MS020539 (gene) Bitter gourd (TR) v1

Overview


Name	MS020539
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Adenine nucleotide alpha hydrolases-like superfamily protein
Location	scaffold375: 1012121 .. 1013416 (-)
RNA-Seq Expression	MS020539
Synteny	MS020539

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGACATTGATGGAGGATGAGGAGGAATACAGCTGGAGAGAAGTGCGGCTGCCGTCTCTGATCCCGGTGGTGCCGGAGCCGGAGCTGGAGAGAGAGACGGCGGAGAGACGCCGCGGGCGAGACATCCTGGTGGCCGTCGATCACGGACCCAACAGCAAACACGCCTTGGATTGGGCTCTCGTCCATTTCTGCCGCCTCGCCGACACCATCCATCTCGTCCACGCCGTTTCCAGTAACTACTCTCAAACCAAAATTTTCCCATTTCCCAGATTCCATTCTCAAATTTTGTTTTGTGATTCTGATCTGTTTGTTTTGTAGATGTGAAGAACGAATTGGTTTATGAGGCGAGCCAGGGGCTGATGGAGAAGCTTGCGGTGGAGGCCTTCGAGGTGGCCATGGTCAGGACTGTGGCTCGGATTGTGCAGGGAGATGCAGGGAAGGTTGTCTGTAAAGAAGCAGAGAAGTTGAAGCCTGCTGCTGTTGTTATGGGCACCAGAGGAAGAAGCTTGATTCAAAGGTATAACCACATAACTTCGTCCAAAATTTTACCATTTTAAGCTTCAAATTTGAATACCATTTGGTTCTTCTCTCTTGATACATGTGTTTTTGTTTTAGGATAATATAAGTTTTGGGAGTGCATTTAAAATTTTGGATGGCTGCACTACAAAGGGAATCTAGGCTTACTTTTATTCAAAAGAGAGAAAACTATATATATATTTTGTATATTTGTGCAAGTGTCCGAACTAGTTTACTGCATTTGACGGTTGTCAGTTAAACTTGTAGGTAGTTGCTATGGTTTGAACTTATTCTCCGAACACCTACCTTTTCAGTGCTCCCAATGACTACTAGGCTAACTCACAATAGTTCATAGACATATACTATAGGAGTATTTGACTTAAGTCGGGTTAAAAAGTTTTAAGTTTGGTAGATGTATTTATCTCAACTAGCTACATAACTTTTGACTTATAAAACTCTATAATGGTGAAGTACTGTTTTGGTGCGGTCTGCGAGAGCTATATCAAACCAATATAAACTTAAACTAGATCTGATGAGGGATTAACTTCATCGTCAAGAGGGAAACAACTTGGAGAATTGTATGCTACACGAGTTGTTATTAAATTTATGGTTTAAAATACTATCTTAGTCCCCGTACCTTCGATTTTGGTTTATTTTAGGCTCTATATTTTTAAGACTTTTTTTTTCAGTGTTTTGCAGGGAAGTGTGAGTGAATATGTGTTCCACAACTGCAAATCAGCACCTGTTATAATAGTTCCTGGGAAAGGTACTATTTTG

mRNA sequence

ATGGAGACATTGATGGAGGATGAGGAGGAATACAGCTGGAGAGAAGTGCGGCTGCCGTCTCTGATCCCGGTGGTGCCGGAGCCGGAGCTGGAGAGAGAGACGGCGGAGAGACGCCGCGGGCGAGACATCCTGGTGGCCGTCGATCACGGACCCAACAGCAAACACGCCTTGGATTGGGCTCTCGTCCATTTCTGCCGCCTCGCCGACACCATCCATCTCGTCCACGCCGTTTCCAATGTGAAGAACGAATTGGTTTATGAGGCGAGCCAGGGGCTGATGGAGAAGCTTGCGGTGGAGGCCTTCGAGGTGGCCATGGTCAGGACTGTGGCTCGGATTGTGCAGGGAGATGCAGGGAAGGTTGTCTGTAAAGAAGCAGAGAAGTTGAAGCCTGCTGCTGTTGTTATGGGCACCAGAGGAAGAAGCTTGATTCAAAGTGTTTTGCAGGGAAGTGTGAGTGAATATGTGTTCCACAACTGCAAATCAGCACCTGTTATAATAGTTCCTGGGAAAGGTACTATTTTG

Coding sequence (CDS)

ATGGAGACATTGATGGAGGATGAGGAGGAATACAGCTGGAGAGAAGTGCGGCTGCCGTCTCTGATCCCGGTGGTGCCGGAGCCGGAGCTGGAGAGAGAGACGGCGGAGAGACGCCGCGGGCGAGACATCCTGGTGGCCGTCGATCACGGACCCAACAGCAAACACGCCTTGGATTGGGCTCTCGTCCATTTCTGCCGCCTCGCCGACACCATCCATCTCGTCCACGCCGTTTCCAATGTGAAGAACGAATTGGTTTATGAGGCGAGCCAGGGGCTGATGGAGAAGCTTGCGGTGGAGGCCTTCGAGGTGGCCATGGTCAGGACTGTGGCTCGGATTGTGCAGGGAGATGCAGGGAAGGTTGTCTGTAAAGAAGCAGAGAAGTTGAAGCCTGCTGCTGTTGTTATGGGCACCAGAGGAAGAAGCTTGATTCAAAGTGTTTTGCAGGGAAGTGTGAGTGAATATGTGTTCCACAACTGCAAATCAGCACCTGTTATAATAGTTCCTGGGAAAGGTACTATTTTG

Protein sequence

METLMEDEEEYSWREVRLPSLIPVVPEPELERETAERRRGRDILVAVDHGPNSKHALDWALVHFCRLADTIHLVHAVSNVKNELVYEASQGLMEKLAVEAFEVAMVRTVARIVQGDAGKVVCKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSEYVFHNCKSAPVIIVPGKGTIL

Homology

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BLAST of MS020539 vs. NCBI nr
Match: XP_022146303.1 (uncharacterized protein LOC111015542 isoform X1 [Momordica charantia])

HSP 1 Score: 335.1 bits (858), Expect = 3.6e-88
Identity = 170/170 (100.00%), Postives = 170/170 (100.00%), Query Frame = 0


Query: 1   METLMEDEEEYSWREVRLPSLIPVVPEPELERETAERRRGRDILVAVDHGPNSKHALDWA 60
           METLMEDEEEYSWREVRLPSLIPVVPEPELERETAERRRGRDILVAVDHGPNSKHALDWA
Sbjct: 1   METLMEDEEEYSWREVRLPSLIPVVPEPELERETAERRRGRDILVAVDHGPNSKHALDWA 60

Query: 61  LVHFCRLADTIHLVHAVSNVKNELVYEASQGLMEKLAVEAFEVAMVRTVARIVQGDAGKV 120
           LVHFCRLADTIHLVHAVSNVKNELVYEASQGLMEKLAVEAFEVAMVRTVARIVQGDAGKV
Sbjct: 61  LVHFCRLADTIHLVHAVSNVKNELVYEASQGLMEKLAVEAFEVAMVRTVARIVQGDAGKV 120

Query: 121 VCKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSEYVFHNCKSAPVIIVPGK 171
           VCKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSEYVFHNCKSAPVIIVPGK
Sbjct: 121 VCKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSEYVFHNCKSAPVIIVPGK 170

[Close]

BLAST of MS020539 vs. NCBI nr
Match: XP_038901616.1 (uncharacterized protein LOC120088411 [Benincasa hispida])

HSP 1 Score: 317.8 bits (813), Expect = 5.9e-83
Identity = 160/170 (94.12%), Postives = 167/170 (98.24%), Query Frame = 0


Query: 1   METLMEDEEEYSWREVRLPSLIPVVPEPELERETAERRRGRDILVAVDHGPNSKHALDWA 60
           M+TL E+EEEY+WREVRLPSLIPVVPEPELERETAERRRGRDIL+AVDHGPNSKHA DWA
Sbjct: 1   MDTL-EEEEEYNWREVRLPSLIPVVPEPELERETAERRRGRDILIAVDHGPNSKHAFDWA 60

Query: 61  LVHFCRLADTIHLVHAVSNVKNELVYEASQGLMEKLAVEAFEVAMVRTVARIVQGDAGKV 120
           L+HFCRLADTIHLVHAVSNVKNELVYE SQGLMEKLAVEAFEVAMVRTVARIVQGDAGKV
Sbjct: 61  LIHFCRLADTIHLVHAVSNVKNELVYEFSQGLMEKLAVEAFEVAMVRTVARIVQGDAGKV 120

