MS020331 (gene) Bitter gourd (TR) v1

Overview


Name	MS020331
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	CST complex subunit STN1
Location	scaffold665: 1090951 .. 1091457 (+)
RNA-Seq Expression	MS020331
Synteny	MS020331

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TCGGAGAATTTCCGTAGCTTTCCGATGGACCACCGCCACGTTAAGCTCCTGGGTTTTGACCTTCTTTCACTCACTCAAACTTCATCTTCTTCCTCCTCCGATGCCGTCTCCTTCTCTCGCAAAGGCCATGCCGTCTCCTGTGCAGAGATCGTCGGCGTCGTGGTCTCCCGTGACCTCAAACCCAACAGATTTCTCAAGTTTTCCGTCGACGATGGCACCGGCTGTGTTAACTGTATTCTATGGCTCAATCAGCTGAGCTCGCCTTACTTTGCAAGCCGGCGCCCACCAGATGTTCGAATAATTGCTAATATGTCGACCCATTTCGCTGCCCAAATTAGGGTTGGGATTGTTGGTAGAGTCCGGGGGAAGGTCACCAGCTATAGGGGCGTGGTGCAGATCACGGTGTCAGATGTTGTGGTTGAGGATGACCCAAATGCTCAGATTCTGCATTGGTTGGATTGCATGAGGTTGGCTCTAAAGTGTTATGACCTCTTGCCTACACCCACA

mRNA sequence

TCGGAGAATTTCCGTAGCTTTCCGATGGACCACCGCCACGTTAAGCTCCTGGGTTTTGACCTTCTTTCACTCACTCAAACTTCATCTTCTTCCTCCTCCGATGCCGTCTCCTTCTCTCGCAAAGGCCATGCCGTCTCCTGTGCAGAGATCGTCGGCGTCGTGGTCTCCCGTGACCTCAAACCCAACAGATTTCTCAAGTTTTCCGTCGACGATGGCACCGGCTGTGTTAACTGTATTCTATGGCTCAATCAGCTGAGCTCGCCTTACTTTGCAAGCCGGCGCCCACCAGATGTTCGAATAATTGCTAATATGTCGACCCATTTCGCTGCCCAAATTAGGGTTGGGATTGTTGGTAGAGTCCGGGGGAAGGTCACCAGCTATAGGGGCGTGGTGCAGATCACGGTGTCAGATGTTGTGGTTGAGGATGACCCAAATGCTCAGATTCTGCATTGGTTGGATTGCATGAGGTTGGCTCTAAAGTGTTATGACCTCTTGCCTACACCCACA

Coding sequence (CDS)

TCGGAGAATTTCCGTAGCTTTCCGATGGACCACCGCCACGTTAAGCTCCTGGGTTTTGACCTTCTTTCACTCACTCAAACTTCATCTTCTTCCTCCTCCGATGCCGTCTCCTTCTCTCGCAAAGGCCATGCCGTCTCCTGTGCAGAGATCGTCGGCGTCGTGGTCTCCCGTGACCTCAAACCCAACAGATTTCTCAAGTTTTCCGTCGACGATGGCACCGGCTGTGTTAACTGTATTCTATGGCTCAATCAGCTGAGCTCGCCTTACTTTGCAAGCCGGCGCCCACCAGATGTTCGAATAATTGCTAATATGTCGACCCATTTCGCTGCCCAAATTAGGGTTGGGATTGTTGGTAGAGTCCGGGGGAAGGTCACCAGCTATAGGGGCGTGGTGCAGATCACGGTGTCAGATGTTGTGGTTGAGGATGACCCAAATGCTCAGATTCTGCATTGGTTGGATTGCATGAGGTTGGCTCTAAAGTGTTATGACCTCTTGCCTACACCCACA

Protein sequence

SENFRSFPMDHRHVKLLGFDLLSLTQTSSSSSSDAVSFSRKGHAVSCAEIVGVVVSRDLKPNRFLKFSVDDGTGCVNCILWLNQLSSPYFASRRPPDVRIIANMSTHFAAQIRVGIVGRVRGKVTSYRGVVQITVSDVVVEDDPNAQILHWLDCMRLALKCYDLLPTPT

Homology

[Close]

BLAST of MS020331 vs. NCBI nr
Match: XP_023553655.1 (CST complex subunit STN1 [Cucurbita pepo subsp. pepo] >XP_023553656.1 CST complex subunit STN1 [Cucurbita pepo subsp. pepo] >XP_023553657.1 CST complex subunit STN1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 283.1 bits (723), Expect = 1.6e-72
Identity = 136/164 (82.93%), Postives = 150/164 (91.46%), Query Frame = 0


Query: 5   RSFPMDHRHVKLLGFDLLSLTQTSSSSSSDAVSFSRKGHAVSCAEIVGVVVSRDLKPNRF 64
           R+F + H H+KLLGFDL SL Q+SSSSSSDAVSFSR+G+AVSC E+VGVVV RDLKPNRF
Sbjct: 5   RAFSLYHTHIKLLGFDLNSLAQSSSSSSSDAVSFSRRGYAVSCVEVVGVVVFRDLKPNRF 64

Query: 65  LKFSVDDGTGCVNCILWLNQLSSPYFASRRPPDVRIIANMSTHFAAQIRVGIVGRVRGKV 124
           L+FSVDDGTGCV+CILWLN L+SPYFASR  PDVRII NM+THFAAQIRVGIV RVRGKV
Sbjct: 65  LRFSVDDGTGCVSCILWLNHLTSPYFASRHQPDVRIIGNMATHFAAQIRVGIVARVRGKV 124

Query: 125 TSYRGVVQITVSDVVVEDDPNAQILHWLDCMRLALKCYDLLPTP 169
           +SYRGVVQITVSDVVVE+DPNA+ILHWLDCMRLALKCYDL P P
Sbjct: 125 SSYRGVVQITVSDVVVENDPNAEILHWLDCMRLALKCYDLSPAP 168

[Close]

BLAST of MS020331 vs. NCBI nr
Match: XP_022963452.1 (LOW QUALITY PROTEIN: CST complex subunit STN1 [Cucurbita moschata])

HSP 1 Score: 282.0 bits (720), Expect = 3.5e-72
Identity = 135/164 (82.32%), Postives = 151/164 (92.07%), Query Frame = 0


Query: 5   RSFPMDHRHVKLLGFDLLSLTQTSSSSSSDAVSFSRKGHAVSCAEIVGVVVSRDLKPNRF 64
           R+F + H H+KLLGFDL SLTQ++SSSSSDAVSFSR+G+AVSC EIVGVVV RDLKPNRF
Sbjct: 45  RAFSLYHTHIKLLGFDLNSLTQSASSSSSDAVSFSRRGYAVSCVEIVGVVVFRDLKPNRF 104

Query: 65  LKFSVDDGTGCVNCILWLNQLSSPYFASRRPPDVRIIANMSTHFAAQIRVGIVGRVRGKV 124
           L+FSVDDGTGCV+CILWLN L+SPYFASR  PDVRII NM+THFAAQIRVGIV RVRG+V
Sbjct: 105 LRFSVDDGTGCVSCILWLNHLTSPYFASRHQPDVRIIGNMATHFAAQIRVGIVARVRGRV 164

Query: 125 TSYRGVVQITVSDVVVEDDPNAQILHWLDCMRLALKCYDLLPTP 169
           +SYRGVVQ+TVSDVVVE+DPNA+ILHWLDCMRLALKCYDL P P
Sbjct: 165 SSYRGVVQMTVSDVVVENDPNAEILHWLDCMRLALKCYDLSPAP 208

[Close]

BLAST of MS020331 vs. NCBI nr
Match: KAG6571457.1 (CST complex subunit STN1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 278.5 bits (711), Expect = 3.9e-71
Identity = 137/165 (83.03%), Postives = 150/165 (90.91%), Query Frame = 0


Query: 5   RSFPMDHRHVKLLGFDLLSLTQ-TSSSSSSDAVSFSRKGHAVSCAEIVGVVVSRDLKPNR 64
           R+F + H H+KLLGFDL SLTQ +SSSSSSDAVSFSR+G+AVSC EIVGVVV RDLKPNR
Sbjct: 24  RAFSLYHTHIKLLGFDLNSLTQSSSSSSSSDAVSFSRRGYAVSCVEIVGVVVFRDLKPNR 83

Query: 65  FLKFSVDDGTGCVNCILWLNQLSSPYFASRRPPDVRIIANMSTHFAAQIRVGIVGRVRGK 124
           FL+FSVDDGTGCV+CILWLN L+SPYFA R  PDVRII NM+THFAAQIRVGIV RVRGK
Sbjct: 84  FLRFSVDDGTGCVSCILWLNHLTSPYFALRHQPDVRIIGNMATHFAAQIRVGIVARVRGK 143

Query: 125 VTSYRGVVQITVSDVVVEDDPNAQILHWLDCMRLALKCYDLLPTP 169
           V+SYRGVVQITVSDVVVE+DPNA+ILHWLDCMRLALKCYDL P P
Sbjct: 144 VSSYRGVVQITVSDVVVENDPNAEILHWLDCMRLALKCYDLSPAP 188

[Close]

BLAST of MS020331 vs. NCBI nr
Match: KAG7011223.1 (CST complex subunit STN1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 278.5 bits (711), Expect = 3.9e-71
Identity = 137/165 (83.03%), Postives = 150/165 (90.91%), Query Frame = 0


Query: 5   RSFPMDHRHVKLLGFDLLSLTQ-TSSSSSSDAVSFSRKGHAVSCAEIVGVVVSRDLKPNR 64
           R+F + H H+KLLGFDL SLTQ +SSSSSSDAVSFSR+G+AVSC EIVGVVV RDLKPNR
Sbjct: 5   RAFSLYHTHIKLLGFDLNSLTQSSSSSSSSDAVSFSRRGYAVSCVEIVGVVVFRDLKPNR 64

Query: 65  FLKFSVDDGTGCVNCILWLNQLSSPYFASRRPPDVRIIANMSTHFAAQIRVGIVGRVRGK 124
           FL+FSVDDGTGCV+CILWLN L+SPYFA R  PDVRII NM+THFAAQIRVGIV RVRGK
Sbjct: 65  FLRFSVDDGTGCVSCILWLNHLTSPYFALRHQPDVRIIGNMATHFAAQIRVGIVARVRGK 124

Query: 125 VTSYRGVVQITVSDVVVEDDPNAQILHWLDCMRLALKCYDLLPTP 169
           V+SYRGVVQITVSDVVVE+DPNA+ILHWLDCMRLALKCYDL P P
Sbjct: 125 VSSYRGVVQITVSDVVVENDPNAEILHWLDCMRLALKCYDLSPAP 169

[Close]

BLAST of MS020331 vs. NCBI nr
Match: XP_022967373.1 (CST complex subunit STN1 [Cucurbita maxima] >XP_022967374.1 CST complex subunit STN1 [Cucurbita maxima] >XP_022967375.1 CST complex subunit STN1 [Cucurbita maxima])

HSP 1 Score: 264.6 bits (675), Expect = 5.8e-67
Identity = 129/151 (85.43%), Postives = 141/151 (93.38%), Query Frame = 0


Query: 5   RSFPMDHRHVKLLGFDLLSLTQTSSSSSSDAVSFSRKGHAVSCAEIVGVVVSRDLKPNRF 64
           R+F + H H+KLLGFDL SLTQ+SSSSSSDAVSFSR+G AVSCAEIVGVVVSRDLKPNRF
Sbjct: 23  RAFSLYHTHIKLLGFDLNSLTQSSSSSSSDAVSFSRRGFAVSCAEIVGVVVSRDLKPNRF 82

Query: 65  LKFSVDDGTGCVNCILWLNQLSSPYFASRRPPDVRIIANMSTHFAAQIRVGIVGRVRGKV 124
           L+FSVDDGTGCV+CILWLN L+SPYFASR  PDVRII NM+THFAAQIRVGIV RVRGKV
Sbjct: 83  LRFSVDDGTGCVSCILWLNHLTSPYFASRHQPDVRIIGNMATHFAAQIRVGIVARVRGKV 142

Query: 125 TSYRGVVQITVSDVVVEDDPNAQILHWLDCM 156
           +SYRGVVQITVSDVVVE+DPNA+ILHWLDCM
Sbjct: 143 SSYRGVVQITVSDVVVENDPNAEILHWLDCM 173

[Close]

BLAST of MS020331 vs. ExPASy Swiss-Prot
Match: Q9LMK5 (CST complex subunit STN1 OS=Arabidopsis thaliana OX=3702 GN=STN1 PE=1 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 9.0e-39
Identity = 83/151 (54.97%), Postives = 107/151 (70.86%), Query Frame = 0


Query: 13  HVKLLGFDLLSLTQTSSSSSSDAVSFSRKGHA-VSCAEIVGVVVSRDLKPNRFLKFSVDD 72
           H KL+  D+  LTQ+ + S+    SFS  G A VS  EIVG +VSRDL P +FLKF VDD
Sbjct: 9   HAKLVARDIQRLTQSPTESN----SFSLLGGACVSRVEIVGTIVSRDLTP-KFLKFGVDD 68

Query: 73  GTGCVNCILWLNQLSSPYFASRRPPDVRIIANMSTHFAAQIRVGIVGRVRGKVTSYRGVV 132
           GTGCV C++WLNQL+S YF+   P  + ++A+ +   AAQIR+G V RVRG+V SYRGV+
Sbjct: 69  GTGCVTCVMWLNQLTSSYFSRWDPATILLLASAARKQAAQIRIGAVARVRGRVGSYRGVM 128

Query: 133 QITVSDVVVEDDPNAQILHWLDCMRLALKCY 163
           QIT +  V E DPNA+ILHWL+C++L   CY
Sbjct: 129 QITANVAVAERDPNAEILHWLECLKLGQSCY 154

[Close]

BLAST of MS020331 vs. ExPASy Swiss-Prot
Match: D2GXY4 (CST complex subunit STN1 OS=Ailuropoda melanoleuca OX=9646 GN=STN1 PE=3 SV=2)

HSP 1 Score: 62.8 bits (151), Expect = 4.4e-09
Identity = 43/137 (31.39%), Postives = 67/137 (48.91%), Query Frame = 0


Query: 38  FSRKGHAVSCAEIVGVVVSRDLKPNRFLKFSVDDGTGCVNCILWLNQLSSPYFASRRPPD 97
           F   GH +   EI+G V+ R  K + F  + VDDGTG +NCI W  + S+    S   P 
Sbjct: 46  FFYNGHPIKQVEILGTVIGRREK-DAFYSYGVDDGTGVINCICW--KKSNNAAPSSAAPA 105

Query: 98  VRIIANMSTHF---------AAQIRVGIVGRVRGKVTSYRGVVQITVSDVVVEDDP--NA 157
           + ++ N+++             +I +G + ++RG V +YR   +I V+     DDP  N 
Sbjct: 106 LGVL-NLTSQLKKLQETIEQKTKIEIGDIVQIRGYVHTYREEREIRVTTYYKVDDPVCNI 165

Query: 158 QILHWLDCMRLALKCYD 164
           QI   L+   +  K YD
Sbjct: 166 QIARMLELPSIYRKVYD 178

[Close]

BLAST of MS020331 vs. ExPASy Swiss-Prot
Match: Q8LFJ8 (Replication protein A 32 kDa subunit B OS=Arabidopsis thaliana OX=3702 GN=RPA2B PE=2 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 6.5e-05
Identity = 35/134 (26.12%), Postives = 61/134 (45.52%), Query Frame = 0


Query: 22  LSLTQTSSSSSSDAVSFSRKGHAVSCAEIVGVVVSRDLKPNRFLKFSVDDGTGCVNCILW 81
           L+L Q SS+S++   +FS  G  +    IVG +   + +  + + F VDDGTG V+C+ W
Sbjct: 44  LTLKQLSSASTTGESNFSIDGVDIKTVVIVGRISRMENRITQ-VDFVVDDGTGWVDCVRW 103

Query: 82  LNQLSSPYFASRRPPDVRIIANMSTHFAAQIRVGIVGRVRGKVTSYRGVVQITVSDVVVE 141
            +                  A   T     +++G+  R+ G +  ++G   + V  V   
Sbjct: 104 CH------------------ARQETEEMEAVKLGMYVRLHGHLKIFQGKRSVNVFSVRPV 158

Query: 142 DDPNAQILHWLDCM 156
            D N  + H+ +CM
Sbjct: 164 TDFNEIVHHFTECM 158

[Close]

BLAST of MS020331 vs. ExPASy TrEMBL
Match: A0A6J1HFA9 (LOW QUALITY PROTEIN: CST complex subunit STN1 OS=Cucurbita moschata OX=3662 GN=LOC111463780 PE=4 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 1.7e-72
Identity = 135/164 (82.32%), Postives = 151/164 (92.07%), Query Frame = 0


Query: 5   RSFPMDHRHVKLLGFDLLSLTQTSSSSSSDAVSFSRKGHAVSCAEIVGVVVSRDLKPNRF 64
           R+F + H H+KLLGFDL SLTQ++SSSSSDAVSFSR+G+AVSC EIVGVVV RDLKPNRF
Sbjct: 45  RAFSLYHTHIKLLGFDLNSLTQSASSSSSDAVSFSRRGYAVSCVEIVGVVVFRDLKPNRF 104

Query: 65  LKFSVDDGTGCVNCILWLNQLSSPYFASRRPPDVRIIANMSTHFAAQIRVGIVGRVRGKV 124
           L+FSVDDGTGCV+CILWLN L+SPYFASR  PDVRII NM+THFAAQIRVGIV RVRG+V
Sbjct: 105 LRFSVDDGTGCVSCILWLNHLTSPYFASRHQPDVRIIGNMATHFAAQIRVGIVARVRGRV 164

Query: 125 TSYRGVVQITVSDVVVEDDPNAQILHWLDCMRLALKCYDLLPTP 169
           +SYRGVVQ+TVSDVVVE+DPNA+ILHWLDCMRLALKCYDL P P
Sbjct: 165 SSYRGVVQMTVSDVVVENDPNAEILHWLDCMRLALKCYDLSPAP 208

[Close]

BLAST of MS020331 vs. ExPASy TrEMBL
Match: A0A6J1HUX0 (CST complex subunit STN1 OS=Cucurbita maxima OX=3661 GN=LOC111466921 PE=4 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 2.8e-67
Identity = 129/151 (85.43%), Postives = 141/151 (93.38%), Query Frame = 0


Query: 5   RSFPMDHRHVKLLGFDLLSLTQTSSSSSSDAVSFSRKGHAVSCAEIVGVVVSRDLKPNRF 64
           R+F + H H+KLLGFDL SLTQ+SSSSSSDAVSFSR+G AVSCAEIVGVVVSRDLKPNRF
Sbjct: 23  RAFSLYHTHIKLLGFDLNSLTQSSSSSSSDAVSFSRRGFAVSCAEIVGVVVSRDLKPNRF 82

Query: 65  LKFSVDDGTGCVNCILWLNQLSSPYFASRRPPDVRIIANMSTHFAAQIRVGIVGRVRGKV 124
           L+FSVDDGTGCV+CILWLN L+SPYFASR  PDVRII NM+THFAAQIRVGIV RVRGKV
Sbjct: 83  LRFSVDDGTGCVSCILWLNHLTSPYFASRHQPDVRIIGNMATHFAAQIRVGIVARVRGKV 142

Query: 125 TSYRGVVQITVSDVVVEDDPNAQILHWLDCM 156
           +SYRGVVQITVSDVVVE+DPNA+ILHWLDCM
Sbjct: 143 SSYRGVVQITVSDVVVENDPNAEILHWLDCM 173

[Close]

BLAST of MS020331 vs. ExPASy TrEMBL
Match: A0A5D3E3H2 (CST complex subunit STN1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G001150 PE=4 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 6.9e-66
Identity = 130/164 (79.27%), Postives = 143/164 (87.20%), Query Frame = 0


Query: 6   SFPMDHRHVKLLGFDLLSLTQTSSSSSSDAVSFSRKGHAVSCAEIVGVVVSRDLKPNRFL 65
           SF +   HVKLLGFDL SL Q   +SSSD+VSFSRKG+AVSC EIVGVVV RDLKPNRFL
Sbjct: 8   SFSLYQTHVKLLGFDLNSLNQ---NSSSDSVSFSRKGYAVSCTEIVGVVVFRDLKPNRFL 67

Query: 66  KFSVDDGTGCVNCILWLNQLSSPYFASRRPPDVRIIANMSTHFAAQIRVGIVGRVRGKVT 125
           KFSVDDGT CV CILWLN L+S YFASR  PDVRI+A+M+THFAAQIRVGIV RVRGK++
Sbjct: 68  KFSVDDGTACVGCILWLNHLTSSYFASRHHPDVRILADMATHFAAQIRVGIVVRVRGKLS 127

Query: 126 SYRGVVQITVSDVVVEDDPNAQILHWLDCMRLALKCYDLLPTPT 170
           SYRG+VQITVSDVVVEDDPNA+ILHWLD MRLA+KCYDL P PT
Sbjct: 128 SYRGMVQITVSDVVVEDDPNAEILHWLDSMRLAMKCYDLSPIPT 168

[Close]

BLAST of MS020331 vs. ExPASy TrEMBL
Match: A0A1S3BLT5 (CST complex subunit STN1 OS=Cucumis melo OX=3656 GN=LOC103491245 PE=4 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 6.9e-66
Identity = 130/164 (79.27%), Postives = 143/164 (87.20%), Query Frame = 0


Query: 6   SFPMDHRHVKLLGFDLLSLTQTSSSSSSDAVSFSRKGHAVSCAEIVGVVVSRDLKPNRFL 65
           SF +   HVKLLGFDL SL Q   +SSSD+VSFSRKG+AVSC EIVGVVV RDLKPNRFL
Sbjct: 58  SFSLYQTHVKLLGFDLNSLNQ---NSSSDSVSFSRKGYAVSCTEIVGVVVFRDLKPNRFL 117

Query: 66  KFSVDDGTGCVNCILWLNQLSSPYFASRRPPDVRIIANMSTHFAAQIRVGIVGRVRGKVT 125
           KFSVDDGT CV CILWLN L+S YFASR  PDVRI+A+M+THFAAQIRVGIV RVRGK++
Sbjct: 118 KFSVDDGTACVGCILWLNHLTSSYFASRHHPDVRILADMATHFAAQIRVGIVVRVRGKLS 177

Query: 126 SYRGVVQITVSDVVVEDDPNAQILHWLDCMRLALKCYDLLPTPT 170
           SYRG+VQITVSDVVVEDDPNA+ILHWLD MRLA+KCYDL P PT
Sbjct: 178 SYRGMVQITVSDVVVEDDPNAEILHWLDSMRLAMKCYDLSPIPT 218

[Close]

BLAST of MS020331 vs. ExPASy TrEMBL
Match: A0A0A0LNV0 (OB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G223720 PE=4 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 5.8e-65
Identity = 129/164 (78.66%), Postives = 141/164 (85.98%), Query Frame = 0


Query: 6   SFPMDHRHVKLLGFDLLSLTQTSSSSSSDAVSFSRKGHAVSCAEIVGVVVSRDLKPNRFL 65
           SF +   HVKLL FDL SL QT   SSSD+VSFSRKG+AVSC EIVGVVV RDLKPNRFL
Sbjct: 8   SFSLYQTHVKLLAFDLNSLNQT---SSSDSVSFSRKGYAVSCTEIVGVVVFRDLKPNRFL 67

Query: 66  KFSVDDGTGCVNCILWLNQLSSPYFASRRPPDVRIIANMSTHFAAQIRVGIVGRVRGKVT 125
           KFSVDDGT CV CILWLN L+S YFASR   DVRI+ +M+THFAAQIRVGIV RVRGK++
Sbjct: 68  KFSVDDGTACVGCILWLNHLTSSYFASRHHQDVRILGDMATHFAAQIRVGIVVRVRGKLS 127

Query: 126 SYRGVVQITVSDVVVEDDPNAQILHWLDCMRLALKCYDLLPTPT 170
           SYRG+VQITVSDVVVEDDPNA+ILHWLD MRLA+KCYDL PTPT
Sbjct: 128 SYRGMVQITVSDVVVEDDPNAEILHWLDSMRLAMKCYDLSPTPT 168

[Close]

BLAST of MS020331 vs. TAIR 10
Match: AT1G07130.1 (Nucleic acid-binding, OB-fold-like protein )

HSP 1 Score: 161.4 bits (407), Expect = 6.4e-40
Identity = 83/151 (54.97%), Postives = 107/151 (70.86%), Query Frame = 0


Query: 13  HVKLLGFDLLSLTQTSSSSSSDAVSFSRKGHA-VSCAEIVGVVVSRDLKPNRFLKFSVDD 72
           H KL+  D+  LTQ+ + S+    SFS  G A VS  EIVG +VSRDL P +FLKF VDD
Sbjct: 9   HAKLVARDIQRLTQSPTESN----SFSLLGGACVSRVEIVGTIVSRDLTP-KFLKFGVDD 68

Query: 73  GTGCVNCILWLNQLSSPYFASRRPPDVRIIANMSTHFAAQIRVGIVGRVRGKVTSYRGVV 132
           GTGCV C++WLNQL+S YF+   P  + ++A+ +   AAQIR+G V RVRG+V SYRGV+
Sbjct: 69  GTGCVTCVMWLNQLTSSYFSRWDPATILLLASAARKQAAQIRIGAVARVRGRVGSYRGVM 128

Query: 133 QITVSDVVVEDDPNAQILHWLDCMRLALKCY 163
           QIT +  V E DPNA+ILHWL+C++L   CY
Sbjct: 129 QITANVAVAERDPNAEILHWLECLKLGQSCY 154

[Close]

BLAST of MS020331 vs. TAIR 10
Match: AT3G02920.1 (Replication protein A, subunit RPA32 )

HSP 1 Score: 48.9 bits (115), Expect = 4.6e-06
Identity = 35/134 (26.12%), Postives = 61/134 (45.52%), Query Frame = 0


Query: 22  LSLTQTSSSSSSDAVSFSRKGHAVSCAEIVGVVVSRDLKPNRFLKFSVDDGTGCVNCILW 81
           L+L Q SS+S++   +FS  G  +    IVG +   + +  + + F VDDGTG V+C+ W
Sbjct: 44  LTLKQLSSASTTGESNFSIDGVDIKTVVIVGRISRMENRITQ-VDFVVDDGTGWVDCVRW 103

Query: 82  LNQLSSPYFASRRPPDVRIIANMSTHFAAQIRVGIVGRVRGKVTSYRGVVQITVSDVVVE 141
            +                  A   T     +++G+  R+ G +  ++G   + V  V   
Sbjct: 104 CH------------------ARQETEEMEAVKLGMYVRLHGHLKIFQGKRSVNVFSVRPV 158

Query: 142 DDPNAQILHWLDCM 156
            D N  + H+ +CM
Sbjct: 164 TDFNEIVHHFTECM 158

[Close]

BLAST of MS020331 vs. TAIR 10
Match: AT3G02920.2 (Replication protein A, subunit RPA32 )

HSP 1 Score: 48.9 bits (115), Expect = 4.6e-06
Identity = 35/134 (26.12%), Postives = 61/134 (45.52%), Query Frame = 0


Query: 22  LSLTQTSSSSSSDAVSFSRKGHAVSCAEIVGVVVSRDLKPNRFLKFSVDDGTGCVNCILW 81
           L+L Q SS+S++   +FS  G  +    IVG +   + +  + + F VDDGTG V+C+ W
Sbjct: 64  LTLKQLSSASTTGESNFSIDGVDIKTVVIVGRISRMENRITQ-VDFVVDDGTGWVDCVRW 123

Query: 82  LNQLSSPYFASRRPPDVRIIANMSTHFAAQIRVGIVGRVRGKVTSYRGVVQITVSDVVVE 141
            +                  A   T     +++G+  R+ G +  ++G   + V  V   
Sbjct: 124 CH------------------ARQETEEMEAVKLGMYVRLHGHLKIFQGKRSVNVFSVRPV 178

Query: 142 DDPNAQILHWLDCM 156
            D N  + H+ +CM
Sbjct: 184 TDFNEIVHHFTECM 178

[Close]

BLAST of MS020331 vs. TAIR 10
Match: AT2G24490.1 (replicon protein A2 )

HSP 1 Score: 43.1 bits (100), Expect = 2.5e-04
Identity = 29/125 (23.20%), Postives = 57/125 (45.60%), Query Frame = 0


Query: 31  SSSDAVSFSRKGHAVSCAEIVGVVVSRDLKPNRFLKFSVDDGTGCVNCILWLNQLSSPYF 90
           SS +       G +++   +VG+V  +D      ++F++DDGTG ++C  W+++     F
Sbjct: 56  SSGEKSGLVINGISLTNVSLVGLVCDKDESKVTEVRFTLDDGTGRIDCKRWVSET----F 115

Query: 91  ASRRPPDVRIIANMSTHFAAQIRVGIVGRVRGKVTSYRGVVQITVSDVVVEDDPNAQILH 150
            +R                  +R G   R+ G + +++G  Q+ V  V    D N    H
Sbjct: 116 DARE--------------MESVRDGTYVRLSGHLKTFQGKTQLLVFSVRPIMDFNEVTFH 162

Query: 151 WLDCM 156
           +++C+
Sbjct: 176 YIECI 162

[Close]

BLAST of MS020331 vs. TAIR 10
Match: AT2G24490.2 (replicon protein A2 )

HSP 1 Score: 43.1 bits (100), Expect = 2.5e-04
Identity = 29/125 (23.20%), Postives = 57/125 (45.60%), Query Frame = 0


Query: 31  SSSDAVSFSRKGHAVSCAEIVGVVVSRDLKPNRFLKFSVDDGTGCVNCILWLNQLSSPYF 90
           SS +       G +++   +VG+V  +D      ++F++DDGTG ++C  W+++     F
Sbjct: 56  SSGEKSGLVINGISLTNVSLVGLVCDKDESKVTEVRFTLDDGTGRIDCKRWVSET----F 115

Query: 91  ASRRPPDVRIIANMSTHFAAQIRVGIVGRVRGKVTSYRGVVQITVSDVVVEDDPNAQILH 150
            +R                  +R G   R+ G + +++G  Q+ V  V    D N    H
Sbjct: 116 DARE--------------MESVRDGTYVRLSGHLKTFQGKTQLLVFSVRPIMDFNEVTFH 162

Query: 151 WLDCM 156
           +++C+
Sbjct: 176 YIECI 162

The following BLAST results are available for this feature:

BLAST of MS020331 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023553655.1	1.6e-72	82.93	CST complex subunit STN1 [Cucurbita pepo subsp. pepo] >XP_023553656.1 CST comple...	[more]
XP_022963452.1	3.5e-72	82.32	LOW QUALITY PROTEIN: CST complex subunit STN1 [Cucurbita moschata]	[more]
KAG6571457.1	3.9e-71	83.03	CST complex subunit STN1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7011223.1	3.9e-71	83.03	CST complex subunit STN1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022967373.1	5.8e-67	85.43	CST complex subunit STN1 [Cucurbita maxima] >XP_022967374.1 CST complex subunit ...	[more]

BLAST of MS020331 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LMK5	9.0e-39	54.97	CST complex subunit STN1 OS=Arabidopsis thaliana OX=3702 GN=STN1 PE=1 SV=1	[more]
D2GXY4	4.4e-09	31.39	CST complex subunit STN1 OS=Ailuropoda melanoleuca OX=9646 GN=STN1 PE=3 SV=2	[more]
Q8LFJ8	6.5e-05	26.12	Replication protein A 32 kDa subunit B OS=Arabidopsis thaliana OX=3702 GN=RPA2B ...	[more]

BLAST of MS020331 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HFA9	1.7e-72	82.32	LOW QUALITY PROTEIN: CST complex subunit STN1 OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A6J1HUX0	2.8e-67	85.43	CST complex subunit STN1 OS=Cucurbita maxima OX=3661 GN=LOC111466921 PE=4 SV=1	[more]
A0A5D3E3H2	6.9e-66	79.27	CST complex subunit STN1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A1S3BLT5	6.9e-66	79.27	CST complex subunit STN1 OS=Cucumis melo OX=3656 GN=LOC103491245 PE=4 SV=1	[more]
A0A0A0LNV0	5.8e-65	78.66	OB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G223720 PE=4 SV=...	[more]

BLAST of MS020331 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G07130.1	6.4e-40	54.97	Nucleic acid-binding, OB-fold-like protein	[more]
AT3G02920.1	4.6e-06	26.12	Replication protein A, subunit RPA32	[more]
AT3G02920.2	4.6e-06	26.12	Replication protein A, subunit RPA32	[more]
AT2G24490.1	2.5e-04	23.20	replicon protein A2	[more]
AT2G24490.2	2.5e-04	23.20	replicon protein A2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004365	OB-fold nucleic acid binding domain, AA-tRNA synthetase-type	PFAM	PF01336	tRNA_anti-codon	coord: 50..139 e-value: 8.6E-9 score: 35.3
None	No IPR available	GENE3D	2.40.50.140		coord: 8..165 e-value: 4.7E-27 score: 96.5
None	No IPR available	PANTHER	PTHR13989:SF33	CST COMPLEX SUBUNIT STN1	coord: 12..164
IPR040260	Replication factor A protein-like	PANTHER	PTHR13989	REPLICATION PROTEIN A-RELATED	coord: 12..164
IPR012340	Nucleic acid-binding, OB-fold	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 38..160

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS020331.1	MS020331.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding