MS020239 (gene) Bitter gourd (TR) v1

BLAST of MS020239 vs. NCBI nr
Match: XP_022156705.1 (amidase 1 [Momordica charantia] >XP_022156710.1 amidase 1 [Momordica charantia])

HSP 1 Score: 834.7 bits (2155), Expect = 3.7e-238
Identity = 425/452 (94.03%), Postives = 425/452 (94.03%), Query Frame = 0

Query: 1   MAVQDHGAFVEKFLLQPSSPSDELPLAGLTFAVKDIFDMDGYVTGFGNPEWARTHPPANQ 60
           MAVQDHGAFVEKFLLQPSSPSDELPLAGLTFAVKDIFDMDGYVTGFGNPEWARTHPPANQ
Sbjct: 1   MAVQDHGAFVEKFLLQPSSPSDELPLAGLTFAVKDIFDMDGYVTGFGNPEWARTHPPANQ 60

Query: 61  TAPTVSTILRGGATCIGRTVMDEMAYSINGENIHYGTPQNPYALDRIPGGSSSGSAVAVG 120
           TAPTVSTILRGGATCIGRTVMDEMAYSINGENIHYGTPQNPYALDRIPGGSSSGSAVAVG
Sbjct: 61  TAPTVSTILRGGATCIGRTVMDEMAYSINGENIHYGTPQNPYALDRIPGGSSSGSAVAVG 120

Query: 121 AKLVDFSLGKFLTVPCFVGTDTGGSIRVPASYCGILGFRPSHGAVSTSGVIPMSQSFDTV 180
           AKLVDFSL          GTDTGGSIRVPASYCGILGFRPSHGAVSTSGVIPMSQSFDTV
Sbjct: 121 AKLVDFSL----------GTDTGGSIRVPASYCGILGFRPSHGAVSTSGVIPMSQSFDTV 180

Query: 181 GWFARDLAVFKRVGRLLLQQSVVELIRPTQVLIAEDCFKLSSIPSKRLTQAFVNSVKKLF 240
           GWFARDLAVFKRVGRLLLQQS VELIRPTQVLIAEDCFKLSSIPSKRLTQAFVNSVKKLF
Sbjct: 181 GWFARDLAVFKRVGRLLLQQSEVELIRPTQVLIAEDCFKLSSIPSKRLTQAFVNSVKKLF 240

Query: 241 GGHLIKEISLGNYVDERVPSLKHFMIEENAAHKHSIPSLAALARSMQLLQRYEFKTNHEE 300
           GGHLIKEISLGNYVDERVPSLKHFMIEENAAHKHSIPSLAALARSMQLLQRYEFKTNHEE
Sbjct: 241 GGHLIKEISLGNYVDERVPSLKHFMIEENAAHKHSIPSLAALARSMQLLQRYEFKTNHEE 300

Query: 301 WIRTCNPHFGPGISERISDAMKTTDENTDLSRSIKTELREALAALLEDFGVLAIPTVPGP 360
           WIRTCNPHFGPGISERISDAMKTTDENTDLSRSIKTELREALAALLEDFGVLAIPTVPGP
Sbjct: 301 WIRTCNPHFGPGISERISDAMKTTDENTDLSRSIKTELREALAALLEDFGVLAIPTVPGP 360

Query: 361 PPKLNTNISELHDFRSKAFSLLSIAGVSGFCQALSLSLPPFFSLSLLKVNIPLGLYNGLP 420
           PPKLNTNISELHDFRSKAFSLLSIAGVSGFCQ                VNIPLGLYNGLP
Sbjct: 361 PPKLNTNISELHDFRSKAFSLLSIAGVSGFCQ----------------VNIPLGLYNGLP 420

Query: 421 VSISLLAKHGADGFLLNVVETIYNTLKEEVSC 453
           VSISLLAKHGADGFLLNVVETIYNTLKEEVSC
Sbjct: 421 VSISLLAKHGADGFLLNVVETIYNTLKEEVSC 426

BLAST of MS020239 vs. NCBI nr
Match: XP_022968725.1 (amidase 1 isoform X3 [Cucurbita maxima])

HSP 1 Score: 731.1 bits (1886), Expect = 5.8e-207
Identity = 360/450 (80.00%), Postives = 398/450 (88.44%), Query Frame = 0

Query: 1   MAVQDHGAFVEKFLLQPSSPSDELPLAGLTFAVKDIFDMDGYVTGFGNPEWARTHPPANQ 60
           MAVQDHGAF+EKFLLQPSSPSD+LPLAGLTFAVKDIFD+DGYVTGFGNPEW RTHPPANQ
Sbjct: 1   MAVQDHGAFMEKFLLQPSSPSDQLPLAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPANQ 60

Query: 61  TAPTVSTILRGGATCIGRTVMDEMAYSINGENIHYGTPQNPYALDRIPGGSSSGSAVAVG 120
           TAP VS+IL+GGATCIG+T+MDEMAYSINGENIHYGTPQNP+A DRIPGGSSSGSAVAVG
Sbjct: 61  TAPAVSSILKGGATCIGKTIMDEMAYSINGENIHYGTPQNPHASDRIPGGSSSGSAVAVG 120

Query: 121 AKLVDFSLGKFLTVPCFVGTDTGGSIRVPASYCGILGFRPSHGAVSTSGVIPMSQSFDTV 180
           AKLVDFSL          GTDTGGS+RVPASYCGI GFRPSHGAVSTSGVIPM+QSFDTV
Sbjct: 121 AKLVDFSL----------GTDTGGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTV 180

Query: 181 GWFARDLAVFKRVGRLLLQQSVVELIRPTQVLIAEDCFKLSSIPSKRLTQAFVNSVKKLF 240
           GWFARD  V KRVG+LLLQQ   E  +PTQVLIAEDC KLS IPS+RLTQ FVN+V KLF
Sbjct: 181 GWFARDPIVLKRVGQLLLQQPEFEHHKPTQVLIAEDCMKLSCIPSERLTQVFVNAVTKLF 240

Query: 241 GGHLIKEISLGNYVDERVPSLKHFMIEENAAHKHSIPSLAALARSMQLLQRYEFKTNHEE 300
           GGHLIK++SLGNYV+++VPSLKHFMIE NA H+HSIPSLAALARSMQLLQRYEFK NHEE
Sbjct: 241 GGHLIKQVSLGNYVEDKVPSLKHFMIEGNAGHEHSIPSLAALARSMQLLQRYEFKINHEE 300

Query: 301 WIRTCNPHFGPGISERISDAMKTTDENTDLSRSIKTELREALAALLEDFGVLAIPTVPGP 360
           W+RTCNPH GPGISER+ +AM+TTDEN DL RSIK +LREALA+LLEDFGVL IPTVPGP
Sbjct: 301 WVRTCNPHLGPGISERVLEAMRTTDENIDLCRSIKVKLREALASLLEDFGVLVIPTVPGP 360

Query: 361 PPKLNTNISELHDFRSKAFSLLSIAGVSGFCQALSLSLPPFFSLSLLKVNIPLGLYNGLP 420
           PP+LNT++S+LHDFR+KAFSLLSIAGVSG CQ                V+IPLGLYNGLP
Sbjct: 361 PPELNTDVSKLHDFRAKAFSLLSIAGVSGLCQ----------------VSIPLGLYNGLP 420

Query: 421 VSISLLAKHGADGFLLNVVETIYNTLKEEV 451
           VS+SLLAKHG+DGFLLNVV+++YNTLK+EV
Sbjct: 421 VSLSLLAKHGSDGFLLNVVDSLYNTLKQEV 424

BLAST of MS020239 vs. NCBI nr
Match: TYK10232.1 (amidase 1-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 729.2 bits (1881), Expect = 2.2e-206
Identity = 365/450 (81.11%), Postives = 398/450 (88.44%), Query Frame = 0

Query: 1   MAVQDHGAFVEKFLLQPSSPSDELPLAGLTFAVKDIFDMDGYVTGFGNPEWARTHPPANQ 60
           MAVQ +GAF+EKFLLQPSSPSD+LPL GLTFAVKDIFDMDG+VTGFGNPEW RTHPPAN 
Sbjct: 46  MAVQ-YGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 105

Query: 61  TAPTVSTILRGGATCIGRTVMDEMAYSINGENIHYGTPQNPYALDRIPGGSSSGSAVAVG 120
           TAPTVSTILRGGATCIGRT+MDEMAYSINGEN HYGTPQNP A DR+PGGSSSGSAVAVG
Sbjct: 106 TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 165

Query: 121 AKLVDFSLGKFLTVPCFVGTDTGGSIRVPASYCGILGFRPSHGAVSTSGVIPMSQSFDTV 180
           A LVDFSLG       FVGTDTGGS+RVPASYCGILGFRPSHGAVSTSGV+PM+QSFDTV
Sbjct: 166 ANLVDFSLG------TFVGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTV 225

Query: 181 GWFARDLAVFKRVGRLLLQQSVVELIRPTQVLIAEDCFKLSSIPSKRLTQAFVNSVKKLF 240
           GWFARD  VFK+VG LLLQQ  VE  +PTQVLIAEDCFKLSSIPS+RLTQAFVNSVKKLF
Sbjct: 226 GWFARDPVVFKKVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLF 285

Query: 241 GGHLIKEISLGNYVDERVPSLKHFMIEENAAHKHSIPSLAALARSMQLLQRYEFKTNHEE 300
           GGHLIK+++LGNYV+E+VPSLK FMIE NA ++HSIPSLAALARS+QLLQRYEFK NHEE
Sbjct: 286 GGHLIKQVNLGNYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEE 345

Query: 301 WIRTCNPHFGPGISERISDAMKTTDENTDLSRSIKTELREALAALLEDFGVLAIPTVPGP 360
           W+RT NPH GPGISER+S+AM+ TDEN DL  SIK ELR ALAALLEDFGVLAIPTVPGP
Sbjct: 346 WVRTYNPHLGPGISERVSEAMRATDENIDLCHSIKIELRRALAALLEDFGVLAIPTVPGP 405

Query: 361 PPKLNTNISELHDFRSKAFSLLSIAGVSGFCQALSLSLPPFFSLSLLKVNIPLGLYNGLP 420
           PPKLNT++SELHDFR+KAFSLLSIAGVSGFCQ                V+IPLGLYNGLP
Sbjct: 406 PPKLNTDVSELHDFRAKAFSLLSIAGVSGFCQ----------------VSIPLGLYNGLP 465

Query: 421 VSISLLAKHGADGFLLNVVETIYNTLKEEV 451
           VSISL+A HG+DGFLLNVV ++YNTL+EEV
Sbjct: 466 VSISLVANHGSDGFLLNVVHSLYNTLEEEV 472

BLAST of MS020239 vs. NCBI nr
Match: XP_022968726.1 (amidase 1 isoform X4 [Cucurbita maxima])

HSP 1 Score: 726.1 bits (1873), Expect = 1.9e-205
Identity = 358/450 (79.56%), Postives = 396/450 (88.00%), Query Frame = 0

Query: 1   MAVQDHGAFVEKFLLQPSSPSDELPLAGLTFAVKDIFDMDGYVTGFGNPEWARTHPPANQ 60
           MAVQDHGAF+EKF+LQ SSPSD LPLAGLTFAVKDIFD+DGYVTGFGNPEW RTHPPANQ
Sbjct: 1   MAVQDHGAFIEKFILQLSSPSDPLPLAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPANQ 60

Query: 61  TAPTVSTILRGGATCIGRTVMDEMAYSINGENIHYGTPQNPYALDRIPGGSSSGSAVAVG 120
           TAP VS+IL+GGATCIG+T+MDEMAYSINGENIHYGTPQNP+A DRIPGGSSSGSAVAVG
Sbjct: 61  TAPAVSSILKGGATCIGKTIMDEMAYSINGENIHYGTPQNPHASDRIPGGSSSGSAVAVG 120

Query: 121 AKLVDFSLGKFLTVPCFVGTDTGGSIRVPASYCGILGFRPSHGAVSTSGVIPMSQSFDTV 180
           AKLVDFSL          GTDTGGS+RVPASYCGI GFRPSHGAVSTSGVIPM+QSFDTV
Sbjct: 121 AKLVDFSL----------GTDTGGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTV 180

Query: 181 GWFARDLAVFKRVGRLLLQQSVVELIRPTQVLIAEDCFKLSSIPSKRLTQAFVNSVKKLF 240
           GWFARD  V KRVG+LLLQQ   E  +PTQVLIAEDC KLS IPS+RLTQ FVN+V KLF
Sbjct: 181 GWFARDPIVLKRVGQLLLQQPEFEHHKPTQVLIAEDCMKLSCIPSERLTQVFVNAVTKLF 240

Query: 241 GGHLIKEISLGNYVDERVPSLKHFMIEENAAHKHSIPSLAALARSMQLLQRYEFKTNHEE 300
           GGHLIK++SLGNYV+++VPSLKHFMIE NA H+HSIPSLAALARSMQLLQRYEFK NHEE
Sbjct: 241 GGHLIKQVSLGNYVEDKVPSLKHFMIEGNAGHEHSIPSLAALARSMQLLQRYEFKINHEE 300

Query: 301 WIRTCNPHFGPGISERISDAMKTTDENTDLSRSIKTELREALAALLEDFGVLAIPTVPGP 360
           W+RTCNPH GPGISER+ +AM+TTDEN DL RSIK +LREALA+LLEDFGVL IPTVPGP
Sbjct: 301 WVRTCNPHLGPGISERVLEAMRTTDENIDLCRSIKVKLREALASLLEDFGVLVIPTVPGP 360

Query: 361 PPKLNTNISELHDFRSKAFSLLSIAGVSGFCQALSLSLPPFFSLSLLKVNIPLGLYNGLP 420
           PP+LNT++S+LHDFR+KAFSLLSIAGVSG CQ                V+IPLGLYNGLP
Sbjct: 361 PPELNTDVSKLHDFRAKAFSLLSIAGVSGLCQ----------------VSIPLGLYNGLP 420

Query: 421 VSISLLAKHGADGFLLNVVETIYNTLKEEV 451
           VS+SLLAKHG+DGFLLNVV+++YNTLK+EV
Sbjct: 421 VSLSLLAKHGSDGFLLNVVDSLYNTLKQEV 424

BLAST of MS020239 vs. NCBI nr
Match: KAA0050880.1 (amidase 1-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 726.1 bits (1873), Expect = 1.9e-205
Identity = 364/450 (80.89%), Postives = 397/450 (88.22%), Query Frame = 0

Query: 1   MAVQDHGAFVEKFLLQPSSPSDELPLAGLTFAVKDIFDMDGYVTGFGNPEWARTHPPANQ 60
           MAVQ +GAF+EKFLLQPSSPSD+LPL GLTFAVKDIFDMDG+VTGFGNPEW RTHPPAN 
Sbjct: 46  MAVQ-YGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 105

Query: 61  TAPTVSTILRGGATCIGRTVMDEMAYSINGENIHYGTPQNPYALDRIPGGSSSGSAVAVG 120
           TAPTVSTILRGGATCIGRT+MDEMAYSINGEN HYGTPQNP A DR+PGGSSSGSAVAVG
Sbjct: 106 TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 165

Query: 121 AKLVDFSLGKFLTVPCFVGTDTGGSIRVPASYCGILGFRPSHGAVSTSGVIPMSQSFDTV 180
           A LVDFSLG       FVGTDTGGS+RVPASYCGILGFRPSHGAVSTSGV+PM+QSFDTV
Sbjct: 166 ANLVDFSLG------TFVGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTV 225

Query: 181 GWFARDLAVFKRVGRLLLQQSVVELIRPTQVLIAEDCFKLSSIPSKRLTQAFVNSVKKLF 240
           GWFARD  VFK+VG LLLQQ  VE  +PTQVLIAEDCFKLSSIPS+RLTQAFVNSVKKLF
Sbjct: 226 GWFARDPVVFKKVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLF 285

Query: 241 GGHLIKEISLGNYVDERVPSLKHFMIEENAAHKHSIPSLAALARSMQLLQRYEFKTNHEE 300
           GGHLIK+++LGNYV+E+VPSLK FMIE NA ++HSIPSLAALARS+QLLQRYEFK NHEE
Sbjct: 286 GGHLIKQVNLGNYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEE 345

Query: 301 WIRTCNPHFGPGISERISDAMKTTDENTDLSRSIKTELREALAALLEDFGVLAIPTVPGP 360
           W+RT NPH GPGISER+S+AM+ TDEN DL  SIK ELR ALAALLEDFGVLAIPTVPGP
Sbjct: 346 WVRTYNPHLGPGISERVSEAMRATDENIDLCHSIKIELRRALAALLEDFGVLAIPTVPGP 405