Query: 121 VCKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSEYVFHNCKSAPVIIVPGK 171
           +CKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSE+VFHNCKSAPVIIVPGK
Sbjct: 121 ICKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSEHVFHNCKSAPVIIVPGK 169

[Close]

BLAST of MS020539 vs. NCBI nr
Match: KAG7010929.1 (hypothetical protein SDJN02_27727 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 317.8 bits (813), Expect = 5.9e-83
Identity = 160/171 (93.57%), Postives = 167/171 (97.66%), Query Frame = 0


Query: 1   METLMEDEEEYSWREVRLPSLIPVVPEPELERETAERRRGRDILVAVDHGPNSKHALDWA 60
           M+TL E+EEEY+WREVRLPSLIPVVPEPELERETA RRRGRDILVAVDHGPNSKHA DWA
Sbjct: 1   MDTL-EEEEEYNWREVRLPSLIPVVPEPELERETAVRRRGRDILVAVDHGPNSKHAFDWA 60

Query: 61  LVHFCRLADTIHLVHAVSNVKNELVYEASQGLMEKLAVEAFEVAMVRTVARIVQGDAGKV 120
           L+HFCRLADTIHLVHAVSNVKNELVYE SQGLMEKLAVEAFEVAMVRTVARIVQGDAGK+
Sbjct: 61  LIHFCRLADTIHLVHAVSNVKNELVYEYSQGLMEKLAVEAFEVAMVRTVARIVQGDAGKI 120

Query: 121 VCKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSEYVFHNCKSAPVIIVPGKG 172
           +CKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSE+VFHNCKSAPVIIVPGKG
Sbjct: 121 ICKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSEHVFHNCKSAPVIIVPGKG 170

[Close]

BLAST of MS020539 vs. NCBI nr
Match: XP_031737219.1 (uncharacterized protein LOC101210790 isoform X2 [Cucumis sativus])

HSP 1 Score: 317.4 bits (812), Expect = 7.7e-83
Identity = 159/170 (93.53%), Postives = 167/170 (98.24%), Query Frame = 0


Query: 1   METLMEDEEEYSWREVRLPSLIPVVPEPELERETAERRRGRDILVAVDHGPNSKHALDWA 60
           M+TL E+EEEY+WREVRLPSLIPVVPEPELERETAERRRGRDIL+AVDHGPNSKHA DWA
Sbjct: 1   MDTL-EEEEEYNWREVRLPSLIPVVPEPELERETAERRRGRDILIAVDHGPNSKHAFDWA 60

Query: 61  LVHFCRLADTIHLVHAVSNVKNELVYEASQGLMEKLAVEAFEVAMVRTVARIVQGDAGKV 120
           L+HFCRLADTIHLVHAVSNVKNELVYE SQGLMEKLAVEAFEVAMVRTVARIVQGDAGKV
Sbjct: 61  LIHFCRLADTIHLVHAVSNVKNELVYEFSQGLMEKLAVEAFEVAMVRTVARIVQGDAGKV 120

Query: 121 VCKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSEYVFHNCKSAPVIIVPGK 171
           +CKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSE+VFHNCKSAPV+IVPGK
Sbjct: 121 ICKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSEHVFHNCKSAPVVIVPGK 169

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BLAST of MS020539 vs. NCBI nr
Match: XP_004148842.1 (uncharacterized protein LOC101210790 isoform X1 [Cucumis sativus] >XP_008460941.1 PREDICTED: uncharacterized protein LOC103499676 [Cucumis melo] >XP_031737218.1 uncharacterized protein LOC101210790 isoform X1 [Cucumis sativus] >KGN61799.1 hypothetical protein Csa_005904 [Cucumis sativus] >TYK28846.1 universal stress protein PHOS32 [Cucumis melo var. makuwa])

HSP 1 Score: 317.4 bits (812), Expect = 7.7e-83
Identity = 159/170 (93.53%), Postives = 167/170 (98.24%), Query Frame = 0


