MS020048 (gene) Bitter gourd (TR) v1

Overview
NameMS020048
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationscaffold22: 1393518 .. 1394972 (-)
RNA-Seq ExpressionMS020048
SyntenyMS020048
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AATCACAGGCTCAAACTTTCGCTCTTTCACCACAGATCTCCATCAGAATCCAAGCCCGGACGCCCCAAAGAATATCCCCAGGAGTGCCTGTGCCCGATTTCCAACTCATTAATGGCGGACCCAGTTGTGGTCTCCTCTGGCCACACCTTCGACCGCCTCTGCGTCCAAGTATGTCAAGATTTGGGGTTCTTGCCTAAGCTTGAAGATGGGTCAACCCCCGATTTCTCCTCGGTTATTACCAACAGAAACGTGAGATTCACCATTCTCAAGTGGTGCGATAACAACGGAATTGAACACCCGCGAGAGCCCGATTACAGGTCCATTGAGCTCATTGTTCGCCAGTTAATGGAGAAGGAGAGACGAGAGAATCGATTCGAGGTTTCCGATAGCGAGCTGTTGAGGGGCGTGGCCGATAAACCGCAGGGGGTTTTGAATCACGCCACGACGGAGGTGGGTCTCCGCCGATTACACCTCAACTCGCCGCCGCAGGGGGAGGAGATCGTCCGGGAGAGTACTCTTCTGCCCTTCAAAACCCAGCCCTCTTGTTATGCCTCTTCCTCTTCCAATGAAAGCTTCGAAAAAGTGCCCAATCACCGCGGAGAATCAAATTCCCCAAACTCCGGAGAAGAATCGCGACTCATCTCGAAATTCACGAGCGCCGAACCATTCGAGCAAGAAGACGGAGTAGTTTCCTTAAGAAGAATGACGAAATCCGACGAATCGATTAGGGTTTCGCTCTGTACAAAAGAGTTACTCGCCGCCCTCCTTCCTCTGGTTTCCTCCCGATCGTCCAATGTACAGATCAACGCTGTAGCTTCCGTGGTCAATCTCTCCCTCGATAAATCGAACAAACTGAAGATCGTACGGGCCGGATTCGTTCCGCCATTAATCGACGTTTTGGACAGTGGATTCGACGAACCACAGGAGCACGCCGCCGGTGCCCTGTTCAGCCTATCATTAGACGACGAGAACAAAATGGCGATAGGAATTCTCGGCGCGTTACCGGTACTGATGAACACGGTCCGATCCGACAGCGAGCGGACGAGGCACGACTCAGCCCTCTGTTTGTACCATTTAACTTTAAACCCTAGCAACCGAGTCAAACTCGTGAAACTCGGCGCCGTCCCAATACTACTATCGTTGGCGAAATTGGAAGACTCGGCTAGCCGTATACTGCTGATCCTGTGCAATATCGCAGTCTCCGTCGACGGAAGATCGGCAATGCTGGACGCGAACGCGGTGGGTTGCTTGGTGGGGATTCTGAAGGAGAAGACGATGGATTCCGAGTCGACTCGGGAGAATTGCGTGGTGGCGCTGCACGCGCTGAGTCAGGGCGGGTTCCGATTCAGAGGACTGGCGAAGGAGGCCGGCGCGGTGGAGGTTCTGAGAGAGATTGAAGAGAGGGGAAGCGAGAGGGCGAGGGAGAAGGCCAAGAGGATTTTGCAGATGATGAGG

mRNA sequence

AATCACAGGCTCAAACTTTCGCTCTTTCACCACAGATCTCCATCAGAATCCAAGCCCGGACGCCCCAAAGAATATCCCCAGGAGTGCCTGTGCCCGATTTCCAACTCATTAATGGCGGACCCAGTTGTGGTCTCCTCTGGCCACACCTTCGACCGCCTCTGCGTCCAAGTATGTCAAGATTTGGGGTTCTTGCCTAAGCTTGAAGATGGGTCAACCCCCGATTTCTCCTCGGTTATTACCAACAGAAACGTGAGATTCACCATTCTCAAGTGGTGCGATAACAACGGAATTGAACACCCGCGAGAGCCCGATTACAGGTCCATTGAGCTCATTGTTCGCCAGTTAATGGAGAAGGAGAGACGAGAGAATCGATTCGAGGTTTCCGATAGCGAGCTGTTGAGGGGCGTGGCCGATAAACCGCAGGGGGTTTTGAATCACGCCACGACGGAGGTGGGTCTCCGCCGATTACACCTCAACTCGCCGCCGCAGGGGGAGGAGATCGTCCGGGAGAGTACTCTTCTGCCCTTCAAAACCCAGCCCTCTTGTTATGCCTCTTCCTCTTCCAATGAAAGCTTCGAAAAAGTGCCCAATCACCGCGGAGAATCAAATTCCCCAAACTCCGGAGAAGAATCGCGACTCATCTCGAAATTCACGAGCGCCGAACCATTCGAGCAAGAAGACGGAGTAGTTTCCTTAAGAAGAATGACGAAATCCGACGAATCGATTAGGGTTTCGCTCTGTACAAAAGAGTTACTCGCCGCCCTCCTTCCTCTGGTTTCCTCCCGATCGTCCAATGTACAGATCAACGCTGTAGCTTCCGTGGTCAATCTCTCCCTCGATAAATCGAACAAACTGAAGATCGTACGGGCCGGATTCGTTCCGCCATTAATCGACGTTTTGGACAGTGGATTCGACGAACCACAGGAGCACGCCGCCGGTGCCCTGTTCAGCCTATCATTAGACGACGAGAACAAAATGGCGATAGGAATTCTCGGCGCGTTACCGGTACTGATGAACACGGTCCGATCCGACAGCGAGCGGACGAGGCACGACTCAGCCCTCTGTTTGTACCATTTAACTTTAAACCCTAGCAACCGAGTCAAACTCGTGAAACTCGGCGCCGTCCCAATACTACTATCGTTGGCGAAATTGGAAGACTCGGCTAGCCGTATACTGCTGATCCTGTGCAATATCGCAGTCTCCGTCGACGGAAGATCGGCAATGCTGGACGCGAACGCGGTGGGTTGCTTGGTGGGGATTCTGAAGGAGAAGACGATGGATTCCGAGTCGACTCGGGAGAATTGCGTGGTGGCGCTGCACGCGCTGAGTCAGGGCGGGTTCCGATTCAGAGGACTGGCGAAGGAGGCCGGCGCGGTGGAGGTTCTGAGAGAGATTGAAGAGAGGGGAAGCGAGAGGGCGAGGGAGAAGGCCAAGAGGATTTTGCAGATGATGAGG

Coding sequence (CDS)

AATCACAGGCTCAAACTTTCGCTCTTTCACCACAGATCTCCATCAGAATCCAAGCCCGGACGCCCCAAAGAATATCCCCAGGAGTGCCTGTGCCCGATTTCCAACTCATTAATGGCGGACCCAGTTGTGGTCTCCTCTGGCCACACCTTCGACCGCCTCTGCGTCCAAGTATGTCAAGATTTGGGGTTCTTGCCTAAGCTTGAAGATGGGTCAACCCCCGATTTCTCCTCGGTTATTACCAACAGAAACGTGAGATTCACCATTCTCAAGTGGTGCGATAACAACGGAATTGAACACCCGCGAGAGCCCGATTACAGGTCCATTGAGCTCATTGTTCGCCAGTTAATGGAGAAGGAGAGACGAGAGAATCGATTCGAGGTTTCCGATAGCGAGCTGTTGAGGGGCGTGGCCGATAAACCGCAGGGGGTTTTGAATCACGCCACGACGGAGGTGGGTCTCCGCCGATTACACCTCAACTCGCCGCCGCAGGGGGAGGAGATCGTCCGGGAGAGTACTCTTCTGCCCTTCAAAACCCAGCCCTCTTGTTATGCCTCTTCCTCTTCCAATGAAAGCTTCGAAAAAGTGCCCAATCACCGCGGAGAATCAAATTCCCCAAACTCCGGAGAAGAATCGCGACTCATCTCGAAATTCACGAGCGCCGAACCATTCGAGCAAGAAGACGGAGTAGTTTCCTTAAGAAGAATGACGAAATCCGACGAATCGATTAGGGTTTCGCTCTGTACAAAAGAGTTACTCGCCGCCCTCCTTCCTCTGGTTTCCTCCCGATCGTCCAATGTACAGATCAACGCTGTAGCTTCCGTGGTCAATCTCTCCCTCGATAAATCGAACAAACTGAAGATCGTACGGGCCGGATTCGTTCCGCCATTAATCGACGTTTTGGACAGTGGATTCGACGAACCACAGGAGCACGCCGCCGGTGCCCTGTTCAGCCTATCATTAGACGACGAGAACAAAATGGCGATAGGAATTCTCGGCGCGTTACCGGTACTGATGAACACGGTCCGATCCGACAGCGAGCGGACGAGGCACGACTCAGCCCTCTGTTTGTACCATTTAACTTTAAACCCTAGCAACCGAGTCAAACTCGTGAAACTCGGCGCCGTCCCAATACTACTATCGTTGGCGAAATTGGAAGACTCGGCTAGCCGTATACTGCTGATCCTGTGCAATATCGCAGTCTCCGTCGACGGAAGATCGGCAATGCTGGACGCGAACGCGGTGGGTTGCTTGGTGGGGATTCTGAAGGAGAAGACGATGGATTCCGAGTCGACTCGGGAGAATTGCGTGGTGGCGCTGCACGCGCTGAGTCAGGGCGGGTTCCGATTCAGAGGACTGGCGAAGGAGGCCGGCGCGGTGGAGGTTCTGAGAGAGATTGAAGAGAGGGGAAGCGAGAGGGCGAGGGAGAAGGCCAAGAGGATTTTGCAGATGATGAGG

Protein sequence

NHRLKLSLFHHRSPSESKPGRPKEYPQECLCPISNSLMADPVVVSSGHTFDRLCVQVCQDLGFLPKLEDGSTPDFSSVITNRNVRFTILKWCDNNGIEHPREPDYRSIELIVRQLMEKERRENRFEVSDSELLRGVADKPQGVLNHATTEVGLRRLHLNSPPQGEEIVRESTLLPFKTQPSCYASSSSNESFEKVPNHRGESNSPNSGEESRLISKFTSAEPFEQEDGVVSLRRMTKSDESIRVSLCTKELLAALLPLVSSRSSNVQINAVASVVNLSLDKSNKLKIVRAGFVPPLIDVLDSGFDEPQEHAAGALFSLSLDDENKMAIGILGALPVLMNTVRSDSERTRHDSALCLYHLTLNPSNRVKLVKLGAVPILLSLAKLEDSASRILLILCNIAVSVDGRSAMLDANAVGCLVGILKEKTMDSESTRENCVVALHALSQGGFRFRGLAKEAGAVEVLREIEERGSERAREKAKRILQMMR
Homology
BLAST of MS020048 vs. NCBI nr
Match: XP_038895164.1 (U-box domain-containing protein 38-like [Benincasa hispida])

HSP 1 Score: 759.2 bits (1959), Expect = 2.1e-215
Identity = 410/495 (82.83%), Postives = 441/495 (89.09%), Query Frame = 0

Query: 1   NHRLKLSLFHHRSPSESKPGRPKEYPQECLCPISNSLMADPVVVSSGHTFDRLCVQVCQD 60
           NHRLKLS+FHHRS S+SKP RPKEYP+E LC IS SLMADPVVVSSG TF+RL VQVCQD
Sbjct: 6   NHRLKLSIFHHRSSSDSKPERPKEYPEEYLCAISKSLMADPVVVSSGQTFERLSVQVCQD 65

