MS020082 (gene) Bitter gourd (TR) v1

BLAST of MS020082 vs. NCBI nr
Match: XP_022137625.1 (pectate lyase-like isoform X1 [Momordica charantia])

HSP 1 Score: 867.5 bits (2240), Expect = 5.1e-248
Identity = 408/409 (99.76%), Postives = 409/409 (100.00%), Query Frame = 0

Query: 32  DFDEVWQKRAQEAKKAAIEAYQPHPEEVTSNFNQQVHKSLDGANNTRRHLKHYSGGPCLA 91
           +FDEVWQKRAQEAKKAAIEAYQPHPEEVTSNFNQQVHKSLDGANNTRRHLKHYSGGPCLA
Sbjct: 50  NFDEVWQKRAQEAKKAAIEAYQPHPEEVTSNFNQQVHKSLDGANNTRRHLKHYSGGPCLA 109

Query: 92  TNPIDRCWRCDKHWAKNRKKLAECALGFGRGTTGGKDGRYYVVRDSSDNDLLNPKPGTLR 151
           TNPIDRCWRCDKHWAKNRKKLAECALGFGRGTTGGKDGRYYVVRDSSDNDLLNPKPGTLR
Sbjct: 110 TNPIDRCWRCDKHWAKNRKKLAECALGFGRGTTGGKDGRYYVVRDSSDNDLLNPKPGTLR 169

Query: 152 YGVIQEKPLWIVFAHDMVIRLSEELIITDNKTIDGRGANVHIANGGQITLQFVKNIIISN 211
           YGVIQEKPLWIVFAHDMVIRLSEELIITDNKTIDGRGANVHIANGGQITLQFVKNIIISN
Sbjct: 170 YGVIQEKPLWIVFAHDMVIRLSEELIITDNKTIDGRGANVHIANGGQITLQFVKNIIISN 229

Query: 212 LHIHDIKNGHGGLIRDSVSHYGLRTPSDGDGISMFGATKIWIDHVSMSNCQDGLIDAVMA 271
           LHIHDIKNGHGGLIRDSVSHYGLRTPSDGDGISMFGATKIWIDHVSMSNCQDGLIDAVMA
Sbjct: 230 LHIHDIKNGHGGLIRDSVSHYGLRTPSDGDGISMFGATKIWIDHVSMSNCQDGLIDAVMA 289

Query: 272 STGITISNCHFTRHNDVMLLGASDSYSNDQIMQVTLAFNHFGKGLVQRMPRCRWGFVHVV 331
           STGITISNCHFTRHNDVMLLGASDSYSNDQIMQVTLAFNHFGKGLVQRMPRCRWGFVHVV
Sbjct: 290 STGITISNCHFTRHNDVMLLGASDSYSNDQIMQVTLAFNHFGKGLVQRMPRCRWGFVHVV 349

Query: 332 NNDYTHWLMYAIGGSHNPTIISQGNRFIAPPDPNCKEVTKRAYASESEWKSWNWRSEGDL 391
           NNDYTHWLMYAIGGSHNPTIISQGNRFIAPPDPNCKEVTKRAYASESEWKSWNWRSEGDL
Sbjct: 350 NNDYTHWLMYAIGGSHNPTIISQGNRFIAPPDPNCKEVTKRAYASESEWKSWNWRSEGDL 409

Query: 392 MMNGAFFIESGSPIRKYSKKDVITAKPGSFVTRLTRFSGPLKCRNNRPC 441
           MMNGAFFIESGSPIRKYSKKDVITAKPGSFVTRLTRFSGPLKCRNNRPC
Sbjct: 410 MMNGAFFIESGSPIRKYSKKDVITAKPGSFVTRLTRFSGPLKCRNNRPC 458

BLAST of MS020082 vs. NCBI nr
Match: XP_022137626.1 (pectate lyase-like isoform X2 [Momordica charantia])

HSP 1 Score: 852.8 bits (2202), Expect = 1.3e-243
Identity = 402/403 (99.75%), Postives = 403/403 (100.00%), Query Frame = 0

Query: 38  QKRAQEAKKAAIEAYQPHPEEVTSNFNQQVHKSLDGANNTRRHLKHYSGGPCLATNPIDR 97
           +KRAQEAKKAAIEAYQPHPEEVTSNFNQQVHKSLDGANNTRRHLKHYSGGPCLATNPIDR
Sbjct: 15  KKRAQEAKKAAIEAYQPHPEEVTSNFNQQVHKSLDGANNTRRHLKHYSGGPCLATNPIDR 74

Query: 98  CWRCDKHWAKNRKKLAECALGFGRGTTGGKDGRYYVVRDSSDNDLLNPKPGTLRYGVIQE 157
           CWRCDKHWAKNRKKLAECALGFGRGTTGGKDGRYYVVRDSSDNDLLNPKPGTLRYGVIQE
Sbjct: 75  CWRCDKHWAKNRKKLAECALGFGRGTTGGKDGRYYVVRDSSDNDLLNPKPGTLRYGVIQE 134

Query: 158 KPLWIVFAHDMVIRLSEELIITDNKTIDGRGANVHIANGGQITLQFVKNIIISNLHIHDI 217
           KPLWIVFAHDMVIRLSEELIITDNKTIDGRGANVHIANGGQITLQFVKNIIISNLHIHDI
Sbjct: 135 KPLWIVFAHDMVIRLSEELIITDNKTIDGRGANVHIANGGQITLQFVKNIIISNLHIHDI 194

Query: 218 KNGHGGLIRDSVSHYGLRTPSDGDGISMFGATKIWIDHVSMSNCQDGLIDAVMASTGITI 277
           KNGHGGLIRDSVSHYGLRTPSDGDGISMFGATKIWIDHVSMSNCQDGLIDAVMASTGITI
Sbjct: 195 KNGHGGLIRDSVSHYGLRTPSDGDGISMFGATKIWIDHVSMSNCQDGLIDAVMASTGITI 254

Query: 278 SNCHFTRHNDVMLLGASDSYSNDQIMQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTH 337
           SNCHFTRHNDVMLLGASDSYSNDQIMQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTH
Sbjct: 255 SNCHFTRHNDVMLLGASDSYSNDQIMQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTH 314

Query: 338 WLMYAIGGSHNPTIISQGNRFIAPPDPNCKEVTKRAYASESEWKSWNWRSEGDLMMNGAF 397
           WLMYAIGGSHNPTIISQGNRFIAPPDPNCKEVTKRAYASESEWKSWNWRSEGDLMMNGAF
Sbjct: 315 WLMYAIGGSHNPTIISQGNRFIAPPDPNCKEVTKRAYASESEWKSWNWRSEGDLMMNGAF 374

Query: 398 FIESGSPIRKYSKKDVITAKPGSFVTRLTRFSGPLKCRNNRPC 441
           FIESGSPIRKYSKKDVITAKPGSFVTRLTRFSGPLKCRNNRPC
Sbjct: 375 FIESGSPIRKYSKKDVITAKPGSFVTRLTRFSGPLKCRNNRPC 417

BLAST of MS020082 vs. NCBI nr
Match: XP_038894610.1 (pectate lyase-like isoform X2 [Benincasa hispida])

HSP 1 Score: 847.0 bits (2187), Expect = 7.1e-242
Identity = 394/440 (89.55%), Postives = 426/440 (96.82%), Query Frame = 0

Query: 1   MRETETTIIGLKLLMILSCVMIFPTLKANIADFDEVWQKRAQEAKKAAIEAYQPHPEEVT 60
           MRETETTI+G+KLLMI   VMIFPTLKANIADFDEVWQKRA+EAKKA+ EAYQPHPEEVT
Sbjct: 1   MRETETTIVGIKLLMIFGFVMIFPTLKANIADFDEVWQKRAEEAKKASFEAYQPHPEEVT 60

Query: 61  SNFNQQVHKSLDGANNTRRHLKHYSGGPCLATNPIDRCWRCDKHWAKNRKKLAECALGFG 120
           SNFN+QVHKSLDGANNTRRHL+ Y+ GPCLATNPIDRCWRCDKHWAKNRKKLA+CALGFG
Sbjct: 61  SNFNKQVHKSLDGANNTRRHLRKYN-GPCLATNPIDRCWRCDKHWAKNRKKLADCALGFG 120

