MS020541 (gene) Bitter gourd (TR) v1

BLAST of MS020541 vs. NCBI nr
Match: XP_022146185.1 (adoMet-dependent rRNA methyltransferase spb1 [Momordica charantia])

HSP 1 Score: 1546.2 bits (4002), Expect = 0.0e+00
Identity = 825/829 (99.52%), Postives = 827/829 (99.76%), Query Frame = 0

Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSAHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSAHAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSAHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMNEKGCPAFDLVLHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMNEKGCPAFDLVLHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMNEKGCPAFDLVLHDGS 120

Query: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPRGTFVTKVFRSQDYSSVLYCLKQLFEKVE 180
           PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPRGTFVTKVFRSQDYSSVLYCLKQLFEKVE
Sbjct: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPRGTFVTKVFRSQDYSSVLYCLKQLFEKVE 180

Query: 181 VDKPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH 240
           VDKPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH
Sbjct: 181 VDKPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH 240

Query: 241 RDGYEDGDMTLRKVSSASNFIWSDSPLEILGSVTSITFDDSACLPIKDHDLTTEEVKTLC 300
           RDGYEDGDMTLRKVSSASNFIWSDSPLEILGSVTSITFDDSACLPIKDHDLTTEEVKTLC
Sbjct: 241 RDGYEDGDMTLRKVSSASNFIWSDSPLEILGSVTSITFDDSACLPIKDHDLTTEEVKTLC 300

Query: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATSTSVKDAENEVKQDDDDKILNEMEQLTF 360
           DDLRVLGKQDFKHLLKWRLHIRKALSP+QKATSTSVKDAENEVKQDDDDKILNEMEQLTF
Sbjct: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPSQKATSTSVKDAENEVKQDDDDKILNEMEQLTF 360

Query: 361 NMERKKRREKKRLAKRIAKDKARKATGMQRDVMDDSYGDPDLFSLSSIKDKKGLRGIDST 420
           NMERKKRREKKRLAKRIAKDKARKATGMQRDVMDDSYGDPDLFSLSSIKDKKGLRGIDST
Sbjct: 361 NMERKKRREKKRLAKRIAKDKARKATGMQRDVMDDSYGDPDLFSLSSIKDKKGLRGIDST 420

Query: 421 EYDDNVEVGEYENHVAKVENDGSSASDIDSDEERRRYDERMETLLDQAYESFVARKEGST 480
           EYDDNVEVGEYENHVAKVENDGSSASDIDSDEERRRYDERME+LLDQAYESFVARKEGST
Sbjct: 421 EYDDNVEVGEYENHVAKVENDGSSASDIDSDEERRRYDERMESLLDQAYESFVARKEGST 480

Query: 481 KQRKRAKKAYSDDAEVLEEDENGGDGIQSDYDSDENLVDEDKNPLMVSLDDGEAPTQEEI 540
           KQRKRAKKAYSDDAEVLEEDENGGDGIQSDYDSDENLVDEDKNPLMVSLDDGEAPTQEEI
Sbjct: 481 KQRKRAKKAYSDDAEVLEEDENGGDGIQSDYDSDENLVDEDKNPLMVSLDDGEAPTQEEI 540

Query: 541 ANKWFSQDIFAEAAQEGDMEMLDSEDDMQVDEPKERTSVSKEAKSNISKNAREKSNISKN 600
           ANKWFSQDIFAEAAQEGDMEMLDSEDDMQVDEPKE T VSKEAKSNISKNAREKSNISKN
Sbjct: 541 ANKWFSQDIFAEAAQEGDMEMLDSEDDMQVDEPKEITCVSKEAKSNISKNAREKSNISKN 600

Query: 601 ARETDKADDGFEVVPAPATDSSDDSSSSEDEDPETKAEILACAKKMLRKKQREQILDDSY 660
           ARETDKADDGFEVVPAPATDSSDDSSSSEDEDPETKAEILACAKKMLRKKQREQILDDSY
Sbjct: 601 ARETDKADDGFEVVPAPATDSSDDSSSSEDEDPETKAEILACAKKMLRKKQREQILDDSY 660

Query: 661 NKYMFDDAGLPNWFLDEERRHRIPIKPVTKEEIAAMRAQFKEINARPAKKVAEAKARKKR 720
           NKYMFDDAGLPNWFLDEERRHRIPIKPVTKEEIAAMRAQFKEINARPAKKVAEAKARKKR
Sbjct: 661 NKYMFDDAGLPNWFLDEERRHRIPIKPVTKEEIAAMRAQFKEINARPAKKVAEAKARKKR 720

Query: 721 VAMKKLEKVRKKANAISDQADINDRSKRKMIDQLYKKAVPQRPKKEFVVAKKGVQVKVGK 780
           VAMKKLEKVRKKANAISDQADINDRSKRKMIDQLYKKAVPQRPKKEFVVAKKGVQVKVGK
Sbjct: 721 VAMKKLEKVRKKANAISDQADINDRSKRKMIDQLYKKAVPQRPKKEFVVAKKGVQVKVGK 780

Query: 781 GKVRVDRRMKKDARKHGMSKQGKGSKKGKNLKAQRGKGGSAKASSAKKG 830
           GKVRVDRRMKKDARKHGMSKQGKGSKKGKNLKAQRGKGGSAKASSAKKG
Sbjct: 781 GKVRVDRRMKKDARKHGMSKQGKGSKKGKNLKAQRGKGGSAKASSAKKG 829

BLAST of MS020541 vs. NCBI nr
Match: KAG7010927.1 (spb1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1362.8 bits (3526), Expect = 0.0e+00
Identity = 734/836 (87.80%), Postives = 778/836 (93.06%), Query Frame = 0

Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSAHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRS+HAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMNEKGCPAFDLVLHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGA+AIEQDITKPECKARLKKIMNEKGC AFDL+LHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGS 120

Query: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPRGTFVTKVFRSQDYSSVLYCLKQLFEKVE 180
           PNVGGAWAQEAMSQNSLVIDSVKLATQLLAP+G FVTKVFRSQDYSSVLYCLKQLFEKVE
Sbjct: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVE 180

Query: 181 VDKPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH 240
           VDKPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH
Sbjct: 181 VDKPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH 240

Query: 241 RDGYEDGDMTLRKVSSASNFIWSDSPLEILGSVTSITFDDSACLPIKDHDLTTEE----- 300
           RDGYEDGD+TLRKVSSASN+IWSDSPLEILG+VT ITFDD ACL IK+HDLTTEE     
Sbjct: 241 RDGYEDGDLTLRKVSSASNYIWSDSPLEILGTVTCITFDDPACLLIKNHDLTTEEVPVII 300

Query: 301 -VKTLCDDLRVLGKQDFKHLLKWRLHIRKALSPNQKATSTSVKDAENEVKQDDDDKILNE 360
            VK LCDDLRVLGKQDFKHLLKWRLHIRKALSPNQKAT TSVKDAENEVKQ++DDK+LNE
Sbjct: 301 WVKALCDDLRVLGKQDFKHLLKWRLHIRKALSPNQKATPTSVKDAENEVKQNEDDKLLNE 360

Query: 361 MEQLTFNMERKKRREKKRLAKRIAKDKARKATGMQRDVMDDSYGDPDLFSLSSIKDKKGL 420
           ME+LT+ +ERKK+REKK LAKR AKDKARKA GMQ DVM++ Y D +LFSLS+IK K  L
Sbjct: 361 MEELTYAIERKKKREKKLLAKRRAKDKARKAMGMQLDVMEEGYIDHELFSLSNIKGKNDL 420

Query: 421 RGIDSTEYD-DNVEVGEYENHVAKVENDGSSASDIDSDEERRRYDERMETLLDQAYESFV 480
           R IDST+YD DN E+GE+EN V   E+ GSSASDIDSDEERRRYDE ME LLDQAYESFV
Sbjct: 421 RVIDSTDYDVDNGELGEHENDVTNEEDHGSSASDIDSDEERRRYDEHMEELLDQAYESFV 480

Query: 481 ARKEGSTKQRKRAKKAYSDDAEVLEEDENGGDGIQSDYDSDENLVDEDKNPLMVSLDDGE 540
           ARKEGS KQRKRAKKAYSDDAE+LEEDENGGDG QSDYDSDENLVDED+NPLMVSLDDG 
Sbjct: 481 ARKEGSAKQRKRAKKAYSDDAELLEEDENGGDGFQSDYDSDENLVDEDRNPLMVSLDDGG 540

Query: 541 APTQEEIANKWFSQDIFAEAAQEGDMEMLDSEDDMQVDEPKERTSVSKEAKSNISKNARE 600
            PTQEEIANKWF QDIFAEAA++GD++ LDSEDDMQVD PKE+T+V+K AKSNISKNARE
Sbjct: 541 EPTQEEIANKWFGQDIFAEAAEDGDLKELDSEDDMQVDGPKEKTAVTKVAKSNISKNARE 600

Query: 601 KSNISKNARETDKADDGFEVVPAPATDSSDDSSS--SEDEDPETKAEILACAKKMLRKKQ 660
           KS IS NA+++ KADDGFEVVPAPATDSSDDSSS  SEDE+P+TKAEILACAKKM+RKKQ
Sbjct: 601 KSKISTNAKDSIKADDGFEVVPAPATDSSDDSSSEESEDEEPDTKAEILACAKKMVRKKQ 660

Query: 661 REQILDDSYNKYMFDDAGLPNWFLDEERRHRIPIKPVTKEEIAAMRAQFKEINARPAKKV 720
           REQILDDSYNKYMFDD GLP WFLDEE+RHR PIKP+TKEE+AAMRAQFKEI+ARPAKKV
Sbjct: 661 REQILDDSYNKYMFDDTGLPKWFLDEEKRHRQPIKPITKEEVAAMRAQFKEIDARPAKKV 720

Query: 721 AEAKARKKRVAMKKLEKVRKKANAISDQADINDRSKRKMIDQLYKKAVPQRPKKEFVVAK 780
           AEAKARKKRVAMKKLEKVRKKAN ISDQADI+DRSK KMIDQLYKKAVPQRPKKEFVVAK
Sbjct: 721 AEAKARKKRVAMKKLEKVRKKANIISDQADISDRSKSKMIDQLYKKAVPQRPKKEFVVAK 780

Query: 781 KGVQVKVGKGKVRVDRRMKKDARKHGMSKQGKGSKKGKNLKAQRGKGGSAKASSAK 828
           KGVQV+VGKGK  VDRRMKKDARKHGMSKQGKGSKKGKN KA RGKGGSAKAS  K
Sbjct: 781 KGVQVRVGKGKTLVDRRMKKDARKHGMSKQGKGSKKGKNSKAPRGKGGSAKASGKK 836

BLAST of MS020541 vs. NCBI nr
Match: XP_022943552.1 (putative rRNA methyltransferase [Cucurbita moschata])

HSP 1 Score: 1361.3 bits (3522), Expect = 0.0e+00
Identity = 730/830 (87.95%), Postives = 776/830 (93.49%), Query Frame = 0

Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSAHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRS+HAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMNEKGCPAFDLVLHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGA+AIEQDITKPECKARLKKIMNEKGC AFDL+LHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGS 120

Query: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPRGTFVTKVFRSQDYSSVLYCLKQLFEKVE 180
           PNVGGAWAQEAMSQNSLVIDSVKLATQLLAP+G FVTKVFRSQDYSSVLYCLKQLFEKVE
Sbjct: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVE 180

Query: 181 VDKPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH 240
           VDKPAASRSASAEIYVLGLRY APAKIDPRLLDVKHLFQGSVE QRKVVDVLRGTKQKRH
Sbjct: 181 VDKPAASRSASAEIYVLGLRYTAPAKIDPRLLDVKHLFQGSVESQRKVVDVLRGTKQKRH 240