Query: 61  LGFLPKL-EDGSTPDFSSVITNRNVRFTILKWCDNNGIEHPREPDYRSIELIVRQLMEKE 120
           LGF P+L EDGS PDFSSVITNRN+R TILKWCDNNGIEHP+ PDY SIELIVRQLMEKE
Sbjct: 66  LGFSPELEEDGSRPDFSSVITNRNMRSTILKWCDNNGIEHPQPPDYSSIELIVRQLMEKE 125

Query: 121 RRENRFEVSDSELLRGVADKPQGVLNHATTEVG--LRRLHLNSPPQGEEIVRESTLLPFK 180
           RR+NRFE SDSEL+RGVADKPQGV  HATTEVG  L R  LNSPPQ EEIVRESTLLPFK
Sbjct: 126 RRKNRFEASDSELIRGVADKPQGVAVHATTEVGRRLNRFQLNSPPQTEEIVRESTLLPFK 185

Query: 181 TQPSCYA---SSSSNESF--EKVPNHRG--ESNSPNSGEESRLISKFTSAEPFEQEDGVV 240
           TQPS YA   SSSS+ SF  E+ PNHRG  +S SPNS EESRLI KF S++ +EQ +GV+
Sbjct: 186 TQPSSYAAHSSSSSHGSFDRERDPNHRGYSDSYSPNSEEESRLILKFKSSDEYEQREGVI 245

Query: 241 SLRRMTKSDESIRVSLCTKELLAALLPLVSSRSSNVQINAVASVVNLSLDKSNKLKIVRA 300
           SLR++TKS+ESIR SLCTKE LAALLPLV SRS+N+QINAVASVVNLSL+K NKLKIVRA
Sbjct: 246 SLRKLTKSNESIRGSLCTKEFLAALLPLVLSRSANIQINAVASVVNLSLEKPNKLKIVRA 305

Query: 301 GFVPPLIDVLDSGFDEPQEHAAGALFSLSLDDENKMAIGILGALPVLMNTVRSDSERTRH 360
           GFVPPLIDVLD G  E QEHAAGALFSLS DDENKMAIGILGALPVLMNT+RSDSERTR+
Sbjct: 306 GFVPPLIDVLDGGNTESQEHAAGALFSLSSDDENKMAIGILGALPVLMNTLRSDSERTRN 365

Query: 361 DSALCLYHLTLNPSNRVKLVKLGAVPILLSLAKLEDSASRILLILCNIAVSVDGRSAMLD 420
           DSALCLYHLTLNPSNRVKLVKLGAVPILLSL ++E   SRILLILCNIAVSVDGRSAMLD
Sbjct: 366 DSALCLYHLTLNPSNRVKLVKLGAVPILLSLTRIEGFTSRILLILCNIAVSVDGRSAMLD 425

Query: 421 ANAVGCLVGILKEKTMDSESTRENCVVALHALSQGGFRFRGLAKEAGAVEVLREIEERGS 480
           ANAVGCLV ILK+K MDSESTRENCVVAL+ALSQGGFRFRGLAKEAGAVEVLRE+E RGS
Sbjct: 426 ANAVGCLVEILKDKDMDSESTRENCVVALYALSQGGFRFRGLAKEAGAVEVLREVEGRGS 485

Query: 481 ERAREKAKRILQMMR 486
           ERAREKA+RILQMMR
Sbjct: 486 ERAREKARRILQMMR 500

BLAST of MS020048 vs. NCBI nr
Match: XP_022923679.1 (U-box domain-containing protein 38-like [Cucurbita moschata] >KAG7019833.1 U-box domain-containing protein 38, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 748.4 bits (1931), Expect = 3.8e-212
Identity = 395/490 (80.61%), Postives = 438/490 (89.39%), Query Frame = 0

Query: 1   NHRLKLSLFHHRSPSESKPGRPKEYPQECLCPISNSLMADPVVVSSGHTFDRLCVQVCQD 60
           NHRLKLS+FHHRS S+SKP RP EYP+E LC IS SLMADPVVVSSG TF+RLC+QVCQ+
Sbjct: 6   NHRLKLSIFHHRSSSDSKPERPVEYPREYLCEISKSLMADPVVVSSGQTFERLCIQVCQE 65

Query: 61  LGFLPKLEDGSTPDFSSVITNRNVRFTILKWCDNNGIEHPREPDYRSIELIVRQLMEKER 120
           LGF PKLEDGS  DF+SVITNRN+RFTILKWCD+NGIEHPR P Y SIEL+VRQLMEKER
Sbjct: 66  LGFSPKLEDGSRLDFTSVITNRNMRFTILKWCDDNGIEHPRPPLYTSIELVVRQLMEKER 125

Query: 121 RENRFEVSDSELLRGVADKPQGVLNHATTEVGLR--RLHLNSPPQGEEIVRESTLLPFKT 180
           +ENRFE SDSELLRGVADKP GV  HATTEVG R  R  LNSPP+ EEIVREST+LPFKT
Sbjct: 126 QENRFEASDSELLRGVADKPAGVAVHATTEVGFRVNRFQLNSPPETEEIVRESTILPFKT 185

Query: 181 QPSCYAS-SSSNESFEKVPNHRGESNS--PNSGEESRLISKFTSAEPFEQEDGVVSLRRM 240
           QPSCYA+ SSS+ SF+     RG S+S  PNS EESRLI KF S +  EQ++GV+SLR++
Sbjct: 186 QPSCYAAHSSSSSSFD-----RGSSDSYTPNSEEESRLIMKFRSGDECEQQEGVISLRKL 245

Query: 241 TKSDESIRVSLCTKELLAALLPLVSSRSSNVQINAVASVVNLSLDKSNKLKIVRAGFVPP 300
           TKS+ES R S+CTKE LAALLPLVSSRS+N+QINAVASVVNLSL+KSNKLKI+RAGFVP 
Sbjct: 246 TKSNESTRASICTKEFLAALLPLVSSRSTNIQINAVASVVNLSLEKSNKLKILRAGFVPS 305

Query: 301 LIDVLDSGFDEPQEHAAGALFSLSLDDENKMAIGILGALPVLMNTVRSDSERTRHDSALC 360
           LIDVLDSG+ E QEHAAGALFSLSL+DENKMAIGILGALPVLMNT+RS+SERTR+DSALC
Sbjct: 306 LIDVLDSGYAESQEHAAGALFSLSLEDENKMAIGILGALPVLMNTLRSESERTRNDSALC 365

Query: 361 LYHLTLNPSNRVKLVKLGAVPILLSLAKLEDSASRILLILCNIAVSVDGRSAMLDANAVG 420
           LYHLTLNPSNRVKLVKLGAVPILLSL +++ SASRILLILCN+AV  DGRSAMLDANAV 
Sbjct: 366 LYHLTLNPSNRVKLVKLGAVPILLSLTRIDGSASRILLILCNLAVCADGRSAMLDANAVD 425

Query: 421 CLVGILKEKTMDSESTRENCVVALHALSQGGFRFRGLAKEAGAVEVLREIEERGSERARE 480
           CLVG+L+EK MDSESTRENCVVALHALSQGGFRF+GLAK AGAVEVLRE+EERGSERARE
Sbjct: 426 CLVGMLREKEMDSESTRENCVVALHALSQGGFRFKGLAKAAGAVEVLREVEERGSERARE 485

Query: 481 KAKRILQMMR 486
           KAKRIL+MMR
Sbjct: 486 KAKRILEMMR 490

BLAST of MS020048 vs. NCBI nr
Match: XP_023519818.1 (U-box domain-containing protein 38-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 747.3 bits (1928), Expect = 8.4e-212
Identity = 395/490 (80.61%), Postives = 437/490 (89.18%), Query Frame = 0

Query: 1   NHRLKLSLFHHRSPSESKPGRPKEYPQECLCPISNSLMADPVVVSSGHTFDRLCVQVCQD 60
           NHRLKLS+FHHRS S+SKP RP EYP+E LC IS SLMADPVVVSSG TF+RLCVQVCQ+
Sbjct: 6   NHRLKLSIFHHRSSSDSKPERPVEYPREYLCEISKSLMADPVVVSSGQTFERLCVQVCQE 65

Query: 61  LGFLPKLEDGSTPDFSSVITNRNVRFTILKWCDNNGIEHPREPDYRSIELIVRQLMEKER 120
           LGF PKLEDGS  D +SVITNRN+RFTILKWCD+NGIEHPR P Y SIEL+VRQLME+ER
Sbjct: 66  LGFSPKLEDGSRLDCTSVITNRNMRFTILKWCDDNGIEHPRPPQYTSIELVVRQLMERER 125

Query: 121 RENRFEVSDSELLRGVADKPQGVLNHATTEVGLR--RLHLNSPPQGEEIVRESTLLPFKT 180
           +ENRFE SDSELLRGVADKP GV  HATTEVG R  R  LNSPP+ EEIVREST+LPFKT
Sbjct: 126 QENRFETSDSELLRGVADKPPGVAVHATTEVGFRVNRFQLNSPPETEEIVRESTILPFKT 185

Query: 181 QPSCYAS-SSSNESFEKVPNHRGESNS--PNSGEESRLISKFTSAEPFEQEDGVVSLRRM 240
           QPSCYA+ SSS+ SF+     RG S+S  PNS EES LI KF S + FEQ++GV+SLR++
Sbjct: 186 QPSCYAAHSSSSSSFD-----RGSSDSYTPNSEEESHLIMKFRSGDEFEQQEGVISLRKL 245

Query: 241 TKSDESIRVSLCTKELLAALLPLVSSRSSNVQINAVASVVNLSLDKSNKLKIVRAGFVPP 300
           TKS+ESIR SLCTKE LAALLPLVSSRS+N+QINAVASVVNLSL+KSNKLKI+RAGFVP 
Sbjct: 246 TKSNESIRASLCTKEFLAALLPLVSSRSTNIQINAVASVVNLSLEKSNKLKILRAGFVPS 305

Query: 301 LIDVLDSGFDEPQEHAAGALFSLSLDDENKMAIGILGALPVLMNTVRSDSERTRHDSALC 360
           LIDVLDSG+ E QEHAAGALFSLSL+DENKMAIGILGALPVLMNT+RS+SERTR+DSALC
Sbjct: 306 LIDVLDSGYAESQEHAAGALFSLSLEDENKMAIGILGALPVLMNTLRSESERTRNDSALC 365

Query: 361 LYHLTLNPSNRVKLVKLGAVPILLSLAKLEDSASRILLILCNIAVSVDGRSAMLDANAVG 420
           LYHLTLNPSNRVKLVKLGAVPILLS  ++E SASRILLILCN+AV  DGRSAMLDANAV 
Sbjct: 366 LYHLTLNPSNRVKLVKLGAVPILLSSTRIEGSASRILLILCNLAVCADGRSAMLDANAVD 425

Query: 421 CLVGILKEKTMDSESTRENCVVALHALSQGGFRFRGLAKEAGAVEVLREIEERGSERARE 480
           CLVG+L+EK +DSESTRENCVVALHALSQGGFRF+GLAK AGAVEVLRE+EERGSERARE
Sbjct: 426 CLVGMLREKEIDSESTRENCVVALHALSQGGFRFKGLAKAAGAVEVLREVEERGSERARE 485

Query: 481 KAKRILQMMR 486
           KAKRIL+MMR
Sbjct: 486 KAKRILEMMR 490

BLAST of MS020048 vs. NCBI nr
Match: XP_023001501.1 (U-box domain-containing protein 38-like [Cucurbita maxima])

