MS020487 (gene) Bitter gourd (TR) v1

Overview


Name	MS020487
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Phytocyanin domain-containing protein
Location	scaffold375: 572599 .. 573042 (-)
RNA-Seq Expression	MS020487
Synteny	MS020487

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CCTAGAAAGATCATAGTGGGTGGCTCGGAGAATTGGCATCTTGGCTTCGACTACAGCAACTGGGCCCTTAAAAACGGTCCATTTTTTCTCAACGACATTCTAGGTAAACTTACACCAACAGTACTTTGATTTGACCTCACAGTTCGTTAGAAAATTCCCATCACAGAACTTATTGAAAGTTGTAATTTTGCAGTGTTCAAGTATGATCCTCCAACGGGCGCAACAGTTCCCCACAGTGTGTACTTGCTATCAAACATGCAAAGCTTCTCAAACTGTGATCTGAGAAGGGCCACAAAGCTTGCAAATTGGACACAAGGAAATGGCGATGGCTTCCAATTTGTGCTCAAACAACAAAAAACTTACTACTTTGCCTGTGGAGAAGGCAATGGCTTCCATTGCAAAAATGGATCCATGAAGTTCTCTGTCACACCAATACTTCAGAGT

mRNA sequence

CCTAGAAAGATCATAGTGGGTGGCTCGGAGAATTGGCATCTTGGCTTCGACTACAGCAACTGGGCCCTTAAAAACGGTCCATTTTTTCTCAACGACATTCTAGTGTTCAAGTATGATCCTCCAACGGGCGCAACAGTTCCCCACAGTGTGTACTTGCTATCAAACATGCAAAGCTTCTCAAACTGTGATCTGAGAAGGGCCACAAAGCTTGCAAATTGGACACAAGGAAATGGCGATGGCTTCCAATTTGTGCTCAAACAACAAAAAACTTACTACTTTGCCTGTGGAGAAGGCAATGGCTTCCATTGCAAAAATGGATCCATGAAGTTCTCTGTCACACCAATACTTCAGAGT

Coding sequence (CDS)

CCTAGAAAGATCATAGTGGGTGGCTCGGAGAATTGGCATCTTGGCTTCGACTACAGCAACTGGGCCCTTAAAAACGGTCCATTTTTTCTCAACGACATTCTAGTGTTCAAGTATGATCCTCCAACGGGCGCAACAGTTCCCCACAGTGTGTACTTGCTATCAAACATGCAAAGCTTCTCAAACTGTGATCTGAGAAGGGCCACAAAGCTTGCAAATTGGACACAAGGAAATGGCGATGGCTTCCAATTTGTGCTCAAACAACAAAAAACTTACTACTTTGCCTGTGGAGAAGGCAATGGCTTCCATTGCAAAAATGGATCCATGAAGTTCTCTGTCACACCAATACTTCAGAGT

Protein sequence

PRKIIVGGSENWHLGFDYSNWALKNGPFFLNDILVFKYDPPTGATVPHSVYLLSNMQSFSNCDLRRATKLANWTQGNGDGFQFVLKQQKTYYFACGEGNGFHCKNGSMKFSVTPILQS

Homology

[Close]

BLAST of MS020487 vs. NCBI nr
Match: XP_022148634.1 (leucine-rich repeat extensin-like protein 3 [Momordica charantia])

HSP 1 Score: 261.2 bits (666), Expect = 4.5e-66
Identity = 118/118 (100.00%), Postives = 118/118 (100.00%), Query Frame = 0


Query: 1   PRKIIVGGSENWHLGFDYSNWALKNGPFFLNDILVFKYDPPTGATVPHSVYLLSNMQSFS 60
           PRKIIVGGSENWHLGFDYSNWALKNGPFFLNDILVFKYDPPTGATVPHSVYLLSNMQSFS
Sbjct: 224 PRKIIVGGSENWHLGFDYSNWALKNGPFFLNDILVFKYDPPTGATVPHSVYLLSNMQSFS 283

Query: 61  NCDLRRATKLANWTQGNGDGFQFVLKQQKTYYFACGEGNGFHCKNGSMKFSVTPILQS 119
           NCDLRRATKLANWTQGNGDGFQFVLKQQKTYYFACGEGNGFHCKNGSMKFSVTPILQS
Sbjct: 284 NCDLRRATKLANWTQGNGDGFQFVLKQQKTYYFACGEGNGFHCKNGSMKFSVTPILQS 341

[Close]

BLAST of MS020487 vs. NCBI nr
Match: XP_008459215.1 (PREDICTED: extensin, partial [Cucumis melo])

HSP 1 Score: 201.4 bits (511), Expect = 4.2e-48
Identity = 88/117 (75.21%), Postives = 101/117 (86.32%), Query Frame = 0


Query: 1   PRKIIVGGSENWHLGFDYSNWALKNGPFFLNDILVFKYDPPTGATVPHSVYLLSNMQSFS 60
           PRKIIVGGS  W LGFDY++WALKNGPF++NDILVFKYDPP  +T PHSVYLL NM+SF+
Sbjct: 38  PRKIIVGGSNQWQLGFDYTDWALKNGPFYVNDILVFKYDPPNMSTPPHSVYLLPNMRSFA 97

Query: 61  NCDLRRATKLANWTQGNGDGFQFVLKQQKTYYFACGEGNGFHCKNGSMKFSVTPILQ 118
           NCDL +A  LAN  QG+GDGF+FVLK Q  YYFACGEGNGFHCK GSMKF++TPIL+
Sbjct: 98  NCDLGKAKMLANIKQGSGDGFEFVLKDQNPYYFACGEGNGFHCKLGSMKFTITPILK 154

[Close]

BLAST of MS020487 vs. NCBI nr
Match: XP_022148560.1 (uncharacterized protein LOC111017194 [Momordica charantia])

HSP 1 Score: 200.3 bits (508), Expect = 9.3e-48
Identity = 86/115 (74.78%), Postives = 97/115 (84.35%), Query Frame = 0


Query: 2   RKIIVGGSENWHLGFDYSNWALKNGPFFLNDILVFKYDPPTGATVPHSVYLLSNMQSFSN 61
           +KI+VGGSENW  GFDY+NWALKNGPF++ND LVFKYDPP   T PHSVYLL N+ SFS 
Sbjct: 73  KKIMVGGSENWRFGFDYNNWALKNGPFYINDTLVFKYDPPNSTTFPHSVYLLPNLGSFSE 132

Query: 62  CDLRRATKLANWTQGNGDGFQFVLKQQKTYYFACGEGNGFHCKNGSMKFSVTPIL 117
           CDLR A K+ANWTQG GDGF+FVL+Q K YYFACGE NGFHCK G+MKFS+TP L
Sbjct: 133 CDLRAAQKVANWTQGGGDGFEFVLQQSKPYYFACGERNGFHCKVGTMKFSLTPQL 187

[Close]

BLAST of MS020487 vs. NCBI nr
Match: XP_011649199.1 (leucine-rich repeat extensin-like protein 3 [Cucumis sativus] >KGN61733.1 hypothetical protein Csa_006192 [Cucumis sativus])

HSP 1 Score: 198.4 bits (503), Expect = 3.5e-47
Identity = 86/118 (72.88%), Postives = 101/118 (85.59%), Query Frame = 0


Query: 1   PRKIIVGGSENWHLGFDYSNWALKNGPFFLNDILVFKYDPPTGATVPHSVYLLSNMQSFS 60
           PRKIIVGGS  W LGFDY++WALKNGPF++NDILVFKYDPP  +T PH+VYLL NM+S +
Sbjct: 177 PRKIIVGGSNQWQLGFDYTDWALKNGPFYVNDILVFKYDPPNRSTPPHNVYLLPNMRSLA 236

Query: 61  NCDLRRATKLANWTQGNGDGFQFVLKQQKTYYFACGEGNGFHCKNGSMKFSVTPILQS 119
           NCD  +A  LAN TQG+GDGF+FVLK Q  YYFACGEGNGFHCK GSMKF++TPIL++
Sbjct: 237 NCDFGKAKVLANITQGSGDGFEFVLKDQNPYYFACGEGNGFHCKLGSMKFTLTPILKA 294

[Close]

BLAST of MS020487 vs. NCBI nr
Match: XP_022947109.1 (extensin-like isoform X4 [Cucurbita moschata])

HSP 1 Score: 196.1 bits (497), Expect = 1.8e-46
Identity = 85/114 (74.56%), Postives = 97/114 (85.09%), Query Frame = 0


Query: 1   PRKIIVGGSENWHLGFDYSNWALKNGPFFLNDILVFKYDPPTGATVPHSVYLLSNMQSFS 60
           PRKIIVGGS+ W LGFDY++W LKNGPF++NDILVFKYDPP  +T PH+VYLL NMQS +
Sbjct: 132 PRKIIVGGSKYWRLGFDYNDWILKNGPFYVNDILVFKYDPPNSSTPPHNVYLLPNMQSLN 191

Query: 61  NCDLRRATKLANWTQGNGDGFQFVLKQQKTYYFACGEGNGFHCKNGSMKFSVTP 115
            CD RRA  +AN TQG+G+GF FVLKQQK YYFACGEGNGFHC  GSMKFS+TP
Sbjct: 192 KCDFRRAKLVANLTQGSGNGFNFVLKQQKAYYFACGEGNGFHCNLGSMKFSITP 245

[Close]

BLAST of MS020487 vs. ExPASy TrEMBL
Match: A0A6J1D4K0 (leucine-rich repeat extensin-like protein 3 OS=Momordica charantia OX=3673 GN=LOC111017245 PE=4 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 2.2e-66
Identity = 118/118 (100.00%), Postives = 118/118 (100.00%), Query Frame = 0


Query: 1   PRKIIVGGSENWHLGFDYSNWALKNGPFFLNDILVFKYDPPTGATVPHSVYLLSNMQSFS 60
           PRKIIVGGSENWHLGFDYSNWALKNGPFFLNDILVFKYDPPTGATVPHSVYLLSNMQSFS
Sbjct: 224 PRKIIVGGSENWHLGFDYSNWALKNGPFFLNDILVFKYDPPTGATVPHSVYLLSNMQSFS 283

Query: 61  NCDLRRATKLANWTQGNGDGFQFVLKQQKTYYFACGEGNGFHCKNGSMKFSVTPILQS 119
           NCDLRRATKLANWTQGNGDGFQFVLKQQKTYYFACGEGNGFHCKNGSMKFSVTPILQS
Sbjct: 284 NCDLRRATKLANWTQGNGDGFQFVLKQQKTYYFACGEGNGFHCKNGSMKFSVTPILQS 341

[Close]

BLAST of MS020487 vs. ExPASy TrEMBL
Match: A0A1S3CAV5 (extensin OS=Cucumis melo OX=3656 GN=LOC103498405 PE=4 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 2.0e-48
Identity = 88/117 (75.21%), Postives = 101/117 (86.32%), Query Frame = 0


Query: 1   PRKIIVGGSENWHLGFDYSNWALKNGPFFLNDILVFKYDPPTGATVPHSVYLLSNMQSFS 60
           PRKIIVGGS  W LGFDY++WALKNGPF++NDILVFKYDPP  +T PHSVYLL NM+SF+
Sbjct: 38  PRKIIVGGSNQWQLGFDYTDWALKNGPFYVNDILVFKYDPPNMSTPPHSVYLLPNMRSFA 97

Query: 61  NCDLRRATKLANWTQGNGDGFQFVLKQQKTYYFACGEGNGFHCKNGSMKFSVTPILQ 118
           NCDL +A  LAN  QG+GDGF+FVLK Q  YYFACGEGNGFHCK GSMKF++TPIL+
Sbjct: 98  NCDLGKAKMLANIKQGSGDGFEFVLKDQNPYYFACGEGNGFHCKLGSMKFTITPILK 154

[Close]

BLAST of MS020487 vs. ExPASy TrEMBL
Match: A0A6J1D388 (uncharacterized protein LOC111017194 OS=Momordica charantia OX=3673 GN=LOC111017194 PE=4 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 4.5e-48
Identity = 86/115 (74.78%), Postives = 97/115 (84.35%), Query Frame = 0


Query: 2   RKIIVGGSENWHLGFDYSNWALKNGPFFLNDILVFKYDPPTGATVPHSVYLLSNMQSFSN 61
           +KI+VGGSENW  GFDY+NWALKNGPF++ND LVFKYDPP   T PHSVYLL N+ SFS 
Sbjct: 73  KKIMVGGSENWRFGFDYNNWALKNGPFYINDTLVFKYDPPNSTTFPHSVYLLPNLGSFSE 132

Query: 62  CDLRRATKLANWTQGNGDGFQFVLKQQKTYYFACGEGNGFHCKNGSMKFSVTPIL 117
           CDLR A K+ANWTQG GDGF+FVL+Q K YYFACGE NGFHCK G+MKFS+TP L
Sbjct: 133 CDLRAAQKVANWTQGGGDGFEFVLQQSKPYYFACGERNGFHCKVGTMKFSLTPQL 187

[Close]

BLAST of MS020487 vs. ExPASy TrEMBL
Match: A0A0A0LIT9 (Phytocyanin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G234585 PE=4 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 1.7e-47
Identity = 86/118 (72.88%), Postives = 101/118 (85.59%), Query Frame = 0


Query: 1   PRKIIVGGSENWHLGFDYSNWALKNGPFFLNDILVFKYDPPTGATVPHSVYLLSNMQSFS 60
           PRKIIVGGS  W LGFDY++WALKNGPF++NDILVFKYDPP  +T PH+VYLL NM+S +
Sbjct: 177 PRKIIVGGSNQWQLGFDYTDWALKNGPFYVNDILVFKYDPPNRSTPPHNVYLLPNMRSLA 236

Query: 61  NCDLRRATKLANWTQGNGDGFQFVLKQQKTYYFACGEGNGFHCKNGSMKFSVTPILQS 119
           NCD  +A  LAN TQG+GDGF+FVLK Q  YYFACGEGNGFHCK GSMKF++TPIL++
Sbjct: 237 NCDFGKAKVLANITQGSGDGFEFVLKDQNPYYFACGEGNGFHCKLGSMKFTLTPILKA 294

[Close]

BLAST of MS020487 vs. ExPASy TrEMBL
Match: A0A6J1G5T9 (extensin-like isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111451078 PE=4 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 8.5e-47
Identity = 85/114 (74.56%), Postives = 97/114 (85.09%), Query Frame = 0


Query: 1   PRKIIVGGSENWHLGFDYSNWALKNGPFFLNDILVFKYDPPTGATVPHSVYLLSNMQSFS 60
           PRKIIVGGS+ W LGFDY++W LKNGPF++NDILVFKYDPP  +T PH+VYLL NMQS +
Sbjct: 132 PRKIIVGGSKYWRLGFDYNDWILKNGPFYVNDILVFKYDPPNSSTPPHNVYLLPNMQSLN 191

Query: 61  NCDLRRATKLANWTQGNGDGFQFVLKQQKTYYFACGEGNGFHCKNGSMKFSVTP 115
            CD RRA  +AN TQG+G+GF FVLKQQK YYFACGEGNGFHC  GSMKFS+TP
Sbjct: 192 KCDFRRAKLVANLTQGSGNGFNFVLKQQKAYYFACGEGNGFHCNLGSMKFSITP 245

[Close]

BLAST of MS020487 vs. TAIR 10
Match: AT2G15780.1 (Cupredoxin superfamily protein )

HSP 1 Score: 153.3 bits (386), Expect = 1.2e-37
Identity = 68/116 (58.62%), Postives = 85/116 (73.28%), Query Frame = 0


Query: 1   PRKIIVGGSENWHLGFDYSNWALKNGPFFLNDILVFKYDPPTGATVPHSVYLLSNMQSFS 60
           PRKIIVGG + W  GF+Y++WA K  PFFLNDILVFKY+PP  A   HSVYLL N  S+ 
Sbjct: 141 PRKIIVGGDKEWTYGFNYADWASKTAPFFLNDILVFKYNPP--APFTHSVYLLPNPSSYE 200

Query: 61  NCDLRRATKLANWTQGNGDGFQFVLKQQKTYYFACGEGNGFHCKNGSMKFSVTPIL 117
            CD+++   +A+  QG G GF+FVLKQ K YY +CGE +G HC NG+MKF+V P+L
Sbjct: 201 KCDVKKGKMIASPKQGAGKGFEFVLKQMKPYYISCGEHDGAHCSNGTMKFTVMPML 254

[Close]

BLAST of MS020487 vs. TAIR 10
Match: AT2G15770.1 (Cupredoxin superfamily protein )

HSP 1 Score: 127.1 bits (318), Expect = 9.4e-30
Identity = 54/116 (46.55%), Postives = 81/116 (69.83%), Query Frame = 0


Query: 1   PRKIIVGGSENWHLGFDYSNWALKNGPFFLNDILVFKYDPPTGATVPHSVYLLSNMQSFS 60
           P+KIIVGGS+ W  G DY +WA KN PF++ND+LVFKYD    A   ++VYL  +  S+ 
Sbjct: 143 PKKIIVGGSDGWKKGLDYKDWASKNAPFYVNDVLVFKYD--KSAKRRNNVYLFKDRWSYM 202

Query: 61  NCDLRRATKLANWTQGNGDGFQFVLKQQKTYYFACGEGNGFHCKNGSMKFSVTPIL 117
           NCD++ A K+ +  +G+ + F F LK+ + Y+FA GE +G +C+N +MKF++ P+L
Sbjct: 203 NCDIKNARKIGSTRKGSEESFNFTLKKIQPYFFASGEHDGDYCRNHNMKFTIFPVL 256

[Close]

BLAST of MS020487 vs. TAIR 10
Match: AT4G33930.1 (Cupredoxin superfamily protein )

HSP 1 Score: 86.7 bits (213), Expect = 1.4e-17
Identity = 46/112 (41.07%), Postives = 66/112 (58.93%), Query Frame = 0


Query: 12  WHLGFDYSNWALKNGPFFLNDILVFKY--DPPTGATVPH------SVYLLSNMQSFSNCD 71
           W  G+ Y+ W  K+ PF+++D+LVFKY  D  T +   H       VYLL +M+SF  C+
Sbjct: 229 WKNGYGYTEWTAKHAPFYVSDVLVFKYNNDDQTQSKTKHRNKKKNDVYLLPDMKSFKRCN 288

Query: 72  LRRATKLANWTQGNGDGFQFVLKQQKTYYFACGEGNGFHCKNGSMKFSVTPI 116
           + R  KL      +  GF+ +L++ +TYYFA G+ N   C N +MKFSV PI
Sbjct: 289 VARGKKLVARGGSSSRGFKLLLRKVQTYYFASGDHN--EC-NHNMKFSVHPI 337

[Close]

BLAST of MS020487 vs. TAIR 10
Match: AT4G34300.1 (Cupredoxin superfamily protein )

HSP 1 Score: 82.0 bits (201), Expect = 3.5e-16
Identity = 45/113 (39.82%), Postives = 62/113 (54.87%), Query Frame = 0


Query: 12  WHLGFDYSNWALKNGPFFLNDILVFKY---DPPTGATVPH------SVYLLSNMQSFSNC 71
           W  G+ Y+ W  K+ PF++ND+LVF Y   D     T  H       VYLL +M+SF  C
Sbjct: 198 WKNGYGYTEWTAKHAPFYVNDVLVFTYNNNDQTQSKTKHHHDKKKNDVYLLPDMKSFKRC 257

Query: 72  DLRRATKLANWTQGNGDGFQFVLKQQKTYYFACGEGNGFHCKNGSMKFSVTPI 116
           ++ R  KL      +  GF+ +L++  TYYF  G+ N   C N +MKFSV PI
Sbjct: 258 NVARGKKLVARGGSSSRGFKLLLRKVHTYYFVSGDHN--DC-NHNMKFSVHPI 307

The following BLAST results are available for this feature:

BLAST of MS020487 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148634.1	4.5e-66	100.00	leucine-rich repeat extensin-like protein 3 [Momordica charantia]	[more]
XP_008459215.1	4.2e-48	75.21	PREDICTED: extensin, partial [Cucumis melo]	[more]
XP_022148560.1	9.3e-48	74.78	uncharacterized protein LOC111017194 [Momordica charantia]	[more]
XP_011649199.1	3.5e-47	72.88	leucine-rich repeat extensin-like protein 3 [Cucumis sativus] >KGN61733.1 hypoth...	[more]
XP_022947109.1	1.8e-46	74.56	extensin-like isoform X4 [Cucurbita moschata]	[more]

BLAST of MS020487 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS020487 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D4K0	2.2e-66	100.00	leucine-rich repeat extensin-like protein 3 OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A1S3CAV5	2.0e-48	75.21	extensin OS=Cucumis melo OX=3656 GN=LOC103498405 PE=4 SV=1	[more]
A0A6J1D388	4.5e-48	74.78	uncharacterized protein LOC111017194 OS=Momordica charantia OX=3673 GN=LOC111017...	[more]
A0A0A0LIT9	1.7e-47	72.88	Phytocyanin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G234585...	[more]
A0A6J1G5T9	8.5e-47	74.56	extensin-like isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111451078 PE=4 SV=1	[more]

BLAST of MS020487 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G15780.1	1.2e-37	58.62	Cupredoxin superfamily protein	[more]
AT2G15770.1	9.4e-30	46.55	Cupredoxin superfamily protein	[more]
AT4G33930.1	1.4e-17	41.07	Cupredoxin superfamily protein	[more]
AT4G34300.1	3.5e-16	39.82	Cupredoxin superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003245	Phytocyanin domain	PFAM	PF02298	Cu_bind_like	coord: 15..106 e-value: 6.3E-7 score: 29.3
IPR003245	Phytocyanin domain	PROSITE	PS51485	PHYTOCYANIN	coord: 2..116 score: 25.327795
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 2..115 e-value: 5.3E-23 score: 83.3
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 3..114
None	No IPR available	PANTHER	PTHR34052	GLYCINE-RICH PROTEIN-LIKE	coord: 1..117
None	No IPR available	PANTHER	PTHR34052:SF1	GLYCINE-RICH PROTEIN-LIKE	coord: 1..117

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS020487.1	MS020487.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0009055	electron transfer activity