Query: 241 RDGYEDGDMTLRKVSSASNFIWSDSPLEILGSVTSITFDDSACLPIKDHDLTTEEVKTLC 300
           RDGYEDGD+TLRKVSSASN+IWSDSPLEILG+VT ITFDD ACL IK+HDLTTEEVK LC
Sbjct: 241 RDGYEDGDLTLRKVSSASNYIWSDSPLEILGTVTCITFDDPACLLIKNHDLTTEEVKALC 300

Query: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATSTSVKDAENEVKQDDDDKILNEMEQLTF 360
           DDLRVLGKQDFKHLLKWRLHIRKALSPNQKAT TSVKDAENEVKQ++DDK+LNEME+LT+
Sbjct: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATLTSVKDAENEVKQNEDDKLLNEMEELTY 360

Query: 361 NMERKKRREKKRLAKRIAKDKARKATGMQRDVMDDSYGDPDLFSLSSIKDKKGLRGIDST 420
            +ERKK+REKK LAKR AKDKARKA GMQ DVM++ Y D +LFSLS+IK K  LR +DST
Sbjct: 361 AIERKKKREKKLLAKRRAKDKARKAMGMQLDVMEEGYIDHELFSLSNIKGKNDLRVVDST 420

Query: 421 EYD-DNVEVGEYENHVAKVENDGSSASDIDSDEERRRYDERMETLLDQAYESFVARKEGS 480
           +YD DN E+GE+EN V   E+ GSSASDIDSDEERRRYDE ME LLDQAYESFVARKEGS
Sbjct: 421 DYDVDNGELGEHENDVTNEEDHGSSASDIDSDEERRRYDEHMEELLDQAYESFVARKEGS 480

Query: 481 TKQRKRAKKAYSDDAEVLEEDENGGDGIQSDYDSDENLVDEDKNPLMVSLDDGEAPTQEE 540
            KQRKRAKKAYSDDAE+LEEDENGGDG QSDYDSDENLVDED+NPLMVSLDDG  PTQEE
Sbjct: 481 AKQRKRAKKAYSDDAELLEEDENGGDGFQSDYDSDENLVDEDRNPLMVSLDDGGEPTQEE 540

Query: 541 IANKWFSQDIFAEAAQEGDMEMLDSEDDMQVDEPKERTSVSKEAKSNISKNAREKSNISK 600
           IANKWF QDIFAEAA++GD++ LDSEDDMQVD PKE+T+V+K AKSNISKNAREKS IS 
Sbjct: 541 IANKWFGQDIFAEAAEDGDLKELDSEDDMQVDGPKEKTAVTKVAKSNISKNAREKSKIST 600

Query: 601 NARETDKADDGFEVVPAPATDSSDDSSS--SEDEDPETKAEILACAKKMLRKKQREQILD 660
           NA+++ KADDGFEVVPAPATDSS+DSSS  SEDE+P+TKAEILACAKKM+RKKQREQILD
Sbjct: 601 NAKDSIKADDGFEVVPAPATDSSNDSSSEESEDEEPDTKAEILACAKKMVRKKQREQILD 660

Query: 661 DSYNKYMFDDAGLPNWFLDEERRHRIPIKPVTKEEIAAMRAQFKEINARPAKKVAEAKAR 720
           DSYNKYMFDD GLP WFLDEE+RHR PIKP+TKEE+AAMRAQFKEI+ARPAKKVAEAKAR
Sbjct: 661 DSYNKYMFDDTGLPKWFLDEEKRHRQPIKPITKEEVAAMRAQFKEIDARPAKKVAEAKAR 720

Query: 721 KKRVAMKKLEKVRKKANAISDQADINDRSKRKMIDQLYKKAVPQRPKKEFVVAKKGVQVK 780
           KKRVAMKKLEKVRKKAN ISDQADI+DRSK KMIDQLYKKAVPQRPKKEFVVAKKGVQV+
Sbjct: 721 KKRVAMKKLEKVRKKANIISDQADISDRSKSKMIDQLYKKAVPQRPKKEFVVAKKGVQVR 780

Query: 781 VGKGKVRVDRRMKKDARKHGMSKQGKGSKKGKNLKAQRGKGGSAKASSAK 828
           VGKGK  VDRRMKKDARKHGMSKQGKGSKKGKN KA RGKGGSAKAS  K
Sbjct: 781 VGKGKTLVDRRMKKDARKHGMSKQGKGSKKGKNSKAPRGKGGSAKASGKK 830

BLAST of MS020541 vs. NCBI nr
Match: KAG6571106.1 (hypothetical protein SDJN03_30021, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1356.7 bits (3510), Expect = 0.0e+00
Identity = 727/830 (87.59%), Postives = 775/830 (93.37%), Query Frame = 0

Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSAHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRS+HAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMNEKGCPAFDLVLHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGA+AIEQDITKPECKARLKKIMNEKGC AFDL+LHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGS 120

Query: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPRGTFVTKVFRSQDYSSVLYCLKQLFEKVE 180
           PNVGGAWAQEAMSQNSLVIDSVKLATQLLAP+G FVTKVFRSQDYSSVLYCLKQLFEKVE
Sbjct: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVE 180

Query: 181 VDKPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH 240
           VDKPAASRSASAEIYVLGLRY+APAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH
Sbjct: 181 VDKPAASRSASAEIYVLGLRYRAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH 240

Query: 241 RDGYEDGDMTLRKVSSASNFIWSDSPLEILGSVTSITFDDSACLPIKDHDLTTEEVKTLC 300
           RDGYEDGD+TLRKVSSASN+IWSDSPLEILG+VT ITFDD ACL IK+HDLTTEEVK LC
Sbjct: 241 RDGYEDGDLTLRKVSSASNYIWSDSPLEILGTVTCITFDDPACLLIKNHDLTTEEVKALC 300

Query: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATSTSVKDAENEVKQDDDDKILNEMEQLTF 360
           DDLRVLGKQDFKHLLKWRLHIRKALSPNQKAT TSVKDAENEVKQ++DDK+LNEME+LT+
Sbjct: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATLTSVKDAENEVKQNEDDKLLNEMEELTY 360

Query: 361 NMERKKRREKKRLAKRIAKDKARKATGMQRDVMDDSYGDPDLFSLSSIKDKKGLRGIDST 420
            +ERKK+REKK LAKR AKDKARKA GMQ DVM++ Y D +LFSLS+IK K  LR +DST
Sbjct: 361 AIERKKKREKKLLAKRRAKDKARKAMGMQLDVMEEGYIDHELFSLSNIKGKNDLRVVDST 420

Query: 421 EY-DDNVEVGEYENHVAKVENDGSSASDIDSDEERRRYDERMETLLDQAYESFVARKEGS 480
           +Y  DN E+GE+EN V   E+ GSSASDIDSDEERRRYDE ME LLDQAYESFVARKEGS
Sbjct: 421 DYVVDNGELGEHENDVTNEEDHGSSASDIDSDEERRRYDEHMEELLDQAYESFVARKEGS 480

Query: 481 TKQRKRAKKAYSDDAEVLEEDENGGDGIQSDYDSDENLVDEDKNPLMVSLDDGEAPTQEE 540
            KQRKRAKKAYSDDAE+LEEDENGGDG QSDYDSDENLVDED+NPLMVSLDDG  PTQEE
Sbjct: 481 AKQRKRAKKAYSDDAELLEEDENGGDGFQSDYDSDENLVDEDRNPLMVSLDDGGEPTQEE 540

Query: 541 IANKWFSQDIFAEAAQEGDMEMLDSEDDMQVDEPKERTSVSKEAKSNISKNAREKSNISK 600
           IANKWF QDIFA+AA++GD++ LDSEDDMQVD PKE+T+V+K AKSNISKNAREKS IS 
Sbjct: 541 IANKWFGQDIFAKAAEDGDLKELDSEDDMQVDGPKEKTAVTKVAKSNISKNAREKSKIST 600

Query: 601 NARETDKADDGFEVVPAPATDSSDDSSS--SEDEDPETKAEILACAKKMLRKKQREQILD 660
           NA+++ KADDGFEVVP PATDSS+DSSS  SEDE+P+TKAEILACAKKM+RKKQREQILD
Sbjct: 601 NAKDSIKADDGFEVVPPPATDSSNDSSSEESEDEEPDTKAEILACAKKMVRKKQREQILD 660

Query: 661 DSYNKYMFDDAGLPNWFLDEERRHRIPIKPVTKEEIAAMRAQFKEINARPAKKVAEAKAR 720
           DSYNKYMFDD GLP WFLDEE+RH  PIKP+TKEE+AAMRAQFKEI+ARPAKKVAEAKAR
Sbjct: 661 DSYNKYMFDDTGLPKWFLDEEKRHHQPIKPITKEEVAAMRAQFKEIDARPAKKVAEAKAR 720

Query: 721 KKRVAMKKLEKVRKKANAISDQADINDRSKRKMIDQLYKKAVPQRPKKEFVVAKKGVQVK 780
           KKRVAMKKLEKVRKKAN ISDQADI+DRSK KMIDQLYKKAVPQRPKKEFVVAKKGVQV+
Sbjct: 721 KKRVAMKKLEKVRKKANIISDQADISDRSKSKMIDQLYKKAVPQRPKKEFVVAKKGVQVR 780

Query: 781 VGKGKVRVDRRMKKDARKHGMSKQGKGSKKGKNLKAQRGKGGSAKASSAK 828
           VGKGK  VDRRMKKDARKHGMSKQGKGSKKGKN KA RGKGGSAKAS  K
Sbjct: 781 VGKGKTLVDRRMKKDARKHGMSKQGKGSKKGKNSKAPRGKGGSAKASGKK 830

BLAST of MS020541 vs. NCBI nr
Match: KAG6605073.1 (hypothetical protein SDJN03_02390, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1355.9 bits (3508), Expect = 0.0e+00
Identity = 734/835 (87.90%), Postives = 774/835 (92.69%), Query Frame = 0

Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSAHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRS+HAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMNEKGCPAFDLVLHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGA+AIEQDIT+PECKARLKKIMNEKGC AFDLVLHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGALAIEQDITRPECKARLKKIMNEKGCAAFDLVLHDGS 120

Query: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPRGTFVTKVFRSQDYSSVLYCLKQLFEKVE 180
           PNVGGAWAQEAMSQNSLVIDSVKLAT LLAP+GTFVTKVFRSQDYSSVLYCLKQLFEKVE
Sbjct: 121 PNVGGAWAQEAMSQNSLVIDSVKLATHLLAPKGTFVTKVFRSQDYSSVLYCLKQLFEKVE 180

Query: 181 VDKPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH 240
           VDKPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH
Sbjct: 181 VDKPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH 240

Query: 241 RDGYEDGDMTLRKVSSASNFIWSDSPLEILGSVTSITFDDSACLPIKDHDLTTEEVKTLC 300
           RDGYEDGDMTLRK SSASNFIWSDSPLEILG+VT+ITF+D ACLPIKDH+LTTEEVKTLC
Sbjct: 241 RDGYEDGDMTLRKESSASNFIWSDSPLEILGTVTTITFNDPACLPIKDHELTTEEVKTLC 300

Query: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATSTSVKDAENEVKQDDDDKILNEMEQLTF 360
           DDL VLGKQDFKHLLKWRLHIRKALSP QK TSTSV DAENEVKQD+DDK+LNEME+LT+
Sbjct: 301 DDLGVLGKQDFKHLLKWRLHIRKALSPKQKVTSTSVNDAENEVKQDEDDKLLNEMEELTY 360