HSP 1 Score: 742.3 bits (1915), Expect = 2.7e-210
Identity = 389/488 (79.71%), Postives = 435/488 (89.14%), Query Frame = 0

Query: 1   NHRLKLSLFHHRSPSESKPGRPKEYPQECLCPISNSLMADPVVVSSGHTFDRLCVQVCQD 60
           NHRLKLS+FHHRS  +SKP RP EYP+E LC IS SLMADPVVVSSG TF+RLCVQVCQ+
Sbjct: 6   NHRLKLSIFHHRSSLDSKPERPVEYPREYLCEISKSLMADPVVVSSGQTFERLCVQVCQE 65

Query: 61  LGFLPKLEDGSTPDFSSVITNRNVRFTILKWCDNNGIEHPREPDYRSIELIVRQLMEKER 120
           LGF PKLEDGS  DF+SVITNRN+RFTILKWCD+NGIEHPR P Y  IEL+VRQLMEKER
Sbjct: 66  LGFSPKLEDGSRLDFTSVITNRNMRFTILKWCDDNGIEHPRPPQYTFIELVVRQLMEKER 125

Query: 121 RENRFEVSDSELLRGVADKPQGVLNHATTEVGLR--RLHLNSPPQGEEIVRESTLLPFKT 180
           +ENRFE SDSELLRGVADKP GV  HATTEVG R  R  LNSPP+ EEIVREST+LPFKT
Sbjct: 126 QENRFEASDSELLRGVADKPPGVAVHATTEVGFRVNRFQLNSPPEREEIVRESTILPFKT 185

Query: 181 QPSCYAS-SSSNESFEKVPNHRGESNSPNSGEESRLISKFTSAEPFEQEDGVVSLRRMTK 240
           QPSCYA+ SSS+ +F++      +S +PNS EESRLI KF S + FEQ++GV+SLR++TK
Sbjct: 186 QPSCYAAHSSSSSNFDR---GCSDSYTPNSEEESRLIMKFRSGDEFEQQEGVISLRKLTK 245

Query: 241 SDESIRVSLCTKELLAALLPLVSSRSSNVQINAVASVVNLSLDKSNKLKIVRAGFVPPLI 300
           S+ES RVS+CTKE LAAL PLVSSRS+N+QINAVASVVNLSL+KSNKLKI+RAGFVP LI
Sbjct: 246 SNESTRVSICTKEFLAALHPLVSSRSTNIQINAVASVVNLSLEKSNKLKILRAGFVPSLI 305

Query: 301 DVLDSGFDEPQEHAAGALFSLSLDDENKMAIGILGALPVLMNTVRSDSERTRHDSALCLY 360
           DVLDSG+ E QEHAAGALFSLSL+DENKMAIGILGALPVLMNT+RS+SERTR+DSALCLY
Sbjct: 306 DVLDSGYAESQEHAAGALFSLSLEDENKMAIGILGALPVLMNTLRSESERTRNDSALCLY 365

Query: 361 HLTLNPSNRVKLVKLGAVPILLSLAKLEDSASRILLILCNIAVSVDGRSAMLDANAVGCL 420
           HLTLNPSNRVKLVKLGAVPILLSL ++E SASRILL+LCN++V  DGRSAMLDANAV CL
Sbjct: 366 HLTLNPSNRVKLVKLGAVPILLSLTRIEGSASRILLVLCNLSVCADGRSAMLDANAVDCL 425

Query: 421 VGILKEKTMDSESTRENCVVALHALSQGGFRFRGLAKEAGAVEVLREIEERGSERAREKA 480
           VG+L+EK M SESTRENCVVALHALSQGGFRF+GLAK AGAVEVLRE+EERGSERAREKA
Sbjct: 426 VGMLREKEMGSESTRENCVVALHALSQGGFRFKGLAKAAGAVEVLREVEERGSERAREKA 485

Query: 481 KRILQMMR 486
           KRIL+MMR
Sbjct: 486 KRILEMMR 490

BLAST of MS020048 vs. NCBI nr
Match: XP_004152547.1 (U-box domain-containing protein 38 [Cucumis sativus] >KGN64288.1 hypothetical protein Csa_013227 [Cucumis sativus])

HSP 1 Score: 741.9 bits (1914), Expect = 3.5e-210
Identity = 396/493 (80.32%), Postives = 433/493 (87.83%), Query Frame = 0

Query: 1   NHRLKLSLFHHRSPSESKPGRPKEYPQECLCPISNSLMADPVVVSSGHTFDRLCVQVCQD 60
           NHRLKLS+FHHRS S+ KP RPK+ PQE LCPIS SLMADPVVVSSG TF+RL V+VCQD
Sbjct: 6   NHRLKLSIFHHRSSSDPKPQRPKDCPQEYLCPISKSLMADPVVVSSGQTFERLSVEVCQD 65

Query: 61  LGFLPKL-EDGSTPDFSSVITNRNVRFTILKWCDNNGIEHPREPDYRSIELIVRQLMEKE 120
           LGF P+L ED S  DFSSVITNRN+R TILKWCDNNGIEHP+ P Y SIEL+VRQLM+KE
Sbjct: 66  LGFSPRLEEDDSRSDFSSVITNRNIRSTILKWCDNNGIEHPQPPSYTSIELVVRQLMQKE 125

Query: 121 RRENRFEVSDSELLRGVADKPQGVLNHATTEVGLR--RLHLNSPPQGEEIVRESTLLPFK 180
            +ENRFEVSDS L+RGVADKP GV  HATTEVGLR  R  LNSP Q EE++RESTLLPFK
Sbjct: 126 EQENRFEVSDSALIRGVADKPTGVAVHATTEVGLRLNRFQLNSPEQTEEVIRESTLLPFK 185

Query: 181 TQPSCYAS---SSSNESFEKVPNHRGESNS--PNSGEESRLISKFTSAEPFEQEDGVVSL 240
           TQPS YA+   SSS+   ++  NHRG S+S  PN  EESRLI KF S + FEQ +GV+SL
Sbjct: 186 TQPSSYATNSPSSSHGGIDRDSNHRGYSDSYAPNLEEESRLILKFRSNDEFEQREGVISL 245

Query: 241 RRMTKSDESIRVSLCTKELLAALLPLVSSRSSNVQINAVASVVNLSLDKSNKLKIVRAGF 300
           R++TKS+ESIR SLCTKE LAALLPL+ SRS+NVQINAVASVVNLSL+K+NKLKIVRAGF
Sbjct: 246 RKLTKSNESIRASLCTKEFLAALLPLILSRSTNVQINAVASVVNLSLEKANKLKIVRAGF 305

Query: 301 VPPLIDVLDSGFDEPQEHAAGALFSLSLDDENKMAIGILGALPVLMNTVRSDSERTRHDS 360
           VPPLIDVLD G  E QEHAAGALFSLSLDDENKMAIGILGALPVLMNT+RSDSERTR+DS
Sbjct: 306 VPPLIDVLDGGNTESQEHAAGALFSLSLDDENKMAIGILGALPVLMNTLRSDSERTRNDS 365

Query: 361 ALCLYHLTLNPSNRVKLVKLGAVPILLSLAKLEDSASRILLILCNIAVSVDGRSAMLDAN 420
           ALCLYHLTLNPSNRVKLVKLGAVPILLSL ++E   SRI+LILCNIAVSVDGRSAMLDAN
Sbjct: 366 ALCLYHLTLNPSNRVKLVKLGAVPILLSLTRIEGCTSRIVLILCNIAVSVDGRSAMLDAN 425

Query: 421 AVGCLVGILKEKTMDSESTRENCVVALHALSQGGFRFRGLAKEAGAVEVLREIEERGSER 480
           AV CLVG+LKEK MDSESTRENCVVAL+ALSQGGFRFRGLAKEAGAVEVLRE+EERGSER
Sbjct: 426 AVSCLVGLLKEKEMDSESTRENCVVALYALSQGGFRFRGLAKEAGAVEVLREVEERGSER 485

Query: 481 AREKAKRILQMMR 486
           AREKAKRILQMMR
Sbjct: 486 AREKAKRILQMMR 498

BLAST of MS020048 vs. ExPASy Swiss-Prot
Match: Q9FJP6 (U-box domain-containing protein 38 OS=Arabidopsis thaliana OX=3702 GN=PUB38 PE=1 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 1.6e-128
Identity = 269/508 (52.95%), Postives = 354/508 (69.69%), Query Frame = 0

Query: 1   NHRLKLSLFHHRSPSESKPG-----RPKEYPQECLCPISNSLMADPVVVSSGHTFDRLCV 60
           N RL+ + F HRS S +        + ++ P E LCPIS S+M+DPVVVSSG TF+R+CV
Sbjct: 4   NGRLRWNPFSHRSSSSTSSSSRQQQQEQQPPVEFLCPISKSVMSDPVVVSSGQTFERVCV 63

Query: 61  QVCQDLGFLPKLEDG--STPDFSSVITNRNVRFTILKWCDNNGIEHPREPDYRSIELIVR 120
           QVC+DL F+PKL D   S PDFS++I N N++ TI  WCD  G+  P+ PDY ++E I+R
Sbjct: 64  QVCRDLNFIPKLNDDEESLPDFSNIIPNLNMKSTIDTWCDTVGVSRPQPPDYSTVERILR 123

Query: 121 QLMEKERRENRFEVSDSELLRGVADKPQGVLNHATTEVGLRRLHLNSPPQGEE--IVRES 180
           Q M     E R  VS+ ELLR VA +   +++HA +E+  RR   NS    +E  IV  S
Sbjct: 124 QQMPPPDVEIR--VSEQELLRAVAHRAPMIIHHADSELMGRRDFNNSTTSSDESVIVAHS 183

Query: 181 --TLLPFKTQPSCY--ASSSSNESFEKVPNHRGESNSPNSG--EESRLISKFTSAEPFEQ 240
             T LP  T+P+C+  + SSS+   E + +H   SNS ++   E+  + +K  S+E F+Q
Sbjct: 184 PFTPLPLTTRPACFSPSPSSSSSEIETLTHHTFFSNSTSTATEEDEVIYNKLKSSEIFDQ 243

Query: 241 EDGVVSLRRMTKSDESIRVSLCTKELLAALLPLVSSRSSNVQINAVASVVNLSLDKSNKL 300
           E G++ +R+MT++++  RVSLC+  +L+ L  ++ SR S VQ NA+AS+VNLSLDK NKL
Sbjct: 244 EQGLIMMRKMTRTNDEARVSLCSPRILSLLKNMIVSRYSLVQTNALASLVNLSLDKKNKL 303

Query: 301 KIVRAGFVPPLIDVLDSGFDEPQEHAAGALFSLSLDDENKMAIGILGALPVLMNTVR-SD 360
            IVR GFVP LIDVL SG  E QEHAAG +FSLSL+D+NKM IG+LGAL  L++ +R ++
Sbjct: 304 TIVRLGFVPILIDVLKSGSREAQEHAAGTIFSLSLEDDNKMPIGVLGALQPLLHALRAAE 363

Query: 361 SERTRHDSALCLYHLTLNPSNRVKLVKLGAVPILLSLAKLEDSASRILLILCNIAVSVDG 420
           S+RTRHDSAL LYHLTLN +NR KLV+LGAVP L S+ +  +SASR LL++CN+A   +G
Sbjct: 364 SDRTRHDSALALYHLTLNQTNRSKLVRLGAVPALFSMVRSGESASRALLVICNLACCSEG 423

Query: 421 RSAMLDANAVGCLVGILKE-------KTMDSESTRENCVVALHALSQGGFRFRGLAKEAG 480
           RSAMLDANAV  LVG L+E       +   S S RENCV AL ALS    RF+GLAKEA 
Sbjct: 424 RSAMLDANAVAILVGKLREEWTEEPTEARSSSSARENCVAALFALSHESLRFKGLAKEAR 483

Query: 481 AVEVLREIEERGSERAREKAKRILQMMR 486
           AVEVL+E+EERG+ERAREKAK+ILQ+MR
Sbjct: 484 AVEVLKEVEERGTERAREKAKKILQLMR 509

BLAST of MS020048 vs. ExPASy Swiss-Prot
Match: Q0WUF6 (U-box domain-containing protein 41 OS=Arabidopsis thaliana OX=3702 GN=PUB41 PE=2 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 5.1e-111
Identity = 242/503 (48.11%), Postives = 317/503 (63.02%), Query Frame = 0

Query: 9   FHHRSPSESKPGRPK----EYPQECLCPISNSLMADPVVVSSGHTFDRLCVQVCQDLGFL 68
           FH RS S +    P+    E P E LCPI+  LM+DPVVVSSG TF+RL VQVC++LG++
Sbjct: 11  FHQRSSSATTTTLPQHKHDETPPEFLCPITGFLMSDPVVVSSGQTFERLSVQVCRNLGYI 70

Query: 69  PKLEDGSTPDFSSVITNRNVRFTILKWCDNNGIEHPREPDYRSIELIVRQLMEKERRENR 128
           P L DG+ PD S+VI N  ++ TI  WCD   ++HPR PD   +E +VR  M+K+   + 
Sbjct: 71  PDLLDGTRPDLSTVIPNLAMKSTIFSWCDRQKVDHPRPPDAAYVEGVVRARMDKDPNPSP 130

Query: 129 FEV-------SDSELLRGVADKPQGVLNHATTEVGLRRLHLNSPPQGEEIVR--ESTLLP 188
            +         + E+L  V +      +     +  R  +  SP    E V   +S+  P
Sbjct: 131 GQSPGPGDKDPEPEILPPVEENSPSDYDAVMEAIRARSKNSMSPTTSLESVTIGQSSYHP 190

Query: 189 FKTQPSCYASSSSNES---------FEKVPNHRG--ESNSPNSGEESRLISKFTSAEPFE 248
            +      +S++S+ S         F    +      S+SP S EE  + +K    + F+
Sbjct: 191 VRAVSMFSSSTTSSSSGVFAGADSPFRNAMSFSSTDHSSSPMSPEEEEIFNKLRGTDIFD 250

Query: 249 QEDGVVSLRRMTKSDESIRVSLCTKELLAALLPLVSSRSSNVQINAVASVVNLSLDKSNK 308
            E G++ LR+MT+S E +RVSLCT  +L+ L  L+ SR + VQ NA ASVVNLSL+K NK
Sbjct: 251 HEQGLILLRKMTRSSEDLRVSLCTDRILSFLRSLLVSRYNLVQTNAAASVVNLSLEKQNK 310

Query: 309 LKIVRAGFVPPLIDVLDSGFDEPQEHAAGALFSLSLDDENKMAIGILGALPVLMNTVR-S 368
           +KIVR+GFVP LIDVL SG  E QEH AGALFSL+L+DENKM IG+LGA+  L++ +R S
Sbjct: 311 VKIVRSGFVPLLIDVLKSGTTEAQEHVAGALFSLALEDENKMVIGVLGAVEPLLHALRSS 370

Query: 369 DSERTRHDSALCLYHLTLNPSNRVKLVKLGAVPILLSLAKLEDSASRILLILCNIAVSVD 428
           +SER R D+AL LYHL+L PSNR +LV+ GAVP LLS+ +  DS SRILL+LCN+A   D
Sbjct: 371 ESERARQDAALALYHLSLIPSNRTRLVRAGAVPTLLSMVRSGDSTSRILLVLCNLAACPD 430

Query: 429 GRSAMLDANAVGCLVGILKE-KTMDSESTRENCVVALHALSQGGFRFRGLAKEAGAVEVL 486
           G+ AMLD NAV  LVG L+E    DSE+ RENCV  L  L QG  RFRGLA EAGA EVL
Sbjct: 431 GKGAMLDGNAVAILVGKLREVGGGDSEAARENCVAVLLTLCQGNLRFRGLASEAGAEEVL 490

BLAST of MS020048 vs. ExPASy Swiss-Prot
Match: Q9STT1 (U-box domain-containing protein 39 OS=Arabidopsis thaliana OX=3702 GN=PUB39 PE=1 SV=2)

HSP 1 Score: 399.4 bits (1025), Expect = 5.7e-110
Identity = 236/492 (47.97%), Postives = 316/492 (64.23%), Query Frame = 0

Query: 7   SLFHHRSPSES---KPGRPKEYPQECLCPISNSLMADPVVVSSGHTFDRLCVQVCQDLGF 66
           S  HHRS S +   +   P E P E LCPI+  LM+DPVVV+SG TF+R+ VQVC++L F
Sbjct: 11  SFHHHRSASATTMPQHNSPGETPTEFLCPITGFLMSDPVVVASGQTFERISVQVCRNLSF 70

Query: 67  LPKLEDGSTPDFSSVITNRNVRFTILKWCDNNGIEHPREPDYRSIELIVRQLMEKERREN 126
            PKL DG+ PD S+VI N  ++ TIL WCD N +EHPR PDY  +E +VR  M+     +
Sbjct: 71  APKLHDGTQPDLSTVIPNLAMKSTILSWCDRNKMEHPRPPDYAYVEGVVRTRMDSLPPGS 130

Query: 127 RFEVSDSELLRGVADKPQGVLNHATTEVGLRRLHLNSPPQGEEIVRESTLLPF-KTQPSC 186
              ++ SE+L  VA+      ++ +    +R     S       +  +T LP  +T+P  
Sbjct: 131 GHRIAKSEILPPVAENSNSNSDYDSVMGAIRSRSRTS-------LSSTTSLPLHQTRPIN 190

Query: 187 YA----SSSSNESFEKVPNHRGESNSPNSGEESRLISKFTSAEPFEQEDGVVSLRRMTKS 246
           ++    SS S   +   P        P S EE  + +K TS +  + E G++ LR+ T+S
Sbjct: 191 HSTRIQSSFSTSDYSSFP--------PMSPEEEEIYNKLTSVDTIDHEQGLIQLRKTTRS 250

Query: 247 DESIRVSLCTKELLAALLPLVSSRSSNVQINAVASVVNLSLDKSNKLKIVRAGFVPPLID 306
           +E+ R+SLCT  +L+ L  L+ SR + VQ NA AS+VNLSL+K NKLKIVR+GFVP LID
Sbjct: 251 NETTRISLCTDRILSLLRSLIVSRYNIVQTNAAASIVNLSLEKPNKLKIVRSGFVPLLID 310

Query: 307 VLDSGFDEPQEHAAGALFSLSLDDENKMAIGILGALPVLMNTVR-SDSERTRHDSALCLY 366
           VL SG  E QEH  GALFSL++++ENKM IG+LGA+  L++ +R S+SER R D+AL LY
Sbjct: 311 VLKSGSTEAQEHVIGALFSLAVEEENKMVIGVLGAVEPLLHALRSSESERARQDAALALY 370

Query: 367 HLTLNPSNRVKLVKLGAVPILLSLAKLEDSASRILLILCNIAVSVDGRSAMLDANAVGCL 426
           HL+L P+NR +LVK GAVP++LS+ +  +SASRILL+LCN+A   +G+ AMLD NAV  L
Sbjct: 371 HLSLIPNNRSRLVKAGAVPMMLSMIRSGESASRILLLLCNLAACSEGKGAMLDGNAVSIL 430

Query: 427 VGILKEK--TMDSESTRENCVVALHALSQGGFRFRGLAKEAGAVEVLREI--EERGSERA 486
           VG L+E        + RENCV AL  LS G  RFRGLA EAGA E+L EI   E GS R 
Sbjct: 431 VGKLRESGGAESDAAARENCVGALLTLSVGNMRFRGLASEAGAEEILTEIVESESGSGRL 487

BLAST of MS020048 vs. ExPASy Swiss-Prot
Match: Q9FL17 (U-box domain-containing protein 40 OS=Arabidopsis thaliana OX=3702 GN=PUB40 PE=2 SV=2)

HSP 1 Score: 398.3 bits (1022), Expect = 1.3e-109
Identity = 229/485 (47.22%), Postives = 309/485 (63.71%), Query Frame = 0

Query: 3   RLKLSLFHHRSPSESKPGRPKEYPQECLCPISNSLMADPVVVSSGHTFDRLCVQVCQDLG 62
           R  LS     S S +      E P E LCPIS SLMADP++VSSGH+++R CV  C+ LG
Sbjct: 36  RTSLSRSSSSSSSNNNSPTKTEIPAEFLCPISGSLMADPIIVSSGHSYERACVIACKTLG 95

Query: 63  FLPKLEDGSTPDFSSVITNRNVRFTILKWCDNNGIEHPREPDYRSIELIVRQLMEKERRE 122
           F P       PDFS+VI N  ++  I  WC+      P+  +  + E ++  LMEK+ + 
Sbjct: 96  FTPT----PPPDFSTVIPNLALKSAIHSWCERRCFPPPKPLNSAAAEKLILALMEKKPQR 155

Query: 123 NRFEVSDSELLRGVADKPQGVLNHATTEVGLRRLHLNSPPQGEEIVRESTLLPFKTQPSC 182
            +  VS+ EL++ + DKP   LNHA TE+  R  + NS    E I   S  L   T+PSC
Sbjct: 156 RKVSVSEKELIQAIRDKPSVRLNHAATELDRRPNYFNS-SSDESIASSSRTLQLTTKPSC 215

Query: 183 YASSSSNESFEKVPNHRGESNSPN-SGEESRLISKFTSAEPFEQEDGVVSLRRMTKSDES 242
           ++S SS E          ES  PN + EE  L++K  S    E E+ ++S+RR+T+ DES
Sbjct: 216 FSSPSSGEI---------ESLEPNLTPEEEALLTKLKSNRISEIEEALISIRRITRIDES 275

Query: 243 IRVSLCTKELLAALLPLVSSRSSNVQINAVASVVNLSLDKSNKLKIVRAGFVPPLIDVLD 302
            R+SLCT  +++AL  L+ SR + VQ+N  A +VNLSL+KSNK+KIVR+G VPPLIDVL 
Sbjct: 276 SRISLCTTRVISALKSLIVSRYATVQVNVTAVLVNLSLEKSNKVKIVRSGIVPPLIDVLK 335

Query: 303 SGFDEPQEHAAGALFSLSLDDENKMAIGILGALPVLMNTVRSDSERTRHDSALCLYHLTL 362
            G  E QEH+AG +FSL+L+DENK AIG+LG L  L++ +R  +E TRHDSAL LYHL+L
Sbjct: 336 CGSVEAQEHSAGVIFSLALEDENKTAIGVLGGLEPLLHLIRVGTELTRHDSALALYHLSL 395

Query: 363 NPSNRVKLVKLGAVPILLSLAKLEDSASRILLILCNIAVSVDGRSAMLDANAVGCLVGIL 422
             SNR KLVKLGAV +LL +  L     R+LLILCN+A     R A+LD+  V C+VG+L
Sbjct: 396 VQSNRGKLVKLGAVQMLLGMVSLGQMIGRVLLILCNMASCPVSRPALLDSGGVECMVGVL 455

Query: 423 KEKTMDSESTRENCVVALHALS-QGGFRFRGLAKEAGAVEVLREIEERGSERAREKAKRI 482
           +     +ESTRE+CV  L+ LS  GG RF+GLA  A AVE L ++E  G ERA++KA+R+
Sbjct: 456 RRDREVNESTRESCVAVLYGLSHDGGLRFKGLAMAANAVEELVKVERSGRERAKQKARRV 506

Query: 483 LQMMR 486
           L+++R
Sbjct: 516 LEVLR 506

BLAST of MS020048 vs. ExPASy Swiss-Prot
Match: Q9ZV31 (U-box domain-containing protein 12 OS=Arabidopsis thaliana OX=3702 GN=PUB12 PE=2 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 2.0e-38
Identity = 137/462 (29.65%), Postives = 215/462 (46.54%), Query Frame = 0

Query: 26  PQECLCPISNSLMADPVVVSSGHTFDRLCVQVCQDLGFL--PKLEDGSTPDFSSVITNRN 85
           P+E  CPIS  LM DPV+VSSG T++R C++   + G L  PK ++  T D   +  N  
Sbjct: 257 PEEFRCPISLELMTDPVIVSSGQTYERECIKKWLEGGHLTCPKTQETLTSDI--MTPNYV 316

Query: 86  VRFTILKWCDNNGIEHPREPDYRSIELIVRQLMEKERRENRFEVSDSELLRGVADKPQGV 145
           +R  I +WC++NGIE P+ P+                                       
Sbjct: 317 LRSLIAQWCESNGIEPPKRPNI-------------------------------------- 376

Query: 146 LNHATTEVGLRRLHLNSPPQGEEIVRESTLLPFKTQPSCYASSSSNESFEKVPNHRGESN 205
                                             +QPS  ASSSS+             +
Sbjct: 377 ----------------------------------SQPSSKASSSSS----------APDD 436

Query: 206 SPNSGEESRLISKFTSAEPFEQEDGVVSLRRMTKSDESIRVSLCTKELLAALLPLVS-SR 265
             N  EE  L+ K TS +P ++      +R + K +   RV++     +  L+ L++ S 
Sbjct: 437 EHNKIEE--LLLKLTSQQPEDRRSAAGEIRLLAKQNNHNRVAIAASGAIPLLVNLLTISN 496

Query: 266 SSNVQINAVASVVNLSLDKSNKLKIV-RAGFVPPLIDVLDSGFDEPQEHAAGALFSLSLD 325
            S  Q +AV S++NLS+ + NK KIV  +G VP ++ VL  G  E +E+AA  LFSLS+ 
Sbjct: 497 DSRTQEHAVTSILNLSICQENKGKIVYSSGAVPGIVHVLQKGSMEARENAAATLFSLSVI 556

Query: 326 DENKMAIGILGALPVLMNTVRSDSERTRHDSALCLYHLTLNPSNRVKLVKLGAVPILLSL 385
           DENK+ IG  GA+P L+  +   S+R + D+A  L++L +   N+ K V+ G VP+L+ L
Sbjct: 557 DENKVTIGAAGAIPPLVTLLSEGSQRGKKDAATALFNLCIFQGNKGKAVRAGLVPVLMRL 616

Query: 386 AKLEDS--ASRILLILCNIAVSVDGRSAMLDANAVGCLVGILKEKTMDSESTRENCVVAL 445
               +S      L IL  ++   DG+S +  A+AV  LV  ++     S   +EN    L
Sbjct: 617 LTEPESGMVDESLSILAILSSHPDGKSEVGAADAVPVLVDFIRS---GSPRNKENSAAVL 629

Query: 446 HALSQGGFRFRGLAKEAGAVEVLREIEERGSERAREKAKRIL 482
             L     +    A++ G +++L E+ E G++R + KA ++L
Sbjct: 677 VHLCSWNQQHLIEAQKLGIMDLLIEMAENGTDRGKRKAAQLL 629

BLAST of MS020048 vs. ExPASy TrEMBL
Match: A0A6J1E734 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111431320 PE=4 SV=1)

HSP 1 Score: 748.4 bits (1931), Expect = 1.8e-212
Identity = 395/490 (80.61%), Postives = 438/490 (89.39%), Query Frame = 0

Query: 1   NHRLKLSLFHHRSPSESKPGRPKEYPQECLCPISNSLMADPVVVSSGHTFDRLCVQVCQD 60
           NHRLKLS+FHHRS S+SKP RP EYP+E LC IS SLMADPVVVSSG TF+RLC+QVCQ+
Sbjct: 6   NHRLKLSIFHHRSSSDSKPERPVEYPREYLCEISKSLMADPVVVSSGQTFERLCIQVCQE 65

Query: 61  LGFLPKLEDGSTPDFSSVITNRNVRFTILKWCDNNGIEHPREPDYRSIELIVRQLMEKER 120
           LGF PKLEDGS  DF+SVITNRN+RFTILKWCD+NGIEHPR P Y SIEL+VRQLMEKER
Sbjct: 66  LGFSPKLEDGSRLDFTSVITNRNMRFTILKWCDDNGIEHPRPPLYTSIELVVRQLMEKER 125

Query: 121 RENRFEVSDSELLRGVADKPQGVLNHATTEVGLR--RLHLNSPPQGEEIVRESTLLPFKT 180
           +ENRFE SDSELLRGVADKP GV  HATTEVG R  R  LNSPP+ EEIVREST+LPFKT
Sbjct: 126 QENRFEASDSELLRGVADKPAGVAVHATTEVGFRVNRFQLNSPPETEEIVRESTILPFKT 185

Query: 181 QPSCYAS-SSSNESFEKVPNHRGESNS--PNSGEESRLISKFTSAEPFEQEDGVVSLRRM 240
           QPSCYA+ SSS+ SF+     RG S+S  PNS EESRLI KF S +  EQ++GV+SLR++
Sbjct: 186 QPSCYAAHSSSSSSFD-----RGSSDSYTPNSEEESRLIMKFRSGDECEQQEGVISLRKL 245

Query: 241 TKSDESIRVSLCTKELLAALLPLVSSRSSNVQINAVASVVNLSLDKSNKLKIVRAGFVPP 300
           TKS+ES R S+CTKE LAALLPLVSSRS+N+QINAVASVVNLSL+KSNKLKI+RAGFVP 
Sbjct: 246 TKSNESTRASICTKEFLAALLPLVSSRSTNIQINAVASVVNLSLEKSNKLKILRAGFVPS 305

Query: 301 LIDVLDSGFDEPQEHAAGALFSLSLDDENKMAIGILGALPVLMNTVRSDSERTRHDSALC 360
           LIDVLDSG+ E QEHAAGALFSLSL+DENKMAIGILGALPVLMNT+RS+SERTR+DSALC
Sbjct: 306 LIDVLDSGYAESQEHAAGALFSLSLEDENKMAIGILGALPVLMNTLRSESERTRNDSALC 365

Query: 361 LYHLTLNPSNRVKLVKLGAVPILLSLAKLEDSASRILLILCNIAVSVDGRSAMLDANAVG 420
           LYHLTLNPSNRVKLVKLGAVPILLSL +++ SASRILLILCN+AV  DGRSAMLDANAV 
Sbjct: 366 LYHLTLNPSNRVKLVKLGAVPILLSLTRIDGSASRILLILCNLAVCADGRSAMLDANAVD 425

Query: 421 CLVGILKEKTMDSESTRENCVVALHALSQGGFRFRGLAKEAGAVEVLREIEERGSERARE 480
           CLVG+L+EK MDSESTRENCVVALHALSQGGFRF+GLAK AGAVEVLRE+EERGSERARE
Sbjct: 426 CLVGMLREKEMDSESTRENCVVALHALSQGGFRFKGLAKAAGAVEVLREVEERGSERARE 485

Query: 481 KAKRILQMMR 486
           KAKRIL+MMR
Sbjct: 486 KAKRILEMMR 490

BLAST of MS020048 vs. ExPASy TrEMBL
Match: A0A6J1KIT1 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111495624 PE=4 SV=1)

HSP 1 Score: 742.3 bits (1915), Expect = 1.3e-210
Identity = 389/488 (79.71%), Postives = 435/488 (89.14%), Query Frame = 0

Query: 1   NHRLKLSLFHHRSPSESKPGRPKEYPQECLCPISNSLMADPVVVSSGHTFDRLCVQVCQD 60
           NHRLKLS+FHHRS  +SKP RP EYP+E LC IS SLMADPVVVSSG TF+RLCVQVCQ+
Sbjct: 6   NHRLKLSIFHHRSSLDSKPERPVEYPREYLCEISKSLMADPVVVSSGQTFERLCVQVCQE 65

Query: 61  LGFLPKLEDGSTPDFSSVITNRNVRFTILKWCDNNGIEHPREPDYRSIELIVRQLMEKER 120
           LGF PKLEDGS  DF+SVITNRN+RFTILKWCD+NGIEHPR P Y  IEL+VRQLMEKER
Sbjct: 66  LGFSPKLEDGSRLDFTSVITNRNMRFTILKWCDDNGIEHPRPPQYTFIELVVRQLMEKER 125

Query: 121 RENRFEVSDSELLRGVADKPQGVLNHATTEVGLR--RLHLNSPPQGEEIVRESTLLPFKT 180
           +ENRFE SDSELLRGVADKP GV  HATTEVG R  R  LNSPP+ EEIVREST+LPFKT
Sbjct: 126 QENRFEASDSELLRGVADKPPGVAVHATTEVGFRVNRFQLNSPPEREEIVRESTILPFKT 185

Query: 181 QPSCYAS-SSSNESFEKVPNHRGESNSPNSGEESRLISKFTSAEPFEQEDGVVSLRRMTK 240
           QPSCYA+ SSS+ +F++      +S +PNS EESRLI KF S + FEQ++GV+SLR++TK
Sbjct: 186 QPSCYAAHSSSSSNFDR---GCSDSYTPNSEEESRLIMKFRSGDEFEQQEGVISLRKLTK 245

Query: 241 SDESIRVSLCTKELLAALLPLVSSRSSNVQINAVASVVNLSLDKSNKLKIVRAGFVPPLI 300
           S+ES RVS+CTKE LAAL PLVSSRS+N+QINAVASVVNLSL+KSNKLKI+RAGFVP LI
Sbjct: 246 SNESTRVSICTKEFLAALHPLVSSRSTNIQINAVASVVNLSLEKSNKLKILRAGFVPSLI 305

Query: 301 DVLDSGFDEPQEHAAGALFSLSLDDENKMAIGILGALPVLMNTVRSDSERTRHDSALCLY 360
           DVLDSG+ E QEHAAGALFSLSL+DENKMAIGILGALPVLMNT+RS+SERTR+DSALCLY
Sbjct: 306 DVLDSGYAESQEHAAGALFSLSLEDENKMAIGILGALPVLMNTLRSESERTRNDSALCLY 365

Query: 361 HLTLNPSNRVKLVKLGAVPILLSLAKLEDSASRILLILCNIAVSVDGRSAMLDANAVGCL 420
           HLTLNPSNRVKLVKLGAVPILLSL ++E SASRILL+LCN++V  DGRSAMLDANAV CL
Sbjct: 366 HLTLNPSNRVKLVKLGAVPILLSLTRIEGSASRILLVLCNLSVCADGRSAMLDANAVDCL 425

Query: 421 VGILKEKTMDSESTRENCVVALHALSQGGFRFRGLAKEAGAVEVLREIEERGSERAREKA 480
           VG+L+EK M SESTRENCVVALHALSQGGFRF+GLAK AGAVEVLRE+EERGSERAREKA
Sbjct: 426 VGMLREKEMGSESTRENCVVALHALSQGGFRFKGLAKAAGAVEVLREVEERGSERAREKA 485

Query: 481 KRILQMMR 486
           KRIL+MMR
Sbjct: 486 KRILEMMR 490

BLAST of MS020048 vs. ExPASy TrEMBL
Match: A0A0A0LR64 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_1G045860 PE=4 SV=1)

HSP 1 Score: 741.9 bits (1914), Expect = 1.7e-210
Identity = 396/493 (80.32%), Postives = 433/493 (87.83%), Query Frame = 0

Query: 1   NHRLKLSLFHHRSPSESKPGRPKEYPQECLCPISNSLMADPVVVSSGHTFDRLCVQVCQD 60
           NHRLKLS+FHHRS S+ KP RPK+ PQE LCPIS SLMADPVVVSSG TF+RL V+VCQD
Sbjct: 6   NHRLKLSIFHHRSSSDPKPQRPKDCPQEYLCPISKSLMADPVVVSSGQTFERLSVEVCQD 65

Query: 61  LGFLPKL-EDGSTPDFSSVITNRNVRFTILKWCDNNGIEHPREPDYRSIELIVRQLMEKE 120
           LGF P+L ED S  DFSSVITNRN+R TILKWCDNNGIEHP+ P Y SIEL+VRQLM+KE
Sbjct: 66  LGFSPRLEEDDSRSDFSSVITNRNIRSTILKWCDNNGIEHPQPPSYTSIELVVRQLMQKE 125

Query: 121 RRENRFEVSDSELLRGVADKPQGVLNHATTEVGLR--RLHLNSPPQGEEIVRESTLLPFK 180
            +ENRFEVSDS L+RGVADKP GV  HATTEVGLR  R  LNSP Q EE++RESTLLPFK
Sbjct: 126 EQENRFEVSDSALIRGVADKPTGVAVHATTEVGLRLNRFQLNSPEQTEEVIRESTLLPFK 185

Query: 181 TQPSCYAS---SSSNESFEKVPNHRGESNS--PNSGEESRLISKFTSAEPFEQEDGVVSL 240
           TQPS YA+   SSS+   ++  NHRG S+S  PN  EESRLI KF S + FEQ +GV+SL
Sbjct: 186 TQPSSYATNSPSSSHGGIDRDSNHRGYSDSYAPNLEEESRLILKFRSNDEFEQREGVISL 245

Query: 241 RRMTKSDESIRVSLCTKELLAALLPLVSSRSSNVQINAVASVVNLSLDKSNKLKIVRAGF 300
           R++TKS+ESIR SLCTKE LAALLPL+ SRS+NVQINAVASVVNLSL+K+NKLKIVRAGF
Sbjct: 246 RKLTKSNESIRASLCTKEFLAALLPLILSRSTNVQINAVASVVNLSLEKANKLKIVRAGF 305

Query: 301 VPPLIDVLDSGFDEPQEHAAGALFSLSLDDENKMAIGILGALPVLMNTVRSDSERTRHDS 360
           VPPLIDVLD G  E QEHAAGALFSLSLDDENKMAIGILGALPVLMNT+RSDSERTR+DS
Sbjct: 306 VPPLIDVLDGGNTESQEHAAGALFSLSLDDENKMAIGILGALPVLMNTLRSDSERTRNDS 365

Query: 361 ALCLYHLTLNPSNRVKLVKLGAVPILLSLAKLEDSASRILLILCNIAVSVDGRSAMLDAN 420
           ALCLYHLTLNPSNRVKLVKLGAVPILLSL ++E   SRI+LILCNIAVSVDGRSAMLDAN
Sbjct: 366 ALCLYHLTLNPSNRVKLVKLGAVPILLSLTRIEGCTSRIVLILCNIAVSVDGRSAMLDAN 425

Query: 421 AVGCLVGILKEKTMDSESTRENCVVALHALSQGGFRFRGLAKEAGAVEVLREIEERGSER 480
           AV CLVG+LKEK MDSESTRENCVVAL+ALSQGGFRFRGLAKEAGAVEVLRE+EERGSER
Sbjct: 426 AVSCLVGLLKEKEMDSESTRENCVVALYALSQGGFRFRGLAKEAGAVEVLREVEERGSER 485

Query: 481 AREKAKRILQMMR 486
           AREKAKRILQMMR
Sbjct: 486 AREKAKRILQMMR 498

BLAST of MS020048 vs. ExPASy TrEMBL
Match: A0A5A7ULP5 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G00470 PE=4 SV=1)

HSP 1 Score: 733.4 bits (1892), Expect = 6.1e-208
Identity = 396/493 (80.32%), Postives = 429/493 (87.02%), Query Frame = 0

Query: 1   NHRLKLSLFHHRSPSESKPGRPKEYPQECLCPISNSLMADPVVVSSGHTFDRLCVQVCQD 60
           NHRLKLS+FH RS S+SKP RPK+ PQE LCPIS SLMADPVVVSSG TF+RL V+VCQD
Sbjct: 6   NHRLKLSIFHQRSSSDSKPQRPKDCPQEYLCPISKSLMADPVVVSSGQTFERLSVKVCQD 65

Query: 61  LGFLPKL-EDGSTPDFSSVITNRNVRFTILKWCDNNGIEHPREPDYRSIELIVRQLMEKE 120
           LGF P L ED S PDFSSVITNRN+R TILKWCDNNGIEHP+ P Y SIELIVRQLM+KE
Sbjct: 66  LGFSPMLEEDDSRPDFSSVITNRNIRSTILKWCDNNGIEHPQPPPYTSIELIVRQLMQKE 125

Query: 121 RRENRFEVSDSELLRGVADKPQGVLNHATTEVGLR--RLHLNSPPQGEEIVRESTLLPFK 180
            +ENRFE SDS L+R VADKP GV  HATTEVGLR  R  LNSP Q EE  RESTLLPFK
Sbjct: 126 EQENRFEASDSALIRRVADKPTGVAVHATTEVGLRHNRFQLNSPEQTEETTRESTLLPFK 185

Query: 181 TQPSCY---ASSSSNESFEKVPNHRG--ESNSPNSGEESRLISKFTSAEPFEQEDGVVSL 240
           TQPS Y   ASSSS+  F++  NH G  +S+SPN  EESRLI KF S + FEQ +GV+SL
Sbjct: 186 TQPSSYAANASSSSHGGFDRDSNHGGYSDSDSPNLEEESRLILKFRSNDEFEQREGVISL 245

Query: 241 RRMTKSDESIRVSLCTKELLAALLPLVSSRSSNVQINAVASVVNLSLDKSNKLKIVRAGF 300
           R++TKS+ESIR SLCTKE LAALLPLV SRS NVQINAVASVVNLSL+K+NKLKIVRAGF
Sbjct: 246 RKLTKSNESIRASLCTKEFLAALLPLVLSRSPNVQINAVASVVNLSLEKANKLKIVRAGF 305

Query: 301 VPPLIDVLDSGFDEPQEHAAGALFSLSLDDENKMAIGILGALPVLMNTVRSDSERTRHDS 360
           VP LIDVLD G  E QEHAAGALFSLSLDDENKMAIGILGALPVLMNT+RSDSERTR+DS
Sbjct: 306 VPLLIDVLDGGNTESQEHAAGALFSLSLDDENKMAIGILGALPVLMNTLRSDSERTRNDS 365

Query: 361 ALCLYHLTLNPSNRVKLVKLGAVPILLSLAKLEDSASRILLILCNIAVSVDGRSAMLDAN 420
           ALCLYHLTLNPSNRVKLVKLGAVPILLSL ++E   SRI+LILCNIAVSVDGRSAMLDAN
Sbjct: 366 ALCLYHLTLNPSNRVKLVKLGAVPILLSLTRIEGCTSRIVLILCNIAVSVDGRSAMLDAN 425

Query: 421 AVGCLVGILKEKTMDSESTRENCVVALHALSQGGFRFRGLAKEAGAVEVLREIEERGSER 480
           AVGCLVG+LK+K MDSESTRENCVVAL+ALSQGGFRFRGLAKEAGAVEVLRE+EERG+ER
Sbjct: 426 AVGCLVGLLKDKAMDSESTRENCVVALYALSQGGFRFRGLAKEAGAVEVLREVEERGTER 485

Query: 481 AREKAKRILQMMR 486
           AREKAKRILQMMR
Sbjct: 486 AREKAKRILQMMR 498

BLAST of MS020048 vs. ExPASy TrEMBL
Match: A0A5D3BJ60 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G003420 PE=4 SV=1)

HSP 1 Score: 732.6 bits (1890), Expect = 1.0e-207
Identity = 396/493 (80.32%), Postives = 428/493 (86.82%), Query Frame = 0

Query: 1   NHRLKLSLFHHRSPSESKPGRPKEYPQECLCPISNSLMADPVVVSSGHTFDRLCVQVCQD 60
           NHRLKLS+FH RS S+SKP RPK+ PQE LCPIS SLMADPVVVSSG TF+RL VQVCQD
Sbjct: 6   NHRLKLSIFHQRSSSDSKPQRPKDCPQEYLCPISKSLMADPVVVSSGQTFERLSVQVCQD 65

Query: 61  LGFLPKL-EDGSTPDFSSVITNRNVRFTILKWCDNNGIEHPREPDYRSIELIVRQLMEKE 120
           LGF P L ED S PDFSSVI NRN+R TILKWCDNNGIEHP+ P Y SIELIVRQLM+KE
Sbjct: 66  LGFSPMLEEDDSRPDFSSVIINRNIRSTILKWCDNNGIEHPQPPPYTSIELIVRQLMQKE 125

Query: 121 RRENRFEVSDSELLRGVADKPQGVLNHATTEVGLR--RLHLNSPPQGEEIVRESTLLPFK 180
            +ENRFE SDS L+R VADKP GV  HATTEVGLR  R  LNSP Q EE  RESTLLPFK
Sbjct: 126 EQENRFEASDSALIRRVADKPTGVAVHATTEVGLRHNRFQLNSPEQTEETTRESTLLPFK 185

Query: 181 TQPSCY---ASSSSNESFEKVPNHRG--ESNSPNSGEESRLISKFTSAEPFEQEDGVVSL 240
           TQPS Y   ASSSS+  F++  NH G  +S+SPN  EESRLI KF S + FEQ +GV+SL
Sbjct: 186 TQPSSYAANASSSSHGGFDRDSNHGGYSDSDSPNLEEESRLILKFRSNDEFEQREGVISL 245

Query: 241 RRMTKSDESIRVSLCTKELLAALLPLVSSRSSNVQINAVASVVNLSLDKSNKLKIVRAGF 300
           R++TKS+ESIR SLCTKE LAALLPLV SRS NVQINAVASVVNLSL+K+NKLKIVRAGF
Sbjct: 246 RKLTKSNESIRASLCTKEFLAALLPLVLSRSPNVQINAVASVVNLSLEKANKLKIVRAGF 305

Query: 301 VPPLIDVLDSGFDEPQEHAAGALFSLSLDDENKMAIGILGALPVLMNTVRSDSERTRHDS 360
           VP LIDVLD G  E QEHAAGALFSLSLDDENKMAIGILGALPVLMNT+RSDSERTR+DS
Sbjct: 306 VPLLIDVLDGGNTESQEHAAGALFSLSLDDENKMAIGILGALPVLMNTLRSDSERTRNDS 365

Query: 361 ALCLYHLTLNPSNRVKLVKLGAVPILLSLAKLEDSASRILLILCNIAVSVDGRSAMLDAN 420
           ALCLYHLTLNPSNRVKLVKLGAVPILLSL ++E   SRI+LILCNIAVSVDGRSAMLDAN
Sbjct: 366 ALCLYHLTLNPSNRVKLVKLGAVPILLSLTRIEGCTSRIVLILCNIAVSVDGRSAMLDAN 425

Query: 421 AVGCLVGILKEKTMDSESTRENCVVALHALSQGGFRFRGLAKEAGAVEVLREIEERGSER 480
           AVGCLVG+LK+K MDSESTRENCVVAL+ALSQGGFRFRGLAKEAGAVEVLRE+EERG+ER
Sbjct: 426 AVGCLVGLLKDKAMDSESTRENCVVALYALSQGGFRFRGLAKEAGAVEVLREVEERGTER 485

Query: 481 AREKAKRILQMMR 486
           AREKAKRILQMMR
Sbjct: 486 AREKAKRILQMMR 498

BLAST of MS020048 vs. TAIR 10
Match: AT5G65200.1 (plant U-box 38 )

HSP 1 Score: 461.1 bits (1185), Expect = 1.1e-129
Identity = 269/508 (52.95%), Postives = 354/508 (69.69%), Query Frame = 0

Query: 1   NHRLKLSLFHHRSPSESKPG-----RPKEYPQECLCPISNSLMADPVVVSSGHTFDRLCV 60
           N RL+ + F HRS S +        + ++ P E LCPIS S+M+DPVVVSSG TF+R+CV
Sbjct: 4   NGRLRWNPFSHRSSSSTSSSSRQQQQEQQPPVEFLCPISKSVMSDPVVVSSGQTFERVCV 63

Query: 61  QVCQDLGFLPKLEDG--STPDFSSVITNRNVRFTILKWCDNNGIEHPREPDYRSIELIVR 120
           QVC+DL F+PKL D   S PDFS++I N N++ TI  WCD  G+  P+ PDY ++E I+R
Sbjct: 64  QVCRDLNFIPKLNDDEESLPDFSNIIPNLNMKSTIDTWCDTVGVSRPQPPDYSTVERILR 123

Query: 121 QLMEKERRENRFEVSDSELLRGVADKPQGVLNHATTEVGLRRLHLNSPPQGEE--IVRES 180
           Q M     E R  VS+ ELLR VA +   +++HA +E+  RR   NS    +E  IV  S
Sbjct: 124 QQMPPPDVEIR--VSEQELLRAVAHRAPMIIHHADSELMGRRDFNNSTTSSDESVIVAHS 183

Query: 181 --TLLPFKTQPSCY--ASSSSNESFEKVPNHRGESNSPNSG--EESRLISKFTSAEPFEQ 240
             T LP  T+P+C+  + SSS+   E + +H   SNS ++   E+  + +K  S+E F+Q
Sbjct: 184 PFTPLPLTTRPACFSPSPSSSSSEIETLTHHTFFSNSTSTATEEDEVIYNKLKSSEIFDQ 243

Query: 241 EDGVVSLRRMTKSDESIRVSLCTKELLAALLPLVSSRSSNVQINAVASVVNLSLDKSNKL 300
           E G++ +R+MT++++  RVSLC+  +L+ L  ++ SR S VQ NA+AS+VNLSLDK NKL
Sbjct: 244 EQGLIMMRKMTRTNDEARVSLCSPRILSLLKNMIVSRYSLVQTNALASLVNLSLDKKNKL 303

Query: 301 KIVRAGFVPPLIDVLDSGFDEPQEHAAGALFSLSLDDENKMAIGILGALPVLMNTVR-SD 360
            IVR GFVP LIDVL SG  E QEHAAG +FSLSL+D+NKM IG+LGAL  L++ +R ++
Sbjct: 304 TIVRLGFVPILIDVLKSGSREAQEHAAGTIFSLSLEDDNKMPIGVLGALQPLLHALRAAE 363

Query: 361 SERTRHDSALCLYHLTLNPSNRVKLVKLGAVPILLSLAKLEDSASRILLILCNIAVSVDG 420
           S+RTRHDSAL LYHLTLN +NR KLV+LGAVP L S+ +  +SASR LL++CN+A   +G
Sbjct: 364 SDRTRHDSALALYHLTLNQTNRSKLVRLGAVPALFSMVRSGESASRALLVICNLACCSEG 423

Query: 421 RSAMLDANAVGCLVGILKE-------KTMDSESTRENCVVALHALSQGGFRFRGLAKEAG 480
           RSAMLDANAV  LVG L+E       +   S S RENCV AL ALS    RF+GLAKEA 
Sbjct: 424 RSAMLDANAVAILVGKLREEWTEEPTEARSSSSARENCVAALFALSHESLRFKGLAKEAR 483

Query: 481 AVEVLREIEERGSERAREKAKRILQMMR 486
           AVEVL+E+EERG+ERAREKAK+ILQ+MR
Sbjct: 484 AVEVLKEVEERGTERAREKAKKILQLMR 509

BLAST of MS020048 vs. TAIR 10
Match: AT5G62560.1 (RING/U-box superfamily protein with ARM repeat domain )

HSP 1 Score: 402.9 bits (1034), Expect = 3.6e-112
Identity = 242/503 (48.11%), Postives = 317/503 (63.02%), Query Frame = 0

Query: 9   FHHRSPSESKPGRPK----EYPQECLCPISNSLMADPVVVSSGHTFDRLCVQVCQDLGFL 68
           FH RS S +    P+    E P E LCPI+  LM+DPVVVSSG TF+RL VQVC++LG++
Sbjct: 11  FHQRSSSATTTTLPQHKHDETPPEFLCPITGFLMSDPVVVSSGQTFERLSVQVCRNLGYI 70

Query: 69  PKLEDGSTPDFSSVITNRNVRFTILKWCDNNGIEHPREPDYRSIELIVRQLMEKERRENR 128
           P L DG+ PD S+VI N  ++ TI  WCD   ++HPR PD   +E +VR  M+K+   + 
Sbjct: 71  PDLLDGTRPDLSTVIPNLAMKSTIFSWCDRQKVDHPRPPDAAYVEGVVRARMDKDPNPSP 130

Query: 129 FEV-------SDSELLRGVADKPQGVLNHATTEVGLRRLHLNSPPQGEEIVR--ESTLLP 188
            +         + E+L  V +      +     +  R  +  SP    E V   +S+  P
Sbjct: 131 GQSPGPGDKDPEPEILPPVEENSPSDYDAVMEAIRARSKNSMSPTTSLESVTIGQSSYHP 190

Query: 189 FKTQPSCYASSSSNES---------FEKVPNHRG--ESNSPNSGEESRLISKFTSAEPFE 248
            +      +S++S+ S         F    +      S+SP S EE  + +K    + F+
Sbjct: 191 VRAVSMFSSSTTSSSSGVFAGADSPFRNAMSFSSTDHSSSPMSPEEEEIFNKLRGTDIFD 250

Query: 249 QEDGVVSLRRMTKSDESIRVSLCTKELLAALLPLVSSRSSNVQINAVASVVNLSLDKSNK 308
            E G++ LR+MT+S E +RVSLCT  +L+ L  L+ SR + VQ NA ASVVNLSL+K NK
Sbjct: 251 HEQGLILLRKMTRSSEDLRVSLCTDRILSFLRSLLVSRYNLVQTNAAASVVNLSLEKQNK 310

Query: 309 LKIVRAGFVPPLIDVLDSGFDEPQEHAAGALFSLSLDDENKMAIGILGALPVLMNTVR-S 368
           +KIVR+GFVP LIDVL SG  E QEH AGALFSL+L+DENKM IG+LGA+  L++ +R S
Sbjct: 311 VKIVRSGFVPLLIDVLKSGTTEAQEHVAGALFSLALEDENKMVIGVLGAVEPLLHALRSS 370

Query: 369 DSERTRHDSALCLYHLTLNPSNRVKLVKLGAVPILLSLAKLEDSASRILLILCNIAVSVD 428
           +SER R D+AL LYHL+L PSNR +LV+ GAVP LLS+ +  DS SRILL+LCN+A   D
Sbjct: 371 ESERARQDAALALYHLSLIPSNRTRLVRAGAVPTLLSMVRSGDSTSRILLVLCNLAACPD 430

Query: 429 GRSAMLDANAVGCLVGILKE-KTMDSESTRENCVVALHALSQGGFRFRGLAKEAGAVEVL 486
           G+ AMLD NAV  LVG L+E    DSE+ RENCV  L  L QG  RFRGLA EAGA EVL
Sbjct: 431 GKGAMLDGNAVAILVGKLREVGGGDSEAARENCVAVLLTLCQGNLRFRGLASEAGAEEVL 490

BLAST of MS020048 vs. TAIR 10
Match: AT5G40140.1 (RING/U-box superfamily protein with ARM repeat domain )

HSP 1 Score: 398.3 bits (1022), Expect = 8.9e-111
Identity = 229/485 (47.22%), Postives = 309/485 (63.71%), Query Frame = 0

Query: 3   RLKLSLFHHRSPSESKPGRPKEYPQECLCPISNSLMADPVVVSSGHTFDRLCVQVCQDLG 62
           R  LS     S S +      E P E LCPIS SLMADP++VSSGH+++R CV  C+ LG
Sbjct: 36  RTSLSRSSSSSSSNNNSPTKTEIPAEFLCPISGSLMADPIIVSSGHSYERACVIACKTLG 95

Query: 63  FLPKLEDGSTPDFSSVITNRNVRFTILKWCDNNGIEHPREPDYRSIELIVRQLMEKERRE 122
           F P       PDFS+VI N  ++  I  WC+      P+  +  + E ++  LMEK+ + 
Sbjct: 96  FTPT----PPPDFSTVIPNLALKSAIHSWCERRCFPPPKPLNSAAAEKLILALMEKKPQR 155

Query: 123 NRFEVSDSELLRGVADKPQGVLNHATTEVGLRRLHLNSPPQGEEIVRESTLLPFKTQPSC 182
            +  VS+ EL++ + DKP   LNHA TE+  R  + NS    E I   S  L   T+PSC
Sbjct: 156 RKVSVSEKELIQAIRDKPSVRLNHAATELDRRPNYFNS-SSDESIASSSRTLQLTTKPSC 215

Query: 183 YASSSSNESFEKVPNHRGESNSPN-SGEESRLISKFTSAEPFEQEDGVVSLRRMTKSDES 242
           ++S SS E          ES  PN + EE  L++K  S    E E+ ++S+RR+T+ DES
Sbjct: 216 FSSPSSGEI---------ESLEPNLTPEEEALLTKLKSNRISEIEEALISIRRITRIDES 275

Query: 243 IRVSLCTKELLAALLPLVSSRSSNVQINAVASVVNLSLDKSNKLKIVRAGFVPPLIDVLD 302
            R+SLCT  +++AL  L+ SR + VQ+N  A +VNLSL+KSNK+KIVR+G VPPLIDVL 
Sbjct: 276 SRISLCTTRVISALKSLIVSRYATVQVNVTAVLVNLSLEKSNKVKIVRSGIVPPLIDVLK 335

Query: 303 SGFDEPQEHAAGALFSLSLDDENKMAIGILGALPVLMNTVRSDSERTRHDSALCLYHLTL 362
            G  E QEH+AG +FSL+L+DENK AIG+LG L  L++ +R  +E TRHDSAL LYHL+L
Sbjct: 336 CGSVEAQEHSAGVIFSLALEDENKTAIGVLGGLEPLLHLIRVGTELTRHDSALALYHLSL 395

Query: 363 NPSNRVKLVKLGAVPILLSLAKLEDSASRILLILCNIAVSVDGRSAMLDANAVGCLVGIL 422
             SNR KLVKLGAV +LL +  L     R+LLILCN+A     R A+LD+  V C+VG+L
Sbjct: 396 VQSNRGKLVKLGAVQMLLGMVSLGQMIGRVLLILCNMASCPVSRPALLDSGGVECMVGVL 455

Query: 423 KEKTMDSESTRENCVVALHALS-QGGFRFRGLAKEAGAVEVLREIEERGSERAREKAKRI 482
           +     +ESTRE+CV  L+ LS  GG RF+GLA  A AVE L ++E  G ERA++KA+R+
Sbjct: 456 RRDREVNESTRESCVAVLYGLSHDGGLRFKGLAMAANAVEELVKVERSGRERAKQKARRV 506

Query: 483 LQMMR 486
           L+++R
Sbjct: 516 LEVLR 506

BLAST of MS020048 vs. TAIR 10
Match: AT3G47820.1 (PLANT U-BOX 39 )

HSP 1 Score: 395.2 bits (1014), Expect = 7.6e-110
Identity = 232/482 (48.13%), Postives = 311/482 (64.52%), Query Frame = 0

Query: 14  PSESKPGRPKEYPQECLCPISNSLMADPVVVSSGHTFDRLCVQVCQDLGFLPKLEDGSTP 73
           P  + PG   E P E LCPI+  LM+DPVVV+SG TF+R+ VQVC++L F PKL DG+ P
Sbjct: 2   PQHNSPG---ETPTEFLCPITGFLMSDPVVVASGQTFERISVQVCRNLSFAPKLHDGTQP 61

Query: 74  DFSSVITNRNVRFTILKWCDNNGIEHPREPDYRSIELIVRQLMEKERRENRFEVSDSELL 133
           D S+VI N  ++ TIL WCD N +EHPR PDY  +E +VR  M+     +   ++ SE+L
Sbjct: 62  DLSTVIPNLAMKSTILSWCDRNKMEHPRPPDYAYVEGVVRTRMDSLPPGSGHRIAKSEIL 121

Query: 134 RGVADKPQGVLNHATTEVGLRRLHLNSPPQGEEIVRESTLLPF-KTQPSCYA----SSSS 193
             VA+      ++ +    +R     S       +  +T LP  +T+P  ++    SS S
Sbjct: 122 PPVAENSNSNSDYDSVMGAIRSRSRTS-------LSSTTSLPLHQTRPINHSTRIQSSFS 181

Query: 194 NESFEKVPNHRGESNSPNSGEESRLISKFTSAEPFEQEDGVVSLRRMTKSDESIRVSLCT 253
              +   P        P S EE  + +K TS +  + E G++ LR+ T+S+E+ R+SLCT
Sbjct: 182 TSDYSSFP--------PMSPEEEEIYNKLTSVDTIDHEQGLIQLRKTTRSNETTRISLCT 241

Query: 254 KELLAALLPLVSSRSSNVQINAVASVVNLSLDKSNKLKIVRAGFVPPLIDVLDSGFDEPQ 313
             +L+ L  L+ SR + VQ NA AS+VNLSL+K NKLKIVR+GFVP LIDVL SG  E Q
Sbjct: 242 DRILSLLRSLIVSRYNIVQTNAAASIVNLSLEKPNKLKIVRSGFVPLLIDVLKSGSTEAQ 301

Query: 314 EHAAGALFSLSLDDENKMAIGILGALPVLMNTVR-SDSERTRHDSALCLYHLTLNPSNRV 373
           EH  GALFSL++++ENKM IG+LGA+  L++ +R S+SER R D+AL LYHL+L P+NR 
Sbjct: 302 EHVIGALFSLAVEEENKMVIGVLGAVEPLLHALRSSESERARQDAALALYHLSLIPNNRS 361

Query: 374 KLVKLGAVPILLSLAKLEDSASRILLILCNIAVSVDGRSAMLDANAVGCLVGILKEK--T 433
           +LVK GAVP++LS+ +  +SASRILL+LCN+A   +G+ AMLD NAV  LVG L+E    
Sbjct: 362 RLVKAGAVPMMLSMIRSGESASRILLLLCNLAACSEGKGAMLDGNAVSILVGKLRESGGA 421

Query: 434 MDSESTRENCVVALHALSQGGFRFRGLAKEAGAVEVLREI--EERGSERAREKAKRILQM 486
               + RENCV AL  LS G  RFRGLA EAGA E+L EI   E GS R +EKA +ILQ 
Sbjct: 422 ESDAAARENCVGALLTLSVGNMRFRGLASEAGAEEILTEIVESESGSGRLKEKASKILQT 465

BLAST of MS020048 vs. TAIR 10
Match: AT2G28830.1 (PLANT U-BOX 12 )

HSP 1 Score: 161.8 bits (408), Expect = 1.4e-39
Identity = 137/462 (29.65%), Postives = 215/462 (46.54%), Query Frame = 0

Query: 26  PQECLCPISNSLMADPVVVSSGHTFDRLCVQVCQDLGFL--PKLEDGSTPDFSSVITNRN 85
           P+E  CPIS  LM DPV+VSSG T++R C++   + G L  PK ++  T D   +  N  
Sbjct: 257 PEEFRCPISLELMTDPVIVSSGQTYERECIKKWLEGGHLTCPKTQETLTSDI--MTPNYV 316

Query: 86  VRFTILKWCDNNGIEHPREPDYRSIELIVRQLMEKERRENRFEVSDSELLRGVADKPQGV 145
           +R  I +WC++NGIE P+ P+                                       
Sbjct: 317 LRSLIAQWCESNGIEPPKRPNI-------------------------------------- 376

Query: 146 LNHATTEVGLRRLHLNSPPQGEEIVRESTLLPFKTQPSCYASSSSNESFEKVPNHRGESN 205
                                             +QPS  ASSSS+             +
Sbjct: 377 ----------------------------------SQPSSKASSSSS----------APDD 436

Query: 206 SPNSGEESRLISKFTSAEPFEQEDGVVSLRRMTKSDESIRVSLCTKELLAALLPLVS-SR 265
             N  EE  L+ K TS +P ++      +R + K +   RV++     +  L+ L++ S 
Sbjct: 437 EHNKIEE--LLLKLTSQQPEDRRSAAGEIRLLAKQNNHNRVAIAASGAIPLLVNLLTISN 496

Query: 266 SSNVQINAVASVVNLSLDKSNKLKIV-RAGFVPPLIDVLDSGFDEPQEHAAGALFSLSLD 325
            S  Q +AV S++NLS+ + NK KIV  +G VP ++ VL  G  E +E+AA  LFSLS+ 
Sbjct: 497 DSRTQEHAVTSILNLSICQENKGKIVYSSGAVPGIVHVLQKGSMEARENAAATLFSLSVI 556

Query: 326 DENKMAIGILGALPVLMNTVRSDSERTRHDSALCLYHLTLNPSNRVKLVKLGAVPILLSL 385
           DENK+ IG  GA+P L+  +   S+R + D+A  L++L +   N+ K V+ G VP+L+ L
Sbjct: 557 DENKVTIGAAGAIPPLVTLLSEGSQRGKKDAATALFNLCIFQGNKGKAVRAGLVPVLMRL 616

Query: 386 AKLEDS--ASRILLILCNIAVSVDGRSAMLDANAVGCLVGILKEKTMDSESTRENCVVAL 445
               +S      L IL  ++   DG+S +  A+AV  LV  ++     S   +EN    L
Sbjct: 617 LTEPESGMVDESLSILAILSSHPDGKSEVGAADAVPVLVDFIRS---GSPRNKENSAAVL 629

Query: 446 HALSQGGFRFRGLAKEAGAVEVLREIEERGSERAREKAKRIL 482
             L     +    A++ G +++L E+ E G++R + KA ++L
Sbjct: 677 VHLCSWNQQHLIEAQKLGIMDLLIEMAENGTDRGKRKAAQLL 629

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038895164.12.1e-21582.83U-box domain-containing protein 38-like [Benincasa hispida][more]
XP_022923679.13.8e-21280.61U-box domain-containing protein 38-like [Cucurbita moschata] >KAG7019833.1 U-box... [more]
XP_023519818.18.4e-21280.61U-box domain-containing protein 38-like [Cucurbita pepo subsp. pepo][more]
XP_023001501.12.7e-21079.71U-box domain-containing protein 38-like [Cucurbita maxima][more]
XP_004152547.13.5e-21080.32U-box domain-containing protein 38 [Cucumis sativus] >KGN64288.1 hypothetical pr... [more]
Match NameE-valueIdentityDescription
Q9FJP61.6e-12852.95U-box domain-containing protein 38 OS=Arabidopsis thaliana OX=3702 GN=PUB38 PE=1... [more]
Q0WUF65.1e-11148.11U-box domain-containing protein 41 OS=Arabidopsis thaliana OX=3702 GN=PUB41 PE=2... [more]
Q9STT15.7e-11047.97U-box domain-containing protein 39 OS=Arabidopsis thaliana OX=3702 GN=PUB39 PE=1... [more]
Q9FL171.3e-10947.22U-box domain-containing protein 40 OS=Arabidopsis thaliana OX=3702 GN=PUB40 PE=2... [more]
Q9ZV312.0e-3829.65U-box domain-containing protein 12 OS=Arabidopsis thaliana OX=3702 GN=PUB12 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1E7341.8e-21280.61RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111431320... [more]
A0A6J1KIT11.3e-21079.71RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111495624 P... [more]
A0A0A0LR641.7e-21080.32RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_1G045860 PE... [more]
A0A5A7ULP56.1e-20880.32RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A5D3BJ601.0e-20780.32RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
Match NameE-valueIdentityDescription
AT5G65200.11.1e-12952.95plant U-box 38 [more]
AT5G62560.13.6e-11248.11RING/U-box superfamily protein with ARM repeat domain [more]
AT5G40140.18.9e-11147.22RING/U-box superfamily protein with ARM repeat domain [more]
AT3G47820.17.6e-11048.13PLANT U-BOX 39 [more]
AT2G28830.11.4e-3929.65PLANT U-BOX 12 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 28..91
e-value: 4.5E-6
score: 36.2
IPR003613U box domainPFAMPF04564U-boxcoord: 26..59
e-value: 1.3E-6
score: 28.5
IPR003613U box domainPROSITEPS51698U_BOXcoord: 24..98
score: 20.39995
IPR000225ArmadilloSMARTSM00185arm_5coord: 321..361
e-value: 3.2
score: 16.7
coord: 280..320
e-value: 0.13
score: 21.4
coord: 401..444
e-value: 1.7
score: 17.7
coord: 362..400
e-value: 51.0
score: 7.8
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 291..328
score: 9.4324
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 250..292
score: 8.837399
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 24..99
e-value: 7.4E-12
score: 47.1
NoneNo IPR availablePFAMPF05804KAPcoord: 249..465
e-value: 4.2E-5
score: 21.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 179..213
NoneNo IPR availablePANTHERPTHR23315U BOX DOMAIN-CONTAININGcoord: 2..484
NoneNo IPR availablePANTHERPTHR23315:SF276U-BOX DOMAIN-CONTAINING PROTEIN 38coord: 2..484
NoneNo IPR availableCDDcd16453RING-Uboxcoord: 30..58
e-value: 7.03945E-11
score: 55.2012
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 15..100
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 206..483
e-value: 5.8E-43
score: 149.0
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 213..484

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS020048.1MS020048.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0005515 protein binding
molecular_function GO:0004842 ubiquitin-protein transferase activity