Query: 1   METLMEDEEEYSWREVRLPSLIPVVPEPELERETAERRRGRDILVAVDHGPNSKHALDWA 60
           M+TL E+EEEY+WREVRLPSLIPVVPEPELERETAERRRGRDIL+AVDHGPNSKHA DWA
Sbjct: 1   MDTL-EEEEEYNWREVRLPSLIPVVPEPELERETAERRRGRDILIAVDHGPNSKHAFDWA 60

Query: 61  LVHFCRLADTIHLVHAVSNVKNELVYEASQGLMEKLAVEAFEVAMVRTVARIVQGDAGKV 120
           L+HFCRLADTIHLVHAVSNVKNELVYE SQGLMEKLAVEAFEVAMVRTVARIVQGDAGKV
Sbjct: 61  LIHFCRLADTIHLVHAVSNVKNELVYEFSQGLMEKLAVEAFEVAMVRTVARIVQGDAGKV 120

Query: 121 VCKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSEYVFHNCKSAPVIIVPGK 171
           +CKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSE+VFHNCKSAPV+IVPGK
Sbjct: 121 ICKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSEHVFHNCKSAPVVIVPGK 169

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BLAST of MS020539 vs. ExPASy Swiss-Prot
Match: P87132 (Uncharacterized protein C167.05 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC167.05 PE=1 SV=2)

HSP 1 Score: 47.0 bits (110), Expect = 2.6e-04
Identity = 37/156 (23.72%), Postives = 73/156 (46.79%), Query Frame = 0


Query: 29  ELERETAERRRGRDILVAVDHGPNSKHALDWALVHFCRLADTIHLVHAV----------- 88
           + +   +  +R     + +D    S HA +WA+    R  DT+ +V  +           
Sbjct: 419 DFQTAASSSKRNCTYFLTLDLSSESLHAAEWAVGILLRNGDTLIIVDVIECDDPSARAVK 478

Query: 89  ---SNVKNELVYEASQGLMEKLAVEAFEVAMVRTVARIVQGDAGKVVCKEAEKLKPAAVV 148
               + + E + + ++ +++ L+    EV +   +  I    A  ++ +  + ++P+ VV
Sbjct: 479 DRMESEQLETLEKITKYILKLLSKTVLEVEV--NIEVIHHEKAKHLIIEMIDYIEPSLVV 538

Query: 149 MGTRGRSLIQSVLQGSVSEYVFHNCKSAPVIIVPGK 171
           MG+RGRS ++ VL GS S Y+  N  S PV++   K
Sbjct: 539 MGSRGRSHLKGVLLGSFSNYLV-NKSSVPVMVARKK 571

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BLAST of MS020539 vs. ExPASy Swiss-Prot
Match: Q8VYN9 (Universal stress protein PHOS32 OS=Arabidopsis thaliana OX=3702 GN=PHOS32 PE=1 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 7.4e-04
Identity = 42/156 (26.92%), Postives = 67/156 (42.95%), Query Frame = 0


Query: 41  RDILVAVDHGPNSKHALDWALVHFCRLADTIHLVHAVSN------------VKNELVYEA 100
           R I VAVD    S  A+ WA+ H+ R  D + L+H                +K ++    
Sbjct: 48  RKIGVAVDLSEESSFAVRWAVDHYIRPGDAVVLLHVSPTSVLFGADWGPLPLKTQIEDPN 107

Query: 101 SQGLMEKLAVEAFEVAMVRTVAR-------------IVQGDAGKVVCKEAEKLKPAAVVM 160
           +Q    +   +AF    V  +A+             +   D  + +C E E+L  +AV+M
Sbjct: 108 AQPQPSQEDFDAFTSTKVADLAKPLKELGFPYKIHIVKDHDMRERLCLEIERLGLSAVIM 167

Query: 161 GTRG----RSLIQSVLQGSVSEYVFHNCKSAPVIIV 168
           G+RG    +        GSVS+Y  H+C   PV++V
Sbjct: 168 GSRGFGAEKKRGSDGKLGSVSDYCVHHC-VCPVVVV 202

[Close]

BLAST of MS020539 vs. ExPASy TrEMBL
Match: A0A6J1CY89 (uncharacterized protein LOC111015542 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111015542 PE=4 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 1.7e-88
Identity = 170/170 (100.00%), Postives = 170/170 (100.00%), Query Frame = 0


Query: 1   METLMEDEEEYSWREVRLPSLIPVVPEPELERETAERRRGRDILVAVDHGPNSKHALDWA 60
           METLMEDEEEYSWREVRLPSLIPVVPEPELERETAERRRGRDILVAVDHGPNSKHALDWA
Sbjct: 1   METLMEDEEEYSWREVRLPSLIPVVPEPELERETAERRRGRDILVAVDHGPNSKHALDWA 60

Query: 61  LVHFCRLADTIHLVHAVSNVKNELVYEASQGLMEKLAVEAFEVAMVRTVARIVQGDAGKV 120
           LVHFCRLADTIHLVHAVSNVKNELVYEASQGLMEKLAVEAFEVAMVRTVARIVQGDAGKV
Sbjct: 61  LVHFCRLADTIHLVHAVSNVKNELVYEASQGLMEKLAVEAFEVAMVRTVARIVQGDAGKV 120

Query: 121 VCKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSEYVFHNCKSAPVIIVPGK 171
           VCKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSEYVFHNCKSAPVIIVPGK
Sbjct: 121 VCKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSEYVFHNCKSAPVIIVPGK 170

[Close]

BLAST of MS020539 vs. ExPASy TrEMBL
Match: A0A5D3DYQ0 (Universal stress protein PHOS32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold303G00530 PE=4 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 3.7e-83
Identity = 159/170 (93.53%), Postives = 167/170 (98.24%), Query Frame = 0


Query: 1   METLMEDEEEYSWREVRLPSLIPVVPEPELERETAERRRGRDILVAVDHGPNSKHALDWA 60
           M+TL E+EEEY+WREVRLPSLIPVVPEPELERETAERRRGRDIL+AVDHGPNSKHA DWA
Sbjct: 1   MDTL-EEEEEYNWREVRLPSLIPVVPEPELERETAERRRGRDILIAVDHGPNSKHAFDWA 60

Query: 61  LVHFCRLADTIHLVHAVSNVKNELVYEASQGLMEKLAVEAFEVAMVRTVARIVQGDAGKV 120
           L+HFCRLADTIHLVHAVSNVKNELVYE SQGLMEKLAVEAFEVAMVRTVARIVQGDAGKV
Sbjct: 61  LIHFCRLADTIHLVHAVSNVKNELVYEFSQGLMEKLAVEAFEVAMVRTVARIVQGDAGKV 120

Query: 121 VCKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSEYVFHNCKSAPVIIVPGK 171
           +CKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSE+VFHNCKSAPV+IVPGK
Sbjct: 121 ICKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSEHVFHNCKSAPVVIVPGK 169

[Close]

BLAST of MS020539 vs. ExPASy TrEMBL
Match: A0A0A0LL61 (Usp domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G247600 PE=4 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 3.7e-83
Identity = 159/170 (93.53%), Postives = 167/170 (98.24%), Query Frame = 0


Query: 1   METLMEDEEEYSWREVRLPSLIPVVPEPELERETAERRRGRDILVAVDHGPNSKHALDWA 60
           M+TL E+EEEY+WREVRLPSLIPVVPEPELERETAERRRGRDIL+AVDHGPNSKHA DWA
Sbjct: 1   MDTL-EEEEEYNWREVRLPSLIPVVPEPELERETAERRRGRDILIAVDHGPNSKHAFDWA 60

Query: 61  LVHFCRLADTIHLVHAVSNVKNELVYEASQGLMEKLAVEAFEVAMVRTVARIVQGDAGKV 120
           L+HFCRLADTIHLVHAVSNVKNELVYE SQGLMEKLAVEAFEVAMVRTVARIVQGDAGKV
Sbjct: 61  LIHFCRLADTIHLVHAVSNVKNELVYEFSQGLMEKLAVEAFEVAMVRTVARIVQGDAGKV 120

Query: 121 VCKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSEYVFHNCKSAPVIIVPGK 171
           +CKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSE+VFHNCKSAPV+IVPGK
Sbjct: 121 ICKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSEHVFHNCKSAPVVIVPGK 169

[Close]

BLAST of MS020539 vs. ExPASy TrEMBL
Match: A0A1S3CET4 (uncharacterized protein LOC103499676 OS=Cucumis melo OX=3656 GN=LOC103499676 PE=4 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 3.7e-83
Identity = 159/170 (93.53%), Postives = 167/170 (98.24%), Query Frame = 0


Query: 1   METLMEDEEEYSWREVRLPSLIPVVPEPELERETAERRRGRDILVAVDHGPNSKHALDWA 60
           M+TL E+EEEY+WREVRLPSLIPVVPEPELERETAERRRGRDIL+AVDHGPNSKHA DWA
Sbjct: 1   MDTL-EEEEEYNWREVRLPSLIPVVPEPELERETAERRRGRDILIAVDHGPNSKHAFDWA 60

Query: 61  LVHFCRLADTIHLVHAVSNVKNELVYEASQGLMEKLAVEAFEVAMVRTVARIVQGDAGKV 120
           L+HFCRLADTIHLVHAVSNVKNELVYE SQGLMEKLAVEAFEVAMVRTVARIVQGDAGKV
Sbjct: 61  LIHFCRLADTIHLVHAVSNVKNELVYEFSQGLMEKLAVEAFEVAMVRTVARIVQGDAGKV 120

Query: 121 VCKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSEYVFHNCKSAPVIIVPGK 171
           +CKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSE+VFHNCKSAPV+IVPGK
Sbjct: 121 ICKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSEHVFHNCKSAPVVIVPGK 169

[Close]

BLAST of MS020539 vs. ExPASy TrEMBL
Match: A0A6J1FTC2 (uncharacterized protein LOC111448293 OS=Cucurbita moschata OX=3662 GN=LOC111448293 PE=4 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 1.9e-82
Identity = 159/170 (93.53%), Postives = 166/170 (97.65%), Query Frame = 0


Query: 1   METLMEDEEEYSWREVRLPSLIPVVPEPELERETAERRRGRDILVAVDHGPNSKHALDWA 60
           M+TL E+EEEY+WREVRLPSLIPVVPEPELERETA RRRGRDILVAVDHGPNSKHA DWA
Sbjct: 1   MDTL-EEEEEYNWREVRLPSLIPVVPEPELERETAVRRRGRDILVAVDHGPNSKHAFDWA 60

Query: 61  LVHFCRLADTIHLVHAVSNVKNELVYEASQGLMEKLAVEAFEVAMVRTVARIVQGDAGKV 120
           L+HFCRLADTIHLVHAVSNVKNELVYE SQGLMEKLAVEAFEVAMVRTVARIVQGDAGK+
Sbjct: 61  LIHFCRLADTIHLVHAVSNVKNELVYEYSQGLMEKLAVEAFEVAMVRTVARIVQGDAGKI 120

Query: 121 VCKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSEYVFHNCKSAPVIIVPGK 171
           +CKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSE+VFHNCKSAPVIIVPGK
Sbjct: 121 ICKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSEHVFHNCKSAPVIIVPGK 169

[Close]

BLAST of MS020539 vs. TAIR 10
Match: AT2G21620.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 288.9 bits (738), Expect = 2.7e-78
Identity = 141/170 (82.94%), Postives = 161/170 (94.71%), Query Frame = 0


Query: 1   METLMEDEEEYSWREVRLPSLIPVVPEPELERETAERRRGRDILVAVDHGPNSKHALDWA 60
           ME L ED EEYS+REV LPSLIPVVPEPELERE+ ERRRGRD++VAVDHGPNSKHA DWA
Sbjct: 1   MEALPED-EEYSFREVVLPSLIPVVPEPELERESGERRRGRDVIVAVDHGPNSKHAFDWA 60

Query: 61  LVHFCRLADTIHLVHAVSNVKNELVYEASQGLMEKLAVEAFEVAMVRTVARIVQGDAGKV 120
           LVHFCRLADT+HLVHAVS+VKN++VYE SQ LMEKLAVEA++VAMV++VAR+V+GDAGKV
Sbjct: 61  LVHFCRLADTLHLVHAVSSVKNDVVYETSQALMEKLAVEAYQVAMVKSVARVVEGDAGKV 120

Query: 121 VCKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSEYVFHNCKSAPVIIVPGK 171
           +CKEAEK+KPAAV++GTRGRSL++SVLQGSVSEY FHNCKSAPVIIVPGK
Sbjct: 121 ICKEAEKVKPAAVIVGTRGRSLVRSVLQGSVSEYCFHNCKSAPVIIVPGK 169

[Close]

