MS020480 (gene) Bitter gourd (TR) v1

Overview
NameMS020480
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionAlpha/beta-Hydrolases superfamily protein, putative
Locationscaffold375: 528329 .. 529231 (-)
RNA-Seq ExpressionMS020480
SyntenyMS020480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCCGACGCCGCCGACGAAGTAGTTTTCGAATTCCAACCGGTCTTCCGCGTCTTCAGAAGCGGCCGCGTCGAGAGGCTCATTCCCGGCGACCGCGTTCCGCCGTCGACTGATCAGCGGACCGGCGTGCAGTCCAAAGACATCCTCATCGATCCACAAACTGGAGTCTCCGTCCGCATTTACCTCCCGCCGGTTCCGGATTCAACAAAGCTCCCGCTCCTAATTTACATCCACGGCGGCGCATTCGCAATCGGCTCCGCCGATTCCGCCATCTACCACCGCCACCTGATCTCCCTCGCCGCCGAAGCGAACGCCGTCGCTCTGTCGGTGAACTACCGGCTGGCGCCTGAGCATCCTCTCCCTGCGGCGTACGACGACGCCTGGACGTCGGTGGAATGGGCCGCCTCGCACGCGAATCGCGACGGCGCGGAGATTTGGCTGAACAACCACGCGGATTTCGATCGCGTTTTCCTTGTTGGAGACAGCGCTGGGGCCAACATTTCGTACAACTTAGTGGGCCGGGCTGGTAATGGGCCGGCGGGATTGAACATTGTTGGGCTGGGCCTGATCCATCCGTTTTTCATGATAGACAAGCCCGATAAGCTGATCGAGTACATCTTCCCGACGTCGGCGGGACTGGAGGATCCGAGGATGAACCCGAGAGCGAACCCGGAACTACGGAGCCTACGGTGCACCCAGGTGGTGATACTCGTGGCGGAGAATGATTGGCTGAAGGATAGGGGGCGTGGGTTTCACGCGGCGCTGAGGGAGAGTGGGTGGAAGGGGTCGGTGGAGATTGTGGAAACGGAGGGGGAGGGCCACGTGTTCCATTTGTTTAATCCGGAGAACGAGAAGGCTGCTACGTTGGTGAAACAGGTGGCTTCCTTCATCCAATCAACG

mRNA sequence

ATGGATTCCGACGCCGCCGACGAAGTAGTTTTCGAATTCCAACCGGTCTTCCGCGTCTTCAGAAGCGGCCGCGTCGAGAGGCTCATTCCCGGCGACCGCGTTCCGCCGTCGACTGATCAGCGGACCGGCGTGCAGTCCAAAGACATCCTCATCGATCCACAAACTGGAGTCTCCGTCCGCATTTACCTCCCGCCGGTTCCGGATTCAACAAAGCTCCCGCTCCTAATTTACATCCACGGCGGCGCATTCGCAATCGGCTCCGCCGATTCCGCCATCTACCACCGCCACCTGATCTCCCTCGCCGCCGAAGCGAACGCCGTCGCTCTGTCGGTGAACTACCGGCTGGCGCCTGAGCATCCTCTCCCTGCGGCGTACGACGACGCCTGGACGTCGGTGGAATGGGCCGCCTCGCACGCGAATCGCGACGGCGCGGAGATTTGGCTGAACAACCACGCGGATTTCGATCGCGTTTTCCTTGTTGGAGACAGCGCTGGGGCCAACATTTCGTACAACTTAGTGGGCCGGGCTGGTAATGGGCCGGCGGGATTGAACATTGTTGGGCTGGGCCTGATCCATCCGTTTTTCATGATAGACAAGCCCGATAAGCTGATCGAGTACATCTTCCCGACGTCGGCGGGACTGGAGGATCCGAGGATGAACCCGAGAGCGAACCCGGAACTACGGAGCCTACGGTGCACCCAGGTGGTGATACTCGTGGCGGAGAATGATTGGCTGAAGGATAGGGGGCGTGGGTTTCACGCGGCGCTGAGGGAGAGTGGGTGGAAGGGGTCGGTGGAGATTGTGGAAACGGAGGGGGAGGGCCACGTGTTCCATTTGTTTAATCCGGAGAACGAGAAGGCTGCTACGTTGGTGAAACAGGTGGCTTCCTTCATCCAATCAACG

Coding sequence (CDS)

ATGGATTCCGACGCCGCCGACGAAGTAGTTTTCGAATTCCAACCGGTCTTCCGCGTCTTCAGAAGCGGCCGCGTCGAGAGGCTCATTCCCGGCGACCGCGTTCCGCCGTCGACTGATCAGCGGACCGGCGTGCAGTCCAAAGACATCCTCATCGATCCACAAACTGGAGTCTCCGTCCGCATTTACCTCCCGCCGGTTCCGGATTCAACAAAGCTCCCGCTCCTAATTTACATCCACGGCGGCGCATTCGCAATCGGCTCCGCCGATTCCGCCATCTACCACCGCCACCTGATCTCCCTCGCCGCCGAAGCGAACGCCGTCGCTCTGTCGGTGAACTACCGGCTGGCGCCTGAGCATCCTCTCCCTGCGGCGTACGACGACGCCTGGACGTCGGTGGAATGGGCCGCCTCGCACGCGAATCGCGACGGCGCGGAGATTTGGCTGAACAACCACGCGGATTTCGATCGCGTTTTCCTTGTTGGAGACAGCGCTGGGGCCAACATTTCGTACAACTTAGTGGGCCGGGCTGGTAATGGGCCGGCGGGATTGAACATTGTTGGGCTGGGCCTGATCCATCCGTTTTTCATGATAGACAAGCCCGATAAGCTGATCGAGTACATCTTCCCGACGTCGGCGGGACTGGAGGATCCGAGGATGAACCCGAGAGCGAACCCGGAACTACGGAGCCTACGGTGCACCCAGGTGGTGATACTCGTGGCGGAGAATGATTGGCTGAAGGATAGGGGGCGTGGGTTTCACGCGGCGCTGAGGGAGAGTGGGTGGAAGGGGTCGGTGGAGATTGTGGAAACGGAGGGGGAGGGCCACGTGTTCCATTTGTTTAATCCGGAGAACGAGAAGGCTGCTACGTTGGTGAAACAGGTGGCTTCCTTCATCCAATCAACG

Protein sequence

MDSDAADEVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLPPVPDSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPAAYDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGNGPAGLNIVGLGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVVILVAENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVASFIQST
Homology
BLAST of MS020480 vs. NCBI nr
Match: XP_022148541.1 (probable carboxylesterase 2 [Momordica charantia])

HSP 1 Score: 612.1 bits (1577), Expect = 2.6e-171
Identity = 301/301 (100.00%), Postives = 301/301 (100.00%), Query Frame = 0

Query: 1   MDSDAADEVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVR 60
           MDSDAADEVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVR
Sbjct: 1   MDSDAADEVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVR 60

Query: 61  IYLPPVPDSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHP 120
           IYLPPVPDSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHP
Sbjct: 61  IYLPPVPDSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHP 120

Query: 121 LPAAYDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGNGP 180
           LPAAYDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGNGP
Sbjct: 121 LPAAYDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGNGP 180

Query: 181 AGLNIVGLGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVVILVA 240
           AGLNIVGLGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVVILVA
Sbjct: 181 AGLNIVGLGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVVILVA 240

Query: 241 ENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVASFIQS 300
           ENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVASFIQS
Sbjct: 241 ENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVASFIQS 300

Query: 301 T 302
           T
Sbjct: 301 T 301

BLAST of MS020480 vs. NCBI nr
Match: KAG6605131.1 (putative carboxylesterase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 483.8 bits (1244), Expect = 1.1e-132
Identity = 239/304 (78.62%), Postives = 262/304 (86.18%), Query Frame = 0

Query: 1   MDSDAADEVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVR 60
           MDS A +EVVF+F  VFRVF+SGRVERL+P D VPPSTD RTGVQSKD+LIDP T +SVR
Sbjct: 1   MDSTAGEEVVFQFLSVFRVFKSGRVERLVPADTVPPSTDSRTGVQSKDVLIDPHTKLSVR 60

Query: 61  IYLPPVPDST-KLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEH 120
           IYLP V  S  KLPLLIYIHGGAFAIGS  S+IYH HLISLAAEANAVA+SV YRLAPE+
Sbjct: 61  IYLPKVSTSAKKLPLLIYIHGGAFAIGSVASSIYHSHLISLAAEANAVAVSVEYRLAPEN 120

Query: 121 PLPAAYDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAG-- 180
           PLPAAYDDAW +VEW ASH+NRDGAE+WLN HADFDRVF+VGDSAGANISYN+VGRA   
Sbjct: 121 PLPAAYDDAWKAVEWVASHSNRDGAEMWLNEHADFDRVFIVGDSAGANISYNVVGRASEN 180

Query: 181 -NGPAGLNIVGLGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVV 240
            NGP GL I+GLGL+HPFFMIDKPDKLIEYIFPTS GLEDPRMNP ANP L  LRC +V+
Sbjct: 181 DNGPEGLKIIGLGLVHPFFMIDKPDKLIEYIFPTSTGLEDPRMNPWANPGLGRLRCNRVL 240

Query: 241 ILVAENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVAS 300
           I+ AE+D+L DR RGFHAALRESGWKGSVE+VE EGE HVFHLFNP+ EKA  LVK VAS
Sbjct: 241 IIAAEHDFLNDRARGFHAALRESGWKGSVEMVENEGEDHVFHLFNPDCEKAVALVKTVAS 300

BLAST of MS020480 vs. NCBI nr
Match: XP_023007510.1 (probable carboxylesterase 2 [Cucurbita maxima])

HSP 1 Score: 483.4 bits (1243), Expect = 1.4e-132
Identity = 238/304 (78.29%), Postives = 262/304 (86.18%), Query Frame = 0

Query: 1   MDSDAADEVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVR 60
           MDS A +EVVF+F  VFRVF+SGRVERL+P D VPPSTD RTGVQSKD+LIDP T +SVR
Sbjct: 1   MDSTAGEEVVFQFLSVFRVFKSGRVERLVPADTVPPSTDSRTGVQSKDVLIDPHTKLSVR 60

Query: 61  IYLPPVPDST-KLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEH 120
           IYLP V  S  KLPLLIYIHGGAF IGS  S+IYH HLISLAAEANAVA+SV YRLAPE+
Sbjct: 61  IYLPKVSTSAEKLPLLIYIHGGAFTIGSVASSIYHSHLISLAAEANAVAVSVEYRLAPEN 120

Query: 121 PLPAAYDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAG-- 180
           PLPAAYDDAWT+VEW ASH+NRDGAE+WLN HADFD VF+VGDSAGANISYN+VGRA   
Sbjct: 121 PLPAAYDDAWTAVEWVASHSNRDGAEMWLNEHADFDCVFIVGDSAGANISYNVVGRASEN 180