Query: 121 RGTTGGKDGRYYVVRDSSDNDLLNPKPGTLRYGVIQEKPLWIVFAHDMVIRLSEELIITD 180
           RGTTGGK+GR YVVRDSSDNDLLNPKPGTLRYGVIQ++PLWI+FAHDMVIRLSEELI+TD
Sbjct: 121 RGTTGGKNGRIYVVRDSSDNDLLNPKPGTLRYGVIQDRPLWIIFAHDMVIRLSEELIMTD 180

Query: 181 NKTIDGRGANVHIANGGQITLQFVKNIIISNLHIHDIKNGHGGLIRDSVSHYGLRTPSDG 240
           NKT+DGRGANVHIANGGQ+TLQFVKNIII NLHIHDIK G+GG+IRDSV+HYG RTPSDG
Sbjct: 181 NKTLDGRGANVHIANGGQLTLQFVKNIIIHNLHIHDIKAGNGGIIRDSVNHYGFRTPSDG 240

Query: 241 DGISMFGATKIWIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVMLLGASDSYSND 300
           DGISMFGA+K+WIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDV+LLGASDSYSND
Sbjct: 241 DGISMFGASKVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVILLGASDSYSND 300

Query: 301 QIMQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIA 360
           QIMQVTLAFNHFGKGLVQRMPRCRWGF+HVVNNDYTHWLMYAIGGSHNPTIISQGNRFIA
Sbjct: 301 QIMQVTLAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIA 360

Query: 361 PPDPNCKEVTKRAYASESEWKSWNWRSEGDLMMNGAFFIESGSPIRKYSKKDVITAKPGS 420
           PP+PN KEVTKRAYASESEW+SWNWRSEGDLM+NGAFFI+SG+PIR+YSKKDVI +KPG+
Sbjct: 361 PPNPNSKEVTKRAYASESEWRSWNWRSEGDLMLNGAFFIQSGNPIRRYSKKDVIHSKPGT 420

Query: 421 FVTRLTRFSGPLKCRNNRPC 441
           FVTRLTRF+GPLKC  N+PC
Sbjct: 421 FVTRLTRFAGPLKCIKNQPC 439

BLAST of MS020082 vs. NCBI nr
Match: TYJ99102.1 (pectate lyase-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 820.8 bits (2119), Expect = 5.4e-234
Identity = 380/440 (86.36%), Postives = 420/440 (95.45%), Query Frame = 0

Query: 1   MRETETTIIGLKLLMILSCVMIFPTLKANIADFDEVWQKRAQEAKKAAIEAYQPHPEEVT 60
           MRET+TTI+G+KLLMI   VMIFPTLKANIADFDEVWQKRA EAKKA++EAYQPHPEEVT
Sbjct: 1   MRETKTTIVGIKLLMIFGFVMIFPTLKANIADFDEVWQKRAIEAKKASLEAYQPHPEEVT 60

Query: 61  SNFNQQVHKSLDGANNTRRHLKHYSGGPCLATNPIDRCWRCDKHWAKNRKKLAECALGFG 120
           +NFN+QVH+SLDGANNTRRHL+ Y+ GPCLATNPIDRCWRCD++WA+NRKKLA+CALGFG
Sbjct: 61  NNFNKQVHRSLDGANNTRRHLRKYT-GPCLATNPIDRCWRCDRNWARNRKKLADCALGFG 120

Query: 121 RGTTGGKDGRYYVVRDSSDNDLLNPKPGTLRYGVIQEKPLWIVFAHDMVIRLSEELIITD 180
           RGTTGGKDG+ YVVRDSSDNDL+NPKPGTLR+ VIQE+PLWI+FAHDMVIRLSEELI+TD
Sbjct: 121 RGTTGGKDGKIYVVRDSSDNDLVNPKPGTLRHAVIQERPLWIIFAHDMVIRLSEELIMTD 180

Query: 181 NKTIDGRGANVHIANGGQITLQFVKNIIISNLHIHDIKNGHGGLIRDSVSHYGLRTPSDG 240
           NKT+DGRGANVHIANGGQITLQFV+NIII NLHIHDIK G+GG IRDSVSHYG RT SDG
Sbjct: 181 NKTLDGRGANVHIANGGQITLQFVRNIIIHNLHIHDIKAGNGGFIRDSVSHYGYRTQSDG 240

Query: 241 DGISMFGATKIWIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVMLLGASDSYSND 300
           DGISMFG++++WIDHVSMSNCQDGLIDAVMAST ITISNCHFT HNDV+LLGASDSYSND
Sbjct: 241 DGISMFGSSRVWIDHVSMSNCQDGLIDAVMASTAITISNCHFTHHNDVILLGASDSYSND 300

Query: 301 QIMQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIA 360
           +IMQVTLAFNHFGKGLVQRMPRCRWGF+HVVNNDYTHWLMYAIGGSHNPTIISQGNRFIA
Sbjct: 301 KIMQVTLAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIA 360

Query: 361 PPDPNCKEVTKRAYASESEWKSWNWRSEGDLMMNGAFFIESGSPIRKYSKKDVITAKPGS 420
           PP+PN KEVTKR YASE EW+SWNWRSEGDLMMNGAFFI+SG+PIR+YSKKDVI +KPG+
Sbjct: 361 PPNPNSKEVTKRVYASEREWRSWNWRSEGDLMMNGAFFIQSGNPIRRYSKKDVIHSKPGT 420

Query: 421 FVTRLTRFSGPLKCRNNRPC 441
           FVTRLTRF+GPLKC+ N+PC
Sbjct: 421 FVTRLTRFAGPLKCKMNQPC 439

BLAST of MS020082 vs. NCBI nr
Match: XP_004152537.2 (pectate lyase isoform X1 [Cucumis sativus] >KGN64253.1 hypothetical protein Csa_013613 [Cucumis sativus])

HSP 1 Score: 813.9 bits (2101), Expect = 6.7e-232
Identity = 375/440 (85.23%), Postives = 417/440 (94.77%), Query Frame = 0

Query: 1   MRETETTIIGLKLLMILSCVMIFPTLKANIADFDEVWQKRAQEAKKAAIEAYQPHPEEVT 60
           MRET+TTI+G+KLLMI   VMIFPTLKANIADFDEVWQKRA EA+KA+ EAY+PHPEE T
Sbjct: 1   MRETKTTIVGIKLLMIFGFVMIFPTLKANIADFDEVWQKRAIEAQKASFEAYEPHPEEET 60

Query: 61  SNFNQQVHKSLDGANNTRRHLKHYSGGPCLATNPIDRCWRCDKHWAKNRKKLAECALGFG 120
           +NFN+QVH+SLDG NNTRRHL+ Y+ GPCLATNPIDRCWRCD++WA+NRKKLA+CALGFG
Sbjct: 61  NNFNKQVHRSLDGGNNTRRHLRKYT-GPCLATNPIDRCWRCDRNWARNRKKLADCALGFG 120

Query: 121 RGTTGGKDGRYYVVRDSSDNDLLNPKPGTLRYGVIQEKPLWIVFAHDMVIRLSEELIITD 180
           R TTGGKDG+ YVVRDSSDNDL+NPKPGTLR+ VIQE+PLWI+FAHDMVIRLSEELI+TD
Sbjct: 121 RRTTGGKDGKIYVVRDSSDNDLVNPKPGTLRHAVIQERPLWIIFAHDMVIRLSEELIVTD 180

Query: 181 NKTIDGRGANVHIANGGQITLQFVKNIIISNLHIHDIKNGHGGLIRDSVSHYGLRTPSDG 240
           +KT+DGRGANVHIANGGQITLQFVKNIII NLHIHDIK G+GG+IRDSVSHYG RT SDG
Sbjct: 181 DKTLDGRGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGMIRDSVSHYGFRTRSDG 240

Query: 241 DGISMFGATKIWIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVMLLGASDSYSND 300
           DGISMFGA+++WIDHVSMSNCQDGLIDAVMAST ITISNCHFT HNDV+LLGAS+ YSND
Sbjct: 241 DGISMFGASRVWIDHVSMSNCQDGLIDAVMASTAITISNCHFTHHNDVILLGASNGYSND 300