Query: 361 PPKLNTNISELHDFRSKAFSLLSIAGVSGFCQALSLSLPPFFSLSLLKVNIPLGLYNGLP 420
           PPKLNT++SELHDFR+KAFSLLSIAGVSGFCQ                V+IPL LYNGLP
Sbjct: 406 PPKLNTDVSELHDFRAKAFSLLSIAGVSGFCQ----------------VSIPLRLYNGLP 465

Query: 421 VSISLLAKHGADGFLLNVVETIYNTLKEEV 451
           VSISL+A HG+DGFLLNVV ++YNTL+EEV
Sbjct: 466 VSISLVANHGSDGFLLNVVHSLYNTLEEEV 472

BLAST of MS020239 vs. ExPASy Swiss-Prot
Match: Q9FR37 (Amidase 1 OS=Arabidopsis thaliana OX=3702 GN=AMI1 PE=1 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 3.7e-148
Identity = 266/442 (60.18%), Postives = 329/442 (74.43%), Query Frame = 0

Query: 5   DHGAFVEKFLLQPSSPSDELP-LAGLTFAVKDIFDMDGYVTGFGNPEWARTHPPANQTAP 64
           D GAF+EK  + P+S S   P L GLTFA+KDIFD++G VTGFGNP+W RTH  A  TAP
Sbjct: 6   DFGAFIEKVTISPTSTSSSPPSLQGLTFAIKDIFDVEGRVTGFGNPDWLRTHSAATSTAP 65

Query: 65  TVSTILRGGATCIGRTVMDEMAYSINGENIHYGTPQNPYALDRIPGGSSSGSAVAVGAKL 124
            VS++L  GAT +G T+MDEMAYSINGEN HYGTP+NP A DR+PGGSSSGSAVAV A+L
Sbjct: 66  VVSSLLEAGATALGITIMDEMAYSINGENAHYGTPRNPIAFDRVPGGSSSGSAVAVAARL 125

Query: 125 VDFSLGKFLTVPCFVGTDTGGSIRVPASYCGILGFRPSHGAVSTSGVIPMSQSFDTVGWF 184
           VDFS          +GTDTGGS+RVPASYCGI GFRPSHGAVST G+ PM+QSFDTVGWF
Sbjct: 126 VDFS----------IGTDTGGSVRVPASYCGIFGFRPSHGAVSTVGLTPMAQSFDTVGWF 185

Query: 185 ARDLAVFKRVGRLLLQQSVVELIRPTQVLIAEDCFKLSSIPSKRLTQAFVNSVKKLFGGH 244
           ARD A  KRVG +LLQQ  +  I P+Q++IA+DCFKL S+P   L Q  V SV+K FGG+
Sbjct: 186 ARDTATLKRVGCVLLQQHHLNPIEPSQLIIADDCFKLCSVPHDLLVQPLVGSVEKSFGGN 245

Query: 245 -LIKEISLGNYVDERVPSLKHFMIEENAA--HKHSIPSLAALARSMQLLQRYEFKTNHEE 304
            ++K+++LG Y+ + VPSLKHFM  ++     +  IPSL AL+ SM+LLQR+EFK NH  
Sbjct: 246 TVVKKVNLGEYIGQNVPSLKHFMTSDDVTTQQEFCIPSLMALSSSMRLLQRHEFKINHGA 305

Query: 305 WIRTCNPHFGPGISERISDAMKTTDENTDLSRSIKTELREALAALLEDFGVLAIPTVPGP 364
           WI +  P FGPGISERI +A++T+DE  D  RS+K+EL  AL+ LL + GVL IPTVPGP
Sbjct: 306 WISSVKPEFGPGISERIEEAIRTSDEKIDHCRSVKSELITALSTLLGEKGVLVIPTVPGP 365

Query: 365 PPKLNTNISELHDFRSKAFSLLSIAGVSGFCQALSLSLPPFFSLSLLKVNIPLGLYNGLP 424
           PP L  N++ L  FRS+AFSLLSIAGVSGFCQ                V+IPLGL+  LP
Sbjct: 366 PPHLQANVAALESFRSRAFSLLSIAGVSGFCQ----------------VSIPLGLHENLP 421

Query: 425 VSISLLAKHGADGFLLNVVETI 443
           VS+SL+AK+G+DGFLL++V+++
Sbjct: 426 VSVSLVAKYGSDGFLLSLVDSL 421

BLAST of MS020239 vs. ExPASy Swiss-Prot
Match: Q7XTK3 (Amidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMI1 PE=1 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 4.1e-131
Identity = 241/448 (53.79%), Postives = 311/448 (69.42%), Query Frame = 0

Query: 5   DHGAFVEKFLLQPSSPSDELPLAGLTFAVKDIFDMDGYVTGFGNPEWARTHPPANQTAPT 64
           D+GAF+E+F+L P  PS +LPL GLTFA+KDIFD+ G VTGFGNP+WARTH PA  T+P 
Sbjct: 10  DYGAFMERFVL-PPPPSQQLPLHGLTFAIKDIFDIAGRVTGFGNPDWARTHAPAAATSPV 69

Query: 65  VSTILRGGATCIGRTVMDEMAYSINGENIHYGTPQNPYALDRIPGGSSSGSAVAVGAKLV 124
           V   L  GAT +G T+MDEMAYSINGEN HYGTP NP A  R+PGGSSSGSAVAV A LV
Sbjct: 70  VLAALAAGATSLGTTIMDEMAYSINGENTHYGTPTNPCAPGRVPGGSSSGSAVAVAANLV 129

Query: 125 DFSLGKFLTVPCFVGTDTGGSIRVPASYCGILGFRPSHGAVSTSGVIPMSQSFDTVGWFA 184
           DFSL          GTDTGGS+RVPA+YCGI G RPSHG VS   VIPM+Q FDTVGWF+
Sbjct: 130 DFSL----------GTDTGGSVRVPAAYCGIFGLRPSHGLVSAENVIPMAQMFDTVGWFS 189

Query: 185 RDLAVFKRVGRLLLQQSVVELIRPTQVLIAEDCFKLSSIPSKRLTQAFVNSVKKLFGGHL 244
           RDL+   RV ++LL      + +PTQV I  DCF++      R  Q    SV K F   +
Sbjct: 190 RDLSTLSRVTKVLLPLPDDIVKQPTQVTIPMDCFQILGSLDDRTYQIINASVAKRFDSQI 249

Query: 245 IKEISLGNYVDERVPSLKHFMIEENAAHKHSIPSLAALARSMQLLQRYEFKTNHEEWIRT 304
           +   +LG+++ + VPS+  F+ + + +   S+P+L+ ++  M+ LQR +FK NH EW+ T
Sbjct: 250 LDNRNLGDFISDNVPSIGKFITDFSESELPSVPALSVISHVMRGLQRSQFKANHAEWVNT 309

Query: 305 CNPHFGPGISERISDAMKTTD-ENTDLSRSIKTELREALAALLEDFGVLAIPTVPGPPPK 364
             P+ GPG+ ERI +A+ + D E+ +  ++I+ E + ALAALL+D G+LAIPTVPGPPPK
Sbjct: 310 VKPNLGPGLRERILEAIASGDNESLEDFQAIRAEFKSALAALLKDHGILAIPTVPGPPPK 369

Query: 365 LNTNISELHDFRSKAFSLLSIAGVSGFCQALSLSLPPFFSLSLLKVNIPLGLYNGLPVSI 424
           +    + L +FR++AFSLLSIAG+SGFCQ                V+IPLG+ NGLPVS+
Sbjct: 370 VGMEAAPLENFRARAFSLLSIAGLSGFCQ----------------VSIPLGMRNGLPVSV 429

Query: 425 SLLAKHGADGFLLNVVETIYNTLKEEVS 452
           SL+A+HGAD FLLNVVE +Y TL +E +
Sbjct: 430 SLVARHGADHFLLNVVEELYQTLIDEAT 430

BLAST of MS020239 vs. ExPASy Swiss-Prot
Match: Q9MUK5 (Translocon at the outer membrane of chloroplasts 64 OS=Pisum sativum OX=3888 GN=TOC64 PE=1 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 6.0e-114
Identity = 214/451 (47.45%), Postives = 293/451 (64.97%), Query Frame = 0