Query: 361 NMERKKRREKKRLAKRIAKDKARKATGMQRDVMDDSYGDPDLFSLSSIKDKKGLRGIDST 420
            MERKK+REKK LAKR AKDKARKA GMQ DVM+D Y D +LFSLS+IK K  LR +DST
Sbjct: 361 AMERKKKREKKLLAKRKAKDKARKALGMQLDVMEDGYIDHELFSLSNIKGKNDLRVVDST 420

Query: 421 EY-DDNVEVGEYENHVAKVENDGSSASDIDSDEERRRYDERMETLLDQAYESFVARKEGS 480
           EY DDN E+GE EN V K EN GSS SDIDSDEERRRYD  ME LLDQAYESFVARKEGS
Sbjct: 421 EYDDDNDEMGEQENGVTKEENHGSSDSDIDSDEERRRYDAHMEELLDQAYESFVARKEGS 480

Query: 481 TKQRKRAKKAYSDDAEVLEEDENGGDGIQSDYDSDENLVDEDKNPLMVSLDDGEAPTQEE 540
           TKQRKRAKKAYSDDAE+L+EDENGGDG  SDYDSDENLVDEDKNPLMVSLDDG  PT+EE
Sbjct: 481 TKQRKRAKKAYSDDAELLKEDENGGDG-PSDYDSDENLVDEDKNPLMVSLDDGGVPTEEE 540

Query: 541 IANKWFSQDIFAEAAQEGDM-EMLDSEDDMQVDEPKERTSVSKEAKSNISKNAREKSNIS 600
           IANKWFSQDIFAEA ++GD+ E++DSEDDMQ+D PKE+ +VSKEAK NISK  REKS  S
Sbjct: 541 IANKWFSQDIFAEAVEDGDLKELVDSEDDMQIDRPKEKAAVSKEAKPNISKKEREKSKTS 600

Query: 601 KNARETDKADDGFEVVPAPATDSSDDSSS--SEDEDPETKAEILACAKKMLRKKQREQIL 660
            NARETDK DDGFEVVPAPATDSSDDSSS  SEDEDP+TKAEILACAKKMLRKKQREQIL
Sbjct: 601 TNARETDKEDDGFEVVPAPATDSSDDSSSDESEDEDPDTKAEILACAKKMLRKKQREQIL 660

Query: 661 DDSYNKYMFDDAGLPNWFLDEERRHRIPIKPVTKEEIAAMRAQFKEINARPAKKVAEAKA 720
           DD+YNKYMFDD GLP+WFLDEERRHR PIKP+TKEE+AAM+AQFKEI+ARPAKKVAEAKA
Sbjct: 661 DDAYNKYMFDDTGLPSWFLDEERRHRQPIKPITKEEVAAMKAQFKEIDARPAKKVAEAKA 720

Query: 721 RKKRVAMKKLEKVRKKANAISDQADINDRSKRKMIDQLYKKAVPQRPKKEFVVAKKGVQV 780
           RKKRVAMKKLEKVRKKAN+ISDQADI+DRSKRKMIDQLYKKAVP+RPKKEFVVAKKGVQV
Sbjct: 721 RKKRVAMKKLEKVRKKANSISDQADISDRSKRKMIDQLYKKAVPERPKKEFVVAKKGVQV 780

Query: 781 KVGKGKVRVDRRMKKDARKHGMSKQGKGSK----KGKNLKAQRGKGGSAKASSAK 828
           +VGKGKV VDRRMKKD RKHGMSKQGKGSK    KGKN KA RGKGG AKAS  K
Sbjct: 781 RVGKGKVLVDRRMKKDMRKHGMSKQGKGSKKGKGKGKNSKAPRGKGGPAKASGKK 834

BLAST of MS020541 vs. ExPASy Swiss-Prot
Match: O42832 (AdoMet-dependent rRNA methyltransferase spb1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=spb1 PE=1 SV=2)

HSP 1 Score: 427.9 bits (1099), Expect = 2.5e-118
Identity = 315/833 (37.82%), Postives = 476/833 (57.14%), Query Frame = 0

Query: 3   KVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSAHAVLDLCAAPGGWMQVAVE 62
           K   K RLDK+Y+LAKE GYRSRA++KL QL+ KY+FL  A  ++DLCAAPGGW+QVA +
Sbjct: 6   KKTAKGRLDKWYKLAKEQGYRSRAAFKLVQLNQKYSFLEKAKVIIDLCAAPGGWLQVASK 65

Query: 63  RVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMNEKGCPAFDLVLHDGSPN 122
               GSL+VGVDL PI P+       +DIT  +C+++L+  +        D+VLHDG+PN
Sbjct: 66  TCKPGSLIVGVDLAPIKPIPNCHTFVEDITSDKCRSQLRGYLKTWKA---DVVLHDGAPN 125

Query: 123 VGGAWAQEAMSQNSLVIDSVKLATQLLAPRGTFVTKVFRSQDYSSVLYCLKQLFEKVEVD 182
           VG AW Q+A  Q  LV+ S+KLA + L   GTFVTKVFRS+DY+++L+  KQLF KVE  
Sbjct: 126 VGSAWLQDAYGQAQLVLMSMKLACEFLVAGGTFVTKVFRSRDYNNLLWVFKQLFNKVEAT 185

Query: 183 KPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEPQRKV-VDVLRGTKQKRHR 242
           KP +SR+ SAEI+V+   YKAP K+DPR  D + +F+   EP   V   V    K+KR R
Sbjct: 186 KPPSSRNVSAEIFVVCRGYKAPKKLDPRFTDPRTVFEEVQEPVTNVDAKVFHPEKRKRSR 245

Query: 243 DGYEDGDMTLRKVSSASNFIWSDSPLEILGSVTSITF--DDSACLPIKDHDLTTEEVKTL 302
           +GY D D TL K   AS F+ ++ P++ILG+   I F  DD  C  + + D+TTEE+   
Sbjct: 246 EGYADDDYTLHKTVLASEFVTANDPIQILGTSAEIVFPKDDEECQRLYNLDVTTEEILLC 305

Query: 303 CDDLRVLGKQDFKHLLKWRLHIR------KALSPNQKATSTSVKDAENEVKQDDDDKILN 362
           C DL+VLGK++F+ +L+WRL IR      K +   QK     + + + E + D + + L+
Sbjct: 306 CSDLQVLGKKEFRDILRWRLKIRDEMGIGKKVEDEQKTVVEEIPEMDEEERLDQELQDLS 365

Query: 363 EMEQLTFNMERKKRREKKRLAKRIAKDKARKATGMQRDVMDDSYGDPDLFSLSSIKDKKG 422
           E E++    ER+K  ++K+  + I + +      M   +  ++ G+  LF L++  +K G
Sbjct: 366 EAERVKLKRERRKANQRKQ--REIVRMQMGMLAPMDIGLEHEAMGEDSLFGLAT-AEKHG 425

Query: 423 LRGIDSTEYDDNVEVGEYENHVAKVENDGSSASDIDSDEERRRYDERMETLLDQAYESFV 482
           L+ ++    +  + V E  +     +N+     + DSD+ER    +R+E  LD  Y  + 
Sbjct: 426 LKELE----NGTLPVTESVDEEVSTDNE----VEYDSDDER----DRLEADLDSMYSDYT 485

Query: 483 ARKEGSTKQRKRAKKAYSDDAEVLEEDENGGD-GIQSDYD--SDENLVDEDKNPLMVSLD 542
            RK   +  + R KKA  D   + +E+ NG D G +SD    ++ N    DK+ L  SL 
Sbjct: 486 KRK-AESDVKYRVKKARGD---LDDEEWNGIDNGTESDDSQIAETNFATPDKDRLTTSLL 545

Query: 543 DGEAPTQEEIANK---WFSQDIFAEAAQEGDMEMLDSEDDMQVDEPKERTSVSKEA-KSN 602
           D +  T++ ++ K   +F QDIF            D  +D   D   E  S+++ A K  
Sbjct: 546 D-KGSTKDGLSRKARMFFDQDIF------------DGIEDADAD--VEIMSMNRAAIKKR 605

Query: 603 ISKNAREKSNISKNARETDKADDGFEVVPAPATDSSDDSSSSEDEDPETKAEILACAKKM 662
            ++ A + ++      +++ ++D  EVVP  +    DD  +S+ ++ E   EI+      
Sbjct: 606 EAELASQNNDDGSKGDQSEDSNDHIEVVPVASAHDEDDDWNSDSDNDENNVEIVTAEAMT 665

Query: 663 L------RKKQREQILDDSYNKYMFDD-AGLPNWFLDEERRHRIPIKPVTKEEIAAMRAQ 722
           L      R+K +  ++D+ YN++ F    GLP+WFLDEE     P KP+TKE + A+R +
Sbjct: 666 LAQDIASRRKSKADLIDEGYNRWSFQSKEGLPDWFLDEETTVNKPNKPITKEAVLALREK 725

Query: 723 FKEINARPAKKVAEAKARKKRVAMKKLEKVRKKANAISDQADINDRSKRKMIDQLYKKAV 782
            K +NARP KKV EA+ RKK   +K+L++V KKA  IS+  D+ +  K K I +L  +A 
Sbjct: 726 MKALNARPIKKVLEAQGRKKMRTIKRLQRVAKKAEGISESGDMTESEKAKEISRLVSRAT 785

Query: 783 PQRPKKE--FVVAK---KGV--QVKVGKGKVR-VDRRMKKDAR-KHGMSKQGK 804
             +PK +   VVAK   KG+  + K  KGK + VD RMKKD R +  ++K+G+
Sbjct: 786 KSKPKAKPTLVVAKGPNKGLKSRPKGVKGKYKMVDSRMKKDLRAQKRLAKKGR 801

BLAST of MS020541 vs. ExPASy Swiss-Prot
Match: Q54NX0 (pre-rRNA 2'-O-ribose RNA methyltransferase OS=Dictyostelium discoideum OX=44689 GN=fsjC PE=3 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 8.2e-117
Identity = 323/862 (37.47%), Postives = 492/862 (57.08%), Query Frame = 0

Query: 3   KVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSAHAVLDLCAAPGGWMQVAVE 62
           K   K RLDK+Y +AKE GYRSRA++KL QL+ KYNFL +A A LDLCAAPGGWMQVA +
Sbjct: 6   KKLAKGRLDKFYYMAKEQGYRSRAAFKLIQLNKKYNFLGTAKACLDLCAAPGGWMQVASK 65

Query: 63  RVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMNEKGCPAFDLVLHDGSPN 122
            +PV SL+VGVDLVPI  VR  + + +DIT  +C+  +KK +        D+ LHDG+PN
Sbjct: 66  YMPVQSLIVGVDLVPIRQVRNCIGLTEDITTQKCRTEIKKALKTW---KVDVCLHDGAPN 125

Query: 123 VGGAWAQEAMSQNSLVIDSVKLATQLLAPRGTFVTKVFRSQDYSSVLYCLKQLFEKVEVD 182
           +G +W Q+A  Q  L + ++KLAT+ L   G FVTKVFR  DY+S+++   +LF+KVE  
Sbjct: 126 MGTSWVQDAYQQAELTLHALKLATEFLTTGGWFVTKVFRGSDYNSLIWVFNKLFKKVEST 185

Query: 183 KPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRHRD 242
           KP +SR+ASAEI+V+   +  P +IDP+LLD K +F+   E   K VDVL   K+K +R 
Sbjct: 186 KPPSSRNASAEIFVVCQGFLNPKRIDPKLLDPKFVFKEIQEV--KKVDVL-SEKKKVNRA 245

Query: 243 GYEDGDMTLRKVSSASNFIWSDSPLEILGSVTSITFDDSACLPIKDHDLTTEEVKTLCDD 302
           GYEDG   L K    S+F+ S+  L+ L +  +  FD++A +  + H+LTT E+K L  D
Sbjct: 246 GYEDGVTVLYKKGFISDFVNSNEHLQDLANFNAFEFDEAAKI-FEQHELTTPEIKELVKD 305