BLAST of MS020539 vs. TAIR 10
Match: AT2G21620.2 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 282.3 bits (721), Expect = 2.6e-76
Identity = 141/176 (80.11%), Postives = 161/176 (91.48%), Query Frame = 0


Query: 1   METLMEDEEEYSWREVRLPSLIPVVPEPELERETAERRRGRDILVAVDHGPNSKHALDWA 60
           ME L ED EEYS+REV LPSLIPVVPEPELERE+ ERRRGRD++VAVDHGPNSKHA DWA
Sbjct: 1   MEALPED-EEYSFREVVLPSLIPVVPEPELERESGERRRGRDVIVAVDHGPNSKHAFDWA 60

Query: 61  LVHFCRLADTIHLVHAVSN------VKNELVYEASQGLMEKLAVEAFEVAMVRTVARIVQ 120
           LVHFCRLADT+HLVHAVS+      VKN++VYE SQ LMEKLAVEA++VAMV++VAR+V+
Sbjct: 61  LVHFCRLADTLHLVHAVSSSFSLQCVKNDVVYETSQALMEKLAVEAYQVAMVKSVARVVE 120

Query: 121 GDAGKVVCKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSEYVFHNCKSAPVIIVPGK 171
           GDAGKV+CKEAEK+KPAAV++GTRGRSL++SVLQGSVSEY FHNCKSAPVIIVPGK
Sbjct: 121 GDAGKVICKEAEKVKPAAVIVGTRGRSLVRSVLQGSVSEYCFHNCKSAPVIIVPGK 175

[Close]

BLAST of MS020539 vs. TAIR 10
Match: AT3G53990.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 60.5 bits (145), Expect = 1.6e-09
Identity = 43/155 (27.74%), Postives = 75/155 (48.39%), Query Frame = 0


Query: 39  RGRDILVAVDHGPNSKHALDWALVHFCRLADTIHLVHAV----SNVKNELVYEASQGL-- 98
           + R+I +A+D   +SK+AL WA+ +     DTI+++H +       +N L +++   L  
Sbjct: 3   KDRNIGIAMDFSESSKNALKWAIENLADKGDTIYIIHTLPLSGDESRNSLWFKSGSPLIP 62

Query: 99  ---------MEKLAVEAFEVAM-----------VRTVARIVQGDAGKVVCKEAEKLKPAA 158
                    MEK  V+     +           V  V ++  GDA + +    + LK  +
Sbjct: 63  LAEFREPEIMEKYGVKTDIACLDMLDTGSRQKEVHVVTKLYWGDAREKLVDAVKDLKLDS 122

Query: 159 VVMGTRGRSLIQSVLQGSVSEYVFHNCKSAPVIIV 168
           +VMG+RG S +Q ++ GSVS +V  +    PV +V
Sbjct: 123 IVMGSRGLSALQRIIMGSVSSFVIQHA-PCPVTVV 156

[Close]

BLAST of MS020539 vs. TAIR 10
Match: AT3G11930.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 57.0 bits (136), Expect = 1.8e-08
Identity = 44/184 (23.91%), Postives = 79/184 (42.93%), Query Frame = 0


Query: 20  SLIPVVPEPELERETAERRRGRDILVAVDHGPNSKHALDWALVHFCRLADT--------- 79
           S +   PE   E E       R ++VA+D   +S +AL W + HF  L  T         
Sbjct: 13  SAVEKQPETTTEAEAPSLTTKR-MVVAIDESDSSFYALQWVIDHFSNLLLTTAAAEAESG 72

Query: 80  -IHLVHAVSNVKNELVYEASQG---------LMEKL--------------AVEAFEVAMV 139
            + ++H  S   +   + A  G         ++E +              A++      +
Sbjct: 73  MLTVIHVQSPFNHFAAFPAGPGGATVYASSSMIESVKKAQQETSAALLSRALQMCRAKQI 132

Query: 140 RTVARIVQGDAGKVVCKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSEYVFHNCKSAPVII 171
           RT   +++G+A +++C+  EK+    +V+G+RG   I+    GSVS+Y  H+     +I+
Sbjct: 133 RTETLVLEGEAKEMICEAVEKMHVDLLVVGSRGLGKIKRAFLGSVSDYCAHHANCPILIV 192

[Close]