Query: 301 QIMQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIA 360
           QIMQVTLAFNHFGKGLVQRMPRCRWGF+HVVNNDYTHWLMYAIGGSHNPTIISQGNRFIA
Sbjct: 301 QIMQVTLAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIA 360

Query: 361 PPDPNCKEVTKRAYASESEWKSWNWRSEGDLMMNGAFFIESGSPIRKYSKKDVITAKPGS 420
           PP+PNCKEVTKR YA ESEW+SWNWRSEGDLMMNGAFFI+SG+PI++YSKKDVI +KPG+
Sbjct: 361 PPNPNCKEVTKRVYAPESEWRSWNWRSEGDLMMNGAFFIQSGNPIKRYSKKDVIHSKPGT 420

Query: 421 FVTRLTRFSGPLKCRNNRPC 441
           FVTRLTRF+GPLKC+ N+PC
Sbjct: 421 FVTRLTRFAGPLKCKKNQPC 439

BLAST of MS020082 vs. ExPASy Swiss-Prot
Match: P40973 (Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 6.4e-169
Identity = 275/414 (66.43%), Postives = 332/414 (80.19%), Query Frame = 0

Query: 28  ANIADFDEVWQKRAQEAKKAAIEAYQPHPEEVTSNFNQQVHKSLDGANNTRRHLKHYSGG 87
           ANIA+FDE WQK+++ A+  A +AY PHPEEVT++FN+ VH S +G N+TRR+L+    G
Sbjct: 22  ANIAEFDEYWQKKSKVAQAKAKKAYTPHPEEVTNHFNKAVHSSFEG-NSTRRNLRTNKLG 81

Query: 88  PCLATNPIDRCWRCDKHWAKNRKKLAECALGFGRGTTGGKDGRYYVVRDSSDNDLLNPKP 147
            CLATNPIDRCWRC K+W+ NRK L +C  GFGR TTGG  G  YVV D SD+ L +PK 
Sbjct: 82  QCLATNPIDRCWRCKKNWSANRKDLVKCVKGFGRKTTGGAAGEIYVVTDPSDDSLTDPKF 141

Query: 148 GTLRYGVIQEKPLWIVFAHDMVIRLSEELIITDNKTIDGRGANVHIANGGQITLQFVKNI 207
           GTLR+GVIQ++PLWI+F   MVIRL +ELII ++KTIDGRGANV IA G Q+T+QFV N+
Sbjct: 142 GTLRWGVIQDRPLWIIFGKSMVIRLKQELIINNDKTIDGRGANVQIAGGAQLTVQFVHNV 201

Query: 208 IISNLHIHDIKNGHGGLIRDSVSHYGLRTPSDGDGISMFGATKIWIDHVSMSNCQDGLID 267
           II  +HIHDIK G GGLIRDS  H G+RT SDGDGIS+ G++ IWIDHVS++ C DGLID
Sbjct: 202 IIHGIHIHDIKPGEGGLIRDSEKHSGIRTRSDGDGISIIGSSNIWIDHVSLARCSDGLID 261

Query: 268 AVMASTGITISNCHFTRHNDVMLLGASDSYSNDQIMQVTLAFNHFGKGLVQRMPRCRWGF 327
            ++ ST ITISNCH T H+DVMLLGASD+Y+ D+IMQVT+AFNHFG+GLVQRMPRCR+GF
Sbjct: 262 VILGSTAITISNCHLTEHDDVMLLGASDTYTQDEIMQVTVAFNHFGRGLVQRMPRCRYGF 321

Query: 328 VHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPDPNCKEVTKRAYASESEWKSWNWRS 387
           VHVVNNDYTHW+MYA+GGS +PTIISQGNR+IAP     KEVTKR YA  +EW  W W+S
Sbjct: 322 VHVVNNDYTHWIMYAVGGSQHPTIISQGNRYIAPHIEAAKEVTKRDYAEPAEWSKWTWKS 381

Query: 388 EGDLMMNGAFFIESGSPI-RKYSKKDVITAKPGSFVTRLTRFSGPLKCRNNRPC 441
           +GDL ++GAFF+ESG P   KYSKKD+I AKPG+FV RLTRFSG L C+ N  C
Sbjct: 382 QGDLFVSGAFFVESGGPFENKYSKKDLIKAKPGTFVQRLTRFSGALNCKENMEC 434

BLAST of MS020082 vs. ExPASy Swiss-Prot
Match: P15722 (Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 1.1e-157
Identity = 271/441 (61.45%), Postives = 329/441 (74.60%), Query Frame = 0

Query: 13  LLMILSCVMIFPTLKANIADFDEVWQKRAQEAKKAAIEAYQPHPEEVTSNFNQQVH---- 72
           L + ++   I P+L A+I  +DEVW++RA+EAK+ A   Y+PHPE VT  FNQ++     
Sbjct: 11  LFLCITFATIIPSLMAHIGHYDEVWRRRAEEAKEYARNIYEPHPENVTLAFNQKLRDTMK 70

Query: 73  --KSLDGA--NNTRRHL--KHYSGGPCLATNPIDRCWRCDKHWAKNRKKLAECALGFGRG 132
             K + G   N+TRR L  K Y+ GPC+ TNPID+CWRCD +WA NRKKLA+CA+GFG  
Sbjct: 71  ELKKVKGTHNNSTRRGLGTKKYT-GPCMVTNPIDKCWRCDPNWADNRKKLADCAMGFGSK 130

Query: 133 TTGGKDGRYYVVRDSSDNDLLNPKPGTLRYGVIQEKPLWIVFAHDMVIRLSEELIITDNK 192
             GGKDG +YVV D+SD D  +PKPGTLR+ VIQ++PLWI+F   M IRL +E+I+  +K
Sbjct: 131 AIGGKDGEFYVVTDNSD-DYNDPKPGTLRHAVIQKEPLWIIFKRGMNIRLHQEMIMQSDK 190

Query: 193 TIDGRGANVHIANGGQITLQFVKNIIISNLHIHDIKNGHGGLIRDSVSHYGLRTPSDGDG 252
           TID RG NVHI  G  ITLQ++KN+II  LHIHDI  G+GG++RD+V H G+RT SDGDG
Sbjct: 191 TIDARGVNVHITKGAGITLQYIKNVIIHGLHIHDIVEGNGGMVRDAVDHIGIRTKSDGDG 250

Query: 253 ISMFGATKIWIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVMLLGASDSYSNDQI 312
           IS+FGA+ IWIDHVSM  C DGLIDAV  STGITISN HFT HN+VML GASDS S DQ+
Sbjct: 251 ISIFGASYIWIDHVSMQRCYDGLIDAVEGSTGITISNGHFTDHNEVMLFGASDSSSIDQV 310

Query: 313 MQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPP 372
           MQ+TLAFNHFGK L+QRMPRCRWG++HVVNNDYTHW MYAIGGS +PTII QGNRFIAPP
Sbjct: 311 MQITLAFNHFGKRLIQRMPRCRWGYIHVVNNDYTHWNMYAIGGSMHPTIIHQGNRFIAPP 370

Query: 373 DPNCKEVTKRAYASESEWKSWNWRSEGDLMMNGAFFIESGSPIRKYSKKDV---ITAKPG 432
           D   K+VTKR Y  ES W  W WRSEG+L MNGA+F ESG P      KD+   I+A P 
Sbjct: 371 DIFKKQVTKREYNPESVWMQWTWRSEGNLFMNGAYFTESGDPEWSSKHKDLYDGISAAPA 430

Query: 433 SFVTRLTRFSGPLKCRNNRPC 441
             VT +TRF+G L C+  +PC
Sbjct: 431 EDVTWMTRFAGVLGCKPGKPC 449

BLAST of MS020082 vs. ExPASy Swiss-Prot
Match: Q9M9S2 (Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 1.6e-151
Identity = 253/438 (57.76%), Postives = 316/438 (72.15%), Query Frame = 0

Query: 21  MIFPTLKANIADFDEVWQKRAQEAKKAAIEAYQPHPEEVTSNFNQQVHKSLDGA---NNT 80
           ++ P ++ N+A FD  W +R  +A K  I +Y PHP  VT++FN  V+ ++D +   N+T
Sbjct: 22  IVAPQVRGNVAVFDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNIAVDASESRNDT 81