Query: 303 LRVLGKQDFKHLLKWR----LHIRKALSPNQKATSTSVKDAENEVKQDDDDKILNEMEQL 362
           L+VL K DF+ ++KW+     +  K  +P+++ T    ++ +    ++ ++ +  EM++ 
Sbjct: 306 LKVLNKNDFQKIIKWKKAMAAYKEKLDNPDEEETE-KPEEKKELTAEEMEENLQEEMKEY 365

Query: 363 TFNMERKKRREKKRL--AKRIAKDKARKATGMQRDVMDDSYGDPDLFSLSSIKDKKGLRG 422
              +E+KKR+EKKR    KR  + K      +  D ++++  D DL+S         ++G
Sbjct: 366 LALVEKKKRKEKKRQNELKRKHQRKIELTMHIPGDKIEET-TDGDLYS---------MKG 425

Query: 423 IDSTEYDDNVEVGEYENHVAKVENDGSSASDID---SDEERRRYDERMETLLDQAYESFV 482
            D  E+D+++     +    + ++D S   D D    D +    DE +E  LD+ Y+ + 
Sbjct: 426 KD--EFDEDIVADHSDISSDEFDSDDSDDDDDDDNNGDSKLIDDDEYLEQQLDEQYKLY- 485

Query: 483 ARKEGSTKQRKRAKKAYSDDAEVLEEDENGGDGIQSDYDSDENLVD--EDKNPLMVSLDD 542
                  +QR R K A  DD +V ++D+ G DG   D   DE  V+  E+ NPL+V  + 
Sbjct: 486 -------QQRIRKKAAKLDDVKV-KKDKIGQDGYNED---DEEFVEEQEESNPLLVG-NK 545

Query: 543 GEAPTQEEIANKWFSQDIF------------AEAAQEGDMEMLDSEDDMQVD-------E 602
            + P  + +++ +F  ++F            +E  Q+GD +  D E++  +D       +
Sbjct: 546 RKEPDAQAVSSLFFDNELFGGVEYRNPGDSESEPEQDGDDDQ-DDENNKPIDISKLKKQK 605

Query: 603 PKERTSVSKEAK-SNISKNAREKSNISKNARETDKADD-----------GFEVVPAPATD 662
           P+    ++K+ K +N ++  ++KS   KN    DK D            GFE VP     
Sbjct: 606 PQAAQPITKKQKTTNSAEFGKQKSKYQKNPTLDDKDDQDDDDDKGNSIKGFEEVP---VQ 665

Query: 663 SSDDSSSSEDEDPETKAEILACAKKMLRKKQREQILDDSYNKYMFDDAGLPNWFLDEERR 722
              +  S  DED + K +  A  + ++RKK R+ ++DDS+NKY F+D GLPNWF D+E R
Sbjct: 666 EEVEYESDSDEDIDDKIKTKALGEFLIRKKSRQDLIDDSFNKYAFNDTGLPNWFTDDENR 725

Query: 723 HRIPIKPVTKEEIAAMRAQFKEINARPAKKVAEAKARKKRVAMKKLEKVRKKANAISDQA 782
           H     P+TKE +  +R + KEI+ RP KK+AEAKARKK    KK+EK R KA++I D  
Sbjct: 726 HNKAQTPLTKEMVDEIRRKIKEIDDRPIKKIAEAKARKKYRLGKKMEKTRDKASSIVDNP 785

Query: 783 DINDRSKRKMIDQLYKKAVPQ--RPKKEFVVAKKGVQVKVGKGKVR-VDRRMKKD--ARK 818
           ++++R K K I++LY     +  +PKK  ++AKK      G GK + VD+RMKKD  A+K
Sbjct: 786 EMSNREKSKAIEKLYSGTDKKNMKPKKIIMIAKKSKTAGGGTGKYKIVDKRMKKDLRAQK 830

BLAST of MS020541 vs. ExPASy Swiss-Prot
Match: Q52C47 (AdoMet-dependent rRNA methyltransferase SPB1 OS=Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) OX=242507 GN=SPB1 PE=3 SV=2)

HSP 1 Score: 411.0 bits (1055), Expect = 3.2e-113
Identity = 315/867 (36.33%), Postives = 481/867 (55.48%), Query Frame = 0

Query: 3   KVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSAHAVLDLCAAPGGWMQVAVE 62
           K  GK RLDK+Y+LAKE GYR+RA++KL QL+ KY FL  +  +LDLCAAPG W QVA E
Sbjct: 5   KKHGKGRLDKWYKLAKEKGYRARAAFKLIQLNKKYGFLEKSKVLLDLCAAPGSWCQVAAE 64

Query: 63  RVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMNEKGCPAFDLVLHDGSPN 122
            +PV SL+VGVDL PI P+   +  + DIT  +C+A +++ +        D VLHDG+PN
Sbjct: 65  VMPVSSLIVGVDLAPIKPIPKVITFQSDITTEKCRATIRQHLKTWKA---DTVLHDGAPN 124

Query: 123 VGGAWAQEAMSQNSLVIDSVKLATQLLAPRGTFVTKVFRSQDYSSVLYCLKQLFEKVEVD 182
           VG AW Q++ +Q  L + ++KLAT+ L   GTFVTKVFRS+DY+S+L+   QLF+KVE  
Sbjct: 125 VGTAWVQDSFNQAELTLQAMKLATEFLVEGGTFVTKVFRSKDYNSMLWVFNQLFKKVEAT 184

Query: 183 KPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEP-QRKVVDVLRGTKQKRHR 242
           KP +SR+ SAEI+V+   +KAP +IDP+ LD + +F    +P       V +   +KR R
Sbjct: 185 KPPSSRNVSAEIFVVCRGFKAPKRIDPKFLDPRAVFAELADPTPNNEAKVYKPEIKKRKR 244

Query: 243 DGYEDGDMTLRKVSSASNFIWSDSPLEILGSVTSITFD-----DSACLPIKDHDLTTEEV 302
           DGYE+GD T  K   A  FI S  P+ ILGS   ++ +     D A   ++    TT+E+
Sbjct: 245 DGYEEGDYTQYKELPAYEFIQSTDPIAILGSTNRLSLEQSKNGDVALAVLEKLPETTDEI 304

Query: 303 KTLCDDLRVLGKQDFKHLLKWRLHIRKALS-PNQKA------TSTSVKDAENEVK---QD 362
           +T C DL+VLG+++FK LLKWRL +R+ L  P +K+       + +V  AE   K    D
Sbjct: 305 RTCCADLKVLGRKEFKLLLKWRLAVREKLGFPTKKSVKKEEEAAAAVAAAEEVAKIESMD 364

Query: 363 DDDKILNEMEQLTFNMERKKRREKKRLAKRIAKDKARKATGMQRDV-----MDDSYGDPD 422
           ++ +I +E+E+L      KK+RE+++  +R  KD  R    M   +          G+  
Sbjct: 365 EEMRIQHELEKLKERNSTKKKRERRKENERKQKDIVRMQMHMVAPMDIGVEQAGPEGEDA 424

Query: 423 LFSLSSIKDKKGLRGIDSTEYDDNVEVGEYENHVAKVENDGSSASDIDSDEERRRYDERM 482
           +F+L +++    +R +   +    V   E +   AK + D    S  ++D+E     +R+
Sbjct: 425 MFALRAVEKGDVMRRLAKGKM---VVASEAD---AKKDRDSGIGSSGETDDESDEELDRL 484

Query: 483 ETLLDQAYESFVARKEGSTK--QRKRAKKAYSDDAEVLEEDENGGDGIQSDYDSDENLVD 542
           ET LD  Y+ F  RK  S    + K+A++A + D +  EE E   D  ++D  SD++ ++
Sbjct: 485 ETELDDMYDQFRERKAASDAKYRAKKARQARNGDGD--EEWEGVSDNEKADEISDDSELE 544

Query: 543 ED-------------KNPLMVSLDDGEAPTQ--EEIANKWFSQDIFAE------------ 602
           E+             +  L+  LD   +      + A  +F+QDIF E            
Sbjct: 545 EESSGDSDDEDDTAPRKSLLTDLDTTPSDNSGLSKRARAFFNQDIFKELDGDMDEPMDEE 604

Query: 603 -----AAQEGDMEMLDSEDDMQVDEPKERTSVSKEAKSNISKNAREKSNISKNARETDKA 662
                A ++ D +M D+       + KE+T+  K AK   +K A +K+   K+    D++
Sbjct: 605 LRAALAGEDEDADMEDTVSKADSKKTKEKTADKKAAKK--AKKAAQKAQQVKDDDSDDES 664

Query: 663 DDGFEVVPAPATDSSDDSSSSEDEDPETK----------AEILACAKKMLR-KKQREQIL 722
           D GFEVV       S      EDED  TK          AE +  A ++   +K    ++
Sbjct: 665 DGGFEVV------KSGKEDDWEDEDKRTKDGRLDIDIITAEAMTLAHQLATGQKSSHDVI 724

Query: 723 DDSYNKYMFDD-AGLPNWFLDEERRHRIPIKPVTKEEIAAMRAQFKEINARPAKKVAEAK 782
           DD +NK+ F D  GLP WFLD+E +H  P KP+TK   AA++ + +  NARP KKV EAK
Sbjct: 725 DDGFNKHAFKDREGLPEWFLDDETKHDKPQKPITKAAAAAIKEKMRAFNARPIKKVREAK 784

Query: 783 ARKKRVAMKKLEKVRKKANAISDQADINDRSKRKMIDQLYKKAVPQRPKK--EFVVAK-- 795
            RKK  A ++LEK++KK++ + ++  + ++ K + I +L +KA  ++PK+  + VVAK  
Sbjct: 785 GRKKMKAAQRLEKLKKKSDLLVNEEGMTEKEKAESIAKLLRKATKKKPKQAVKVVVAKGA 844

BLAST of MS020541 vs. ExPASy Swiss-Prot
Match: Q4WVH3 (AdoMet-dependent rRNA methyltransferase spb1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=spb1 PE=3 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 1.2e-112
Identity = 311/831 (37.42%), Postives = 453/831 (54.51%), Query Frame = 0

Query: 3   KVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSAHAVLDLCAAPGGWMQVAVE 62
           K  GK RLDK+YRLAKE GYR+RA++KL QL+ KY FL  +  +LDLCAAPG W QVA E
Sbjct: 5   KKHGKGRLDKWYRLAKEKGYRARAAFKLIQLNKKYGFLEKSKVLLDLCAAPGSWCQVAAE 64

Query: 63  RVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMNEKGCPAFDLVLHDGSPN 122
            +P  S+++GVDL PI P+   +  + DIT  +C+A +++ +        D VLHDG+PN
Sbjct: 65  CMPTQSIIIGVDLAPIKPIPRVITFQSDITTEKCRATIRQHLKHWKA---DTVLHDGAPN 124

Query: 123 VGGAWAQEAMSQNSLVIDSVKLATQLLAPRGTFVTKVFRSQDYSSVLYCLKQLFEKVEVD 182
           VG AW Q+A SQ  LV+ S+KLAT+ L   GTFVTKVFRS+DY+ +L+  KQLF  VE  
Sbjct: 125 VGTAWVQDAFSQAELVLQSMKLATEFLVEGGTFVTKVFRSKDYNPLLWVFKQLFTSVEAT 184

Query: 183 KPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVE-PQRKVVDVLRGTKQKRHR 242
           KP +SR+ SAEI+V+   +KAP +IDP+ LD KH+F    +        V    K+KR R
Sbjct: 185 KPPSSRNVSAEIFVVCRGFKAPKRIDPKFLDPKHVFAELTDSTPNNEARVFNPEKKKRKR 244

