MS020295 (gene) Bitter gourd (TR) v1

Overview


Name	MS020295
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Proteinase inhibitor
Location	scaffold665: 800336 .. 800522 (-)
RNA-Seq Expression	MS020295
Synteny	MS020295

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TCATGGCCAGAACTGGTGTTTGTTGATGCAAGTGTTTGTGAGCAACATCATAAAGAAAGATCATCCTGATTTCAAGATAATTGTGCTGTTAGCAGGAACTCCAATTACCACAAAAGAATTGAGGTTCGACCGGGTTCGACTTTTTGTTAATATAAACAATGTTGTGGTTGAAACTCCCATCAATGGT

mRNA sequence

TCATGGCCAGAACTGGTGTTTGTTGATGCAAGTGTGAGCAACATCATAAAGAAAGATCATCCTGATTTCAAGATAATTGTGCTGTTAGCAGGAACTCCAATTACCACAAAAGAATTGAGGTTCGACCGGGTTCGACTTTTTGTTAATATAAACAATGTTGTGGTTGAAACTCCCATCAATGGT

Coding sequence (CDS)

TCATGGCCAGAACTGGTGTTTGTTGATGCAAGTGTGAGCAACATCATAAAGAAAGATCATCCTGATTTCAAGATAATTGTGCTGTTAGCAGGAACTCCAATTACCACAAAAGAATTGAGGTTCGACCGGGTTCGACTTTTTGTTAATATAAACAATGTTGTGGTTGAAACTCCCATCAATGGT

Protein sequence

SWPELVFVDASVSNIIKKDHPDFKIIVLLAGTPITTKELRFDRVRLFVNINNVVVETPING

Homology

[Close]

BLAST of MS020295 vs. NCBI nr
Match: KAG6571807.1 (hypothetical protein SDJN03_28535, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 73.2 bits (178), Expect = 8.9e-10
Identity = 37/62 (59.68%), Postives = 48/62 (77.42%), Query Frame = 0


Query: 1  SWPELVFVDAS-VSNIIKKDHPDFKIIVLLAGTPITTKELRFDRVRLFVNINNVVVETPI 60
          SWPEL  + AS V NIIK+ HP+F+I++LLAG+P+ TK+L+  RVRLFVNI  +VVE P 
Sbjct: 7  SWPELNLMQASTVENIIKRQHPEFQIVILLAGSPV-TKDLKHGRVRLFVNILGIVVEIPR 66

Query: 61 NG 62
           G
Sbjct: 67 EG 67

[Close]

BLAST of MS020295 vs. NCBI nr
Match: KGN60477.1 (hypothetical protein Csa_001998 [Cucumis sativus])

HSP 1 Score: 70.1 bits (170), Expect = 7.5e-09
Identity = 35/62 (56.45%), Postives = 48/62 (77.42%), Query Frame = 0


Query: 1  SWPELVFVDA-SVSNIIKKDHPDFKIIVLLAGTPITTKELRFDRVRLFVNINNVVVETPI 60
          SWP L F+DA +V+NIIKK++PDF+I +LLAG+P+ TK+L+  RVRLF N+  +VV  P 
Sbjct: 11 SWPALNFMDARTVANIIKKENPDFQIFILLAGSPV-TKDLKHGRVRLFTNMEEIVVIVPQ 70

Query: 61 NG 62
           G
Sbjct: 71 EG 71

[Close]

BLAST of MS020295 vs. NCBI nr
Match: XP_011654473.1 (inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetical protein Csa_018430 [Cucumis sativus])

HSP 1 Score: 68.6 bits (166), Expect = 2.2e-08
Identity = 36/62 (58.06%), Postives = 50/62 (80.65%), Query Frame = 0


Query: 1  SWPELVFVDASVS-NIIKKDHPDFKIIVLLAGTPITTKELRFDRVRLFVNINNVVVETPI 60
          SWPELVFV +SV+ + I++D PD K +VLLAG+P+ T++LR +RVR+FVN+N+ VVE P 
Sbjct: 15 SWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPV-TEDLRPNRVRIFVNMNDRVVEVPR 74

Query: 61 NG 62
           G
Sbjct: 75 TG 75

[Close]

BLAST of MS020295 vs. NCBI nr
Match: ALD47589.1 (serine proteinase inhibitor [Cucumis metulifer])

HSP 1 Score: 59.3 bits (142), Expect = 1.3e-05
Identity = 35/59 (59.32%), Postives = 43/59 (72.88%), Query Frame = 0


Query: 2  WPELVFVDASVSN-IIKKDHPDF-KIIVLLAGTPITTKELRFDRVRLFVNINNVVVETP 59
          WPELV + A+ +  II+KD+P   K+IVLLAGT  TTK++R DRV LFVNI   VVE P
Sbjct: 12 WPELVGIKATTAEYIIRKDNPSVEKVIVLLAGTG-TTKDIRCDRVWLFVNIEEKVVEIP 69

[Close]

BLAST of MS020295 vs. NCBI nr
Match: KGN50838.1 (hypothetical protein Csa_004692 [Cucumis sativus])