Query: 4   QDHGAFVEKFLL----QPSSPSDELPLAGLTFAVKDIFDMDGYVTGFGNPEWARTHPPAN 63
           +D GAF+ K  L     P+ P    PL+ L FA+ DIFD++G+V+ FG+PEWARTH PA+
Sbjct: 35  EDFGAFLLKLKLLPPPPPAPPKAPHPLSSLNFAISDIFDIEGHVSTFGHPEWARTHEPAS 94

Query: 64  QTAPTVSTILRGGATCIGRTVMDEMAYSINGENIHYGTPQNPYALDRIPGGSSSGSAVAV 123
            TA  VS ++  GATCIG TV+DE+AY I+GEN H+GTP NP   +R+PGGSSSG+AVAV
Sbjct: 95  STASAVSALVESGATCIGTTVVDELAYGISGENKHFGTPTNPAVPNRVPGGSSSGAAVAV 154

Query: 124 GAKLVDFSLGKFLTVPCFVGTDTGGSIRVPASYCGILGFRPSHGAVSTSGVIPMSQSFDT 183
            A  VDFSL          G DT G +RVPA +CGILGFRPSHGAVS  G+IP+S S DT
Sbjct: 155 AANFVDFSL----------GVDTSGGVRVPAGFCGILGFRPSHGAVSHVGIIPVSTSLDT 214

Query: 184 VGWFARDLAVFKRVGRLLLQQSVVELIRPTQVLIAEDCFKLSSIPSKRLTQAFVNSVKKL 243
           VGWFA+D  V +RVG +LLQ   V    P Q++IA+DCF+  ++P  R +Q  + + +KL
Sbjct: 215 VGWFAKDPDVLRRVGHILLQAPFVMQRNPRQIIIADDCFQHLNVPLDRTSQVVIKATEKL 274

Query: 244 FGGHLIKEISLGNYVDERVPSLKHFMIEE-NAAHKHSIPSLAALARSMQLLQRYEFKTNH 303
           FG  ++K I+  +Y+  +V SLK   I++ N   K S  SL  LA  MQ LQR+EF+  H
Sbjct: 275 FGKQVLKHINFEDYISSKVSSLKACSIQKSNGVLKSS--SLKLLANVMQSLQRHEFEHTH 334

Query: 304 EEWIRTCNPHFGPGISERISDAMKTTDENTDLSRSIKTELREALAALLEDFGVLAIPTVP 363
            EW+    P   P +S ++ +  + ++   + S+S+++ELR A+ +LL+D GVL IPTV 
Sbjct: 335 SEWMSIVKPDLHPAVSAQLHEKFEVSELEIENSKSVRSELRVAVNSLLKDEGVLVIPTVA 394

Query: 364 GPPPKLNTNISELHDFRSKAFSLLSIAGVSGFCQALSLSLPPFFSLSLLKVNIPLGLYNG 423
            PPPKL       HD++S+A SLLSIA +SG CQ                V +PLG ++ 
Sbjct: 395 DPPPKLGGKEFLSHDYQSRALSLLSIASISGCCQ----------------VTVPLGFFDK 454

Query: 424 LPVSISLLAKHGADGFLLNVVETIYNTLKEE 450
            PVS+SL+A+HG D FLL+ ++T+Y  L+E+
Sbjct: 455 NPVSVSLIARHGGDRFLLDTLKTMYTVLQEQ 457

BLAST of MS020239 vs. ExPASy Swiss-Prot
Match: Q9LVH5 (Outer envelope protein 64, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OEP64 PE=1 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 1.3e-113
Identity = 208/449 (46.33%), Postives = 286/449 (63.70%), Query Frame = 0

Query: 4   QDHGAFVEKFLL----QPSSPSDELPLAGLTFAVKDIFDMDGYVTGFGNPEWARTHPPAN 63
           +D GAF++K +L    QP+ P    PL GLTFAV D+FD+ GYVTGFG+P+W RTH  A+
Sbjct: 32  EDFGAFIDKLMLLPPPQPAPPKAPHPLTGLTFAVSDVFDITGYVTGFGHPDWVRTHEAAS 91

Query: 64  QTAPTVSTILRGGATCIGRTVMDEMAYSINGENIHYGTPQNPYALDRIPGGSSSGSAVAV 123
            T+P VST++ GGATC+G+TV+DE A+SI+GEN HY +P NP A  RIPGG+ SG+AVAV
Sbjct: 92  STSPVVSTLVEGGATCVGKTVVDEFAFSISGENKHYDSPTNPAAPTRIPGGACSGAAVAV 151

Query: 124 GAKLVDFSLGKFLTVPCFVGTDTGGSIRVPASYCGILGFRPSHGAVSTSGVIPMSQSFDT 183
               VDF+L          G DT G +RVPA YCG+LGF+ S+GA+S +G+IP+S S D+
Sbjct: 152 ATNAVDFAL----------GIDTVGGVRVPAGYCGVLGFKSSYGAISNTGIIPVSSSLDS 211

Query: 184 VGWFARDLAVFKRVGRLLLQQSVVELIRPTQVLIAEDCFKLSSIPSKRLTQAFVNSVKKL 243
           VGWFARD    +RVG +LLQ        P Q+++A+DCF+L  IP  R+TQ    S +KL
Sbjct: 212 VGWFARDPNTLRRVGHVLLQLPFATQRNPRQIILADDCFQLLKIPVDRITQVVTKSAEKL 271

Query: 244 FGGHLIKEISLGNYVDERVPSLKHFMIEENAAHKHSIPSLAALARSMQLLQRYEFKTNHE 303
           FG  L+K  +L  Y + +VPSLK F     A     + +   LA  MQLLQR+EF  NH 
Sbjct: 272 FGRQLLKHQNLETYFETKVPSLKEF-ARTKAIANTKVSTSRLLANVMQLLQRHEFLQNHG 331

Query: 304 EWIRTCNPHFGPGISERISDAMKTTDENTDLSRSIKTELREALAALLEDFGVLAIPTVPG 363
           +WI T  P   P I  ++ +  + T+E T+   +I+ E R A+ +LL+D G+L IPT+P 
Sbjct: 332 DWINTVKPAIDPVILSQVCENPELTNEETENLNAIRNETRVAIGSLLKDDGILVIPTLPA 391

Query: 364 PPPKLNTNISELHDFRSKAFSLLSIAGVSGFCQALSLSLPPFFSLSLLKVNIPLGLYNGL 423
            PPKL +      D++++A SLLSIA +SG CQ                V +PLG +   
Sbjct: 392 VPPKLGSKEITSEDYQNRASSLLSIASISGCCQ----------------VTVPLGHHEKC 451

Query: 424 PVSISLLAKHGADGFLLNVVETIYNTLKE 449
           P+S+S + +HG D FLL+ V+T+Y +L+E
Sbjct: 452 PISVSFIGRHGGDRFLLDTVQTMYPSLQE 453

BLAST of MS020239 vs. ExPASy Swiss-Prot
Match: F4KCL7 (Outer envelope protein 64, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OM64 PE=1 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 4.3e-104
Identity = 198/451 (43.90%), Postives = 283/451 (62.75%), Query Frame = 0

Query: 4   QDHGAFVEKFLL----QPSSPSDELPLAGLTFAVKDIFDMDGYVTGFGNPEWARTHPPAN 63
           +D GAF+++F L     P  P+ +  L+GLTF++ D FD+  Y+TGFG P+W +TH  A 
Sbjct: 45  EDFGAFLDRFELLPFPPPPPPAAKQSLSGLTFSISDAFDVKDYITGFGCPQWKKTHEAAE 104

Query: 64  QTAPTVSTILRGGATCIGRTVMDEMAYSINGENIHYGTPQNPYALDRIPGGSSSGSAVAV 123
           +TA  V+T+L+ GATC+G+T+MDE+ + I GEN HYGTP NP   D +PGG SSGSAV+V
Sbjct: 105 KTAVVVTTLLKNGATCVGKTIMDELGFGIIGENKHYGTPINPLMPDNVPGGCSSGSAVSV 164

Query: 124 GAKLVDFSLGKFLTVPCFVGTDTGGSIRVPASYCGILGFRPSHGAVSTSGVIPMSQSFDT 183
           GA+LVDFSL          G DT G +RVPA++CGILGFRPS G VS+ GV+P SQS +T
Sbjct: 165 GAELVDFSL----------GIDTTGGVRVPAAFCGILGFRPSQGTVSSVGVLPNSQSLET 224