Query: 243 DGYEDGDMTLRKVSSASNFIWSDSPLEILGSVTSITFD-----DSACLPIKDHDLTTEEV 302
           +GYE+GD T  K    + FI +  P+ ILG+   ++F+     D A   +   + TT+E+
Sbjct: 245 EGYEEGDYTQFKEIPVTEFINTTDPIAILGTYNKLSFEQSPGGDLALATLNRLEETTDEI 304

Query: 303 KTLCDDLRVLGKQDFKHLLKWRLHIR------------KALSPNQKATSTSVKDAENEVK 362
           +T C+DL++LGK++F+ LL+WRL +R            KA  P + A    V   + E+ 
Sbjct: 305 RTCCEDLKILGKKEFRSLLRWRLKVREKFGLVVKKGQAKADEPEEVA---EVAPMDEELA 364

Query: 363 QDDDDKILNEMEQLTFNMERKKRREKKRLAKRIAKDKARKATGMQRDVMD-DSYGDPDLF 422
             ++ + L E E      ER+K  EKKR  K I + +    T M   +      GD   F
Sbjct: 365 IQEELQRLQEKESAKRKKERRKENEKKR--KEIIRMQMHMTTPMDIGMEQLGPGGDDATF 424

Query: 423 SLSSIKDKKGLRGIDSTEYDDNVEVGEYENHVAKVENDGSSASDIDSDEERRRYDERMET 482
           SL  + ++ G R        D +  G+     +  E+D + +   +SD+E     +R+E 
Sbjct: 425 SLKRV-ERDGAR--------DVIASGKLAEIESDSEDDQTESDYDESDDE----GDRLER 484

Query: 483 LLDQAYESFVARKEGSTKQRKRAKKAYSDDAEVLEEDENGGDGIQSDYDSDENLVDEDKN 542
            LD  YE +  R+E     + RAKKA  D      E E       SD + DE   ++  +
Sbjct: 485 ELDSLYEQYQERRE-DRDSKVRAKKARKD-----YEAEEWDGFSDSDKEDDEESEEDGAS 544

Query: 543 PLMVSLDDGEAPTQEEIANKWFSQDIFAEAAQEGDMEMLDSEDDMQVDEPKERTSVSKEA 602
             +V      + T    A  +F QDIF       D+E  DS  +MQ D+           
Sbjct: 545 QAVVKPAPPNSGTLSSKAAMFFDQDIFQGLGDVDDVEDEDSAIEMQEDD----------- 604

Query: 603 KSNISKNAREKSNISKNARETDKADDGFEVVPAPATDSSDDSSSSEDEDPETK------- 662
                K+A++ S + K A +  K         A A +   DS   E +DP  K       
Sbjct: 605 -----KSAKKGSALEKKAPKEAKK-------KAQAPEDFSDSDPEEPDDPRKKNGQLDID 664

Query: 663 ---AEILACAKKMLR-KKQREQILDDSYNKYMFDDA-GLPNWFLDEERRHRIPIKPVTKE 722
              AE +A A++M   +K+ + I+DD +N+Y F D  GLP WFLD+E +H  P +P+TK 
Sbjct: 665 IITAEAMALAQQMATGEKKSQDIIDDGFNRYTFRDVDGLPEWFLDDENKHSKPQRPITKA 724

Query: 723 EIAAMRAQFKEINARPAKKVAEAKARKKRVAMKKLEKVRKKANAISDQADINDRSKRKMI 782
             AA++ + + INARP KKV EAK RKK  A ++LEK+RKK+  ++D   +++R K + I
Sbjct: 725 AAAAIKEKLRAINARPIKKVMEAKGRKKMKAAQRLEKLRKKSALLADDEALSERDKSQAI 784

Query: 783 DQLYKKAVPQRPKKE--FVVAK---KGV--QVKVGKGKVR-VDRRMKKDAR 795
            +L  KAV ++PK++   VVA+   +G+  + +  KGK + VD RMKKD R
Sbjct: 785 AKLMSKAVKKKPKQQVKLVVARGANRGISGRPRGVKGKYKIVDSRMKKDIR 785

BLAST of MS020541 vs. ExPASy Swiss-Prot
Match: Q5BH88 (AdoMet-dependent rRNA methyltransferase spb1 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=spb1 PE=3 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 2.7e-112
Identity = 314/829 (37.88%), Postives = 454/829 (54.76%), Query Frame = 0

Query: 3   KVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSAHAVLDLCAAPGGWMQVAVE 62
           K  GK RLDK+YRLAKE GYR+RA++KL QL+ KY FL  +  +LDLCAAPG W QVA E
Sbjct: 5   KKHGKGRLDKWYRLAKEKGYRARAAFKLVQLNKKYGFLEKSKVLLDLCAAPGSWCQVAAE 64

Query: 63  RVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMNEKGCPAFDLVLHDGSPN 122
            +P  SL+VGVDL PI P+   +  +QDIT  +C+A ++  +        D VLHDG+PN
Sbjct: 65  CMPAQSLIVGVDLAPIKPIPRVITFQQDITTEKCRATIRSHLKHWKA---DTVLHDGAPN 124

Query: 123 VGGAWAQEAMSQNSLVIDSVKLATQLLAPRGTFVTKVFRSQDYSSVLYCLKQLFEKVEVD 182
           VG AW Q+A SQ  LV++S+KLAT+ L   GTFVTKVFRS+DY+ +L+  KQLF  VE  
Sbjct: 125 VGTAWVQDAFSQAELVLESLKLATEFLVEGGTFVTKVFRSKDYNPLLWVFKQLFMSVEAT 184

Query: 183 KPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEP-QRKVVDVLRGTKQKRHR 242
           KP +SR+ SAEI+V+   YKAP +IDP+ LD KH+F    +P       V    K+KR R
Sbjct: 185 KPPSSRNVSAEIFVVCRGYKAPKRIDPKFLDSKHVFAELADPTPNNEAKVFNPEKKKRKR 244

Query: 243 DGYEDGDMTLRKVSSASNFIWSDSPLEILGSVTSITFD-----DSACLPIKDHDLTTEEV 302
           +GYE+GD T  K    + FI +  P+ ILGS   ++F      D A   +     TT+E+
Sbjct: 245 EGYEEGDWTQFKEIPVTEFINTTDPIAILGSCNKLSFQQQPGGDLALATLDRLPETTDEI 304

Query: 303 KTLCDDLRVLGKQDFKHLLKWRLHIRKALSPNQKATSTSVKDAEN--EVKQDDD-----D 362
           +  C+DL+VLGK++F++LL+WRL +R+      K       +AE   E+   DD     +
Sbjct: 305 RNCCEDLKVLGKKEFRNLLRWRLKVREKFGLVVKKGQAKDDEAEEVAEIAPMDDELAIQE 364

Query: 363 KILNEMEQLTFNMERKKRREKKRLAKRIAKDKARKATGMQRDVMDDSYGDPDLFSLSSIK 422
           ++L   E+ +   ++++R+E +R  K I + +    T M   +     G  D        
Sbjct: 365 ELLRLKEKESARSKKERRKENERKRKEIVRMQMHMTTPMDIGMEQLGPGGEDATFSLKRA 424

Query: 423 DKKGLRGIDSTEYDDNVEVGEYENHVAKVENDGSSASDIDSDEERRRYDERMETLLDQAY 482
           +K G     ++  +  V   E E   ++ E D       DSD+E     +R+E  LD  Y
Sbjct: 425 EKAGAAAAMASGKELAVIESESEESESETEYD-------DSDDE----GDRLERELDSLY 484

Query: 483 ESFVARKEGSTKQRKRAKKAYSDDAEVLEEDENGGDGIQSDYDSDENLVDEDKNPLMVSL 542
           E +  RKE     + RAKKA  D     E DE  G    SD +  ++  DE K  + V  
Sbjct: 485 EQYQERKE-DKDSKLRAKKARKD----YEADEWEGFS-DSDKEGSDDEEDEAKPNVPV-- 544

Query: 543 DDGEAPTQEEIANK---WFSQDIFAEAAQ-EGDMEMLDSEDDMQVDEPKERTSVSKEAKS 602
                PT   ++N    +F QDIF      E + +  +  ++M V   +E     +E + 
Sbjct: 545 ----VPTNGALSNNAALFFDQDIFQGLDDVEDEEDEAEGTNNMTVANEREEQQEEEEEEQ 604

Query: 603 NISKNAREKSNISKNARETDKADDGFEVVPAPATDSSDDSSSSEDEDPETK--------- 662
             S+ A EK    K  +ET  +         P+ DSSDD     DE P  K         
Sbjct: 605 PKSEAAPEK---KKKVKETANS--------KPSEDSSDDEYEDTDE-PRKKNGQLDIDII 664

Query: 663 -AEILACAKKMLR-KKQREQILDDSYNKYMFDDA-GLPNWFLDEERRHRIPIKPVTKEEI 722
            AE +A A++M   +K+ + + DD +N+Y F D  GLP WFLD+E +H  P +P+TK   
Sbjct: 665 TAEAMALAQQMATGEKKSQDVFDDGFNRYAFRDVDGLPEWFLDDENKHSKPNRPITKAAA 724

Query: 723 AAMRAQFKEINARPAKKVAEAKARKKRVAMKKLEKVRKKANAISDQADINDRSKRKMIDQ 782
           AA++ +++ INARP KKV EAK RKK  A +++EK+RKK+  ++D   +++R K + I +
Sbjct: 725 AAIQEKWRAINARPIKKVMEAKGRKKFKAAQRIEKLRKKSALLADDETMSERDKAQAIAR 784

Query: 783 LYKKAVPQRPKK--EFVVAKKGVQVKVG-----KGKVR-VDRRMKKDAR 795
           +  +A  ++PK+  + VVAK G +   G     KGK + VD RMKKD R
Sbjct: 785 MMSRAAKKKPKQNVKLVVAKGGNRGISGRPKGVKGKYKIVDARMKKDVR 795

BLAST of MS020541 vs. ExPASy TrEMBL
Match: A0A6J1CWK4 (Putative rRNA methyltransferase OS=Momordica charantia OX=3673 GN=LOC111015464 PE=3 SV=1)

HSP 1 Score: 1546.2 bits (4002), Expect = 0.0e+00
Identity = 825/829 (99.52%), Postives = 827/829 (99.76%), Query Frame = 0

Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSAHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSAHAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSAHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMNEKGCPAFDLVLHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMNEKGCPAFDLVLHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMNEKGCPAFDLVLHDGS 120

Query: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPRGTFVTKVFRSQDYSSVLYCLKQLFEKVE 180
           PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPRGTFVTKVFRSQDYSSVLYCLKQLFEKVE
Sbjct: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPRGTFVTKVFRSQDYSSVLYCLKQLFEKVE 180

Query: 181 VDKPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH 240
           VDKPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH
Sbjct: 181 VDKPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH 240

Query: 241 RDGYEDGDMTLRKVSSASNFIWSDSPLEILGSVTSITFDDSACLPIKDHDLTTEEVKTLC 300
           RDGYEDGDMTLRKVSSASNFIWSDSPLEILGSVTSITFDDSACLPIKDHDLTTEEVKTLC
Sbjct: 241 RDGYEDGDMTLRKVSSASNFIWSDSPLEILGSVTSITFDDSACLPIKDHDLTTEEVKTLC 300

Query: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATSTSVKDAENEVKQDDDDKILNEMEQLTF 360
           DDLRVLGKQDFKHLLKWRLHIRKALSP+QKATSTSVKDAENEVKQDDDDKILNEMEQLTF
Sbjct: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPSQKATSTSVKDAENEVKQDDDDKILNEMEQLTF 360

