MS020508 (gene) Bitter gourd (TR) v1

Overview
NameMS020508
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationscaffold375: 764951 .. 766159 (-)
RNA-Seq ExpressionMS020508
SyntenyMS020508
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGACCGCCGCCGTCCCCTCCCCGTCTCCTTCTCCTTCTCCTTCTCCTTCCTCCTCCGCCCTCAATTTCGTCCCGCCGTCGTCGCTTCAGCCGCCCAAGCTCCCCGTCGACTTCAGTCCTCCTCTAATTGCCATGGTTGTGGTCATCGCTACGGCCTTCTTGATCGTCACCTACTCCCGCCTCATCTCCCGCCACCTCATCCCTCCCATCCTCCACCTCCTCCGCCTTCACCACCGCCGTTGGCGCCGCTGGAGGCGCCGCCGCCGCCGTGCCAGATCTTATCTTCCGTCTTCCTCCGCCGGCGACCTGGATTCCCTCCCTTTCGACTCGCCTAATTTCTTCGAGCCTGCCGATGCCTTCCACGTGTTCTCTCCGTACGGACTCGATGAGTCTGTTATCAAGACTATTCCGCTCTCCGTCTATACTGCCAAGAGCCGCCGCGATAGCCGCGACCGTGAGTGCGCCGTTTGCCTTCTGGAGTTCGAGGACGACGACTACGTCCGCACGCTTCCGGTGTGCTCCCACGCGTTTCACGTTGACTGCATCGACGTTTGGCTTAGGTCTCACGCAAACTGTCCGCTCTGTCGCGCGGTGGTTTTCCGGCCGGAATCTCCGTTTACTCCCGTGATGGCGGCGAGAATCCGGCCGAGCCTCGACGATACGATCTTACGGAGCATCGTATTGGAGCCGCTCGCGGGAGCTACTAGGGATTCTAACGCAACGGTGTCGGAGATCACGCCGTGCGTCGACGAACCGCGGAATAGCAACTCCGAGGATCAAATCAACGGCCGAGACTTCTTGCTGAAGCGATCGTATTCGTTCGGATTCGAACGGAGCTTGGCGTCGGAACGAATGATCCTGGAGCCGGCAACGGCGTCGCCTTGGAGGTACCGCCGCGGAAGCTTCTGGAGCAAACGGCCGTCTCCTTTCGGATCTCTGCCGAAGGCGAGAGTATTCTCCTTCCGATACTACAGAGGAATGAAGTCGCCGTTCTTCAGACGCCGAGGATTCTTCCCGCTATCGGAATCAAGCTGGAGATTCTCAAACGGCGCAGGCGGCGGTTCGTCGAGACGAACCAAATCGATGACGAGTCCGATGTTTCTCCGATCATCGGCAACCGGCGGAGGAGCCGCCGCGTTCTCGTCGAGCCGGTTGAGATGCGGAGATCCCGAGGCGTTACTGTCACCGGAAAGATTCAACCGAAGA

mRNA sequence

ATGCCGACCGCCGCCGTCCCCTCCCCGTCTCCTTCTCCTTCTCCTTCTCCTTCCTCCTCCGCCCTCAATTTCGTCCCGCCGTCGTCGCTTCAGCCGCCCAAGCTCCCCGTCGACTTCAGTCCTCCTCTAATTGCCATGGTTGTGGTCATCGCTACGGCCTTCTTGATCGTCACCTACTCCCGCCTCATCTCCCGCCACCTCATCCCTCCCATCCTCCACCTCCTCCGCCTTCACCACCGCCGTTGGCGCCGCTGGAGGCGCCGCCGCCGCCGTGCCAGATCTTATCTTCCGTCTTCCTCCGCCGGCGACCTGGATTCCCTCCCTTTCGACTCGCCTAATTTCTTCGAGCCTGCCGATGCCTTCCACGTGTTCTCTCCGTACGGACTCGATGAGTCTGTTATCAAGACTATTCCGCTCTCCGTCTATACTGCCAAGAGCCGCCGCGATAGCCGCGACCGTGAGTGCGCCGTTTGCCTTCTGGAGTTCGAGGACGACGACTACGTCCGCACGCTTCCGGTGTGCTCCCACGCGTTTCACGTTGACTGCATCGACGTTTGGCTTAGGTCTCACGCAAACTGTCCGCTCTGTCGCGCGGTGGTTTTCCGGCCGGAATCTCCGTTTACTCCCGTGATGGCGGCGAGAATCCGGCCGAGCCTCGACGATACGATCTTACGGAGCATCGTATTGGAGCCGCTCGCGGGAGCTACTAGGGATTCTAACGCAACGGTGTCGGAGATCACGCCGTGCGTCGACGAACCGCGGAATAGCAACTCCGAGGATCAAATCAACGGCCGAGACTTCTTGCTGAAGCGATCGTATTCGTTCGGATTCGAACGGAGCTTGGCGTCGGAACGAATGATCCTGGAGCCGGCAACGGCGTCGCCTTGGAGGTACCGCCGCGGAAGCTTCTGGAGCAAACGGCCGTCTCCTTTCGGATCTCTGCCGAAGGCGAGAGTATTCTCCTTCCGATACTACAGAGGAATGAAGTCGCCGTTCTTCAGACGCCGAGGATTCTTCCCGCTATCGGAATCAAGCTGGAGATTCTCAAACGGCGCAGGCGGCGGTTCGTCGAGACGAACCAAATCGATGACGAGTCCGATGTTTCTCCGATCATCGGCAACCGGCGGAGGAGCCGCCGCGTTCTCGTCGAGCCGGTTGAGATGCGGAGATCCCGAGGCGTTACTGTCACCGGAAAGATTCAACCGAAGA

Coding sequence (CDS)

ATGCCGACCGCCGCCGTCCCCTCCCCGTCTCCTTCTCCTTCTCCTTCTCCTTCCTCCTCCGCCCTCAATTTCGTCCCGCCGTCGTCGCTTCAGCCGCCCAAGCTCCCCGTCGACTTCAGTCCTCCTCTAATTGCCATGGTTGTGGTCATCGCTACGGCCTTCTTGATCGTCACCTACTCCCGCCTCATCTCCCGCCACCTCATCCCTCCCATCCTCCACCTCCTCCGCCTTCACCACCGCCGTTGGCGCCGCTGGAGGCGCCGCCGCCGCCGTGCCAGATCTTATCTTCCGTCTTCCTCCGCCGGCGACCTGGATTCCCTCCCTTTCGACTCGCCTAATTTCTTCGAGCCTGCCGATGCCTTCCACGTGTTCTCTCCGTACGGACTCGATGAGTCTGTTATCAAGACTATTCCGCTCTCCGTCTATACTGCCAAGAGCCGCCGCGATAGCCGCGACCGTGAGTGCGCCGTTTGCCTTCTGGAGTTCGAGGACGACGACTACGTCCGCACGCTTCCGGTGTGCTCCCACGCGTTTCACGTTGACTGCATCGACGTTTGGCTTAGGTCTCACGCAAACTGTCCGCTCTGTCGCGCGGTGGTTTTCCGGCCGGAATCTCCGTTTACTCCCGTGATGGCGGCGAGAATCCGGCCGAGCCTCGACGATACGATCTTACGGAGCATCGTATTGGAGCCGCTCGCGGGAGCTACTAGGGATTCTAACGCAACGGTGTCGGAGATCACGCCGTGCGTCGACGAACCGCGGAATAGCAACTCCGAGGATCAAATCAACGGCCGAGACTTCTTGCTGAAGCGATCGTATTCGTTCGGATTCGAACGGAGCTTGGCGTCGGAACGAATGATCCTGGAGCCGGCAACGGCGTCGCCTTGGAGGTACCGCCGCGGAAGCTTCTGGAGCAAACGGCCGTCTCCTTTCGGATCTCTGCCGAAGGCGAGAGTATTCTCCTTCCGATACTACAGAGGAATGAAGTCGCCGTTCTTCAGACGCCGAGGATTCTTCCCGCTATCGGAATCAAGCTGGAGATTCTCAAACGGCGCAGGCGGCGGTTCGTCGAGACGAACCAAATCGATGACGAGTCCGATGTTTCTCCGATCATCGGCAACCGGCGGAGGAGCCGCCGCGTTCTCGTCGAGCCGGTTGAGATGCGGAGATCCCGAGGCGTTACTGTCACCGGAAAGATTCAACCGAAGA

Protein sequence

MPTAAVPSPSPSPSPSPSSSALNFVPPSSLQPPKLPVDFSPPLIAMVVVIATAFLIVTYSRLISRHLIPPILHLLRLHHRRWRRWRRRRRRARSYLPSSSAGDLDSLPFDSPNFFEPADAFHVFSPYGLDESVIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPVCSHAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTILRSIVLEPLAGATRDSNATVSEITPCVDEPRNSNSEDQINGRDFLLKRSYSFGFERSLASERMILEPATASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGFFPLSESSWRFSNGAGGGSSRRTKSMTSPMFLRSSATGGGAAAFSSSRLRCGDPEALLSPERFNRR
Homology
BLAST of MS020508 vs. NCBI nr
Match: XP_038902895.1 (RING-H2 finger protein ATL65 [Benincasa hispida])

HSP 1 Score: 698.7 bits (1802), Expect = 2.9e-197
Identity = 372/409 (90.95%), Postives = 379/409 (92.67%), Query Frame = 0

Query: 1   MPTAAVPSPSPSPSPSPSSSALNFVPPSSLQPPKLPVDFSPPLIAMVVVIATAFLIVTYS 60
           MPTA  PSPSPSPSPS ++SA NF PPSSLQP KLPVDFSPPLIAMVVVIATAFLIVTYS
Sbjct: 1   MPTA--PSPSPSPSPSTAASAANFPPPSSLQPAKLPVDFSPPLIAMVVVIATAFLIVTYS 60

Query: 61  RLISRHLIPPILHLLRLHHRRWRRWRRRRRRARSYLPSSSAGDLDSLPFDSPNFFEPADA 120
           RLISRHLIPPILHLLRLH RRWRRWRRRRRRARSYLPSSS GDLDSLP+DSP FFEP D 
Sbjct: 61  RLISRHLIPPILHLLRLHRRRWRRWRRRRRRARSYLPSSSGGDLDSLPYDSP-FFEPTDG 120

Query: 121 FHVFSPYGLDESVIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPVCSHAFHV 180
           FHVFSPYGLDESVIKTIPLSVYTAK    SRDRECAVCLLEFEDDDYVRTLP+CSHAFHV
Sbjct: 121 FHVFSPYGLDESVIKTIPLSVYTAK----SRDRECAVCLLEFEDDDYVRTLPICSHAFHV 180

Query: 181 DCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTILRSIVLEPLAGAT---R 240
           DCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTILRSIVLEPL   T   R
Sbjct: 181 DCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTILRSIVLEPLVEGTIPNR 240

Query: 241 DS-NATVSEITPCVDEPRNSNS-EDQINGRDFLLKRSYSFGFERSLASERMILEPATASP 300
           DS +AT SEITPCVDEPRNSNS +DQINGRDFLLKRSYSFGFERSLASERMILEPAT SP
Sbjct: 241 DSYHATESEITPCVDEPRNSNSVDDQINGRDFLLKRSYSFGFERSLASERMILEPATTSP 300

Query: 301 WRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRR-GFFPLSESSWRFSNGAGG 360
           WRYRRGSFW KRPSPFGSLPKARVFSFRYYRGMKSPFFRRR GFFPLSESSWRFSNG GG
Sbjct: 301 WRYRRGSFWMKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFSNGVGG 360

Query: 361 GSSRRTKSMTSPMFLRSSATGGGAAAFSSSRLRCGDPEALLSPERFNRR 404
           GSSRR+KSMTSPMFLRSS TGGGAA FSSSRLRCGDPEALLSPERFNRR
Sbjct: 361 GSSRRSKSMTSPMFLRSSVTGGGAATFSSSRLRCGDPEALLSPERFNRR 402

BLAST of MS020508 vs. NCBI nr
Match: XP_022986546.1 (RING-H2 finger protein ATL65 [Cucurbita maxima])

HSP 1 Score: 691.8 bits (1784), Expect = 3.5e-195
Identity = 370/414 (89.37%), Postives = 379/414 (91.55%), Query Frame = 0

Query: 1   MPTAAVPSPSPSPSPSPSSS-----ALNFVPPSSLQPPKLPVDFSPPLIAMVVVIATAFL 60
           M TA  PSPSPSPSPS SSS     A+NF PPSSL P KLPVDFSPPLIAMVVVIATAFL
Sbjct: 1   MSTAPSPSPSPSPSPSTSSSSSSAAAVNFPPPSSLLPAKLPVDFSPPLIAMVVVIATAFL 60

Query: 61  IVTYSRLISRHLIPPILHLLRLHHRRWRRWRRRRRRARSYLPSSSAGDLDSLPFDSPNFF 120
           IVTYSRLISRH+IPPIL L+R H RRWRRWRRRRRRARSYLPSSS GDLDSLP+DSP FF
Sbjct: 61  IVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLPSSSGGDLDSLPYDSP-FF 120

Query: 121 EPADAFHVFSPYGLDESVIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPVCS 180
           E  D FHVFSPYGLDES+IKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLP+CS
Sbjct: 121 EHTDGFHVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPICS 180

Query: 181 HAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTILRSIVLEPLAGA 240
           HAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTIL+SIVLEPLA  
Sbjct: 181 HAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTILQSIVLEPLAEG 240

Query: 241 ---TRDS-NATVSEITPCVDEPRNSNS-EDQINGRDFLLKRSYSFGFERSLASERMILEP 300
               RDS NAT SEITP VDEPRNSNS EDQ+NGRDFLLKRSYSFGFERSLASERMILEP
Sbjct: 241 PIPNRDSYNATESEITPYVDEPRNSNSLEDQMNGRDFLLKRSYSFGFERSLASERMILEP 300

Query: 301 ATASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRR-GFFPLSESSWRFS 360
           ATASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRR GFFPLSESSWRFS
Sbjct: 301 ATASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFS 360

Query: 361 NGAGGGSSRRTKSMTSPMFLRSSATGGGAAAFSSSRLRCGDPEALLSPERFNRR 404
           NG GGGSSRR+KSMTSPMFLRSS T GGAA FSSSRLRCGDPEALLSPERFNRR
Sbjct: 361 NGVGGGSSRRSKSMTSPMFLRSSVTSGGAATFSSSRLRCGDPEALLSPERFNRR 413

BLAST of MS020508 vs. NCBI nr
Match: XP_022943929.1 (RING-H2 finger protein ATL65 [Cucurbita moschata] >KAG7010952.1 RING-H2 finger protein ATL65, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 691.0 bits (1782), Expect = 6.0e-195
Identity = 368/414 (88.89%), Postives = 380/414 (91.79%), Query Frame = 0

Query: 1   MPTAAVPSPSPSPSPSPSSS-----ALNFVPPSSLQPPKLPVDFSPPLIAMVVVIATAFL 60
           M TA  PSPSPSPSPSPS+S     A+NF  PSSL P KLPVDFSPPLIAMVVVIATAFL
Sbjct: 1   MSTAPSPSPSPSPSPSPSTSSSAAAAVNFPSPSSLLPGKLPVDFSPPLIAMVVVIATAFL 60

Query: 61  IVTYSRLISRHLIPPILHLLRLHHRRWRRWRRRRRRARSYLPSSSAGDLDSLPFDSPNFF 120
           IVTYSRLISRH+IPPIL L+R H RRWRRWRRRRRRARSYLPSSS GDLDSLP+DSP FF
Sbjct: 61  IVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLPSSSGGDLDSLPYDSP-FF 120

Query: 121 EPADAFHVFSPYGLDESVIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPVCS 180
           E  D FHVFSPYGLDES+IKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLP+CS
Sbjct: 121 EHTDGFHVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPICS 180

Query: 181 HAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTILRSIVLEPLAGA 240
           HAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLD+TIL+SIVLEPLA  
Sbjct: 181 HAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDETILQSIVLEPLAEG 240

Query: 241 ---TRDS-NATVSEITPCVDEPRNSNS-EDQINGRDFLLKRSYSFGFERSLASERMILEP 300
               RDS +AT SEITPCVDEPRNSNS EDQ+NGRDFLLKRSYSFGFERSLASERMILEP
Sbjct: 241 PIPNRDSYHATESEITPCVDEPRNSNSLEDQMNGRDFLLKRSYSFGFERSLASERMILEP 300

Query: 301 ATASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRR-GFFPLSESSWRFS 360
           ATASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRR GFFPLSESSWRFS
Sbjct: 301 ATASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFS 360

Query: 361 NGAGGGSSRRTKSMTSPMFLRSSATGGGAAAFSSSRLRCGDPEALLSPERFNRR 404
           NG GGGSSRR+KSMTSPMFLRSS T GGAA FSSSRLRCGDPEALLSPERFNRR
Sbjct: 361 NGVGGGSSRRSKSMTSPMFLRSSVTSGGAATFSSSRLRCGDPEALLSPERFNRR 413

BLAST of MS020508 vs. NCBI nr
Match: XP_023511773.1 (RING-H2 finger protein ATL65 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 691.0 bits (1782), Expect = 6.0e-195
Identity = 368/415 (88.67%), Postives = 380/415 (91.57%), Query Frame = 0

Query: 1   MPTAAVPSPSPSPSPSPSSS------ALNFVPPSSLQPPKLPVDFSPPLIAMVVVIATAF 60
           M TA  PSPS SPSPSPS+S      A+NF PPSSL P KLPVDFSPPLIAMVVVIATAF
Sbjct: 1   MSTAPSPSPSSSPSPSPSTSSSSYAAAVNFPPPSSLLPGKLPVDFSPPLIAMVVVIATAF 60

Query: 61  LIVTYSRLISRHLIPPILHLLRLHHRRWRRWRRRRRRARSYLPSSSAGDLDSLPFDSPNF 120
           LIVTYSRLISRH+IPPIL L+R H RRWRRWRRRRRRARSYLPSSS GDLDSLP+DSP F
Sbjct: 61  LIVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLPSSSGGDLDSLPYDSP-F 120

Query: 121 FEPADAFHVFSPYGLDESVIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPVC 180
           FE  D +HVFSPYGLDES+IKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLP+C
Sbjct: 121 FEHTDGYHVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPIC 180

Query: 181 SHAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTILRSIVLEPLAG 240
           SHAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTIL+SIVLEPLA 
Sbjct: 181 SHAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTILQSIVLEPLAE 240

Query: 241 A---TRDS-NATVSEITPCVDEPRNSNS-EDQINGRDFLLKRSYSFGFERSLASERMILE 300
                RDS +AT SEITPCVDEPRNSNS EDQ+NGRDFLLKRSYSFGFERSLASERMILE
Sbjct: 241 GPIPNRDSYHATESEITPCVDEPRNSNSLEDQMNGRDFLLKRSYSFGFERSLASERMILE 300

Query: 301 PATASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRR-GFFPLSESSWRF 360
           PATASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRR GFFPLSESSWRF
Sbjct: 301 PATASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRF 360

Query: 361 SNGAGGGSSRRTKSMTSPMFLRSSATGGGAAAFSSSRLRCGDPEALLSPERFNRR 404
           SNG GGGSSRR+KSMTSPMFLRSS T GGAA FSSSRLRCGDPEALLSPERFNRR
Sbjct: 361 SNGVGGGSSRRSKSMTSPMFLRSSVTSGGAATFSSSRLRCGDPEALLSPERFNRR 414

BLAST of MS020508 vs. NCBI nr
Match: KAG6571140.1 (RING-H2 finger protein ATL65, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 689.9 bits (1779), Expect = 1.3e-194
Identity = 367/414 (88.65%), Postives = 380/414 (91.79%), Query Frame = 0

Query: 1   MPTAAVPSPSPSPSPSPSSSA-----LNFVPPSSLQPPKLPVDFSPPLIAMVVVIATAFL 60
           M TA  PSPSPSPSPSPS+S+     +NF  PSSL P KLPVDFSPPLIAMVVVIATAFL
Sbjct: 1   MSTAPSPSPSPSPSPSPSTSSSTAAPVNFPSPSSLLPGKLPVDFSPPLIAMVVVIATAFL 60

Query: 61  IVTYSRLISRHLIPPILHLLRLHHRRWRRWRRRRRRARSYLPSSSAGDLDSLPFDSPNFF 120
           IVTYSRLISRH+IPPIL L+R H RRWRRWRRRRRRARSYLPSSS GDLDSLP+DSP FF
Sbjct: 61  IVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLPSSSGGDLDSLPYDSP-FF 120

Query: 121 EPADAFHVFSPYGLDESVIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPVCS 180
           E  D FHVFSPYGLDES+IKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLP+CS
Sbjct: 121 EHTDGFHVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPICS 180

Query: 181 HAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTILRSIVLEPLAGA 240
           HAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLD+TIL+SIVLEPLA  
Sbjct: 181 HAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDETILQSIVLEPLAEG 240

Query: 241 ---TRDS-NATVSEITPCVDEPRNSNS-EDQINGRDFLLKRSYSFGFERSLASERMILEP 300
               RDS +AT SEITPCVDEPRNSNS EDQ+NGRDFLLKRSYSFGFERSLASERMILEP
Sbjct: 241 PIPNRDSYHATESEITPCVDEPRNSNSLEDQMNGRDFLLKRSYSFGFERSLASERMILEP 300

Query: 301 ATASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRR-GFFPLSESSWRFS 360
           ATASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRR GFFPLSESSWRFS
Sbjct: 301 ATASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFS 360

Query: 361 NGAGGGSSRRTKSMTSPMFLRSSATGGGAAAFSSSRLRCGDPEALLSPERFNRR 404
           NG GGGSSRR+KSMTSPMFLRSS T GGAA FSSSRLRCGDPEALLSPERFNRR
Sbjct: 361 NGVGGGSSRRSKSMTSPMFLRSSVTSGGAATFSSSRLRCGDPEALLSPERFNRR 413

BLAST of MS020508 vs. ExPASy Swiss-Prot
Match: Q67YI6 (RING-H2 finger protein ATL65 OS=Arabidopsis thaliana OX=3702 GN=ATL65 PE=2 SV=2)

HSP 1 Score: 341.3 bits (874), Expect = 1.5e-92
Identity = 224/416 (53.85%), Postives = 265/416 (63.70%), Query Frame = 0

Query: 12  SPSPSPSSSALNFVPPSSLQPPKLPVDFSPPLIAMVVVIATAFLIVTYSRLISRHLIPPI 71
           SPSPSPSS     +   S  P   P++FSPPLIAMVVV+A AFL VTYSRLISR  + P+
Sbjct: 14  SPSPSPSSGISEEILSRSSDP---PLEFSPPLIAMVVVLAAAFLFVTYSRLISRRFLSPL 73

Query: 72  LHLLRLHHRRWRRWRRRRRRARSYLPSSSAGDLDSLPFDSPNFFEPADAFHV--FSPYGL 131
                   RR+RRWR RRRR   +L S+S+    S    S + F P D+FH   +SPYGL
Sbjct: 74  F-------RRFRRWRCRRRRL-LHLSSASSASTSSSDLRSFSPF-PFDSFHYSSYSPYGL 133

Query: 132 DESVIKTIPLSVYTAKS---------RRDSRDRECAVCLLEFEDDDYVRTLPVCSHAFHV 191
           D+SVIKT+PL +Y+A +            +  R+CAVCLLEFE+ DYVRTLP+C HAFH+
Sbjct: 134 DDSVIKTLPLFLYSAAACTGKPAVGKTSAANCRDCAVCLLEFEEGDYVRTLPLCFHAFHL 193

Query: 192 DCIDVWLRSHANCPLCR------AVVFRPESPFTPVMAARIRPSLDDTILRSIVLEPLAG 251
           +CID WLRSH NCPLCR      A V  P SPF P+MA RIRPSLDD    +I++     
Sbjct: 194 ECIDEWLRSHPNCPLCRTAILGSAGVLTPMSPFVPLMAPRIRPSLDDEENNAIIIRGEIT 253

Query: 252 ATRDSNATVSEITPCVDEPRNSNSEDQ--INGRDFLLKRSYSFGFERSLASERMILEPAT 311
            +R +  T++  T    E R S  E       R   LKRSYSF  ER   SER+ +EPAT
Sbjct: 254 PSRSNWNTIAADTTNDQEIRASVEEQSSPAISRFRELKRSYSFECERESESERVTMEPAT 313

Query: 312 ASPWRYRRGSFWSKRPSPFGSL-PKARVFSFRYYRGMKSPFFRRRG----FFPLSESSWR 371
            SPWRYRR S W+KR SPFG+L  K+RVFSFRYYR  KSPFFRRR     F+P+SE    
Sbjct: 314 VSPWRYRR-STWNKRQSPFGNLISKSRVFSFRYYRSTKSPFFRRRSSAGVFYPISERI-- 373

Query: 372 FSNGAGGGSSRRTKSMTSPMFLRSSATGGGAAAFSSSRLRCGDPEALLSPERFNRR 404
               A G SSRRTKSMTSPMF R+       A  SSSRLRCGDPEALLSPER+ RR
Sbjct: 374 ---PATGSSSRRTKSMTSPMFFRT-------APHSSSRLRCGDPEALLSPERWRRR 404

BLAST of MS020508 vs. ExPASy Swiss-Prot
Match: Q9LY41 (E3 ubiquitin-protein ligase ATL4 OS=Arabidopsis thaliana OX=3702 GN=ATL4 PE=1 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 6.5e-19
Identity = 50/122 (40.98%), Postives = 73/122 (59.84%), Query Frame = 0

Query: 93  RSYLPSSSAGDLDSLPFDSPNFFEPADAFHVFSPYGLDESVIKTIPLSVYTAKSRRDS-- 152
           RS LP SS+  + ++  DS  F     + H  SP     SV+ ++P+  +++ +RR S  
Sbjct: 58  RSVLPLSSSSSVATVTSDSRRF-----SGHRVSPETERSSVLDSLPIFKFSSVTRRSSSM 117

Query: 153 RDRECAVCLLEFEDDDYVRTLPVCSHAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPV 212
              +CAVCL +FE +D +R LP+C HAFH DCID+WL S+  CPLCR+ +F  ES     
Sbjct: 118 NSGDCAVCLSKFEPEDQLRLLPLCCHAFHADCIDIWLVSNQTCPLCRSPLFASESDLMKS 174

BLAST of MS020508 vs. ExPASy Swiss-Prot
Match: Q9LZJ6 (RING-H2 finger protein ATL5 OS=Arabidopsis thaliana OX=3702 GN=ATL5 PE=2 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 8.5e-19
Identity = 65/158 (41.14%), Postives = 89/158 (56.33%), Query Frame = 0

Query: 67  LIPPILHLLRLH-HRRW---RRWRRRRRRARSYLPSSSAGDLDSLPFDSPNFFEPADAFH 126
           L   ++ +L  H + RW   R+ RR RRR  ++L S +A              +P  +  
Sbjct: 36  LFVAVILILCFHSYARWLFRRQNRRIRRRISAHLRSLAAAR------------DPTQSSS 95

Query: 127 VFSPYGLDESVIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPVCSHAFHVDC 186
             SP  LD +V++ IP+ VY+ K+  +S   EC+VCL EFE+DD  R LP C H FHVDC
Sbjct: 96  SLSP--LDPTVLEKIPIFVYSVKT-HESPLEECSVCLSEFEEDDEGRVLPKCGHVFHVDC 155

Query: 187 IDVWLRSHANCPLCRAVVFRPESPFT-PVMAARIRPSL 220
           ID W RS ++CPLCRA V +P  P T P   A + PS+
Sbjct: 156 IDTWFRSRSSCPLCRAPV-QPAQPVTEPEPVAAVFPSV 177

BLAST of MS020508 vs. ExPASy Swiss-Prot
Match: Q9SRQ8 (RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2)

HSP 1 Score: 94.4 bits (233), Expect = 3.2e-18
Identity = 67/191 (35.08%), Postives = 97/191 (50.79%), Query Frame = 0

Query: 24  FVPPSSL----QPPKLPVDFSPPLIAMVVVIATAFLIVTYSRLISRHLIPPILHLLRLHH 83
           F PP S     +      DFSP LIA++ ++A+AF++V+Y  LIS++            H
Sbjct: 37  FPPPPSFYLDDEDDSSSSDFSPLLIALIGILASAFILVSYYTLISKYC-----------H 96

Query: 84  RRWRRWRRRRRRARSYLPSSSAGDLDSLPFD----------SPNFFEPADAFHVFSPYGL 143
           RR           R    S+SA  ++ +  D          +     P          GL
Sbjct: 97  RR-----------RHNSSSTSAAAINRISSDYTWQGTNNNNNNGATNPNQTIGGGGGDGL 156

Query: 144 DESVIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPVCSHAFHVDCIDVWLRS 201
           DES+IK+I +  Y  K        +C+VCL EF++++ +R LP C+HAFHV CID WL+S
Sbjct: 157 DESLIKSITVYKY-RKMDGFVESSDCSVCLSEFQENESLRLLPKCNHAFHVPCIDTWLKS 204

BLAST of MS020508 vs. ExPASy Swiss-Prot
Match: O22255 (RING-H2 finger protein ATL64 OS=Arabidopsis thaliana OX=3702 GN=ATL64 PE=2 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 1.2e-17
Identity = 57/142 (40.14%), Postives = 77/142 (54.23%), Query Frame = 0

Query: 67  LIPPILHLLRLH-HRRW---RRWRRRRRRARSYLPSSSAGDLDSLPFDSPNFFEPADAFH 126
           L   ++ +L  H + RW   R  RR RRR RS+L + SA   D                 
Sbjct: 36  LFVAVIMILCFHSYARWLFRRHNRRIRRRIRSHLRTLSASPRDQ---------------- 95

Query: 127 VFSPYGLDESVIKTIPLSVYTAKSRRDSRDR-ECAVCLLEFEDDDYVRTLPVCSHAFHVD 186
                 LD++V+  IP+ VY++K+     ++ EC+VCL EFE++D  R LP C H+FHVD
Sbjct: 96  -----ALDQAVLDKIPIFVYSSKNPPPPEEKEECSVCLSEFEEEDEGRLLPKCGHSFHVD 155

Query: 187 CIDVWLRSHANCPLCRAVVFRP 204
           CID W RS + CPLCRA V  P
Sbjct: 156 CIDTWFRSRSTCPLCRAPVQPP 156

BLAST of MS020508 vs. ExPASy TrEMBL
Match: A0A6J1JBG1 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111484253 PE=3 SV=1)

HSP 1 Score: 691.8 bits (1784), Expect = 1.7e-195
Identity = 370/414 (89.37%), Postives = 379/414 (91.55%), Query Frame = 0

Query: 1   MPTAAVPSPSPSPSPSPSSS-----ALNFVPPSSLQPPKLPVDFSPPLIAMVVVIATAFL 60
           M TA  PSPSPSPSPS SSS     A+NF PPSSL P KLPVDFSPPLIAMVVVIATAFL
Sbjct: 1   MSTAPSPSPSPSPSPSTSSSSSSAAAVNFPPPSSLLPAKLPVDFSPPLIAMVVVIATAFL 60

Query: 61  IVTYSRLISRHLIPPILHLLRLHHRRWRRWRRRRRRARSYLPSSSAGDLDSLPFDSPNFF 120
           IVTYSRLISRH+IPPIL L+R H RRWRRWRRRRRRARSYLPSSS GDLDSLP+DSP FF
Sbjct: 61  IVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLPSSSGGDLDSLPYDSP-FF 120

Query: 121 EPADAFHVFSPYGLDESVIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPVCS 180
           E  D FHVFSPYGLDES+IKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLP+CS
Sbjct: 121 EHTDGFHVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPICS 180

Query: 181 HAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTILRSIVLEPLAGA 240
           HAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTIL+SIVLEPLA  
Sbjct: 181 HAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTILQSIVLEPLAEG 240

Query: 241 ---TRDS-NATVSEITPCVDEPRNSNS-EDQINGRDFLLKRSYSFGFERSLASERMILEP 300
               RDS NAT SEITP VDEPRNSNS EDQ+NGRDFLLKRSYSFGFERSLASERMILEP
Sbjct: 241 PIPNRDSYNATESEITPYVDEPRNSNSLEDQMNGRDFLLKRSYSFGFERSLASERMILEP 300

Query: 301 ATASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRR-GFFPLSESSWRFS 360
           ATASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRR GFFPLSESSWRFS
Sbjct: 301 ATASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFS 360

Query: 361 NGAGGGSSRRTKSMTSPMFLRSSATGGGAAAFSSSRLRCGDPEALLSPERFNRR 404
           NG GGGSSRR+KSMTSPMFLRSS T GGAA FSSSRLRCGDPEALLSPERFNRR
Sbjct: 361 NGVGGGSSRRSKSMTSPMFLRSSVTSGGAATFSSSRLRCGDPEALLSPERFNRR 413

BLAST of MS020508 vs. ExPASy TrEMBL
Match: A0A6J1FVL7 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111448502 PE=3 SV=1)

HSP 1 Score: 691.0 bits (1782), Expect = 2.9e-195
Identity = 368/414 (88.89%), Postives = 380/414 (91.79%), Query Frame = 0

Query: 1   MPTAAVPSPSPSPSPSPSSS-----ALNFVPPSSLQPPKLPVDFSPPLIAMVVVIATAFL 60
           M TA  PSPSPSPSPSPS+S     A+NF  PSSL P KLPVDFSPPLIAMVVVIATAFL
Sbjct: 1   MSTAPSPSPSPSPSPSPSTSSSAAAAVNFPSPSSLLPGKLPVDFSPPLIAMVVVIATAFL 60

Query: 61  IVTYSRLISRHLIPPILHLLRLHHRRWRRWRRRRRRARSYLPSSSAGDLDSLPFDSPNFF 120
           IVTYSRLISRH+IPPIL L+R H RRWRRWRRRRRRARSYLPSSS GDLDSLP+DSP FF
Sbjct: 61  IVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLPSSSGGDLDSLPYDSP-FF 120

Query: 121 EPADAFHVFSPYGLDESVIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPVCS 180
           E  D FHVFSPYGLDES+IKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLP+CS
Sbjct: 121 EHTDGFHVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPICS 180

Query: 181 HAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTILRSIVLEPLAGA 240
           HAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLD+TIL+SIVLEPLA  
Sbjct: 181 HAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDETILQSIVLEPLAEG 240

Query: 241 ---TRDS-NATVSEITPCVDEPRNSNS-EDQINGRDFLLKRSYSFGFERSLASERMILEP 300
               RDS +AT SEITPCVDEPRNSNS EDQ+NGRDFLLKRSYSFGFERSLASERMILEP
Sbjct: 241 PIPNRDSYHATESEITPCVDEPRNSNSLEDQMNGRDFLLKRSYSFGFERSLASERMILEP 300

Query: 301 ATASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRR-GFFPLSESSWRFS 360
           ATASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRR GFFPLSESSWRFS
Sbjct: 301 ATASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFS 360

Query: 361 NGAGGGSSRRTKSMTSPMFLRSSATGGGAAAFSSSRLRCGDPEALLSPERFNRR 404
           NG GGGSSRR+KSMTSPMFLRSS T GGAA FSSSRLRCGDPEALLSPERFNRR
Sbjct: 361 NGVGGGSSRRSKSMTSPMFLRSSVTSGGAATFSSSRLRCGDPEALLSPERFNRR 413

BLAST of MS020508 vs. ExPASy TrEMBL
Match: A0A0A0LIL3 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_2G238760 PE=3 SV=1)

HSP 1 Score: 684.5 bits (1765), Expect = 2.7e-193
Identity = 372/422 (88.15%), Postives = 382/422 (90.52%), Query Frame = 0

Query: 1   MPTAAVPSPSPSPSPSPSSS---------ALNFVPPSSLQPPKLPVDFSPPLIAMVVVIA 60
           MPT+  PSPSPSPSPSPS+S         A NF PPSSLQP KLPVDFSPPLIAMVVVIA
Sbjct: 1   MPTSPSPSPSPSPSPSPSTSSSSSSSSAAAANFPPPSSLQPAKLPVDFSPPLIAMVVVIA 60

Query: 61  TAFLIVTYSRLISRHLIPPILHLLRLHHRRWRRWRRRRR-RARSYLPSSSAGDLDSLPFD 120
           TAFLIVTYSRLISRHLIPPILHLLRLH RRWRRWRRRRR  ARSY+PSSS GDLDSLP+D
Sbjct: 61  TAFLIVTYSRLISRHLIPPILHLLRLHRRRWRRWRRRRRLAARSYIPSSSGGDLDSLPYD 120

Query: 121 SPNFFEP-ADAFHVFSPYGLDESVIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVR 180
           SP FFEP  DAFHVFSPYGLDESVIKTIPLSVYTAK    SRDRECAVCLLEFEDDDYVR
Sbjct: 121 SP-FFEPTTDAFHVFSPYGLDESVIKTIPLSVYTAK----SRDRECAVCLLEFEDDDYVR 180

Query: 181 TLPVCSHAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTILRSIVL 240
           TLP+CSHAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTILRSI+L
Sbjct: 181 TLPICSHAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTILRSIIL 240

Query: 241 EPLAGA----TRDS-NATVSEITPCVDEPRNSNS-EDQINGRDFLLKRSYSFGFERSLAS 300
           EPLA       RDS +AT SEITPC+DEPRNSNS EDQ+NGRDFLLKRSYSFGFERSLAS
Sbjct: 241 EPLAEGPPIPNRDSYHATESEITPCIDEPRNSNSVEDQMNGRDFLLKRSYSFGFERSLAS 300

Query: 301 ERMILEPATASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRR-GFFPLS 360
           ERMILEPAT SPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRR GFFPLS
Sbjct: 301 ERMILEPATTSPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLS 360

Query: 361 ESSWRFSNGAGGGSSRRTKSMTSPMFLRSSAT-GGGAAAFSSSRLRCGDPEALLSPERFN 404
           ESSWRFSNG GGGSSRR+KSMTSPMFLRSS T GGGAAAFSSSRLRCGDPEALLSPERFN
Sbjct: 361 ESSWRFSNGVGGGSSRRSKSMTSPMFLRSSVTGGGGAAAFSSSRLRCGDPEALLSPERFN 417

BLAST of MS020508 vs. ExPASy TrEMBL
Match: A0A1S4E2C5 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103498427 PE=3 SV=1)

HSP 1 Score: 673.3 bits (1736), Expect = 6.2e-190
Identity = 366/415 (88.19%), Postives = 378/415 (91.08%), Query Frame = 0

Query: 1   MPTAAVPSPSPSPSPSPSSSALNFVPPSSLQPPKLPVDFSPPLIAMVVVIATAFLIVTYS 60
           MPT+  PSPS SPS S +++A NF PPSSLQ  KLPVDFSPPLIAMVVVIATAFLIVTYS
Sbjct: 1   MPTSPSPSPSLSPSTS-AAAAANFPPPSSLQSAKLPVDFSPPLIAMVVVIATAFLIVTYS 60

Query: 61  RLISRHLIPPILHLLRLHHRRWRRWRRRRRR-ARSYLPSSSAGDLDSLPFDSPNFFEP-A 120
           RLISRHL+PPILHLLRLH RRWRRWRRRRRR ARSY+PSSS GDLDSLP+DSP FFEP  
Sbjct: 61  RLISRHLVPPILHLLRLHRRRWRRWRRRRRRAARSYIPSSSGGDLDSLPYDSP-FFEPTT 120

Query: 121 DAFHVFSPYGLDESVIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPVCSHAF 180
           DAFHVFSPYGLDESVIKTIPLSVYTAK    SRDRECAVCLLEFEDDDYVRTLP+CSHAF
Sbjct: 121 DAFHVFSPYGLDESVIKTIPLSVYTAK----SRDRECAVCLLEFEDDDYVRTLPICSHAF 180

Query: 181 HVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTILRSIVLEPLAGA--- 240
           HVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTILRSIVLEPLA     
Sbjct: 181 HVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTILRSIVLEPLAEGPLI 240

Query: 241 -TRDS-NATVSEITPCVDEPRNSNS-EDQINGRDFLLKRSYSFGFERSLASERMILEPAT 300
             RDS +AT SEITPC+DEPRNSNS EDQ+NGRDFLLKRSYSFGFERSLASERMILEPAT
Sbjct: 241 PNRDSYHATESEITPCIDEPRNSNSVEDQMNGRDFLLKRSYSFGFERSLASERMILEPAT 300

Query: 301 ASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRR-GFFPLSESSWRFSNG 360
            SPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRR GFFPLSESSWRFSNG
Sbjct: 301 TSPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFSNG 360

Query: 361 AGGGSSRRTKSMTSPMFLRSSAT---GGGAAAFSSSRLRCGDPEALLSPERFNRR 404
            GGGSSRR+KSMTSPMFLRSS T   GGG AAFSSSRLRCGDPEALLSPERFNRR
Sbjct: 361 VGGGSSRRSKSMTSPMFLRSSVTGGGGGGGAAFSSSRLRCGDPEALLSPERFNRR 409

BLAST of MS020508 vs. ExPASy TrEMBL
Match: A0A5A7TSC8 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold243G005120 PE=3 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 7.9e-177
Identity = 335/369 (90.79%), Postives = 342/369 (92.68%), Query Frame = 0

Query: 46  MVVVIATAFLIVTYSRLISRHLIPPILHLLRLHHRRWRRWRRRRRR-ARSYLPSSSAGDL 105
           MVVVIATAFLIVTYSRLISRHLIPPILHLLRLH RRWRRWRRRRRR ARSY+PSSS GDL
Sbjct: 1   MVVVIATAFLIVTYSRLISRHLIPPILHLLRLHRRRWRRWRRRRRRAARSYIPSSSGGDL 60

Query: 106 DSLPFDSPNFFEP-ADAFHVFSPYGLDESVIKTIPLSVYTAKSRRDSRDRECAVCLLEFE 165
           DSLP+DSP FFEP  DAFHVFSPYGLDESVIKTIPLSVYTAK    SRDRECAVCLLEFE
Sbjct: 61  DSLPYDSP-FFEPTTDAFHVFSPYGLDESVIKTIPLSVYTAK----SRDRECAVCLLEFE 120

Query: 166 DDDYVRTLPVCSHAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTI 225
           DDDYVRTLP+CSHAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTI
Sbjct: 121 DDDYVRTLPICSHAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTI 180

Query: 226 LRSIVLEPLAGA----TRDS-NATVSEITPCVDEPRNSNS-EDQINGRDFLLKRSYSFGF 285
           LRSIVLEPLA       RDS +AT SEITPC+DEPRNSNS EDQ+NGRDFLLKRSYSFGF
Sbjct: 181 LRSIVLEPLAEGPLIPNRDSYHATESEITPCIDEPRNSNSVEDQMNGRDFLLKRSYSFGF 240

Query: 286 ERSLASERMILEPATASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRR- 345
           ERSLASERMILEPAT SPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRR 
Sbjct: 241 ERSLASERMILEPATTSPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRG 300

Query: 346 GFFPLSESSWRFSNGAGGGSSRRTKSMTSPMFLRSSAT--GGGAAAFSSSRLRCGDPEAL 404
           GFFPLSESSWRFSNG GGGSSRR+KSMTSPMFLRSS T  GGG AAFSSSRLRCGDPEAL
Sbjct: 301 GFFPLSESSWRFSNGVGGGSSRRSKSMTSPMFLRSSVTGGGGGGAAFSSSRLRCGDPEAL 360

BLAST of MS020508 vs. TAIR 10
Match: AT3G18930.1 (RING/U-box superfamily protein )

HSP 1 Score: 341.3 bits (874), Expect = 1.1e-93
Identity = 224/416 (53.85%), Postives = 265/416 (63.70%), Query Frame = 0

Query: 12  SPSPSPSSSALNFVPPSSLQPPKLPVDFSPPLIAMVVVIATAFLIVTYSRLISRHLIPPI 71
           SPSPSPSS     +   S  P   P++FSPPLIAMVVV+A AFL VTYSRLISR  + P+
Sbjct: 14  SPSPSPSSGISEEILSRSSDP---PLEFSPPLIAMVVVLAAAFLFVTYSRLISRRFLSPL 73

Query: 72  LHLLRLHHRRWRRWRRRRRRARSYLPSSSAGDLDSLPFDSPNFFEPADAFHV--FSPYGL 131
                   RR+RRWR RRRR   +L S+S+    S    S + F P D+FH   +SPYGL
Sbjct: 74  F-------RRFRRWRCRRRRL-LHLSSASSASTSSSDLRSFSPF-PFDSFHYSSYSPYGL 133

Query: 132 DESVIKTIPLSVYTAKS---------RRDSRDRECAVCLLEFEDDDYVRTLPVCSHAFHV 191
           D+SVIKT+PL +Y+A +            +  R+CAVCLLEFE+ DYVRTLP+C HAFH+
Sbjct: 134 DDSVIKTLPLFLYSAAACTGKPAVGKTSAANCRDCAVCLLEFEEGDYVRTLPLCFHAFHL 193

Query: 192 DCIDVWLRSHANCPLCR------AVVFRPESPFTPVMAARIRPSLDDTILRSIVLEPLAG 251
           +CID WLRSH NCPLCR      A V  P SPF P+MA RIRPSLDD    +I++     
Sbjct: 194 ECIDEWLRSHPNCPLCRTAILGSAGVLTPMSPFVPLMAPRIRPSLDDEENNAIIIRGEIT 253

Query: 252 ATRDSNATVSEITPCVDEPRNSNSEDQ--INGRDFLLKRSYSFGFERSLASERMILEPAT 311
            +R +  T++  T    E R S  E       R   LKRSYSF  ER   SER+ +EPAT
Sbjct: 254 PSRSNWNTIAADTTNDQEIRASVEEQSSPAISRFRELKRSYSFECERESESERVTMEPAT 313

Query: 312 ASPWRYRRGSFWSKRPSPFGSL-PKARVFSFRYYRGMKSPFFRRRG----FFPLSESSWR 371
            SPWRYRR S W+KR SPFG+L  K+RVFSFRYYR  KSPFFRRR     F+P+SE    
Sbjct: 314 VSPWRYRR-STWNKRQSPFGNLISKSRVFSFRYYRSTKSPFFRRRSSAGVFYPISERI-- 373

Query: 372 FSNGAGGGSSRRTKSMTSPMFLRSSATGGGAAAFSSSRLRCGDPEALLSPERFNRR 404
               A G SSRRTKSMTSPMF R+       A  SSSRLRCGDPEALLSPER+ RR
Sbjct: 374 ---PATGSSSRRTKSMTSPMFFRT-------APHSSSRLRCGDPEALLSPERWRRR 404

BLAST of MS020508 vs. TAIR 10
Match: AT3G18930.2 (RING/U-box superfamily protein )

HSP 1 Score: 341.3 bits (874), Expect = 1.1e-93
Identity = 224/416 (53.85%), Postives = 265/416 (63.70%), Query Frame = 0

Query: 12  SPSPSPSSSALNFVPPSSLQPPKLPVDFSPPLIAMVVVIATAFLIVTYSRLISRHLIPPI 71
           SPSPSPSS     +   S  P   P++FSPPLIAMVVV+A AFL VTYSRLISR  + P+
Sbjct: 14  SPSPSPSSGISEEILSRSSDP---PLEFSPPLIAMVVVLAAAFLFVTYSRLISRRFLSPL 73

Query: 72  LHLLRLHHRRWRRWRRRRRRARSYLPSSSAGDLDSLPFDSPNFFEPADAFHV--FSPYGL 131
                   RR+RRWR RRRR   +L S+S+    S    S + F P D+FH   +SPYGL
Sbjct: 74  F-------RRFRRWRCRRRRL-LHLSSASSASTSSSDLRSFSPF-PFDSFHYSSYSPYGL 133

Query: 132 DESVIKTIPLSVYTAKS---------RRDSRDRECAVCLLEFEDDDYVRTLPVCSHAFHV 191
           D+SVIKT+PL +Y+A +            +  R+CAVCLLEFE+ DYVRTLP+C HAFH+
Sbjct: 134 DDSVIKTLPLFLYSAAACTGKPAVGKTSAANCRDCAVCLLEFEEGDYVRTLPLCFHAFHL 193

Query: 192 DCIDVWLRSHANCPLCR------AVVFRPESPFTPVMAARIRPSLDDTILRSIVLEPLAG 251
           +CID WLRSH NCPLCR      A V  P SPF P+MA RIRPSLDD    +I++     
Sbjct: 194 ECIDEWLRSHPNCPLCRTAILGSAGVLTPMSPFVPLMAPRIRPSLDDEENNAIIIRGEIT 253

Query: 252 ATRDSNATVSEITPCVDEPRNSNSEDQ--INGRDFLLKRSYSFGFERSLASERMILEPAT 311
            +R +  T++  T    E R S  E       R   LKRSYSF  ER   SER+ +EPAT
Sbjct: 254 PSRSNWNTIAADTTNDQEIRASVEEQSSPAISRFRELKRSYSFECERESESERVTMEPAT 313

Query: 312 ASPWRYRRGSFWSKRPSPFGSL-PKARVFSFRYYRGMKSPFFRRRG----FFPLSESSWR 371
            SPWRYRR S W+KR SPFG+L  K+RVFSFRYYR  KSPFFRRR     F+P+SE    
Sbjct: 314 VSPWRYRR-STWNKRQSPFGNLISKSRVFSFRYYRSTKSPFFRRRSSAGVFYPISERI-- 373

Query: 372 FSNGAGGGSSRRTKSMTSPMFLRSSATGGGAAAFSSSRLRCGDPEALLSPERFNRR 404
               A G SSRRTKSMTSPMF R+       A  SSSRLRCGDPEALLSPER+ RR
Sbjct: 374 ---PATGSSSRRTKSMTSPMFFRT-------APHSSSRLRCGDPEALLSPERWRRR 404

BLAST of MS020508 vs. TAIR 10
Match: AT3G60220.1 (TOXICOS EN LEVADURA 4 )

HSP 1 Score: 96.7 bits (239), Expect = 4.6e-20
Identity = 50/122 (40.98%), Postives = 73/122 (59.84%), Query Frame = 0

Query: 93  RSYLPSSSAGDLDSLPFDSPNFFEPADAFHVFSPYGLDESVIKTIPLSVYTAKSRRDS-- 152
           RS LP SS+  + ++  DS  F     + H  SP     SV+ ++P+  +++ +RR S  
Sbjct: 58  RSVLPLSSSSSVATVTSDSRRF-----SGHRVSPETERSSVLDSLPIFKFSSVTRRSSSM 117

Query: 153 RDRECAVCLLEFEDDDYVRTLPVCSHAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPV 212
              +CAVCL +FE +D +R LP+C HAFH DCID+WL S+  CPLCR+ +F  ES     
Sbjct: 118 NSGDCAVCLSKFEPEDQLRLLPLCCHAFHADCIDIWLVSNQTCPLCRSPLFASESDLMKS 174

BLAST of MS020508 vs. TAIR 10
Match: AT3G62690.1 (AtL5 )

HSP 1 Score: 96.3 bits (238), Expect = 6.0e-20
Identity = 65/158 (41.14%), Postives = 89/158 (56.33%), Query Frame = 0

Query: 67  LIPPILHLLRLH-HRRW---RRWRRRRRRARSYLPSSSAGDLDSLPFDSPNFFEPADAFH 126
           L   ++ +L  H + RW   R+ RR RRR  ++L S +A              +P  +  
Sbjct: 36  LFVAVILILCFHSYARWLFRRQNRRIRRRISAHLRSLAAAR------------DPTQSSS 95

Query: 127 VFSPYGLDESVIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPVCSHAFHVDC 186
             SP  LD +V++ IP+ VY+ K+  +S   EC+VCL EFE+DD  R LP C H FHVDC
Sbjct: 96  SLSP--LDPTVLEKIPIFVYSVKT-HESPLEECSVCLSEFEEDDEGRVLPKCGHVFHVDC 155

Query: 187 IDVWLRSHANCPLCRAVVFRPESPFT-PVMAARIRPSL 220
           ID W RS ++CPLCRA V +P  P T P   A + PS+
Sbjct: 156 IDTWFRSRSSCPLCRAPV-QPAQPVTEPEPVAAVFPSV 177

BLAST of MS020508 vs. TAIR 10
Match: AT3G03550.1 (RING/U-box superfamily protein )

HSP 1 Score: 94.4 bits (233), Expect = 2.3e-19
Identity = 67/191 (35.08%), Postives = 97/191 (50.79%), Query Frame = 0

Query: 24  FVPPSSL----QPPKLPVDFSPPLIAMVVVIATAFLIVTYSRLISRHLIPPILHLLRLHH 83
           F PP S     +      DFSP LIA++ ++A+AF++V+Y  LIS++            H
Sbjct: 37  FPPPPSFYLDDEDDSSSSDFSPLLIALIGILASAFILVSYYTLISKYC-----------H 96

Query: 84  RRWRRWRRRRRRARSYLPSSSAGDLDSLPFD----------SPNFFEPADAFHVFSPYGL 143
           RR           R    S+SA  ++ +  D          +     P          GL
Sbjct: 97  RR-----------RHNSSSTSAAAINRISSDYTWQGTNNNNNNGATNPNQTIGGGGGDGL 156

Query: 144 DESVIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPVCSHAFHVDCIDVWLRS 201
           DES+IK+I +  Y  K        +C+VCL EF++++ +R LP C+HAFHV CID WL+S
Sbjct: 157 DESLIKSITVYKY-RKMDGFVESSDCSVCLSEFQENESLRLLPKCNHAFHVPCIDTWLKS 204

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038902895.12.9e-19790.95RING-H2 finger protein ATL65 [Benincasa hispida][more]
XP_022986546.13.5e-19589.37RING-H2 finger protein ATL65 [Cucurbita maxima][more]
XP_022943929.16.0e-19588.89RING-H2 finger protein ATL65 [Cucurbita moschata] >KAG7010952.1 RING-H2 finger p... [more]
XP_023511773.16.0e-19588.67RING-H2 finger protein ATL65 [Cucurbita pepo subsp. pepo][more]
KAG6571140.11.3e-19488.65RING-H2 finger protein ATL65, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q67YI61.5e-9253.85RING-H2 finger protein ATL65 OS=Arabidopsis thaliana OX=3702 GN=ATL65 PE=2 SV=2[more]
Q9LY416.5e-1940.98E3 ubiquitin-protein ligase ATL4 OS=Arabidopsis thaliana OX=3702 GN=ATL4 PE=1 SV... [more]
Q9LZJ68.5e-1941.14RING-H2 finger protein ATL5 OS=Arabidopsis thaliana OX=3702 GN=ATL5 PE=2 SV=1[more]
Q9SRQ83.2e-1835.08RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2[more]
O222551.2e-1740.14RING-H2 finger protein ATL64 OS=Arabidopsis thaliana OX=3702 GN=ATL64 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JBG11.7e-19589.37RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111484253 P... [more]
A0A6J1FVL72.9e-19588.89RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111448502... [more]
A0A0A0LIL32.7e-19388.15RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_2G238760 PE... [more]
A0A1S4E2C56.2e-19088.19RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103498427 PE=3 ... [more]
A0A5A7TSC87.9e-17790.79RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
Match NameE-valueIdentityDescription
AT3G18930.11.1e-9353.85RING/U-box superfamily protein [more]
AT3G18930.21.1e-9353.85RING/U-box superfamily protein [more]
AT3G60220.14.6e-2040.98TOXICOS EN LEVADURA 4 [more]
AT3G62690.16.0e-2041.14AtL5 [more]
AT3G03550.12.3e-1935.08RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 155..196
e-value: 1.1E-6
score: 38.3
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 154..197
e-value: 1.6E-13
score: 50.8
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 155..197
score: 12.208971
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 120..201
e-value: 3.0E-22
score: 80.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..25
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..16
NoneNo IPR availablePANTHERPTHR45676:SF74TRANSCRIPTION FACTOR C2H2 FAMILY-RELATEDcoord: 6..374
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 154..197
e-value: 6.44537E-20
score: 80.4237
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 146..203
IPR044675E3 ubiquitin-protein ligase RING1-likePANTHERPTHR45676RING-H2 FINGER PROTEIN ATL51-RELATEDcoord: 6..374

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS020508.1MS020508.1mRNA