Query: 181 -NGPAGLNIVGLGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVV 240
            NGP GLNI+GLGL+HPFFMIDKPDKLIEYIFPTS GLEDPRMNP ANP L   RC +V+
Sbjct: 181 DNGPEGLNIIGLGLVHPFFMIDKPDKLIEYIFPTSTGLEDPRMNPWANPGLARFRCNRVL 240

Query: 241 ILVAENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVAS 300
           I+ AE+D+L DR RGFHAALRESGWKGSVE+VE EGE HVFHLFNP+ EKA  LVK+VAS
Sbjct: 241 IIAAEHDFLNDRARGFHAALRESGWKGSVEMVENEGEDHVFHLFNPDCEKAVALVKKVAS 300

BLAST of MS020480 vs. NCBI nr
Match: KAG7035128.1 (putative carboxylesterase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 482.6 bits (1241), Expect = 2.4e-132
Identity = 238/304 (78.29%), Postives = 262/304 (86.18%), Query Frame = 0

Query: 1   MDSDAADEVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVR 60
           MDS A +EVVF+F  VFRVF+SGRVERL+P D VPPSTD RTGV+SKD+LIDP T +SVR
Sbjct: 1   MDSTAGEEVVFQFLSVFRVFKSGRVERLVPADTVPPSTDSRTGVESKDVLIDPHTKLSVR 60

Query: 61  IYLPPVPDST-KLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEH 120
           IYLP V  S  KLPLLIYIHGGAFAIGS  S+IYH HLISLAAEANAVA+SV YRLAPE+
Sbjct: 61  IYLPKVSTSAKKLPLLIYIHGGAFAIGSVASSIYHSHLISLAAEANAVAVSVEYRLAPEN 120

Query: 121 PLPAAYDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAG-- 180
           PLPAAYDDAW +VEW ASH+NRDGAE+WLN HADFDRVF+VGDSAGANISYN+VGRA   
Sbjct: 121 PLPAAYDDAWKAVEWVASHSNRDGAEMWLNEHADFDRVFIVGDSAGANISYNVVGRASEN 180

Query: 181 -NGPAGLNIVGLGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVV 240
            NGP GL I+GLGL+HPFFMIDKPDKLIEYIFPTS GLEDPRMNP ANP L  LRC +V+
Sbjct: 181 DNGPEGLKIIGLGLVHPFFMIDKPDKLIEYIFPTSTGLEDPRMNPWANPGLGRLRCNRVL 240

Query: 241 ILVAENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVAS 300
           I+ AE+D+L DR RGFHAALRESGWKGSVE+VE EGE HVFHLFNP+ EKA  LVK VAS
Sbjct: 241 IIAAEHDFLNDRARGFHAALRESGWKGSVEMVENEGEDHVFHLFNPDCEKAVALVKTVAS 300

BLAST of MS020480 vs. NCBI nr
Match: XP_023532126.1 (probable carboxylesterase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 481.9 bits (1239), Expect = 4.1e-132
Identity = 237/304 (77.96%), Postives = 262/304 (86.18%), Query Frame = 0

Query: 1   MDSDAADEVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVR 60
           MDS A +EVVF+F  VFRVF+SGRVERL+P D VPPSTD RTGVQSKD+LIDP T +SVR
Sbjct: 1   MDSTAGEEVVFQFLSVFRVFKSGRVERLVPADTVPPSTDSRTGVQSKDVLIDPHTKLSVR 60

Query: 61  IYLPPVPDST-KLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEH 120
           IYLP V  S  KLPLLIYIHGGAF+IGS  S+IYH HLISLAAEANAVA+SV YRLAPE+
Sbjct: 61  IYLPKVSTSAKKLPLLIYIHGGAFSIGSVASSIYHSHLISLAAEANAVAVSVEYRLAPEN 120

Query: 121 PLPAAYDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAG-- 180
           PLPAAYDDAW +VEW ASH+NRDGAE+WLN HADFDRVF+VGDSAGANISYN+VGRA   
Sbjct: 121 PLPAAYDDAWKAVEWVASHSNRDGAEMWLNEHADFDRVFIVGDSAGANISYNVVGRASEN 180

Query: 181 -NGPAGLNIVGLGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVV 240
            NGP GL I+GLGL+HPFFMIDKPDKLIEYIFPTS GLEDPRMNP ANP L   RC +V+
Sbjct: 181 DNGPEGLKIIGLGLVHPFFMIDKPDKLIEYIFPTSTGLEDPRMNPWANPGLGRFRCNRVL 240

Query: 241 ILVAENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVAS 300
           I+ AE+D+L DR RGFHAALRESGWKGSVE+VE EGE HVFHLFNP+ EKA  LVK+VAS
Sbjct: 241 IIAAEHDFLNDRARGFHAALRESGWKGSVEMVENEGEDHVFHLFNPDCEKAVALVKKVAS 300

BLAST of MS020480 vs. ExPASy Swiss-Prot
Match: Q9SX78 (Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 1.5e-65
Identity = 136/308 (44.16%), Postives = 190/308 (61.69%), Query Frame = 0

Query: 8   EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLP-PV 67
           +V  E  P   V   G VERL   +  PP  D  TGV SKDI+I+P+TG+S RIY P  +
Sbjct: 7   QVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARIYRPFSI 66

Query: 68  PDSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPAAYD 127
               K+PL++Y HGGAF I S     YH  L  +  +AN +A+SVNYRLAPEHPLP AY+
Sbjct: 67  QPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHPLPTAYE 126

Query: 128 DAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGNGPAGLNIV 187
           D+WT+++   +       E W+N++AD D +FLVGDSAGANIS++L  RA      L I 
Sbjct: 127 DSWTALKNIQAI-----NEPWINDYADLDSLFLVGDSAGANISHHLAFRAKQSDQTLKIK 186

Query: 188 GLGLIHPFFMIDKP--------------DKLIEYIFPTSAGLEDPRMNPRA--NPELRSL 247
           G+G+IHP+F   +P              D   E++ P+  G +DP +NP A  +P+L  L
Sbjct: 187 GIGMIHPYFWGTQPIGAEIKDEARKQMVDGWWEFVCPSEKGSDDPWINPFADGSPDLGGL 246

Query: 248 RCTQVVILVAENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATL 299
            C +V+I VAE D L +RG+ ++  L +S WKG VEI+ET+ + HVFH+F P+ ++A  +
Sbjct: 247 GCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFEPDCDEAMEM 306

BLAST of MS020480 vs. ExPASy Swiss-Prot
Match: I4DST8 (Tuliposide A-converting enzyme 1, chloroplastic OS=Tulipa gesneriana OX=13306 GN=TCEA1 PE=1 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.3e-64
Identity = 129/311 (41.48%), Postives = 190/311 (61.09%), Query Frame = 0

Query: 7   DEVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLPPV 66
           DE+V + +P   +++SGR+ER + G  V P+  +   V +KD++IDP TGVSVR+YLP V
Sbjct: 81  DEIVLDLKPFLIIYKSGRIERFL-GTTVIPACPE---VATKDVVIDPATGVSVRLYLPNV 140

Query: 67  PD--STKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPAA 126
            D  S KLP+L+Y HGG F I +  S  YH +L  LAA+A  + +S+NYRLAPE+PLPA+
Sbjct: 141 VDLPSKKLPVLVYFHGGGFVIENTGSPNYHNYLTLLAAKAGVLIVSINYRLAPEYPLPAS 200

Query: 127 YDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGNGPAGLN 186
           YDD      W  SH+     E WL  H DF ++ L GDSAG N+++ +  RA  G     
Sbjct: 201 YDDCMAGFNWVVSHSAGPALEPWLAQHGDFSQILLSGDSAGGNVTHYVAMRADAGV---- 260

Query: 187 IVGLGLIHPFFMIDKP--------------DKLIEYIFPTSAGLEDPRMNPRA--NPELR 246
           I G+ ++HP+F+  +P              DKL     P + GL+DP +NP A   P L 
Sbjct: 261 IEGVAIVHPYFLGSEPVGNEINDPANIEFHDKLWRLAAPDTEGLDDPLINPVAPGAPSLA 320

Query: 247 SLRCTQVVILVAENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAA 300
            L+C + V+ VA ND+L +RGR ++ AL +SGW+G  E+V+ EG GHVFHL +   + + 
Sbjct: 321 GLKCKRAVVFVAGNDFLVERGRMYYEALVKSGWRGEAELVQHEGVGHVFHLSDYSGDISV 380

BLAST of MS020480 vs. ExPASy Swiss-Prot
Match: Q9LMA7 (Probable carboxylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=CXE1 PE=2 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 1.7e-64
Identity = 127/312 (40.71%), Postives = 187/312 (59.94%), Query Frame = 0

Query: 8   EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLPP-- 67
           E+ F++ P FR+F++G +ERL+P   VPPS +   GV SKD +  P+  +S+RIYLP   
Sbjct: 4   EIAFDYSPRFRIFKNGGIERLVPETFVPPSLNPENGVVSKDAVYSPEKNLSLRIYLPQNS 63

Query: 68  --VPDSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPA 127
                  K+PLL+Y HGG F + +A S IYH  L S  +  + +A+SV YR APEHP+P 
Sbjct: 64  VYETGEKKIPLLVYFHGGGFIMETAFSPIYHTFLTSAVSATDCIAVSVEYRRAPEHPIPT 123

Query: 128 AYDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGN---GP 187
            Y+D+W +++W  +H  R G E WLN HADF +VFL GDSAGANI++++  R       P
Sbjct: 124 LYEDSWDAIQWIFTHITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVDKEKLPP 183

Query: 188 AGLNIVGLGLIHPFFM------------IDKPDKLIEYIFPTSA-GLEDPRMNPRANPEL 247
               I G+ L HP+F+            +   ++L     P S  G+EDP +N     +L
Sbjct: 184 ENFKISGMILFHPYFLSKALIEEMEVEAMRYYERLWRIASPDSGNGVEDPWINV-VGSDL 243

Query: 248 RSLRCTQVVILVAENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKA 300
             L C +V+++VA ND L   G  + A L +SGW G V+++ET+ EGHVFHL +P++E A
Sbjct: 244 TGLGCRRVLVMVAGNDVLARGGWSYVAELEKSGWIGKVKVMETKEEGHVFHLRDPDSENA 303

BLAST of MS020480 vs. ExPASy Swiss-Prot
Match: Q9SMM9 (Probable carboxylesterase 13 OS=Arabidopsis thaliana OX=3702 GN=CXE13 PE=1 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 2.2e-64
Identity = 135/323 (41.80%), Postives = 198/323 (61.30%), Query Frame = 0

Query: 8   EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLP--- 67
           E+  ++ P+  +++SGR+ERL+    VPPS++ + GV SKD++  P   +S+RIYLP   
Sbjct: 4   EIAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYLPEKA 63

Query: 68  ---PVPDSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPL 127
                  S KLPLL+Y HGG F + +A S  YH  L +  + ++ VA+SV+YR APEHP+
Sbjct: 64  ATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAPEHPI 123

Query: 128 PAAYDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGN--- 187
           P +YDD+WT+++W  SH    G+E WLN HADF +VFL GDSAGANI++++  +A     
Sbjct: 124 PTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAAKDKL 183

Query: 188 GPAGLN---IVGLGLIHPFFMIDKP--DK---------LIEYIFPTSA-----GLEDPRM 247
            P  LN   I G+ L+HP+F    P  DK          IE ++  ++     G +DP +
Sbjct: 184 SPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDGSDDPFI 243

Query: 248 N--PRANPELRSLRCTQVVILVAENDWLKDRGRGFHAALRESGWKGSV-EIVETEGEGHV 300
           N     + +L  L C +V+++VAE D L  +G G+   L +S W G V ++VET+GEGHV
Sbjct: 244 NVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETKGEGHV 303

BLAST of MS020480 vs. ExPASy Swiss-Prot
Match: Q9SMN0 (Probable carboxylesterase 12 OS=Arabidopsis thaliana OX=3702 GN=CXE12 PE=1 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 3.8e-64
Identity = 135/318 (42.45%), Postives = 192/318 (60.38%), Query Frame = 0

Query: 8   EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLP--- 67
           E+  +  P+ ++++SGR+ERL+    VPPS++ + GV SKD++      +SVRIYLP   
Sbjct: 4   EIAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRIYLPEKA 63

Query: 68  PVPDSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPAA 127
                +KLPLL+Y HGG F I +A S  YH  L +  + +N VA+SV+YR APEHP+   
Sbjct: 64  AAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHPISVP 123

Query: 128 YDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGNG--PAG 187
           +DD+WT+++W  +H    G E WLN HADF RVFL GDSAGANI +++  RA       G
Sbjct: 124 FDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKLSPG 183

Query: 188 LN---IVGLGLIHPFFM----IDKPDKLIEYI-----------FPTSA-GLEDPRMN--P 247
           LN   I G+ L+HP+F     ID+ D   E +            P S  G +DP +N   
Sbjct: 184 LNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLNVVQ 243

Query: 248 RANPELRSLRCTQVVILVAENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFN 300
             + +L  L C +V+++VAE D L  +G G+ A L +SGWKG VE+VE+EGE HVFHL  
Sbjct: 244 SESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVFHLLK 303

BLAST of MS020480 vs. ExPASy TrEMBL
Match: A0A6J1D5P6 (probable carboxylesterase 2 OS=Momordica charantia OX=3673 GN=LOC111017169 PE=3 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 1.3e-171
Identity = 301/301 (100.00%), Postives = 301/301 (100.00%), Query Frame = 0

Query: 1   MDSDAADEVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVR 60
           MDSDAADEVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVR
Sbjct: 1   MDSDAADEVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVR 60

Query: 61  IYLPPVPDSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHP 120
           IYLPPVPDSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHP
Sbjct: 61  IYLPPVPDSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHP 120

Query: 121 LPAAYDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGNGP 180
           LPAAYDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGNGP
Sbjct: 121 LPAAYDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGNGP 180

Query: 181 AGLNIVGLGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVVILVA 240
           AGLNIVGLGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVVILVA
Sbjct: 181 AGLNIVGLGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVVILVA 240

Query: 241 ENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVASFIQS 300
           ENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVASFIQS
Sbjct: 241 ENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVASFIQS 300

Query: 301 T 302
           T
Sbjct: 301 T 301

BLAST of MS020480 vs. ExPASy TrEMBL
Match: A0A6J1L361 (probable carboxylesterase 2 OS=Cucurbita maxima OX=3661 GN=LOC111499978 PE=4 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 6.8e-133
Identity = 238/304 (78.29%), Postives = 262/304 (86.18%), Query Frame = 0

Query: 1   MDSDAADEVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVR 60
           MDS A +EVVF+F  VFRVF+SGRVERL+P D VPPSTD RTGVQSKD+LIDP T +SVR
Sbjct: 1   MDSTAGEEVVFQFLSVFRVFKSGRVERLVPADTVPPSTDSRTGVQSKDVLIDPHTKLSVR 60

Query: 61  IYLPPVPDST-KLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEH 120
           IYLP V  S  KLPLLIYIHGGAF IGS  S+IYH HLISLAAEANAVA+SV YRLAPE+
Sbjct: 61  IYLPKVSTSAEKLPLLIYIHGGAFTIGSVASSIYHSHLISLAAEANAVAVSVEYRLAPEN 120

Query: 121 PLPAAYDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAG-- 180
           PLPAAYDDAWT+VEW ASH+NRDGAE+WLN HADFD VF+VGDSAGANISYN+VGRA   
Sbjct: 121 PLPAAYDDAWTAVEWVASHSNRDGAEMWLNEHADFDCVFIVGDSAGANISYNVVGRASEN 180

Query: 181 -NGPAGLNIVGLGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVV 240
            NGP GLNI+GLGL+HPFFMIDKPDKLIEYIFPTS GLEDPRMNP ANP L   RC +V+
Sbjct: 181 DNGPEGLNIIGLGLVHPFFMIDKPDKLIEYIFPTSTGLEDPRMNPWANPGLARFRCNRVL 240

Query: 241 ILVAENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVAS 300
           I+ AE+D+L DR RGFHAALRESGWKGSVE+VE EGE HVFHLFNP+ EKA  LVK+VAS
Sbjct: 241 IIAAEHDFLNDRARGFHAALRESGWKGSVEMVENEGEDHVFHLFNPDCEKAVALVKKVAS 300

BLAST of MS020480 vs. ExPASy TrEMBL
Match: A0A6J1G929 (probable carboxylesterase 2 OS=Cucurbita moschata OX=3662 GN=LOC111451823 PE=4 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 9.9e-132
Identity = 237/304 (77.96%), Postives = 260/304 (85.53%), Query Frame = 0