BLAST of MS020539 vs. TAIR 10
Match: AT3G11930.2 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 56.6 bits (135), Expect = 2.3e-08
Identity = 44/185 (23.78%), Postives = 79/185 (42.70%), Query Frame = 0


Query: 20  SLIPVVPEPELERETAERRRGRDILVAVDHGPNSKHALDWALVHFCRLADT--------- 79
           S +   PE   E E       R ++VA+D   +S +AL W + HF  L  T         
Sbjct: 13  SAVEKQPETTTEAEAPSLTTKR-MVVAIDESDSSFYALQWVIDHFSNLLLTTAAAEAESG 72

Query: 80  -IHLVHAVSNVKNELVYEASQG----------LMEKL--------------AVEAFEVAM 139
            + ++H  S   +   + A  G          ++E +              A++      
Sbjct: 73  MLTVIHVQSPFNHFAAFPAGPGGATAVYASSSMIESVKKAQQETSAALLSRALQMCRAKQ 132

Query: 140 VRTVARIVQGDAGKVVCKEAEKLKPAAVVMGTRGRSLIQSVLQGSVSEYVFHNCKSAPVI 171
           +RT   +++G+A +++C+  EK+    +V+G+RG   I+    GSVS+Y  H+     +I
Sbjct: 133 IRTETLVLEGEAKEMICEAVEKMHVDLLVVGSRGLGKIKRAFLGSVSDYCAHHANCPILI 192

The following BLAST results are available for this feature:

BLAST of MS020539 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146303.1	3.6e-88	100.00	uncharacterized protein LOC111015542 isoform X1 [Momordica charantia]	[more]
XP_038901616.1	5.9e-83	94.12	uncharacterized protein LOC120088411 [Benincasa hispida]	[more]
KAG7010929.1	5.9e-83	93.57	hypothetical protein SDJN02_27727 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_031737219.1	7.7e-83	93.53	uncharacterized protein LOC101210790 isoform X2 [Cucumis sativus]	[more]
XP_004148842.1	7.7e-83	93.53	uncharacterized protein LOC101210790 isoform X1 [Cucumis sativus] >XP_008460941....	[more]

BLAST of MS020539 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P87132	2.6e-04	23.72	Uncharacterized protein C167.05 OS=Schizosaccharomyces pombe (strain 972 / ATCC ...	[more]
Q8VYN9	7.4e-04	26.92	Universal stress protein PHOS32 OS=Arabidopsis thaliana OX=3702 GN=PHOS32 PE=1 S...	[more]

BLAST of MS020539 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CY89	1.7e-88	100.00	uncharacterized protein LOC111015542 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A5D3DYQ0	3.7e-83	93.53	Universal stress protein PHOS32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A0A0LL61	3.7e-83	93.53	Usp domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G247600 PE=4 SV...	[more]
A0A1S3CET4	3.7e-83	93.53	uncharacterized protein LOC103499676 OS=Cucumis melo OX=3656 GN=LOC103499676 PE=...	[more]
A0A6J1FTC2	1.9e-82	93.53	uncharacterized protein LOC111448293 OS=Cucurbita moschata OX=3662 GN=LOC1114482...	[more]

BLAST of MS020539 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G21620.1	2.7e-78	82.94	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT2G21620.2	2.6e-76	80.11	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT3G53990.1	1.6e-09	27.74	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT3G11930.1	1.8e-08	23.91	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT3G11930.2	2.3e-08	23.78	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006015	Universal stress protein A family	PRINTS	PR01438	UNVRSLSTRESS	coord: 40..58 score: 28.42 coord: 145..167 score: 26.78 coord: 127..139 score: 33.23
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 40..169 e-value: 1.1E-26 score: 96.0
IPR006016	UspA	PFAM	PF00582	Usp	coord: 41..168 e-value: 3.2E-20 score: 73.1
None	No IPR available	PANTHER	PTHR47583	ADENINE NUCLEOTIDE ALPHA HYDROLASES-LIKE SUPERFAMILY PROTEIN	coord: 1..171
None	No IPR available	CDD	cd00293	USP_Like	coord: 43..167 e-value: 1.07813E-25 score: 93.5886
None	No IPR available	SUPERFAMILY	52402	Adenine nucleotide alpha hydrolases-like	coord: 41..170

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS020539.1	MS020539.1	mRNA