Query: 81  RRHL--------KHYSGGPCLATNPIDRCWRCDKHWAKNRKKLAECALGFGRGTTGGKDG 140
           RR L         H S G CLA NPID CWRCD++WA NRKKLA+C LGFGR TTGGKDG
Sbjct: 82  RRELTQVRSGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFGRRTTGGKDG 141

Query: 141 RYYVVRDSSDNDLLNPKPGTLRYGVIQEKPLWIVFAHDMVIRLSEELIITDNKTIDGRGA 200
             YVV+D+SDNDL+NPKPGTLR+ V ++ PLWI+FA  M+I+L +EL+IT +KTIDGRGA
Sbjct: 142 PIYVVKDASDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITSDKTIDGRGA 201

Query: 201 NVHIANGGQITLQFVKNIIISNLHIHDIKNGHGGLIRDSVSHYGLRTPSDGDGISMFGAT 260
            V+I  G  +TLQFV N+II N+++  I  G+GGLIRDS +H GLRT SDGDGIS+FGAT
Sbjct: 202 RVYIMEGAGLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDGDGISLFGAT 261

Query: 261 KIWIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVMLLGASDSYSNDQIMQVTLAF 320
            IWIDHVSM+ C DG+IDA+  ST +TISN HFT H +VML GA D +  D+ MQ+T+AF
Sbjct: 262 NIWIDHVSMTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVIDKKMQITVAF 321

Query: 321 NHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPDPNCKEV 380
           NHFGK L QRMPRCR+G +HVVNNDYTHW MYAIGG+ NPTIISQGNRFIAPP+   K++
Sbjct: 322 NHFGKRLEQRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPNEEAKQI 381

Query: 381 TKRAYASESEWKSWNWRSEGDLMMNGAFFIESG-------SPIRKYSKKDVITAKPGSFV 440
           TKR Y    EWKSWNW+SEGD  +NGA+F++SG        P      K  I  KPG+ V
Sbjct: 382 TKREYTPYGEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFTIRPKPGTMV 441

BLAST of MS020082 vs. ExPASy Swiss-Prot
Match: Q9LFP5 (Probable pectate lyase 19 OS=Arabidopsis thaliana OX=3702 GN=At5g15110 PE=2 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 1.3e-150
Identity = 256/470 (54.47%), Postives = 334/470 (71.06%), Query Frame = 0

Query: 8   IIGLKLLMILSCV-MIFPTLKANIADFDEVWQKRAQEAKKAAIEAYQPHPEEVTSNFNQQ 67
           ++ L  LM   C+ ++ PT++ NI++ DE W +RA EA++  ++AY   P E+  +F+++
Sbjct: 3   MVRLSKLMFTFCIAVLIPTIRGNISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHER 62

Query: 68  VHKSL--------DG------------------ANNTRRHLKHYS-------GGPCLATN 127
            + +         DG                   N+TRR L+           GPC A+N
Sbjct: 63  HYDNSTDVTTPEEDGDAKPEEEEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASN 122

Query: 128 PIDRCWRCDKHWAKNRKKLAECALGFGRGTTGGKDGRYYVVRDSSDNDLLNPKPGTLRYG 187
           PID+CWRC   WAK RKKL  C  GFG  TTGGK GR YVV  + D D++NPKPGTLR+ 
Sbjct: 123 PIDKCWRCRSDWAKRRKKLTRCVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHA 182

Query: 188 VIQEKPLWIVFAHDMVIRLSEELIITDNKTIDGRGANVHIANGGQITLQFVKNIIISNLH 247
           VIQ++PLWI+F +DM IRL++EL+I  +KTID RGANVH+A+G  IT+QFVKN+II  LH
Sbjct: 183 VIQKEPLWIIFKNDMSIRLNQELLINSHKTIDARGANVHVAHGAGITMQFVKNVIIHGLH 242

Query: 248 IHDIKNGHGGLIRDSVSHYGLRTPSDGDGISMFGATKIWIDHVSMSNCQDGLIDAVMAST 307
           IH I    GG+IRDSV H+G+RT +DGDG+S++G++ IW+DH+SMS CQDGLIDA++ ST
Sbjct: 243 IHHISESSGGMIRDSVDHFGMRTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGST 302

Query: 308 GITISNCHFTRHNDVMLLGASDSYSNDQIMQVTLAFNHFGKGLVQRMPRCRWGFVHVVNN 367
           GITISN HFT HNDVMLLGA ++   D+ MQVT+A+NHFGKGLVQRMPR RWGFVHVVNN
Sbjct: 303 GITISNSHFTHHNDVMLLGAQNTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNN 362

Query: 368 DYTHWLMYAIGGSHNPTIISQGNRFIAPP-DPNCKEVTKRAYASESEWKSWNWRSEGDLM 427
           DYTHW +YAIGGS  PTI+S GNRFIAPP  P+ +EVTKR YASE EWK WNWRS+ D+ 
Sbjct: 363 DYTHWELYAIGGSQGPTILSHGNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVF 422

Query: 428 MNGAFFIESGSPIRK--YSKKDVITAKPGSFVTRLTRFSGPLKCRNNRPC 441
           MNGA+F +SG+P  K  ++++ +I  K G  V++LT+++G L CR  R C
Sbjct: 423 MNGAYFRQSGNPQYKCAHTRQQMIKPKNGLAVSKLTKYAGALDCRVGRRC 472

BLAST of MS020082 vs. ExPASy Swiss-Prot
Match: Q9SRH4 (Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 3.3e-149
Identity = 260/468 (55.56%), Postives = 329/468 (70.30%), Query Frame = 0

Query: 12  KLLMILSCVMIFPTLKANIAD-FDEVWQKRAQEAKKAAIEAYQPHPEEVTSNFNQQVHKS 71
           KL+ ++    + PT++AN+AD  DE W  +A EA+K  + AY P P E+  +F+++ + +
Sbjct: 8   KLVCVICIASLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDHFHERHYDN 67

Query: 72  ---LDG---------------------ANNTRRHLKHYS-----------GGPCLATNPI 131
              ++G                      N+TRR L                GPC A+NPI
Sbjct: 68  STDVEGTEEEKAVASEEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTGPCTASNPI 127

Query: 132 DRCWRCDKHWAKNRKKLAECALGFGRGTTGGKDGRYYVVRDSSDNDLLNPKPGTLRYGVI 191
           D+CWRC   WA+ RKKL  C  GFG  TTGGK GR YVV    D+D++NP+PGTLR+ VI
Sbjct: 128 DKCWRCQPDWARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTLRHAVI 187

Query: 192 QEKPLWIVFAHDMVIRLSEELIITDNKTIDGRGANVHIANGGQITLQFVKNIIISNLHIH 251
           Q++PLWIVF HDM IRLS+EL+IT +KTID RGANVHIA G  IT+Q+V NIII  LH+H
Sbjct: 188 QKEPLWIVFKHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNIIIHGLHVH 247

Query: 252 DIKNGHGGLIRDSVSHYGLRTPSDGDGISMFGATKIWIDHVSMSNCQDGLIDAVMASTGI 311
            I    GGLIRDS++H+G R  +DGDGIS+FGAT IW+DH+SMS CQDGLIDA+M ST I
Sbjct: 248 HIVKSSGGLIRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLIDAIMGSTAI 307

Query: 312 TISNCHFTRHNDVMLLGASDSYSNDQIMQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDY 371
           TISN HFT HNDVMLLGA ++  +D+ MQVT+A+NHFGKGLVQRMPR RWGFVHVVNNDY
Sbjct: 308 TISNSHFTHHNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHVVNNDY 367

Query: 372 THWLMYAIGGSHNPTIISQGNRFIAPP-DPNCKEVTKRAYASESEWKSWNWRSEGDLMMN 431
           THW +YAIGGS  PTI+S GNRFIAPP   + +EVTKR YASESEWK+WNWRSE D+ MN
Sbjct: 368 THWELYAIGGSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWRSEKDVFMN 427

Query: 432 GAFFIESGSPIRK--YSKKDVITAKPGSFVTRLTRFSGPLKCRNNRPC 441
            A+F +SG+P  K  +S++ +I  K G  V++LT+++G L CR  + C
Sbjct: 428 NAYFRQSGNPHFKCSHSRQQMIKPKNGMAVSKLTKYAGALDCRVGKAC 475