Query: 361 NMERKKRREKKRLAKRIAKDKARKATGMQRDVMDDSYGDPDLFSLSSIKDKKGLRGIDST 420
           NMERKKRREKKRLAKRIAKDKARKATGMQRDVMDDSYGDPDLFSLSSIKDKKGLRGIDST
Sbjct: 361 NMERKKRREKKRLAKRIAKDKARKATGMQRDVMDDSYGDPDLFSLSSIKDKKGLRGIDST 420

Query: 421 EYDDNVEVGEYENHVAKVENDGSSASDIDSDEERRRYDERMETLLDQAYESFVARKEGST 480
           EYDDNVEVGEYENHVAKVENDGSSASDIDSDEERRRYDERME+LLDQAYESFVARKEGST
Sbjct: 421 EYDDNVEVGEYENHVAKVENDGSSASDIDSDEERRRYDERMESLLDQAYESFVARKEGST 480

Query: 481 KQRKRAKKAYSDDAEVLEEDENGGDGIQSDYDSDENLVDEDKNPLMVSLDDGEAPTQEEI 540
           KQRKRAKKAYSDDAEVLEEDENGGDGIQSDYDSDENLVDEDKNPLMVSLDDGEAPTQEEI
Sbjct: 481 KQRKRAKKAYSDDAEVLEEDENGGDGIQSDYDSDENLVDEDKNPLMVSLDDGEAPTQEEI 540

Query: 541 ANKWFSQDIFAEAAQEGDMEMLDSEDDMQVDEPKERTSVSKEAKSNISKNAREKSNISKN 600
           ANKWFSQDIFAEAAQEGDMEMLDSEDDMQVDEPKE T VSKEAKSNISKNAREKSNISKN
Sbjct: 541 ANKWFSQDIFAEAAQEGDMEMLDSEDDMQVDEPKEITCVSKEAKSNISKNAREKSNISKN 600

Query: 601 ARETDKADDGFEVVPAPATDSSDDSSSSEDEDPETKAEILACAKKMLRKKQREQILDDSY 660
           ARETDKADDGFEVVPAPATDSSDDSSSSEDEDPETKAEILACAKKMLRKKQREQILDDSY
Sbjct: 601 ARETDKADDGFEVVPAPATDSSDDSSSSEDEDPETKAEILACAKKMLRKKQREQILDDSY 660

Query: 661 NKYMFDDAGLPNWFLDEERRHRIPIKPVTKEEIAAMRAQFKEINARPAKKVAEAKARKKR 720
           NKYMFDDAGLPNWFLDEERRHRIPIKPVTKEEIAAMRAQFKEINARPAKKVAEAKARKKR
Sbjct: 661 NKYMFDDAGLPNWFLDEERRHRIPIKPVTKEEIAAMRAQFKEINARPAKKVAEAKARKKR 720

Query: 721 VAMKKLEKVRKKANAISDQADINDRSKRKMIDQLYKKAVPQRPKKEFVVAKKGVQVKVGK 780
           VAMKKLEKVRKKANAISDQADINDRSKRKMIDQLYKKAVPQRPKKEFVVAKKGVQVKVGK
Sbjct: 721 VAMKKLEKVRKKANAISDQADINDRSKRKMIDQLYKKAVPQRPKKEFVVAKKGVQVKVGK 780

Query: 781 GKVRVDRRMKKDARKHGMSKQGKGSKKGKNLKAQRGKGGSAKASSAKKG 830
           GKVRVDRRMKKDARKHGMSKQGKGSKKGKNLKAQRGKGGSAKASSAKKG
Sbjct: 781 GKVRVDRRMKKDARKHGMSKQGKGSKKGKNLKAQRGKGGSAKASSAKKG 829

BLAST of MS020541 vs. ExPASy TrEMBL
Match: A0A6J1FTB8 (Putative rRNA methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111448289 PE=3 SV=1)

HSP 1 Score: 1361.3 bits (3522), Expect = 0.0e+00
Identity = 730/830 (87.95%), Postives = 776/830 (93.49%), Query Frame = 0

Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSAHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRS+HAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMNEKGCPAFDLVLHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGA+AIEQDITKPECKARLKKIMNEKGC AFDL+LHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGS 120

Query: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPRGTFVTKVFRSQDYSSVLYCLKQLFEKVE 180
           PNVGGAWAQEAMSQNSLVIDSVKLATQLLAP+G FVTKVFRSQDYSSVLYCLKQLFEKVE
Sbjct: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVE 180

Query: 181 VDKPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH 240
           VDKPAASRSASAEIYVLGLRY APAKIDPRLLDVKHLFQGSVE QRKVVDVLRGTKQKRH
Sbjct: 181 VDKPAASRSASAEIYVLGLRYTAPAKIDPRLLDVKHLFQGSVESQRKVVDVLRGTKQKRH 240

Query: 241 RDGYEDGDMTLRKVSSASNFIWSDSPLEILGSVTSITFDDSACLPIKDHDLTTEEVKTLC 300
           RDGYEDGD+TLRKVSSASN+IWSDSPLEILG+VT ITFDD ACL IK+HDLTTEEVK LC
Sbjct: 241 RDGYEDGDLTLRKVSSASNYIWSDSPLEILGTVTCITFDDPACLLIKNHDLTTEEVKALC 300

Query: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATSTSVKDAENEVKQDDDDKILNEMEQLTF 360
           DDLRVLGKQDFKHLLKWRLHIRKALSPNQKAT TSVKDAENEVKQ++DDK+LNEME+LT+
Sbjct: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATLTSVKDAENEVKQNEDDKLLNEMEELTY 360

Query: 361 NMERKKRREKKRLAKRIAKDKARKATGMQRDVMDDSYGDPDLFSLSSIKDKKGLRGIDST 420
            +ERKK+REKK LAKR AKDKARKA GMQ DVM++ Y D +LFSLS+IK K  LR +DST
Sbjct: 361 AIERKKKREKKLLAKRRAKDKARKAMGMQLDVMEEGYIDHELFSLSNIKGKNDLRVVDST 420

Query: 421 EYD-DNVEVGEYENHVAKVENDGSSASDIDSDEERRRYDERMETLLDQAYESFVARKEGS 480
           +YD DN E+GE+EN V   E+ GSSASDIDSDEERRRYDE ME LLDQAYESFVARKEGS
Sbjct: 421 DYDVDNGELGEHENDVTNEEDHGSSASDIDSDEERRRYDEHMEELLDQAYESFVARKEGS 480

Query: 481 TKQRKRAKKAYSDDAEVLEEDENGGDGIQSDYDSDENLVDEDKNPLMVSLDDGEAPTQEE 540
            KQRKRAKKAYSDDAE+LEEDENGGDG QSDYDSDENLVDED+NPLMVSLDDG  PTQEE
Sbjct: 481 AKQRKRAKKAYSDDAELLEEDENGGDGFQSDYDSDENLVDEDRNPLMVSLDDGGEPTQEE 540

Query: 541 IANKWFSQDIFAEAAQEGDMEMLDSEDDMQVDEPKERTSVSKEAKSNISKNAREKSNISK 600
           IANKWF QDIFAEAA++GD++ LDSEDDMQVD PKE+T+V+K AKSNISKNAREKS IS 
Sbjct: 541 IANKWFGQDIFAEAAEDGDLKELDSEDDMQVDGPKEKTAVTKVAKSNISKNAREKSKIST 600

Query: 601 NARETDKADDGFEVVPAPATDSSDDSSS--SEDEDPETKAEILACAKKMLRKKQREQILD 660
           NA+++ KADDGFEVVPAPATDSS+DSSS  SEDE+P+TKAEILACAKKM+RKKQREQILD
Sbjct: 601 NAKDSIKADDGFEVVPAPATDSSNDSSSEESEDEEPDTKAEILACAKKMVRKKQREQILD 660

Query: 661 DSYNKYMFDDAGLPNWFLDEERRHRIPIKPVTKEEIAAMRAQFKEINARPAKKVAEAKAR 720
           DSYNKYMFDD GLP WFLDEE+RHR PIKP+TKEE+AAMRAQFKEI+ARPAKKVAEAKAR
Sbjct: 661 DSYNKYMFDDTGLPKWFLDEEKRHRQPIKPITKEEVAAMRAQFKEIDARPAKKVAEAKAR 720

Query: 721 KKRVAMKKLEKVRKKANAISDQADINDRSKRKMIDQLYKKAVPQRPKKEFVVAKKGVQVK 780
           KKRVAMKKLEKVRKKAN ISDQADI+DRSK KMIDQLYKKAVPQRPKKEFVVAKKGVQV+
Sbjct: 721 KKRVAMKKLEKVRKKANIISDQADISDRSKSKMIDQLYKKAVPQRPKKEFVVAKKGVQVR 780

Query: 781 VGKGKVRVDRRMKKDARKHGMSKQGKGSKKGKNLKAQRGKGGSAKASSAK 828
           VGKGK  VDRRMKKDARKHGMSKQGKGSKKGKN KA RGKGGSAKAS  K
Sbjct: 781 VGKGKTLVDRRMKKDARKHGMSKQGKGSKKGKNSKAPRGKGGSAKASGKK 830

BLAST of MS020541 vs. ExPASy TrEMBL
Match: A0A6J1G7D4 (Putative rRNA methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111451531 PE=3 SV=1)

HSP 1 Score: 1355.9 bits (3508), Expect = 0.0e+00
Identity = 734/835 (87.90%), Postives = 775/835 (92.81%), Query Frame = 0

Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSAHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRS+HAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMNEKGCPAFDLVLHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGA+AIEQDIT+PECKARLKKIMNEKGC AFDLVLHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGALAIEQDITRPECKARLKKIMNEKGCAAFDLVLHDGS 120

Query: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPRGTFVTKVFRSQDYSSVLYCLKQLFEKVE 180
           PNVGGAWAQEAMSQNSLVIDSVKLATQLLAP+GTFVTKVFRSQDYSSVLYCLKQLFEKVE
Sbjct: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGTFVTKVFRSQDYSSVLYCLKQLFEKVE 180

Query: 181 VDKPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH 240
           VDKPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH
Sbjct: 181 VDKPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH 240

Query: 241 RDGYEDGDMTLRKVSSASNFIWSDSPLEILGSVTSITFDDSACLPIKDHDLTTEEVKTLC 300
           RDGYEDGDMTLRK SSASNFIWSDSPLEILG+VT+ITF+D ACLPIKDH+LTTEEVKTLC
Sbjct: 241 RDGYEDGDMTLRKESSASNFIWSDSPLEILGTVTTITFNDPACLPIKDHELTTEEVKTLC 300

Query: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATSTSVKDAENEVKQDDDDKILNEMEQLTF 360
           DDL VLGKQDFKHLLKWRLHIRKALSP QK TSTSV DAENEVKQD+DDK+LNEME+LT+
Sbjct: 301 DDLGVLGKQDFKHLLKWRLHIRKALSPKQKVTSTSVNDAENEVKQDEDDKLLNEMEELTY 360

Query: 361 NMERKKRREKKRLAKRIAKDKARKATGMQRDVMDDSYGDPDLFSLSSIKDKKGLRGIDST 420
            MERKK+REKK LAKR AKDKARKA GMQ DVM+D Y D +LFSLS+IK K  LR +DST
Sbjct: 361 AMERKKKREKKLLAKRKAKDKARKALGMQLDVMEDGYIDHELFSLSNIKGKNDLRVVDST 420

Query: 421 EY-DDNVEVGEYENHVAKVENDGSSASDIDSDEERRRYDERMETLLDQAYESFVARKEGS 480
           EY DDN E+GE EN V K EN GSS SDIDSDEERRRYD  ME LLDQAYESFVARKEGS
Sbjct: 421 EYDDDNDEMGEQENGVTKEENHGSSDSDIDSDEERRRYDAHMEELLDQAYESFVARKEGS 480

Query: 481 TKQRKRAKKAYSDDAEVLEEDENGGDGIQSDYDSDENLVDEDKNPLMVSLDDGEAPTQEE 540
           TKQRKRAKKAYSDDAE+L+EDE+GGDG  SDYDSDENLVDEDKNPLMVSLDDG  PT+EE
Sbjct: 481 TKQRKRAKKAYSDDAELLKEDESGGDG-PSDYDSDENLVDEDKNPLMVSLDDGGVPTEEE 540

Query: 541 IANKWFSQDIFAEAAQEGDM-EMLDSEDDMQVDEPKERTSVSKEAKSNISKNAREKSNIS 600
           IANKWFSQDIFAEA ++GD+ E++DSEDDMQ+D PKE+ +VSKEAK NISK  REKS  S
Sbjct: 541 IANKWFSQDIFAEAVEDGDLKELVDSEDDMQIDRPKEKAAVSKEAKPNISKKEREKSKTS 600

Query: 601 KNARETDKADDGFEVVPAPATDSSDDSSS--SEDEDPETKAEILACAKKMLRKKQREQIL 660
            NARETDK DDGFEVVPAPATDSSDDSSS  SEDEDP+TKAEILACAKKMLRKKQREQIL
Sbjct: 601 TNARETDKEDDGFEVVPAPATDSSDDSSSDESEDEDPDTKAEILACAKKMLRKKQREQIL 660

Query: 661 DDSYNKYMFDDAGLPNWFLDEERRHRIPIKPVTKEEIAAMRAQFKEINARPAKKVAEAKA 720
           DD+YNKYMFDD GLP+WFLDEERRHR PIKP+TKEE+AAM+AQFKEI+ARPAKKVAEAKA
Sbjct: 661 DDAYNKYMFDDTGLPSWFLDEERRHRQPIKPITKEEVAAMKAQFKEIDARPAKKVAEAKA 720

Query: 721 RKKRVAMKKLEKVRKKANAISDQADINDRSKRKMIDQLYKKAVPQRPKKEFVVAKKGVQV 780
           RKKRVAMKKLEKVRKKAN+ISDQADI+DRSKRKMIDQLYKKAVP+RPKKEFVVAKKGVQV
Sbjct: 721 RKKRVAMKKLEKVRKKANSISDQADISDRSKRKMIDQLYKKAVPERPKKEFVVAKKGVQV 780

Query: 781 KVGKGKVRVDRRMKKDARKHGMSKQGKGSK----KGKNLKAQRGKGGSAKASSAK 828
           +VGKGKV VDRRMKKD RKHGMSKQGKGSK    KGKN KA RGKGG AKAS  K
Sbjct: 781 RVGKGKVLVDRRMKKDMRKHGMSKQGKGSKKGKGKGKNSKAPRGKGGPAKASGKK 834

BLAST of MS020541 vs. ExPASy TrEMBL
Match: A0A6J1L3E5 (Putative rRNA methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111499543 PE=3 SV=1)

HSP 1 Score: 1349.3 bits (3491), Expect = 0.0e+00
Identity = 729/831 (87.73%), Postives = 772/831 (92.90%), Query Frame = 0

Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSAHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRS+HAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMNEKGCPAFDLVLHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGA+AIEQDIT+PECKARLKKIMNEKGC AFDLVLHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGALAIEQDITRPECKARLKKIMNEKGCAAFDLVLHDGS 120

Query: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPRGTFVTKVFRSQDYSSVLYCLKQLFEKVE 180
           PNVGGAWAQEAMSQNSLVIDSVKLATQLLAP+GTFVTKVFRSQDYSSVLYCLKQLFEKVE
Sbjct: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGTFVTKVFRSQDYSSVLYCLKQLFEKVE 180

Query: 181 VDKPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH 240
           VDKPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH
Sbjct: 181 VDKPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH 240

Query: 241 RDGYEDGDMTLRKVSSASNFIWSDSPLEILGSVTSITFDDSACLPIKDHDLTTEEVKTLC 300
           RDGYEDGDMTLRK SSASNFIWSDSPLEILG+VT+ITF+D ACL IKDH+LTTEEVKTLC
Sbjct: 241 RDGYEDGDMTLRKESSASNFIWSDSPLEILGTVTTITFNDPACLLIKDHELTTEEVKTLC 300

Query: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATSTSVKDAENEVKQDDDDKILNEMEQLTF 360
           DDL VLGKQDFKHLLKWRLHIRKALSP QK TSTSV DAENEVKQD+DDK+LNEME+LT+
Sbjct: 301 DDLGVLGKQDFKHLLKWRLHIRKALSPKQKVTSTSVNDAENEVKQDEDDKLLNEMEELTY 360

Query: 361 NMERKKRREKKRLAKRIAKDKARKATGMQRDVMDDSYGDPDLFSLSSIKDKKGLRGIDST 420
            MERKK+REKK LAKR AKDKARKA GMQ DVM+D Y D +LFSLS+IK K  LR +DST
Sbjct: 361 AMERKKKREKKLLAKRKAKDKARKALGMQLDVMEDGYIDHELFSLSNIKGKNDLRVVDST 420

Query: 421 EYDD-NVEVGEYENHVAKVENDGSSASDIDSDEERRRYDERMETLLDQAYESFVARKEGS 480
           EYDD N E+GE E  V K EN GSS SD+DSDEERRRYD  +E LLDQAY+SFVARKEGS
Sbjct: 421 EYDDENDEMGEQEIGVTKEENHGSSDSDLDSDEERRRYDAHIEELLDQAYDSFVARKEGS 480

Query: 481 TKQRKRAKKAYSDDAEVLEEDENGGDGIQSDYDSDENLVDEDKNPLMVSLDDGEAPTQEE 540
           TKQRKRAKKAYSDDAE+L+EDENGGDG  SDYDSDENLVDEDKNPLMVSLD G  PT+EE
Sbjct: 481 TKQRKRAKKAYSDDAELLKEDENGGDG-PSDYDSDENLVDEDKNPLMVSLDYGGVPTEEE 540

Query: 541 IANKWFSQDIFAEAAQEGDM-EMLDSEDDMQVDEPKERTSVSKEAKSNISKNAREKSNIS 600
           +ANKWFSQDIFAEA ++GD+ E++DSEDDMQVD PKE+ +VSKEAK NISK  REK   S
Sbjct: 541 VANKWFSQDIFAEAVEDGDLKELVDSEDDMQVDRPKEKAAVSKEAKPNISKKEREKLKTS 600

Query: 601 KNARETDKADDGFEVVPAPATDSSDDSSS--SEDEDPETKAEILACAKKMLRKKQREQIL 660
            NARETDK DDGFEVVPAPATDSSDDSSS  SEDEDP+TKAEILACAKKMLRKKQREQIL
Sbjct: 601 TNARETDKGDDGFEVVPAPATDSSDDSSSDESEDEDPDTKAEILACAKKMLRKKQREQIL 660

Query: 661 DDSYNKYMFDDAGLPNWFLDEERRHRIPIKPVTKEEIAAMRAQFKEINARPAKKVAEAKA 720
           DD+YNKYMFDD GLP+WFLDEERRHR PIKP+TKEE+AAM+AQFKEI+ARPAKKVAEAKA
Sbjct: 661 DDAYNKYMFDDTGLPSWFLDEERRHRRPIKPITKEEVAAMKAQFKEIDARPAKKVAEAKA 720

Query: 721 RKKRVAMKKLEKVRKKANAISDQADINDRSKRKMIDQLYKKAVPQRPKKEFVVAKKGVQV 780
           RKKRVAMKKLEKVRKKAN+ISDQADI+DRSKRKMIDQLYKKAVP+RPKKEFVVAKKGVQV
Sbjct: 721 RKKRVAMKKLEKVRKKANSISDQADISDRSKRKMIDQLYKKAVPERPKKEFVVAKKGVQV 780

Query: 781 KVGKGKVRVDRRMKKDARKHGMSKQGKGSKKGKNLKAQRGKGGSAKASSAK 828
           +VGKGKV VDRRMKKD RKHGMSKQGKGSKKGKN KA RGKGGSAKAS  K
Sbjct: 781 RVGKGKVLVDRRMKKDMRKHGMSKQGKGSKKGKNSKAPRGKGGSAKASGKK 830

BLAST of MS020541 vs. ExPASy TrEMBL
Match: A0A5A7TQD1 (Putative rRNA methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold303G00370 PE=3 SV=1)

HSP 1 Score: 1333.2 bits (3449), Expect = 0.0e+00
Identity = 715/832 (85.94%), Postives = 768/832 (92.31%), Query Frame = 0

Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSAHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRS+HAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMNEKGCPAFDLVLHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIM+EKGC AFDL+LHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMSEKGCAAFDLILHDGS 120

Query: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPRGTFVTKVFRSQDYSSVLYCLKQLFEKVE 180
           PNVGGAWAQEAMSQNSLVIDSV+LATQLLAP+GTFVTKVFRSQDYSSVLYC+KQLFEKVE
Sbjct: 121 PNVGGAWAQEAMSQNSLVIDSVRLATQLLAPKGTFVTKVFRSQDYSSVLYCIKQLFEKVE 180

Query: 181 VDKPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH 240
           VDKPAASRSASAEIY+LG+RYKAPAKIDPRLLDVKHLFQGSVEPQ+KVVDVLRGTKQKRH
Sbjct: 181 VDKPAASRSASAEIYILGIRYKAPAKIDPRLLDVKHLFQGSVEPQQKVVDVLRGTKQKRH 240

Query: 241 RDGYEDGDMTLRKVSSASNFIWSDSPLEILGSVTSITFDDSACLPIKDHDLTTEEVKTLC 300
           RDGYEDG   LRKVSSASNFIWSDSPLEILG+VT ITFD+   LPIKDHDLTTEEVK LC
Sbjct: 241 RDGYEDGATILRKVSSASNFIWSDSPLEILGTVTCITFDEPDSLPIKDHDLTTEEVKALC 300

Query: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATSTSVKDAENEVKQDDDDKILNEMEQLTF 360
           DDLRVLGKQDFKHLLKWRLHIRKALSP+QKATSTSVKD ENEVKQD+DDK+LNEME+L +
Sbjct: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPDQKATSTSVKDVENEVKQDEDDKLLNEMEELAY 360

Query: 361 NMERKKRREKKRLAKRIAKDKARKATGMQRDVMDDSYGDPDLFSLSSIKDKKGLRGIDST 420
            MERKK+R KK LAKR AKDKARKA G Q DVM++ Y D +LFSLS+IK K  L+ +DST
Sbjct: 361 AMERKKKRTKKLLAKRKAKDKARKAMGTQIDVMEEGYVDHELFSLSNIKGKNDLKAVDST 420

Query: 421 EY-DDNVEVGEYENHVAKVENDGSSASDIDSDEERRRYDERMETLLDQAYESFVARKEGS 480
           EY DDN E+GE+EN V K EN GSSASDIDSDEERRRYDE ME LLDQAYESFV+RKEGS
Sbjct: 421 EYDDDNGELGEHENDVTKEENRGSSASDIDSDEERRRYDEHMEELLDQAYESFVSRKEGS 480

Query: 481 TKQRKRAKKAYSDDAEVLEEDENGGDGIQSDYDSDENLVDEDKNPLMVSLDDGEAPTQEE 540
            K+RKR K AYSD+AE+LEEDENGGDGIQSDYDSDEN+VD DKNPLMVSLDDG  PTQEE
Sbjct: 481 AKRRKRVKNAYSDNAELLEEDENGGDGIQSDYDSDENIVDADKNPLMVSLDDGAEPTQEE 540

Query: 541 IANKWFSQDIFAEAAQEGDMEMLDSEDDMQVDEPKERTSVSKEAKSNISKNAREKSNISK 600
           IA+KWFSQDIFAEA +EGD++ LDS+DDM+VD PKE  +VSK+AKS+IS+NA E S IS 
Sbjct: 541 IASKWFSQDIFAEAVEEGDLKGLDSDDDMEVDGPKETLAVSKKAKSSISQNAGENSKISS 600

Query: 601 NARETDKADDGFEVVPAPATDSSDDSSS--SEDEDPETKAEILACAKKMLRKKQREQILD 660
           NARE+++ DDGFEVVPAPATDSSD SSS  S+DEDP+T+AEILACAKKMLRKKQREQILD
Sbjct: 601 NARESNEVDDGFEVVPAPATDSSDSSSSEESDDEDPDTRAEILACAKKMLRKKQREQILD 660

Query: 661 DSYNKYMFDDAGLPNWFLDEERRHRIPIKPVTKEEIAAMRAQFKEINARPAKKVAEAKAR 720
           DSYNKYMFDDAGLP WFLDEERRHR PIKPVTKEE+AA+RAQFKEI+ARPAKKVAEAKAR
Sbjct: 661 DSYNKYMFDDAGLPKWFLDEERRHRQPIKPVTKEEVAAIRAQFKEIDARPAKKVAEAKAR 720

Query: 721 KKRVAMKKLEKVRKKANAISDQADINDRSKRKMIDQLYKKAVPQRPKKEFVVAKKGVQVK 780
           KKRVAMKKLEKVRKKAN ISDQADI+DRSKRKMIDQLYKKAVPQRPKKE VVAKKGVQV+
Sbjct: 721 KKRVAMKKLEKVRKKANVISDQADISDRSKRKMIDQLYKKAVPQRPKKELVVAKKGVQVR 780

Query: 781 VGKGKVRVDRRMKKDARKHGMSKQGKGSKKGKNLKAQRGKGGSAKASSAKKG 830
           VGKGKV VDRRMKKDARKHGMSKQGKGSKKGKN KA R KGG AK S+  +G
Sbjct: 781 VGKGKVLVDRRMKKDARKHGMSKQGKGSKKGKNSKAPRAKGGPAKDSNTPRG 832

BLAST of MS020541 vs. TAIR 10
Match: AT4G25730.1 (FtsJ-like methyltransferase family protein )

HSP 1 Score: 949.5 bits (2453), Expect = 1.8e-276
Identity = 529/838 (63.13%), Postives = 658/838 (78.52%), Query Frame = 0

Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSAHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKE G+RSRAS+KL QLD+KY+ L SAHAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKERGFRSRASYKLLQLDAKYSLLHSAHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMNEKGCPAFDLVLHDGS 120
           VE+VPVGSLV+G+DLVPI PVRG V + QDIT+ ECK+++K++M + G  AF+LVLHDGS
Sbjct: 61  VEKVPVGSLVLGIDLVPILPVRGCVTMTQDITRTECKSKIKQVMEQHGVSAFNLVLHDGS 120

Query: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPRGTFVTKVFRSQDYSSVLYCLKQLFEKVE 180
           PNVGGAWAQEAMSQN+LVIDSV+LAT+ LA  G  VTKVFRS+DY+SVLYCL +LFEKVE
Sbjct: 121 PNVGGAWAQEAMSQNALVIDSVRLATEFLARNGNLVTKVFRSRDYNSVLYCLGRLFEKVE 180

Query: 181 VDKPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH 240
           V KP ASRSASAE Y++GL+Y APAKIDPRLLD +HLF+ S EP RKVVDVL G+KQKR+
Sbjct: 181 VFKPPASRSASAETYLVGLKYLAPAKIDPRLLDYRHLFKESAEPTRKVVDVLGGSKQKRN 240

Query: 241 RDGYEDGDMTLRKVSSASNFIWSDSPLEILGSVTSITFDDSACLPIKDHDLTTEEVKTLC 300
           RDGYEDG+  LR+V+SA++FIWS++PL++LG+ TSI+FDD A LP+K+HDLTTEE+K LC
Sbjct: 241 RDGYEDGESILRRVASAADFIWSENPLDVLGTTTSISFDDQASLPLKEHDLTTEEIKILC 300

Query: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATSTSVKDAENEVKQDDDDKILNEMEQLTF 360
           DDL VLGK DFKH+LKWR+ IRKAL+P +K  +    D   E ++++DDK+LNE+E+LT 
Sbjct: 301 DDLPVLGKNDFKHILKWRMQIRKALTPEKKEVAKPEPDVGKEDEENEDDKLLNELEELTN 360

Query: 361 NMERKKRREKKRLAKRIAKDKARKATGMQRDVMDDSYGDPDLFSLSSIKDKKGLRGIDST 420
            ++RKK++ KK LAKR AKDKARKATG Q DV++D + D +LFSL++IK KK L  +D+ 
Sbjct: 361 TVDRKKKQAKKILAKRRAKDKARKATGPQMDVLEDGFVDNELFSLNAIKGKKDLMAVDND 420

Query: 421 EYDD-NVEVGEYENHVAKVENDGSSASDIDSDEERRRYDERMETLLDQAYESFVARKEGS 480
           E D+ N    E E+H  +  +D S  SD DSDEER++Y E+ME + +QAYE ++ +KEGS
Sbjct: 421 EDDNGNAVDSENEDH-GEGASDDSKDSDRDSDEERQKYTEQMEEIFEQAYERYMVKKEGS 480

Query: 481 TKQRKRAKKAYSDDAEVLEEDENGGDGIQSDYDSDENLVDEDKNPLMVSLDDGEAPTQEE 540
            KQRKRA++A+   AE LEE + G + ++ DYDSD N   ++ NPL+V LDDG   T+EE
Sbjct: 481 AKQRKRARQAH---AEKLEEGD-GDEEMKIDYDSDMNEEKDEANPLVVPLDDGVVQTKEE 540

Query: 541 IANKWFSQDIFAEAAQEGDMEMLDSEDDMQVDEPKERTSVSKEAKSNISKNAREKSNISK 600
           I+N+WFSQ+IFAEA +EGD+   DSED++           +K+   N+SK  + K   SK
Sbjct: 541 ISNQWFSQNIFAEAVEEGDLGKDDSEDEI----------ANKKKSKNLSKPDKSKQKASK 600

Query: 601 NARETD-------KADDGFEVVPAPATDSSDDSSSSEDEDPETKAEILACAKKMLRKKQR 660
            +  +D       K +D FEVVPAPATDS  DS SS ++D  TKAEILACAKKMLRKKQR
Sbjct: 601 ASVLSDQSLPNSSKKEDEFEVVPAPATDS--DSDSSSEDDVHTKAEILACAKKMLRKKQR 660

Query: 661 EQILDDSYNKYMFDDAGLPNWFLDEERRHRIPIKPVTKEEIAAMRAQFKEINARPAKKVA 720
           EQ+LDD+YNK+MF D GLP WF+D+E++HR P+KPVTK+E+ AM+AQFKEINARPAKKVA
Sbjct: 661 EQMLDDAYNKHMFVDEGLPKWFVDDEKQHRQPMKPVTKDEVNAMKAQFKEINARPAKKVA 720

Query: 721 EAKARKKRVAMKKLEKVRKKANAISDQADINDRSKRKMIDQLYKKAV-PQRPKKEFVVAK 780
           EAKARKKR A K+LEKVRKKAN ISD ADI+DRSK KMID+LYKKA  P++P+KE VV+K
Sbjct: 721 EAKARKKRAAQKRLEKVRKKANTISDTADISDRSKDKMIDKLYKKAAEPRKPRKELVVSK 780

Query: 781 KGVQVKVGKGKVRVDRRMKKDARKHGMSKQGKGSKKGKNLKAQRGKGGSAKASSAKKG 830
           KGV VKVGKG+ RVDRRMK D RK G  K G+  +KG     Q+GK  + K    K G
Sbjct: 781 KGVGVKVGKGQKRVDRRMKSDDRKRGGGKPGRNGQKGTGKAGQKGKRPAGKPRGRKPG 821

BLAST of MS020541 vs. TAIR 10
Match: AT5G01230.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 135.2 bits (339), Expect = 2.4e-31
Identity = 82/223 (36.77%), Postives = 125/223 (56.05%), Query Frame = 0

Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSAHAVLDLCAAPGGWMQVA 60
           MGK   + + D YYR AKE G+R+R+++KL Q+D ++N       V+DLCAAPG W QV 
Sbjct: 1   MGKA-SRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVL 60

Query: 61  VER--VPVGS----------LVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMNEKG 120
             +  +P  S          L+V +DL P+AP+ G + ++ DIT    +     I +  G
Sbjct: 61  SRQLYLPAKSSAESKDGDLPLIVAIDLQPMAPIEGVIQVQGDIT--NARTAEVVIRHFDG 120

Query: 121 CPAFDLVLHDGSPNVGGAWAQEAMSQNSLVIDSVKLATQLLAPRGTFVTKVFRSQDYSSV 180
           C A DLV+ DG+P+V G    +   Q+ L++  + + T +L   G F+ K+FR +D +S+
Sbjct: 121 CKA-DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHILKEGGKFIAKIFRGKD-TSL 180

Query: 181 LYC-LKQLFEKVEVDKPAASRSASAEIYVLGLRYKAPAKIDPR 211
           LYC LK  F  V   KP +SR++S E + +   Y  P   +PR
Sbjct: 181 LYCQLKLFFPTVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPR 218

BLAST of MS020541 vs. TAIR 10
Match: AT5G13830.1 (FtsJ-like methyltransferase family protein )

HSP 1 Score: 68.6 bits (166), Expect = 2.8e-11
Identity = 58/224 (25.89%), Postives = 100/224 (44.64%), Query Frame = 0

Query: 11  DKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSAHAVLDLCAAPGGWMQVAVERV-PV--G 70
           D +YR A+  GY +R+++KL Q+  +Y  ++   +VLDL  APG W+QVA + + P+  G
Sbjct: 8   DFFYREAQRLGYVARSAFKLLQIQKQYKLIKPGSSVLDLGCAPGAWLQVACQSLGPLRSG 67

Query: 71  SLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMNE----------KGCP---AFDL 130
            +VVG+D+  +               P+C +R++ I  +          +  P    F +
Sbjct: 68  GIVVGMDIKKVK------------VPPQCDSRVQTIAADVLNFPRQKIRELSPQQLGFSV 127

Query: 131 VLHDGSPNVGGAWAQEAMSQNSLVIDSVKLAT---------------------QLLAPRG 190
           +L D   +V G   ++A     L + ++ LA                       +L   G
Sbjct: 128 ILSDMCHSVSGITTRDAALSAELGMRALDLAVGQAAISQSPNDDDGGPNESRPGVLRHGG 187

Query: 191 TFVTKVFRSQDYSSVLYCLKQLFEKVEVDKPAASRSASAEIYVL 198
             V K+  S+D        K +F K    +P A+R +S EIY++
Sbjct: 188 HLVIKLLESEDAQDFARICKPIFNKASWLRPKATRPSSREIYLI 219

BLAST of MS020541 vs. TAIR 10
Match: AT5G01230.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 61.2 bits (147), Expect = 4.4e-09
Identity = 28/58 (48.28%), Postives = 39/58 (67.24%), Query Frame = 0

Query: 1  MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSAHAVLDLCAAPGGWMQ 59
          MGK   + + D YYR AKE G+R+R+++KL Q+D ++N       V+DLCAAPG W Q
Sbjct: 1  MGKA-SRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQ 57