFNIAS
Sbjct: 421 PPCHMFA-----------------------QFYVAHGELSCQMYQRSADMGLGIPFNIAS 480

Query: 481 YALLTCMIAHVCDLAQGDFIHVIGDAHVYHTHVRPLQEQLQKLPKPFPILKINPEKKDID 540
           YALLTCMIAHVCDL  GDFIHVIGDAH+Y  HVRPLQEQL   PKPFP LKINP+KKDID
Sbjct: 481 YALLTCMIAHVCDLIPGDFIHVIGDAHIYRNHVRPLQEQLHNQPKPFPTLKINPKKKDID 530

Query: 541 SFIAADFELTGYDPHEKIAMKMAV 563
           SF+AADF+L GYDPH+KI MK++V
Sbjct: 541 SFVAADFKLIGYDPHQKIDMKLSV 530

[Close]

BLAST of MS020549 vs. ExPASy Swiss-Prot
Match: Q05762 (Bifunctional dihydrofolate reductase-thymidylate synthase 1 OS=Arabidopsis thaliana OX=3702 GN=THY-1 PE=1 SV=2)

HSP 1 Score: 828.6 bits (2139), Expect = 4.4e-239
Identity = 397/556 (71.40%), Postives = 459/556 (82.55%), Query Frame = 0


Query: 7   TSSNGNANVIAPPDPRRTFQVVVAATENMGIGKDGKLPWRLPSDLKYFKNITTTTSDHGK 66
           T+ N +       +P+RT+QVVVAAT+ MGIGKDGKLPW LP+DLK+FK+IT TTSD  K
Sbjct: 4   TTLNDSVTTTLASEPQRTYQVVVAATKEMGIGKDGKLPWNLPTDLKFFKDITLTTSDSSK 63

Query: 67  RNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALELLAASP 126
           +NAV+MGRKTWESIP ++RPL GRLNVVLTRSG FDIA  ENV+ C S+ SAL+LLAA P
Sbjct: 64  KNAVVMGRKTWESIPIKYRPLSGRLNVVLTRSGGFDIANTENVVTCSSVDSALDLLAAPP 123

Query: 127 YCLSIEKVFIIGGGEILREALNAPECDAIHVTEIQTCVECDTFIPAIDKSMFQPWYSSFP 186
           YCLSIE+VF+IGGG+ILREALN P CDAIH+TEI T V+CDTFIPAID S++QPW SSFP
Sbjct: 124 YCLSIERVFVIGGGDILREALNRPSCDAIHLTEIDTSVDCDTFIPAIDTSVYQPWSSSFP 183

Query: 187 VVENNLRYSFTSYVRVRSSIEPPVDQNNGLVSYSRPDSSNFEAKKFSFLPKEIFERHEEY 246
           V EN LR+ FT++VRV+SS +   D++NG        S  F+ KKF FLPK +F++HEE+
Sbjct: 184 VTENGLRFCFTTFVRVKSSADESSDESNG------SQSLQFDGKKFLFLPKMVFDQHEEF 243

Query: 247 LYLNLVKEIISDGTSKDDRTGTGTLSRFGVQMRYNLRKTFPLLTTKALFWRGVVEELLWF 306
           LYLN+V++IIS+G  K+DRTGTGTLS+FG QM++NLR++FPLLTTK +FWRGVVEELLWF
Sbjct: 244 LYLNMVEDIISNGNVKNDRTGTGTLSKFGCQMKFNLRRSFPLLTTKRVFWRGVVEELLWF 303

Query: 307 ISGSTNAKVLQEKGIHIWDGNASRDYLDRQLLIHFHNLNFIGLSEREEGDLGPVYGFQWR 366
           ISGSTNAKVLQEKGIHIWDGNASR+YLD            IGL+EREEGDLGPVYGFQWR
Sbjct: 304 ISGSTNAKVLQEKGIHIWDGNASREYLDG-----------IGLTEREEGDLGPVYGFQWR 363

Query: 367 HFGARYTDMHADYTGQGFDQLLDVINKIKTNPNDRRIILSAWNPSDLKLMALPPCHMFAQ 426
           HFGA+YTDMHADYTGQGFDQL+DVI+KIK NP+DRRII+SAWNPSDLKLMALPPCHMFA 
Sbjct: 364 HFGAKYTDMHADYTGQGFDQLVDVIDKIKNNPDDRRIIMSAWNPSDLKLMALPPCHMFA- 423

Query: 427 VGFKFMVLCSPFISLHYSFIFLQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTCMI 486
                                 QFYVA GELSCQMYQRSADMGLGVPFNIASY+LLTCM+
Sbjct: 424 ----------------------QFYVAEGELSCQMYQRSADMGLGVPFNIASYSLLTCML 483

Query: 487 AHVCDLAQGDFIHVIGDAHVYHTHVRPLQEQLQKLPKPFPILKINPEKKDIDSFIAADFE 546
           AHVCDL  GDFIHV+GDAHVY THVRPLQEQL  LPKPFP++KINPEKK IDSF+A+DF+
Sbjct: 484 AHVCDLVPGDFIHVLGDAHVYKTHVRPLQEQLLNLPKPFPVMKINPEKKQIDSFVASDFD 519

Query: 547 LTGYDPHEKIAMKMAV 563
           LTGYDPH+KI MKMAV
Sbjct: 544 LTGYDPHKKIEMKMAV 519

[Close]

BLAST of MS020549 vs. ExPASy Swiss-Prot
Match: P45350 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 827.8 bits (2137), Expect = 7.5e-239
Identity = 398/558 (71.33%), Postives = 461/558 (82.62%), Query Frame = 0


Query: 6   LTSSNGNANVIAPPDPRRTFQVVVAATENMGIGKDGKLPWRLPSDLKYFKNITTTTSDHG 65
           L ++  N + I  PDP+RT+QVVVAAT+NMGIGKDGKLPWRLPSD+K+FK++T TTSD  
Sbjct: 5   LLANPTNGSGITRPDPQRTYQVVVAATQNMGIGKDGKLPWRLPSDMKFFKDVTMTTSDPL 64

Query: 66  KRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALELLAAS 125
           KRNAV+MGRKTWESIP +HRPLPGRLNVVLTRSGSFDIATVENV+ICGS+ SALELLA S
Sbjct: 65  KRNAVIMGRKTWESIPIQHRPLPGRLNVVLTRSGSFDIATVENVVICGSMISALELLAGS 124

Query: 126 PYCLSIEKVFIIGGGEILREALNAPECDAIHVTEIQTCVECDTFIPAIDKSMFQPWYSSF 185
           PYC+S+EKVF+IGGG+I REALNAP CDA+H+TEI+  +ECDTFIP +D+S+FQPWYSSF
Sbjct: 125 PYCVSVEKVFVIGGGQIYREALNAPGCDAVHITEIEEHIECDTFIPLLDESVFQPWYSSF 184