BLAST of MS020082 vs. ExPASy TrEMBL
Match: A0A6J1C763 (Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111009024 PE=3 SV=1)

HSP 1 Score: 867.5 bits (2240), Expect = 2.5e-248
Identity = 408/409 (99.76%), Postives = 409/409 (100.00%), Query Frame = 0

Query: 32  DFDEVWQKRAQEAKKAAIEAYQPHPEEVTSNFNQQVHKSLDGANNTRRHLKHYSGGPCLA 91
           +FDEVWQKRAQEAKKAAIEAYQPHPEEVTSNFNQQVHKSLDGANNTRRHLKHYSGGPCLA
Sbjct: 50  NFDEVWQKRAQEAKKAAIEAYQPHPEEVTSNFNQQVHKSLDGANNTRRHLKHYSGGPCLA 109

Query: 92  TNPIDRCWRCDKHWAKNRKKLAECALGFGRGTTGGKDGRYYVVRDSSDNDLLNPKPGTLR 151
           TNPIDRCWRCDKHWAKNRKKLAECALGFGRGTTGGKDGRYYVVRDSSDNDLLNPKPGTLR
Sbjct: 110 TNPIDRCWRCDKHWAKNRKKLAECALGFGRGTTGGKDGRYYVVRDSSDNDLLNPKPGTLR 169

Query: 152 YGVIQEKPLWIVFAHDMVIRLSEELIITDNKTIDGRGANVHIANGGQITLQFVKNIIISN 211
           YGVIQEKPLWIVFAHDMVIRLSEELIITDNKTIDGRGANVHIANGGQITLQFVKNIIISN
Sbjct: 170 YGVIQEKPLWIVFAHDMVIRLSEELIITDNKTIDGRGANVHIANGGQITLQFVKNIIISN 229

Query: 212 LHIHDIKNGHGGLIRDSVSHYGLRTPSDGDGISMFGATKIWIDHVSMSNCQDGLIDAVMA 271
           LHIHDIKNGHGGLIRDSVSHYGLRTPSDGDGISMFGATKIWIDHVSMSNCQDGLIDAVMA
Sbjct: 230 LHIHDIKNGHGGLIRDSVSHYGLRTPSDGDGISMFGATKIWIDHVSMSNCQDGLIDAVMA 289

Query: 272 STGITISNCHFTRHNDVMLLGASDSYSNDQIMQVTLAFNHFGKGLVQRMPRCRWGFVHVV 331
           STGITISNCHFTRHNDVMLLGASDSYSNDQIMQVTLAFNHFGKGLVQRMPRCRWGFVHVV
Sbjct: 290 STGITISNCHFTRHNDVMLLGASDSYSNDQIMQVTLAFNHFGKGLVQRMPRCRWGFVHVV 349

Query: 332 NNDYTHWLMYAIGGSHNPTIISQGNRFIAPPDPNCKEVTKRAYASESEWKSWNWRSEGDL 391
           NNDYTHWLMYAIGGSHNPTIISQGNRFIAPPDPNCKEVTKRAYASESEWKSWNWRSEGDL
Sbjct: 350 NNDYTHWLMYAIGGSHNPTIISQGNRFIAPPDPNCKEVTKRAYASESEWKSWNWRSEGDL 409

Query: 392 MMNGAFFIESGSPIRKYSKKDVITAKPGSFVTRLTRFSGPLKCRNNRPC 441
           MMNGAFFIESGSPIRKYSKKDVITAKPGSFVTRLTRFSGPLKCRNNRPC
Sbjct: 410 MMNGAFFIESGSPIRKYSKKDVITAKPGSFVTRLTRFSGPLKCRNNRPC 458

BLAST of MS020082 vs. ExPASy TrEMBL
Match: A0A6J1C762 (Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111009024 PE=3 SV=1)

HSP 1 Score: 852.8 bits (2202), Expect = 6.3e-244
Identity = 402/403 (99.75%), Postives = 403/403 (100.00%), Query Frame = 0

Query: 38  QKRAQEAKKAAIEAYQPHPEEVTSNFNQQVHKSLDGANNTRRHLKHYSGGPCLATNPIDR 97
           +KRAQEAKKAAIEAYQPHPEEVTSNFNQQVHKSLDGANNTRRHLKHYSGGPCLATNPIDR
Sbjct: 15  KKRAQEAKKAAIEAYQPHPEEVTSNFNQQVHKSLDGANNTRRHLKHYSGGPCLATNPIDR 74

Query: 98  CWRCDKHWAKNRKKLAECALGFGRGTTGGKDGRYYVVRDSSDNDLLNPKPGTLRYGVIQE 157
           CWRCDKHWAKNRKKLAECALGFGRGTTGGKDGRYYVVRDSSDNDLLNPKPGTLRYGVIQE
Sbjct: 75  CWRCDKHWAKNRKKLAECALGFGRGTTGGKDGRYYVVRDSSDNDLLNPKPGTLRYGVIQE 134

Query: 158 KPLWIVFAHDMVIRLSEELIITDNKTIDGRGANVHIANGGQITLQFVKNIIISNLHIHDI 217
           KPLWIVFAHDMVIRLSEELIITDNKTIDGRGANVHIANGGQITLQFVKNIIISNLHIHDI
Sbjct: 135 KPLWIVFAHDMVIRLSEELIITDNKTIDGRGANVHIANGGQITLQFVKNIIISNLHIHDI 194

Query: 218 KNGHGGLIRDSVSHYGLRTPSDGDGISMFGATKIWIDHVSMSNCQDGLIDAVMASTGITI 277
           KNGHGGLIRDSVSHYGLRTPSDGDGISMFGATKIWIDHVSMSNCQDGLIDAVMASTGITI
Sbjct: 195 KNGHGGLIRDSVSHYGLRTPSDGDGISMFGATKIWIDHVSMSNCQDGLIDAVMASTGITI 254

Query: 278 SNCHFTRHNDVMLLGASDSYSNDQIMQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTH 337
           SNCHFTRHNDVMLLGASDSYSNDQIMQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTH
Sbjct: 255 SNCHFTRHNDVMLLGASDSYSNDQIMQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTH 314

Query: 338 WLMYAIGGSHNPTIISQGNRFIAPPDPNCKEVTKRAYASESEWKSWNWRSEGDLMMNGAF 397
           WLMYAIGGSHNPTIISQGNRFIAPPDPNCKEVTKRAYASESEWKSWNWRSEGDLMMNGAF
Sbjct: 315 WLMYAIGGSHNPTIISQGNRFIAPPDPNCKEVTKRAYASESEWKSWNWRSEGDLMMNGAF 374

Query: 398 FIESGSPIRKYSKKDVITAKPGSFVTRLTRFSGPLKCRNNRPC 441
           FIESGSPIRKYSKKDVITAKPGSFVTRLTRFSGPLKCRNNRPC
Sbjct: 375 FIESGSPIRKYSKKDVITAKPGSFVTRLTRFSGPLKCRNNRPC 417

BLAST of MS020082 vs. ExPASy TrEMBL
Match: A0A5D3BH70 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G003070 PE=3 SV=1)

HSP 1 Score: 820.8 bits (2119), Expect = 2.6e-234
Identity = 380/440 (86.36%), Postives = 420/440 (95.45%), Query Frame = 0

Query: 1   MRETETTIIGLKLLMILSCVMIFPTLKANIADFDEVWQKRAQEAKKAAIEAYQPHPEEVT 60
           MRET+TTI+G+KLLMI   VMIFPTLKANIADFDEVWQKRA EAKKA++EAYQPHPEEVT
Sbjct: 1   MRETKTTIVGIKLLMIFGFVMIFPTLKANIADFDEVWQKRAIEAKKASLEAYQPHPEEVT 60

Query: 61  SNFNQQVHKSLDGANNTRRHLKHYSGGPCLATNPIDRCWRCDKHWAKNRKKLAECALGFG 120
           +NFN+QVH+SLDGANNTRRHL+ Y+ GPCLATNPIDRCWRCD++WA+NRKKLA+CALGFG
Sbjct: 61  NNFNKQVHRSLDGANNTRRHLRKYT-GPCLATNPIDRCWRCDRNWARNRKKLADCALGFG 120

Query: 121 RGTTGGKDGRYYVVRDSSDNDLLNPKPGTLRYGVIQEKPLWIVFAHDMVIRLSEELIITD 180
           RGTTGGKDG+ YVVRDSSDNDL+NPKPGTLR+ VIQE+PLWI+FAHDMVIRLSEELI+TD
Sbjct: 121 RGTTGGKDGKIYVVRDSSDNDLVNPKPGTLRHAVIQERPLWIIFAHDMVIRLSEELIMTD 180

Query: 181 NKTIDGRGANVHIANGGQITLQFVKNIIISNLHIHDIKNGHGGLIRDSVSHYGLRTPSDG 240
           NKT+DGRGANVHIANGGQITLQFV+NIII NLHIHDIK G+GG IRDSVSHYG RT SDG
Sbjct: 181 NKTLDGRGANVHIANGGQITLQFVRNIIIHNLHIHDIKAGNGGFIRDSVSHYGYRTQSDG 240

Query: 241 DGISMFGATKIWIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVMLLGASDSYSND 300
           DGISMFG++++WIDHVSMSNCQDGLIDAVMAST ITISNCHFT HNDV+LLGASDSYSND
Sbjct: 241 DGISMFGSSRVWIDHVSMSNCQDGLIDAVMASTAITISNCHFTHHNDVILLGASDSYSND 300

Query: 301 QIMQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIA 360
           +IMQVTLAFNHFGKGLVQRMPRCRWGF+HVVNNDYTHWLMYAIGGSHNPTIISQGNRFIA
Sbjct: 301 KIMQVTLAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIA 360

Query: 361 PPDPNCKEVTKRAYASESEWKSWNWRSEGDLMMNGAFFIESGSPIRKYSKKDVITAKPGS 420
           PP+PN KEVTKR YASE EW+SWNWRSEGDLMMNGAFFI+SG+PIR+YSKKDVI +KPG+
Sbjct: 361 PPNPNSKEVTKRVYASEREWRSWNWRSEGDLMMNGAFFIQSGNPIRRYSKKDVIHSKPGT 420

Query: 421 FVTRLTRFSGPLKCRNNRPC 441
           FVTRLTRF+GPLKC+ N+PC
Sbjct: 421 FVTRLTRFAGPLKCKMNQPC 439

BLAST of MS020082 vs. ExPASy TrEMBL
Match: A0A0A0LR32 (Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_1G045510 PE=3 SV=1)

HSP 1 Score: 813.9 bits (2101), Expect = 3.2e-232
Identity = 375/440 (85.23%), Postives = 417/440 (94.77%), Query Frame = 0

Query: 1   MRETETTIIGLKLLMILSCVMIFPTLKANIADFDEVWQKRAQEAKKAAIEAYQPHPEEVT 60
           MRET+TTI+G+KLLMI   VMIFPTLKANIADFDEVWQKRA EA+KA+ EAY+PHPEE T
Sbjct: 1   MRETKTTIVGIKLLMIFGFVMIFPTLKANIADFDEVWQKRAIEAQKASFEAYEPHPEEET 60

Query: 61  SNFNQQVHKSLDGANNTRRHLKHYSGGPCLATNPIDRCWRCDKHWAKNRKKLAECALGFG 120
           +NFN+QVH+SLDG NNTRRHL+ Y+ GPCLATNPIDRCWRCD++WA+NRKKLA+CALGFG
Sbjct: 61  NNFNKQVHRSLDGGNNTRRHLRKYT-GPCLATNPIDRCWRCDRNWARNRKKLADCALGFG 120

Query: 121 RGTTGGKDGRYYVVRDSSDNDLLNPKPGTLRYGVIQEKPLWIVFAHDMVIRLSEELIITD 180
           R TTGGKDG+ YVVRDSSDNDL+NPKPGTLR+ VIQE+PLWI+FAHDMVIRLSEELI+TD
Sbjct: 121 RRTTGGKDGKIYVVRDSSDNDLVNPKPGTLRHAVIQERPLWIIFAHDMVIRLSEELIVTD 180

Query: 181 NKTIDGRGANVHIANGGQITLQFVKNIIISNLHIHDIKNGHGGLIRDSVSHYGLRTPSDG 240
           +KT+DGRGANVHIANGGQITLQFVKNIII NLHIHDIK G+GG+IRDSVSHYG RT SDG
Sbjct: 181 DKTLDGRGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGMIRDSVSHYGFRTRSDG 240

Query: 241 DGISMFGATKIWIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVMLLGASDSYSND 300
           DGISMFGA+++WIDHVSMSNCQDGLIDAVMAST ITISNCHFT HNDV+LLGAS+ YSND
Sbjct: 241 DGISMFGASRVWIDHVSMSNCQDGLIDAVMASTAITISNCHFTHHNDVILLGASNGYSND 300

Query: 301 QIMQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIA 360
           QIMQVTLAFNHFGKGLVQRMPRCRWGF+HVVNNDYTHWLMYAIGGSHNPTIISQGNRFIA
Sbjct: 301 QIMQVTLAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIA 360

Query: 361 PPDPNCKEVTKRAYASESEWKSWNWRSEGDLMMNGAFFIESGSPIRKYSKKDVITAKPGS 420
           PP+PNCKEVTKR YA ESEW+SWNWRSEGDLMMNGAFFI+SG+PI++YSKKDVI +KPG+
Sbjct: 361 PPNPNCKEVTKRVYAPESEWRSWNWRSEGDLMMNGAFFIQSGNPIKRYSKKDVIHSKPGT 420

Query: 421 FVTRLTRFSGPLKCRNNRPC 441
           FVTRLTRF+GPLKC+ N+PC
Sbjct: 421 FVTRLTRFAGPLKCKKNQPC 439

BLAST of MS020082 vs. ExPASy TrEMBL
Match: A0A6J1K214 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111489953 PE=3 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 2.0e-226
Identity = 370/441 (83.90%), Postives = 412/441 (93.42%), Query Frame = 0

Query: 1   MRETETTIIGLK-LLMILSCVMIFPTLKANIADFDEVWQKRAQEAKKAAIEAYQPHPEEV 60
           MRE E+ I+G K LL+I +  +IFPTL+A+IADFDEVWQ+RAQEAKKAA+EAYQPHPEEV
Sbjct: 1   MRELESNIVGAKLLLLIFAFAIIFPTLEADIADFDEVWQQRAQEAKKAALEAYQPHPEEV 60

Query: 61  TSNFNQQVHKSLDGANNTRRHLKHYSGGPCLATNPIDRCWRCDKHWAKNRKKLAECALGF 120
           T NFN+ V+  LD ANNTRRHL+ Y+ GPCLATNPIDRCWRCDK+WAKNRKKLA+CALGF
Sbjct: 61  TENFNEHVNGDLDDANNTRRHLRKYN-GPCLATNPIDRCWRCDKNWAKNRKKLADCALGF 120

Query: 121 GRGTTGGKDGRYYVVRDSSDNDLLNPKPGTLRYGVIQEKPLWIVFAHDMVIRLSEELIIT 180
           GRGTTGGK+GR+YVV+DSSDND+L+PKPGTLRY VIQ++PLWI FAHDMVIRL+EELI+T
Sbjct: 121 GRGTTGGKNGRFYVVQDSSDNDMLHPKPGTLRYAVIQDRPLWITFAHDMVIRLAEELIMT 180

Query: 181 DNKTIDGRGANVHIANGGQITLQFVKNIIISNLHIHDIKNGHGGLIRDSVSHYGLRTPSD 240
           DNKT+DGRGANVHIANGGQITLQFVKNIII NLHIHDIK G+GGLIRDSV+HYG+RT SD
Sbjct: 181 DNKTLDGRGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGLIRDSVNHYGIRTRSD 240

Query: 241 GDGISMFGATKIWIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVMLLGASDSYSN 300
           GDGIS+FGA+ +WIDHVSMSNCQDGLIDAVMAST ITISNCHF  HNDV+LLGASDS+S+
Sbjct: 241 GDGISIFGASNVWIDHVSMSNCQDGLIDAVMASTAITISNCHFAHHNDVILLGASDSHSD 300

Query: 301 DQIMQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSHNPTIISQGNRFI 360
           DQIMQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGS NPTIISQGNRFI
Sbjct: 301 DQIMQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSRNPTIISQGNRFI 360

Query: 361 APPDPNCKEVTKRAYASESEWKSWNWRSEGDLMMNGAFFIESGSPIRKYSKKDVITAKPG 420
           APPDPNCKEVTKR YA ES+WK+WNWRSEGDL+MNGAFF+ESG+PIRKYSKKDVI AKPG
Sbjct: 361 APPDPNCKEVTKRVYAPESDWKTWNWRSEGDLLMNGAFFVESGNPIRKYSKKDVIHAKPG 420

Query: 421 SFVTRLTRFSGPLKCRNNRPC 441
           +FVTRLTRF+GPLKC  N+PC
Sbjct: 421 TFVTRLTRFAGPLKCTRNQPC 440

BLAST of MS020082 vs. TAIR 10
Match: AT1G14420.1 (Pectate lyase family protein )

HSP 1 Score: 537.3 bits (1383), Expect = 1.1e-152
Identity = 253/438 (57.76%), Postives = 316/438 (72.15%), Query Frame = 0

Query: 21  MIFPTLKANIADFDEVWQKRAQEAKKAAIEAYQPHPEEVTSNFNQQVHKSLDGA---NNT 80
           ++ P ++ N+A FD  W +R  +A K  I +Y PHP  VT++FN  V+ ++D +   N+T
Sbjct: 22  IVAPQVRGNVAVFDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNIAVDASESRNDT 81

Query: 81  RRHL--------KHYSGGPCLATNPIDRCWRCDKHWAKNRKKLAECALGFGRGTTGGKDG 140
           RR L         H S G CLA NPID CWRCD++WA NRKKLA+C LGFGR TTGGKDG
Sbjct: 82  RRELTQVRSGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFGRRTTGGKDG 141

Query: 141 RYYVVRDSSDNDLLNPKPGTLRYGVIQEKPLWIVFAHDMVIRLSEELIITDNKTIDGRGA 200
             YVV+D+SDNDL+NPKPGTLR+ V ++ PLWI+FA  M+I+L +EL+IT +KTIDGRGA
Sbjct: 142 PIYVVKDASDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITSDKTIDGRGA 201

Query: 201 NVHIANGGQITLQFVKNIIISNLHIHDIKNGHGGLIRDSVSHYGLRTPSDGDGISMFGAT 260
            V+I  G  +TLQFV N+II N+++  I  G+GGLIRDS +H GLRT SDGDGIS+FGAT
Sbjct: 202 RVYIMEGAGLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDGDGISLFGAT 261

Query: 261 KIWIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVMLLGASDSYSNDQIMQVTLAF 320
            IWIDHVSM+ C DG+IDA+  ST +TISN HFT H +VML GA D +  D+ MQ+T+AF
Sbjct: 262 NIWIDHVSMTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVIDKKMQITVAF 321

Query: 321 NHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPDPNCKEV 380
           NHFGK L QRMPRCR+G +HVVNNDYTHW MYAIGG+ NPTIISQGNRFIAPP+   K++
Sbjct: 322 NHFGKRLEQRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPNEEAKQI 381

Query: 381 TKRAYASESEWKSWNWRSEGDLMMNGAFFIESG-------SPIRKYSKKDVITAKPGSFV 440
           TKR Y    EWKSWNW+SEGD  +NGA+F++SG        P      K  I  KPG+ V
Sbjct: 382 TKREYTPYGEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFTIRPKPGTMV 441

BLAST of MS020082 vs. TAIR 10
Match: AT5G15110.1 (Pectate lyase family protein )

HSP 1 Score: 533.9 bits (1374), Expect = 1.2e-151
Identity = 255/470 (54.26%), Postives = 334/470 (71.06%), Query Frame = 0

Query: 8   IIGLKLLMILSCV-MIFPTLKANIADFDEVWQKRAQEAKKAAIEAYQPHPEEVTSNFNQQ 67
           ++ L  LM   C+ ++ PT++ NI++ DE W +RA EA++  ++AY   P E+  +F+++
Sbjct: 3   MVRLSKLMFTFCIAVLIPTIRGNISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHER 62

Query: 68  VHKSL--------DG------------------ANNTRRHLKHYS-------GGPCLATN 127
            + +         DG                   N+TRR L+           GPC A+N
Sbjct: 63  HYDNSTDVTTPEEDGDAKPEEEEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASN 122

Query: 128 PIDRCWRCDKHWAKNRKKLAECALGFGRGTTGGKDGRYYVVRDSSDNDLLNPKPGTLRYG 187
           PID+CWRC   WAK RKKL  C  GFG  TTGGK GR YVV  + D D++NPKPGTLR+ 
Sbjct: 123 PIDKCWRCRSDWAKRRKKLTRCVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHA 182

Query: 188 VIQEKPLWIVFAHDMVIRLSEELIITDNKTIDGRGANVHIANGGQITLQFVKNIIISNLH 247
           VIQ++PLWI+F +DM IRL++EL+I  +KTID RGANVH+A+G  IT+QFVKN++I  LH
Sbjct: 183 VIQKEPLWIIFKNDMSIRLNQELLINSHKTIDARGANVHVAHGAGITMQFVKNVVIHGLH 242

Query: 248 IHDIKNGHGGLIRDSVSHYGLRTPSDGDGISMFGATKIWIDHVSMSNCQDGLIDAVMAST 307
           IH I    GG+IRDSV H+G+RT +DGDG+S++G++ IW+DH+SMS CQDGLIDA++ ST
Sbjct: 243 IHHISESSGGMIRDSVDHFGMRTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGST 302

Query: 308 GITISNCHFTRHNDVMLLGASDSYSNDQIMQVTLAFNHFGKGLVQRMPRCRWGFVHVVNN 367
           GITISN HFT HNDVMLLGA ++   D+ MQVT+A+NHFGKGLVQRMPR RWGFVHVVNN
Sbjct: 303 GITISNSHFTHHNDVMLLGAQNTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNN 362

Query: 368 DYTHWLMYAIGGSHNPTIISQGNRFIAPP-DPNCKEVTKRAYASESEWKSWNWRSEGDLM 427
           DYTHW +YAIGGS  PTI+S GNRFIAPP  P+ +EVTKR YASE EWK WNWRS+ D+ 
Sbjct: 363 DYTHWELYAIGGSQGPTILSHGNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVF 422

Query: 428 MNGAFFIESGSPIRK--YSKKDVITAKPGSFVTRLTRFSGPLKCRNNRPC 441
           MNGA+F +SG+P  K  ++++ +I  K G  V++LT+++G L CR  R C
Sbjct: 423 MNGAYFRQSGNPQYKCAHTRQQMIKPKNGLAVSKLTKYAGALDCRVGRRC 472

BLAST of MS020082 vs. TAIR 10
Match: AT3G01270.1 (Pectate lyase family protein )

HSP 1 Score: 529.6 bits (1363), Expect = 2.3e-150
Identity = 260/468 (55.56%), Postives = 329/468 (70.30%), Query Frame = 0

Query: 12  KLLMILSCVMIFPTLKANIAD-FDEVWQKRAQEAKKAAIEAYQPHPEEVTSNFNQQVHKS 71
           KL+ ++    + PT++AN+AD  DE W  +A EA+K  + AY P P E+  +F+++ + +
Sbjct: 8   KLVCVICIASLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDHFHERHYDN 67

Query: 72  ---LDG---------------------ANNTRRHLKHYS-----------GGPCLATNPI 131
              ++G                      N+TRR L                GPC A+NPI
Sbjct: 68  STDVEGTEEEKAVASEEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTGPCTASNPI 127

Query: 132 DRCWRCDKHWAKNRKKLAECALGFGRGTTGGKDGRYYVVRDSSDNDLLNPKPGTLRYGVI 191
           D+CWRC   WA+ RKKL  C  GFG  TTGGK GR YVV    D+D++NP+PGTLR+ VI
Sbjct: 128 DKCWRCQPDWARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTLRHAVI 187

Query: 192 QEKPLWIVFAHDMVIRLSEELIITDNKTIDGRGANVHIANGGQITLQFVKNIIISNLHIH 251
           Q++PLWIVF HDM IRLS+EL+IT +KTID RGANVHIA G  IT+Q+V NIII  LH+H
Sbjct: 188 QKEPLWIVFKHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNIIIHGLHVH 247

Query: 252 DIKNGHGGLIRDSVSHYGLRTPSDGDGISMFGATKIWIDHVSMSNCQDGLIDAVMASTGI 311
            I    GGLIRDS++H+G R  +DGDGIS+FGAT IW+DH+SMS CQDGLIDA+M ST I
Sbjct: 248 HIVKSSGGLIRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLIDAIMGSTAI 307

Query: 312 TISNCHFTRHNDVMLLGASDSYSNDQIMQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDY 371
           TISN HFT HNDVMLLGA ++  +D+ MQVT+A+NHFGKGLVQRMPR RWGFVHVVNNDY
Sbjct: 308 TISNSHFTHHNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHVVNNDY 367

Query: 372 THWLMYAIGGSHNPTIISQGNRFIAPP-DPNCKEVTKRAYASESEWKSWNWRSEGDLMMN 431
           THW +YAIGGS  PTI+S GNRFIAPP   + +EVTKR YASESEWK+WNWRSE D+ MN
Sbjct: 368 THWELYAIGGSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWRSEKDVFMN 427

Query: 432 GAFFIESGSPIRK--YSKKDVITAKPGSFVTRLTRFSGPLKCRNNRPC 441
            A+F +SG+P  K  +S++ +I  K G  V++LT+++G L CR  + C
Sbjct: 428 NAYFRQSGNPHFKCSHSRQQMIKPKNGMAVSKLTKYAGALDCRVGKAC 475

BLAST of MS020082 vs. TAIR 10
Match: AT2G02720.1 (Pectate lyase family protein )

HSP 1 Score: 515.0 bits (1325), Expect = 6.0e-146
Identity = 246/446 (55.16%), Postives = 320/446 (71.75%), Query Frame = 0

Query: 13  LLMILSCVMIFPTLKANIADFDEVWQKRAQEAKKAAIEAYQPHPEEVTSNFNQQVHKSLD 72
           + + LS  ++ P+++A+IA++DE W +R   A +  +E+Y P+PE VT +FN     +++
Sbjct: 10  VFVALSLTVVVPSVQAHIAEYDEYWTQRQTNALRETLESYDPNPENVTDHFNYHAALAME 69

Query: 73  G---ANNTRRHLKHYS--------GGPCLATNPIDRCWRCDKHWAKNRKKLAECALGFGR 132
                N TRR L+           GG   + N ID+CWR DK+W KNRKKLA+C LGFGR
Sbjct: 70  TTGIVNETRRDLRQVGRGKKTTRRGGRFESLNAIDKCWRGDKNWDKNRKKLADCVLGFGR 129

Query: 133 GTTGGKDGRYYVVRDSSDNDLLNPKPGTLRYGVIQEKPLWIVFAHDMVIRLSEELIITDN 192
            TTGGK+G  YVV D SDNDLL PKPGT+R+ V +++PLWI+FA  M+I+L +ELIIT++
Sbjct: 130 KTTGGKNGPIYVVTDPSDNDLLKPKPGTIRHAVTRDRPLWIIFARSMIIKLQQELIITND 189

Query: 193 KTIDGRGANVHIANGGQITLQFVKNIIISNLHIHDIKNGHGGLIRDSVSHYGLRTPSDGD 252
           KTIDGRGA ++I  G  +TLQFV+N+II N+HI  IK G GGLI DS  H+GLRT SDGD
Sbjct: 190 KTIDGRGAKIYITGGAGLTLQFVRNVIIHNIHIKQIKRGAGGLIIDSEQHFGLRTVSDGD 249

Query: 253 GISMFGATKIWIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVMLLGASDSYSNDQ 312
           GI++FGAT +WIDHVSM++C DG+IDA+M ST ITISN HFT H++VML G ++    D+
Sbjct: 250 GINIFGATNVWIDHVSMTDCSDGMIDAIMGSTAITISNSHFTDHDEVMLFGGTNKDVIDK 309

Query: 313 IMQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAP 372
            MQ+T+AFNHFGK L QRMPR R+G VHVVNNDYTHW MYAIGG+ NPTIISQGNRFIAP
Sbjct: 310 KMQITVAFNHFGKRLKQRMPRVRFGLVHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAP 369

Query: 373 PDPNCKEVTKRAYASESEWKSWNWRSEGDLMMNGAFFIESG-------SPIRKYSKKDVI 432
           P  + K+VTKR Y    EWKSWNW+SE D  +NGA+F++SG       +P     +K  I
Sbjct: 370 PIEDSKQVTKREYTPYPEWKSWNWQSEKDYFLNGAYFVQSGKANAWSATPKNPIPRKFAI 429

Query: 433 TAKPGSFVTRLTRFSGPLKCRNNRPC 441
             +PG+ V RLT+ +G L C+  + C
Sbjct: 430 RPQPGTKVRRLTKDAGTLGCKPGKSC 455

BLAST of MS020082 vs. TAIR 10
Match: AT5G48900.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 490.7 bits (1262), Expect = 1.2e-138
Identity = 237/408 (58.09%), Postives = 290/408 (71.08%), Query Frame = 0

Query: 34  DEVWQKRAQEAKKAAIEAYQPHPEEVTSNFNQQVHKSLDGANNTRRHLKHYSGGPCLATN 93
           +E W + A E           +PEEV +  +  +  S       RR L ++S   C   N
Sbjct: 29  NETWHEHAVE-----------NPEEVAAMVDMSIRNS-----TARRRLGYFS---CSTGN 88

Query: 94  PIDRCWRCDKHWAKNRKKLAECALGFGRGTTGGKDGRYYVVRDSSDNDLLNPKPGTLRYG 153
           PID CWRCD+ W   RK LA CA+GFGR   GG+DGRYYVV D +D++ +NPKPGTLR+ 
Sbjct: 89  PIDDCWRCDRRWQSRRKHLANCAIGFGRNAIGGRDGRYYVVSDPNDDNPVNPKPGTLRHA 148

Query: 154 VIQEKPLWIVFAHDMVIRLSEELIITDNKTIDGRGANVHIANGGQITLQFVKNIIISNLH 213
           VIQE+PLWIVF  DMVI L EELI+   KTIDGRG NVHIANG  IT+QFV NIII  +H
Sbjct: 149 VIQEEPLWIVFKRDMVITLKEELIMNSFKTIDGRGVNVHIANGACITIQFVTNIIIHGIH 208

Query: 214 IHDIKNGHGGLIRDSVSHYGLRTPSDGDGISMFGATKIWIDHVSMSNCQDGLIDAVMAST 273
           IHD +     ++R S SHYG RT +DGDGIS+FG++ IWIDH S+SNC DGLIDAVMAST
Sbjct: 209 IHDCRPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSNCADGLIDAVMAST 268

Query: 274 GITISNCHFTRHNDVMLLGASDSYSNDQIMQVTLAFNHFGKGLVQRMPRCRWGFVHVVNN 333
            ITISN +FT HN+VMLLG SD+Y+ D++MQVT+A+NHFG+GL+QRMPRCR G+ HVVNN
Sbjct: 269 AITISNNYFTHHNEVMLLGHSDTYTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNN 328

Query: 334 DYTHWLMYAIGGSHNPTIISQGNRFIAPPDPNCKEVTKRAYASESEWKSWNWRSEGDLMM 393
           DYTHW MYAIGGS +PTI SQGNR++AP +   KEVTKR YA + +W+ WNWRSEGDL +
Sbjct: 329 DYTHWEMYAIGGSASPTINSQGNRYLAPRNRFAKEVTKRDYAGQWQWRHWNWRSEGDLFL 388

Query: 394 NGAFFIESGSPI-RKYSKKDVITAKPGSFVTRLTRFSGPLKCRNNRPC 441
           NGAFF  SGS +   Y++   + AK  S V  +T  +G L CR  R C
Sbjct: 389 NGAFFTRSGSGLGASYARASSLAAKSSSLVGVITYNAGALNCRGGRRC 417