Query: 184 VGWFARDLAVFKRVGRLLLQQSVVELIRPTQVLIAEDCFKLSSIPSKRLTQAFVNSVKKL 243
           VGWFA D +V  +VG  LL  S V   R   ++ A+D F+LS IP ++  Q    +++ L
Sbjct: 225 VGWFASDPSVLCQVGHALLNLSAVTHRRQRSLIFADDLFELSDIPKQKSVQVVRKAIENL 284

Query: 244 FGGHLIKEISLGNYVDERVPSLKHFMIEENAAHKHSIPSLAALARSMQLLQRYEFKTNHE 303
            G    K +++G YV   VPSL  F  E++   ++S  +L AL+  M  +QR+EFKTNHE
Sbjct: 285 SGYKTPKHVNVGQYVASNVPSLAEF-CEQSGKSQNSASTLRALSSVMLAIQRHEFKTNHE 344

Query: 304 EWIRTCNPHFGPGISERISDAMKTTDENTDLSRSIKTELREALAALLEDFGVLAIPTVPG 363
           EW +TC    GP  S  +  A+K+ +E+      +K E+R  + +LL++ G+L IPTV  
Sbjct: 345 EWWQTCKSFLGPRFSNDVVTALKSKNESIKSLYRVKNEMRATIQSLLKEDGILVIPTVAD 404

Query: 364 PPPKLNTNISE-LHDFRSKAFSLLSIAGVSGFCQALSLSLPPFFSLSLLKVNIPLGLYNG 423
           PPP+LNT  ++ L++F  + ++L  IA +SG CQ                V IPLG +  
Sbjct: 405 PPPRLNTKRNKSLNEFLDRTYALSCIASMSGCCQ----------------VTIPLGEHGD 464

Query: 424 LPVSISLLAKHGADGFLLNVVETIYNTLKEE 450
            P+S+SLL  +G D FLL+    +Y +L+++
Sbjct: 465 RPISVSLLTYYGGDKFLLDTTLDVYASLQDQ 468

BLAST of MS020239 vs. ExPASy TrEMBL
Match: A0A6J1DR29 (amidase 1 OS=Momordica charantia OX=3673 GN=LOC111023546 PE=3 SV=1)

HSP 1 Score: 834.7 bits (2155), Expect = 1.8e-238
Identity = 425/452 (94.03%), Postives = 425/452 (94.03%), Query Frame = 0

Query: 1   MAVQDHGAFVEKFLLQPSSPSDELPLAGLTFAVKDIFDMDGYVTGFGNPEWARTHPPANQ 60
           MAVQDHGAFVEKFLLQPSSPSDELPLAGLTFAVKDIFDMDGYVTGFGNPEWARTHPPANQ
Sbjct: 1   MAVQDHGAFVEKFLLQPSSPSDELPLAGLTFAVKDIFDMDGYVTGFGNPEWARTHPPANQ 60

Query: 61  TAPTVSTILRGGATCIGRTVMDEMAYSINGENIHYGTPQNPYALDRIPGGSSSGSAVAVG 120
           TAPTVSTILRGGATCIGRTVMDEMAYSINGENIHYGTPQNPYALDRIPGGSSSGSAVAVG
Sbjct: 61  TAPTVSTILRGGATCIGRTVMDEMAYSINGENIHYGTPQNPYALDRIPGGSSSGSAVAVG 120

Query: 121 AKLVDFSLGKFLTVPCFVGTDTGGSIRVPASYCGILGFRPSHGAVSTSGVIPMSQSFDTV 180
           AKLVDFSL          GTDTGGSIRVPASYCGILGFRPSHGAVSTSGVIPMSQSFDTV
Sbjct: 121 AKLVDFSL----------GTDTGGSIRVPASYCGILGFRPSHGAVSTSGVIPMSQSFDTV 180

Query: 181 GWFARDLAVFKRVGRLLLQQSVVELIRPTQVLIAEDCFKLSSIPSKRLTQAFVNSVKKLF 240
           GWFARDLAVFKRVGRLLLQQS VELIRPTQVLIAEDCFKLSSIPSKRLTQAFVNSVKKLF
Sbjct: 181 GWFARDLAVFKRVGRLLLQQSEVELIRPTQVLIAEDCFKLSSIPSKRLTQAFVNSVKKLF 240

Query: 241 GGHLIKEISLGNYVDERVPSLKHFMIEENAAHKHSIPSLAALARSMQLLQRYEFKTNHEE 300
           GGHLIKEISLGNYVDERVPSLKHFMIEENAAHKHSIPSLAALARSMQLLQRYEFKTNHEE
Sbjct: 241 GGHLIKEISLGNYVDERVPSLKHFMIEENAAHKHSIPSLAALARSMQLLQRYEFKTNHEE 300

Query: 301 WIRTCNPHFGPGISERISDAMKTTDENTDLSRSIKTELREALAALLEDFGVLAIPTVPGP 360
           WIRTCNPHFGPGISERISDAMKTTDENTDLSRSIKTELREALAALLEDFGVLAIPTVPGP
Sbjct: 301 WIRTCNPHFGPGISERISDAMKTTDENTDLSRSIKTELREALAALLEDFGVLAIPTVPGP 360

Query: 361 PPKLNTNISELHDFRSKAFSLLSIAGVSGFCQALSLSLPPFFSLSLLKVNIPLGLYNGLP 420
           PPKLNTNISELHDFRSKAFSLLSIAGVSGFCQ                VNIPLGLYNGLP
Sbjct: 361 PPKLNTNISELHDFRSKAFSLLSIAGVSGFCQ----------------VNIPLGLYNGLP 420

Query: 421 VSISLLAKHGADGFLLNVVETIYNTLKEEVSC 453
           VSISLLAKHGADGFLLNVVETIYNTLKEEVSC
Sbjct: 421 VSISLLAKHGADGFLLNVVETIYNTLKEEVSC 426

BLAST of MS020239 vs. ExPASy TrEMBL
Match: A0A6J1HY10 (amidase 1 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111467873 PE=3 SV=1)

HSP 1 Score: 731.1 bits (1886), Expect = 2.8e-207
Identity = 360/450 (80.00%), Postives = 398/450 (88.44%), Query Frame = 0

Query: 1   MAVQDHGAFVEKFLLQPSSPSDELPLAGLTFAVKDIFDMDGYVTGFGNPEWARTHPPANQ 60
           MAVQDHGAF+EKFLLQPSSPSD+LPLAGLTFAVKDIFD+DGYVTGFGNPEW RTHPPANQ
Sbjct: 1   MAVQDHGAFMEKFLLQPSSPSDQLPLAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPANQ 60

Query: 61  TAPTVSTILRGGATCIGRTVMDEMAYSINGENIHYGTPQNPYALDRIPGGSSSGSAVAVG 120
           TAP VS+IL+GGATCIG+T+MDEMAYSINGENIHYGTPQNP+A DRIPGGSSSGSAVAVG
Sbjct: 61  TAPAVSSILKGGATCIGKTIMDEMAYSINGENIHYGTPQNPHASDRIPGGSSSGSAVAVG 120

Query: 121 AKLVDFSLGKFLTVPCFVGTDTGGSIRVPASYCGILGFRPSHGAVSTSGVIPMSQSFDTV 180
           AKLVDFSL          GTDTGGS+RVPASYCGI GFRPSHGAVSTSGVIPM+QSFDTV
Sbjct: 121 AKLVDFSL----------GTDTGGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTV 180

Query: 181 GWFARDLAVFKRVGRLLLQQSVVELIRPTQVLIAEDCFKLSSIPSKRLTQAFVNSVKKLF 240
           GWFARD  V KRVG+LLLQQ   E  +PTQVLIAEDC KLS IPS+RLTQ FVN+V KLF
Sbjct: 181 GWFARDPIVLKRVGQLLLQQPEFEHHKPTQVLIAEDCMKLSCIPSERLTQVFVNAVTKLF 240

Query: 241 GGHLIKEISLGNYVDERVPSLKHFMIEENAAHKHSIPSLAALARSMQLLQRYEFKTNHEE 300
           GGHLIK++SLGNYV+++VPSLKHFMIE NA H+HSIPSLAALARSMQLLQRYEFK NHEE
Sbjct: 241 GGHLIKQVSLGNYVEDKVPSLKHFMIEGNAGHEHSIPSLAALARSMQLLQRYEFKINHEE 300

Query: 301 WIRTCNPHFGPGISERISDAMKTTDENTDLSRSIKTELREALAALLEDFGVLAIPTVPGP 360
           W+RTCNPH GPGISER+ +AM+TTDEN DL RSIK +LREALA+LLEDFGVL IPTVPGP
Sbjct: 301 WVRTCNPHLGPGISERVLEAMRTTDENIDLCRSIKVKLREALASLLEDFGVLVIPTVPGP 360

Query: 361 PPKLNTNISELHDFRSKAFSLLSIAGVSGFCQALSLSLPPFFSLSLLKVNIPLGLYNGLP 420
           PP+LNT++S+LHDFR+KAFSLLSIAGVSG CQ                V+IPLGLYNGLP
Sbjct: 361 PPELNTDVSKLHDFRAKAFSLLSIAGVSGLCQ----------------VSIPLGLYNGLP 420

Query: 421 VSISLLAKHGADGFLLNVVETIYNTLKEEV 451
           VS+SLLAKHG+DGFLLNVV+++YNTLK+EV
Sbjct: 421 VSLSLLAKHGSDGFLLNVVDSLYNTLKQEV 424

BLAST of MS020239 vs. ExPASy TrEMBL
Match: A0A5D3CG55 (Amidase 1-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003790 PE=4 SV=1)

HSP 1 Score: 729.2 bits (1881), Expect = 1.1e-206
Identity = 365/450 (81.11%), Postives = 398/450 (88.44%), Query Frame = 0

Query: 1   MAVQDHGAFVEKFLLQPSSPSDELPLAGLTFAVKDIFDMDGYVTGFGNPEWARTHPPANQ 60
           MAVQ +GAF+EKFLLQPSSPSD+LPL GLTFAVKDIFDMDG+VTGFGNPEW RTHPPAN 
Sbjct: 46  MAVQ-YGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 105

Query: 61  TAPTVSTILRGGATCIGRTVMDEMAYSINGENIHYGTPQNPYALDRIPGGSSSGSAVAVG 120
           TAPTVSTILRGGATCIGRT+MDEMAYSINGEN HYGTPQNP A DR+PGGSSSGSAVAVG
Sbjct: 106 TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 165

Query: 121 AKLVDFSLGKFLTVPCFVGTDTGGSIRVPASYCGILGFRPSHGAVSTSGVIPMSQSFDTV 180
           A LVDFSLG       FVGTDTGGS+RVPASYCGILGFRPSHGAVSTSGV+PM+QSFDTV
Sbjct: 166 ANLVDFSLG------TFVGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTV 225

Query: 181 GWFARDLAVFKRVGRLLLQQSVVELIRPTQVLIAEDCFKLSSIPSKRLTQAFVNSVKKLF 240
           GWFARD  VFK+VG LLLQQ  VE  +PTQVLIAEDCFKLSSIPS+RLTQAFVNSVKKLF
Sbjct: 226 GWFARDPVVFKKVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLF 285

Query: 241 GGHLIKEISLGNYVDERVPSLKHFMIEENAAHKHSIPSLAALARSMQLLQRYEFKTNHEE 300
           GGHLIK+++LGNYV+E+VPSLK FMIE NA ++HSIPSLAALARS+QLLQRYEFK NHEE
Sbjct: 286 GGHLIKQVNLGNYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEE 345

Query: 301 WIRTCNPHFGPGISERISDAMKTTDENTDLSRSIKTELREALAALLEDFGVLAIPTVPGP 360
           W+RT NPH GPGISER+S+AM+ TDEN DL  SIK ELR ALAALLEDFGVLAIPTVPGP
Sbjct: 346 WVRTYNPHLGPGISERVSEAMRATDENIDLCHSIKIELRRALAALLEDFGVLAIPTVPGP 405

Query: 361 PPKLNTNISELHDFRSKAFSLLSIAGVSGFCQALSLSLPPFFSLSLLKVNIPLGLYNGLP 420
           PPKLNT++SELHDFR+KAFSLLSIAGVSGFCQ                V+IPLGLYNGLP
Sbjct: 406 PPKLNTDVSELHDFRAKAFSLLSIAGVSGFCQ----------------VSIPLGLYNGLP 465

Query: 421 VSISLLAKHGADGFLLNVVETIYNTLKEEV 451
           VSISL+A HG+DGFLLNVV ++YNTL+EEV
Sbjct: 466 VSISLVANHGSDGFLLNVVHSLYNTLEEEV 472

BLAST of MS020239 vs. ExPASy TrEMBL
Match: A0A6J1HVP2 (amidase 1 isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC111467873 PE=3 SV=1)

HSP 1 Score: 726.1 bits (1873), Expect = 9.1e-206
Identity = 358/450 (79.56%), Postives = 396/450 (88.00%), Query Frame = 0

Query: 1   MAVQDHGAFVEKFLLQPSSPSDELPLAGLTFAVKDIFDMDGYVTGFGNPEWARTHPPANQ 60
           MAVQDHGAF+EKF+LQ SSPSD LPLAGLTFAVKDIFD+DGYVTGFGNPEW RTHPPANQ
Sbjct: 1   MAVQDHGAFIEKFILQLSSPSDPLPLAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPANQ 60

Query: 61  TAPTVSTILRGGATCIGRTVMDEMAYSINGENIHYGTPQNPYALDRIPGGSSSGSAVAVG 120
           TAP VS+IL+GGATCIG+T+MDEMAYSINGENIHYGTPQNP+A DRIPGGSSSGSAVAVG
Sbjct: 61  TAPAVSSILKGGATCIGKTIMDEMAYSINGENIHYGTPQNPHASDRIPGGSSSGSAVAVG 120

Query: 121 AKLVDFSLGKFLTVPCFVGTDTGGSIRVPASYCGILGFRPSHGAVSTSGVIPMSQSFDTV 180
           AKLVDFSL          GTDTGGS+RVPASYCGI GFRPSHGAVSTSGVIPM+QSFDTV
Sbjct: 121 AKLVDFSL----------GTDTGGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTV 180

Query: 181 GWFARDLAVFKRVGRLLLQQSVVELIRPTQVLIAEDCFKLSSIPSKRLTQAFVNSVKKLF 240
           GWFARD  V KRVG+LLLQQ   E  +PTQVLIAEDC KLS IPS+RLTQ FVN+V KLF
Sbjct: 181 GWFARDPIVLKRVGQLLLQQPEFEHHKPTQVLIAEDCMKLSCIPSERLTQVFVNAVTKLF 240

Query: 241 GGHLIKEISLGNYVDERVPSLKHFMIEENAAHKHSIPSLAALARSMQLLQRYEFKTNHEE 300
           GGHLIK++SLGNYV+++VPSLKHFMIE NA H+HSIPSLAALARSMQLLQRYEFK NHEE
Sbjct: 241 GGHLIKQVSLGNYVEDKVPSLKHFMIEGNAGHEHSIPSLAALARSMQLLQRYEFKINHEE 300

Query: 301 WIRTCNPHFGPGISERISDAMKTTDENTDLSRSIKTELREALAALLEDFGVLAIPTVPGP 360
           W+RTCNPH GPGISER+ +AM+TTDEN DL RSIK +LREALA+LLEDFGVL IPTVPGP
Sbjct: 301 WVRTCNPHLGPGISERVLEAMRTTDENIDLCRSIKVKLREALASLLEDFGVLVIPTVPGP 360

Query: 361 PPKLNTNISELHDFRSKAFSLLSIAGVSGFCQALSLSLPPFFSLSLLKVNIPLGLYNGLP 420
           PP+LNT++S+LHDFR+KAFSLLSIAGVSG CQ                V+IPLGLYNGLP
Sbjct: 361 PPELNTDVSKLHDFRAKAFSLLSIAGVSGLCQ----------------VSIPLGLYNGLP 420

Query: 421 VSISLLAKHGADGFLLNVVETIYNTLKEEV 451
           VS+SLLAKHG+DGFLLNVV+++YNTLK+EV
Sbjct: 421 VSLSLLAKHGSDGFLLNVVDSLYNTLKQEV 424

BLAST of MS020239 vs. ExPASy TrEMBL
Match: A0A5A7U6R8 (Amidase 1-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold761G00050 PE=4 SV=1)

HSP 1 Score: 726.1 bits (1873), Expect = 9.1e-206
Identity = 364/450 (80.89%), Postives = 397/450 (88.22%), Query Frame = 0

Query: 1   MAVQDHGAFVEKFLLQPSSPSDELPLAGLTFAVKDIFDMDGYVTGFGNPEWARTHPPANQ 60
           MAVQ +GAF+EKFLLQPSSPSD+LPL GLTFAVKDIFDMDG+VTGFGNPEW RTHPPAN 
Sbjct: 46  MAVQ-YGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 105

Query: 61  TAPTVSTILRGGATCIGRTVMDEMAYSINGENIHYGTPQNPYALDRIPGGSSSGSAVAVG 120
           TAPTVSTILRGGATCIGRT+MDEMAYSINGEN HYGTPQNP A DR+PGGSSSGSAVAVG
Sbjct: 106 TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 165

Query: 121 AKLVDFSLGKFLTVPCFVGTDTGGSIRVPASYCGILGFRPSHGAVSTSGVIPMSQSFDTV 180
           A LVDFSLG       FVGTDTGGS+RVPASYCGILGFRPSHGAVSTSGV+PM+QSFDTV
Sbjct: 166 ANLVDFSLG------TFVGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTV 225

Query: 181 GWFARDLAVFKRVGRLLLQQSVVELIRPTQVLIAEDCFKLSSIPSKRLTQAFVNSVKKLF 240
           GWFARD  VFK+VG LLLQQ  VE  +PTQVLIAEDCFKLSSIPS+RLTQAFVNSVKKLF
Sbjct: 226 GWFARDPVVFKKVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLF 285

Query: 241 GGHLIKEISLGNYVDERVPSLKHFMIEENAAHKHSIPSLAALARSMQLLQRYEFKTNHEE 300
           GGHLIK+++LGNYV+E+VPSLK FMIE NA ++HSIPSLAALARS+QLLQRYEFK NHEE
Sbjct: 286 GGHLIKQVNLGNYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEE 345

Query: 301 WIRTCNPHFGPGISERISDAMKTTDENTDLSRSIKTELREALAALLEDFGVLAIPTVPGP 360
           W+RT NPH GPGISER+S+AM+ TDEN DL  SIK ELR ALAALLEDFGVLAIPTVPGP
Sbjct: 346 WVRTYNPHLGPGISERVSEAMRATDENIDLCHSIKIELRRALAALLEDFGVLAIPTVPGP 405

Query: 361 PPKLNTNISELHDFRSKAFSLLSIAGVSGFCQALSLSLPPFFSLSLLKVNIPLGLYNGLP 420
           PPKLNT++SELHDFR+KAFSLLSIAGVSGFCQ                V+IPL LYNGLP
Sbjct: 406 PPKLNTDVSELHDFRAKAFSLLSIAGVSGFCQ----------------VSIPLRLYNGLP 465

Query: 421 VSISLLAKHGADGFLLNVVETIYNTLKEEV 451
           VSISL+A HG+DGFLLNVV ++YNTL+EEV
Sbjct: 466 VSISLVANHGSDGFLLNVVHSLYNTLEEEV 472

BLAST of MS020239 vs. TAIR 10
Match: AT1G08980.1 (amidase 1 )

HSP 1 Score: 526.2 bits (1354), Expect = 2.7e-149
Identity = 266/442 (60.18%), Postives = 329/442 (74.43%), Query Frame = 0

Query: 5   DHGAFVEKFLLQPSSPSDELP-LAGLTFAVKDIFDMDGYVTGFGNPEWARTHPPANQTAP 64
           D GAF+EK  + P+S S   P L GLTFA+KDIFD++G VTGFGNP+W RTH  A  TAP
Sbjct: 6   DFGAFIEKVTISPTSTSSSPPSLQGLTFAIKDIFDVEGRVTGFGNPDWLRTHSAATSTAP 65

Query: 65  TVSTILRGGATCIGRTVMDEMAYSINGENIHYGTPQNPYALDRIPGGSSSGSAVAVGAKL 124
            VS++L  GAT +G T+MDEMAYSINGEN HYGTP+NP A DR+PGGSSSGSAVAV A+L
Sbjct: 66  VVSSLLEAGATALGITIMDEMAYSINGENAHYGTPRNPIAFDRVPGGSSSGSAVAVAARL 125

Query: 125 VDFSLGKFLTVPCFVGTDTGGSIRVPASYCGILGFRPSHGAVSTSGVIPMSQSFDTVGWF 184
           VDFS          +GTDTGGS+RVPASYCGI GFRPSHGAVST G+ PM+QSFDTVGWF
Sbjct: 126 VDFS----------IGTDTGGSVRVPASYCGIFGFRPSHGAVSTVGLTPMAQSFDTVGWF 185

Query: 185 ARDLAVFKRVGRLLLQQSVVELIRPTQVLIAEDCFKLSSIPSKRLTQAFVNSVKKLFGGH 244
           ARD A  KRVG +LLQQ  +  I P+Q++IA+DCFKL S+P   L Q  V SV+K FGG+
Sbjct: 186 ARDTATLKRVGCVLLQQHHLNPIEPSQLIIADDCFKLCSVPHDLLVQPLVGSVEKSFGGN 245

Query: 245 -LIKEISLGNYVDERVPSLKHFMIEENAA--HKHSIPSLAALARSMQLLQRYEFKTNHEE 304
            ++K+++LG Y+ + VPSLKHFM  ++     +  IPSL AL+ SM+LLQR+EFK NH  
Sbjct: 246 TVVKKVNLGEYIGQNVPSLKHFMTSDDVTTQQEFCIPSLMALSSSMRLLQRHEFKINHGA 305

Query: 305 WIRTCNPHFGPGISERISDAMKTTDENTDLSRSIKTELREALAALLEDFGVLAIPTVPGP 364
           WI +  P FGPGISERI +A++T+DE  D  RS+K+EL  AL+ LL + GVL IPTVPGP
Sbjct: 306 WISSVKPEFGPGISERIEEAIRTSDEKIDHCRSVKSELITALSTLLGEKGVLVIPTVPGP 365

Query: 365 PPKLNTNISELHDFRSKAFSLLSIAGVSGFCQALSLSLPPFFSLSLLKVNIPLGLYNGLP 424
           PP L  N++ L  FRS+AFSLLSIAGVSGFCQ                V+IPLGL+  LP
Sbjct: 366 PPHLQANVAALESFRSRAFSLLSIAGVSGFCQ----------------VSIPLGLHENLP 421

Query: 425 VSISLLAKHGADGFLLNVVETI 443
           VS+SL+AK+G+DGFLL++V+++
Sbjct: 426 VSVSLVAKYGSDGFLLSLVDSL 421

BLAST of MS020239 vs. TAIR 10
Match: AT3G17970.1 (translocon at the outer membrane of chloroplasts 64-III )

HSP 1 Score: 411.4 bits (1056), Expect = 9.5e-115
Identity = 208/449 (46.33%), Postives = 286/449 (63.70%), Query Frame = 0

Query: 4   QDHGAFVEKFLL----QPSSPSDELPLAGLTFAVKDIFDMDGYVTGFGNPEWARTHPPAN 63
           +D GAF++K +L    QP+ P    PL GLTFAV D+FD+ GYVTGFG+P+W RTH  A+
Sbjct: 32  EDFGAFIDKLMLLPPPQPAPPKAPHPLTGLTFAVSDVFDITGYVTGFGHPDWVRTHEAAS 91

Query: 64  QTAPTVSTILRGGATCIGRTVMDEMAYSINGENIHYGTPQNPYALDRIPGGSSSGSAVAV 123
            T+P VST++ GGATC+G+TV+DE A+SI+GEN HY +P NP A  RIPGG+ SG+AVAV
Sbjct: 92  STSPVVSTLVEGGATCVGKTVVDEFAFSISGENKHYDSPTNPAAPTRIPGGACSGAAVAV 151

Query: 124 GAKLVDFSLGKFLTVPCFVGTDTGGSIRVPASYCGILGFRPSHGAVSTSGVIPMSQSFDT 183
               VDF+L          G DT G +RVPA YCG+LGF+ S+GA+S +G+IP+S S D+
Sbjct: 152 ATNAVDFAL----------GIDTVGGVRVPAGYCGVLGFKSSYGAISNTGIIPVSSSLDS 211

Query: 184 VGWFARDLAVFKRVGRLLLQQSVVELIRPTQVLIAEDCFKLSSIPSKRLTQAFVNSVKKL 243
           VGWFARD    +RVG +LLQ        P Q+++A+DCF+L  IP  R+TQ    S +KL
Sbjct: 212 VGWFARDPNTLRRVGHVLLQLPFATQRNPRQIILADDCFQLLKIPVDRITQVVTKSAEKL 271

Query: 244 FGGHLIKEISLGNYVDERVPSLKHFMIEENAAHKHSIPSLAALARSMQLLQRYEFKTNHE 303
           FG  L+K  +L  Y + +VPSLK F     A     + +   LA  MQLLQR+EF  NH 
Sbjct: 272 FGRQLLKHQNLETYFETKVPSLKEF-ARTKAIANTKVSTSRLLANVMQLLQRHEFLQNHG 331

Query: 304 EWIRTCNPHFGPGISERISDAMKTTDENTDLSRSIKTELREALAALLEDFGVLAIPTVPG 363
           +WI T  P   P I  ++ +  + T+E T+   +I+ E R A+ +LL+D G+L IPT+P 
Sbjct: 332 DWINTVKPAIDPVILSQVCENPELTNEETENLNAIRNETRVAIGSLLKDDGILVIPTLPA 391

Query: 364 PPPKLNTNISELHDFRSKAFSLLSIAGVSGFCQALSLSLPPFFSLSLLKVNIPLGLYNGL 423
            PPKL +      D++++A SLLSIA +SG CQ                V +PLG +   
Sbjct: 392 VPPKLGSKEITSEDYQNRASSLLSIASISGCCQ----------------VTVPLGHHEKC 451

Query: 424 PVSISLLAKHGADGFLLNVVETIYNTLKE 449
           P+S+S + +HG D FLL+ V+T+Y +L+E
Sbjct: 452 PISVSFIGRHGGDRFLLDTVQTMYPSLQE 453

BLAST of MS020239 vs. TAIR 10
Match: AT5G09420.1 (translocon at the outer membrane of chloroplasts 64-V )

HSP 1 Score: 379.8 bits (974), Expect = 3.1e-105
Identity = 198/451 (43.90%), Postives = 283/451 (62.75%), Query Frame = 0

Query: 4   QDHGAFVEKFLL----QPSSPSDELPLAGLTFAVKDIFDMDGYVTGFGNPEWARTHPPAN 63
           +D GAF+++F L     P  P+ +  L+GLTF++ D FD+  Y+TGFG P+W +TH  A 
Sbjct: 45  EDFGAFLDRFELLPFPPPPPPAAKQSLSGLTFSISDAFDVKDYITGFGCPQWKKTHEAAE 104

Query: 64  QTAPTVSTILRGGATCIGRTVMDEMAYSINGENIHYGTPQNPYALDRIPGGSSSGSAVAV 123
           +TA  V+T+L+ GATC+G+T+MDE+ + I GEN HYGTP NP   D +PGG SSGSAV+V
Sbjct: 105 KTAVVVTTLLKNGATCVGKTIMDELGFGIIGENKHYGTPINPLMPDNVPGGCSSGSAVSV 164

Query: 124 GAKLVDFSLGKFLTVPCFVGTDTGGSIRVPASYCGILGFRPSHGAVSTSGVIPMSQSFDT 183
           GA+LVDFSL          G DT G +RVPA++CGILGFRPS G VS+ GV+P SQS +T
Sbjct: 165 GAELVDFSL----------GIDTTGGVRVPAAFCGILGFRPSQGTVSSVGVLPNSQSLET 224

Query: 184 VGWFARDLAVFKRVGRLLLQQSVVELIRPTQVLIAEDCFKLSSIPSKRLTQAFVNSVKKL 243
           VGWFA D +V  +VG  LL  S V   R   ++ A+D F+LS IP ++  Q    +++ L
Sbjct: 225 VGWFASDPSVLCQVGHALLNLSAVTHRRQRSLIFADDLFELSDIPKQKSVQVVRKAIENL 284

Query: 244 FGGHLIKEISLGNYVDERVPSLKHFMIEENAAHKHSIPSLAALARSMQLLQRYEFKTNHE 303
            G    K +++G YV   VPSL  F  E++   ++S  +L AL+  M  +QR+EFKTNHE
Sbjct: 285 SGYKTPKHVNVGQYVASNVPSLAEF-CEQSGKSQNSASTLRALSSVMLAIQRHEFKTNHE 344

Query: 304 EWIRTCNPHFGPGISERISDAMKTTDENTDLSRSIKTELREALAALLEDFGVLAIPTVPG 363
           EW +TC    GP  S  +  A+K+ +E+      +K E+R  + +LL++ G+L IPTV  
Sbjct: 345 EWWQTCKSFLGPRFSNDVVTALKSKNESIKSLYRVKNEMRATIQSLLKEDGILVIPTVAD 404

Query: 364 PPPKLNTNISE-LHDFRSKAFSLLSIAGVSGFCQALSLSLPPFFSLSLLKVNIPLGLYNG 423
           PPP+LNT  ++ L++F  + ++L  IA +SG CQ                V IPLG +  
Sbjct: 405 PPPRLNTKRNKSLNEFLDRTYALSCIASMSGCCQ----------------VTIPLGEHGD 464

Query: 424 LPVSISLLAKHGADGFLLNVVETIYNTLKEE 450
            P+S+SLL  +G D FLL+    +Y +L+++
Sbjct: 465 RPISVSLLTYYGGDKFLLDTTLDVYASLQDQ 468

BLAST of MS020239 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 104.0 bits (258), Expect = 3.3e-22
Identity = 61/164 (37.20%), Postives = 84/164 (51.22%), Query Frame = 0

Query: 25  PLAGLTFAVKDIFDMDGYVTGFGNPEWARTHPPANQTAPTVSTILRGGATCIGRTVMDEM 84
           PLAG+   VKD     G  +   +       PP + TA  V  I   G   +G+T MDE 
Sbjct: 107 PLAGVLIGVKDNICTQGMPSTAASRILEHYRPPFDATA--VKKIKELGGIVVGKTNMDEF 166

Query: 85  AYSINGENIHYGTPQNPYALDRIPGGSSSGSAVAVGAKLVDFSLGKFLTVPCFVGTDTGG 144
                 E   +    NP+ L R+PGGSS GSA AV A+    SL          G+DTGG
Sbjct: 167 GMGSTTEASAFQVTANPWDLSRVPGGSSGGSAAAVAARQCMVSL----------GSDTGG 226

Query: 145 SIRVPASYCGILGFRPSHGAVSTSGVIPMSQSFDTVGWFARDLA 189
           S+R PAS+CG++G +P++G VS  G++  + S D +G F   +A
Sbjct: 227 SVRQPASFCGVVGLKPTYGRVSRFGLMAYASSLDVIGCFGSTVA 258

BLAST of MS020239 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 87.0 bits (214), Expect = 4.1e-17
Identity = 52/162 (32.10%), Postives = 77/162 (47.53%), Query Frame = 0

Query: 26  LAGLTFAVKDIFDMDGYVTGFGNPEWARTHPPANQTAPTVSTILRGGATCIGRTVMDEMA 85
           L G+   +KD  D   + T  G   W        + +  VS +   GA  +G+  M E+ 
Sbjct: 197 LDGIFVTIKDDIDCLPHPTN-GGTTWLHEDRSVEKDSAVVSKLRSCGAILLGKANMHELG 256

Query: 86  YSINGENIHYGTPQNPYALDRIPGGSSSGSAVAVGAKLVDFSLGKFLTVPCFVGTDTGGS 145
               G N +YGT +NP+   R  GGSSSGSA  V A L   +L          GTD GGS
Sbjct: 257 MGTTGNNSNYGTTRNPHDPKRYTGGSSSGSAAIVAAGLCSAAL----------GTDGGGS 316

Query: 146 IRVPASYCGILGFRPSHGAVSTSGVIPMSQSFDTVGWFARDL 188
           +R+P++ CGI G + ++G    +G +    + + +G  A  L
Sbjct: 317 VRIPSALCGITGLKTTYGRTDMTGSLCEGGTVEIIGPLASSL 347