Query: 186 PVVENNLRYSFTSYVRVRSSIEPPVDQNNGLVSYSRPDSSNFEAKKFSFLPKEIFERHEE 245
           P+VEN +RY FT+YVRVR+S+     Q NG  S S+ DS NFE + FSFLPK +FE+HEE
Sbjct: 185 PLVENKIRYCFTTYVRVRNSVAELTSQTNGCSSDSKSDSGNFEIQNFSFLPKTVFEKHEE 244

Query: 246 YLYLNLVKEIISDGTSKDDRTGTGTLSRFGVQMRYNLRKTFPLLTTKALFWRGVVEELLW 305
           YLYL LV+ IIS+G +K+DRT TGT+S FG QMR+NLRK+FPLLTTK +FWRGVVEELLW
Sbjct: 245 YLYLGLVENIISNGVTKNDRTRTGTVSIFGCQMRFNLRKSFPLLTTKKVFWRGVVEELLW 304

Query: 306 FISGSTNAKVLQEKGIHIWDGNASRDYLDRQLLIHFHNLNFIGLSEREEGDLGPVYGFQW 365
           FISGSTNAK+L+EKG++IW+GN SR+YLD            IGL++REEGDLGP+YGFQW
Sbjct: 305 FISGSTNAKILKEKGVNIWEGNGSREYLDS-----------IGLTDREEGDLGPIYGFQW 364

Query: 366 RHFGARYTDMHADYTGQGFDQLLDVINKIKTNPNDRRIILSAWNPSDLKLMALPPCHMFA 425
           RHFGARYTDMHADY+GQGFDQLLDVI+KIK NP+DRRII SAWNPSDL+LMALPPCHMFA
Sbjct: 365 RHFGARYTDMHADYSGQGFDQLLDVISKIKNNPDDRRIIQSAWNPSDLRLMALPPCHMFA 424

Query: 426 QVGFKFMVLCSPFISLHYSFIFLQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTCM 485
                                  QFYVANGELSCQMYQRSADMGLGVPFNIA+YALLTCM
Sbjct: 425 -----------------------QFYVANGELSCQMYQRSADMGLGVPFNIAAYALLTCM 484

Query: 486 IAHVCDLAQGDFIHVIGDAHVYHTHVRPLQE-QLQKLPKPFPILKINPEKKDIDSFIAAD 545
           IAHVCDL  GDF+H IGDAHVY  H+  L E   + LPK FP+LKIN  +KDIDSF AAD
Sbjct: 485 IAHVCDLVPGDFVHSIGDAHVYSNHLSDLFETSFRMLPKTFPVLKINSGEKDIDSFEAAD 528

Query: 546 FELTGYDPHEKIAMKMAV 563
           F+L GYDPH+KI MKMAV
Sbjct: 545 FKLIGYDPHQKIEMKMAV 528

[Close]

BLAST of MS020549 vs. ExPASy Swiss-Prot
Match: O81395 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Zea mays OX=4577 GN=DRTS PE=2 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 8.8e-232
Identity = 382/542 (70.48%), Postives = 443/542 (81.73%), Query Frame = 0


Query: 21  PRRTFQVVVAATENMGIGKDGKLPWRLPSDLKYFKNITTTTSDHGKRNAVLMGRKTWESI 80
           P+R +QVVVA T +MGIGKDG LPW+LP DLK+FK +T TTSD  K+NAV+MGRKTWESI
Sbjct: 14  PQRNYQVVVAGTRDMGIGKDGVLPWKLPGDLKFFKELTLTTSDPVKKNAVIMGRKTWESI 73

Query: 81  PAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALELLAASPYCLSIEKVFIIGGG 140
           P + RPLPGRLNV+LTRSGSFD ATVENV+ICGS+ SALELLA++PYCLSIEKVF+IGGG
Sbjct: 74  PVKSRPLPGRLNVILTRSGSFDFATVENVVICGSMESALELLASTPYCLSIEKVFVIGGG 133

Query: 141 EILREALNAPECDAIHVTEIQTCVECDTFIPAIDKSMFQPWYSSFPVVENNLRYSFTSYV 200
           ++LRE L  P C+AIH+T+IQ+ +ECDTFIP +D S+FQPWYSSFPV+E+N+R+SF S+V
Sbjct: 134 QVLREYLKGPACEAIHLTDIQSSIECDTFIPPVDFSVFQPWYSSFPVIESNIRHSFVSFV 193

Query: 201 RVRSSIEPPVDQNNGLVSYSRPDSSNFEAKKFSFLPKEIFERHEEYLYLNLVKEIISDGT 260
           RVR S+    + N    +     +  FE + FSFLPK +++RHEEY YLNLV++II  G 
Sbjct: 194 RVRKSVAETHESNGKESTEVDTKNDKFETENFSFLPKMVYDRHEEYQYLNLVEDIIRSGA 253

Query: 261 SKDDRTGTGTLSRFGVQMRYNLRKTFPLLTTKALFWRGVVEELLWFISGSTNAKVLQEKG 320
            K+DRTGTGTLS+FG QMR+NLRK FPLLTTK +FWRGVVEELLWFISGSTNAKVLQEKG
Sbjct: 254 QKNDRTGTGTLSKFGCQMRFNLRKNFPLLTTKRVFWRGVVEELLWFISGSTNAKVLQEKG 313

Query: 321 IHIWDGNASRDYLDRQLLIHFHNLNFIGLSEREEGDLGPVYGFQWRHFGARYTDMHADYT 380
           IHIWDGNASR+Y           LN +GL+ REEGDLGP+YGFQWRHFGA YTDMHADYT
Sbjct: 314 IHIWDGNASREY-----------LNSVGLAHREEGDLGPIYGFQWRHFGAEYTDMHADYT 373

Query: 381 GQGFDQLLDVINKIKTNPNDRRIILSAWNPSDLKLMALPPCHMFAQVGFKFMVLCSPFIS 440
           G+GFDQL+DVI+KIK +P DRRIILSAWNPSDLK MALPPCHMFA               
Sbjct: 374 GKGFDQLMDVIDKIKNDPEDRRIILSAWNPSDLKKMALPPCHMFA--------------- 433

Query: 441 LHYSFIFLQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAQGDFIHV 500
                   QFYV NGELSCQMYQRSADMGLGVPFNIASY+LLT MIA VCDL+ GDF+HV
Sbjct: 434 --------QFYVENGELSCQMYQRSADMGLGVPFNIASYSLLTYMIAQVCDLSPGDFVHV 493

Query: 501 IGDAHVYHTHVRPLQEQLQKLPKPFPILKINPEKKDIDSFIAADFELTGYDPHEKIAMKM 560
           IGDAHVY  HVR L+EQ+QK+PKPFPILKINP KKDIDSF+A+DF+L GYDPH+KI MKM
Sbjct: 494 IGDAHVYRNHVRALEEQIQKMPKPFPILKINPSKKDIDSFMASDFKLVGYDPHQKIEMKM 521

Query: 561 AV 563
           AV
Sbjct: 554 AV 521

[Close]

BLAST of MS020549 vs. ExPASy Swiss-Prot
Match: Q05763 (Bifunctional dihydrofolate reductase-thymidylate synthase 2 OS=Arabidopsis thaliana OX=3702 GN=THY-2 PE=2 SV=2)

HSP 1 Score: 803.5 bits (2074), Expect = 1.5e-231
Identity = 393/551 (71.32%), Postives = 442/551 (80.22%), Query Frame = 0


Query: 12  NANVIAPPDPRRTFQVVVAATENMGIGKDGKLPWRLPSDLKYFKNITTTTSDHGKRNAVL 71
           N NVI    P+ T+QVVVAAT+ MGIGKDGKLPW LP+DLK+FK++T +TSD  K+NAV+
Sbjct: 53  NGNVIMTSKPQSTYQVVVAATKEMGIGKDGKLPWNLPTDLKFFKDLTLSTSDSAKKNAVV 112

Query: 72  MGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALELLAASPYCLSI 131
           MGRKTWESIP ++RPL GRLNVVL+RS  FDIA  ENV+ C SI SAL+LLAA P+ LSI
Sbjct: 113 MGRKTWESIPKKYRPLSGRLNVVLSRSSGFDIANTENVVTCSSIDSALDLLAAPPFSLSI 172

Query: 132 EKVFIIGGGEILREALNAPECDAIHVTEIQTCVECDTFIPAIDKSMFQPWYSSFPVVENN 191
           EKVF+IGGG+ILREALN P C+AIH+TEI T ++CDTFIP +D S +QPW SSFP+ EN 
Sbjct: 173 EKVFVIGGGDILREALNKPSCEAIHITEIDTSIDCDTFIPTVDTSAYQPWCSSFPICENG 232

Query: 192 LRYSFTSYVRVRSSIEPPVDQNNGLVSYSRPDSSNFEAKKFSFLPKEIFERHEEYLYLNL 251
           LR+SFT++VRV+SS        +      + D   F     S LPK IF+RHEEYLYLNL
Sbjct: 233 LRFSFTTHVRVKSSSAGEASDESDGSKVLQVDWKKFS----SVLPKMIFDRHEEYLYLNL 292

Query: 252 VKEIISDGTSKDDRTGTGTLSRFGVQMRYNLRKTFPLLTTKALFWRGVVEELLWFISGST 311
           VKEIIS+G  KDDRTGTGTLS+FG QM++NLR+ FPLLTTK +FWRGVVEELLWFISGST
Sbjct: 293 VKEIISNGNLKDDRTGTGTLSKFGCQMKFNLRRNFPLLTTKRVFWRGVVEELLWFISGST 352

Query: 312 NAKVLQEKGIHIWDGNASRDYLDRQLLIHFHNLNFIGLSEREEGDLGPVYGFQWRHFGAR 371
           NAKVLQEKGI IWDGNASR YLD            IGL+EREEGDLGPVYGFQWRHFGA+
Sbjct: 353 NAKVLQEKGIRIWDGNASRAYLDG-----------IGLTEREEGDLGPVYGFQWRHFGAK 412

Query: 372 YTDMHADYTGQGFDQLLDVINKIKTNPNDRRIILSAWNPSDLKLMALPPCHMFAQVGFKF 431
           YTDMHADYTGQGFDQLLDVINKIK NP+DRRII+SAWNPSDLKLMALPPCHMFA      
Sbjct: 413 YTDMHADYTGQGFDQLLDVINKIKNNPDDRRIIMSAWNPSDLKLMALPPCHMFA------ 472

Query: 432 MVLCSPFISLHYSFIFLQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCD 491
                            QFYVANGELSCQMYQRSADMGLGVPFNIASY+LLTC++AHVCD
Sbjct: 473 -----------------QFYVANGELSCQMYQRSADMGLGVPFNIASYSLLTCILAHVCD 532

Query: 492 LAQGDFIHVIGDAHVYHTHVRPLQEQLQKLPKPFPILKINPEKKDIDSFIAADFELTGYD 551
           L  GDFIHVIGDAHVY  HVRPLQEQL+  PKPFP+LKINPEKKDIDSF+A DFEL GYD
Sbjct: 533 LVPGDFIHVIGDAHVYKNHVRPLQEQLENPPKPFPVLKINPEKKDIDSFVADDFELIGYD 565

Query: 552 PHEKIAMKMAV 563
           PH+KI MKMAV
Sbjct: 593 PHKKIDMKMAV 565

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BLAST of MS020549 vs. ExPASy TrEMBL
Match: A0A6J1CYC5 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Momordica charantia OX=3673 GN=LOC111015400 PE=3 SV=1)

HSP 1 Score: 1059.7 bits (2739), Expect = 4.3e-306
Identity = 523/562 (93.06%), Postives = 526/562 (93.59%), Query Frame = 0


Query: 1   MAGDPLTSSNGNANVIAPPDPRRTFQVVVAATENMGIGKDGKLPWRLPSDLKYFKNITTT 60
           MAGDPLTSSNGNANVIAPPDPRRTFQVVVAATENMGIGKDGKLPWRLPSDLKYFKNITTT
Sbjct: 10  MAGDPLTSSNGNANVIAPPDPRRTFQVVVAATENMGIGKDGKLPWRLPSDLKYFKNITTT 69

Query: 61  TSDHGKRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE 120
           TSDHGKRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE
Sbjct: 70  TSDHGKRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE 129

Query: 121 LLAASPYCLSIEKVFIIGGGEILREALNAPECDAIHVTEIQTCVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVFIIGGGEILREALNAPECDAIHVT+IQTCVECDTFIPAIDKSMFQP
Sbjct: 130 LLAASPYCLSIEKVFIIGGGEILREALNAPECDAIHVTDIQTCVECDTFIPAIDKSMFQP 189

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSIEPPVDQNNGLVSYSRPDSSNFEAKKFSFLPKEIF 240
           WYSSFPVVENNLRYSFTSYVRVRSSIEPP+DQNNGLVSYSRPDSSNFEAKKFSFLPKEIF
Sbjct: 190 WYSSFPVVENNLRYSFTSYVRVRSSIEPPIDQNNGLVSYSRPDSSNFEAKKFSFLPKEIF 249

Query: 241 ERHEEYLYLNLVKEIISDGTSKDDRTGTGTLSRFGVQMRYNLRKTFPLLTTKALFWRGVV 300
           ERHEEYLYLNLVKEIISDGTSKDDRTGTGTLSRFGVQMRYNLRKTFPLLTTK +FWRGVV
Sbjct: 250 ERHEEYLYLNLVKEIISDGTSKDDRTGTGTLSRFGVQMRYNLRKTFPLLTTKKVFWRGVV 309

Query: 301 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDRQLLIHFHNLNFIGLSEREEGDLGPV 360
           EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLD            IGLSEREEGDLGPV
Sbjct: 310 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDS-----------IGLSEREEGDLGPV 369

Query: 361 YGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKTNPNDRRIILSAWNPSDLKLMALPP 420
           YGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKTNPNDRRIILSAWNPSDLKLMALPP
Sbjct: 370 YGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKTNPNDRRIILSAWNPSDLKLMALPP 429

Query: 421 CHMFAQVGFKFMVLCSPFISLHYSFIFLQFYVANGELSCQMYQRSADMGLGVPFNIASYA 480
           CHMFA                       QFYVANGELSCQMYQRSADMGLGVPFNIASYA
Sbjct: 430 CHMFA-----------------------QFYVANGELSCQMYQRSADMGLGVPFNIASYA 489

Query: 481 LLTCMIAHVCDLAQGDFIHVIGDAHVYHTHVRPLQEQLQKLPKPFPILKINPEKKDIDSF 540
           LLTCMIAHVCDLAQGDFIHVIGDAHVYHTHVRPLQEQLQKLPKPFPILKINPEKKDIDSF
Sbjct: 490 LLTCMIAHVCDLAQGDFIHVIGDAHVYHTHVRPLQEQLQKLPKPFPILKINPEKKDIDSF 537

Query: 541 IAADFELTGYDPHEKIAMKMAV 563
           IAADFELTGYDPHEKIAMKMAV
Sbjct: 550 IAADFELTGYDPHEKIAMKMAV 537

[Close]

BLAST of MS020549 vs. ExPASy TrEMBL
Match: A0A6J1FWP8 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucurbita moschata OX=3662 GN=LOC111448895 PE=3 SV=1)

HSP 1 Score: 1008.1 bits (2605), Expect = 1.5e-290
Identity = 492/562 (87.54%), Postives = 513/562 (91.28%), Query Frame = 0


Query: 1   MAGDPLTSSNGNANVIAPPDPRRTFQVVVAATENMGIGKDGKLPWRLPSDLKYFKNITTT 60
           MA DPLTSSNGNANV APP+PRRT+QVVVAATENMGIGKDGKLPWRLPSDLK+FKNIT T
Sbjct: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITAT 60

Query: 61  TSDHGKRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE 120
           TSDHGKRNA+LMGRKTWESIPAE+RPLPGRLNVVLTRSGSFDIATVENVIICGS+TSALE
Sbjct: 61  TSDHGKRNAILMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120

Query: 121 LLAASPYCLSIEKVFIIGGGEILREALNAPECDAIHVTEIQTCVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVF+IGGGEILREALNAPECDA+HVTEI+T VECDTFIPAIDKSMFQP
Sbjct: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSIEPPVDQNNGLVSYSRPDSSNFEAKKFSFLPKEIF 240
           WYSSFPVVENNLRYSFTSYVRVRSS EPP+DQNNGLVS+++PDS NFEAK FSFLPKEIF
Sbjct: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEIF 240

Query: 241 ERHEEYLYLNLVKEIISDGTSKDDRTGTGTLSRFGVQMRYNLRKTFPLLTTKALFWRGVV 300
           ERHEEYLYL LV+EIISDGTSKDDRTGTGTLS+FG QMRYNLR+TFPLLTTK +FWRGVV
Sbjct: 241 ERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRRTFPLLTTKKVFWRGVV 300

Query: 301 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDRQLLIHFHNLNFIGLSEREEGDLGPV 360
           EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLD            IGL EREEGDLGPV
Sbjct: 301 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDS-----------IGLREREEGDLGPV 360

Query: 361 YGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKTNPNDRRIILSAWNPSDLKLMALPP 420
           YGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIK NP+DRRI+LSAWNPSDLKLMALPP
Sbjct: 361 YGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPP 420

Query: 421 CHMFAQVGFKFMVLCSPFISLHYSFIFLQFYVANGELSCQMYQRSADMGLGVPFNIASYA 480
           CHMFA                       QFYVANGELSCQMYQRSADMGLGVPFNIASYA
Sbjct: 421 CHMFA-----------------------QFYVANGELSCQMYQRSADMGLGVPFNIASYA 480

Query: 481 LLTCMIAHVCDLAQGDFIHVIGDAHVYHTHVRPLQEQLQKLPKPFPILKINPEKKDIDSF 540
           LLTCMIAHVCDLA GDFIHVIGDAHVY THVRPLQEQLQKLPKPFP+LKINPEKKDIDSF
Sbjct: 481 LLTCMIAHVCDLAYGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPVLKINPEKKDIDSF 528

Query: 541 IAADFELTGYDPHEKIAMKMAV 563
           +AADFELTGYDPHEKIAMKMAV
Sbjct: 541 VAADFELTGYDPHEKIAMKMAV 528

[Close]

BLAST of MS020549 vs. ExPASy TrEMBL
Match: A0A6J1J7P4 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucurbita maxima OX=3661 GN=LOC111484218 PE=3 SV=1)

HSP 1 Score: 1000.7 bits (2586), Expect = 2.4e-288
Identity = 491/562 (87.37%), Postives = 511/562 (90.93%), Query Frame = 0


Query: 1   MAGDPLTSSNGNANVIAPPDPRRTFQVVVAATENMGIGKDGKLPWRLPSDLKYFKNITTT 60
           MA DPLTSSNGNANV APP+PRRT+QVVVAATENMGIGKDGKLPWRLPSDLK+FKNITTT
Sbjct: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTT 60

Query: 61  TSDHGKRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE 120
           TSDHGKRNAVLMGRKTWESIPAE+RPLPGRLNVVLTRSGSFDIATVENVIICGS+TSALE
Sbjct: 61  TSDHGKRNAVLMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120

Query: 121 LLAASPYCLSIEKVFIIGGGEILREALNAPECDAIHVTEIQTCVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVF+IGGGEILREALNAPECDA+HVTEI+T VECDTFIPAIDKSMFQP
Sbjct: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSIEPPVDQNNGLVSYSRPDSSNFEAKKFSFLPKEIF 240
           WYSSFP+VENNLRYSFTSYVRVRSS EPP+DQNNGLVS+++PDS N   K FSFLPKEIF
Sbjct: 181 WYSSFPMVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFN---KNFSFLPKEIF 240

Query: 241 ERHEEYLYLNLVKEIISDGTSKDDRTGTGTLSRFGVQMRYNLRKTFPLLTTKALFWRGVV 300
           ERHEEYLYL LV+EIISDGTSKDDRTGTGTLS+FG QMRYNLRKTFPLLTTK +FWRGVV
Sbjct: 241 ERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTKKVFWRGVV 300

Query: 301 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDRQLLIHFHNLNFIGLSEREEGDLGPV 360
           EELLWFISGSTNAK+LQEKGIHIWDGNASRDYLD            IGL EREEGDLGPV
Sbjct: 301 EELLWFISGSTNAKILQEKGIHIWDGNASRDYLDS-----------IGLREREEGDLGPV 360

Query: 361 YGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKTNPNDRRIILSAWNPSDLKLMALPP 420
           YGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIK NPNDRRI+LSAWNPSDLKLMALPP
Sbjct: 361 YGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKNNPNDRRIVLSAWNPSDLKLMALPP 420

Query: 421 CHMFAQVGFKFMVLCSPFISLHYSFIFLQFYVANGELSCQMYQRSADMGLGVPFNIASYA 480
           CHMFA                       QFYVANGELSCQMYQRSADMGLGVPFNIASYA
Sbjct: 421 CHMFA-----------------------QFYVANGELSCQMYQRSADMGLGVPFNIASYA 480

Query: 481 LLTCMIAHVCDLAQGDFIHVIGDAHVYHTHVRPLQEQLQKLPKPFPILKINPEKKDIDSF 540
           LLTCMIAHVCDLA GDFIHVIGDAHVY THVRPLQEQLQKLPKPFP+LKINPEKKDIDSF
Sbjct: 481 LLTCMIAHVCDLAYGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPVLKINPEKKDIDSF 525

Query: 541 IAADFELTGYDPHEKIAMKMAV 563
           +AADFELTGYDPHEKIAMKMAV
Sbjct: 541 VAADFELTGYDPHEKIAMKMAV 525

[Close]

BLAST of MS020549 vs. ExPASy TrEMBL
Match: A0A5A7TVM8 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold243G00740 PE=3 SV=1)

HSP 1 Score: 993.4 bits (2567), Expect = 3.8e-286
Identity = 489/571 (85.64%), Postives = 513/571 (89.84%), Query Frame = 0


Query: 1   MAGDPLTSSNGNANVIAPPDPRRTFQVVVAATENMGIGKDGKLPWRLPSDLKYFKNITTT 60
           MA DPLT SNG ANV + PDPRRT+QVVVAATEN+GIGKDGKLPWRLPSDLK+FKNITTT
Sbjct: 49  MAADPLTISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTT 108

Query: 61  TSDHGKRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE 120
           TSD GKRNA+LMGRKTWES+PAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSI SALE
Sbjct: 109 TSDPGKRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALE 168

Query: 121 LLAASPYCLSIEKVFIIGGGEILREALNAPECDAIHVTEIQTCVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVF+IGGGEILREA+NAPECDA+H+TEI+T +ECDTFIPAIDK+MFQP
Sbjct: 169 LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDKAMFQP 228

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSIEPPVDQNNGLVSYSRPDSSNFEAKKFSFLPKEIF 240
           WYSSFPVVENNLRYSF SYVRVRSS E P+DQNNGLVS+++PD SN EAKKFSFLPKEIF
Sbjct: 229 WYSSFPVVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIF 288

Query: 241 ERHEEYLYLNLVKEIISDGTSKDDRTGTGTLSRFGVQMRYNLRKTFPLLTTKALFWRGVV 300
           ERHEEYLYL LV+EIISDG  KDDRTGTGTLS+FG QMRYNLRKTFPLLTTKALFWRGVV
Sbjct: 289 ERHEEYLYLKLVQEIISDGIPKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTKALFWRGVV 348

Query: 301 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDRQLLIHFHNLNF---------IGLSE 360
           EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDRQLLIH  N +F         IGL E
Sbjct: 349 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDRQLLIHL-NFDFLNKKLVKLDIGLRE 408

Query: 361 REEGDLGPVYGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKTNPNDRRIILSAWNPS 420
           REEGDLGPVYGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIK NP+DRRIILSAWNPS
Sbjct: 409 REEGDLGPVYGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPS 468

Query: 421 DLKLMALPPCHMFAQVGFKFMVLCSPFISLHYSFIFLQFYVANGELSCQMYQRSADMGLG 480
           DLKLMALPPCHMF+                       QFYVANGELSCQMYQRSADMGLG
Sbjct: 469 DLKLMALPPCHMFS-----------------------QFYVANGELSCQMYQRSADMGLG 528

Query: 481 VPFNIASYALLTCMIAHVCDLAQGDFIHVIGDAHVYHTHVRPLQEQLQKLPKPFPILKIN 540
           VPFNIASYALLTCMIAHVC LA+GDFIHVIGDAHVY THVRPLQEQLQKLPKPFPILKIN
Sbjct: 529 VPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPILKIN 588

Query: 541 PEKKDIDSFIAADFELTGYDPHEKIAMKMAV 563
           PEKKDIDSF+A DFELTGYDPH KIAMKMAV
Sbjct: 589 PEKKDIDSFVADDFELTGYDPHVKIAMKMAV 595

[Close]

BLAST of MS020549 vs. ExPASy TrEMBL
Match: A0A1S3CE37 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucumis melo OX=3656 GN=LOC103499691 PE=3 SV=1)

HSP 1 Score: 977.2 bits (2525), Expect = 2.8e-281
Identity = 479/562 (85.23%), Postives = 503/562 (89.50%), Query Frame = 0


Query: 1   MAGDPLTSSNGNANVIAPPDPRRTFQVVVAATENMGIGKDGKLPWRLPSDLKYFKNITTT 60
           MA DPLT SNG ANV + PDPRRT+QVVVAATEN+GIGKDGKLPWRLPSDLK+FKNITTT
Sbjct: 1   MAADPLTISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTT 60

Query: 61  TSDHGKRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE 120
           TSD GKRNA+LMGRKTWES+PAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSI SALE
Sbjct: 61  TSDPGKRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALE 120

Query: 121 LLAASPYCLSIEKVFIIGGGEILREALNAPECDAIHVTEIQTCVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVF+IGGGEILREA+NAPECDA+H+TEI+T +ECDTFIPAIDK+MFQP
Sbjct: 121 LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDKAMFQP 180

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSIEPPVDQNNGLVSYSRPDSSNFEAKKFSFLPKEIF 240
           WYSSFPVVENNLRYSF SYVRVRSS E P+DQNNGLVS+++PD SN EAKKFSFLPKEIF
Sbjct: 181 WYSSFPVVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIF 240

Query: 241 ERHEEYLYLNLVKEIISDGTSKDDRTGTGTLSRFGVQMRYNLRKTFPLLTTKALFWRGVV 300
           ERHEEYLYL LV+EIISDG  KDDRTGTGTLS+FG QMRYNLRKTFPLLTTK +FWRGVV
Sbjct: 241 ERHEEYLYLKLVQEIISDGIPKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTKKVFWRGVV 300

Query: 301 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDRQLLIHFHNLNFIGLSEREEGDLGPV 360
           EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLD            IGL EREEGDLGPV
Sbjct: 301 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDS-----------IGLREREEGDLGPV 360

Query: 361 YGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKTNPNDRRIILSAWNPSDLKLMALPP 420
           YGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIK NP+DRRIILSAWNPSDLKLMALPP
Sbjct: 361 YGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPP 420

Query: 421 CHMFAQVGFKFMVLCSPFISLHYSFIFLQFYVANGELSCQMYQRSADMGLGVPFNIASYA 480
           CHMF+                       QFYVANGELSCQMYQRSADMGLGVPFNIASYA
Sbjct: 421 CHMFS-----------------------QFYVANGELSCQMYQRSADMGLGVPFNIASYA 480

Query: 481 LLTCMIAHVCDLAQGDFIHVIGDAHVYHTHVRPLQEQLQKLPKPFPILKINPEKKDIDSF 540
           LLTCMIAHVC LA+GDFIHVIGDAHVY THVRPLQEQLQKLPKPFPILKINPEKKDIDSF
Sbjct: 481 LLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPILKINPEKKDIDSF 528

Query: 541 IAADFELTGYDPHEKIAMKMAV 563
           +A DFELTGYDPH KIAMKMAV
Sbjct: 541 VADDFELTGYDPHVKIAMKMAV 528

[Close]

BLAST of MS020549 vs. TAIR 10
Match: AT2G16370.1 (thymidylate synthase 1 )

HSP 1 Score: 828.6 bits (2139), Expect = 3.1e-240
Identity = 397/556 (71.40%), Postives = 459/556 (82.55%), Query Frame = 0


Query: 7   TSSNGNANVIAPPDPRRTFQVVVAATENMGIGKDGKLPWRLPSDLKYFKNITTTTSDHGK 66
           T+ N +       +P+RT+QVVVAAT+ MGIGKDGKLPW LP+DLK+FK+IT TTSD  K
Sbjct: 4   TTLNDSVTTTLASEPQRTYQVVVAATKEMGIGKDGKLPWNLPTDLKFFKDITLTTSDSSK 63

Query: 67  RNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALELLAASP 126
           +NAV+MGRKTWESIP ++RPL GRLNVVLTRSG FDIA  ENV+ C S+ SAL+LLAA P
Sbjct: 64  KNAVVMGRKTWESIPIKYRPLSGRLNVVLTRSGGFDIANTENVVTCSSVDSALDLLAAPP 123

Query: 127 YCLSIEKVFIIGGGEILREALNAPECDAIHVTEIQTCVECDTFIPAIDKSMFQPWYSSFP 186
           YCLSIE+VF+IGGG+ILREALN P CDAIH+TEI T V+CDTFIPAID S++QPW SSFP
Sbjct: 124 YCLSIERVFVIGGGDILREALNRPSCDAIHLTEIDTSVDCDTFIPAIDTSVYQPWSSSFP 183

Query: 187 VVENNLRYSFTSYVRVRSSIEPPVDQNNGLVSYSRPDSSNFEAKKFSFLPKEIFERHEEY 246
           V EN LR+ FT++VRV+SS +   D++NG        S  F+ KKF FLPK +F++HEE+
Sbjct: 184 VTENGLRFCFTTFVRVKSSADESSDESNG------SQSLQFDGKKFLFLPKMVFDQHEEF 243

Query: 247 LYLNLVKEIISDGTSKDDRTGTGTLSRFGVQMRYNLRKTFPLLTTKALFWRGVVEELLWF 306
           LYLN+V++IIS+G  K+DRTGTGTLS+FG QM++NLR++FPLLTTK +FWRGVVEELLWF
Sbjct: 244 LYLNMVEDIISNGNVKNDRTGTGTLSKFGCQMKFNLRRSFPLLTTKRVFWRGVVEELLWF 303

Query: 307 ISGSTNAKVLQEKGIHIWDGNASRDYLDRQLLIHFHNLNFIGLSEREEGDLGPVYGFQWR 366
           ISGSTNAKVLQEKGIHIWDGNASR+YLD            IGL+EREEGDLGPVYGFQWR
Sbjct: 304 ISGSTNAKVLQEKGIHIWDGNASREYLDG-----------IGLTEREEGDLGPVYGFQWR 363

Query: 367 HFGARYTDMHADYTGQGFDQLLDVINKIKTNPNDRRIILSAWNPSDLKLMALPPCHMFAQ 426
           HFGA+YTDMHADYTGQGFDQL+DVI+KIK NP+DRRII+SAWNPSDLKLMALPPCHMFA 
Sbjct: 364 HFGAKYTDMHADYTGQGFDQLVDVIDKIKNNPDDRRIIMSAWNPSDLKLMALPPCHMFA- 423

Query: 427 VGFKFMVLCSPFISLHYSFIFLQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTCMI 486
                                 QFYVA GELSCQMYQRSADMGLGVPFNIASY+LLTCM+
Sbjct: 424 ----------------------QFYVAEGELSCQMYQRSADMGLGVPFNIASYSLLTCML 483

Query: 487 AHVCDLAQGDFIHVIGDAHVYHTHVRPLQEQLQKLPKPFPILKINPEKKDIDSFIAADFE 546
           AHVCDL  GDFIHV+GDAHVY THVRPLQEQL  LPKPFP++KINPEKK IDSF+A+DF+
Sbjct: 484 AHVCDLVPGDFIHVLGDAHVYKTHVRPLQEQLLNLPKPFPVMKINPEKKQIDSFVASDFD 519

Query: 547 LTGYDPHEKIAMKMAV 563
           LTGYDPH+KI MKMAV
Sbjct: 544 LTGYDPHKKIEMKMAV 519

[Close]

BLAST of MS020549 vs. TAIR 10
Match: AT4G34570.1 (thymidylate synthase 2 )

HSP 1 Score: 803.5 bits (2074), Expect = 1.1e-232
Identity = 393/551 (71.32%), Postives = 442/551 (80.22%), Query Frame = 0


Query: 12  NANVIAPPDPRRTFQVVVAATENMGIGKDGKLPWRLPSDLKYFKNITTTTSDHGKRNAVL 71
           N NVI    P+ T+QVVVAAT+ MGIGKDGKLPW LP+DLK+FK++T +TSD  K+NAV+
Sbjct: 53  NGNVIMTSKPQSTYQVVVAATKEMGIGKDGKLPWNLPTDLKFFKDLTLSTSDSAKKNAVV 112

Query: 72  MGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALELLAASPYCLSI 131
           MGRKTWESIP ++RPL GRLNVVL+RS  FDIA  ENV+ C SI SAL+LLAA P+ LSI
Sbjct: 113 MGRKTWESIPKKYRPLSGRLNVVLSRSSGFDIANTENVVTCSSIDSALDLLAAPPFSLSI 172

Query: 132 EKVFIIGGGEILREALNAPECDAIHVTEIQTCVECDTFIPAIDKSMFQPWYSSFPVVENN 191
           EKVF+IGGG+ILREALN P C+AIH+TEI T ++CDTFIP +D S +QPW SSFP+ EN 
Sbjct: 173 EKVFVIGGGDILREALNKPSCEAIHITEIDTSIDCDTFIPTVDTSAYQPWCSSFPICENG 232

Query: 192 LRYSFTSYVRVRSSIEPPVDQNNGLVSYSRPDSSNFEAKKFSFLPKEIFERHEEYLYLNL 251
           LR+SFT++VRV+SS        +      + D   F     S LPK IF+RHEEYLYLNL
Sbjct: 233 LRFSFTTHVRVKSSSAGEASDESDGSKVLQVDWKKFS----SVLPKMIFDRHEEYLYLNL 292

Query: 252 VKEIISDGTSKDDRTGTGTLSRFGVQMRYNLRKTFPLLTTKALFWRGVVEELLWFISGST 311
           VKEIIS+G  KDDRTGTGTLS+FG QM++NLR+ FPLLTTK +FWRGVVEELLWFISGST
Sbjct: 293 VKEIISNGNLKDDRTGTGTLSKFGCQMKFNLRRNFPLLTTKRVFWRGVVEELLWFISGST 352

Query: 312 NAKVLQEKGIHIWDGNASRDYLDRQLLIHFHNLNFIGLSEREEGDLGPVYGFQWRHFGAR 371
           NAKVLQEKGI IWDGNASR YLD            IGL+EREEGDLGPVYGFQWRHFGA+
Sbjct: 353 NAKVLQEKGIRIWDGNASRAYLDG-----------IGLTEREEGDLGPVYGFQWRHFGAK 412

Query: 372 YTDMHADYTGQGFDQLLDVINKIKTNPNDRRIILSAWNPSDLKLMALPPCHMFAQVGFKF 431
           YTDMHADYTGQGFDQLLDVINKIK NP+DRRII+SAWNPSDLKLMALPPCHMFA      
Sbjct: 413 YTDMHADYTGQGFDQLLDVINKIKNNPDDRRIIMSAWNPSDLKLMALPPCHMFA------ 472

Query: 432 MVLCSPFISLHYSFIFLQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCD 491
                            QFYVANGELSCQMYQRSADMGLGVPFNIASY+LLTC++AHVCD
Sbjct: 473 -----------------QFYVANGELSCQMYQRSADMGLGVPFNIASYSLLTCILAHVCD 532

Query: 492 LAQGDFIHVIGDAHVYHTHVRPLQEQLQKLPKPFPILKINPEKKDIDSFIAADFELTGYD 551
           L  GDFIHVIGDAHVY  HVRPLQEQL+  PKPFP+LKINPEKKDIDSF+A DFEL GYD
Sbjct: 533 LVPGDFIHVIGDAHVYKNHVRPLQEQLENPPKPFPVLKINPEKKDIDSFVADDFELIGYD 565

Query: 552 PHEKIAMKMAV 563
           PH+KI MKMAV
Sbjct: 593 PHKKIDMKMAV 565

[Close]

BLAST of MS020549 vs. TAIR 10
Match: AT2G21550.1 (Bifunctional dihydrofolate reductase/thymidylate synthase )

HSP 1 Score: 550.1 bits (1416), Expect = 2.1e-156
Identity = 283/526 (53.80%), Postives = 362/526 (68.82%), Query Frame = 0


Query: 22  RRTFQVVVAATENMGIGKDGKLPWRLPSDLKYFKNITTTTSDHGKRNAVLMGRKTWESIP 81
           RR++QVV+AAT +MG+G D KLPW LPS+ ++F+++TT TSD  KRNA +MGRK+WES P
Sbjct: 21  RRSYQVVIAATRDMGLGMDMKLPWDLPSEYQFFQDVTTRTSDPTKRNATIMGRKSWESTP 80

Query: 82  AEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALELLAASPYCLSIEKVFIIGGGE 141
            E RPLPGRLN+VLT+S   +IA  ENV++  S+ SALELLA  PY LSIEKVF+IGGGE
Sbjct: 81  LEIRPLPGRLNIVLTKSSCHNIAIDENVLVSSSMESALELLATEPYSLSIEKVFVIGGGE 140

Query: 142 ILREALNAPECDAIHVTEIQTCVECDTFIPAIDKSMFQPWYSSFPVVENNLRYSFTSYVR 201
           +LR  +NA  CDAIH+TEI   V CD F P +D S+++PWYSSFPVVEN +RYSF +YVR
Sbjct: 141 LLRNYMNASICDAIHLTEIDISVPCDAFAPRVDTSLYRPWYSSFPVVENGIRYSFNTYVR 200

Query: 202 VRSSIEPPVDQNNGLVSYSRPDSSNFEAKKFSFLPKEIFERHEEYLYLNLVKEIISDGTS 261
            + +I         + S  +   +  + K++SFLPK +FERHEE+ YLNLV+ IIS G  
Sbjct: 201 RKDAI---------VGSGEKKSVAESDLKEYSFLPKMVFERHEEFGYLNLVQNIISSGDM 260

Query: 262 KDDRTGTGTLSRFGVQMRYNLRKTFPLLTTKALFWRGVVEELLWFISGSTNAKVLQEKGI 321
            D+     TLS+FG QMR+NLRKTFPLLTTK +FW GVVEE+L  ISGS N K   E G 
Sbjct: 261 NDN----STLSKFGCQMRFNLRKTFPLLTTKKIFWLGVVEEILQLISGSNNPK---ENGS 320

Query: 322 HIWDGNASRDYLDRQLLIHFHNLNFIGLSEREEGDLGPVYGFQWRHFGARYTDMHADYTG 381
           HIWD + +++YLD          +F   +  E+GD   ++G  W+H  AR+         
Sbjct: 321 HIWDTDEAKEYLD----------SFGVNATEEDGDNPFLHGLHWKHCDARFVI------- 380

Query: 382 QGFDQLLDVINKIKTNPNDRRIILSAWNPSDLKLMALPPCHMFAQVGFKFMVLCSPFISL 441
           Q F QL DVINKIK NP+D+RI+L+A NP D KL ++ PCH F                 
Sbjct: 381 QEFSQLSDVINKIKNNPHDQRIMLAACNPLDFKL-SVSPCHTFT---------------- 440

Query: 442 HYSFIFLQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAQGDFIHVI 501
                  QFYVANGE+SCQ+YQ S +  +G+PF+IA+Y+LLTC+IAHVCDL  GDFIHVI
Sbjct: 441 -------QFYVANGEVSCQIYQSSTEASIGIPFSIATYSLLTCIIAHVCDLGAGDFIHVI 489

Query: 502 GDAHVYHTHVRPLQEQLQKLPKPFPILKINPEKKDIDSFIAADFEL 548
           G A++   HV+ +Q+QLQ  PKPFPILKINPEKK +D+F A+D EL
Sbjct: 501 GQAYINKAHVKAIQKQLQISPKPFPILKINPEKKKMDNFEASDLEL 489

The following BLAST results are available for this feature:

BLAST of MS020549 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146106.1	8.9e-306	93.06	bifunctional dihydrofolate reductase-thymidylate synthase 1-like [Momordica char...	[more]
XP_022944443.1	3.1e-290	87.54	bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita moscha...	[more]
XP_023513067.1	3.4e-289	87.74	bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita pepo s...	[more]
XP_038901596.1	4.4e-289	87.19	bifunctional dihydrofolate reductase-thymidylate synthase 1-like [Benincasa hisp...	[more]
XP_022986497.1	4.9e-288	87.37	bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita maxima...	[more]

BLAST of MS020549 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P51820	8.0e-241	72.34	Bifunctional dihydrofolate reductase-thymidylate synthase OS=Glycine max OX=3847...	[more]
Q05762	4.4e-239	71.40	Bifunctional dihydrofolate reductase-thymidylate synthase 1 OS=Arabidopsis thali...	[more]
P45350	7.5e-239	71.33	Bifunctional dihydrofolate reductase-thymidylate synthase OS=Daucus carota OX=40...	[more]
O81395	8.8e-232	70.48	Bifunctional dihydrofolate reductase-thymidylate synthase OS=Zea mays OX=4577 GN...	[more]
Q05763	1.5e-231	71.32	Bifunctional dihydrofolate reductase-thymidylate synthase 2 OS=Arabidopsis thali...	[more]

BLAST of MS020549 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CYC5	4.3e-306	93.06	Bifunctional dihydrofolate reductase-thymidylate synthase OS=Momordica charantia...	[more]
A0A6J1FWP8	1.5e-290	87.54	Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucurbita moschata ...	[more]
A0A6J1J7P4	2.4e-288	87.37	Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucurbita maxima OX...	[more]
A0A5A7TVM8	3.8e-286	85.64	Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucumis melo var. m...	[more]
A0A1S3CE37	2.8e-281	85.23	Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucumis melo OX=365...	[more]

BLAST of MS020549 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G16370.1	3.1e-240	71.40	thymidylate synthase 1	[more]
AT4G34570.1	1.1e-232	71.32	thymidylate synthase 2	[more]
AT2G21550.1	2.1e-156	53.80	Bifunctional dihydrofolate reductase/thymidylate synthase	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000398	Thymidylate synthase	PRINTS	PR00108	THYMDSNTHASE	coord: 439..454 score: 35.9 coord: 286..307 score: 72.53 coord: 457..483 score: 83.61 coord: 495..512 score: 57.8
IPR000398	Thymidylate synthase	TIGRFAM	TIGR03284	TIGR03284	coord: 248..426 e-value: 1.3E-54 score: 183.3 coord: 444..562 e-value: 1.6E-45 score: 153.5
IPR000398	Thymidylate synthase	HAMAP	MF_00008	Thymidy_synth_bact	coord: 245..562 score: 34.574921
IPR024072	Dihydrofolate reductase-like domain superfamily	GENE3D	3.40.430.10	Dihydrofolate Reductase, subunit A	coord: 10..203 e-value: 8.8E-64 score: 217.0
IPR024072	Dihydrofolate reductase-like domain superfamily	SUPERFAMILY	53597	Dihydrofolate reductase-like	coord: 22..201
IPR036926	Thymidylate synthase/dCMP hydroxymethylase superfamily	GENE3D	3.30.572.10	Thymidylate synthase/dCMP hydroxymethylase domain	coord: 233..434 e-value: 2.0E-79 score: 268.5
IPR036926	Thymidylate synthase/dCMP hydroxymethylase superfamily	GENE3D	3.30.572.10	Thymidylate synthase/dCMP hydroxymethylase domain	coord: 437..562 e-value: 8.3E-54 score: 184.7
IPR036926	Thymidylate synthase/dCMP hydroxymethylase superfamily	SUPERFAMILY	55831	Thymidylate synthase/dCMP hydroxymethylase	coord: 242..562
IPR023451	Thymidylate synthase/dCMP hydroxymethylase domain	PFAM	PF00303	Thymidylat_synt	coord: 248..562 e-value: 6.0E-110 score: 366.6
IPR023451	Thymidylate synthase/dCMP hydroxymethylase domain	CDD	cd00351	TS_Pyrimidine_HMase	coord: 248..515 e-value: 2.78914E-110 score: 326.155
IPR001796	Dihydrofolate reductase domain	PFAM	PF00186	DHFR_1	coord: 25..201 e-value: 2.3E-38 score: 131.5
IPR001796	Dihydrofolate reductase domain	PROSITE	PS51330	DHFR_2	coord: 24..201 score: 53.365257
IPR001796	Dihydrofolate reductase domain	CDD	cd00209	DHFR	coord: 28..200 e-value: 2.57363E-61 score: 197.746
IPR012262	Bifunctional dihydrofolate reductase/thymidylate synthase	PIRSF	PIRSF000389	DHFR-TS	coord: 439..562 e-value: 1.5E-63 score: 213.0 coord: 9..434 e-value: 2.4E-178 score: 591.8
None	No IPR available	PIRSR	PIRSR000389-1	PIRSR000389-1	coord: 14..426 e-value: 6.7E-189 score: 626.6
None	No IPR available	PANTHER	PTHR11548:SF14	BIFUNCTIONAL DIHYDROFOLATE REDUCTASE-THYMIDYLATE SYNTHASE	coord: 440..562
None	No IPR available	PANTHER	PTHR11548:SF14	BIFUNCTIONAL DIHYDROFOLATE REDUCTASE-THYMIDYLATE SYNTHASE	coord: 3..427
IPR045097	Thymidylate synthase/dCMP hydroxymethylase	PANTHER	PTHR11548	THYMIDYLATE SYNTHASE 1	coord: 3..427
IPR045097	Thymidylate synthase/dCMP hydroxymethylase	PANTHER	PTHR11548	THYMIDYLATE SYNTHASE 1	coord: 440..562
IPR017925	Dihydrofolate reductase conserved site	PROSITE	PS00075	DHFR_1	coord: 36..58

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS020549.1	MS020549.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006231	dTMP biosynthetic process
biological_process	GO:0006730	one-carbon metabolic process
biological_process	GO:0046654	tetrahydrofolate biosynthetic process
molecular_function	GO:0004146	dihydrofolate reductase activity
molecular_function	GO:0004799	thymidylate synthase activity
molecular_function	GO:0016741	transferase activity, transferring one-carbon groups