MS020311 (gene) Bitter gourd (TR) v1

Overview
NameMS020311
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionMagnesium transporter
Locationscaffold665: 918060 .. 922681 (-)
RNA-Seq ExpressionMS020311
SyntenyMS020311
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGGAGATGCGTTTCTGATCCCGGTGGAATCTCAGGCTTCGGTGAAGAAGAAGACGAAGAAGAAGACTGTAATTTCCAGGAGCTGGGTTTCGCTGGACCATGAAGGGAGGAGTACTGTTTTGGACGTCGATAAGTATGCGATTATGCAAAGGGTTCAGATTCATGCCAGAGATCTTCGGCTTCTTGATCCTCTCTTATCGTATCCTTCCACTATCTTGGGCAGAGAAAGAGTGATTGTTCTCAATTTGGAGGTAAAATTGTAAAATTCTGCTAATGTAACTTCCTTGAGTTACATCATTAATTTGTTTTTGTTGCTTCACTAATTTGTTGATTTGGTCCAGCACATCAAGTCAATTATCACGGCAGACGAGGTAATAGTTTTTTTATCCTTTTCTTGACTAATTAATTGCTATTGTTTAAAATTCTCTTTAATTTCTTGGGTTTCTTTAGGCTAATTGGTTTTCATCTGTTTATTTGATGATGGGGGATTTTTTTTTTTTTTTTTTTCCAAATTCCNNNNNNAAGTCCTATTTGGGATTAATAGAGTCCCTGAAAGTGTTTCAAGTTGGCCATCTTAATGGAAGTATTTGCGTGTCAATACTGTTTACAGGATGCACATTGACAAATATGCTACGTTCGACAAACCAAAATCTAAGAGTGGAAAACTAAAATAAGTATTGACCCTTAGTAAAAAATACCATATATAGTAGATATAGAGCTTTATTTATCTGTACCATATGATTCTTCAATAGGGGGCAATTCGCCCTCTTCACTTCGCAGTTTGCAGGATCAACTTTTACAATGTTCAGTTTTTAATTATTTGTCTGTAAATTTTGAGGATGATTAATTTATTATTATTATTATTATTACTATTATTTTCTGTTAGGTGCTGCTTAGAGATCCAATGGATGAAAATGTAGTTCCAATCGTTGAAGAACTGCAGAGACGGTTGCCTTCTACAAGTTCCATTTACCAAGGACAAGGTGAAGAAGAAGAGCCCCCTACCACCCAGAATGAGTTGGCTGAAAATGGTACAGCTTGGTGAAATATTGTCTTTTTGCATTTTCCCCTCGTCTTCAATGATGTCATTTTGTTTTTGGAACTGATAATTATTGCTTGTAATATTCGAAATATTGTTCATAGTAGCTTAAGTATTTCTTCTTCTTCTTCTTCTTCTTCTTTTTTTTTTTTTTTTTTGCTTCTAAGTTTTGTCATTTGATGCAGAGTTTCCATTTGAGTTCCGTGCCTTGGAGGTTGCACTAGAAGCAATTTGTAGTTTTCTTGATGCACGTACAAGAGAACTAGAGACTGATACATATCCTGCTTTAGATGAACTGACCTCCAAGGTGAAACTTGTTTGGTTGATTTGTTCTAGCAATTGGATCCTCTTAAACATTAGTTATATCAATGAAACAGAGATAAGCTTTCATTGATATGTTCTGAACTGTCTTCTTTCCTTTACTAGTTAAAGAGCATCGTCGCATGTTTTTGTTGAATTTCTGTCATTTAACTTCCTCCTGCAGCTCAAGTTCTCAAGGGTTTATGATTAATTGGAACTGGTCAGTTTATTAGTGGATTAGATGCACCGAAGTGTTCAGCTTAAATTTATTTCCATCAAAAGAAATACAACCTTTTCATCGAGATAATTATCTTCAAGGTGCTTTTATGTGTATAGTGGCTCGTGTTAGATATCCAATAAATGAATTAAATTTACCAAACCTATCAGCTTAAGCTTTTGGGTTGAGTGGTGGTTTATTCATCCTTAACAGTTCGTACCATATTCGTACCATATTTACTTTCTAGTAAAAACTTGAAATGTGGATAGGATATCAGATTCTGAAAAAATGTTTATGATTGATTTCTCTTTTAGCAAGCTAGTGATGGAAAGAGTGTAGAAAGAGTGACATGATTGTATTCTCTTTCATTATTCTACTTTAGTTTTATTATTATGGATGGGACATGTGAAATGGACAAGTCATACATGTAATCAGAGCCAGTGCAAACTTTTGGTTATAAACCTTATCAAAGTGAGAAATGCTACATTCATAATTATGATGCTGAAAAATCCAACTAAAGAGGTTCATATATCCTTCTTGGCGAATAATCGGTCTTATGATGTTCTTGTAACACTTAAAATATATTATAAAATGGTTTTCCTTCAAATGAACTGATACAAATGTTTACTACCTCCATCTAGAGTTTGATCTGAATTAGGAACCTAATCAATTAATATGTTTACCTGATTTGCAATTAGTCCCCCTTCTTTCTAATCCAAGAAATTTGGCATTTATATGCAGATTAGCAGTCGTAACTTGGACCGAGTGCGCAAACTGAAGAGTGCAATGACAAGGTTGACCAATCGGGTGCAAAAGGTAGTCAATTTTCCGTGGCATCTATAATATGCAGTATGAGGTTATGAGCCCTACTTTACTTTCACTGCTCTCGTGTCTTCAACTGATATACCCTCCAAATACTACAACTTAAGGAGATACAAACTAACAGCCAAAAGAAGTTGCATTGCACTAGCTTCGACCAGATTTGGAATTGTTCTACTCTAGATGGTATATAGGTTCAACATCATGGTTGCTTCTTGTTTATGAGTTCGAATATGACATTCAATTTTCAGTAGAAGAAGACAACTTCATAAAATAGAGTAGTTTTTTATTACACTTTTTTTTTTTTGAGTGTTCATTATTCTGAATCTTCAATTCAAACTGTTTGGTTTACAAGTCCTTGCTACCCAAGCTATATTAATTCGATTTTGAGGATTTTGTGAGAGTCCTAATGGGGATAGTTTGGTAATACCTATTACTATATTAGTTAGGGGTGTTATGGTAATTAGTTAGGGTTGAGTTTATTATAAATAGAGGTAGTAGGGATAGGGGGTATCTTTTGGGAAATAGTTTATGGCGGAGAACTTAACATAGTTCTTTGGGAGAGAGATAGCCCTCTCGACAGGCTTTTGATATTGTAACTATCTCTGTAATATTGCAATATAGTTATCTAGTTTTGTTTAGTGTATTCAGGATCCTAAAAAAAAATTTTTCTCGATATTTACTTCTAAAACATTCAGTAAACGACCAGCTTGTAACTCACCTTTCTCCTTGCAAGTAATATCCGATTGCCACATTTGTAAATATTATCTTAGTTTAATAGAGACAAAATGAGCTAAAAGGAAACACAGTAACTGATCATTCTTGACATAAATATAAGCCAACTAGTCCCCTCAAGAACCACACAAGTGCCAAAAATCCACTTGGAAGATTCAATTCTTGGATTGGGCGGGTTAATCTATCTCCTATTGTTGCTTGCCAAATGAACAGAATATGTAAAGATACCAATAGTGGGCAACTGATATATTTTCCTCCTGCAATTTCTTTAGCTTCTTGGTGATGATGTTATATTATTCTCGATCCAGGTTAGGGATGAACTTGAACAACTTCTGGATGATGACGACGATATGGCGGAACTTTACTTGTCACGAAAAGTAGCCGGAATGTACACCCCTGAAAGTGGCTCCGGGACTCCAAATTGGTTACTTGCTTCTCCTAAAGACTATTATTCAAAGATGTCAAAAACCAGTAGAGCAAGTTTAGCTACAATCCGAGGAGAAAACGATGTTGAGGAACTTGAAATGCTACTCGAGGCATCATCGTTGTCCTTTAACATGATATAACATCTAACGTTTTGAGATTTTTGTAAATTAAGATCTAATCGAGCTTCATACTGAATGTCCTGCAGGCTTATTTTATGCAGATAGAAAGTACATTAAACAAATTAATCACGGTATGAATCAAACTTCAAGCGTGTGTTAACTGTAATTGCTATTCTTATATGATATTTTGCATACGGCATATGAAATACAACTTTCTGATTATGTGGGATTTTTGTGTATTTTGAAATTATCTTCACTTACCAGCTTCGAGAGTACATCGATGACACAGAGGATTACATCAACATTCAGGTAACCTTTCCAACATTGCTGACTACTTTTTAAGACTGTCCCATGCCATTAAAGCTACTTTTTTTTTGTGATTTTTACTTACCGATGTTGAAAATTTAAAATCTACTCATGTCTCTGAGTCAAATGCTGTGTCATCTGTTGCAGCTTGACAACCACAGGAATCAACTTATTCAGGTACTATTTCCAAGGCCTAAATCAATCTAATTCCATTTAATTTTCTGTCTCTTCACCTAAAACCTTGGATCTATTAGCTATTGAAGTGGCCAGATTGTTCACTTGATTGATATCTGACCACAAAAAATTCACGTGCAAGAACTGTATCATGTCTCTTCAATTCTCAGAAATGTAATGTTCTATGTGGCAGCTAGAACTCTTTTTGAGCTCTGGGACTGTTTGTTTGTCTATTTATTCTTTAGTGACTGCAATATTTGGAATGAATATCCCGTATACCTGGAAGGAAGATCATGAACACGTATTCAAATGGGTATGAAAATCATATCAGAAACTCTAATTCTTTTGAAACTGTCTTCTGAAGATGCAGTGTTGATGTTTTCATTTCCATGAATTTAGGTGGTCATCGTCACAGGAATAGTTTGTGCATCCATTTACTTGACGCTAAGTTCGTATGCACGGTACAAAGGTCTCGTTGAATCT

mRNA sequence

ATGGCTGGAGATGCGTTTCTGATCCCGGTGGAATCTCAGGCTTCGGTGAAGAAGAAGACGAAGAAGAAGACTGTAATTTCCAGGAGCTGGGTTTCGCTGGACCATGAAGGGAGGAGTACTGTTTTGGACGTCGATAAGTATGCGATTATGCAAAGGGTTCAGATTCATGCCAGAGATCTTCGGCTTCTTGATCCTCTCTTATCGTATCCTTCCACTATCTTGGGCAGAGAAAGAGTGATTGTTCTCAATTTGGAGCACATCAAGTCAATTATCACGGCAGACGAGGTGCTGCTTAGAGATCCAATGGATGAAAATGTAGTTCCAATCGTTGAAGAACTGCAGAGACGGTTGCCTTCTACAAGTTCCATTTACCAAGGACAAGGTGAAGAAGAAGAGCCCCCTACCACCCAGAATGAGTTGGCTGAAAATGAGTTTCCATTTGAGTTCCGTGCCTTGGAGGTTGCACTAGAAGCAATTTGTAGTTTTCTTGATGCACGTACAAGAGAACTAGAGACTGATACATATCCTGCTTTAGATGAACTGACCTCCAAGATTAGCAGTCGTAACTTGGACCGAGTGCGCAAACTGAAGAGTGCAATGACAAGGTTGACCAATCGGGTGCAAAAGGTAGTTAGGGATGAACTTGAACAACTTCTGGATGATGACGACGATATGGCGGAACTTTACTTGTCACGAAAAGTAGCCGGAATGTACACCCCTGAAAGTGGCTCCGGGACTCCAAATTGGTTACTTGCTTCTCCTAAAGACTATTATTCAAAGATGTCAAAAACCAGTAGAGCAAGTTTAGCTACAATCCGAGGAGAAAACGATGTTGAGGAACTTGAAATGCTACTCGAGGCATCATCCTTCATACTGAATGTCCTGCAGGCTTATTTTATGCAGATAGAAAGTACATTAAACAAATTAATCACGCTTCGAGAGTACATCGATGACACAGAGGATTACATCAACATTCAGCTTGACAACCACAGGAATCAACTTATTCAGCTAGAACTCTTTTTGAGCTCTGGGACTGTTTGTTTGTCTATTTATTCTTTAGTGACTGCAATATTTGGAATGAATATCCCGTATACCTGGAAGGAAGATCATGAACACGTATTCAAATGGGTGGTCATCGTCACAGGAATAGTTTGTGCATCCATTTACTTGACGCTAAGTTCGTATGCACGGTACAAAGGTCTCGTTGAATCT

Coding sequence (CDS)

ATGGCTGGAGATGCGTTTCTGATCCCGGTGGAATCTCAGGCTTCGGTGAAGAAGAAGACGAAGAAGAAGACTGTAATTTCCAGGAGCTGGGTTTCGCTGGACCATGAAGGGAGGAGTACTGTTTTGGACGTCGATAAGTATGCGATTATGCAAAGGGTTCAGATTCATGCCAGAGATCTTCGGCTTCTTGATCCTCTCTTATCGTATCCTTCCACTATCTTGGGCAGAGAAAGAGTGATTGTTCTCAATTTGGAGCACATCAAGTCAATTATCACGGCAGACGAGGTGCTGCTTAGAGATCCAATGGATGAAAATGTAGTTCCAATCGTTGAAGAACTGCAGAGACGGTTGCCTTCTACAAGTTCCATTTACCAAGGACAAGGTGAAGAAGAAGAGCCCCCTACCACCCAGAATGAGTTGGCTGAAAATGAGTTTCCATTTGAGTTCCGTGCCTTGGAGGTTGCACTAGAAGCAATTTGTAGTTTTCTTGATGCACGTACAAGAGAACTAGAGACTGATACATATCCTGCTTTAGATGAACTGACCTCCAAGATTAGCAGTCGTAACTTGGACCGAGTGCGCAAACTGAAGAGTGCAATGACAAGGTTGACCAATCGGGTGCAAAAGGTAGTTAGGGATGAACTTGAACAACTTCTGGATGATGACGACGATATGGCGGAACTTTACTTGTCACGAAAAGTAGCCGGAATGTACACCCCTGAAAGTGGCTCCGGGACTCCAAATTGGTTACTTGCTTCTCCTAAAGACTATTATTCAAAGATGTCAAAAACCAGTAGAGCAAGTTTAGCTACAATCCGAGGAGAAAACGATGTTGAGGAACTTGAAATGCTACTCGAGGCATCATCCTTCATACTGAATGTCCTGCAGGCTTATTTTATGCAGATAGAAAGTACATTAAACAAATTAATCACGCTTCGAGAGTACATCGATGACACAGAGGATTACATCAACATTCAGCTTGACAACCACAGGAATCAACTTATTCAGCTAGAACTCTTTTTGAGCTCTGGGACTGTTTGTTTGTCTATTTATTCTTTAGTGACTGCAATATTTGGAATGAATATCCCGTATACCTGGAAGGAAGATCATGAACACGTATTCAAATGGGTGGTCATCGTCACAGGAATAGTTTGTGCATCCATTTACTTGACGCTAAGTTCGTATGCACGGTACAAAGGTCTCGTTGAATCT

Protein sequence

MAGDAFLIPVESQASVKKKTKKKTVISRSWVSLDHEGRSTVLDVDKYAIMQRVQIHARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEELQRRLPSTSSIYQGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVVRDELEQLLDDDDDMAELYLSRKVAGMYTPESGSGTPNWLLASPKDYYSKMSKTSRASLATIRGENDVEELEMLLEASSFILNVLQAYFMQIESTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPYTWKEDHEHVFKWVVIVTGIVCASIYLTLSSYARYKGLVES
Homology
BLAST of MS020311 vs. NCBI nr
Match: XP_022146007.1 (magnesium transporter MRS2-I-like [Momordica charantia])

HSP 1 Score: 742.7 bits (1916), Expect = 1.7e-210
Identity = 393/404 (97.28%), Postives = 393/404 (97.28%), Query Frame = 0

Query: 1   MAGDAFLIPVESQASVKKKTKKKTVISRSWVSLDHEGRSTVLDVDKYAIMQRVQIHARDL 60
           MAGDAFLIPVESQASVKKKTKKKTVISRSWVSLDHEGRSTVLDVDKYAIMQRVQIHARDL
Sbjct: 1   MAGDAFLIPVESQASVKKKTKKKTVISRSWVSLDHEGRSTVLDVDKYAIMQRVQIHARDL 60

Query: 61  RLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEELQRRLPST 120
           RLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEELQRRLPST
Sbjct: 61  RLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEELQRRLPST 120

Query: 121 SSIYQGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETDTYPALDE 180
           SSIYQGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETDTYPALDE
Sbjct: 121 SSIYQGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETDTYPALDE 180

Query: 181 LTSKISSRNLDRVRKLKSAMTRLTNRVQKVVRDELEQLLDDDDDMAELYLSRKVAGMYTP 240
           LTSKISSRNLDRVRKLKSAMTRLTNRVQK VRDELEQLLDDDDDMAELYLSRKVAGMYTP
Sbjct: 181 LTSKISSRNLDRVRKLKSAMTRLTNRVQK-VRDELEQLLDDDDDMAELYLSRKVAGMYTP 240

Query: 241 ESGSGTPNWLLASPKDYYSKMSKTSRASLATIRGENDVEELEMLLEASSFILNVLQAYFM 300
           ESGSGTPNWLLASPKDYYSKMSKTSRASLATIRGENDVEELEMLLE          AYFM
Sbjct: 241 ESGSGTPNWLLASPKDYYSKMSKTSRASLATIRGENDVEELEMLLE----------AYFM 300

Query: 301 QIESTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGM 360
           QIESTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGM
Sbjct: 301 QIESTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGM 360

Query: 361 NIPYTWKEDHEHVFKWVVIVTGIVCASIYLTLSSYARYKGLVES 405
           NIPYTWKEDHEHVFKWVVIVTGIVCASIYLTLSSYARYKGLVES
Sbjct: 361 NIPYTWKEDHEHVFKWVVIVTGIVCASIYLTLSSYARYKGLVES 393

BLAST of MS020311 vs. NCBI nr
Match: KAG6571485.1 (Magnesium transporter MRS2-7, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011247.1 Magnesium transporter MRS2-7 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 670.2 bits (1728), Expect = 1.1e-188
Identity = 367/414 (88.65%), Postives = 376/414 (90.82%), Query Frame = 0

Query: 1   MAGDAFLIPVESQA-SVKKKT---------KKKTVISRSWVSLDHEGRSTVLDVDKYAIM 60
           MAGD F  PVESQA SVKKKT         KK TV+SRSWVSLDHEGR+TVLDVDKYAIM
Sbjct: 1   MAGDGFGFPVESQAVSVKKKTVATTTTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAIM 60

Query: 61  QRVQIHARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120
           QRVQI+ARDLRLLDPLLSYPSTILGRE VIVLNLEHIKSIITADEVLLRDPMDENVVPIV
Sbjct: 61  QRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120

Query: 121 EELQRRLPSTSSIYQGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTREL 180
           EELQRRLPST+S+YQGQGEEEEP TTQNELAENEFPFEFRALEVALEAICSFLDARTREL
Sbjct: 121 EELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTREL 180

Query: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVVRDELEQLLDDDDDMAELYL 240
           ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQK VRDELEQLLDDDDDMAELYL
Sbjct: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQK-VRDELEQLLDDDDDMAELYL 240

Query: 241 SRKVAGMYTPESGSGTPNWLLASPKDYYSKMSKTSRASLATIRGENDVEELEMLLEASSF 300
           SRKVAG  TPESGSGTP W LASPKD YSKMSKTSRAS  T RGENDVEELEMLLE    
Sbjct: 241 SRKVAG--TPESGSGTPIWFLASPKD-YSKMSKTSRASAITFRGENDVEELEMLLE---- 300

Query: 301 ILNVLQAYFMQIESTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSI 360
                 AYFMQIE TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSI
Sbjct: 301 ------AYFMQIEGTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSI 360

Query: 361 YSLVTAIFGMNIPYTWKEDHEHVFKWVVIVTGIVCASIYLTLSSYARYKGLVES 405
           YSLVTAIFGMNIPYTWKEDHEHVFKWVVI+TGIVCA+IY++LSSYAR KGLV S
Sbjct: 361 YSLVTAIFGMNIPYTWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 400

BLAST of MS020311 vs. NCBI nr
Match: XP_023554091.1 (magnesium transporter MRS2-I-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 669.1 bits (1725), Expect = 2.4e-188
Identity = 366/415 (88.19%), Postives = 376/415 (90.60%), Query Frame = 0

Query: 1   MAGDAFLIPVESQA-SVKKKT----------KKKTVISRSWVSLDHEGRSTVLDVDKYAI 60
           MAGD F  PVESQA SVKKKT          KK TV+SRSWVSLDHEGR+TVLDVDKYA+
Sbjct: 1   MAGDGFGFPVESQAVSVKKKTATTTTTTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAL 60

Query: 61  MQRVQIHARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPI 120
           MQRVQI+ARDLRLLDPLLSYPSTILGRE VIVLNLEHIKSIITADEVLLRDPMDENVVPI
Sbjct: 61  MQRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPI 120

Query: 121 VEELQRRLPSTSSIYQGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTRE 180
           VEELQRRLPST+S+YQGQGEEEEP TTQNELAENEFPFEFRALEVALEAICSFLDARTRE
Sbjct: 121 VEELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTRE 180

Query: 181 LETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVVRDELEQLLDDDDDMAELY 240
           LETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQK VRDELEQLLDDDDDMAELY
Sbjct: 181 LETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQK-VRDELEQLLDDDDDMAELY 240

Query: 241 LSRKVAGMYTPESGSGTPNWLLASPKDYYSKMSKTSRASLATIRGENDVEELEMLLEASS 300
           LSRKVAG  TPESGSGTP W LASPKD YSKMSKTSRAS  T RGENDVEELEMLLE   
Sbjct: 241 LSRKVAG--TPESGSGTPIWFLASPKD-YSKMSKTSRASAITFRGENDVEELEMLLE--- 300

Query: 301 FILNVLQAYFMQIESTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLS 360
                  AYFMQIE TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLS
Sbjct: 301 -------AYFMQIEGTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLS 360

Query: 361 IYSLVTAIFGMNIPYTWKEDHEHVFKWVVIVTGIVCASIYLTLSSYARYKGLVES 405
           IYSLVTAIFGMNIPYTWKEDHEHVFKWVVI+TGIVCA+IY++LSSYAR KGLV S
Sbjct: 361 IYSLVTAIFGMNIPYTWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 401

BLAST of MS020311 vs. NCBI nr
Match: KAA0057420.1 (magnesium transporter MRS2-I-like isoform X2 [Cucumis melo var. makuwa] >TYK30119.1 magnesium transporter MRS2-I-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 669.1 bits (1725), Expect = 2.4e-188
Identity = 362/409 (88.51%), Postives = 376/409 (91.93%), Query Frame = 0

Query: 1   MAGDAFLIPVESQ-ASVKKK-----TKKK-TVISRSWVSLDHEGRSTVLDVDKYAIMQRV 60
           M GD F+IPVESQ +SVKKK     TKKK T +SRSWVSLD +GRST+LDVDKYAIM+R 
Sbjct: 1   MGGDGFVIPVESQVSSVKKKKKQSTTKKKTTAVSRSWVSLDRDGRSTILDVDKYAIMERA 60

Query: 61  QIHARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEEL 120
           QI+ARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDP DENVVPIVEEL
Sbjct: 61  QINARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPTDENVVPIVEEL 120

Query: 121 QRRLPSTSSIYQGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETD 180
           QRRLPST+S+YQGQGEEEEP TTQNELAENEFPFEFRALEVALEAICSFLDARTRELETD
Sbjct: 121 QRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETD 180

Query: 181 TYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVVRDELEQLLDDDDDMAELYLSRK 240
           TYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVVRDELEQLLDDDDDMAELYLSRK
Sbjct: 181 TYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVVRDELEQLLDDDDDMAELYLSRK 240

Query: 241 VAGMYTPESGSGTPNWLLASPKDYYSKMSKTSRASLATIRGENDVEELEMLLEASSFILN 300
           VAG  TPESGSGTP W LASPKD YSK+S+TSR S  TIRGENDVEELEMLLE       
Sbjct: 241 VAG--TPESGSGTPIWFLASPKD-YSKISRTSRVSAITIRGENDVEELEMLLE------- 300

Query: 301 VLQAYFMQIESTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSL 360
              AYFMQIE TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSL
Sbjct: 301 ---AYFMQIEGTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSL 360

Query: 361 VTAIFGMNIPYTWKEDHEHVFKWVVIVTGIVCASIYLTLSSYARYKGLV 403
           VTAIFGMNIPYTWKEDHEHVFKWVVIVTGIVCA+IY++LSSYARYKGLV
Sbjct: 361 VTAIFGMNIPYTWKEDHEHVFKWVVIVTGIVCATIYVSLSSYARYKGLV 396

BLAST of MS020311 vs. NCBI nr
Match: XP_022963506.1 (magnesium transporter MRS2-I-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 668.7 bits (1724), Expect = 3.2e-188
Identity = 367/414 (88.65%), Postives = 375/414 (90.58%), Query Frame = 0

Query: 1   MAGDAFLIPVESQA-SVKKKT---------KKKTVISRSWVSLDHEGRSTVLDVDKYAIM 60
           MAGD F  PVESQA SVKKKT         KK TV+SRSWVSLDHEGRSTVLDVDKYAIM
Sbjct: 1   MAGDGFGFPVESQAVSVKKKTAATTTTTTKKKNTVVSRSWVSLDHEGRSTVLDVDKYAIM 60

Query: 61  QRVQIHARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120
           QRVQI+ARDLRLLDPLLSYPSTILGRE VIVLNLEHIKSIITADEVLLRDPMDENVVPIV
Sbjct: 61  QRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120

Query: 121 EELQRRLPSTSSIYQGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTREL 180
           EELQRRLPST+S+YQGQGEEEEP  TQNELAENEFPFEFRALEVALEAICSFLDARTREL
Sbjct: 121 EELQRRLPSTNSLYQGQGEEEEPSHTQNELAENEFPFEFRALEVALEAICSFLDARTREL 180

Query: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVVRDELEQLLDDDDDMAELYL 240
           ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQK VRDELEQLLDDDDDMAELYL
Sbjct: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQK-VRDELEQLLDDDDDMAELYL 240

Query: 241 SRKVAGMYTPESGSGTPNWLLASPKDYYSKMSKTSRASLATIRGENDVEELEMLLEASSF 300
           SRKVAG  TPESGSGTP W LASPKD YSKMSKTSRAS  T RGENDVEELEMLLE    
Sbjct: 241 SRKVAG--TPESGSGTPIWFLASPKD-YSKMSKTSRASAITFRGENDVEELEMLLE---- 300

Query: 301 ILNVLQAYFMQIESTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSI 360
                 AYFMQIE TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSI
Sbjct: 301 ------AYFMQIEGTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSI 360

Query: 361 YSLVTAIFGMNIPYTWKEDHEHVFKWVVIVTGIVCASIYLTLSSYARYKGLVES 405
           YSLVTAIFGMNIPYTWKEDHEHVFKWVVI+TGIVCA+IY++LSSYAR KGLV S
Sbjct: 361 YSLVTAIFGMNIPYTWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 400

BLAST of MS020311 vs. ExPASy Swiss-Prot
Match: Q9FLG2 (Magnesium transporter MRS2-2 OS=Arabidopsis thaliana OX=3702 GN=MRS2-2 PE=2 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 2.2e-139
Identity = 281/409 (68.70%), Postives = 319/409 (78.00%), Query Frame = 0

Query: 1   MAGDAFLIPVESQASVKKKTKKKTVISRSWVSLDHEGRSTVLDVDKYAIMQRVQIHARDL 60
           MA + +L+P +  A V   T KK     SW  +D  G+S  LDVDKY IM RVQIHARDL
Sbjct: 1   MAQNGYLVPADPSAVV---TVKKKTPQASWALIDATGQSEPLDVDKYEIMHRVQIHARDL 60

Query: 61  RLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEELQRRLP-S 120
           R+LDP LSYPSTILGRER IVLNLEHIK+IIT++EVLLRDP DENV+P+VEEL+RRLP  
Sbjct: 61  RILDPNLSYPSTILGRERAIVLNLEHIKAIITSEEVLLRDPSDENVIPVVEELRRRLPVG 120

Query: 121 TSSIYQGQGEEEEPPTTQNE---LAENEFPFEFRALEVALEAICSFLDARTRELETDTYP 180
            +S   GQG+ +E    QN+     E+E PFEFRALEVALEAICSFL ART ELET  YP
Sbjct: 121 NASHNGGQGDGKEIAGAQNDGDTGDEDESPFEFRALEVALEAICSFLAARTAELETAAYP 180

Query: 181 ALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVVRDELEQLLDDDDDMAELYLSRKVAG 240
           ALDELTSKISSRNLDRVRKLKSAMTRLT RVQK VRDELEQLLDDDDDMA+LYLSRK++ 
Sbjct: 181 ALDELTSKISSRNLDRVRKLKSAMTRLTARVQK-VRDELEQLLDDDDDMADLYLSRKLSS 240

Query: 241 MYTPESGSGTPNWLLASPKDYYSKMSKTSRASLATIRG-ENDVEELEMLLEASSFILNVL 300
             +P S  G PNW   SP    SK+S+ SRASLAT+ G ENDVEELEMLLE         
Sbjct: 241 ASSPISSIGEPNWYTTSP-TIGSKISRASRASLATVHGDENDVEELEMLLE--------- 300

Query: 301 QAYFMQIESTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVT 360
            AYFMQI+STLN+L TLREYIDDTEDYINIQLDNHRNQLIQLEL LSSGTVCLS+YSLV 
Sbjct: 301 -AYFMQIDSTLNRLTTLREYIDDTEDYINIQLDNHRNQLIQLELVLSSGTVCLSMYSLVA 360

Query: 361 AIFGMNIPYTWKEDHEHVFKWVVIVTGIVCASIYLTLSSYARYKGLVES 405
            IFGMNIPYTW + H ++FK+VV +TG +C  +++ + SYARYKGLV S
Sbjct: 361 GIFGMNIPYTWNDGHGYMFKYVVGLTGTLCVVVFVIIMSYARYKGLVGS 394

BLAST of MS020311 vs. ExPASy Swiss-Prot
Match: B8AJT9 (Magnesium transporter MRS2-I OS=Oryza sativa subsp. indica OX=39946 GN=MRS2-I PE=3 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 3.7e-139
Identity = 273/385 (70.91%), Postives = 310/385 (80.52%), Query Frame = 0

Query: 22  KKTVISRSWVSLDHEGRSTVLDVDKYAIMQRVQIHARDLRLLDPLLSYPSTILGRERVIV 81
           KK   SRSW+  D  G   VLD DKYAIM RV I+ARDLR+LDPLLSYPSTILGRER IV
Sbjct: 20  KKRGASRSWILFDAAGEERVLDADKYAIMHRVDINARDLRILDPLLSYPSTILGRERAIV 79

Query: 82  LNLEHIKSIITADEVLLRDPMDENVVPIVEELQRRL-PSTSSIYQGQGEEEEPPTTQNEL 141
           LNLEHIK+IITA+EVLLRDP+D+NV+P+VEEL+RRL PS+++ +  +G EE+        
Sbjct: 80  LNLEHIKAIITAEEVLLRDPLDDNVIPVVEELRRRLAPSSATQHDVEGAEED-------- 139

Query: 142 AENEFPFEFRALEVALEAICSFLDARTRELETDTYPALDELTSKISSRNLDRVRKLKSAM 201
              E PFEFRALEV LEAICSFL ART ELE+  YPALDELTSKISSRNLDRVRKLKS M
Sbjct: 140 ---ESPFEFRALEVTLEAICSFLGARTTELESAAYPALDELTSKISSRNLDRVRKLKSGM 199

Query: 202 TRLTNRVQKVVRDELEQLLDDDDDMAELYLSRKVAGMYTPESGSGTPNWLLASPKDYYSK 261
           TRL  RVQK VRDELEQLLDDDDDMA+LYLSRK+AG  +P SGSG PNW  ASP    SK
Sbjct: 200 TRLNARVQK-VRDELEQLLDDDDDMADLYLSRKLAGAASPVSGSGGPNWFPASP-TIGSK 259

Query: 262 MSKTSRASLATIRG-ENDVEELEMLLEASSFILNVLQAYFMQIESTLNKLITLREYIDDT 321
           +S+ SRAS  TI G ENDVEELEMLLE          AYFMQI+ TLNKL TLREYIDDT
Sbjct: 260 ISRASRASAPTIHGNENDVEELEMLLE----------AYFMQIDGTLNKLTTLREYIDDT 319

Query: 322 EDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPYTWKEDHEHVFKWVVI 381
           EDYINIQLDNHRNQLIQLELFLSSGTVCLS+YSLV  IFGMNIPYTW ++H +VFKWVV+
Sbjct: 320 EDYINIQLDNHRNQLIQLELFLSSGTVCLSLYSLVAGIFGMNIPYTWNDNHGYVFKWVVL 379

Query: 382 VTGIVCASIYLTLSSYARYKGLVES 405
           V+G+ CA +++++ +YAR+KGLV S
Sbjct: 380 VSGLFCAFMFVSIVAYARHKGLVGS 381

BLAST of MS020311 vs. ExPASy Swiss-Prot
Match: Q10D38 (Magnesium transporter MRS2-I OS=Oryza sativa subsp. japonica OX=39947 GN=MRS2-I PE=2 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 3.7e-139
Identity = 273/385 (70.91%), Postives = 310/385 (80.52%), Query Frame = 0

Query: 22  KKTVISRSWVSLDHEGRSTVLDVDKYAIMQRVQIHARDLRLLDPLLSYPSTILGRERVIV 81
           KK   SRSW+  D  G   VLD DKYAIM RV I+ARDLR+LDPLLSYPSTILGRER IV
Sbjct: 23  KKRGASRSWILFDAAGEERVLDADKYAIMHRVDINARDLRILDPLLSYPSTILGRERAIV 82

Query: 82  LNLEHIKSIITADEVLLRDPMDENVVPIVEELQRRL-PSTSSIYQGQGEEEEPPTTQNEL 141
           LNLEHIK+IITA+EVLLRDP+D+NV+P+VEEL+RRL PS+++ +  +G EE+        
Sbjct: 83  LNLEHIKAIITAEEVLLRDPLDDNVIPVVEELRRRLAPSSATQHDVEGAEED-------- 142

Query: 142 AENEFPFEFRALEVALEAICSFLDARTRELETDTYPALDELTSKISSRNLDRVRKLKSAM 201
              E PFEFRALEV LEAICSFL ART ELE+  YPALDELTSKISSRNLDRVRKLKS M
Sbjct: 143 ---ESPFEFRALEVTLEAICSFLGARTTELESAAYPALDELTSKISSRNLDRVRKLKSGM 202

Query: 202 TRLTNRVQKVVRDELEQLLDDDDDMAELYLSRKVAGMYTPESGSGTPNWLLASPKDYYSK 261
           TRL  RVQK VRDELEQLLDDDDDMA+LYLSRK+AG  +P SGSG PNW  ASP    SK
Sbjct: 203 TRLNARVQK-VRDELEQLLDDDDDMADLYLSRKLAGAASPVSGSGGPNWFPASP-TIGSK 262

Query: 262 MSKTSRASLATIRG-ENDVEELEMLLEASSFILNVLQAYFMQIESTLNKLITLREYIDDT 321
           +S+ SRAS  TI G ENDVEELEMLLE          AYFMQI+ TLNKL TLREYIDDT
Sbjct: 263 ISRASRASAPTIHGNENDVEELEMLLE----------AYFMQIDGTLNKLTTLREYIDDT 322

Query: 322 EDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPYTWKEDHEHVFKWVVI 381
           EDYINIQLDNHRNQLIQLELFLSSGTVCLS+YSLV  IFGMNIPYTW ++H +VFKWVV+
Sbjct: 323 EDYINIQLDNHRNQLIQLELFLSSGTVCLSLYSLVAGIFGMNIPYTWNDNHGYVFKWVVL 382

Query: 382 VTGIVCASIYLTLSSYARYKGLVES 405
           V+G+ CA +++++ +YAR+KGLV S
Sbjct: 383 VSGLFCAFMFVSIVAYARHKGLVGS 384

BLAST of MS020311 vs. ExPASy Swiss-Prot
Match: Q304A0 (Magnesium transporter MRS2-7 OS=Arabidopsis thaliana OX=3702 GN=MRS2-7 PE=2 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 4.1e-130
Identity = 262/408 (64.22%), Postives = 307/408 (75.25%), Query Frame = 0

Query: 1   MAGDAFLIPVESQASVKKKTKKKTVISRSWVSLDHEGRSTVLDVDKYAIMQRVQIHARDL 60
           M+ D  L+PV+S A V  K +K + +SRSW+S+D  G+ TVLDVDK+ IM RVQIHARDL
Sbjct: 1   MSPDGELVPVDSSAVVTAK-RKTSQLSRSWISIDATGQKTVLDVDKHVIMHRVQIHARDL 60

Query: 61  RLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEELQRRLPST 120
           R+LDP L YPS ILGRER IVLNLEHIK+IITA+EVL+RD  DENV+P++EE QRRLP  
Sbjct: 61  RILDPNLFYPSAILGRERAIVLNLEHIKAIITAEEVLIRDSSDENVIPVLEEFQRRLPVG 120

Query: 121 SSIY--QGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETDTYPAL 180
           +  +   G G+  E         E+E PFEFRALEVALEAICSFL ART ELE   YPAL
Sbjct: 121 NEAHGVHGDGDLGE---------EDESPFEFRALEVALEAICSFLAARTTELEKFAYPAL 180

Query: 181 DELTSKISSRNLDRVRKLKSAMTRLTNRVQKVVRDELEQLLDDDDDMAELYLSRKVAGMY 240
           DELT KISSRNL+RVRKLKSAMTRLT RVQK VRDELEQLLDDD DMA+LYL+RK+ G  
Sbjct: 181 DELTLKISSRNLERVRKLKSAMTRLTARVQK-VRDELEQLLDDDGDMADLYLTRKLVGAS 240

Query: 241 TPESGSGTPNWLLASPKDYYSKMSKTSRASLATIRG--ENDVEELEMLLEASSFILNVLQ 300
           +  S S  P W   SP    S +S+ SR SL T+RG  E DVEELEMLLE          
Sbjct: 241 SSVSVSDEPIWYPTSP-TIGSMISRASRVSLVTVRGDDETDVEELEMLLE---------- 300

Query: 301 AYFMQIESTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTA 360
           AYFMQI+STLNKL  LREYIDDTEDYINIQLDNHRNQLIQLEL LS+GTVC+S+YS++  
Sbjct: 301 AYFMQIDSTLNKLTELREYIDDTEDYINIQLDNHRNQLIQLELMLSAGTVCVSVYSMIAG 360

Query: 361 IFGMNIPYTWKEDHEHVFKWVVIVTGIVCASIYLTLSSYARYKGLVES 405
           IFGMNIP TW  DH ++FKWVV +TG  C  +++ + SYAR++GL+ S
Sbjct: 361 IFGMNIPNTWNHDHGYIFKWVVSLTGTFCIVLFVIILSYARFRGLIGS 386

BLAST of MS020311 vs. ExPASy Swiss-Prot
Match: P0CZ21 (Magnesium transporter MRS2-8 OS=Arabidopsis thaliana OX=3702 GN=MRS2-8 PE=2 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 4.4e-108
Identity = 229/400 (57.25%), Postives = 289/400 (72.25%), Query Frame = 0

Query: 7   LIPVESQASVKKKTKKKTVISRSWVSLDHEGRSTVLDVDKYAIMQRVQIHARDLRLLDPL 66
           ++P E    VK+ T +    S SW+S+D  G+ TVLDVDKY IM RVQIHARDLR+LDP 
Sbjct: 1   MLPNEELVPVKRITPQS---SWSWISIDATGKKTVLDVDKYVIMHRVQIHARDLRILDPN 60

Query: 67  LSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEELQRRLPSTSSIYQG 126
           L YPS ILGRER IVLNLEHIK+IITA EVL++D  DEN++P +EE Q RL   +  + G
Sbjct: 61  LFYPSAILGRERAIVLNLEHIKAIITAKEVLIQDSSDENLIPTLEEFQTRLSVGNKAHGG 120

Query: 127 QGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETDTYPALDELTSKIS 186
           Q + +        + E+E  FEFRALEVALEAICSFL ART ELE   YPALDELT K++
Sbjct: 121 QLDGD-------VVEEDESAFEFRALEVALEAICSFLAARTIELEKSAYPALDELTLKLT 180

Query: 187 SRNLDRVRKLKSAMTRLTNRVQKVVRDELEQLLDDDDDMAELYLSRKVAGMYTPESGSGT 246
           SRNL RV KLKS+MTRLT +VQK ++DELEQLL+DD+DMAELYLSRK+AG  +P   SG 
Sbjct: 181 SRNLLRVCKLKSSMTRLTAQVQK-IKDELEQLLEDDEDMAELYLSRKLAGASSPAIDSGE 240

Query: 247 P-NWLLASPKDYYSKMS--KTSRASLATIRGE--NDVEELEMLLEASSFILNVLQAYFMQ 306
             NW   SP    +K+S  K+     AT+RG+  NDVEE+EMLLE          A+FMQ
Sbjct: 241 HINWYPTSP-TIGAKISRAKSHLVRSATVRGDDKNDVEEVEMLLE----------AHFMQ 300

Query: 307 IESTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMN 366
           I+ TLNKL  LREY+D+TED++NIQLD+ RNQLI+ E+ L++G++C+S+YS+V  I GMN
Sbjct: 301 IDRTLNKLTELREYVDETEDFLNIQLDSSRNQLIKFEIILTAGSICVSVYSVVVGILGMN 360

Query: 367 IPYTWKEDHEHVFKWVVIVTGIVCASIYLTLSSYARYKGL 402
           IP+ W    +H+FKWVV  T  VCA +++T+ S+ARYK L
Sbjct: 361 IPFPW-NIKKHMFKWVVSGTATVCAILFVTIMSFARYKKL 377

BLAST of MS020311 vs. ExPASy TrEMBL
Match: A0A6J1CYA8 (Magnesium transporter OS=Momordica charantia OX=3673 GN=LOC111015319 PE=3 SV=1)

HSP 1 Score: 742.7 bits (1916), Expect = 8.4e-211
Identity = 393/404 (97.28%), Postives = 393/404 (97.28%), Query Frame = 0

Query: 1   MAGDAFLIPVESQASVKKKTKKKTVISRSWVSLDHEGRSTVLDVDKYAIMQRVQIHARDL 60
           MAGDAFLIPVESQASVKKKTKKKTVISRSWVSLDHEGRSTVLDVDKYAIMQRVQIHARDL
Sbjct: 1   MAGDAFLIPVESQASVKKKTKKKTVISRSWVSLDHEGRSTVLDVDKYAIMQRVQIHARDL 60

Query: 61  RLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEELQRRLPST 120
           RLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEELQRRLPST
Sbjct: 61  RLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEELQRRLPST 120

Query: 121 SSIYQGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETDTYPALDE 180
           SSIYQGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETDTYPALDE
Sbjct: 121 SSIYQGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETDTYPALDE 180

Query: 181 LTSKISSRNLDRVRKLKSAMTRLTNRVQKVVRDELEQLLDDDDDMAELYLSRKVAGMYTP 240
           LTSKISSRNLDRVRKLKSAMTRLTNRVQK VRDELEQLLDDDDDMAELYLSRKVAGMYTP
Sbjct: 181 LTSKISSRNLDRVRKLKSAMTRLTNRVQK-VRDELEQLLDDDDDMAELYLSRKVAGMYTP 240

Query: 241 ESGSGTPNWLLASPKDYYSKMSKTSRASLATIRGENDVEELEMLLEASSFILNVLQAYFM 300
           ESGSGTPNWLLASPKDYYSKMSKTSRASLATIRGENDVEELEMLLE          AYFM
Sbjct: 241 ESGSGTPNWLLASPKDYYSKMSKTSRASLATIRGENDVEELEMLLE----------AYFM 300

Query: 301 QIESTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGM 360
           QIESTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGM
Sbjct: 301 QIESTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGM 360

Query: 361 NIPYTWKEDHEHVFKWVVIVTGIVCASIYLTLSSYARYKGLVES 405
           NIPYTWKEDHEHVFKWVVIVTGIVCASIYLTLSSYARYKGLVES
Sbjct: 361 NIPYTWKEDHEHVFKWVVIVTGIVCASIYLTLSSYARYKGLVES 393

BLAST of MS020311 vs. ExPASy TrEMBL
Match: A0A5D3E2W6 (Magnesium transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G001010 PE=3 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 1.2e-188
Identity = 362/409 (88.51%), Postives = 376/409 (91.93%), Query Frame = 0

Query: 1   MAGDAFLIPVESQ-ASVKKK-----TKKK-TVISRSWVSLDHEGRSTVLDVDKYAIMQRV 60
           M GD F+IPVESQ +SVKKK     TKKK T +SRSWVSLD +GRST+LDVDKYAIM+R 
Sbjct: 1   MGGDGFVIPVESQVSSVKKKKKQSTTKKKTTAVSRSWVSLDRDGRSTILDVDKYAIMERA 60

Query: 61  QIHARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEEL 120
           QI+ARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDP DENVVPIVEEL
Sbjct: 61  QINARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPTDENVVPIVEEL 120

Query: 121 QRRLPSTSSIYQGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETD 180
           QRRLPST+S+YQGQGEEEEP TTQNELAENEFPFEFRALEVALEAICSFLDARTRELETD
Sbjct: 121 QRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETD 180

Query: 181 TYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVVRDELEQLLDDDDDMAELYLSRK 240
           TYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVVRDELEQLLDDDDDMAELYLSRK
Sbjct: 181 TYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVVRDELEQLLDDDDDMAELYLSRK 240

Query: 241 VAGMYTPESGSGTPNWLLASPKDYYSKMSKTSRASLATIRGENDVEELEMLLEASSFILN 300
           VAG  TPESGSGTP W LASPKD YSK+S+TSR S  TIRGENDVEELEMLLE       
Sbjct: 241 VAG--TPESGSGTPIWFLASPKD-YSKISRTSRVSAITIRGENDVEELEMLLE------- 300

Query: 301 VLQAYFMQIESTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSL 360
              AYFMQIE TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSL
Sbjct: 301 ---AYFMQIEGTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSL 360

Query: 361 VTAIFGMNIPYTWKEDHEHVFKWVVIVTGIVCASIYLTLSSYARYKGLV 403
           VTAIFGMNIPYTWKEDHEHVFKWVVIVTGIVCA+IY++LSSYARYKGLV
Sbjct: 361 VTAIFGMNIPYTWKEDHEHVFKWVVIVTGIVCATIYVSLSSYARYKGLV 396

BLAST of MS020311 vs. ExPASy TrEMBL
Match: A0A6J1HGA4 (Magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111463817 PE=3 SV=1)

HSP 1 Score: 668.7 bits (1724), Expect = 1.5e-188
Identity = 367/414 (88.65%), Postives = 375/414 (90.58%), Query Frame = 0

Query: 1   MAGDAFLIPVESQA-SVKKKT---------KKKTVISRSWVSLDHEGRSTVLDVDKYAIM 60
           MAGD F  PVESQA SVKKKT         KK TV+SRSWVSLDHEGRSTVLDVDKYAIM
Sbjct: 1   MAGDGFGFPVESQAVSVKKKTAATTTTTTKKKNTVVSRSWVSLDHEGRSTVLDVDKYAIM 60

Query: 61  QRVQIHARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120
           QRVQI+ARDLRLLDPLLSYPSTILGRE VIVLNLEHIKSIITADEVLLRDPMDENVVPIV
Sbjct: 61  QRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120

Query: 121 EELQRRLPSTSSIYQGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTREL 180
           EELQRRLPST+S+YQGQGEEEEP  TQNELAENEFPFEFRALEVALEAICSFLDARTREL
Sbjct: 121 EELQRRLPSTNSLYQGQGEEEEPSHTQNELAENEFPFEFRALEVALEAICSFLDARTREL 180

Query: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVVRDELEQLLDDDDDMAELYL 240
           ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQK VRDELEQLLDDDDDMAELYL
Sbjct: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQK-VRDELEQLLDDDDDMAELYL 240

Query: 241 SRKVAGMYTPESGSGTPNWLLASPKDYYSKMSKTSRASLATIRGENDVEELEMLLEASSF 300
           SRKVAG  TPESGSGTP W LASPKD YSKMSKTSRAS  T RGENDVEELEMLLE    
Sbjct: 241 SRKVAG--TPESGSGTPIWFLASPKD-YSKMSKTSRASAITFRGENDVEELEMLLE---- 300

Query: 301 ILNVLQAYFMQIESTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSI 360
                 AYFMQIE TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSI
Sbjct: 301 ------AYFMQIEGTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSI 360

Query: 361 YSLVTAIFGMNIPYTWKEDHEHVFKWVVIVTGIVCASIYLTLSSYARYKGLVES 405
           YSLVTAIFGMNIPYTWKEDHEHVFKWVVI+TGIVCA+IY++LSSYAR KGLV S
Sbjct: 361 YSLVTAIFGMNIPYTWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 400

BLAST of MS020311 vs. ExPASy TrEMBL
Match: A0A6J1HR33 (Magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111467011 PE=3 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 2.6e-188
Identity = 365/411 (88.81%), Postives = 374/411 (91.00%), Query Frame = 0

Query: 1   MAGDAFLIPVESQ-ASVKKKT------KKKTVISRSWVSLDHEGRSTVLDVDKYAIMQRV 60
           MAGD F  PVESQ  SVKKKT      KK TV+SRSWVSLDHEGR+TVLDVDKYAIMQRV
Sbjct: 1   MAGDGFGFPVESQVVSVKKKTATTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAIMQRV 60

Query: 61  QIHARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEEL 120
           QI+ARDLRLLDPLLSYPSTILGRE VIVLNLEHIKSIITADEVLLRDPMDENVVPIVEEL
Sbjct: 61  QINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEEL 120

Query: 121 QRRLPSTSSIYQGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETD 180
           QRRLPST+S+YQGQGEEEEP TTQNELAENEFPFEFRALEVALEAICSFLDARTRELETD
Sbjct: 121 QRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETD 180

Query: 181 TYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVVRDELEQLLDDDDDMAELYLSRK 240
            YPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQK VRDELEQLLDDDDDMAELYLSRK
Sbjct: 181 AYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQK-VRDELEQLLDDDDDMAELYLSRK 240

Query: 241 VAGMYTPESGSGTPNWLLASPKDYYSKMSKTSRASLATIRGENDVEELEMLLEASSFILN 300
           VAG  TPESGSGTP W LASPKD YSKMSKTSRAS  T RGENDVEELEMLLE       
Sbjct: 241 VAG--TPESGSGTPIWFLASPKD-YSKMSKTSRASAITFRGENDVEELEMLLE------- 300

Query: 301 VLQAYFMQIESTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSL 360
              AYFMQIE TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSL
Sbjct: 301 ---AYFMQIEGTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSL 360

Query: 361 VTAIFGMNIPYTWKEDHEHVFKWVVIVTGIVCASIYLTLSSYARYKGLVES 405
           VTAIFGMNIPYTWKEDHEHVFKWVVI+TGIVCA+IY++LSSYAR KGLV S
Sbjct: 361 VTAIFGMNIPYTWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 397

BLAST of MS020311 vs. ExPASy TrEMBL
Match: A0A0A0LLQ5 (Magnesium transporter OS=Cucumis sativus OX=3659 GN=Csa_2G225310 PE=3 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 3.4e-188
Identity = 364/411 (88.56%), Postives = 377/411 (91.73%), Query Frame = 0

Query: 1   MAGDAFLIPVESQ-ASVKKK-----TKKK-TVISRSWVSLDHEGRSTVLDVDKYAIMQRV 60
           M GD F+IPVESQ +SVKKK     TKKK T +SRSWVSLD EGRST+LDVDKY IM+RV
Sbjct: 1   MGGDGFVIPVESQVSSVKKKKKQTTTKKKTTAVSRSWVSLDREGRSTILDVDKYVIMERV 60

Query: 61  QIHARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEEL 120
           QI+ARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEEL
Sbjct: 61  QINARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEEL 120

Query: 121 QRRLPSTSSIYQGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETD 180
           QRRLPST+S+YQGQGEEEEP TTQNELAENEFPFEFRALEVALEAICSFLDARTRELETD
Sbjct: 121 QRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETD 180

Query: 181 TYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVVRDELEQLLDDDDDMAELYLSRK 240
           TYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQK VRDELEQLLDDDDDMAELYLSRK
Sbjct: 181 TYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQK-VRDELEQLLDDDDDMAELYLSRK 240

Query: 241 VAGMYTPESGSGTPNWLLASPKDYYSKMSKTSRASLATIRGENDVEELEMLLEASSFILN 300
           VAG  TPESGSGTP W LASPKD YSK+S+TSR S  TIRGENDVEELEMLLE       
Sbjct: 241 VAG--TPESGSGTPIWFLASPKD-YSKISRTSRVSAITIRGENDVEELEMLLE------- 300

Query: 301 VLQAYFMQIESTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSL 360
              AYFMQIE TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSL
Sbjct: 301 ---AYFMQIEGTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSL 360

Query: 361 VTAIFGMNIPYTWKEDHEHVFKWVVIVTGIVCASIYLTLSSYARYKGLVES 405
           VTAIFGMNIPYTWKEDHEHVFKWVVIVTGIVCA+IY++LSSYARYKGLV S
Sbjct: 361 VTAIFGMNIPYTWKEDHEHVFKWVVIVTGIVCATIYVSLSSYARYKGLVGS 397

BLAST of MS020311 vs. TAIR 10
Match: AT5G64560.1 (magnesium transporter 9 )

HSP 1 Score: 496.9 bits (1278), Expect = 1.5e-140
Identity = 281/409 (68.70%), Postives = 319/409 (78.00%), Query Frame = 0

Query: 1   MAGDAFLIPVESQASVKKKTKKKTVISRSWVSLDHEGRSTVLDVDKYAIMQRVQIHARDL 60
           MA + +L+P +  A V   T KK     SW  +D  G+S  LDVDKY IM RVQIHARDL
Sbjct: 1   MAQNGYLVPADPSAVV---TVKKKTPQASWALIDATGQSEPLDVDKYEIMHRVQIHARDL 60

Query: 61  RLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEELQRRLP-S 120
           R+LDP LSYPSTILGRER IVLNLEHIK+IIT++EVLLRDP DENV+P+VEEL+RRLP  
Sbjct: 61  RILDPNLSYPSTILGRERAIVLNLEHIKAIITSEEVLLRDPSDENVIPVVEELRRRLPVG 120

Query: 121 TSSIYQGQGEEEEPPTTQNE---LAENEFPFEFRALEVALEAICSFLDARTRELETDTYP 180
            +S   GQG+ +E    QN+     E+E PFEFRALEVALEAICSFL ART ELET  YP
Sbjct: 121 NASHNGGQGDGKEIAGAQNDGDTGDEDESPFEFRALEVALEAICSFLAARTAELETAAYP 180

Query: 181 ALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVVRDELEQLLDDDDDMAELYLSRKVAG 240
           ALDELTSKISSRNLDRVRKLKSAMTRLT RVQK VRDELEQLLDDDDDMA+LYLSRK++ 
Sbjct: 181 ALDELTSKISSRNLDRVRKLKSAMTRLTARVQK-VRDELEQLLDDDDDMADLYLSRKLSS 240

Query: 241 MYTPESGSGTPNWLLASPKDYYSKMSKTSRASLATIRG-ENDVEELEMLLEASSFILNVL 300
             +P S  G PNW   SP    SK+S+ SRASLAT+ G ENDVEELEMLLE         
Sbjct: 241 ASSPISSIGEPNWYTTSP-TIGSKISRASRASLATVHGDENDVEELEMLLE--------- 300

Query: 301 QAYFMQIESTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVT 360
            AYFMQI+STLN+L TLREYIDDTEDYINIQLDNHRNQLIQLEL LSSGTVCLS+YSLV 
Sbjct: 301 -AYFMQIDSTLNRLTTLREYIDDTEDYINIQLDNHRNQLIQLELVLSSGTVCLSMYSLVA 360

Query: 361 AIFGMNIPYTWKEDHEHVFKWVVIVTGIVCASIYLTLSSYARYKGLVES 405
            IFGMNIPYTW + H ++FK+VV +TG +C  +++ + SYARYKGLV S
Sbjct: 361 GIFGMNIPYTWNDGHGYMFKYVVGLTGTLCVVVFVIIMSYARYKGLVGS 394

BLAST of MS020311 vs. TAIR 10
Match: AT5G64560.2 (magnesium transporter 9 )

HSP 1 Score: 468.8 bits (1205), Expect = 4.5e-132
Identity = 267/382 (69.90%), Postives = 299/382 (78.27%), Query Frame = 0

Query: 1   MAGDAFLIPVESQASVKKKTKKKTVISRSWVSLDHEGRSTVLDVDKYAIMQRVQIHARDL 60
           MA + +L+P +  A V   T KK     SW  +D  G+S  LDVDKY IM RVQIHARDL
Sbjct: 1   MAQNGYLVPADPSAVV---TVKKKTPQASWALIDATGQSEPLDVDKYEIMHRVQIHARDL 60

Query: 61  RLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEELQRRLP-S 120
           R+LDP LSYPSTILGRER IVLNLEHIK+IIT++EVLLRDP DENV+P+VEEL+RRLP  
Sbjct: 61  RILDPNLSYPSTILGRERAIVLNLEHIKAIITSEEVLLRDPSDENVIPVVEELRRRLPVG 120

Query: 121 TSSIYQGQGEEEEPPTTQNE---LAENEFPFEFRALEVALEAICSFLDARTRELETDTYP 180
            +S   GQG+ +E    QN+     E+E PFEFRALEVALEAICSFL ART ELET  YP
Sbjct: 121 NASHNGGQGDGKEIAGAQNDGDTGDEDESPFEFRALEVALEAICSFLAARTAELETAAYP 180

Query: 181 ALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVVRDELEQLLDDDDDMAELYLSRKVAG 240
           ALDELTSKISSRNLDRVRKLKSAMTRLT RVQK VRDELEQLLDDDDDMA+LYLSRK++ 
Sbjct: 181 ALDELTSKISSRNLDRVRKLKSAMTRLTARVQK-VRDELEQLLDDDDDMADLYLSRKLSS 240

Query: 241 MYTPESGSGTPNWLLASPKDYYSKMSKTSRASLATIRG-ENDVEELEMLLEASSFILNVL 300
             +P S  G PNW   SP    SK+S+ SRASLAT+ G ENDVEELEMLLE         
Sbjct: 241 ASSPISSIGEPNWYTTSP-TIGSKISRASRASLATVHGDENDVEELEMLLE--------- 300

Query: 301 QAYFMQIESTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVT 360
            AYFMQI+STLN+L TLREYIDDTEDYINIQLDNHRNQLIQLEL LSSGTVCLS+YSLV 
Sbjct: 301 -AYFMQIDSTLNRLTTLREYIDDTEDYINIQLDNHRNQLIQLELVLSSGTVCLSMYSLVA 360

Query: 361 AIFGMNIPYTWKEDHEHVFKWV 378
            IFGMNIPYTW + H ++FK+V
Sbjct: 361 GIFGMNIPYTWNDGHGYMFKYV 367

BLAST of MS020311 vs. TAIR 10
Match: AT5G09690.1 (magnesium transporter 7 )

HSP 1 Score: 466.1 bits (1198), Expect = 2.9e-131
Identity = 262/408 (64.22%), Postives = 307/408 (75.25%), Query Frame = 0

Query: 1   MAGDAFLIPVESQASVKKKTKKKTVISRSWVSLDHEGRSTVLDVDKYAIMQRVQIHARDL 60
           M+ D  L+PV+S A V  K +K + +SRSW+S+D  G+ TVLDVDK+ IM RVQIHARDL
Sbjct: 1   MSPDGELVPVDSSAVVTAK-RKTSQLSRSWISIDATGQKTVLDVDKHVIMHRVQIHARDL 60

Query: 61  RLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEELQRRLPST 120
           R+LDP L YPS ILGRER IVLNLEHIK+IITA+EVL+RD  DENV+P++EE QRRLP  
Sbjct: 61  RILDPNLFYPSAILGRERAIVLNLEHIKAIITAEEVLIRDSSDENVIPVLEEFQRRLPVG 120

Query: 121 SSIY--QGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETDTYPAL 180
           +  +   G G+  E         E+E PFEFRALEVALEAICSFL ART ELE   YPAL
Sbjct: 121 NEAHGVHGDGDLGE---------EDESPFEFRALEVALEAICSFLAARTTELEKFAYPAL 180

Query: 181 DELTSKISSRNLDRVRKLKSAMTRLTNRVQKVVRDELEQLLDDDDDMAELYLSRKVAGMY 240
           DELT KISSRNL+RVRKLKSAMTRLT RVQK VRDELEQLLDDD DMA+LYL+RK+ G  
Sbjct: 181 DELTLKISSRNLERVRKLKSAMTRLTARVQK-VRDELEQLLDDDGDMADLYLTRKLVGAS 240

Query: 241 TPESGSGTPNWLLASPKDYYSKMSKTSRASLATIRG--ENDVEELEMLLEASSFILNVLQ 300
           +  S S  P W   SP    S +S+ SR SL T+RG  E DVEELEMLLE          
Sbjct: 241 SSVSVSDEPIWYPTSP-TIGSMISRASRVSLVTVRGDDETDVEELEMLLE---------- 300

Query: 301 AYFMQIESTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTA 360
           AYFMQI+STLNKL  LREYIDDTEDYINIQLDNHRNQLIQLEL LS+GTVC+S+YS++  
Sbjct: 301 AYFMQIDSTLNKLTELREYIDDTEDYINIQLDNHRNQLIQLELMLSAGTVCVSVYSMIAG 360

Query: 361 IFGMNIPYTWKEDHEHVFKWVVIVTGIVCASIYLTLSSYARYKGLVES 405
           IFGMNIP TW  DH ++FKWVV +TG  C  +++ + SYAR++GL+ S
Sbjct: 361 IFGMNIPNTWNHDHGYIFKWVVSLTGTFCIVLFVIILSYARFRGLIGS 386

BLAST of MS020311 vs. TAIR 10
Match: AT5G09690.2 (magnesium transporter 7 )

HSP 1 Score: 466.1 bits (1198), Expect = 2.9e-131
Identity = 262/408 (64.22%), Postives = 307/408 (75.25%), Query Frame = 0

Query: 1   MAGDAFLIPVESQASVKKKTKKKTVISRSWVSLDHEGRSTVLDVDKYAIMQRVQIHARDL 60
           M+ D  L+PV+S A V  K +K + +SRSW+S+D  G+ TVLDVDK+ IM RVQIHARDL
Sbjct: 12  MSPDGELVPVDSSAVVTAK-RKTSQLSRSWISIDATGQKTVLDVDKHVIMHRVQIHARDL 71

Query: 61  RLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEELQRRLPST 120
           R+LDP L YPS ILGRER IVLNLEHIK+IITA+EVL+RD  DENV+P++EE QRRLP  
Sbjct: 72  RILDPNLFYPSAILGRERAIVLNLEHIKAIITAEEVLIRDSSDENVIPVLEEFQRRLPVG 131

Query: 121 SSIY--QGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETDTYPAL 180
           +  +   G G+  E         E+E PFEFRALEVALEAICSFL ART ELE   YPAL
Sbjct: 132 NEAHGVHGDGDLGE---------EDESPFEFRALEVALEAICSFLAARTTELEKFAYPAL 191

Query: 181 DELTSKISSRNLDRVRKLKSAMTRLTNRVQKVVRDELEQLLDDDDDMAELYLSRKVAGMY 240
           DELT KISSRNL+RVRKLKSAMTRLT RVQK VRDELEQLLDDD DMA+LYL+RK+ G  
Sbjct: 192 DELTLKISSRNLERVRKLKSAMTRLTARVQK-VRDELEQLLDDDGDMADLYLTRKLVGAS 251

Query: 241 TPESGSGTPNWLLASPKDYYSKMSKTSRASLATIRG--ENDVEELEMLLEASSFILNVLQ 300
           +  S S  P W   SP    S +S+ SR SL T+RG  E DVEELEMLLE          
Sbjct: 252 SSVSVSDEPIWYPTSP-TIGSMISRASRVSLVTVRGDDETDVEELEMLLE---------- 311

Query: 301 AYFMQIESTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTA 360
           AYFMQI+STLNKL  LREYIDDTEDYINIQLDNHRNQLIQLEL LS+GTVC+S+YS++  
Sbjct: 312 AYFMQIDSTLNKLTELREYIDDTEDYINIQLDNHRNQLIQLELMLSAGTVCVSVYSMIAG 371

Query: 361 IFGMNIPYTWKEDHEHVFKWVVIVTGIVCASIYLTLSSYARYKGLVES 405
           IFGMNIP TW  DH ++FKWVV +TG  C  +++ + SYAR++GL+ S
Sbjct: 372 IFGMNIPNTWNHDHGYIFKWVVSLTGTFCIVLFVIILSYARFRGLIGS 397

BLAST of MS020311 vs. TAIR 10
Match: AT5G09690.4 (magnesium transporter 7 )

HSP 1 Score: 438.3 bits (1126), Expect = 6.5e-123
Identity = 250/408 (61.27%), Postives = 294/408 (72.06%), Query Frame = 0

Query: 1   MAGDAFLIPVESQASVKKKTKKKTVISRSWVSLDHEGRSTVLDVDKYAIMQRVQIHARDL 60
           M+ D  L+PV+S A V  K +K + +SRSW+S+D  G+ TVLDVDK+ IM RVQIHARDL
Sbjct: 1   MSPDGELVPVDSSAVVTAK-RKTSQLSRSWISIDATGQKTVLDVDKHVIMHRVQIHARDL 60

Query: 61  RLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEELQRRLPST 120
           R+LDP L YPS ILGRER IVLNLEHIK+IITA+EVL+RD  DENV+P++EE QRRLP  
Sbjct: 61  RILDPNLFYPSAILGRERAIVLNLEHIKAIITAEEVLIRDSSDENVIPVLEEFQRRLPVG 120

Query: 121 SSIY--QGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETDTYPAL 180
           +  +   G G+  E         E+E PFEFRALEVALEAICSFL ART ELE   YPAL
Sbjct: 121 NEAHGVHGDGDLGE---------EDESPFEFRALEVALEAICSFLAARTTELEKFAYPAL 180

Query: 181 DELTSKISSRNLDRVRKLKSAMTRLTNRVQKVVRDELEQLLDDDDDMAELYLSRKVAGMY 240
           DELT KISSRNL+RVRKLKSAMTRLT RVQK VRDELEQLLDDD DMA+LYL+RK+ G  
Sbjct: 181 DELTLKISSRNLERVRKLKSAMTRLTARVQK-VRDELEQLLDDDGDMADLYLTRKLVGAS 240

Query: 241 TPESGSGTPNWLLASPKDYYSKMSKTSRASLATIRG--ENDVEELEMLLEASSFILNVLQ 300
           +  S S  P W   SP    S +S+ SR SL T+RG  E DVEELEMLLE          
Sbjct: 241 SSVSVSDEPIWYPTSP-TIGSMISRASRVSLVTVRGDDETDVEELEMLLE---------- 300

Query: 301 AYFMQIESTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTA 360
                          LREYIDDTEDYINIQLDNHRNQLIQLEL LS+GTVC+S+YS++  
Sbjct: 301 ---------------LREYIDDTEDYINIQLDNHRNQLIQLELMLSAGTVCVSVYSMIAG 360

Query: 361 IFGMNIPYTWKEDHEHVFKWVVIVTGIVCASIYLTLSSYARYKGLVES 405
           IFGMNIP TW  DH ++FKWVV +TG  C  +++ + SYAR++GL+ S
Sbjct: 361 IFGMNIPNTWNHDHGYIFKWVVSLTGTFCIVLFVIILSYARFRGLIGS 371

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022146007.11.7e-21097.28magnesium transporter MRS2-I-like [Momordica charantia][more]
KAG6571485.11.1e-18888.65Magnesium transporter MRS2-7, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_023554091.12.4e-18888.19magnesium transporter MRS2-I-like isoform X1 [Cucurbita pepo subsp. pepo][more]
KAA0057420.12.4e-18888.51magnesium transporter MRS2-I-like isoform X2 [Cucumis melo var. makuwa] >TYK3011... [more]
XP_022963506.13.2e-18888.65magnesium transporter MRS2-I-like isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9FLG22.2e-13968.70Magnesium transporter MRS2-2 OS=Arabidopsis thaliana OX=3702 GN=MRS2-2 PE=2 SV=1[more]
B8AJT93.7e-13970.91Magnesium transporter MRS2-I OS=Oryza sativa subsp. indica OX=39946 GN=MRS2-I PE... [more]
Q10D383.7e-13970.91Magnesium transporter MRS2-I OS=Oryza sativa subsp. japonica OX=39947 GN=MRS2-I ... [more]
Q304A04.1e-13064.22Magnesium transporter MRS2-7 OS=Arabidopsis thaliana OX=3702 GN=MRS2-7 PE=2 SV=1[more]
P0CZ214.4e-10857.25Magnesium transporter MRS2-8 OS=Arabidopsis thaliana OX=3702 GN=MRS2-8 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CYA88.4e-21197.28Magnesium transporter OS=Momordica charantia OX=3673 GN=LOC111015319 PE=3 SV=1[more]
A0A5D3E2W61.2e-18888.51Magnesium transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21... [more]
A0A6J1HGA41.5e-18888.65Magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111463817 PE=3 SV=1[more]
A0A6J1HR332.6e-18888.81Magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111467011 PE=3 SV=1[more]
A0A0A0LLQ53.4e-18888.56Magnesium transporter OS=Cucumis sativus OX=3659 GN=Csa_2G225310 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G64560.11.5e-14068.70magnesium transporter 9 [more]
AT5G64560.24.5e-13269.90magnesium transporter 9 [more]
AT5G09690.12.9e-13164.22magnesium transporter 7 [more]
AT5G09690.22.9e-13164.22magnesium transporter 7 [more]
AT5G09690.46.5e-12361.27magnesium transporter 7 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 193..213
NoneNo IPR availableGENE3D1.20.58.340Magnesium transport protein CorA, transmembrane regioncoord: 104..334
e-value: 1.0E-61
score: 209.4
NoneNo IPR availableGENE3D1.20.58.340Magnesium transport protein CorA, transmembrane regioncoord: 341..395
e-value: 4.2E-5
score: 25.6
NoneNo IPR availableGENE3D2.40.128.330coord: 13..101
e-value: 3.7E-20
score: 73.2
NoneNo IPR availablePANTHERPTHR13890:SF46MAGNESIUM TRANSPORTER MRS2-LIKE PROTEINcoord: 5..404
NoneNo IPR availableSUPERFAMILY144083Magnesium transport protein CorA, transmembrane regioncoord: 340..391
IPR002523Mg2+ transporter protein, CorA-like/Zinc transport protein ZntBPFAMPF01544CorAcoord: 296..389
e-value: 1.5E-6
score: 27.8
IPR039204Magnesium transporter MRS2-likePANTHERPTHR13890RNA SPLICING PROTEIN MRS2, MITOCHONDRIALcoord: 5..404
IPR039204Magnesium transporter MRS2-likeCDDcd12823Mrs2_Mfm1p-likecoord: 30..401
e-value: 2.14225E-95
score: 286.833

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS020311.1MS020311.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030001 metal ion transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016020 membrane
molecular_function GO:0046873 metal ion transmembrane transporter activity