HSP 1 Score: 58.9 bits (141), Expect = 1.7e-05
Identity = 35/62 (56.45%), Postives = 45/62 (72.58%), Query Frame = 0


Query: 2  WPELVFVDASVSN-IIKKDHPDFK-IIVLLAGTPITTKELRFDRVRLFVNINNVVVETPI 61
          WPELV + A+++  IIKKD+P+ + +IVLLAGT  TTK+ R DRV LF NI+  VVE P 
Sbjct: 12 WPELVGIKATIAEYIIKKDNPNVENVIVLLAGTG-TTKDFRSDRVWLFTNIDGRVVEMPT 71

[Close]

BLAST of MS020295 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 4.7e-06
Identity = 27/59 (45.76%), Postives = 40/59 (67.80%), Query Frame = 0


Query: 1  SWPELVFVDASVSN-IIKKDHPDFKIIVLLAGTPITTKELRFDRVRLFVNINNVVVETP 59
          SWP LV V  SV+  II++ +P+ K ++L  GTP+ TK+ R +RVR++VN   +VV  P
Sbjct: 8  SWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPV-TKDFRCNRVRIWVNKRGLVVSPP 65

[Close]

BLAST of MS020295 vs. ExPASy Swiss-Prot
Match: Q6XNP7 (Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2)

HSP 1 Score: 49.7 bits (117), Expect = 1.4e-05
Identity = 27/62 (43.55%), Postives = 41/62 (66.13%), Query Frame = 0


Query: 1  SWPELVFVDASV-SNIIKKDHPDFKIIVLLAGTPITTKELRFDRVRLFVNINNVVVETPI 60
          SWPELV  +  + + II+ ++ + K IV+  G PI T++L F+RVR+FV+ N VV + P 
Sbjct: 10 SWPELVGTNGDIAAGIIQTENANVKAIVVKEGLPI-TQDLNFNRVRVFVDENRVVTQVPA 69

Query: 61 NG 62
           G
Sbjct: 70 IG 70

[Close]

BLAST of MS020295 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 1.4e-05
Identity = 30/59 (50.85%), Postives = 39/59 (66.10%), Query Frame = 0


Query: 2   WPELVFVDA-SVSNIIKKDHPDF-KIIVLLAGTPITTKELRFDRVRLFVNINNVVVETP 59
           WPEL+ V A     II+K++P    I +LL G+P+ TK+ R DRVRLFVNI   VV+ P
Sbjct: 51  WPELIGVPALYAKGIIEKENPSITNIPILLNGSPV-TKDFRCDRVRLFVNILGDVVQIP 108

[Close]

BLAST of MS020295 vs. ExPASy Swiss-Prot
Match: P20076 (Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 PE=3 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 5.3e-05
Identity = 25/59 (42.37%), Postives = 37/59 (62.71%), Query Frame = 0


Query: 1   SWPELVFVDAS-VSNIIKKDHPDFKIIVLLAGTPITTKELRFDRVRLFVNINNVVVETP 59
           SWPEL+   A     II+K++P    +  L      T++LR +RVRLFVN+ ++VV+TP
Sbjct: 58  SWPELLGTPAKFAKQIIQKENPKLTNVETLLNGSAFTEDLRCNRVRLFVNLLDIVVQTP 116

[Close]

BLAST of MS020295 vs. ExPASy Swiss-Prot
Match: Q03199 (Proteinase inhibitor I-B OS=Nicotiana tabacum OX=4097 GN=TIMPA PE=2 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 5.3e-05
Identity = 27/59 (45.76%), Postives = 41/59 (69.49%), Query Frame = 0


Query: 2   WPELVFVDASVS-NIIKKDHPDF-KIIVLLAGTPITTKELRFDRVRLFVNINNVVVETP 59
           WPEL+   A  +  II+K++P    +  +L GTP+ T++LR +RVRLFVN+ + VV+TP
Sbjct: 47  WPELLGTPAKFAMQIIQKENPKLTNVQTVLNGTPV-TEDLRCNRVRLFVNVLDFVVQTP 104

[Close]

BLAST of MS020295 vs. ExPASy TrEMBL
Match: A0A0A0LIF6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914570 PE=3 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 3.6e-09
Identity = 35/62 (56.45%), Postives = 48/62 (77.42%), Query Frame = 0


Query: 1  SWPELVFVDA-SVSNIIKKDHPDFKIIVLLAGTPITTKELRFDRVRLFVNINNVVVETPI 60
          SWP L F+DA +V+NIIKK++PDF+I +LLAG+P+ TK+L+  RVRLF N+  +VV  P 
Sbjct: 11 SWPALNFMDARTVANIIKKENPDFQIFILLAGSPV-TKDLKHGRVRLFTNMEEIVVIVPQ 70

Query: 61 NG 62
           G
Sbjct: 71 EG 71

[Close]

BLAST of MS020295 vs. ExPASy TrEMBL
Match: A0A0A0KPI0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.1e-08
Identity = 36/62 (58.06%), Postives = 50/62 (80.65%), Query Frame = 0


Query: 1  SWPELVFVDASVS-NIIKKDHPDFKIIVLLAGTPITTKELRFDRVRLFVNINNVVVETPI 60
          SWPELVFV +SV+ + I++D PD K +VLLAG+P+ T++LR +RVR+FVN+N+ VVE P 
Sbjct: 15 SWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPV-TEDLRPNRVRIFVNMNDRVVEVPR 74

Query: 61 NG 62
           G
Sbjct: 75 TG 75

[Close]

BLAST of MS020295 vs. ExPASy TrEMBL
Match: A0A0M3SAG8 (Serine proteinase inhibitor OS=Cucumis metulifer OX=61886 PE=2 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 6.4e-06
Identity = 35/59 (59.32%), Postives = 43/59 (72.88%), Query Frame = 0


Query: 2  WPELVFVDASVSN-IIKKDHPDF-KIIVLLAGTPITTKELRFDRVRLFVNINNVVVETP 59
          WPELV + A+ +  II+KD+P   K+IVLLAGT  TTK++R DRV LFVNI   VVE P
Sbjct: 12 WPELVGIKATTAEYIIRKDNPSVEKVIVLLAGTG-TTKDIRCDRVWLFVNIEEKVVEIP 69

[Close]

BLAST of MS020295 vs. ExPASy TrEMBL
Match: A0A0A0KMM0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G285030 PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 8.4e-06
Identity = 35/62 (56.45%), Postives = 45/62 (72.58%), Query Frame = 0


Query: 2  WPELVFVDASVSN-IIKKDHPDFK-IIVLLAGTPITTKELRFDRVRLFVNINNVVVETPI 61
          WPELV + A+++  IIKKD+P+ + +IVLLAGT  TTK+ R DRV LF NI+  VVE P 
Sbjct: 12 WPELVGIKATIAEYIIKKDNPNVENVIVLLAGTG-TTKDFRSDRVWLFTNIDGRVVEMPT 71

[Close]

BLAST of MS020295 vs. ExPASy TrEMBL
Match: A0A0A0KME7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286040 PE=3 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 2.4e-05
Identity = 32/59 (54.24%), Postives = 47/59 (79.66%), Query Frame = 0


Query: 2  WPELVFVDASVSN-IIKKDHPDFK-IIVLLAGTPITTKELRFDRVRLFVNINNVVVETP 59
          WPELV V A+ +  IIKKD+P+ + ++VLLAG+  TT+++R DRV +FVNI+++VVE P
Sbjct: 12 WPELVGVKATTAKYIIKKDNPNVENVVVLLAGSG-TTEDIRCDRVWVFVNIHDLVVEVP 69

[Close]

BLAST of MS020295 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 49.7 bits (117), Expect = 9.8e-07
Identity = 25/62 (40.32%), Postives = 41/62 (66.13%), Query Frame = 0


Query: 1  SWPELVFVDAS-VSNIIKKDHPDFKIIVLLAGTPITTKELRFDRVRLFVNINNVVVETPI 60
          SWPEL   +    + +I++++P     V+L G+P+T  + R DRVR+FV+ N +VV+TP 
Sbjct: 10 SWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTA-DFRCDRVRVFVDGNRIVVKTPK 69

Query: 61 NG 62
          +G
Sbjct: 70 SG 70

The following BLAST results are available for this feature:

BLAST of MS020295 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6571807.1	8.9e-10	59.68	hypothetical protein SDJN03_28535, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KGN60477.1	7.5e-09	56.45	hypothetical protein Csa_001998 [Cucumis sativus]	[more]
XP_011654473.1	2.2e-08	58.06	inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetic...	[more]
ALD47589.1	1.3e-05	59.32	serine proteinase inhibitor [Cucumis metulifer]	[more]
KGN50838.1	1.7e-05	56.45	hypothetical protein Csa_004692 [Cucumis sativus]	[more]

BLAST of MS020295 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P19873	4.7e-06	45.76	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
Q6XNP7	1.4e-05	43.55	Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2	[more]
P16231	1.4e-05	50.85	Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1	[more]
P20076	5.3e-05	42.37	Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 PE=3 ...	[more]
Q03199	5.3e-05	45.76	Proteinase inhibitor I-B OS=Nicotiana tabacum OX=4097 GN=TIMPA PE=2 SV=1	[more]

BLAST of MS020295 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LIF6	3.6e-09	56.45	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914570 PE=3 SV=1	[more]
A0A0A0KPI0	1.1e-08	58.06	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1	[more]
A0A0M3SAG8	6.4e-06	59.32	Serine proteinase inhibitor OS=Cucumis metulifer OX=61886 PE=2 SV=1	[more]
A0A0A0KMM0	8.4e-06	56.45	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G285030 PE=3 SV=1	[more]
A0A0A0KME7	2.4e-05	54.24	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286040 PE=3 SV=1	[more]

BLAST of MS020295 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38870.1	9.8e-07	40.32	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 1..60 e-value: 3.1E-14 score: 53.4
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 1..60
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 1..61 e-value: 5.4E-15 score: 57.5
None	No IPR available	PANTHER	PTHR33091:SF83	ETHYLENE-RESPONSIVE PROTEINASE INHIBITOR 1	coord: 1..60
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 1..61

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS020295.1	MS020295.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity