MS020087 (gene) Bitter gourd (TR) v1

Overview
NameMS020087
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionE3 ubiquitin-protein ligase CIP8-like
Locationscaffold22: 1613182 .. 1613823 (-)
RNA-Seq ExpressionMS020087
SyntenyMS020087
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGGCCGCCGGCCTCCACTGCGAGGTGTGGCAGCCCCAAGAGTGGGCCGAATCAGACTGCTCCTCCTCCTCTTCCGAACCCCCCGAATTCTTCATCGATCTGTACGTCTCCTCCTACGCAAATCAAGGGCGCGAAACAATCAGCAGTCTCCGATACAAGAATTTCCGCCGGCGACGCGACGTTTTCACCCAAGATTCCTTGTCCTGGTCCGCAATTTCCACCATGCTCTCCGAAACCAGCGTGCCCTACCATCTCCAGCCCTTCTTCATCCAACACATTTCCCTTAGGGCTCGCCGAATCGCCGCCCAGCCCACCAACGCCCTCTCCCGCACCATCCCCATCGTCGTCGAGCTCGTCCTGCCACTGCCGGAGGAGGAGGAATCCGGTTCCGGGTCGGATCCCCGGGTTCCGAACGGGCCGGGCCGGGCGAGTAGTAGGGCTTCGATTGAGGAGATGGAGAGGGCGGAAATCGAATTGGGGATTTGTGGCGGTGATTGCGCCATCTGTTTGGATGAGATTGAATGTGGCGCCGTTGGGATGCCCTGTTCGCATATTTACCATCGGAATTGCATTGTGAAATGGCTGCAGCAGAACAATCTTTGCCCCTTGTGTCGGTTTCAGATGCCACTGGAAATGGAA

mRNA sequence

ATGTCGGCCGCCGGCCTCCACTGCGAGGTGTGGCAGCCCCAAGAGTGGGCCGAATCAGACTGCTCCTCCTCCTCTTCCGAACCCCCCGAATTCTTCATCGATCTGTACGTCTCCTCCTACGCAAATCAAGGGCGCGAAACAATCAGCAGTCTCCGATACAAGAATTTCCGCCGGCGACGCGACGTTTTCACCCAAGATTCCTTGTCCTGGTCCGCAATTTCCACCATGCTCTCCGAAACCAGCGTGCCCTACCATCTCCAGCCCTTCTTCATCCAACACATTTCCCTTAGGGCTCGCCGAATCGCCGCCCAGCCCACCAACGCCCTCTCCCGCACCATCCCCATCGTCGTCGAGCTCGTCCTGCCACTGCCGGAGGAGGAGGAATCCGGTTCCGGGTCGGATCCCCGGGTTCCGAACGGGCCGGGCCGGGCGAGTAGTAGGGCTTCGATTGAGGAGATGGAGAGGGCGGAAATCGAATTGGGGATTTGTGGCGGTGATTGCGCCATCTGTTTGGATGAGATTGAATGTGGCGCCGTTGGGATGCCCTGTTCGCATATTTACCATCGGAATTGCATTGTGAAATGGCTGCAGCAGAACAATCTTTGCCCCTTGTGTCGGTTTCAGATGCCACTGGAAATGGAA

Coding sequence (CDS)

ATGTCGGCCGCCGGCCTCCACTGCGAGGTGTGGCAGCCCCAAGAGTGGGCCGAATCAGACTGCTCCTCCTCCTCTTCCGAACCCCCCGAATTCTTCATCGATCTGTACGTCTCCTCCTACGCAAATCAAGGGCGCGAAACAATCAGCAGTCTCCGATACAAGAATTTCCGCCGGCGACGCGACGTTTTCACCCAAGATTCCTTGTCCTGGTCCGCAATTTCCACCATGCTCTCCGAAACCAGCGTGCCCTACCATCTCCAGCCCTTCTTCATCCAACACATTTCCCTTAGGGCTCGCCGAATCGCCGCCCAGCCCACCAACGCCCTCTCCCGCACCATCCCCATCGTCGTCGAGCTCGTCCTGCCACTGCCGGAGGAGGAGGAATCCGGTTCCGGGTCGGATCCCCGGGTTCCGAACGGGCCGGGCCGGGCGAGTAGTAGGGCTTCGATTGAGGAGATGGAGAGGGCGGAAATCGAATTGGGGATTTGTGGCGGTGATTGCGCCATCTGTTTGGATGAGATTGAATGTGGCGCCGTTGGGATGCCCTGTTCGCATATTTACCATCGGAATTGCATTGTGAAATGGCTGCAGCAGAACAATCTTTGCCCCTTGTGTCGGTTTCAGATGCCACTGGAAATGGAA

Protein sequence

MSAAGLHCEVWQPQEWAESDCSSSSSEPPEFFIDLYVSSYANQGRETISSLRYKNFRRRRDVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVELVLPLPEEEESGSGSDPRVPNGPGRASSRASIEEMERAEIELGICGGDCAICLDEIECGAVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME
Homology
BLAST of MS020087 vs. NCBI nr
Match: XP_022137468.1 (E3 ubiquitin-protein ligase CIP8-like [Momordica charantia])

HSP 1 Score: 430.6 bits (1106), Expect = 7.7e-117
Identity = 212/214 (99.07%), Postives = 212/214 (99.07%), Query Frame = 0

Query: 1   MSAAGLHCEVWQPQEWAESDCSSSSSEPPEFFIDLYVSSYANQGRETISSLRYKNFRRRR 60
           MSAAGLHCEVWQPQEWAESDCSSSSSEPPEFFIDLYVSSYANQG ETISSLRYKNFRRRR
Sbjct: 1   MSAAGLHCEVWQPQEWAESDCSSSSSEPPEFFIDLYVSSYANQGGETISSLRYKNFRRRR 60

Query: 61  DVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVELV 120
           DVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVELV
Sbjct: 61  DVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVELV 120

Query: 121 LPLPEEEESGSGSDPRVPNGPGRASSRASIEEMERAEIELGICGGDCAICLDEIECGAVG 180
           LPLPEEEESGSGSDPRVPNGPGRA SRASIEEMERAEIELGICGGDCAICLDEIECGAVG
Sbjct: 121 LPLPEEEESGSGSDPRVPNGPGRA-SRASIEEMERAEIELGICGGDCAICLDEIECGAVG 180

Query: 181 MPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME 215
           MPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME
Sbjct: 181 MPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME 213

BLAST of MS020087 vs. NCBI nr
Match: KAG6584266.1 (E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 282.3 bits (721), Expect = 3.4e-72
Identity = 151/220 (68.64%), Postives = 172/220 (78.18%), Query Frame = 0

Query: 2   SAAGLHCEVWQPQEWAESDC--SSSSSEPPEFFIDLYVSSYANQGRETISSLRYKNFRRR 61
           + +GLHCEVWQPQ+++E+ C  SSSSSE PEFFID YVSSYA QG + IS+LRYKNFR+R
Sbjct: 9   AVSGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTLRYKNFRQR 68

Query: 62  RDVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVEL 121
            DV TQDS SWSAISTMLSET+VPYHLQPFFIQ IS+ AR IA +PTNALSRTIP+VVEL
Sbjct: 69  CDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSRTIPMVVEL 128

Query: 122 VLPLPEEEESGSGSDPRVPNGPGRASSRASIEEMERAEIELGICGGDCAICLDEI----- 181
           VLP    E+SG GSD  +  G  RA SRASI+EMER E+  G+   DC ICLD+I     
Sbjct: 129 VLPEEAMEDSGYGSDTIMGTGSRRA-SRASIQEMERVEMN-GVL-SDCVICLDDIVSVGS 188

Query: 182 ECGAVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME 215
           E  A+ MPC HIYHRNCI KWLQ +N CPLCRFQMPLE E
Sbjct: 189 EIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225

BLAST of MS020087 vs. NCBI nr
Match: XP_008437461.1 (PREDICTED: uncharacterized protein LOC103482872 [Cucumis melo] >TYJ99097.1 E3 ubiquitin-protein ligase [Cucumis melo var. makuwa])

HSP 1 Score: 281.2 bits (718), Expect = 7.6e-72
Identity = 153/226 (67.70%), Postives = 173/226 (76.55%), Query Frame = 0

Query: 1   MSAA----GLHCEVWQPQEWAESDCSSSSS---EPPEFFIDLYVSSYANQGRETISSLRY 60
           MSAA    GLHCEVWQPQ+++E  CSSSSS   +PPEFFID YVSSYA  G E IS+LRY
Sbjct: 1   MSAAAAISGLHCEVWQPQDFSEPSCSSSSSSQFQPPEFFIDFYVSSYATHGDEVISTLRY 60

Query: 61  KNFRRRRDVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTI 120
           KNFR+R DV TQDS SWSAIS+MLSET+VPYHLQPFFI  IS+RAR IA +P NALSRTI
Sbjct: 61  KNFRQRCDVLTQDSFSWSAISSMLSETNVPYHLQPFFIHQISIRARGIATEPINALSRTI 120

Query: 121 PIVVELVLPLPEEEESGSGSDPRVPNGPGRASSRASIEEMERAEIELGICGGDCAICLDE 180
           P+VVEL+LP    E SG GS+P +  G GRA SRASI+EMER EI+ G+   DC ICLDE
Sbjct: 121 PMVVELMLPEDAMEGSGYGSEPHMGIGSGRA-SRASIQEMERIEID-GVL-SDCVICLDE 180

Query: 181 I-----ECGAVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME 215
           I     E  AV MPC H+YH NCI KWL+ +N CPLCRFQMPLE E
Sbjct: 181 IGSIGCEIDAVRMPCLHVYHLNCIHKWLELSNRCPLCRFQMPLEEE 223

BLAST of MS020087 vs. NCBI nr
Match: XP_023001523.1 (uncharacterized protein LOC111495638 [Cucurbita maxima])

HSP 1 Score: 280.8 bits (717), Expect = 9.9e-72
Identity = 149/220 (67.73%), Postives = 170/220 (77.27%), Query Frame = 0

Query: 2   SAAGLHCEVWQPQEWAESDC--SSSSSEPPEFFIDLYVSSYANQGRETISSLRYKNFRRR 61
           + +GLHCEVWQPQ+++E+ C  SSSSSE PEFFID YVSSYA QG + IS+LRYKNFR+R
Sbjct: 9   AVSGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTLRYKNFRQR 68

Query: 62  RDVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVEL 121
            DV TQDS SWSAISTMLSET+VPYHLQPFFIQ IS+ AR IA +PTNALSRTIP+VVEL
Sbjct: 69  CDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSRTIPMVVEL 128

Query: 122 VLPLPEEEESGSGSDPRVPNGPGRASSRASIEEMERAEIELGICGGDCAICLDEI----- 181
           VLP    E+S  GSD  +  G  RA SRASI+EMER E+   +   DC ICLD+I     
Sbjct: 129 VLPEEAMEDSSYGSDTIMGTGSRRA-SRASIQEMERVEMNGAL--SDCVICLDDIVSVGS 188

Query: 182 ECGAVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME 215
           E  A+ MPC HIYHRNCI KWLQ +N CPLCRFQMPLE E
Sbjct: 189 EIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225

BLAST of MS020087 vs. NCBI nr
Match: KAG7019862.1 (E3 ubiquitin-protein ligase SIRP1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 280.8 bits (717), Expect = 9.9e-72
Identity = 150/220 (68.18%), Postives = 171/220 (77.73%), Query Frame = 0

Query: 2   SAAGLHCEVWQPQEWAESDC--SSSSSEPPEFFIDLYVSSYANQGRETISSLRYKNFRRR 61
           + +GLHCEVWQPQ+++E+ C  SSSSSE PEFFID YVSSYA QG + IS+LRYKNFR+R
Sbjct: 9   AVSGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTLRYKNFRQR 68

Query: 62  RDVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVEL 121
            DV TQDS SWSAISTMLSET+VPYHLQPFFIQ IS+ AR IA +PTNALSRTIP+VVEL
Sbjct: 69  CDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSRTIPMVVEL 128

Query: 122 VLPLPEEEESGSGSDPRVPNGPGRASSRASIEEMERAEIELGICGGDCAICLDEI----- 181
           VLP    E+SG GSD  +  G  RA SR SI+EMER E+  G+   DC ICLD+I     
Sbjct: 129 VLPEEAMEDSGYGSDTIMGTGSRRA-SRTSIQEMERVEMN-GVL-SDCVICLDDIVSVGS 188

Query: 182 ECGAVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME 215
           E  A+ MPC HIYHRNCI KWLQ +N CPLCRFQMPLE E
Sbjct: 189 EIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225

BLAST of MS020087 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 2.2e-10
Identity = 39/96 (40.62%), Postives = 55/96 (57.29%), Query Frame = 0

Query: 119 LVLPLPEEEESGSGSDPRVPNGPGRASSRASIEEMERAEIE--LGICGGDCAICLDEIEC 178
           L+  L + E SG+G   R  N P   +S+++IE + R EI         +CA+C +  E 
Sbjct: 169 LLEQLSQIEASGNGIG-RSGNPP---ASKSAIESLPRVEISDCHTKAEANCAVCTEVFEA 228

Query: 179 GAVG--MPCSHIYHRNCIVKWLQQNNLCPLCRFQMP 211
           G  G  MPC HI+H +CIV WL   N CP+CRF++P
Sbjct: 229 GIEGREMPCKHIFHGDCIVPWLSIRNSCPVCRFELP 260

BLAST of MS020087 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 3.8e-10
Identity = 34/86 (39.53%), Postives = 51/86 (59.30%), Query Frame = 0

Query: 127 EESGSGSDPRVPNGPGRASSRASIEEMERAEIELGICGGDCAICLDEIECG--AVGMPCS 186
           E+  +G+  R P  P   S+  ++  ++ A+  L     +C +C DE E G  A  MPC+
Sbjct: 151 EQLSAGTTRRGP-PPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCN 210

Query: 187 HIYHRNCIVKWLQQNNLCPLCRFQMP 211
           HIYH +CIV WL Q+N CP+CR ++P
Sbjct: 211 HIYHSDCIVPWLVQHNSCPVCRQELP 235

BLAST of MS020087 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 6.5e-10
Identity = 30/101 (29.70%), Postives = 58/101 (57.43%), Query Frame = 0

Query: 114 PIVVELVLPLPEEEESGSGSDPRVPNGPGRASSRASIEEMERAEIELGICGGDCAICLDE 173
           P + +L+  L E + +  G+ P   +      +    ++M ++E+        CA+C+DE
Sbjct: 169 PGLEQLIQQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEM------NQCAVCMDE 228

Query: 174 IECGA--VGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLE 213
            E G+    MPC H++H++C++ WL+ +N CP+CRF++P +
Sbjct: 229 FEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTD 263

BLAST of MS020087 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.1e-09
Identity = 32/96 (33.33%), Postives = 56/96 (58.33%), Query Frame = 0

Query: 119 LVLPLPEEEESGSGSDPRVPNGPGRASSRASIEEMERAEIELGICGGDCAICLDEIECG- 178
           L+  L E + S +G+ P         + + ++E +   +IE  +    C++CLD++E G 
Sbjct: 163 LLQHLAESDPSRNGTPP---------AKKEAVEALPTVKIEEVV---SCSVCLDDLEVGS 222

Query: 179 -AVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLE 213
            A  MPC H +H +CI+ WL+ ++ CP+CRF++P E
Sbjct: 223 QAKQMPCEHKFHSSCILPWLELHSSCPVCRFELPSE 246

BLAST of MS020087 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.5e-09
Identity = 36/96 (37.50%), Postives = 54/96 (56.25%), Query Frame = 0

Query: 119 LVLPLPEEEESGSGSDPRVPNGPGRASSRASIEEMERAEIELGICGGDCAICLD--EIEC 178
           L+  L + E S +G   R  N P   S+  S+  +E ++  +G    +CA+C +  E E 
Sbjct: 153 LLEQLSQIEASATGIG-RSGNPPASKSAIESLPRVEISDCHIG-SEANCAVCTEIFETET 212

Query: 179 GAVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLE 213
            A  MPC H++H +CIV WL   N CP+CRF++P E
Sbjct: 213 EAREMPCKHLFHDDCIVPWLSIRNSCPVCRFELPSE 246

BLAST of MS020087 vs. ExPASy TrEMBL
Match: A0A6J1CAF5 (E3 ubiquitin-protein ligase CIP8-like OS=Momordica charantia OX=3673 GN=LOC111008905 PE=4 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 3.7e-117
Identity = 212/214 (99.07%), Postives = 212/214 (99.07%), Query Frame = 0

Query: 1   MSAAGLHCEVWQPQEWAESDCSSSSSEPPEFFIDLYVSSYANQGRETISSLRYKNFRRRR 60
           MSAAGLHCEVWQPQEWAESDCSSSSSEPPEFFIDLYVSSYANQG ETISSLRYKNFRRRR
Sbjct: 1   MSAAGLHCEVWQPQEWAESDCSSSSSEPPEFFIDLYVSSYANQGGETISSLRYKNFRRRR 60

Query: 61  DVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVELV 120
           DVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVELV
Sbjct: 61  DVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVELV 120

Query: 121 LPLPEEEESGSGSDPRVPNGPGRASSRASIEEMERAEIELGICGGDCAICLDEIECGAVG 180
           LPLPEEEESGSGSDPRVPNGPGRA SRASIEEMERAEIELGICGGDCAICLDEIECGAVG
Sbjct: 121 LPLPEEEESGSGSDPRVPNGPGRA-SRASIEEMERAEIELGICGGDCAICLDEIECGAVG 180

Query: 181 MPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME 215
           MPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME
Sbjct: 181 MPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME 213

BLAST of MS020087 vs. ExPASy TrEMBL
Match: A0A5D3BJ05 (E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G003020 PE=4 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 3.7e-72
Identity = 153/226 (67.70%), Postives = 173/226 (76.55%), Query Frame = 0

Query: 1   MSAA----GLHCEVWQPQEWAESDCSSSSS---EPPEFFIDLYVSSYANQGRETISSLRY 60
           MSAA    GLHCEVWQPQ+++E  CSSSSS   +PPEFFID YVSSYA  G E IS+LRY
Sbjct: 1   MSAAAAISGLHCEVWQPQDFSEPSCSSSSSSQFQPPEFFIDFYVSSYATHGDEVISTLRY 60

Query: 61  KNFRRRRDVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTI 120
           KNFR+R DV TQDS SWSAIS+MLSET+VPYHLQPFFI  IS+RAR IA +P NALSRTI
Sbjct: 61  KNFRQRCDVLTQDSFSWSAISSMLSETNVPYHLQPFFIHQISIRARGIATEPINALSRTI 120

Query: 121 PIVVELVLPLPEEEESGSGSDPRVPNGPGRASSRASIEEMERAEIELGICGGDCAICLDE 180
           P+VVEL+LP    E SG GS+P +  G GRA SRASI+EMER EI+ G+   DC ICLDE
Sbjct: 121 PMVVELMLPEDAMEGSGYGSEPHMGIGSGRA-SRASIQEMERIEID-GVL-SDCVICLDE 180

Query: 181 I-----ECGAVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME 215
           I     E  AV MPC H+YH NCI KWL+ +N CPLCRFQMPLE E
Sbjct: 181 IGSIGCEIDAVRMPCLHVYHLNCIHKWLELSNRCPLCRFQMPLEEE 223

BLAST of MS020087 vs. ExPASy TrEMBL
Match: A0A1S3AU71 (uncharacterized protein LOC103482872 OS=Cucumis melo OX=3656 GN=LOC103482872 PE=4 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 3.7e-72
Identity = 153/226 (67.70%), Postives = 173/226 (76.55%), Query Frame = 0

Query: 1   MSAA----GLHCEVWQPQEWAESDCSSSSS---EPPEFFIDLYVSSYANQGRETISSLRY 60
           MSAA    GLHCEVWQPQ+++E  CSSSSS   +PPEFFID YVSSYA  G E IS+LRY
Sbjct: 1   MSAAAAISGLHCEVWQPQDFSEPSCSSSSSSQFQPPEFFIDFYVSSYATHGDEVISTLRY 60

Query: 61  KNFRRRRDVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTI 120
           KNFR+R DV TQDS SWSAIS+MLSET+VPYHLQPFFI  IS+RAR IA +P NALSRTI
Sbjct: 61  KNFRQRCDVLTQDSFSWSAISSMLSETNVPYHLQPFFIHQISIRARGIATEPINALSRTI 120

Query: 121 PIVVELVLPLPEEEESGSGSDPRVPNGPGRASSRASIEEMERAEIELGICGGDCAICLDE 180
           P+VVEL+LP    E SG GS+P +  G GRA SRASI+EMER EI+ G+   DC ICLDE
Sbjct: 121 PMVVELMLPEDAMEGSGYGSEPHMGIGSGRA-SRASIQEMERIEID-GVL-SDCVICLDE 180

Query: 181 I-----ECGAVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME 215
           I     E  AV MPC H+YH NCI KWL+ +N CPLCRFQMPLE E
Sbjct: 181 IGSIGCEIDAVRMPCLHVYHLNCIHKWLELSNRCPLCRFQMPLEEE 223

BLAST of MS020087 vs. ExPASy TrEMBL
Match: A0A6J1KQS3 (uncharacterized protein LOC111495638 OS=Cucurbita maxima OX=3661 GN=LOC111495638 PE=4 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 4.8e-72
Identity = 149/220 (67.73%), Postives = 170/220 (77.27%), Query Frame = 0

Query: 2   SAAGLHCEVWQPQEWAESDC--SSSSSEPPEFFIDLYVSSYANQGRETISSLRYKNFRRR 61
           + +GLHCEVWQPQ+++E+ C  SSSSSE PEFFID YVSSYA QG + IS+LRYKNFR+R
Sbjct: 9   AVSGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTLRYKNFRQR 68

Query: 62  RDVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVEL 121
            DV TQDS SWSAISTMLSET+VPYHLQPFFIQ IS+ AR IA +PTNALSRTIP+VVEL
Sbjct: 69  CDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSRTIPMVVEL 128

Query: 122 VLPLPEEEESGSGSDPRVPNGPGRASSRASIEEMERAEIELGICGGDCAICLDEI----- 181
           VLP    E+S  GSD  +  G  RA SRASI+EMER E+   +   DC ICLD+I     
Sbjct: 129 VLPEEAMEDSSYGSDTIMGTGSRRA-SRASIQEMERVEMNGAL--SDCVICLDDIVSVGS 188

Query: 182 ECGAVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME 215
           E  A+ MPC HIYHRNCI KWLQ +N CPLCRFQMPLE E
Sbjct: 189 EIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225

BLAST of MS020087 vs. ExPASy TrEMBL
Match: A0A0A0LR28 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G045460 PE=4 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 8.1e-72
Identity = 148/220 (67.27%), Postives = 169/220 (76.82%), Query Frame = 0

Query: 4   AGLHCEVWQPQEWAESDCSSSSS----EPPEFFIDLYVSSYANQGRETISSLRYKNFRRR 63
           +GLHCEVWQPQ+++E  CSSSSS    +PPEFFID YVSSYA  G E IS+LRYKNFR+R
Sbjct: 7   SGLHCEVWQPQDFSEPSCSSSSSSSQFQPPEFFIDFYVSSYATHGDEVISTLRYKNFRQR 66

Query: 64  RDVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVEL 123
            DV TQDS SWSAIS+MLSET+VPYHLQPFFI  IS RAR IA +P NALSRTIP+VVEL
Sbjct: 67  CDVLTQDSFSWSAISSMLSETNVPYHLQPFFIHQISTRARGIATEPINALSRTIPMVVEL 126

Query: 124 VLPLPEEEESGSGSDPRVPNGPGRASSRASIEEMERAEIELGICGGDCAICLDEI----- 183
           +LP    E+SG GS+P +  G GRA SRASI+EMER EI+ G+   DC ICLDEI     
Sbjct: 127 ILPEDAMEDSGYGSEPHMGIGSGRA-SRASIQEMERIEID-GVL-SDCVICLDEIGSIGC 186

Query: 184 ECGAVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME 215
           E   V MPC H+YH NCI KWL+ +N CPLCRFQMPLE E
Sbjct: 187 EIDVVQMPCLHVYHLNCIHKWLELSNRCPLCRFQMPLEEE 223

BLAST of MS020087 vs. TAIR 10
Match: AT3G46620.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 67.4 bits (163), Expect = 1.6e-11
Identity = 39/96 (40.62%), Postives = 55/96 (57.29%), Query Frame = 0

Query: 119 LVLPLPEEEESGSGSDPRVPNGPGRASSRASIEEMERAEIE--LGICGGDCAICLDEIEC 178
           L+  L + E SG+G   R  N P   +S+++IE + R EI         +CA+C +  E 
Sbjct: 169 LLEQLSQIEASGNGIG-RSGNPP---ASKSAIESLPRVEISDCHTKAEANCAVCTEVFEA 228

Query: 179 GAVG--MPCSHIYHRNCIVKWLQQNNLCPLCRFQMP 211
           G  G  MPC HI+H +CIV WL   N CP+CRF++P
Sbjct: 229 GIEGREMPCKHIFHGDCIVPWLSIRNSCPVCRFELP 260

BLAST of MS020087 vs. TAIR 10
Match: AT1G14200.1 (RING/U-box superfamily protein )

HSP 1 Score: 66.6 bits (161), Expect = 2.7e-11
Identity = 47/151 (31.13%), Postives = 75/151 (49.67%), Query Frame = 0

Query: 90  FIQHISLRARRIAAQPTNALSRTIPIVVELVLPLPEEEE--------------------- 149
           F++  S+  R I  +  + LSR +P++V L     ++++                     
Sbjct: 7   FVEFSSMFERIIQGR-GDGLSRFLPVIVALAAREDDDDQGSTDQTTRRGDPLSPRFVMIG 66

Query: 150 SGSGSDPRVPNG--PGRASS-RASIEEMERAEI--ELGICGGDCAICLDEIECG--AVGM 209
           S SG D    +G   GR+ + ++ +E M R  I  +    GG CAICLDE   G  A  M
Sbjct: 67  SRSGLDDFFSDGGKQGRSPALKSEVENMPRVVIGEDKEKYGGSCAICLDEWSKGDVAAEM 126

Query: 210 PCSHIYHRNCIVKWLQQNNLCPLCRFQMPLE 213
           PC H +H  C+ +WL ++  CP+CR++MP+E
Sbjct: 127 PCKHKFHSKCVEEWLGRHATCPMCRYEMPVE 156

BLAST of MS020087 vs. TAIR 10
Match: AT2G40830.2 (RING-H2 finger C1A )

HSP 1 Score: 66.6 bits (161), Expect = 2.7e-11
Identity = 34/86 (39.53%), Postives = 51/86 (59.30%), Query Frame = 0

Query: 127 EESGSGSDPRVPNGPGRASSRASIEEMERAEIELGICGGDCAICLDEIECG--AVGMPCS 186
           E+  +G+  R P  P   S+  ++  ++ A+  L     +C +C DE E G  A  MPC+
Sbjct: 151 EQLSAGTTRRGP-PPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCN 210

Query: 187 HIYHRNCIVKWLQQNNLCPLCRFQMP 211
           HIYH +CIV WL Q+N CP+CR ++P
Sbjct: 211 HIYHSDCIVPWLVQHNSCPVCRQELP 235

BLAST of MS020087 vs. TAIR 10
Match: AT2G40830.1 (RING-H2 finger C1A )

HSP 1 Score: 66.6 bits (161), Expect = 2.7e-11
Identity = 34/86 (39.53%), Postives = 51/86 (59.30%), Query Frame = 0

Query: 127 EESGSGSDPRVPNGPGRASSRASIEEMERAEIELGICGGDCAICLDEIECG--AVGMPCS 186
           E+  +G+  R P  P   S+  ++  ++ A+  L     +C +C DE E G  A  MPC+
Sbjct: 151 EQLSAGTTRRGP-PPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCN 210

Query: 187 HIYHRNCIVKWLQQNNLCPLCRFQMP 211
           HIYH +CIV WL Q+N CP+CR ++P
Sbjct: 211 HIYHSDCIVPWLVQHNSCPVCRQELP 235

BLAST of MS020087 vs. TAIR 10
Match: AT2G40830.3 (RING-H2 finger C1A )

HSP 1 Score: 66.6 bits (161), Expect = 2.7e-11
Identity = 34/86 (39.53%), Postives = 51/86 (59.30%), Query Frame = 0

Query: 127 EESGSGSDPRVPNGPGRASSRASIEEMERAEIELGICGGDCAICLDEIECG--AVGMPCS 186
           E+  +G+  R P  P   S+  ++  ++ A+  L     +C +C DE E G  A  MPC+
Sbjct: 151 EQLSAGTTRRGP-PPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCN 210

Query: 187 HIYHRNCIVKWLQQNNLCPLCRFQMP 211
           HIYH +CIV WL Q+N CP+CR ++P
Sbjct: 211 HIYHSDCIVPWLVQHNSCPVCRQELP 235

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022137468.17.7e-11799.07E3 ubiquitin-protein ligase CIP8-like [Momordica charantia][more]
KAG6584266.13.4e-7268.64E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_008437461.17.6e-7267.70PREDICTED: uncharacterized protein LOC103482872 [Cucumis melo] >TYJ99097.1 E3 ub... [more]
XP_023001523.19.9e-7267.73uncharacterized protein LOC111495638 [Cucurbita maxima][more]
KAG7019862.19.9e-7268.18E3 ubiquitin-protein ligase SIRP1, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
Q9SNB62.2e-1040.63E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ... [more]
O221973.8e-1039.53Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Q8LPN76.5e-1029.70E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19... [more]
Q6AVN21.1e-0933.33E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI... [more]
Q940T51.5e-0937.50E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1CAF53.7e-11799.07E3 ubiquitin-protein ligase CIP8-like OS=Momordica charantia OX=3673 GN=LOC11100... [more]
A0A5D3BJ053.7e-7267.70E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3AU713.7e-7267.70uncharacterized protein LOC103482872 OS=Cucumis melo OX=3656 GN=LOC103482872 PE=... [more]
A0A6J1KQS34.8e-7267.73uncharacterized protein LOC111495638 OS=Cucurbita maxima OX=3661 GN=LOC111495638... [more]
A0A0A0LR288.1e-7267.27RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G045460 P... [more]
Match NameE-valueIdentityDescription
AT3G46620.11.6e-1140.63zinc finger (C3HC4-type RING finger) family protein [more]
AT1G14200.12.7e-1131.13RING/U-box superfamily protein [more]
AT2G40830.22.7e-1139.53RING-H2 finger C1A [more]
AT2G40830.12.7e-1139.53RING-H2 finger C1A [more]
AT2G40830.32.7e-1139.53RING-H2 finger C1A [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 167..205
e-value: 1.4E-6
score: 37.9
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 166..206
e-value: 2.5E-11
score: 43.7
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 167..206
score: 12.54829
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 140..211
e-value: 3.7E-16
score: 60.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 126..149
NoneNo IPR availablePANTHERPTHR15710E3 UBIQUITIN-PROTEIN LIGASE PRAJAcoord: 6..210
NoneNo IPR availablePANTHERPTHR15710:SF138FINGER PROTEIN, PUTATIVE-RELATEDcoord: 6..210
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 166..210

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS020087.1MS020087.1mRNA