MS020267 (gene) Bitter gourd (TR) v1

Overview
NameMS020267
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationscaffold665: 582504 .. 584498 (-)
RNA-Seq ExpressionMS020267
SyntenyMS020267
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCAACTATGGAAGATCTCAGTTCCTCGTAGATTCTTAGTGAAGCGCAACATTTTTGCTCCATTTACCTTTCCTCTCACGAGGACTTCAAATTACTTGATTCTTCGTGGCCTCCATACTCAGACCCACACTTCCTCGTTGCTTCCACCCTCGCTGTCTTCATTTTCGTTGCTTCCTAATATTATTGAATTATTCTCTAACAAGCCTTCCGATCATGACCTTCTGGTGAGAATGAATATGCAACGGAAGTTAACGGAGGTGCGGCACGAGCTGATTCAGAATGCCGACGATTCCGATAAGATCGTGAAAAAATTGGAAGACTGTGAAGACTTGCTCTTACGGAGACATGCCGATGGTTCTGCTTTTGTTGAACTGCTGAAGAAGTTGGATTCACGGCCTCAATTAGCACTGGAGGTGAGTTTAATTCTACATGCTCAAAGGAAATTGCTTCATATGTTGTTTCTATGATTATATGCTTTATGTGGGGATTGCTTACGGTTGTAGTCAGTATTAAATGGTTAATGTTATTGTTTTAGTTCTGTCTTACATGGATTATTTTTCCTTCTTTGTACTAAATGTTGAGGTCAAGAACATCTAGATGATAATTCAAGTGAAATAAAATTACTGGGATTGTTGGGCAATGACTAAGATGCTGCTGTTAACTTTGAGTTTAGTTATTCATATTTACGGAAGGTTGAAATTTCATTGAAGCGGAATTATTACTTTTGATGCTTCATATTCAATATTGTTATGACTGTAAGTTGCATCACTGAATCCCCATTTGATTACAAATAACTTGGTAGTCATGTACAAGACCTTGGTAAACAATTTGATGAAACCAAACTTCTGATCAGCCTATTCTTTTAAACTTAGGTGTTTAATTGGAGAAGAAGGCAAGAGGGTTCCTTTCCTTTGACTGCAGAGGAATATGCTAAGGGTATTACAGTTGCAGGTAGATCTAAAAATGTTGACCTTGCAATTGAGTTGTTCACTGAGGCTTCTAATAGAAGGATCAAGACAACCTCTACTTACAACGCACTGATGGGAGCTTACATGTTTAATGGCTTGGTTGACAAATGCCACTCATTGTTTAGGGACCTCAAGAGGGAGGCGAATTGTGTTCCTACTATTGTAACATATAACATCCTAATATCAGTATTTGGTCGTTTGATGTTAGTTGATCACATGGAAGCAACCTTGCAAGAGATACGTGATTTGAAGCTCTCCCCCAATGTGAGCACGTACAATAACCTAATTGCTGGGTATGTTACTGCATGGATGTGGGATAGAATGGAGCAAACCTTCCTGACAATGAAGGCGAGTTCAGTAAATCCCAACACAGAGACTTTTTCACTAATGCTTCGTGGATATGCACATTCTGGTAATCTTGAGAAGATGGAAGAGATGTATGAATTGGTTAGAGGTCATGTCAGTAACAAGAATTTTCCTCTTATTAGGGCTATGATATGTGCATACTGTTGGAGTTCTATTGCAGATAGAGTGAAAAGGATTGAGGCTTTGTTGAAGCTTATTCCAGAAGAAGAATATAGACCATGGTTGAATGTCTTGTTGATTAGAGTCTATGCCCAAGAAGATTGGTTAGAGGGAATGGAGAGTTCAATAAATGAGGCATTTGAGCATGGTACTTCAGTAACTACTGTCCACGTGATGCGCTCCATTATTGCCAGCTATTTTCGGTGTAATGCAGTGGACAAGCTAACAAACTTTGTTAAGCGTGCTGAATGTTCAGGTTGGAGAATCTGTCGGTCTTTATATCATTGTAAAATGGTTATGTTTGCATCGCAGAAGCGCCTTGAAGAAATGGAGTGCGTCCTAAATGAAATGAAAAGTATCAACTTGGACTGGACAAAAAAAACATTTTGGATTTTATATAAGGCCTACTCAGTCAGTGGTTGCAGGTACAAGGTGGACCAAGTGGTAGGTTTGATGTGCAAGCTTGGATATGGGATTCCCTTGGATTCTTGTCCATCT

mRNA sequence

ATGAAGCAACTATGGAAGATCTCAGTTCCTCGTAGATTCTTAGTGAAGCGCAACATTTTTGCTCCATTTACCTTTCCTCTCACGAGGACTTCAAATTACTTGATTCTTCGTGGCCTCCATACTCAGACCCACACTTCCTCGTTGCTTCCACCCTCGCTGTCTTCATTTTCGTTGCTTCCTAATATTATTGAATTATTCTCTAACAAGCCTTCCGATCATGACCTTCTGGTGAGAATGAATATGCAACGGAAGTTAACGGAGGTGCGGCACGAGCTGATTCAGAATGCCGACGATTCCGATAAGATCGTGAAAAAATTGGAAGACTGTGAAGACTTGCTCTTACGGAGACATGCCGATGGTTCTGCTTTTGTTGAACTGCTGAAGAAGTTGGATTCACGGCCTCAATTAGCACTGGAGGTGTTTAATTGGAGAAGAAGGCAAGAGGGTTCCTTTCCTTTGACTGCAGAGGAATATGCTAAGGGTATTACAGTTGCAGGTAGATCTAAAAATGTTGACCTTGCAATTGAGTTGTTCACTGAGGCTTCTAATAGAAGGATCAAGACAACCTCTACTTACAACGCACTGATGGGAGCTTACATGTTTAATGGCTTGGTTGACAAATGCCACTCATTGTTTAGGGACCTCAAGAGGGAGGCGAATTGTGTTCCTACTATTGTAACATATAACATCCTAATATCAGTATTTGGTCGTTTGATGTTAGTTGATCACATGGAAGCAACCTTGCAAGAGATACGTGATTTGAAGCTCTCCCCCAATGTGAGCACGTACAATAACCTAATTGCTGGGTATGTTACTGCATGGATGTGGGATAGAATGGAGCAAACCTTCCTGACAATGAAGGCGAGTTCAGTAAATCCCAACACAGAGACTTTTTCACTAATGCTTCGTGGATATGCACATTCTGGTAATCTTGAGAAGATGGAAGAGATGTATGAATTGGTTAGAGGTCATGTCAGTAACAAGAATTTTCCTCTTATTAGGGCTATGATATGTGCATACTGTTGGAGTTCTATTGCAGATAGAGTGAAAAGGATTGAGGCTTTGTTGAAGCTTATTCCAGAAGAAGAATATAGACCATGGTTGAATGTCTTGTTGATTAGAGTCTATGCCCAAGAAGATTGGTTAGAGGGAATGGAGAGTTCAATAAATGAGGCATTTGAGCATGGTACTTCAGTAACTACTGTCCACGTGATGCGCTCCATTATTGCCAGCTATTTTCGGTGTAATGCAGTGGACAAGCTAACAAACTTTGTTAAGCGTGCTGAATGTTCAGGTTGGAGAATCTGTCGGTCTTTATATCATTGTAAAATGGTTATGTTTGCATCGCAGAAGCGCCTTGAAGAAATGGAGTGCGTCCTAAATGAAATGAAAAGTATCAACTTGGACTGGACAAAAAAAACATTTTGGATTTTATATAAGGCCTACTCAGTCAGTGGTTGCAGGTACAAGGTGGACCAAGTGGTAGGTTTGATGTGCAAGCTTGGATATGGGATTCCCTTGGATTCTTGTCCATCT

Coding sequence (CDS)

ATGAAGCAACTATGGAAGATCTCAGTTCCTCGTAGATTCTTAGTGAAGCGCAACATTTTTGCTCCATTTACCTTTCCTCTCACGAGGACTTCAAATTACTTGATTCTTCGTGGCCTCCATACTCAGACCCACACTTCCTCGTTGCTTCCACCCTCGCTGTCTTCATTTTCGTTGCTTCCTAATATTATTGAATTATTCTCTAACAAGCCTTCCGATCATGACCTTCTGGTGAGAATGAATATGCAACGGAAGTTAACGGAGGTGCGGCACGAGCTGATTCAGAATGCCGACGATTCCGATAAGATCGTGAAAAAATTGGAAGACTGTGAAGACTTGCTCTTACGGAGACATGCCGATGGTTCTGCTTTTGTTGAACTGCTGAAGAAGTTGGATTCACGGCCTCAATTAGCACTGGAGGTGTTTAATTGGAGAAGAAGGCAAGAGGGTTCCTTTCCTTTGACTGCAGAGGAATATGCTAAGGGTATTACAGTTGCAGGTAGATCTAAAAATGTTGACCTTGCAATTGAGTTGTTCACTGAGGCTTCTAATAGAAGGATCAAGACAACCTCTACTTACAACGCACTGATGGGAGCTTACATGTTTAATGGCTTGGTTGACAAATGCCACTCATTGTTTAGGGACCTCAAGAGGGAGGCGAATTGTGTTCCTACTATTGTAACATATAACATCCTAATATCAGTATTTGGTCGTTTGATGTTAGTTGATCACATGGAAGCAACCTTGCAAGAGATACGTGATTTGAAGCTCTCCCCCAATGTGAGCACGTACAATAACCTAATTGCTGGGTATGTTACTGCATGGATGTGGGATAGAATGGAGCAAACCTTCCTGACAATGAAGGCGAGTTCAGTAAATCCCAACACAGAGACTTTTTCACTAATGCTTCGTGGATATGCACATTCTGGTAATCTTGAGAAGATGGAAGAGATGTATGAATTGGTTAGAGGTCATGTCAGTAACAAGAATTTTCCTCTTATTAGGGCTATGATATGTGCATACTGTTGGAGTTCTATTGCAGATAGAGTGAAAAGGATTGAGGCTTTGTTGAAGCTTATTCCAGAAGAAGAATATAGACCATGGTTGAATGTCTTGTTGATTAGAGTCTATGCCCAAGAAGATTGGTTAGAGGGAATGGAGAGTTCAATAAATGAGGCATTTGAGCATGGTACTTCAGTAACTACTGTCCACGTGATGCGCTCCATTATTGCCAGCTATTTTCGGTGTAATGCAGTGGACAAGCTAACAAACTTTGTTAAGCGTGCTGAATGTTCAGGTTGGAGAATCTGTCGGTCTTTATATCATTGTAAAATGGTTATGTTTGCATCGCAGAAGCGCCTTGAAGAAATGGAGTGCGTCCTAAATGAAATGAAAAGTATCAACTTGGACTGGACAAAAAAAACATTTTGGATTTTATATAAGGCCTACTCAGTCAGTGGTTGCAGGTACAAGGTGGACCAAGTGGTAGGTTTGATGTGCAAGCTTGGATATGGGATTCCCTTGGATTCTTGTCCATCT

Protein sequence

MKQLWKISVPRRFLVKRNIFAPFTFPLTRTSNYLILRGLHTQTHTSSLLPPSLSSFSLLPNIIELFSNKPSDHDLLVRMNMQRKLTEVRHELIQNADDSDKIVKKLEDCEDLLLRRHADGSAFVELLKKLDSRPQLALEVFNWRRRQEGSFPLTAEEYAKGITVAGRSKNVDLAIELFTEASNRRIKTTSTYNALMGAYMFNGLVDKCHSLFRDLKREANCVPTIVTYNILISVFGRLMLVDHMEATLQEIRDLKLSPNVSTYNNLIAGYVTAWMWDRMEQTFLTMKASSVNPNTETFSLMLRGYAHSGNLEKMEEMYELVRGHVSNKNFPLIRAMICAYCWSSIADRVKRIEALLKLIPEEEYRPWLNVLLIRVYAQEDWLEGMESSINEAFEHGTSVTTVHVMRSIIASYFRCNAVDKLTNFVKRAECSGWRICRSLYHCKMVMFASQKRLEEMECVLNEMKSINLDWTKKTFWILYKAYSVSGCRYKVDQVVGLMCKLGYGIPLDSCPS
Homology
BLAST of MS020267 vs. NCBI nr
Match: XP_022145941.1 (pentatricopeptide repeat-containing protein At2g30780 [Momordica charantia] >XP_022145942.1 pentatricopeptide repeat-containing protein At2g30780 [Momordica charantia] >XP_022145943.1 pentatricopeptide repeat-containing protein At2g30780 [Momordica charantia] >XP_022145944.1 pentatricopeptide repeat-containing protein At2g30780 [Momordica charantia] >XP_022145945.1 pentatricopeptide repeat-containing protein At2g30780 [Momordica charantia] >XP_022145946.1 pentatricopeptide repeat-containing protein At2g30780 [Momordica charantia] >XP_022145947.1 pentatricopeptide repeat-containing protein At2g30780 [Momordica charantia] >XP_022145948.1 pentatricopeptide repeat-containing protein At2g30780 [Momordica charantia])

HSP 1 Score: 1028.1 bits (2657), Expect = 2.6e-296
Identity = 509/512 (99.41%), Postives = 510/512 (99.61%), Query Frame = 0

Query: 1   MKQLWKISVPRRFLVKRNIFAPFTFPLTRTSNYLILRGLHTQTHTSSLLPPSLSSFSLLP 60
           MKQLWKISVPRRFLVKRNIFAPFTFPLTRTSNYLILRGLHTQTHTSSLLPPSLSSFSLLP
Sbjct: 1   MKQLWKISVPRRFLVKRNIFAPFTFPLTRTSNYLILRGLHTQTHTSSLLPPSLSSFSLLP 60

Query: 61  NIIELFSNKPSDHDLLVRMNMQRKLTEVRHELIQNADDSDKIVKKLEDCEDLLLRRHADG 120
           NIIELFSNKPSDHDLLVRMNMQRKLTEVRHELIQNADDSDKIVKKLEDCEDLLLRRHADG
Sbjct: 61  NIIELFSNKPSDHDLLVRMNMQRKLTEVRHELIQNADDSDKIVKKLEDCEDLLLRRHADG 120

Query: 121 SAFVELLKKLDSRPQLALEVFNWRRRQEGSFPLTAEEYAKGITVAGRSKNVDLAIELFTE 180
           SAFVELLKKLDSRP LALEVFNWRRRQEGSFP+TAEEYAKGITVAGRSKNVDLAIELFTE
Sbjct: 121 SAFVELLKKLDSRPHLALEVFNWRRRQEGSFPMTAEEYAKGITVAGRSKNVDLAIELFTE 180

Query: 181 ASNRRIKTTSTYNALMGAYMFNGLVDKCHSLFRDLKREANCVPTIVTYNILISVFGRLML 240
           ASNRRIKTTSTYNALMGAYMFNGLVDKCHSLFRDLKREANCVPTIVTYNILISVFGRLML
Sbjct: 181 ASNRRIKTTSTYNALMGAYMFNGLVDKCHSLFRDLKREANCVPTIVTYNILISVFGRLML 240

Query: 241 VDHMEATLQEIRDLKLSPNVSTYNNLIAGYVTAWMWDRMEQTFLTMKASSVNPNTETFSL 300
           VDHMEATLQEIRDLKLSPNVSTYNNLIAGYVTAWMWDRMEQTFLTMKASSV PNTETFSL
Sbjct: 241 VDHMEATLQEIRDLKLSPNVSTYNNLIAGYVTAWMWDRMEQTFLTMKASSVKPNTETFSL 300

Query: 301 MLRGYAHSGNLEKMEEMYELVRGHVSNKNFPLIRAMICAYCWSSIADRVKRIEALLKLIP 360
           MLRGYAHSGNLEKMEEMYELVRGHVSNKNFPLIRAMICAYCWSSIADRVKRIEALLKLIP
Sbjct: 301 MLRGYAHSGNLEKMEEMYELVRGHVSNKNFPLIRAMICAYCWSSIADRVKRIEALLKLIP 360

Query: 361 EEEYRPWLNVLLIRVYAQEDWLEGMESSINEAFEHGTSVTTVHVMRSIIASYFRCNAVDK 420
           EEEYRPWLNVLLIRVYAQEDWLEGMESSINEAFEHGTSVTTVHVMRSIIASYFRCNAVDK
Sbjct: 361 EEEYRPWLNVLLIRVYAQEDWLEGMESSINEAFEHGTSVTTVHVMRSIIASYFRCNAVDK 420

Query: 421 LTNFVKRAECSGWRICRSLYHCKMVMFASQKRLEEMECVLNEMKSINLDWTKKTFWILYK 480
           LTNFVKRAECSGWRICRSLYHCKMVMFASQKRLEEMECVLNEMKSINLDWTKKTFWILYK
Sbjct: 421 LTNFVKRAECSGWRICRSLYHCKMVMFASQKRLEEMECVLNEMKSINLDWTKKTFWILYK 480

Query: 481 AYSVSGCRYKVDQVVGLMCKLGYGIPLDSCPS 513
           AYSVSGCRYKVDQVVGLMCKLGYGIPLDSCPS
Sbjct: 481 AYSVSGCRYKVDQVVGLMCKLGYGIPLDSCPS 512

BLAST of MS020267 vs. NCBI nr
Match: XP_022923507.1 (pentatricopeptide repeat-containing protein At2g30780 [Cucurbita moschata] >XP_022923508.1 pentatricopeptide repeat-containing protein At2g30780 [Cucurbita moschata])

HSP 1 Score: 851.7 bits (2199), Expect = 3.4e-243
Identity = 425/513 (82.85%), Postives = 458/513 (89.28%), Query Frame = 0

Query: 1   MKQLWKISVPRRFLVKRNIFAPFTFPLTRTSNYLIL-RGLHTQTHTSSLLPPSLSSFSLL 60
           MKQLWK SV  RFLV RNIFAPF+F L   SN+ I   GLHTQ+ +SS   PS+SSFSLL
Sbjct: 1   MKQLWKFSVRHRFLVNRNIFAPFSFSLANNSNHFIFPHGLHTQSCSSSSPVPSISSFSLL 60

Query: 61  PNIIELFSNKPSDHDLLVRMNMQRKLTEVRHELIQNADDSDKIVKKLEDCEDLLLRRHAD 120
           PNIIELFSNKPSD DLLVR  MQRK+TEV HELIQNA+DSDKIVK LED E  LLRRHAD
Sbjct: 61  PNIIELFSNKPSDQDLLVRSIMQRKVTEVMHELIQNAEDSDKIVKVLEDNEGFLLRRHAD 120

Query: 121 GSAFVELLKKLDSRPQLALEVFNWRRRQEGSFPLTAEEYAKGITVAGRSKNVDLAIELFT 180
            SAFVELLK+L S+P LALEVFNWRRRQEGSFPLTAEEYAK IT+AGRSKNVDLA+ELFT
Sbjct: 121 SSAFVELLKQLGSQPHLALEVFNWRRRQEGSFPLTAEEYAKSITLAGRSKNVDLAVELFT 180

Query: 181 EASNRRIKTTSTYNALMGAYMFNGLVDKCHSLFRDLKREANCVPTIVTYNILISVFGRLM 240
           EASN+R+KTTS YNALMGAYM+NGL DKCHSLFRDLKR+ANCVPTIVTYNIL+SVFGRLM
Sbjct: 181 EASNKRVKTTSIYNALMGAYMYNGLADKCHSLFRDLKRDANCVPTIVTYNILLSVFGRLM 240

Query: 241 LVDHMEATLQEIRDLKLSPNVSTYNNLIAGYVTAWMWDRMEQTFLTMKASSVNPNTETFS 300
           LVDHMEATL+EI DL LSPNVSTYN+LIAGYVTAWMWD+MEQTFL MKASSV PNTET  
Sbjct: 241 LVDHMEATLREIHDLDLSPNVSTYNHLIAGYVTAWMWDKMEQTFLKMKASSVKPNTETLL 300

Query: 301 LMLRGYAHSGNLEKMEEMYELVRGHVSNKNFPLIRAMICAYCWSSIADRVKRIEALLKLI 360
           LMLRGYAHS NLEKMEEM+EL + HV +KNFPLIR MICAYC S+IAD+V +IEALLKLI
Sbjct: 301 LMLRGYAHSDNLEKMEEMHELSKDHVDDKNFPLIRTMICAYCRSTIADKVTKIEALLKLI 360

Query: 361 PEEEYRPWLNVLLIRVYAQEDWLEGMESSINEAFEHGTSVTTVHVMRSIIASYFRCNAVD 420
           P+EEYRPWLNVLLI+VYAQEDWLE ME+SINEAFEHGTSV T+HVMRSI ASYFRCNAVD
Sbjct: 361 PKEEYRPWLNVLLIKVYAQEDWLERMENSINEAFEHGTSVNTLHVMRSISASYFRCNAVD 420

Query: 421 KLTNFVKRAECSGWRICRSLYHCKMVMFASQKRLEEMECVLNEMKSINLDWTKKTFWILY 480
           KLTNFV RAECSGWRICRSLYHCKMVMFAS+KR EEME VL+EMK+INLDWTKKTFWILY
Sbjct: 421 KLTNFVSRAECSGWRICRSLYHCKMVMFASEKRFEEMESVLDEMKNINLDWTKKTFWILY 480

Query: 481 KAYSVSGCRYKVDQVVGLMCKLGYGIPLDSCPS 513
           KAYS+SGCRYKVDQV   MCKLGYGIP D  PS
Sbjct: 481 KAYSLSGCRYKVDQVSCYMCKLGYGIPSDLSPS 513

BLAST of MS020267 vs. NCBI nr
Match: KAG7015626.1 (Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 848.6 bits (2191), Expect = 2.8e-242
Identity = 424/513 (82.65%), Postives = 458/513 (89.28%), Query Frame = 0

Query: 1   MKQLWKISVPRRFLVKRNIFAPFTFPLTRTSNYLIL-RGLHTQTHTSSLLPPSLSSFSLL 60
           MKQLWK SV  RFLV RNIFAPF+F L   SN+LI   GLHTQ+ +SS    S+SSFSLL
Sbjct: 1   MKQLWKFSVRHRFLVNRNIFAPFSFSLANNSNHLIFPHGLHTQSCSSSSPVQSISSFSLL 60

Query: 61  PNIIELFSNKPSDHDLLVRMNMQRKLTEVRHELIQNADDSDKIVKKLEDCEDLLLRRHAD 120
           PNIIELFSNKPSD DLLVR  MQRK+TEV HELIQNA+DSDKIVK LED E  LLRRHAD
Sbjct: 61  PNIIELFSNKPSDQDLLVRSLMQRKVTEVMHELIQNAEDSDKIVKVLEDNEGFLLRRHAD 120

Query: 121 GSAFVELLKKLDSRPQLALEVFNWRRRQEGSFPLTAEEYAKGITVAGRSKNVDLAIELFT 180
            SAFVELLK+L S+P LALEVFNWRRRQEGSFPLTAEEYAK IT+AGRSKNVDLA+ELFT
Sbjct: 121 SSAFVELLKQLGSQPHLALEVFNWRRRQEGSFPLTAEEYAKSITLAGRSKNVDLAVELFT 180

Query: 181 EASNRRIKTTSTYNALMGAYMFNGLVDKCHSLFRDLKREANCVPTIVTYNILISVFGRLM 240
           EASN+R+KTTS YNALMGAYM+NGL DKCHSLFRDLKR+ANCVPTIVTYNIL+SVFGRLM
Sbjct: 181 EASNKRVKTTSIYNALMGAYMYNGLADKCHSLFRDLKRDANCVPTIVTYNILLSVFGRLM 240

Query: 241 LVDHMEATLQEIRDLKLSPNVSTYNNLIAGYVTAWMWDRMEQTFLTMKASSVNPNTETFS 300
           LVDHMEATL+EI DL LSPNVSTYN+LIAGYVTAWMWD+MEQTFL MKASSV PNTET  
Sbjct: 241 LVDHMEATLREIHDLDLSPNVSTYNHLIAGYVTAWMWDKMEQTFLKMKASSVKPNTETLL 300

Query: 301 LMLRGYAHSGNLEKMEEMYELVRGHVSNKNFPLIRAMICAYCWSSIADRVKRIEALLKLI 360
           LMLRGYAHS NLEKMEEM+EL + HV +KNFPLIR MICAYC S+IAD+V +IEALLKLI
Sbjct: 301 LMLRGYAHSDNLEKMEEMHELSKDHVDDKNFPLIRTMICAYCRSTIADKVTKIEALLKLI 360

Query: 361 PEEEYRPWLNVLLIRVYAQEDWLEGMESSINEAFEHGTSVTTVHVMRSIIASYFRCNAVD 420
           P+EEYRPWLNVLLI+VYAQEDWLE ME+SINEAF+HGTSV T+HVMRSI ASYFRCNAVD
Sbjct: 361 PKEEYRPWLNVLLIKVYAQEDWLERMENSINEAFKHGTSVNTLHVMRSISASYFRCNAVD 420

Query: 421 KLTNFVKRAECSGWRICRSLYHCKMVMFASQKRLEEMECVLNEMKSINLDWTKKTFWILY 480
           KLTNFV RAECSGWRICRSLYHCKMVMFAS+KR EEME VL+EMK+INLDWTKKTFWILY
Sbjct: 421 KLTNFVSRAECSGWRICRSLYHCKMVMFASEKRFEEMESVLDEMKNINLDWTKKTFWILY 480

Query: 481 KAYSVSGCRYKVDQVVGLMCKLGYGIPLDSCPS 513
           KAYS+SGCRYKVDQV   MCKLGYGIP D  PS
Sbjct: 481 KAYSLSGCRYKVDQVSCYMCKLGYGIPSDLSPS 513

BLAST of MS020267 vs. NCBI nr
Match: XP_023007782.1 (pentatricopeptide repeat-containing protein At2g30780 [Cucurbita maxima] >XP_023007783.1 pentatricopeptide repeat-containing protein At2g30780 [Cucurbita maxima])

HSP 1 Score: 844.7 bits (2181), Expect = 4.1e-241
Identity = 420/509 (82.51%), Postives = 455/509 (89.39%), Query Frame = 0

Query: 1   MKQLWKISVPRRFLVKRNIFAPFTFPLTRTSNY-LILRGLHTQTHTSSLLPPSLSSFSLL 60
           MKQLWK SV RR LV RNIFAPF+F L   SNY +   GLHTQ+ +SS   PS+SSFSLL
Sbjct: 1   MKQLWKFSVRRRILVNRNIFAPFSFSLANNSNYDMFHHGLHTQSCSSSSPVPSISSFSLL 60

Query: 61  PNIIELFSNKPSDHDLLVRMNMQRKLTEVRHELIQNADDSDKIVKKLEDCEDLLLRRHAD 120
           PNIIELFSNKPSD DLLVR  MQRK+TEV HELIQNA+DSDKIVK LED E  LLRRHAD
Sbjct: 61  PNIIELFSNKPSDQDLLVRSIMQRKVTEVMHELIQNAEDSDKIVKVLEDNEGFLLRRHAD 120

Query: 121 GSAFVELLKKLDSRPQLALEVFNWRRRQEGSFPLTAEEYAKGITVAGRSKNVDLAIELFT 180
            S FVELLK+L S+P LALEVFNWRRRQEGSFPLTAEEYAK ITVAGRSKNVDLA+ELFT
Sbjct: 121 SSTFVELLKQLGSQPHLALEVFNWRRRQEGSFPLTAEEYAKSITVAGRSKNVDLAVELFT 180

Query: 181 EASNRRIKTTSTYNALMGAYMFNGLVDKCHSLFRDLKREANCVPTIVTYNILISVFGRLM 240
           +ASN+R+KTTS YNALMGAYM+NGL DKCHSLFRDLK +ANCVPTIVTYNIL+SVFGRLM
Sbjct: 181 KASNKRVKTTSIYNALMGAYMYNGLADKCHSLFRDLKTDANCVPTIVTYNILLSVFGRLM 240

Query: 241 LVDHMEATLQEIRDLKLSPNVSTYNNLIAGYVTAWMWDRMEQTFLTMKASSVNPNTETFS 300
           LVDHMEATL+EI DL LSPNVSTYN+LIAGYVTAWMWD+MEQTFL MKASSV PNT+TF 
Sbjct: 241 LVDHMEATLREIHDLDLSPNVSTYNHLIAGYVTAWMWDKMEQTFLKMKASSVKPNTDTFL 300

Query: 301 LMLRGYAHSGNLEKMEEMYELVRGHVSNKNFPLIRAMICAYCWSSIADRVKRIEALLKLI 360
           LMLRGYAHS NLEKMEEM+EL++ HV  KNFPLIR MICAYC S+IAD+V +IEALLKLI
Sbjct: 301 LMLRGYAHSDNLEKMEEMHELLKDHVDEKNFPLIRTMICAYCRSTIADKVTKIEALLKLI 360

Query: 361 PEEEYRPWLNVLLIRVYAQEDWLEGMESSINEAFEHGTSVTTVHVMRSIIASYFRCNAVD 420
           P+EEYRPWLNVLLI+VYAQEDWLE MESSINEAFEHGTSV T+HVMRSIIASY+RCNAVD
Sbjct: 361 PKEEYRPWLNVLLIKVYAQEDWLERMESSINEAFEHGTSVNTLHVMRSIIASYYRCNAVD 420

Query: 421 KLTNFVKRAECSGWRICRSLYHCKMVMFASQKRLEEMECVLNEMKSINLDWTKKTFWILY 480
           KLTNFV RAECSGWRICRSLYHCKMVMFAS+KR EEME VL+EMK++NLDWTKKTFWILY
Sbjct: 421 KLTNFVSRAECSGWRICRSLYHCKMVMFASEKRFEEMESVLDEMKNMNLDWTKKTFWILY 480

Query: 481 KAYSVSGCRYKVDQVVGLMCKLGYGIPLD 509
           KAYS+S CRYKVDQV   MCKLGYGIP D
Sbjct: 481 KAYSLSDCRYKVDQVACYMCKLGYGIPSD 509

BLAST of MS020267 vs. NCBI nr
Match: XP_023553032.1 (pentatricopeptide repeat-containing protein At2g30780 [Cucurbita pepo subsp. pepo] >XP_023553033.1 pentatricopeptide repeat-containing protein At2g30780 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 840.5 bits (2170), Expect = 7.7e-240
Identity = 422/513 (82.26%), Postives = 453/513 (88.30%), Query Frame = 0

Query: 1   MKQLWKISVPRRFLVKRNIFAPFTFPLTRTSNYLIL-RGLHTQTHTSSLLPPSLSSFSLL 60
           MKQLWK SV  RFLV RNIFAPF+F L   SN+ I   GLHTQ+ +SS   PS+SSFSLL
Sbjct: 1   MKQLWKFSVRHRFLVNRNIFAPFSFSLANNSNHFIFPHGLHTQSCSSSSPVPSISSFSLL 60

Query: 61  PNIIELFSNKPSDHDLLVRMNMQRKLTEVRHELIQNADDSDKIVKKLEDCEDLLLRRHAD 120
           PNIIELFSNKPSD DLLVR  MQRK+TEV HELIQNA+DSDKIVK LED E  LLRRHAD
Sbjct: 61  PNIIELFSNKPSDQDLLVRRLMQRKVTEVMHELIQNAEDSDKIVKVLEDNEGFLLRRHAD 120

Query: 121 GSAFVELLKKLDSRPQLALEVFNWRRRQEGSFPLTAEEYAKGITVAGRSKNVDLAIELFT 180
            SAFVELLK+L S+P LALEVFNWRRRQEGSFPLTAEEYAK ITVAGRSKNVDLA ELFT
Sbjct: 121 SSAFVELLKQLGSQPHLALEVFNWRRRQEGSFPLTAEEYAKSITVAGRSKNVDLAFELFT 180

Query: 181 EASNRRIKTTSTYNALMGAYMFNGLVDKCHSLFRDLKREANCVPTIVTYNILISVFGRLM 240
           EASN+R+K TSTYNALMGAYM+NGL DKCHSLFRDLK +ANCVPTIVTYNIL+SVFGRLM
Sbjct: 181 EASNKRVKRTSTYNALMGAYMYNGLADKCHSLFRDLKMDANCVPTIVTYNILLSVFGRLM 240

Query: 241 LVDHMEATLQEIRDLKLSPNVSTYNNLIAGYVTAWMWDRMEQTFLTMKASSVNPNTETFS 300
           LVDHMEATL+EI DL LSPNVSTYN+LIAGYVTAWMWD+MEQTFL MKASSV PNTET  
Sbjct: 241 LVDHMEATLREIHDLDLSPNVSTYNHLIAGYVTAWMWDKMEQTFLKMKASSVKPNTETLL 300

Query: 301 LMLRGYAHSGNLEKMEEMYELVRGHVSNKNFPLIRAMICAYCWSSIADRVKRIEALLKLI 360
           LMLRGYAHS NLEKMEEM+EL + HV + NFPLIR MICAYC S+IAD+V +IEALLKLI
Sbjct: 301 LMLRGYAHSDNLEKMEEMHELSKDHVDDMNFPLIRTMICAYCRSTIADKVTKIEALLKLI 360

Query: 361 PEEEYRPWLNVLLIRVYAQEDWLEGMESSINEAFEHGTSVTTVHVMRSIIASYFRCNAVD 420
           P+EEYRPWLNVLLI+VYAQED LE ME+SINEAFEHGTSV T+HVMRSI ASYFRCNAVD
Sbjct: 361 PKEEYRPWLNVLLIKVYAQEDLLERMENSINEAFEHGTSVNTLHVMRSISASYFRCNAVD 420

Query: 421 KLTNFVKRAECSGWRICRSLYHCKMVMFASQKRLEEMECVLNEMKSINLDWTKKTFWILY 480
           KLTNFV RAECSGWRICRSLYHCKMVMFAS+KR EEME VL+EMK+INLDWTKKTFWILY
Sbjct: 421 KLTNFVSRAECSGWRICRSLYHCKMVMFASEKRFEEMESVLDEMKNINLDWTKKTFWILY 480

Query: 481 KAYSVSGCRYKVDQVVGLMCKLGYGIPLDSCPS 513
           K YS+SGCRYKVDQV   MCKLGYGIP D  PS
Sbjct: 481 KTYSLSGCRYKVDQVSCYMCKLGYGIPSDLSPS 513

BLAST of MS020267 vs. ExPASy Swiss-Prot
Match: O49343 (Pentatricopeptide repeat-containing protein At2g30780 OS=Arabidopsis thaliana OX=3702 GN=At2g30780 PE=2 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 3.0e-130
Identity = 229/434 (52.76%), Postives = 310/434 (71.43%), Query Frame = 0

Query: 70  PSDHDLLVRMNMQRKLTEVRHELIQNADDSDKIVKKLEDCEDLLLRRHADGSAFVELLKK 129
           P+ H  LV     ++++ ++ EL+   +  +K    L+     L R + DG+  +EL+ +
Sbjct: 23  PAHHTDLV-----QRVSILKDELLTIGNSKEKFQNVLDQKGQWLFRTYRDGAGILELMDQ 82

Query: 130 LDSRPQLALEVFNWRRRQEG-SFPLTAEEYAKGITVAGRSKNVDLAIELFTEASNRRIKT 189
           L  R  LAL+V  WRR Q+    PLT+EEYAKGI +AGR+++++LA+ LF EA+ +R++T
Sbjct: 83  LFPRHYLALQVLEWRRGQKDYCIPLTSEEYAKGIKIAGRARDINLAVYLFDEAAKKRMQT 142

Query: 190 TSTYNALMGAYMFNGLVDKCHSLFRDLKREANCVPTIVTYNILISVFGRLMLVDHMEATL 249
            S YN+LM  YM+NGL ++C SLF+D +R+ +C PT+VTYNIL+SV+GRL++V +MEA  
Sbjct: 143 ASVYNSLMSVYMWNGLAEECQSLFKDFRRQTHCAPTVVTYNILVSVYGRLLMVKNMEAAF 202

Query: 250 QEIRDLKLSPNVSTYNNLIAGYVTAWMWDRMEQTFLTMKASSVNPNTETFSLMLRGYAHS 309
           +E++ +KL PN  TYN LIAGY+TAW WD+ME TF  MK   V P+T+T+ LMLRGYA+S
Sbjct: 203 EELQKVKLPPNSVTYNFLIAGYMTAWNWDKMEATFQEMKRGPVEPDTDTYQLMLRGYANS 262

Query: 310 GNLEKMEEMYELVRGHVSNKNFPLIRAMICAYCWSSIADRVKRIEALLKLIPEEEYRPWL 369
           GNL +MEEMYE+++  V   + PL+RAMICAYC  ++ DRV++IE LL L+  EEY PWL
Sbjct: 263 GNLNRMEEMYEVIKDQVGVNSGPLVRAMICAYCKKAVEDRVQKIENLLSLLSGEEYLPWL 322

Query: 370 NVLLIRVYAQEDWLEGMESSINEAFEHGTSVTTVHVMRSIIASYFRCNAVDKLTNFVKRA 429
           NVLLIR+YAQED++E MES INEAFE  T V    +MR+IIA+YFRCN VD L NFVKRA
Sbjct: 323 NVLLIRLYAQEDFVEAMESKINEAFEQKTCVNKSSIMRAIIAAYFRCNEVDNLANFVKRA 382

Query: 430 ECSGWRICRSLYHCKMVMFASQKRLEEMECVLNEMKSINLDWTKKTFWILYKAYSVSGCR 489
           E +GW++CRSLYHCK++M+ SQKR EEME V+NEM   N     KTF I+ KAY   G  
Sbjct: 383 ESAGWKLCRSLYHCKIMMYGSQKRFEEMEGVVNEMAETNYGLVTKTFAIMIKAYKNHGME 442

Query: 490 YKVDQVVGLMCKLG 503
              ++V G M K G
Sbjct: 443 SDAEKVKGKMLKRG 451

BLAST of MS020267 vs. ExPASy Swiss-Prot
Match: Q9ZU88 (Pentatricopeptide repeat-containing protein At2g48000 OS=Arabidopsis thaliana OX=3702 GN=At2g48000 PE=2 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 5.4e-111
Identity = 207/423 (48.94%), Postives = 293/423 (69.27%), Query Frame = 0

Query: 88  VRHELIQNADDSDKIVKKLEDCEDLLLRRHADGSAFVELLKKLDSRPQLALEVFNWRRRQ 147
           ++++L++  +DS K+   LE       R    G+AFVELL++L   P L+  VF+WRR +
Sbjct: 55  LKNQLLRFRNDSGKVASVLE-------RNKIQGAAFVELLRQLRPWPVLSQLVFDWRRNK 114

Query: 148 E--GSFPLTAEEYAKGITVAGRSKNVDLAIELFTEASNRRIKTTSTYNALMGAYMFNGLV 207
                 P+TA+EYAKGIT++GR KNVDLA+ LF E++N   KTTS YNALMGAY+ NGL 
Sbjct: 115 ALCDGLPMTADEYAKGITISGRLKNVDLALSLFHESAN---KTTSVYNALMGAYLCNGLS 174

Query: 208 DKCHSLFRDLKRE----ANCVPTIVTYNILISVFGRLMLVDHMEATLQEIRDLKLSPNVS 267
             C  LF D   +    ++  P++ TYNILIS++GRL++V+ ME+   +++ L + P+ S
Sbjct: 175 HHCEQLFLDFNSQQDGPSSSTPSVSTYNILISLYGRLIMVERMESVFLQLQQLNILPDSS 234

Query: 268 TYNNLIAGYVTAWMWDRMEQTFLTMKASSVNPNTETFSLMLRGYAHSGNLEKMEEMYELV 327
           TYNNLIAGY+ AW WD+ME TF +MK   V P   T+ LMLRGYA+SGNL +ME+MY+ V
Sbjct: 235 TYNNLIAGYIYAWDWDKMEATFHSMKNGLVKPTLATYLLMLRGYANSGNLLRMEDMYQAV 294

Query: 328 RGHVSNKNFPLIRAMICAYCWSSIADRVKRIEALLKLIPEEEYRPWLNVLLIRVYAQEDW 387
           + HV      LI +MICAY  S   DR+++I+ L KLIP++ Y+PWL +LL++VYA++D 
Sbjct: 295 KRHVDRNEIKLIESMICAYYRSCHKDRIRKIKTLSKLIPKKSYKPWLYLLLMQVYAKDDN 354

Query: 388 LEGMESSINEAFEHGTSVTTVHVMRSIIASYFRCNAVDKLTNFVKRAECSGWRICRSLYH 447
           L  ME+ I++A   G  + T  +MRSI+ASYFRCNAVDKL  FV+RA  +GW++ RS++H
Sbjct: 355 LHAMENFIDQAITKGLQIETDGIMRSIVASYFRCNAVDKLAKFVQRANSAGWKMSRSMFH 414

Query: 448 CKMVMFASQKRLEEMECVLNEMKSINLDWTKKTFWILYKAYSVS-GCRYKVDQVVGLMCK 504
             M+M+ SQKR +EME VL+EM+S  +  +KKT  IL + Y+ + G  +KV+QV G+M K
Sbjct: 415 GLMIMYGSQKRFKEMENVLSEMESFKISRSKKTLCILLRVYAATHGQEHKVNQVAGMMLK 467

BLAST of MS020267 vs. ExPASy Swiss-Prot
Match: Q9FKC3 (Pentatricopeptide repeat-containing protein At5g48730, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g48730 PE=2 SV=2)

HSP 1 Score: 122.5 bits (306), Expect = 1.4e-26
Identity = 99/375 (26.40%), Postives = 171/375 (45.60%), Query Frame = 0

Query: 133 RPQLALEVFNWRRRQEGSFPLTAEEYAKGITVAGRSKNVDLAIELFTEASNRR-IKTTST 192
           R + A++VF   R Q    P     Y K I + G+ K  + A ELF E  N   +     
Sbjct: 129 RWESAIQVFELLREQLWYKP-NVGIYVKLIVMLGKCKQPEKAHELFQEMINEGCVVNHEV 188

Query: 193 YNALMGAYMFNGLVDKCHSLFRDLKREANCVPTIVTYNILISVFGRLMLVDHMEATLQEI 252
           Y AL+ AY  +G  D   +L   +K   NC P + TY+ILI  F ++   D ++  L ++
Sbjct: 189 YTALVSAYSRSGRFDAAFTLLERMKSSHNCQPDVHTYSILIKSFLQVFAFDKVQDLLSDM 248

Query: 253 RDLKLSPNVSTYNNLIAGYVTAWMWDRMEQTFLTMKA-SSVNPNTETFSLMLRGYAHSGN 312
           R   + PN  TYN LI  Y  A M+  ME T + M       P++ T +  LR +  +G 
Sbjct: 249 RRQGIRPNTITYNTLIDAYGKAKMFVEMESTLIQMLGEDDCKPDSWTMNSTLRAFGGNGQ 308

Query: 313 LEKMEEMYELVRGHVSNKNFPLIRAMICAYCWSSIADRVKRIEALLKLIPEEEYRPWLNV 372
           +E ME  YE  +      N      ++ +Y  S      K++ A+++ + +  Y  W  V
Sbjct: 309 IEMMENCYEKFQSSGIEPNIRTFNILLDSYGKSG---NYKKMSAVMEYMQKYHY-SWTIV 368

Query: 373 ---LLIRVYAQEDWLEGMESSINEAFEHGTSVTTVHVMRSIIASYFRCNAVDKLTNFVKR 432
              ++I  + +   L+ ME  +    +      +   + S++ +Y R +  DK+   ++ 
Sbjct: 369 TYNVVIDAFGRAGDLKQME-YLFRLMQSERIFPSCVTLCSLVRAYGRASKADKIGGVLRF 428

Query: 433 AECSGWRICRSLYHCKMVMFASQKRLEEMECVLNEMKSINLDWTKKTFWILYKAYSVSGC 492
            E S  R+    ++C +  +   ++  EM+ VL  M+       K T+  + KAY +SG 
Sbjct: 429 IENSDIRLDLVFFNCLVDAYGRMEKFAEMKGVLELMEKKGFKPDKITYRTMVKAYRISGM 488

Query: 493 RYKVDQVVGLMCKLG 503
              V ++ G++  +G
Sbjct: 489 TTHVKELHGVVESVG 497

BLAST of MS020267 vs. ExPASy Swiss-Prot
Match: A7LN87 (Pentatricopeptide repeat-containing protein PPR5, chloroplastic OS=Zea mays OX=4577 GN=PPR5 PE=2 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 2.3e-21
Identity = 87/334 (26.05%), Postives = 160/334 (47.90%), Query Frame = 0

Query: 88  VRHELIQNADDSDKIVKKLEDCEDLLLRRHADGSAFVELLKKLDSRPQLALEVFNWRRRQ 147
           VR  L + A   +++V  L+    ++   H       E L + D+  Q  L+VF W ++Q
Sbjct: 61  VRSLLRRTAGGKERLVPVLDRHVRVVRTEHC--FLLFEELGRRDAWLQ-CLDVFRWMQKQ 120

Query: 148 EGSFPLTAEEYAKGITVAGRSKNVDLAIELFTEASNRRIK-TTSTYNALMGAYMFN---- 207
              +      Y+K I+V GR   + +A+ LF++  N   K  TS YN+L+GA++ +    
Sbjct: 121 RW-YVADNGIYSKLISVMGRKGQIRMAMWLFSQMRNSGCKPDTSVYNSLIGAHLHSRDKT 180

Query: 208 GLVDKCHSLFRDLKREANCVPTIVTYNILISVFGRLMLVDHMEATLQEIRDLKLSPNVST 267
             + K    F  +K    C PTIVTYNIL+  F +      ++   +++ +  +SP+V T
Sbjct: 181 KALAKALGYFEKMKCIERCQPTIVTYNILLRAFAQAGDTKQVDMLFKDLDESVVSPDVYT 240

Query: 268 YNNLIAGYVTAWMWDRMEQTFLTMKASSVNPNTETFSLMLRGYAHSGNLEKMEEMYELVR 327
           YN ++  Y    M   ME   + MK++   P+  TF++++  Y      +KME++++ + 
Sbjct: 241 YNGVLDAYGKNGMIKEMESVLVRMKSTQCRPDVITFNILIDSYGRKQTFDKMEQVFKSLL 300

Query: 328 GHVSNKNFPLIRAMICAYCWSSIADRVKRIEALLKLIPEEEYRP--WLNVLLIRVYAQED 387
                   P   +MI  Y  + + ++    E++++ + E  ++P       LI +YA  D
Sbjct: 301 RSKERPTHPTFNSMITNYGRARLREKA---ESVVEKMEELGFKPNYVTQECLIIMYAHCD 360

Query: 388 WLEGMESSINEAFEHGTSVTTVHV--MRSIIASY 413
            +       +E     TS T VH+  + S++ +Y
Sbjct: 361 CVSKARQVFDELV---TSQTKVHLSSLNSMLEAY 384

BLAST of MS020267 vs. ExPASy Swiss-Prot
Match: Q9SV96 (Pentatricopeptide repeat-containing protein At4g39620, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=EMB2453 PE=2 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 7.5e-20
Identity = 79/301 (26.25%), Postives = 135/301 (44.85%), Query Frame = 0

Query: 71  SDHDLLVRMNMQRKLTEVRHELIQNADDSDKIVKKLEDCEDLLLRRHADGSAFVELLKKL 130
           S   L  R + +R+   +   L+    D + +VK L+    ++   H       E L K 
Sbjct: 53  SRRKLAERESAERENRVLVRSLMSRISDREPLVKTLDKYVKVVRCDHC--FLLFEELGKS 112

Query: 131 DSRPQLALEVFNWRRRQEGSFPLTAEEYAKGITVAGRSKNVDLAIELFTEASNRRIK-TT 190
           D   Q  LEVF W ++Q    P     Y+K I+V G+     +A+ LF+E  N   +   
Sbjct: 113 DKWLQ-CLEVFRWMQKQRWYIPDNG-VYSKLISVMGKKGQTRMAMWLFSEMKNSGCRPDA 172

Query: 191 STYNALMGAYMFN----GLVDKCHSLFRDLKREANCVPTIVTYNILISVFGRLMLVDHME 250
           S YNAL+ A++        ++K       +K    C P +VTYNIL+  F +   VD + 
Sbjct: 173 SVYNALITAHLHTRDKAKALEKVRGYLDKMKGIERCQPNVVTYNILLRAFAQSGKVDQVN 232

Query: 251 ATLQEIRDLKLSPNVSTYNNLIAGYVTAWMWDRMEQTFLTMKASSVNPNTETFSLMLRGY 310
           A  +++    +SP+V T+N ++  Y    M   ME     M+++   P+  TF++++  Y
Sbjct: 233 ALFKDLDMSPVSPDVYTFNGVMDAYGKNGMIKEMEAVLTRMRSNECKPDIITFNVLIDSY 292

Query: 311 AHSGNLEKMEEMYELVRGHVSNKNFPLIRAMICAYCWSSIADRVKRIEALLKLIPEEEYR 367
                 EKME+ ++ +         P   +MI  Y  + + D+    E + K + +  Y 
Sbjct: 293 GKKQEFEKMEQTFKSLMRSKEKPTLPTFNSMIINYGKARMIDKA---EWVFKKMNDMNYI 346

BLAST of MS020267 vs. ExPASy TrEMBL
Match: A0A6J1CXA6 (pentatricopeptide repeat-containing protein At2g30780 OS=Momordica charantia OX=3673 GN=LOC111015285 PE=3 SV=1)

HSP 1 Score: 1028.1 bits (2657), Expect = 1.3e-296
Identity = 509/512 (99.41%), Postives = 510/512 (99.61%), Query Frame = 0

Query: 1   MKQLWKISVPRRFLVKRNIFAPFTFPLTRTSNYLILRGLHTQTHTSSLLPPSLSSFSLLP 60
           MKQLWKISVPRRFLVKRNIFAPFTFPLTRTSNYLILRGLHTQTHTSSLLPPSLSSFSLLP
Sbjct: 1   MKQLWKISVPRRFLVKRNIFAPFTFPLTRTSNYLILRGLHTQTHTSSLLPPSLSSFSLLP 60

Query: 61  NIIELFSNKPSDHDLLVRMNMQRKLTEVRHELIQNADDSDKIVKKLEDCEDLLLRRHADG 120
           NIIELFSNKPSDHDLLVRMNMQRKLTEVRHELIQNADDSDKIVKKLEDCEDLLLRRHADG
Sbjct: 61  NIIELFSNKPSDHDLLVRMNMQRKLTEVRHELIQNADDSDKIVKKLEDCEDLLLRRHADG 120

Query: 121 SAFVELLKKLDSRPQLALEVFNWRRRQEGSFPLTAEEYAKGITVAGRSKNVDLAIELFTE 180
           SAFVELLKKLDSRP LALEVFNWRRRQEGSFP+TAEEYAKGITVAGRSKNVDLAIELFTE
Sbjct: 121 SAFVELLKKLDSRPHLALEVFNWRRRQEGSFPMTAEEYAKGITVAGRSKNVDLAIELFTE 180

Query: 181 ASNRRIKTTSTYNALMGAYMFNGLVDKCHSLFRDLKREANCVPTIVTYNILISVFGRLML 240
           ASNRRIKTTSTYNALMGAYMFNGLVDKCHSLFRDLKREANCVPTIVTYNILISVFGRLML
Sbjct: 181 ASNRRIKTTSTYNALMGAYMFNGLVDKCHSLFRDLKREANCVPTIVTYNILISVFGRLML 240

Query: 241 VDHMEATLQEIRDLKLSPNVSTYNNLIAGYVTAWMWDRMEQTFLTMKASSVNPNTETFSL 300
           VDHMEATLQEIRDLKLSPNVSTYNNLIAGYVTAWMWDRMEQTFLTMKASSV PNTETFSL
Sbjct: 241 VDHMEATLQEIRDLKLSPNVSTYNNLIAGYVTAWMWDRMEQTFLTMKASSVKPNTETFSL 300

Query: 301 MLRGYAHSGNLEKMEEMYELVRGHVSNKNFPLIRAMICAYCWSSIADRVKRIEALLKLIP 360
           MLRGYAHSGNLEKMEEMYELVRGHVSNKNFPLIRAMICAYCWSSIADRVKRIEALLKLIP
Sbjct: 301 MLRGYAHSGNLEKMEEMYELVRGHVSNKNFPLIRAMICAYCWSSIADRVKRIEALLKLIP 360

Query: 361 EEEYRPWLNVLLIRVYAQEDWLEGMESSINEAFEHGTSVTTVHVMRSIIASYFRCNAVDK 420
           EEEYRPWLNVLLIRVYAQEDWLEGMESSINEAFEHGTSVTTVHVMRSIIASYFRCNAVDK
Sbjct: 361 EEEYRPWLNVLLIRVYAQEDWLEGMESSINEAFEHGTSVTTVHVMRSIIASYFRCNAVDK 420

Query: 421 LTNFVKRAECSGWRICRSLYHCKMVMFASQKRLEEMECVLNEMKSINLDWTKKTFWILYK 480
           LTNFVKRAECSGWRICRSLYHCKMVMFASQKRLEEMECVLNEMKSINLDWTKKTFWILYK
Sbjct: 421 LTNFVKRAECSGWRICRSLYHCKMVMFASQKRLEEMECVLNEMKSINLDWTKKTFWILYK 480

Query: 481 AYSVSGCRYKVDQVVGLMCKLGYGIPLDSCPS 513
           AYSVSGCRYKVDQVVGLMCKLGYGIPLDSCPS
Sbjct: 481 AYSVSGCRYKVDQVVGLMCKLGYGIPLDSCPS 512

BLAST of MS020267 vs. ExPASy TrEMBL
Match: A0A6J1E6A9 (pentatricopeptide repeat-containing protein At2g30780 OS=Cucurbita moschata OX=3662 GN=LOC111431181 PE=3 SV=1)

HSP 1 Score: 851.7 bits (2199), Expect = 1.6e-243
Identity = 425/513 (82.85%), Postives = 458/513 (89.28%), Query Frame = 0

Query: 1   MKQLWKISVPRRFLVKRNIFAPFTFPLTRTSNYLIL-RGLHTQTHTSSLLPPSLSSFSLL 60
           MKQLWK SV  RFLV RNIFAPF+F L   SN+ I   GLHTQ+ +SS   PS+SSFSLL
Sbjct: 1   MKQLWKFSVRHRFLVNRNIFAPFSFSLANNSNHFIFPHGLHTQSCSSSSPVPSISSFSLL 60

Query: 61  PNIIELFSNKPSDHDLLVRMNMQRKLTEVRHELIQNADDSDKIVKKLEDCEDLLLRRHAD 120
           PNIIELFSNKPSD DLLVR  MQRK+TEV HELIQNA+DSDKIVK LED E  LLRRHAD
Sbjct: 61  PNIIELFSNKPSDQDLLVRSIMQRKVTEVMHELIQNAEDSDKIVKVLEDNEGFLLRRHAD 120

Query: 121 GSAFVELLKKLDSRPQLALEVFNWRRRQEGSFPLTAEEYAKGITVAGRSKNVDLAIELFT 180
            SAFVELLK+L S+P LALEVFNWRRRQEGSFPLTAEEYAK IT+AGRSKNVDLA+ELFT
Sbjct: 121 SSAFVELLKQLGSQPHLALEVFNWRRRQEGSFPLTAEEYAKSITLAGRSKNVDLAVELFT 180

Query: 181 EASNRRIKTTSTYNALMGAYMFNGLVDKCHSLFRDLKREANCVPTIVTYNILISVFGRLM 240
           EASN+R+KTTS YNALMGAYM+NGL DKCHSLFRDLKR+ANCVPTIVTYNIL+SVFGRLM
Sbjct: 181 EASNKRVKTTSIYNALMGAYMYNGLADKCHSLFRDLKRDANCVPTIVTYNILLSVFGRLM 240

Query: 241 LVDHMEATLQEIRDLKLSPNVSTYNNLIAGYVTAWMWDRMEQTFLTMKASSVNPNTETFS 300
           LVDHMEATL+EI DL LSPNVSTYN+LIAGYVTAWMWD+MEQTFL MKASSV PNTET  
Sbjct: 241 LVDHMEATLREIHDLDLSPNVSTYNHLIAGYVTAWMWDKMEQTFLKMKASSVKPNTETLL 300

Query: 301 LMLRGYAHSGNLEKMEEMYELVRGHVSNKNFPLIRAMICAYCWSSIADRVKRIEALLKLI 360
           LMLRGYAHS NLEKMEEM+EL + HV +KNFPLIR MICAYC S+IAD+V +IEALLKLI
Sbjct: 301 LMLRGYAHSDNLEKMEEMHELSKDHVDDKNFPLIRTMICAYCRSTIADKVTKIEALLKLI 360

Query: 361 PEEEYRPWLNVLLIRVYAQEDWLEGMESSINEAFEHGTSVTTVHVMRSIIASYFRCNAVD 420
           P+EEYRPWLNVLLI+VYAQEDWLE ME+SINEAFEHGTSV T+HVMRSI ASYFRCNAVD
Sbjct: 361 PKEEYRPWLNVLLIKVYAQEDWLERMENSINEAFEHGTSVNTLHVMRSISASYFRCNAVD 420

Query: 421 KLTNFVKRAECSGWRICRSLYHCKMVMFASQKRLEEMECVLNEMKSINLDWTKKTFWILY 480
           KLTNFV RAECSGWRICRSLYHCKMVMFAS+KR EEME VL+EMK+INLDWTKKTFWILY
Sbjct: 421 KLTNFVSRAECSGWRICRSLYHCKMVMFASEKRFEEMESVLDEMKNINLDWTKKTFWILY 480

Query: 481 KAYSVSGCRYKVDQVVGLMCKLGYGIPLDSCPS 513
           KAYS+SGCRYKVDQV   MCKLGYGIP D  PS
Sbjct: 481 KAYSLSGCRYKVDQVSCYMCKLGYGIPSDLSPS 513

BLAST of MS020267 vs. ExPASy TrEMBL
Match: A0A6J1L8L9 (pentatricopeptide repeat-containing protein At2g30780 OS=Cucurbita maxima OX=3661 GN=LOC111500290 PE=3 SV=1)

HSP 1 Score: 844.7 bits (2181), Expect = 2.0e-241
Identity = 420/509 (82.51%), Postives = 455/509 (89.39%), Query Frame = 0

Query: 1   MKQLWKISVPRRFLVKRNIFAPFTFPLTRTSNY-LILRGLHTQTHTSSLLPPSLSSFSLL 60
           MKQLWK SV RR LV RNIFAPF+F L   SNY +   GLHTQ+ +SS   PS+SSFSLL
Sbjct: 1   MKQLWKFSVRRRILVNRNIFAPFSFSLANNSNYDMFHHGLHTQSCSSSSPVPSISSFSLL 60

Query: 61  PNIIELFSNKPSDHDLLVRMNMQRKLTEVRHELIQNADDSDKIVKKLEDCEDLLLRRHAD 120
           PNIIELFSNKPSD DLLVR  MQRK+TEV HELIQNA+DSDKIVK LED E  LLRRHAD
Sbjct: 61  PNIIELFSNKPSDQDLLVRSIMQRKVTEVMHELIQNAEDSDKIVKVLEDNEGFLLRRHAD 120

Query: 121 GSAFVELLKKLDSRPQLALEVFNWRRRQEGSFPLTAEEYAKGITVAGRSKNVDLAIELFT 180
            S FVELLK+L S+P LALEVFNWRRRQEGSFPLTAEEYAK ITVAGRSKNVDLA+ELFT
Sbjct: 121 SSTFVELLKQLGSQPHLALEVFNWRRRQEGSFPLTAEEYAKSITVAGRSKNVDLAVELFT 180

Query: 181 EASNRRIKTTSTYNALMGAYMFNGLVDKCHSLFRDLKREANCVPTIVTYNILISVFGRLM 240
           +ASN+R+KTTS YNALMGAYM+NGL DKCHSLFRDLK +ANCVPTIVTYNIL+SVFGRLM
Sbjct: 181 KASNKRVKTTSIYNALMGAYMYNGLADKCHSLFRDLKTDANCVPTIVTYNILLSVFGRLM 240

Query: 241 LVDHMEATLQEIRDLKLSPNVSTYNNLIAGYVTAWMWDRMEQTFLTMKASSVNPNTETFS 300
           LVDHMEATL+EI DL LSPNVSTYN+LIAGYVTAWMWD+MEQTFL MKASSV PNT+TF 
Sbjct: 241 LVDHMEATLREIHDLDLSPNVSTYNHLIAGYVTAWMWDKMEQTFLKMKASSVKPNTDTFL 300

Query: 301 LMLRGYAHSGNLEKMEEMYELVRGHVSNKNFPLIRAMICAYCWSSIADRVKRIEALLKLI 360
           LMLRGYAHS NLEKMEEM+EL++ HV  KNFPLIR MICAYC S+IAD+V +IEALLKLI
Sbjct: 301 LMLRGYAHSDNLEKMEEMHELLKDHVDEKNFPLIRTMICAYCRSTIADKVTKIEALLKLI 360

Query: 361 PEEEYRPWLNVLLIRVYAQEDWLEGMESSINEAFEHGTSVTTVHVMRSIIASYFRCNAVD 420
           P+EEYRPWLNVLLI+VYAQEDWLE MESSINEAFEHGTSV T+HVMRSIIASY+RCNAVD
Sbjct: 361 PKEEYRPWLNVLLIKVYAQEDWLERMESSINEAFEHGTSVNTLHVMRSIIASYYRCNAVD 420

Query: 421 KLTNFVKRAECSGWRICRSLYHCKMVMFASQKRLEEMECVLNEMKSINLDWTKKTFWILY 480
           KLTNFV RAECSGWRICRSLYHCKMVMFAS+KR EEME VL+EMK++NLDWTKKTFWILY
Sbjct: 421 KLTNFVSRAECSGWRICRSLYHCKMVMFASEKRFEEMESVLDEMKNMNLDWTKKTFWILY 480

Query: 481 KAYSVSGCRYKVDQVVGLMCKLGYGIPLD 509
           KAYS+S CRYKVDQV   MCKLGYGIP D
Sbjct: 481 KAYSLSDCRYKVDQVACYMCKLGYGIPSD 509

BLAST of MS020267 vs. ExPASy TrEMBL
Match: A0A0A0L4I3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G038710 PE=3 SV=1)

HSP 1 Score: 779.6 bits (2012), Expect = 7.8e-222
Identity = 386/514 (75.10%), Postives = 438/514 (85.21%), Query Frame = 0

Query: 1   MKQLWKISVPRRFLVKRNIFAPFTFPLTRTSNYLILRGLHTQTHTSSLLPPSLSSFSLLP 60
           MKQLW  SVPRR LV RNIFA F F LT T+   +  GLHTQ+ +SS  PPS+SSFSL+ 
Sbjct: 1   MKQLWMFSVPRRLLVSRNIFASFAFSLTNTNYCFLPHGLHTQSRSSSSPPPSISSFSLVA 60

Query: 61  NIIELFSNKPSDHDLLVRMNMQRKLTEVRHELIQNADDSDKIVKKLEDCEDLLLRRHADG 120
           N+IELFSNKP DH++L    M+RK+ EV HELI N++DS+KIVK LED +DLLL +H DG
Sbjct: 61  NLIELFSNKPDDHEILAICIMERKVLEVSHELILNSEDSNKIVKILEDSKDLLLWKHTDG 120

Query: 121 SAFVELLKKLDSRPQLALEVFNWRRRQEGSFPLTAEEYAKGITVAGRSKNVDLAIELFTE 180
           SAFVELLK+L S+P LALEVFNWRRRQ GSFPLT EEYAKGI VAG+SK++DLA+ LF E
Sbjct: 121 SAFVELLKQLGSQPNLALEVFNWRRRQGGSFPLTVEEYAKGIAVAGKSKHIDLAVGLFNE 180

Query: 181 ASNRRIKTTSTYNALMGAYMFNGLVDKCHSLFRDLKREANCVPTIVTYNILISVFGRLML 240
           ASN+R+K TSTYNALMG +MFNGL DKC+S+FRDLKR+A CVP IVTYNILISVFGRLML
Sbjct: 181 ASNKRVKATSTYNALMGVFMFNGLADKCNSVFRDLKRDAGCVPNIVTYNILISVFGRLML 240

Query: 241 VDHMEATLQEIRDLKLSPNVSTYNNLIAGYVTAWMWDRMEQTFLTMKASSVNPNTETFSL 300
           VDHMEAT++EI +L LSPNV+TYN+LIAGY+TAWMW RMEQ F+ MKASS+ PNTETF L
Sbjct: 241 VDHMEATMREIHNLNLSPNVNTYNSLIAGYITAWMWKRMEQAFMKMKASSITPNTETFLL 300

Query: 301 MLRGYAHSGNLEKMEEMYELVRGHVSNKNFPLIRAMICAYCWSSIADRVKRIEALLKLIP 360
           MLRGYAHS NLEKMEEM+  ++ HV+  NFPLIRAMI AY  SSI D+V +I+ALLKLIP
Sbjct: 301 MLRGYAHSDNLEKMEEMHHFLKDHVNKNNFPLIRAMIYAYSRSSITDKVHKIDALLKLIP 360

Query: 361 EEEYRPWLNVLLIRVYAQEDWLEGMESSINEAFEHGTSVTTVHVMRSIIASYFRCNAVDK 420
           EEEYRPWLNV LIRVYAQ D LE ME+SINEAFEHGTSV TVHVMRSIIASYFRCNAVDK
Sbjct: 361 EEEYRPWLNVKLIRVYAQADCLERMENSINEAFEHGTSVYTVHVMRSIIASYFRCNAVDK 420

Query: 421 LTNFVKRAECSGWRICRSLYHCKMVMFASQKRLEEMECVLNEMKSINLDWTKKTFWILYK 480
           L NF+ RAE SGWRICRSLYHCKMVMFASQ RLEEMECVL+EMK+ NLDW+KKTF+ILYK
Sbjct: 421 LINFISRAESSGWRICRSLYHCKMVMFASQNRLEEMECVLDEMKNFNLDWSKKTFYILYK 480

Query: 481 AYSVSGCRYKVDQVVGLMCKLGYGIPL--DSCPS 513
           AYS SGCRYK +QVV  MCKLGYG+P+  DS PS
Sbjct: 481 AYSTSGCRYKANQVVCRMCKLGYGVPVGWDSSPS 514

BLAST of MS020267 vs. ExPASy TrEMBL
Match: A0A5D3D7D2 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00940 PE=3 SV=1)

HSP 1 Score: 778.9 bits (2010), Expect = 1.3e-221
Identity = 386/514 (75.10%), Postives = 437/514 (85.02%), Query Frame = 0

Query: 1   MKQLWKISVPRRFLVKRNIFAPFTFPLTRTSNYLILRGLHTQTHTSSLLPPSLSSFSLLP 60
           MKQLW  SVPRR LV R IFAP  F L  T+   +  GLHTQ+ +SS  PPS+SSFSLL 
Sbjct: 135 MKQLWMFSVPRRLLVNRIIFAPSAFSLRNTNYCFLPHGLHTQSPSSSSPPPSISSFSLLT 194

Query: 61  NIIELFSNKPSDHDLLVRMNMQRKLTEVRHELIQNADDSDKIVKKLEDCEDLLLRRHADG 120
           N+IELFSNKPSDH +L R  MQ+K+ EV HELIQNA++SDKI+K LED EDLLLR+HAD 
Sbjct: 195 NLIELFSNKPSDHAILERSIMQKKVLEVSHELIQNAENSDKIMKILEDSEDLLLRKHADS 254

Query: 121 SAFVELLKKLDSRPQLALEVFNWRRRQEGSFPLTAEEYAKGITVAGRSKNVDLAIELFTE 180
            AFVELLK+L S+P LALEV NWRRRQ GSFPLTAEEYAKGI VAGRSK++DLA+ LFTE
Sbjct: 255 LAFVELLKQLGSQPYLALEVLNWRRRQGGSFPLTAEEYAKGIAVAGRSKHIDLAVGLFTE 314

Query: 181 ASNRRIKTTSTYNALMGAYMFNGLVDKCHSLFRDLKREANCVPTIVTYNILISVFGRLML 240
           ASN+R+KTTSTYNALMG YM+NGL DKC+SLFRDLKR+A+CVP IVTYNIL+S+FGRLML
Sbjct: 315 ASNKRVKTTSTYNALMGVYMYNGLADKCNSLFRDLKRDADCVPNIVTYNILLSLFGRLML 374

Query: 241 VDHMEATLQEIRDLKLSPNVSTYNNLIAGYVTAWMWDRMEQTFLTMKASSVNPNTETFSL 300
           VDHMEAT++EI DL LSPNV+TYN+L+AGY+TAWMW+RMEQTF+ MK+SS+ PNTETF L
Sbjct: 375 VDHMEATMREIHDLNLSPNVNTYNHLLAGYITAWMWNRMEQTFMKMKSSSIKPNTETFLL 434

Query: 301 MLRGYAHSGNLEKMEEMYELVRGHVSNKNFPLIRAMICAYCWSSIADRVKRIEALLKLIP 360
           MLRGYAHS NLEKMEEMY L++ HV+   FPLIR MI AYC SSI D+V +I+ALLKLIP
Sbjct: 435 MLRGYAHSDNLEKMEEMYHLLKDHVNENKFPLIRIMIYAYCRSSITDKVHKIDALLKLIP 494

Query: 361 EEEYRPWLNVLLIRVYAQEDWLEGMESSINEAFEHGTSVTTVHVMRSIIASYFRCNAVDK 420
           EEEYR WLN  LI +YAQ D LE ME SINEAFEHGTSV T  +MR IIASYFRCNAVDK
Sbjct: 495 EEEYRAWLNCKLISIYAQADCLERMEHSINEAFEHGTSVYTARLMRCIIASYFRCNAVDK 554

Query: 421 LTNFVKRAECSGWRICRSLYHCKMVMFASQKRLEEMECVLNEMKSINLDWTKKTFWILYK 480
           L NFV RAE +GWRICRSLYHCKMVMFASQ R EEMECVL+EMK+IN+DWTKKTF+ILYK
Sbjct: 555 LINFVSRAESAGWRICRSLYHCKMVMFASQNRFEEMECVLDEMKNINMDWTKKTFYILYK 614

Query: 481 AYSVSGCRYKVDQVVGLMCKLGYGIP--LDSCPS 513
           AYS SGCRYK +QVVG MCKLGYG+P  LDS PS
Sbjct: 615 AYSTSGCRYKANQVVGRMCKLGYGVPVDLDSSPS 648

BLAST of MS020267 vs. TAIR 10
Match: AT2G30780.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 466.8 bits (1200), Expect = 2.2e-131
Identity = 229/434 (52.76%), Postives = 310/434 (71.43%), Query Frame = 0

Query: 70  PSDHDLLVRMNMQRKLTEVRHELIQNADDSDKIVKKLEDCEDLLLRRHADGSAFVELLKK 129
           P+ H  LV     ++++ ++ EL+   +  +K    L+     L R + DG+  +EL+ +
Sbjct: 23  PAHHTDLV-----QRVSILKDELLTIGNSKEKFQNVLDQKGQWLFRTYRDGAGILELMDQ 82

Query: 130 LDSRPQLALEVFNWRRRQEG-SFPLTAEEYAKGITVAGRSKNVDLAIELFTEASNRRIKT 189
           L  R  LAL+V  WRR Q+    PLT+EEYAKGI +AGR+++++LA+ LF EA+ +R++T
Sbjct: 83  LFPRHYLALQVLEWRRGQKDYCIPLTSEEYAKGIKIAGRARDINLAVYLFDEAAKKRMQT 142

Query: 190 TSTYNALMGAYMFNGLVDKCHSLFRDLKREANCVPTIVTYNILISVFGRLMLVDHMEATL 249
            S YN+LM  YM+NGL ++C SLF+D +R+ +C PT+VTYNIL+SV+GRL++V +MEA  
Sbjct: 143 ASVYNSLMSVYMWNGLAEECQSLFKDFRRQTHCAPTVVTYNILVSVYGRLLMVKNMEAAF 202

Query: 250 QEIRDLKLSPNVSTYNNLIAGYVTAWMWDRMEQTFLTMKASSVNPNTETFSLMLRGYAHS 309
           +E++ +KL PN  TYN LIAGY+TAW WD+ME TF  MK   V P+T+T+ LMLRGYA+S
Sbjct: 203 EELQKVKLPPNSVTYNFLIAGYMTAWNWDKMEATFQEMKRGPVEPDTDTYQLMLRGYANS 262

Query: 310 GNLEKMEEMYELVRGHVSNKNFPLIRAMICAYCWSSIADRVKRIEALLKLIPEEEYRPWL 369
           GNL +MEEMYE+++  V   + PL+RAMICAYC  ++ DRV++IE LL L+  EEY PWL
Sbjct: 263 GNLNRMEEMYEVIKDQVGVNSGPLVRAMICAYCKKAVEDRVQKIENLLSLLSGEEYLPWL 322

Query: 370 NVLLIRVYAQEDWLEGMESSINEAFEHGTSVTTVHVMRSIIASYFRCNAVDKLTNFVKRA 429
           NVLLIR+YAQED++E MES INEAFE  T V    +MR+IIA+YFRCN VD L NFVKRA
Sbjct: 323 NVLLIRLYAQEDFVEAMESKINEAFEQKTCVNKSSIMRAIIAAYFRCNEVDNLANFVKRA 382

Query: 430 ECSGWRICRSLYHCKMVMFASQKRLEEMECVLNEMKSINLDWTKKTFWILYKAYSVSGCR 489
           E +GW++CRSLYHCK++M+ SQKR EEME V+NEM   N     KTF I+ KAY   G  
Sbjct: 383 ESAGWKLCRSLYHCKIMMYGSQKRFEEMEGVVNEMAETNYGLVTKTFAIMIKAYKNHGME 442

Query: 490 YKVDQVVGLMCKLG 503
              ++V G M K G
Sbjct: 443 SDAEKVKGKMLKRG 451

BLAST of MS020267 vs. TAIR 10
Match: AT2G48000.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 402.9 bits (1034), Expect = 3.8e-112
Identity = 207/423 (48.94%), Postives = 293/423 (69.27%), Query Frame = 0

Query: 88  VRHELIQNADDSDKIVKKLEDCEDLLLRRHADGSAFVELLKKLDSRPQLALEVFNWRRRQ 147
           ++++L++  +DS K+   LE       R    G+AFVELL++L   P L+  VF+WRR +
Sbjct: 55  LKNQLLRFRNDSGKVASVLE-------RNKIQGAAFVELLRQLRPWPVLSQLVFDWRRNK 114

Query: 148 E--GSFPLTAEEYAKGITVAGRSKNVDLAIELFTEASNRRIKTTSTYNALMGAYMFNGLV 207
                 P+TA+EYAKGIT++GR KNVDLA+ LF E++N   KTTS YNALMGAY+ NGL 
Sbjct: 115 ALCDGLPMTADEYAKGITISGRLKNVDLALSLFHESAN---KTTSVYNALMGAYLCNGLS 174

Query: 208 DKCHSLFRDLKRE----ANCVPTIVTYNILISVFGRLMLVDHMEATLQEIRDLKLSPNVS 267
             C  LF D   +    ++  P++ TYNILIS++GRL++V+ ME+   +++ L + P+ S
Sbjct: 175 HHCEQLFLDFNSQQDGPSSSTPSVSTYNILISLYGRLIMVERMESVFLQLQQLNILPDSS 234

Query: 268 TYNNLIAGYVTAWMWDRMEQTFLTMKASSVNPNTETFSLMLRGYAHSGNLEKMEEMYELV 327
           TYNNLIAGY+ AW WD+ME TF +MK   V P   T+ LMLRGYA+SGNL +ME+MY+ V
Sbjct: 235 TYNNLIAGYIYAWDWDKMEATFHSMKNGLVKPTLATYLLMLRGYANSGNLLRMEDMYQAV 294

Query: 328 RGHVSNKNFPLIRAMICAYCWSSIADRVKRIEALLKLIPEEEYRPWLNVLLIRVYAQEDW 387
           + HV      LI +MICAY  S   DR+++I+ L KLIP++ Y+PWL +LL++VYA++D 
Sbjct: 295 KRHVDRNEIKLIESMICAYYRSCHKDRIRKIKTLSKLIPKKSYKPWLYLLLMQVYAKDDN 354

Query: 388 LEGMESSINEAFEHGTSVTTVHVMRSIIASYFRCNAVDKLTNFVKRAECSGWRICRSLYH 447
           L  ME+ I++A   G  + T  +MRSI+ASYFRCNAVDKL  FV+RA  +GW++ RS++H
Sbjct: 355 LHAMENFIDQAITKGLQIETDGIMRSIVASYFRCNAVDKLAKFVQRANSAGWKMSRSMFH 414

Query: 448 CKMVMFASQKRLEEMECVLNEMKSINLDWTKKTFWILYKAYSVS-GCRYKVDQVVGLMCK 504
             M+M+ SQKR +EME VL+EM+S  +  +KKT  IL + Y+ + G  +KV+QV G+M K
Sbjct: 415 GLMIMYGSQKRFKEMENVLSEMESFKISRSKKTLCILLRVYAATHGQEHKVNQVAGMMLK 467

BLAST of MS020267 vs. TAIR 10
Match: AT5G48730.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 122.5 bits (306), Expect = 1.0e-27
Identity = 99/375 (26.40%), Postives = 171/375 (45.60%), Query Frame = 0

Query: 133 RPQLALEVFNWRRRQEGSFPLTAEEYAKGITVAGRSKNVDLAIELFTEASNRR-IKTTST 192
           R + A++VF   R Q    P     Y K I + G+ K  + A ELF E  N   +     
Sbjct: 129 RWESAIQVFELLREQLWYKP-NVGIYVKLIVMLGKCKQPEKAHELFQEMINEGCVVNHEV 188

Query: 193 YNALMGAYMFNGLVDKCHSLFRDLKREANCVPTIVTYNILISVFGRLMLVDHMEATLQEI 252
           Y AL+ AY  +G  D   +L   +K   NC P + TY+ILI  F ++   D ++  L ++
Sbjct: 189 YTALVSAYSRSGRFDAAFTLLERMKSSHNCQPDVHTYSILIKSFLQVFAFDKVQDLLSDM 248

Query: 253 RDLKLSPNVSTYNNLIAGYVTAWMWDRMEQTFLTMKA-SSVNPNTETFSLMLRGYAHSGN 312
           R   + PN  TYN LI  Y  A M+  ME T + M       P++ T +  LR +  +G 
Sbjct: 249 RRQGIRPNTITYNTLIDAYGKAKMFVEMESTLIQMLGEDDCKPDSWTMNSTLRAFGGNGQ 308

Query: 313 LEKMEEMYELVRGHVSNKNFPLIRAMICAYCWSSIADRVKRIEALLKLIPEEEYRPWLNV 372
           +E ME  YE  +      N      ++ +Y  S      K++ A+++ + +  Y  W  V
Sbjct: 309 IEMMENCYEKFQSSGIEPNIRTFNILLDSYGKSG---NYKKMSAVMEYMQKYHY-SWTIV 368

Query: 373 ---LLIRVYAQEDWLEGMESSINEAFEHGTSVTTVHVMRSIIASYFRCNAVDKLTNFVKR 432
              ++I  + +   L+ ME  +    +      +   + S++ +Y R +  DK+   ++ 
Sbjct: 369 TYNVVIDAFGRAGDLKQME-YLFRLMQSERIFPSCVTLCSLVRAYGRASKADKIGGVLRF 428

Query: 433 AECSGWRICRSLYHCKMVMFASQKRLEEMECVLNEMKSINLDWTKKTFWILYKAYSVSGC 492
            E S  R+    ++C +  +   ++  EM+ VL  M+       K T+  + KAY +SG 
Sbjct: 429 IENSDIRLDLVFFNCLVDAYGRMEKFAEMKGVLELMEKKGFKPDKITYRTMVKAYRISGM 488

Query: 493 RYKVDQVVGLMCKLG 503
              V ++ G++  +G
Sbjct: 489 TTHVKELHGVVESVG 497

BLAST of MS020267 vs. TAIR 10
Match: AT4G39620.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 100.1 bits (248), Expect = 5.3e-21
Identity = 79/301 (26.25%), Postives = 135/301 (44.85%), Query Frame = 0

Query: 71  SDHDLLVRMNMQRKLTEVRHELIQNADDSDKIVKKLEDCEDLLLRRHADGSAFVELLKKL 130
           S   L  R + +R+   +   L+    D + +VK L+    ++   H       E L K 
Sbjct: 53  SRRKLAERESAERENRVLVRSLMSRISDREPLVKTLDKYVKVVRCDHC--FLLFEELGKS 112

Query: 131 DSRPQLALEVFNWRRRQEGSFPLTAEEYAKGITVAGRSKNVDLAIELFTEASNRRIK-TT 190
           D   Q  LEVF W ++Q    P     Y+K I+V G+     +A+ LF+E  N   +   
Sbjct: 113 DKWLQ-CLEVFRWMQKQRWYIPDNG-VYSKLISVMGKKGQTRMAMWLFSEMKNSGCRPDA 172

Query: 191 STYNALMGAYMFN----GLVDKCHSLFRDLKREANCVPTIVTYNILISVFGRLMLVDHME 250
           S YNAL+ A++        ++K       +K    C P +VTYNIL+  F +   VD + 
Sbjct: 173 SVYNALITAHLHTRDKAKALEKVRGYLDKMKGIERCQPNVVTYNILLRAFAQSGKVDQVN 232

Query: 251 ATLQEIRDLKLSPNVSTYNNLIAGYVTAWMWDRMEQTFLTMKASSVNPNTETFSLMLRGY 310
           A  +++    +SP+V T+N ++  Y    M   ME     M+++   P+  TF++++  Y
Sbjct: 233 ALFKDLDMSPVSPDVYTFNGVMDAYGKNGMIKEMEAVLTRMRSNECKPDIITFNVLIDSY 292

Query: 311 AHSGNLEKMEEMYELVRGHVSNKNFPLIRAMICAYCWSSIADRVKRIEALLKLIPEEEYR 367
                 EKME+ ++ +         P   +MI  Y  + + D+    E + K + +  Y 
Sbjct: 293 GKKQEFEKMEQTFKSLMRSKEKPTLPTFNSMIINYGKARMIDKA---EWVFKKMNDMNYI 346

BLAST of MS020267 vs. TAIR 10
Match: AT4G39620.2 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 100.1 bits (248), Expect = 5.3e-21
Identity = 79/301 (26.25%), Postives = 135/301 (44.85%), Query Frame = 0

Query: 71  SDHDLLVRMNMQRKLTEVRHELIQNADDSDKIVKKLEDCEDLLLRRHADGSAFVELLKKL 130
           S   L  R + +R+   +   L+    D + +VK L+    ++   H       E L K 
Sbjct: 53  SRRKLAERESAERENRVLVRSLMSRISDREPLVKTLDKYVKVVRCDHC--FLLFEELGKS 112

Query: 131 DSRPQLALEVFNWRRRQEGSFPLTAEEYAKGITVAGRSKNVDLAIELFTEASNRRIK-TT 190
           D   Q  LEVF W ++Q    P     Y+K I+V G+     +A+ LF+E  N   +   
Sbjct: 113 DKWLQ-CLEVFRWMQKQRWYIPDNG-VYSKLISVMGKKGQTRMAMWLFSEMKNSGCRPDA 172

Query: 191 STYNALMGAYMFN----GLVDKCHSLFRDLKREANCVPTIVTYNILISVFGRLMLVDHME 250
           S YNAL+ A++        ++K       +K    C P +VTYNIL+  F +   VD + 
Sbjct: 173 SVYNALITAHLHTRDKAKALEKVRGYLDKMKGIERCQPNVVTYNILLRAFAQSGKVDQVN 232

Query: 251 ATLQEIRDLKLSPNVSTYNNLIAGYVTAWMWDRMEQTFLTMKASSVNPNTETFSLMLRGY 310
           A  +++    +SP+V T+N ++  Y    M   ME     M+++   P+  TF++++  Y
Sbjct: 233 ALFKDLDMSPVSPDVYTFNGVMDAYGKNGMIKEMEAVLTRMRSNECKPDIITFNVLIDSY 292

Query: 311 AHSGNLEKMEEMYELVRGHVSNKNFPLIRAMICAYCWSSIADRVKRIEALLKLIPEEEYR 367
                 EKME+ ++ +         P   +MI  Y  + + D+    E + K + +  Y 
Sbjct: 293 GKKQEFEKMEQTFKSLMRSKEKPTLPTFNSMIINYGKARMIDKA---EWVFKKMNDMNYI 346

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022145941.12.6e-29699.41pentatricopeptide repeat-containing protein At2g30780 [Momordica charantia] >XP_... [more]
XP_022923507.13.4e-24382.85pentatricopeptide repeat-containing protein At2g30780 [Cucurbita moschata] >XP_0... [more]
KAG7015626.12.8e-24282.65Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma sub... [more]
XP_023007782.14.1e-24182.51pentatricopeptide repeat-containing protein At2g30780 [Cucurbita maxima] >XP_023... [more]
XP_023553032.17.7e-24082.26pentatricopeptide repeat-containing protein At2g30780 [Cucurbita pepo subsp. pep... [more]
Match NameE-valueIdentityDescription
O493433.0e-13052.76Pentatricopeptide repeat-containing protein At2g30780 OS=Arabidopsis thaliana OX... [more]
Q9ZU885.4e-11148.94Pentatricopeptide repeat-containing protein At2g48000 OS=Arabidopsis thaliana OX... [more]
Q9FKC31.4e-2626.40Pentatricopeptide repeat-containing protein At5g48730, chloroplastic OS=Arabidop... [more]
A7LN872.3e-2126.05Pentatricopeptide repeat-containing protein PPR5, chloroplastic OS=Zea mays OX=4... [more]
Q9SV967.5e-2026.25Pentatricopeptide repeat-containing protein At4g39620, chloroplastic OS=Arabidop... [more]
Match NameE-valueIdentityDescription
A0A6J1CXA61.3e-29699.41pentatricopeptide repeat-containing protein At2g30780 OS=Momordica charantia OX=... [more]
A0A6J1E6A91.6e-24382.85pentatricopeptide repeat-containing protein At2g30780 OS=Cucurbita moschata OX=3... [more]
A0A6J1L8L92.0e-24182.51pentatricopeptide repeat-containing protein At2g30780 OS=Cucurbita maxima OX=366... [more]
A0A0A0L4I37.8e-22275.10Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G038710 PE=3 SV=1[more]
A0A5D3D7D21.3e-22175.10Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
Match NameE-valueIdentityDescription
AT2G30780.12.2e-13152.76Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT2G48000.13.8e-11248.94Pentatricopeptide repeat (PPR) superfamily protein [more]
AT5G48730.11.0e-2726.40Pentatricopeptide repeat (PPR) superfamily protein [more]
AT4G39620.15.3e-2126.25Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT4G39620.25.3e-2126.25Tetratricopeptide repeat (TPR)-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 188..235
e-value: 1.3E-10
score: 41.3
coord: 258..306
e-value: 6.6E-11
score: 42.3
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 191..224
e-value: 9.5E-5
score: 20.3
coord: 226..260
e-value: 1.1E-4
score: 20.2
coord: 297..320
e-value: 6.9E-4
score: 17.6
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 294..324
score: 8.53891
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 259..293
score: 9.722731
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 224..258
score: 8.560833
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 188..223
score: 8.801982
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 368..510
e-value: 1.3E-7
score: 33.1
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 253..322
e-value: 5.1E-14
score: 54.2
coord: 84..252
e-value: 2.0E-25
score: 91.4
IPR044179Pentatricopeptide repeat-containing protein PPR5-likePANTHERPTHR47874EXPRESSED PROTEINcoord: 27..511
NoneNo IPR availablePANTHERPTHR47874:SF1OS05G0407900 PROTEINcoord: 27..511

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS020267.1MS020267.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003729 mRNA binding
molecular_function GO:0005515 protein binding