Query: 1   MDSDAADEVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVR 60
           MDS A +EVVF+F  VFRVF+SGRVERL+P D VPPSTD RTGVQSKD+LIDP T +SVR
Sbjct: 1   MDSTAGEEVVFQFLSVFRVFKSGRVERLVPADTVPPSTDSRTGVQSKDVLIDPHTKLSVR 60

Query: 61  IYLPPVPDST-KLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEH 120
           IYLP V  S  KLPLLIYIHGGAFAIGS  S+IYH HLISLAAEANAVA+SV YRLAPE+
Sbjct: 61  IYLPKVSTSAKKLPLLIYIHGGAFAIGSVASSIYHSHLISLAAEANAVAVSVEYRLAPEN 120

Query: 121 PLPAAYDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAG-- 180
           PLPAAYDDAW +VEW ASH+NRDGAE+WLN HADFDRVF+VGDSAGANISYN+VGRA   
Sbjct: 121 PLPAAYDDAWKAVEWVASHSNRDGAEMWLNEHADFDRVFIVGDSAGANISYNVVGRASEN 180

Query: 181 -NGPAGLNIVGLGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVV 240
            NGP GL I+GLGL+HPFFMIDKPDKLIEYIFPTS GLEDPRMNP ANP L   RC +V+
Sbjct: 181 DNGPEGLKIIGLGLVHPFFMIDKPDKLIEYIFPTSTGLEDPRMNPWANPGLGRFRCNRVL 240

Query: 241 ILVAENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVAS 300
           I+ AE+D+L DR RGFHAALRESGWKGSVE+VE EGE HVFHLFNP+  KA  LVK VAS
Sbjct: 241 IIAAEHDFLNDRARGFHAALRESGWKGSVEMVENEGEDHVFHLFNPDCGKAVALVKTVAS 300

BLAST of MS020480 vs. ExPASy TrEMBL
Match: Q0ZPW5 (CXE carboxylesterase OS=Malus pumila OX=283210 GN=CXE5 PE=2 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 3.3e-95
Identity = 169/300 (56.33%), Postives = 228/300 (76.00%), Query Frame = 0

Query: 1   MDSDAADEVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVR 60
           MDS  + EV FEF   FR++  GR ER    + VPPSTD  TGVQ KDI++ PQ+G+S R
Sbjct: 1   MDSKPSSEVSFEFPTAFRIYNDGRTERFKGIETVPPSTDSTTGVQCKDIVLSPQSGLSAR 60

Query: 61  IYLPPVPDST-KLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEH 120
           ++LP +PD T KLPLLI+IHGGAF I S  S +YH+H++ LA+EAN VALSV+YR APEH
Sbjct: 61  VFLPKLPDPTRKLPLLIFIHGGAFVIESPYSPLYHKHVMLLASEANVVALSVHYRRAPEH 120

Query: 121 PLPAAYDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAG-N 180
           PLP A++D+W +VEWAA+H+ R+G E WLN+H DFDRVF+ GDSAGA +++++V +AG +
Sbjct: 121 PLPVAFEDSWDAVEWAAAHSTRNGPEAWLNDHVDFDRVFIGGDSAGATLTHHVVRQAGLD 180

Query: 181 GPAGLNIVGLGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVVIL 240
           G +G  IVG+ L HP+FM D+PDKL+E I+PT  G +DPR+ P  +P+L  + C +V++ 
Sbjct: 181 GLSGTRIVGMILFHPYFMDDEPDKLLEVIYPTCGGSDDPRVRPGNDPKLGEIGCGRVLVF 240

Query: 241 VAENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVASFI 299
           VAE D+L+DRG  +H AL++SG+ G VEIVE++GE HVFHLFNP  + A  LVK+V SF+
Sbjct: 241 VAEKDFLRDRGWAYHEALKKSGYGGVVEIVESQGEDHVFHLFNPSCDNAVDLVKKVVSFV 300

BLAST of MS020480 vs. ExPASy TrEMBL
Match: A0A6A1V3B3 (Putative carboxylesterase 2 OS=Morella rubra OX=262757 GN=CJ030_MR7G011432 PE=4 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 1.3e-94
Identity = 171/295 (57.97%), Postives = 221/295 (74.92%), Query Frame = 0

Query: 5   AADEVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLP 64
           +A  V  +F P FRVF  G VERL+  + VPP+TD  TGVQSKD+ I  +TGVS R++LP
Sbjct: 4   SAKAVAHDFPPFFRVFEDGTVERLLGTETVPPTTDSFTGVQSKDVTISAETGVSARLFLP 63

Query: 65  PVPDST-KLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPA 124
               ST KLPLLIY+HGGAF+I S  S  YH H+ SL AEAN VALS++YR APEHPLP 
Sbjct: 64  KTASSTHKLPLLIYVHGGAFSIESPFSPAYHSHVSSLVAEANVVALSIHYRRAPEHPLPI 123

Query: 125 AYDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAG-NGPAG 184
           AYDD W +++WAA+H   +G E WLN+H DF+RVF+ GDSAGANI++ +V +AG +G  G
Sbjct: 124 AYDDTWAAMKWAATHCRCNGPEAWLNDHVDFERVFVAGDSAGANIAHVMVRKAGDDGLEG 183

Query: 185 LNIVGLGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVVILVAEN 244
           + IVG+ L+HP+F  D+PDKL++++FPT +G  D R+ P  +  L SL C +V+ILVAE 
Sbjct: 184 VKIVGMVLVHPYFGNDRPDKLLDFVFPTISGPNDHRIYPNKDSNLSSLGCNRVLILVAER 243

Query: 245 DWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVASF 298
           DWL +RGR ++ AL++SGW G+VEIVETEGE HVFHLFNP  +KA  L+KQ+ASF
Sbjct: 244 DWLNERGRTYYEALKQSGWGGTVEIVETEGEDHVFHLFNPNCDKALALLKQMASF 298

BLAST of MS020480 vs. TAIR 10
Match: AT1G47480.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 251.1 bits (640), Expect = 1.1e-66
Identity = 136/308 (44.16%), Postives = 190/308 (61.69%), Query Frame = 0

Query: 8   EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLP-PV 67
           +V  E  P   V   G VERL   +  PP  D  TGV SKDI+I+P+TG+S RIY P  +
Sbjct: 7   QVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARIYRPFSI 66

Query: 68  PDSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPAAYD 127
               K+PL++Y HGGAF I S     YH  L  +  +AN +A+SVNYRLAPEHPLP AY+
Sbjct: 67  QPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHPLPTAYE 126

Query: 128 DAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGNGPAGLNIV 187
           D+WT+++   +       E W+N++AD D +FLVGDSAGANIS++L  RA      L I 
Sbjct: 127 DSWTALKNIQAI-----NEPWINDYADLDSLFLVGDSAGANISHHLAFRAKQSDQTLKIK 186

Query: 188 GLGLIHPFFMIDKP--------------DKLIEYIFPTSAGLEDPRMNPRA--NPELRSL 247
           G+G+IHP+F   +P              D   E++ P+  G +DP +NP A  +P+L  L
Sbjct: 187 GIGMIHPYFWGTQPIGAEIKDEARKQMVDGWWEFVCPSEKGSDDPWINPFADGSPDLGGL 246

Query: 248 RCTQVVILVAENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATL 299
            C +V+I VAE D L +RG+ ++  L +S WKG VEI+ET+ + HVFH+F P+ ++A  +
Sbjct: 247 GCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFEPDCDEAMEM 306

BLAST of MS020480 vs. TAIR 10
Match: AT1G19190.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 247.7 bits (631), Expect = 1.2e-65
Identity = 127/312 (40.71%), Postives = 187/312 (59.94%), Query Frame = 0

Query: 8   EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLPP-- 67
           E+ F++ P FR+F++G +ERL+P   VPPS +   GV SKD +  P+  +S+RIYLP   
Sbjct: 4   EIAFDYSPRFRIFKNGGIERLVPETFVPPSLNPENGVVSKDAVYSPEKNLSLRIYLPQNS 63

Query: 68  --VPDSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPA 127
                  K+PLL+Y HGG F + +A S IYH  L S  +  + +A+SV YR APEHP+P 
Sbjct: 64  VYETGEKKIPLLVYFHGGGFIMETAFSPIYHTFLTSAVSATDCIAVSVEYRRAPEHPIPT 123

Query: 128 AYDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGN---GP 187
            Y+D+W +++W  +H  R G E WLN HADF +VFL GDSAGANI++++  R       P
Sbjct: 124 LYEDSWDAIQWIFTHITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVDKEKLPP 183

Query: 188 AGLNIVGLGLIHPFFM------------IDKPDKLIEYIFPTSA-GLEDPRMNPRANPEL 247
               I G+ L HP+F+            +   ++L     P S  G+EDP +N     +L
Sbjct: 184 ENFKISGMILFHPYFLSKALIEEMEVEAMRYYERLWRIASPDSGNGVEDPWINV-VGSDL 243

Query: 248 RSLRCTQVVILVAENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKA 300
             L C +V+++VA ND L   G  + A L +SGW G V+++ET+ EGHVFHL +P++E A
Sbjct: 244 TGLGCRRVLVMVAGNDVLARGGWSYVAELEKSGWIGKVKVMETKEEGHVFHLRDPDSENA 303

BLAST of MS020480 vs. TAIR 10
Match: AT3G48700.1 (carboxyesterase 13 )

HSP 1 Score: 247.3 bits (630), Expect = 1.6e-65
Identity = 135/323 (41.80%), Postives = 198/323 (61.30%), Query Frame = 0

Query: 8   EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLP--- 67
           E+  ++ P+  +++SGR+ERL+    VPPS++ + GV SKD++  P   +S+RIYLP   
Sbjct: 4   EIAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYLPEKA 63

Query: 68  ---PVPDSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPL 127
                  S KLPLL+Y HGG F + +A S  YH  L +  + ++ VA+SV+YR APEHP+
Sbjct: 64  ATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAPEHPI 123

Query: 128 PAAYDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGN--- 187
           P +YDD+WT+++W  SH    G+E WLN HADF +VFL GDSAGANI++++  +A     
Sbjct: 124 PTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAAKDKL 183

Query: 188 GPAGLN---IVGLGLIHPFFMIDKP--DK---------LIEYIFPTSA-----GLEDPRM 247
            P  LN   I G+ L+HP+F    P  DK          IE ++  ++     G +DP +
Sbjct: 184 SPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDGSDDPFI 243

Query: 248 N--PRANPELRSLRCTQVVILVAENDWLKDRGRGFHAALRESGWKGSV-EIVETEGEGHV 300
           N     + +L  L C +V+++VAE D L  +G G+   L +S W G V ++VET+GEGHV
Sbjct: 244 NVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETKGEGHV 303

BLAST of MS020480 vs. TAIR 10
Match: AT3G48690.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 246.5 bits (628), Expect = 2.7e-65
Identity = 135/318 (42.45%), Postives = 192/318 (60.38%), Query Frame = 0

Query: 8   EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLP--- 67
           E+  +  P+ ++++SGR+ERL+    VPPS++ + GV SKD++      +SVRIYLP   
Sbjct: 4   EIAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRIYLPEKA 63

Query: 68  PVPDSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPAA 127
                +KLPLL+Y HGG F I +A S  YH  L +  + +N VA+SV+YR APEHP+   
Sbjct: 64  AAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHPISVP 123

Query: 128 YDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGNG--PAG 187
           +DD+WT+++W  +H    G E WLN HADF RVFL GDSAGANI +++  RA       G
Sbjct: 124 FDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKLSPG 183

Query: 188 LN---IVGLGLIHPFFM----IDKPDKLIEYI-----------FPTSA-GLEDPRMN--P 247
           LN   I G+ L+HP+F     ID+ D   E +            P S  G +DP +N   
Sbjct: 184 LNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLNVVQ 243

Query: 248 RANPELRSLRCTQVVILVAENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFN 300
             + +L  L C +V+++VAE D L  +G G+ A L +SGWKG VE+VE+EGE HVFHL  
Sbjct: 244 SESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVFHLLK 303

BLAST of MS020480 vs. TAIR 10
Match: AT2G03550.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 243.8 bits (621), Expect = 1.8e-64
Identity = 130/307 (42.35%), Postives = 183/307 (59.61%), Query Frame = 0

Query: 9   VVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLPPVPD 68
           + F+  P+FRV++SGR+ERL+    VPPS   + GV SKDI+  P+  +S+RIYLP    
Sbjct: 5   IAFDRSPMFRVYKSGRIERLLGETTVPPSLTPQNGVVSKDIIHSPEKNLSLRIYLPEKVT 64

Query: 69  STKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPAAYDDA 128
             KLP+LIY HGG F I +A S  YH  L S  A AN +A+SVNYR APE P+P  Y+D+
Sbjct: 65  VKKLPILIYFHGGGFIIETAFSPPYHTFLTSAVAAANCLAISVNYRRAPEFPVPIPYEDS 124

Query: 129 WTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGNGPAGLNIV-G 188
           W S++W  +H    G E W+N H DF +VFL GDSAG NIS++L  RA       +++ G
Sbjct: 125 WDSLKWVLTHITGTGPETWINKHGDFGKVFLAGDSAGGNISHHLTMRAKKEKLCDSLISG 184

Query: 189 LGLIHPFFMIDKPDKLIE---------------YIFPTS-AGLEDPRMNPRANPELRSLR 248
           + LIHP+F    P    E                  P S  G++DP +N     +   L 
Sbjct: 185 IILIHPYFWSKTPIDEFEVRDVGKTKGVEGSWRVASPNSKQGVDDPWLNV-VGSDPSGLG 244

Query: 249 CTQVVILVAENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLV 299
           C +V+++VA +D    +G  +   L++SGW+G VE++ET+ EGHVFHL NP ++ A  +V
Sbjct: 245 CGRVLVMVAGDDLFVRQGWCYAEKLKKSGWEGEVEVMETKNEGHVFHLKNPNSDNARQVV 304

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022148541.12.6e-171100.00probable carboxylesterase 2 [Momordica charantia][more]
KAG6605131.11.1e-13278.62putative carboxylesterase 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023007510.11.4e-13278.29probable carboxylesterase 2 [Cucurbita maxima][more]
KAG7035128.12.4e-13278.29putative carboxylesterase 2 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023532126.14.1e-13277.96probable carboxylesterase 2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9SX781.5e-6544.16Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1[more]
I4DST81.3e-6441.48Tuliposide A-converting enzyme 1, chloroplastic OS=Tulipa gesneriana OX=13306 GN... [more]
Q9LMA71.7e-6440.71Probable carboxylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=CXE1 PE=2 SV=1[more]
Q9SMM92.2e-6441.80Probable carboxylesterase 13 OS=Arabidopsis thaliana OX=3702 GN=CXE13 PE=1 SV=1[more]
Q9SMN03.8e-6442.45Probable carboxylesterase 12 OS=Arabidopsis thaliana OX=3702 GN=CXE12 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1D5P61.3e-171100.00probable carboxylesterase 2 OS=Momordica charantia OX=3673 GN=LOC111017169 PE=3 ... [more]
A0A6J1L3616.8e-13378.29probable carboxylesterase 2 OS=Cucurbita maxima OX=3661 GN=LOC111499978 PE=4 SV=... [more]
A0A6J1G9299.9e-13277.96probable carboxylesterase 2 OS=Cucurbita moschata OX=3662 GN=LOC111451823 PE=4 S... [more]
Q0ZPW53.3e-9556.33CXE carboxylesterase OS=Malus pumila OX=283210 GN=CXE5 PE=2 SV=1[more]
A0A6A1V3B31.3e-9457.97Putative carboxylesterase 2 OS=Morella rubra OX=262757 GN=CJ030_MR7G011432 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT1G47480.11.1e-6644.16alpha/beta-Hydrolases superfamily protein [more]
AT1G19190.11.2e-6540.71alpha/beta-Hydrolases superfamily protein [more]
AT3G48700.11.6e-6541.80carboxyesterase 13 [more]
AT3G48690.12.7e-6542.45alpha/beta-Hydrolases superfamily protein [more]
AT2G03550.11.8e-6442.35alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 21..301
e-value: 2.3E-72
score: 246.1
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 18..300
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 75..278
e-value: 8.9E-46
score: 156.4
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 5..298
NoneNo IPR availablePANTHERPTHR23024:SF414CARBOXYLESTERASE 13-RELATEDcoord: 5..298
IPR002168Lipase, GDXG, putative histidine active sitePROSITEPS01173LIPASE_GDXG_HIScoord: 75..91

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS020480.1MS020480.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity