Homology
BLAST of MS020004 vs. NCBI nr
Match:
XP_022137045.1 (galactinol--sucrose galactosyltransferase-like [Momordica charantia])
HSP 1 Score: 1520.4 bits (3935), Expect = 0.0e+00
Identity = 718/726 (98.90%), Postives = 722/726 (99.45%), Query Frame = 0
Query: 1 PSPLISETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCG 60
PSPLISETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCG
Sbjct: 56 PSPLISETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCG 115
Query: 61 SDVQHETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRAS 120
SDVQHETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRAS
Sbjct: 116 SDVQHETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRAS 175
Query: 121 RFRSCLYVQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVN 180
RFRSCLY+QVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVN
Sbjct: 176 RFRSCLYIQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVN 235
Query: 181 PQGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLI 240
PQGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLI
Sbjct: 236 PQGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLI 295
Query: 241 KFEENYKFREYKSDRENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMPL 300
KFEENYKFREYKSDRENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMPL
Sbjct: 296 KFEENYKFREYKSDRENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMPL 355
Query: 301 SRIISPKLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVDV 360
SRIISP LSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVDV
Sbjct: 356 SRIISPNLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVDV 415
Query: 361 IHLLEMLSEEFGGRIELAKAYYKALTMSIHKHFKGNGVIASMEHCNDFMYLGTEAITLGR 420
IHLLEMLSEEFGGRIELAKAYY ALTMSIHK+FKGNGVIASMEHCNDFMYLGTEAITLGR
Sbjct: 416 IHLLEMLSEEFGGRIELAKAYYTALTMSIHKYFKGNGVIASMEHCNDFMYLGTEAITLGR 475
Query: 421 VGDDFWTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAIS 480
VGDDFWTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAIS
Sbjct: 476 VGDDFWTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAIS 535
Query: 481 GGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKIWNLN 540
GGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILK WNLN
Sbjct: 536 GGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKFWNLN 595
Query: 541 KFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFFA 600
KFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFFA
Sbjct: 596 KFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFFA 655
Query: 601 IYMVRQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVEFAPIGLADMLNPGGAI 660
IYMVRQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYV FAPIGLA+MLNPGGAI
Sbjct: 656 IYMVRQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVGFAPIGLANMLNPGGAI 715
Query: 661 QSLEIDENEGLVRVGARGCGEILVFASEGPRSCKIDGEEVGFEYEDDQMVKIQVPWPGSS 720
QSLEIDENEGLVRVGARGCGEILVFASEGPRSCKIDGE+VGFEYEDDQMVKIQVPWPGSS
Sbjct: 716 QSLEIDENEGLVRVGARGCGEILVFASEGPRSCKIDGEDVGFEYEDDQMVKIQVPWPGSS 775
Query: 721 RLSIIE 727
RLSIIE
Sbjct: 776 RLSIIE 781
BLAST of MS020004 vs. NCBI nr
Match:
XP_038894030.1 (galactinol--sucrose galactosyltransferase-like [Benincasa hispida])
HSP 1 Score: 1293.1 bits (3345), Expect = 0.0e+00
Identity = 605/731 (82.76%), Postives = 664/731 (90.83%), Query Frame = 0
Query: 1 PSPLISET---ETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVG 60
PS +IS +T ++GCFVGFDA+E S H+A IG L I+FSS+FRFKVWWTT W G
Sbjct: 46 PSNIISTPSPFKTNNSLGCFVGFDANEPRSSHLAPIGNLTDIKFSSLFRFKVWWTTHWAG 105
Query: 61 TCGSDVQHETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCV 120
TCG D+QHETQ+MILD + GRPFVLLLPILEGAFRCSLRPG D DD+VAMWVESGST V
Sbjct: 106 TCGRDIQHETQIMILDTNHHGRPFVLLLPILEGAFRCSLRPGDDHDDSVAMWVESGSTTV 165
Query: 121 RASRFRSCLYVQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYL 180
+ASRFRSCLY+QVGEDPY+LVK+AM+ VKL LGTFKLL++KTPP I+DKFGWCTWDAFYL
Sbjct: 166 QASRFRSCLYMQVGEDPYTLVKEAMEVVKLHLGTFKLLQDKTPPPIVDKFGWCTWDAFYL 225
Query: 181 KVNPQGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPC 240
KVNPQGIRTGVKCLVDGGCPPGM+LIDDGWQSIAHDADSITD QEAMDLT AGEQMPC
Sbjct: 226 KVNPQGIRTGVKCLVDGGCPPGMILIDDGWQSIAHDADSITD-HHQEAMDLTAAGEQMPC 285
Query: 241 RLIKFEENYKFREYKSDRENCK-GLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVL 300
RLIKFEENYKFR+Y+S + G+G FV+DLKEEFR++E+VYVWHALCGYWGGIRPNV
Sbjct: 286 RLIKFEENYKFRDYESPGKGFDVGMGGFVRDLKEEFRTIEHVYVWHALCGYWGGIRPNVP 345
Query: 301 GMPLSRIISPKLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGV 360
GMPLSR+I+PKLSQ LE TMEDLAVDKIV++GIGLVPPELAHKMY+GLHSHLQSAGIDGV
Sbjct: 346 GMPLSRVITPKLSQGLEKTMEDLAVDKIVNNGIGLVPPELAHKMYDGLHSHLQSAGIDGV 405
Query: 361 KVDVIHLLEMLSEEFGGRIELAKAYYKALTMSIHKHFKGNGVIASMEHCNDFMYLGTEAI 420
KVDVIHLLEMLSEEFGGRIELAKAYYKALT SI KH +GNGVIASMEHCNDFMYLGTEAI
Sbjct: 406 KVDVIHLLEMLSEEFGGRIELAKAYYKALTSSIKKHLQGNGVIASMEHCNDFMYLGTEAI 465
Query: 421 TLGRVGDDFWTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAAS 480
LGRVGDDFWT D SGDPYWLQGCHMVHCAYNSLWMGN+IHPDWDMFQS HPCAEFHAAS
Sbjct: 466 ALGRVGDDFWTVDPSGDPYWLQGCHMVHCAYNSLWMGNIIHPDWDMFQSMHPCAEFHAAS 525
Query: 481 RAISGGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKI 540
RAISGGPIY+SDSVG HNF LLK LVLPDGSILRCQ++ALPTRDCLFEDPLHDGKT+LKI
Sbjct: 526 RAISGGPIYVSDSVGKHNFKLLKRLVLPDGSILRCQHYALPTRDCLFEDPLHDGKTMLKI 585
Query: 541 WNLNKFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGV 600
WNLNKFTGALGLFNCQGGGWCPKTR+NRR SEYARTLTCVAGPKDIEWNNG+ PIS+KGV
Sbjct: 586 WNLNKFTGALGLFNCQGGGWCPKTRKNRRTSEYARTLTCVAGPKDIEWNNGENPISLKGV 645
Query: 601 NFFAIYMVRQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVEFAPIGLADMLNP 660
N FAIYMVR +KL+LLK SENLE++IAPLNY+LLVVSPVT+LSKP +EFAPIGL +MLN
Sbjct: 646 NLFAIYMVRDEKLKLLKTSENLEFTIAPLNYELLVVSPVTILSKPNMEFAPIGLVNMLNC 705
Query: 661 GGAIQSLEIDENEGLVRVGARGCGEILVFASEGPRSCKIDGEEVGFEY-EDDQMVKIQVP 720
GGAIQSLEIDENEGLV G +GCGE+ VFAS+ PR+CKIDG++V FEY +DD+MVKIQVP
Sbjct: 706 GGAIQSLEIDENEGLVTAGVKGCGEMRVFASKEPRNCKIDGKDVEFEYDDDDKMVKIQVP 765
Query: 721 WPGSSRLSIIE 727
WP SSRLSIIE
Sbjct: 766 WPSSSRLSIIE 775
BLAST of MS020004 vs. NCBI nr
Match:
XP_023519339.1 (galactinol--sucrose galactosyltransferase-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1284.6 bits (3323), Expect = 0.0e+00
Identity = 603/727 (82.94%), Postives = 659/727 (90.65%), Query Frame = 0
Query: 1 PSPLISETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCG 60
P PL+ GCF+GF+A E SCH+ IG LRGIRFSSIFRFKVWWTT WVG+CG
Sbjct: 65 PKPLLG--------GCFLGFNAAEPKSCHLVPIGNLRGIRFSSIFRFKVWWTTHWVGSCG 124
Query: 61 SDVQHETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRAS 120
D+QHETQMMILD +DQGRPFVL LPILEGAFRCSLRPG GDDTVAMWVESGST V+AS
Sbjct: 125 GDIQHETQMMILDTNDQGRPFVLFLPILEGAFRCSLRPG--GDDTVAMWVESGSTTVQAS 184
Query: 121 RFRSCLYVQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVN 180
+FRSCLY+QVG+DPY+LV +AM VKL LGTFKLL++KTPP I+DKFGWCTWDAFYLKVN
Sbjct: 185 QFRSCLYMQVGKDPYTLVNEAMNVVKLHLGTFKLLDDKTPPPIVDKFGWCTWDAFYLKVN 244
Query: 181 PQGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLI 240
P G+RTGVKCLVDGGCPPGM+LIDDGWQSIAHDADSI D QEAMDLT AGEQMPCRLI
Sbjct: 245 PHGVRTGVKCLVDGGCPPGMILIDDGWQSIAHDADSIAD-PHQEAMDLTVAGEQMPCRLI 304
Query: 241 KFEENYKFREYKS-DRENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMP 300
K+EENYKFR+Y S + + GLGAFV+DLKEEFR++E+VYVWHALCGYWGGIRPNV GMP
Sbjct: 305 KYEENYKFRDYGSHGKGSGVGLGAFVRDLKEEFRTIEHVYVWHALCGYWGGIRPNVPGMP 364
Query: 301 LSRIISPKLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVD 360
LSR+ISPK S+ LEMTMEDLAVDKIV++G+G VPPELAH+MY+GLHSHLQSAGIDGVKVD
Sbjct: 365 LSRVISPKPSKGLEMTMEDLAVDKIVNNGVGFVPPELAHEMYDGLHSHLQSAGIDGVKVD 424
Query: 361 VIHLLEMLSEEFGGRIELAKAYYKALTMSIHKHFKGNGVIASMEHCNDFMYLGTEAITLG 420
VIHLLEMLSEEFGGRIELAKAYYKALT SI+KH +GNGVIASME CNDFMYLGTEAI LG
Sbjct: 425 VIHLLEMLSEEFGGRIELAKAYYKALTASINKHLQGNGVIASMEQCNDFMYLGTEAIALG 484
Query: 421 RVGDDFWTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAI 480
RVGDDFWT D SGDPYWLQGCHMVHCAYNSLWMGN+IHPDWDMFQS HPCAEFHAASRAI
Sbjct: 485 RVGDDFWTVDASGDPYWLQGCHMVHCAYNSLWMGNIIHPDWDMFQSTHPCAEFHAASRAI 544
Query: 481 SGGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKIWNL 540
SGGPIY+SDSVG HNF LLK LVLPDGSILRCQ++ALP+RDCLFEDPL DGKT+LKIWNL
Sbjct: 545 SGGPIYVSDSVGKHNFKLLKRLVLPDGSILRCQHYALPSRDCLFEDPLQDGKTMLKIWNL 604
Query: 541 NKFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFF 600
N+FTGALGLFNCQGGGWCPKTRQNRR SEYARTLTCVAGPKDIEWNNGK PIS+KGV F
Sbjct: 605 NQFTGALGLFNCQGGGWCPKTRQNRRTSEYARTLTCVAGPKDIEWNNGKNPISLKGVYLF 664
Query: 601 AIYMVRQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVEFAPIGLADMLNPGGA 660
AIYM+++KKL LLK SENLE++IAPL+Y+LLVVSPVTVLSKPYVEFAPIGL +MLN GGA
Sbjct: 665 AIYMIQEKKLRLLKTSENLEFTIAPLSYELLVVSPVTVLSKPYVEFAPIGLVNMLNCGGA 724
Query: 661 IQSLEIDENEGLVRVGARGCGEILVFASEGPRSCKIDGEEVGFEYEDDQMVKIQVPWPGS 720
IQSLEI+ENEGLVRVGARGCGE+ VFAS+ PRSCKIDGE+V FEY DD+MVKIQVPWP S
Sbjct: 725 IQSLEIEENEGLVRVGARGCGEMRVFASKEPRSCKIDGEDVEFEY-DDKMVKIQVPWPIS 779
Query: 721 SRLSIIE 727
SRLSIIE
Sbjct: 785 SRLSIIE 779
BLAST of MS020004 vs. NCBI nr
Match:
KAG7019802.1 (Galactinol--sucrose galactosyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1275.8 bits (3300), Expect = 0.0e+00
Identity = 602/731 (82.35%), Postives = 659/731 (90.15%), Query Frame = 0
Query: 1 PSPLISETET----QLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWV 60
PS LI +T L GCF+GFDA E SCH+ IG LRGIRFSSIFRFKVWWTT WV
Sbjct: 53 PSQLIFCHKTTIPKSLLGGCFLGFDAAEPKSCHLVPIGNLRGIRFSSIFRFKVWWTTHWV 112
Query: 61 GTCGSDVQHETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTC 120
G+CG D+QHETQMMILD +DQGRPFVL LPILEGAFRCSLRPG GDD VAMWVESGST
Sbjct: 113 GSCGGDIQHETQMMILDTNDQGRPFVLFLPILEGAFRCSLRPG--GDDNVAMWVESGSTT 172
Query: 121 VRASRFRSCLYVQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFY 180
V+AS+FRSCLY+QVG+DPY+LV + M VKL LGTFKLL++KTPP I+DKFGWCTWDAFY
Sbjct: 173 VQASQFRSCLYMQVGKDPYTLVNEGMNVVKLHLGTFKLLDDKTPPPIVDKFGWCTWDAFY 232
Query: 181 LKVNPQGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMP 240
LKVNP G+ TGVKCLV+GGCPPGM+LIDDGWQSIAHDADSI D QEAMDLT AGEQMP
Sbjct: 233 LKVNPHGVWTGVKCLVEGGCPPGMILIDDGWQSIAHDADSIAD-PHQEAMDLTVAGEQMP 292
Query: 241 CRLIKFEENYKFREYKS-DRENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNV 300
CRLIK+EENYKFR+Y S + + GLGAFV+DLKEEFR++E+VYVWHALCGYWGGIRPNV
Sbjct: 293 CRLIKYEENYKFRDYGSRGKGSGVGLGAFVRDLKEEFRTIEHVYVWHALCGYWGGIRPNV 352
Query: 301 LGMPLSRIISPKLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDG 360
GMPLSR+ISPK S+ LEMTMEDLAVDKIV++G+G VPPELAH+MY+GLHSHLQSAGIDG
Sbjct: 353 PGMPLSRVISPKPSKGLEMTMEDLAVDKIVNNGVGFVPPELAHEMYDGLHSHLQSAGIDG 412
Query: 361 VKVDVIHLLEMLSEEFGGRIELAKAYYKALTMSIHKHFKGNGVIASMEHCNDFMYLGTEA 420
VKVDVIHLLEMLSEEFGGRIELAKAYYKALT SI+KH +GNGVIASME CNDFMYLGTEA
Sbjct: 413 VKVDVIHLLEMLSEEFGGRIELAKAYYKALTASINKHLQGNGVIASMEQCNDFMYLGTEA 472
Query: 421 ITLGRVGDDFWTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAA 480
I LGRVGDDFWT D SGDPYWLQGCHMVHCAYNSLWMGN+IHPDWDMFQS HPCAEFHAA
Sbjct: 473 IALGRVGDDFWTVDASGDPYWLQGCHMVHCAYNSLWMGNIIHPDWDMFQSTHPCAEFHAA 532
Query: 481 SRAISGGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILK 540
SRAISGGPIY+SDSVG HNF LLK LVLPDGSILRCQ++ALP+RDCLFEDPL DGKT+LK
Sbjct: 533 SRAISGGPIYVSDSVGKHNFKLLKRLVLPDGSILRCQHYALPSRDCLFEDPLQDGKTMLK 592
Query: 541 IWNLNKFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKG 600
IWNLN+FTGALGLFNCQGGGWCPKTRQNRR SEYARTLTCVAGPKDIEWNNGK PIS+KG
Sbjct: 593 IWNLNQFTGALGLFNCQGGGWCPKTRQNRRTSEYARTLTCVAGPKDIEWNNGKNPISLKG 652
Query: 601 VNFFAIYMVRQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVEFAPIGLADMLN 660
V+ FAIYM+++KKL LLK SENLE++IAPL+Y+LLVVSPVTVLSKPYVEFAPIGL +MLN
Sbjct: 653 VHLFAIYMIQEKKLRLLKTSENLEFTIAPLSYELLVVSPVTVLSKPYVEFAPIGLVNMLN 712
Query: 661 PGGAIQSLEIDENEGLVRVGARGCGEILVFASEGPRSCKIDGEEVGFEYEDDQMVKIQVP 720
GGAIQSLEI+ENEGLVRVGARG GE+ VFAS+ PRSCKIDGE+V FEY DD+MVKIQVP
Sbjct: 713 CGGAIQSLEIEENEGLVRVGARGYGEMRVFASKEPRSCKIDGEDVEFEY-DDKMVKIQVP 772
Query: 721 WPGSSRLSIIE 727
WP SSRLSIIE
Sbjct: 773 WPISSRLSIIE 779
BLAST of MS020004 vs. NCBI nr
Match:
XP_004152562.2 (LOW QUALITY PROTEIN: galactinol--sucrose galactosyltransferase [Cucumis sativus])
HSP 1 Score: 1275.4 bits (3299), Expect = 0.0e+00
Identity = 595/728 (81.73%), Postives = 652/728 (89.56%), Query Frame = 0
Query: 1 PSPLISETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCG 60
PSP +T L +GCF+GFDA E S H+ IG L+GIRFSS+FRFKVWWTT WVGTCG
Sbjct: 55 PSPF--KTNNSL-LGCFIGFDATEPKSSHLVPIGNLKGIRFSSLFRFKVWWTTHWVGTCG 114
Query: 61 SDVQHETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRAS 120
D+QHETQMMILD + GRP+VLLLPILEGAFRCSLR G + DD+VAMWVESGST V AS
Sbjct: 115 RDIQHETQMMILDTNHHGRPYVLLLPILEGAFRCSLRSGDENDDSVAMWVESGSTTVHAS 174
Query: 121 RFRSCLYVQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVN 180
FRSCLY+QVG+DPYSL+K+AMK VKL LGTFKLL EKTPP I+DKFGWCTWDAFYLKVN
Sbjct: 175 SFRSCLYMQVGDDPYSLLKEAMKVVKLHLGTFKLLHEKTPPPIVDKFGWCTWDAFYLKVN 234
Query: 181 PQGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLI 240
PQGI+ GVKCLVDGGCPPGM+LIDDGWQSIAHDADS TD QEAMDLT AGEQMPCRLI
Sbjct: 235 PQGIKIGVKCLVDGGCPPGMILIDDGWQSIAHDADSFTD-HHQEAMDLTAAGEQMPCRLI 294
Query: 241 KFEENYKFREYKSDRENC-KGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMP 300
KFEENYKFR+Y SD + GLGAFV+DLKEEFR++E VYVWHALCGYWGGIRPNV MP
Sbjct: 295 KFEENYKFRDYGSDGKGVGVGLGAFVRDLKEEFRTIENVYVWHALCGYWGGIRPNVPRMP 354
Query: 301 LSRIISPKLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVD 360
LSR++ PKLSQ LE TMEDLAVDKIV++GIGLVPPELAH+MY+GLHSHLQSAGIDGVKVD
Sbjct: 355 LSRVVIPKLSQGLEKTMEDLAVDKIVNNGIGLVPPELAHEMYDGLHSHLQSAGIDGVKVD 414
Query: 361 VIHLLEMLSEEFGGRIELAKAYYKALTMSIHKHFKGNGVIASMEHCNDFMYLGTEAITLG 420
VIHLLEM+SEEFGGRIELAKAYYKALT SI KH +GNG IASMEHCNDFMYLGTEAI LG
Sbjct: 415 VIHLLEMISEEFGGRIELAKAYYKALTASIKKHLQGNGAIASMEHCNDFMYLGTEAIALG 474
Query: 421 RVGDDFWTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAI 480
RVGDDFWT D SGDPYWLQGCHMVHCAYNSLWMGN+IHPDWDMFQS HPCAEFHAASRAI
Sbjct: 475 RVGDDFWTVDPSGDPYWLQGCHMVHCAYNSLWMGNIIHPDWDMFQSTHPCAEFHAASRAI 534
Query: 481 SGGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKIWNL 540
SGGPIY+SDSVG HNFNLLK LVLPDGSILRCQ++ALPTRDCLFEDPLHDGKT+LKIWNL
Sbjct: 535 SGGPIYVSDSVGKHNFNLLKRLVLPDGSILRCQHYALPTRDCLFEDPLHDGKTMLKIWNL 594
Query: 541 NKFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFF 600
NKFTG LGLFNCQGGGWCPKTR+NRR SEYARTLTCVAGPKDIEWNNGK PIS+KGVN F
Sbjct: 595 NKFTGTLGLFNCQGGGWCPKTRKNRRTSEYARTLTCVAGPKDIEWNNGKNPISLKGVNLF 654
Query: 601 AIYMVRQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVEFAPIGLADMLNPGGA 660
AIYM+R KKL+LLK SENLE++IAPL Y+LLVVSP TVLSKP +EFAPIGL +MLN GGA
Sbjct: 655 AIYMIRDKKLKLLKTSENLEFTIAPLEYELLVVSPATVLSKPNMEFAPIGLVNMLNCGGA 714
Query: 661 IQSLEIDENEGLVRVGARGCGEILVFASEGPRSCKIDGEEVGFEY-EDDQMVKIQVPWPG 720
I+SLEIDENEGLV+VG RGCGE+ VFAS P +CK++GE+V FEY +DD+MVK+Q+PWP
Sbjct: 715 IESLEIDENEGLVKVGVRGCGEMRVFASNEPINCKMEGEDVEFEYDDDDKMVKLQIPWPS 774
Query: 721 SSRLSIIE 727
SS+LSIIE
Sbjct: 775 SSKLSIIE 778
BLAST of MS020004 vs. ExPASy Swiss-Prot
Match:
Q8VWN6 (Galactinol--sucrose galactosyltransferase OS=Pisum sativum OX=3888 GN=RFS PE=1 SV=1)
HSP 1 Score: 1088.2 bits (2813), Expect = 0.0e+00
Identity = 495/717 (69.04%), Postives = 600/717 (83.68%), Query Frame = 0
Query: 15 GCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCGSDVQHETQMMILDR 74
GCFVGF+ E+ S HV +G+L+GI+F+SIFRFKVWWTT WVGT G ++QHETQ++ILD+
Sbjct: 84 GCFVGFNTTEAKSHHVVPLGKLKGIKFTSIFRFKVWWTTHWVGTNGHELQHETQILILDK 143
Query: 75 S-DQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRASRFRSCLYVQVGED 134
+ GRP+VLLLPILE +FR SL+PG +D V M VESGST V S F++CLY+ + D
Sbjct: 144 NISLGRPYVLLLPILENSFRTSLQPG--LNDYVDMSVESGSTHVTGSTFKACLYLHLSND 203
Query: 135 PYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVNPQGIRTGVKCLVD 194
PY LVK+A+K ++ LGTFK LEEKTPP I++KFGWCTWDAFYLKV+P+G+ GVK L D
Sbjct: 204 PYRLVKEAVKVIQTKLGTFKTLEEKTPPSIIEKFGWCTWDAFYLKVHPKGVWEGVKALTD 263
Query: 195 GGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLIKFEENYKFREYKS 254
GGCPPG V+IDDGWQSI+HD D E++ M+ T+AGEQMPCRLIK+EENYKFREY++
Sbjct: 264 GGCPPGFVIIDDGWQSISHDDDDPV--TERDGMNRTSAGEQMPCRLIKYEENYKFREYEN 323
Query: 255 -DRENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMPLSRIISPKLSQEL 314
D KGL FV+DLKEEFRSVE VYVWHALCGYWGG+RP V GMP ++++ PKLS +
Sbjct: 324 GDNGGKKGLVGFVRDLKEEFRSVESVYVWHALCGYWGGVRPKVCGMPEAKVVVPKLSPGV 383
Query: 315 EMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVDVIHLLEMLSEEFG 374
+MTMEDLAVDKIV +G+GLVPP LA +M++G+HSHL+SAGIDGVKVDVIHLLE+LSEE+G
Sbjct: 384 KMTMEDLAVDKIVENGVGLVPPNLAQEMFDGIHSHLESAGIDGVKVDVIHLLELLSEEYG 443
Query: 375 GRIELAKAYYKALTMSIHKHFKGNGVIASMEHCNDFMYLGTEAITLGRVGDDFWTKDLSG 434
GR+ELAKAYYKALT S++KHFKGNGVIASMEHCNDF LGTEAI+LGRVGDDFW D SG
Sbjct: 444 GRVELAKAYYKALTSSVNKHFKGNGVIASMEHCNDFFLLGTEAISLGRVGDDFWCCDPSG 503
Query: 435 DP---YWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAISGGPIYISDS 494
DP YWLQGCHMVHCAYNSLWMGN IHPDWDMFQS HPCAEFHAASRAISGGP+Y+SD
Sbjct: 504 DPNGTYWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAEFHAASRAISGGPVYVSDC 563
Query: 495 VGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKIWNLNKFTGALGLF 554
VG+HNF LLKS VLPDGSILRCQ++ALPTRDCLFEDPLH+GKT+LKIWNLNK+ G LGLF
Sbjct: 564 VGNHNFKLLKSFVLPDGSILRCQHYALPTRDCLFEDPLHNGKTMLKIWNLNKYAGVLGLF 623
Query: 555 NCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFFAIYMVRQKKL 614
NCQGGGWCP+TR+N+ ASE++ +TC A P+DIEW NGKTP+ +KGV+ FA+Y ++KKL
Sbjct: 624 NCQGGGWCPETRRNKSASEFSHAVTCYASPEDIEWCNGKTPMDIKGVDVFAVYFFKEKKL 683
Query: 615 ELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVEFAPIGLADMLNPGGAIQSLEIDENE 674
L+K S+ LE S+ P +++L+ VSP+ V SK ++FAPIGL +MLN GGA+QSLE D++
Sbjct: 684 SLMKCSDRLEVSLEPFSFELMTVSPLKVFSKRLIQFAPIGLVNMLNSGGAVQSLEFDDSA 743
Query: 675 GLVRVGARGCGEILVFASEGPRSCKIDGEEVGFEYEDDQMVKIQVPWPGSSRLSIIE 727
LV++G RGCGE+ VFASE P CKIDG V F+YE D+MV++Q+ WPGSS LS++E
Sbjct: 744 SLVKIGVRGCGELSVFASEKPVCCKIDGVSVEFDYE-DKMVRVQILWPGSSTLSLVE 795
BLAST of MS020004 vs. ExPASy Swiss-Prot
Match:
Q9FND9 (Probable galactinol--sucrose galactosyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=RFS5 PE=1 SV=1)
HSP 1 Score: 999.2 bits (2582), Expect = 2.4e-290
Identity = 469/728 (64.42%), Postives = 571/728 (78.43%), Query Frame = 0
Query: 12 LAVGCFVGFDAD-ESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCGSDVQHETQMM 71
++ G F+GF+ D E S HVASIG+L+ IRF SIFRFKVWWTT WVG+ G D+++ETQ++
Sbjct: 62 VSAGSFIGFNLDGEPKSHHVASIGKLKNIRFMSIFRFKVWWTTHWVGSNGRDIENETQII 121
Query: 72 ILDR--------SDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRASRF 131
ILD+ S GRP+VLLLP+LEG+FR S + G DD VA+ VESGST V S F
Sbjct: 122 ILDQSGSDSGPGSGSGRPYVLLLPLLEGSFRSSFQSG--EDDDVAVCVESGSTEVTGSEF 181
Query: 132 RSCLYVQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVNPQ 191
R +YV G+DP+ LVKDAMK +++ + TFKLLEEK+PPGI+DKFGWCTWDAFYL VNP
Sbjct: 182 RQIVYVHAGDDPFKLVKDAMKVIRVHMNTFKLLEEKSPPGIVDKFGWCTWDAFYLTVNPD 241
Query: 192 GIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLIKF 251
G+ GVKCLVDGGCPPG+VLIDDGWQSI HD+D I + E M++T AGEQMPCRL+KF
Sbjct: 242 GVHKGVKCLVDGGCPPGLVLIDDGWQSIGHDSDGI----DVEGMNITVAGEQMPCRLLKF 301
Query: 252 EENYKFREYKSDR-ENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMPLS 311
EEN+KF++Y S + +N G+ AFV+DLK+EF +V+Y+YVWHALCGYWGG+RP +P S
Sbjct: 302 EENHKFKDYVSPKDQNDVGMKAFVRDLKDEFSTVDYIYVWHALCGYWGGLRPEAPALPPS 361
Query: 312 RIISPKLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVDVI 371
II P+LS L++TMEDLAVDKI+ +GIG P+LA + YEGLHSHLQ+AGIDGVKVDVI
Sbjct: 362 TIIRPELSPGLKLTMEDLAVDKIIETGIGFASPDLAKEFYEGLHSHLQNAGIDGVKVDVI 421
Query: 372 HLLEMLSEEFGGRIELAKAYYKALTMSIHKHFKGNGVIASMEHCNDFMYLGTEAITLGRV 431
H+LEML +++GGR++LAKAY+KALT S++KHF GNGVIASMEHCNDFM+LGTEAI+LGRV
Sbjct: 422 HILEMLCQKYGGRVDLAKAYFKALTSSVNKHFNGNGVIASMEHCNDFMFLGTEAISLGRV 481
Query: 432 GDDFWTKDLSGDP---YWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRA 491
GDDFW D SGDP +WLQGCHMVHCAYNSLWMGN I PDWDMFQS HPCAEFHAASRA
Sbjct: 482 GDDFWCTDPSGDPNGTFWLQGCHMVHCAYNSLWMGNFIQPDWDMFQSTHPCAEFHAASRA 541
Query: 492 ISGGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKIWN 551
ISGGPIYISD VG H+F+LLK LVLP+GSILRC+Y+ALPTRD LFEDPLHDGKT+LKIWN
Sbjct: 542 ISGGPIYISDCVGKHDFDLLKRLVLPNGSILRCEYYALPTRDRLFEDPLHDGKTMLKIWN 601
Query: 552 LNKFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNF 611
LNK+TG +G FNCQGGGWC +TR+N+ SE TLT PKD+EWN+G +PIS+ V
Sbjct: 602 LNKYTGVIGAFNCQGGGWCRETRRNQCFSECVNTLTATTSPKDVEWNSGSSPISIANVEE 661
Query: 612 FAIYMVRQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVEFAPIGLADMLNPGG 671
FA+++ + KKL L +++LE ++ P ++L+ VSPV + V FAPIGL +MLN G
Sbjct: 662 FALFLSQSKKLLLSGLNDDLELTLEPFKFELITVSPVVTIEGNSVRFAPIGLVNMLNTSG 721
Query: 672 AIQSLEIDENEGLVRVGARGCGEILVFASEGPRSCKIDGEEVGFEYEDDQMVKIQVPWPG 727
AI+SL N+ V VG G GE V+AS+ P SC IDGE V F YE D MV +QVPW G
Sbjct: 722 AIRSLVY--NDESVEVGVFGAGEFRVYASKKPVSCLIDGEVVEFGYE-DSMVMVQVPWSG 780
BLAST of MS020004 vs. ExPASy Swiss-Prot
Match:
Q5VQG4 (Galactinol--sucrose galactosyltransferase OS=Oryza sativa subsp. japonica OX=39947 GN=RFS PE=1 SV=1)
HSP 1 Score: 949.5 bits (2453), Expect = 2.2e-275
Identity = 445/728 (61.13%), Postives = 555/728 (76.24%), Query Frame = 0
Query: 13 AVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCGSDVQHETQMMIL 72
A G F+GFDA + HV IG+LR RF SIFRFKVWWTT WVGT G DV++ETQMMIL
Sbjct: 68 AAGSFLGFDAPAAKDRHVVPIGKLRDTRFMSIFRFKVWWTTHWVGTNGRDVENETQMMIL 127
Query: 73 DRSDQ------GRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRASRFRSCL 132
D+S RP+VLLLPI+EG FR L G +D V M +ESGS+ VR S FRS +
Sbjct: 128 DQSGTKSSPTGPRPYVLLLPIVEGPFRACLE-SGKAEDYVHMVLESGSSTVRGSVFRSAV 187
Query: 133 YVQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVNPQGIRT 192
Y+ G+DP+ LVKDAM+ V+ LGTF+L+EEKTPP I+DKFGWCTWDAFYLKV+P+G+
Sbjct: 188 YLHAGDDPFDLVKDAMRVVRAHLGTFRLMEEKTPPPIVDKFGWCTWDAFYLKVHPEGVWE 247
Query: 193 GVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLIKFEENY 252
GV+ L DGGCPPG+VLIDDGWQSI HD D + G E M+ T+AGEQMPCRLIKF+ENY
Sbjct: 248 GVRRLADGGCPPGLVLIDDGWQSICHDDDDLGSG--AEGMNRTSAGEQMPCRLIKFQENY 307
Query: 253 KFREYKSDRENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMPLSRIISP 312
KFREYK G+G FV+++K F +VE VYVWHALCGYWGG+RP G+P +++++P
Sbjct: 308 KFREYKG------GMGGFVREMKAAFPTVEQVYVWHALCGYWGGLRPGAPGLPPAKVVAP 367
Query: 313 KLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVDVIHLLEM 372
+LS L+ TMEDLAVDKIV++G+GLV P A ++YEGLHSHLQ++GIDGVKVDVIHLLEM
Sbjct: 368 RLSPGLQRTMEDLAVDKIVNNGVGLVDPRRARELYEGLHSHLQASGIDGVKVDVIHLLEM 427
Query: 373 LSEEFGGRIELAKAYYKALTMSIHKHFKGNGVIASMEHCNDFMYLGTEAITLGRVGDDFW 432
+ EE+GGR+ELAKAY+ LT S+ +HF GNGVIASMEHCNDFM LGTEA+ LGRVGDDFW
Sbjct: 428 VCEEYGGRVELAKAYFAGLTESVRRHFNGNGVIASMEHCNDFMLLGTEAVALGRVGDDFW 487
Query: 433 TKDLSGDP---YWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAISGGP 492
D SGDP +WLQGCHMVHCAYNSLWMG IHPDWDMFQS HPCA FHAASRA+SGGP
Sbjct: 488 CTDPSGDPDGTFWLQGCHMVHCAYNSLWMGAFIHPDWDMFQSTHPCAAFHAASRAVSGGP 547
Query: 493 IYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKIWNLNKFT 552
+Y+SD+VG H+F+LL+ L LPDG+ILRC+ +ALPTRDCLF DPLHDGKT+LKIWN+NKF+
Sbjct: 548 VYVSDAVGCHDFDLLRRLALPDGTILRCERYALPTRDCLFADPLHDGKTMLKIWNVNKFS 607
Query: 553 GALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFFAIYM 612
G LG FNCQGGGW + R+N A+ ++ +T A P D+EW++G G + FA+Y
Sbjct: 608 GVLGAFNCQGGGWSREARRNMCAAGFSVPVTARASPADVEWSHGG-----GGGDRFAVYF 667
Query: 613 VRQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPY--VEFAPIGLADMLNPGGAIQ 672
V +KL+LL+ E++E ++ P Y+LLVV+PV + P + FAPIGLA+MLN GGA+Q
Sbjct: 668 VEARKLQLLRRDESVELTLEPFTYELLVVAPVRAIVSPELGIGFAPIGLANMLNAGGAVQ 727
Query: 673 SLEIDENEGLV--RVGARGCGEILVFASEGPRSCKIDGEEVGFEYEDDQMVKIQVPWPGS 727
E +G V V +G GE++ ++S PR CK++G++ F+YED +V + VPW GS
Sbjct: 728 GFEAARKDGDVAAEVAVKGAGEMVAYSSARPRLCKVNGQDAEFKYEDG-IVTVDVPWTGS 780
BLAST of MS020004 vs. ExPASy Swiss-Prot
Match:
Q93XK2 (Stachyose synthase OS=Pisum sativum OX=3888 GN=STS1 PE=1 SV=1)
HSP 1 Score: 686.8 bits (1771), Expect = 2.7e-196
Identity = 347/793 (43.76%), Postives = 483/793 (60.91%), Query Frame = 0
Query: 1 PSPLISETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCG 60
P L+ + G F GF + + + SIG G F SIFRFK WW+TQW+G G
Sbjct: 58 PPSLLQKVLAYSHKGGFFGFSHETPSDRLMNSIGSFNGKDFLSIFRFKTWWSTQWIGKSG 117
Query: 61 SDVQHETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRAS 120
SD+Q ETQ ++++ + + +V+++PI+E FR +L PG +D V + ESGST V+ S
Sbjct: 118 SDLQMETQWILIE-VPETKSYVVIIPIIEKCFRSALFPG--FNDHVKIIAESGSTKVKES 177
Query: 121 RFRSCLYVQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVN 180
F S YV E+PY L+K+A A+++ L +F+LLEEKT P ++DKFGWCTWDAFYL VN
Sbjct: 178 TFNSIAYVHFSENPYDLMKEAYSAIRVHLNSFRLLEEKTIPNLVDKFGWCTWDAFYLTVN 237
Query: 181 PQGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLI 240
P GI G+ GG P V+IDDGWQSI+ D ++A +L GEQM RL
Sbjct: 238 PIGIFHGLDDFSKGGVEPRFVIIDDGWQSISFDGYD----PNEDAKNLVLGGEQMSGRLH 297
Query: 241 KFEENYKFREY------------------------------------------------- 300
+F+E YKFR+Y
Sbjct: 298 RFDECYKFRKYESGLLLGPNSPPYDPNNFTDLILKGIEHEKLRKKREEAISSKSSDLAEI 357
Query: 301 ------------------------KSDRENCKGLGAFVKDLKEEFRSVEYVYVWHALCGY 360
KS+ ++ GL AF KDL+ +F+ ++ VYVWHALCG
Sbjct: 358 ESKIKKVVKEIDDLFGGEQFSSGEKSEMKSEYGLKAFTKDLRTKFKGLDDVYVWHALCGA 417
Query: 361 WGGIRPNVLGMPLSRIISPKLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSH 420
WGG+RP + ++I+ KLS L+ TMEDLAV +I + +GLV P A+++Y+ +HS+
Sbjct: 418 WGGVRPETTHLD-TKIVPCKLSPGLDGTMEDLAVVEISKASLGLVHPSQANELYDSMHSY 477
Query: 421 LQSAGIDGVKVDVIHLLEMLSEEFGGRIELAKAYYKALTMSIHKHFKGNGVIASMEHCND 480
L +GI GVKVDVIH LE + +E+GGR++LAK YY+ LT SI K+F GNG+IASM+HCND
Sbjct: 478 LAESGITGVKVDVIHSLEYVCDEYGGRVDLAKVYYEGLTKSIVKNFNGNGMIASMQHCND 537
Query: 481 FMYLGTEAITLGRVGDDFWTKDLSGDP---YWLQGCHMVHCAYNSLWMGNMIHPDWDMFQ 540
F +LGT+ I++GRVGDDFW +D +GDP +WLQG HM+HC+YNSLWMG MI PDWDMFQ
Sbjct: 538 FFFLGTKQISMGRVGDDFWFQDPNGDPMGSFWLQGVHMIHCSYNSLWMGQMIQPDWDMFQ 597
Query: 541 SNHPCAEFHAASRAISGGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFE 600
S+H CA+FHA SRAI GGPIY+SD+VGSH+F+L+K LV PDG+I +C Y LPTRDCLF+
Sbjct: 598 SDHVCAKFHAGSRAICGGPIYVSDNVGSHDFDLIKKLVFPDGTIPKCIYFPLPTRDCLFK 657
Query: 601 DPLHDGKTILKIWNLNKFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEW 660
+PL D T+LKIWN NK+ G +G FNCQG GW P ++ R E + + ++EW
Sbjct: 658 NPLFDHTTVLKIWNFNKYGGVIGAFNCQGAGWDPIMQKFRGFPECYKPIPGTVHVTEVEW 717
Query: 661 NNGKTPISVKGVNFFAIYMVRQKKLELLK-ASENLEYSIAPLNYQLLVVSPVTVLSKPYV 717
+ + + + +Y+ + ++L L+ SE ++++I P ++L PVT L +
Sbjct: 718 DQKEETSHLGKAEEYVVYLNQAEELSLMTLKSEPIQFTIQPSTFELYSFVPVTKLCGG-I 777
BLAST of MS020004 vs. ExPASy Swiss-Prot
Match:
Q9SYJ4 (Probable galactinol--sucrose galactosyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=RFS4 PE=2 SV=3)
HSP 1 Score: 662.5 bits (1708), Expect = 5.4e-189
Identity = 343/801 (42.82%), Postives = 474/801 (59.18%), Query Frame = 0
Query: 1 PSPLISETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCG 60
P P++ + G F+GF + + S+GR F S+FRFK+WW+T W+G G
Sbjct: 72 PLPILLRVQANAHKGGFLGFTKESPSDRLTNSLGRFEDREFLSLFRFKMWWSTAWIGKSG 131
Query: 61 SDVQHETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRAS 120
SD+Q ETQ ++L + + +V ++P +EGAFR SL PG G+ V + ESGST V+ S
Sbjct: 132 SDLQAETQWVML-KIPEIDSYVAIIPTIEGAFRASLTPGEKGN--VLICAESGSTKVKES 191
Query: 121 RFRSCLYVQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVN 180
F+S Y+ + ++PY+L+K+A A+++ + TFKLLEEK P I+DKFGWCTWDA YL V+
Sbjct: 192 SFKSIAYIHICDNPYNLMKEAFSALRVHMNTFKLLEEKKLPKIVDKFGWCTWDACYLTVD 251
Query: 181 PQGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLI 240
P I TGVK DGG P V+IDDGWQSI D D + +++A +L GEQM RL
Sbjct: 252 PATIWTGVKEFEDGGVCPKFVIIDDGWQSINFDGDEL----DKDAENLVLGGEQMTARLT 311
Query: 241 KFEENYKFREYK------------------------------------------------ 300
F+E KFR YK
Sbjct: 312 SFKECKKFRNYKGGSFITSDASHFNPLKPKMLIYKATERIQAIILRRKLVKESGEQDLTE 371
Query: 301 --------------------------SDRENCKGLGAFVKDLKEEFRSVEYVYVWHALCG 360
SD + G+ AF KDL+ F+S++ +YVWHALCG
Sbjct: 372 LDEKIKILSEELNAMFDEVEKEESLGSDDVSGSGMAAFTKDLRLRFKSLDDIYVWHALCG 431
Query: 361 YWGGIRPNVLGMPLSRIISP-KLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLH 420
W G+RP + M L ++P +LS L TM DLAVDK+V +GIGLV P AH+ Y+ +H
Sbjct: 432 AWNGVRPETM-MDLKAKVAPFELSPSLGATMADLAVDKVVEAGIGLVHPSKAHEFYDSMH 491
Query: 421 SHLQSAGIDGVKVDVIHLLEMLSEEFGGRIELAKAYYKALTMSIHKHFKGNGVIASMEHC 480
S+L S G+ G K+DV LE L+EE GGR+ELAKAYY LT S+ K+F G VIASM+ C
Sbjct: 492 SYLASVGVTGAKIDVFQTLESLAEEHGGRVELAKAYYDGLTESMIKNFNGTDVIASMQQC 551
Query: 481 NDFMYLGTEAITLGRVGDDFWTKDLSGDP---YWLQGCHMVHCAYNSLWMGNMIHPDWDM 540
N+F +L T+ I++GRVGDDFW +D GDP YWLQG HM+HC+YNS+WMG MI PDWDM
Sbjct: 552 NEFFFLATKQISIGRVGDDFWWQDPYGDPQGVYWLQGVHMIHCSYNSIWMGQMIQPDWDM 611
Query: 541 FQSNHPCAEFHAASRAISGGPIYISDSVG--SHNFNLLKSLVLPDGSILRCQYHALPTRD 600
FQS+H CAE+HAASRAI GGP+Y+SD +G SHNF+L+K L DG+I RC ++ALPTRD
Sbjct: 612 FQSDHVCAEYHAASRAICGGPVYLSDHLGKASHNFDLIKKLAFFDGTIPRCVHYALPTRD 671
Query: 601 CLFEDPLHDGKTILKIWNLNKFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPK 660
LF++PL D ++ILKI+N NKF G +G FNCQG GW P+ + + E T++
Sbjct: 672 SLFKNPLFDKESILKIFNFNKFGGVIGTFNCQGAGWSPEEHRFKGYKECYTTVSGTVHVS 731
Query: 661 DIEWNNGKTPISVKGVNFFAIYMV-RQKKLELL---KASENLEYSIAPLNYQLLVVSPVT 717
DIEW+ + V + Y+V +Q+ E+L SE ++ ++ P + LL PVT
Sbjct: 732 DIEWDQNPEAAGSQ-VTYTGDYLVYKQQSEEILFMNSKSEAMKITLEPSAFDLLSFVPVT 791
BLAST of MS020004 vs. ExPASy TrEMBL
Match:
A0A6J1C5K5 (galactinol--sucrose galactosyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111008605 PE=3 SV=1)
HSP 1 Score: 1520.4 bits (3935), Expect = 0.0e+00
Identity = 718/726 (98.90%), Postives = 722/726 (99.45%), Query Frame = 0
Query: 1 PSPLISETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCG 60
PSPLISETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCG
Sbjct: 56 PSPLISETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCG 115
Query: 61 SDVQHETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRAS 120
SDVQHETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRAS
Sbjct: 116 SDVQHETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRAS 175
Query: 121 RFRSCLYVQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVN 180
RFRSCLY+QVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVN
Sbjct: 176 RFRSCLYIQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVN 235
Query: 181 PQGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLI 240
PQGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLI
Sbjct: 236 PQGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLI 295
Query: 241 KFEENYKFREYKSDRENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMPL 300
KFEENYKFREYKSDRENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMPL
Sbjct: 296 KFEENYKFREYKSDRENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMPL 355
Query: 301 SRIISPKLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVDV 360
SRIISP LSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVDV
Sbjct: 356 SRIISPNLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVDV 415
Query: 361 IHLLEMLSEEFGGRIELAKAYYKALTMSIHKHFKGNGVIASMEHCNDFMYLGTEAITLGR 420
IHLLEMLSEEFGGRIELAKAYY ALTMSIHK+FKGNGVIASMEHCNDFMYLGTEAITLGR
Sbjct: 416 IHLLEMLSEEFGGRIELAKAYYTALTMSIHKYFKGNGVIASMEHCNDFMYLGTEAITLGR 475
Query: 421 VGDDFWTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAIS 480
VGDDFWTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAIS
Sbjct: 476 VGDDFWTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAIS 535
Query: 481 GGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKIWNLN 540
GGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILK WNLN
Sbjct: 536 GGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKFWNLN 595
Query: 541 KFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFFA 600
KFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFFA
Sbjct: 596 KFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFFA 655
Query: 601 IYMVRQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVEFAPIGLADMLNPGGAI 660
IYMVRQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYV FAPIGLA+MLNPGGAI
Sbjct: 656 IYMVRQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVGFAPIGLANMLNPGGAI 715
Query: 661 QSLEIDENEGLVRVGARGCGEILVFASEGPRSCKIDGEEVGFEYEDDQMVKIQVPWPGSS 720
QSLEIDENEGLVRVGARGCGEILVFASEGPRSCKIDGE+VGFEYEDDQMVKIQVPWPGSS
Sbjct: 716 QSLEIDENEGLVRVGARGCGEILVFASEGPRSCKIDGEDVGFEYEDDQMVKIQVPWPGSS 775
Query: 721 RLSIIE 727
RLSIIE
Sbjct: 776 RLSIIE 781
BLAST of MS020004 vs. ExPASy TrEMBL
Match:
A0A0A0LWD8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G046280 PE=3 SV=1)
HSP 1 Score: 1275.4 bits (3299), Expect = 0.0e+00
Identity = 595/728 (81.73%), Postives = 652/728 (89.56%), Query Frame = 0
Query: 1 PSPLISETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCG 60
PSP +T L +GCF+GFDA E S H+ IG L+GIRFSS+FRFKVWWTT WVGTCG
Sbjct: 55 PSPF--KTNNSL-LGCFIGFDATEPKSSHLVPIGNLKGIRFSSLFRFKVWWTTHWVGTCG 114
Query: 61 SDVQHETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRAS 120
D+QHETQMMILD + GRP+VLLLPILEGAFRCSLR G + DD+VAMWVESGST V AS
Sbjct: 115 RDIQHETQMMILDTNHHGRPYVLLLPILEGAFRCSLRSGDENDDSVAMWVESGSTTVHAS 174
Query: 121 RFRSCLYVQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVN 180
FRSCLY+QVG+DPYSL+K+AMK VKL LGTFKLL EKTPP I+DKFGWCTWDAFYLKVN
Sbjct: 175 SFRSCLYMQVGDDPYSLLKEAMKVVKLHLGTFKLLHEKTPPPIVDKFGWCTWDAFYLKVN 234
Query: 181 PQGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLI 240
PQGI+ GVKCLVDGGCPPGM+LIDDGWQSIAHDADS TD QEAMDLT AGEQMPCRLI
Sbjct: 235 PQGIKIGVKCLVDGGCPPGMILIDDGWQSIAHDADSFTD-HHQEAMDLTAAGEQMPCRLI 294
Query: 241 KFEENYKFREYKSDRENC-KGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMP 300
KFEENYKFR+Y SD + GLGAFV+DLKEEFR++E VYVWHALCGYWGGIRPNV MP
Sbjct: 295 KFEENYKFRDYGSDGKGVGVGLGAFVRDLKEEFRTIENVYVWHALCGYWGGIRPNVPRMP 354
Query: 301 LSRIISPKLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVD 360
LSR++ PKLSQ LE TMEDLAVDKIV++GIGLVPPELAH+MY+GLHSHLQSAGIDGVKVD
Sbjct: 355 LSRVVIPKLSQGLEKTMEDLAVDKIVNNGIGLVPPELAHEMYDGLHSHLQSAGIDGVKVD 414
Query: 361 VIHLLEMLSEEFGGRIELAKAYYKALTMSIHKHFKGNGVIASMEHCNDFMYLGTEAITLG 420
VIHLLEM+SEEFGGRIELAKAYYKALT SI KH +GNG IASMEHCNDFMYLGTEAI LG
Sbjct: 415 VIHLLEMISEEFGGRIELAKAYYKALTASIKKHLQGNGAIASMEHCNDFMYLGTEAIALG 474
Query: 421 RVGDDFWTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAI 480
RVGDDFWT D SGDPYWLQGCHMVHCAYNSLWMGN+IHPDWDMFQS HPCAEFHAASRAI
Sbjct: 475 RVGDDFWTVDPSGDPYWLQGCHMVHCAYNSLWMGNIIHPDWDMFQSTHPCAEFHAASRAI 534
Query: 481 SGGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKIWNL 540
SGGPIY+SDSVG HNFNLLK LVLPDGSILRCQ++ALPTRDCLFEDPLHDGKT+LKIWNL
Sbjct: 535 SGGPIYVSDSVGKHNFNLLKRLVLPDGSILRCQHYALPTRDCLFEDPLHDGKTMLKIWNL 594
Query: 541 NKFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFF 600
NKFTG LGLFNCQGGGWCPKTR+NRR SEYARTLTCVAGPKDIEWNNGK PIS+KGVN F
Sbjct: 595 NKFTGTLGLFNCQGGGWCPKTRKNRRTSEYARTLTCVAGPKDIEWNNGKNPISLKGVNLF 654
Query: 601 AIYMVRQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVEFAPIGLADMLNPGGA 660
AIYM+R KKL+LLK SENLE++IAPL Y+LLVVSP TVLSKP +EFAPIGL +MLN GGA
Sbjct: 655 AIYMIRDKKLKLLKTSENLEFTIAPLEYELLVVSPATVLSKPNMEFAPIGLVNMLNCGGA 714
Query: 661 IQSLEIDENEGLVRVGARGCGEILVFASEGPRSCKIDGEEVGFEY-EDDQMVKIQVPWPG 720
I+SLEIDENEGLV+VG RGCGE+ VFAS P +CK++GE+V FEY +DD+MVK+Q+PWP
Sbjct: 715 IESLEIDENEGLVKVGVRGCGEMRVFASNEPINCKMEGEDVEFEYDDDDKMVKLQIPWPS 774
Query: 721 SSRLSIIE 727
SS+LSIIE
Sbjct: 775 SSKLSIIE 778
BLAST of MS020004 vs. ExPASy TrEMBL
Match:
A0A6J1E8I6 (galactinol--sucrose galactosyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111431613 PE=3 SV=1)
HSP 1 Score: 1273.8 bits (3295), Expect = 0.0e+00
Identity = 601/731 (82.22%), Postives = 657/731 (89.88%), Query Frame = 0
Query: 1 PSPLISETET----QLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWV 60
PS LI +T L GCF+GFDA E SCH+ IG LRGIRFSSIFRFKVWWTT WV
Sbjct: 92 PSQLIFSHKTTIPKSLLGGCFLGFDAAEPKSCHLVPIGNLRGIRFSSIFRFKVWWTTHWV 151
Query: 61 GTCGSDVQHETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTC 120
G+CG D+QHETQMMILD +DQGRPFVL LPILEGAFRCSLRPG GDD VAMWVESGST
Sbjct: 152 GSCGGDIQHETQMMILDTNDQGRPFVLFLPILEGAFRCSLRPG--GDDNVAMWVESGSTT 211
Query: 121 VRASRFRSCLYVQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFY 180
V+AS+FRSCLY+QVG+DPY+LV + M VKL LGTFKLL++KTPP I+DKFGWCTWDAFY
Sbjct: 212 VQASQFRSCLYMQVGKDPYTLVNEGMNVVKLHLGTFKLLDDKTPPRIVDKFGWCTWDAFY 271
Query: 181 LKVNPQGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMP 240
LKVNP G+ TGVKCLVDGGCPPGM+LIDDGWQSIAHDADSI D QEAMDLT AGEQMP
Sbjct: 272 LKVNPHGVWTGVKCLVDGGCPPGMILIDDGWQSIAHDADSIAD-PHQEAMDLTVAGEQMP 331
Query: 241 CRLIKFEENYKFREYKS-DRENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNV 300
CRLIK+EENYKFR+Y S + + GLGAFV+DLKEEFR++E+VYVWHALCGYWGGIRPNV
Sbjct: 332 CRLIKYEENYKFRDYGSRGKGSGVGLGAFVRDLKEEFRTIEHVYVWHALCGYWGGIRPNV 391
Query: 301 LGMPLSRIISPKLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDG 360
GMPLSR+ISP S+ LEMTMEDLAVDKIV++G+G VPPELAH+MY+GLHSHLQSAGIDG
Sbjct: 392 PGMPLSRVISPNPSKGLEMTMEDLAVDKIVNNGVGFVPPELAHEMYDGLHSHLQSAGIDG 451
Query: 361 VKVDVIHLLEMLSEEFGGRIELAKAYYKALTMSIHKHFKGNGVIASMEHCNDFMYLGTEA 420
VKVDVIHLLEMLSEEFGGRIELAKAYYKALT SI+KH +GNGVIASME CNDFMYLGTEA
Sbjct: 452 VKVDVIHLLEMLSEEFGGRIELAKAYYKALTASINKHLQGNGVIASMEQCNDFMYLGTEA 511
Query: 421 ITLGRVGDDFWTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAA 480
I LGRVGDDFWT D SGDPYWLQGCHMVHCAYNSLWMGN+IHPDWDMFQS HPCAEFHAA
Sbjct: 512 IALGRVGDDFWTVDASGDPYWLQGCHMVHCAYNSLWMGNIIHPDWDMFQSTHPCAEFHAA 571
Query: 481 SRAISGGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILK 540
SRAISGGPIY+SDSVG HNF LLK LVLPDGSILRCQ++ALP+RDCLFEDPL DGKT+LK
Sbjct: 572 SRAISGGPIYVSDSVGKHNFKLLKRLVLPDGSILRCQHYALPSRDCLFEDPLQDGKTMLK 631
Query: 541 IWNLNKFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKG 600
IWNLN+FTGALGLFNCQGGGWCPKTRQNRR SEYARTLTCVAGPKDIEWNNGK PIS+KG
Sbjct: 632 IWNLNQFTGALGLFNCQGGGWCPKTRQNRRTSEYARTLTCVAGPKDIEWNNGKNPISLKG 691
Query: 601 VNFFAIYMVRQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVEFAPIGLADMLN 660
V+ FAIYM+++KKL LLK SENLE++IAPL+Y+LLVVSPVTVLSKPYVEFAPIGL +MLN
Sbjct: 692 VHLFAIYMIQEKKLRLLKTSENLEFTIAPLSYELLVVSPVTVLSKPYVEFAPIGLVNMLN 751
Query: 661 PGGAIQSLEIDENEGLVRVGARGCGEILVFASEGPRSCKIDGEEVGFEYEDDQMVKIQVP 720
GGAIQSLEI+ENEGLVRVGARG GE+ VFAS+ PRSCKIDGE+V FEY DD+MVKIQV
Sbjct: 752 CGGAIQSLEIEENEGLVRVGARGYGEMRVFASKEPRSCKIDGEDVEFEY-DDKMVKIQVT 811
Query: 721 WPGSSRLSIIE 727
WP SSRLSIIE
Sbjct: 812 WPISSRLSIIE 818
BLAST of MS020004 vs. ExPASy TrEMBL
Match:
A0A5D3BJB8 (Galactinol--sucrose galactosyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G003830 PE=3 SV=1)
HSP 1 Score: 1272.7 bits (3292), Expect = 0.0e+00
Identity = 599/728 (82.28%), Postives = 653/728 (89.70%), Query Frame = 0
Query: 1 PSPLISETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCG 60
PSP +T L +GCF+GFDA E S H+ IG L+GIRFSS+FRFKVWWTT WVG+CG
Sbjct: 55 PSPF--KTNNSL-LGCFIGFDATEPKSSHLVPIGNLKGIRFSSLFRFKVWWTTHWVGSCG 114
Query: 61 SDVQHETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRAS 120
D+QHETQMMILD + +GRP+VLLLPILEGAFRCSLR G + DD+VAMWVESGST V AS
Sbjct: 115 RDIQHETQMMILDTNHRGRPYVLLLPILEGAFRCSLRSGDENDDSVAMWVESGSTSVHAS 174
Query: 121 RFRSCLYVQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVN 180
RFRSCLY+QVG+DPYSLVK+AMK VKL LGTFKLL EKTPP I+DKFGWCTWDAFYLKVN
Sbjct: 175 RFRSCLYMQVGDDPYSLVKEAMKVVKLHLGTFKLLHEKTPPPIVDKFGWCTWDAFYLKVN 234
Query: 181 PQGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLI 240
PQGIRTGVKCLVDGGCPPGM+LIDDGWQSIAHDA SITD EAMDLT AGEQMPCRLI
Sbjct: 235 PQGIRTGVKCLVDGGCPPGMILIDDGWQSIAHDAHSITD-HHLEAMDLTAAGEQMPCRLI 294
Query: 241 KFEENYKFREYKSDRENC-KGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMP 300
KFEENYKFR+Y SD + GLGAFVKDLKEEFR++E VYVWHALCGYWGGIRPNV MP
Sbjct: 295 KFEENYKFRDYGSDGKGFGVGLGAFVKDLKEEFRTIENVYVWHALCGYWGGIRPNVPRMP 354
Query: 301 LSRIISPKLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVD 360
LSR++ PKLSQ LE TMEDLAVDKIV++GIGLVPPELAH+MY+GLHSHLQSAGIDGVKVD
Sbjct: 355 LSRVVIPKLSQGLEKTMEDLAVDKIVNNGIGLVPPELAHEMYDGLHSHLQSAGIDGVKVD 414
Query: 361 VIHLLEMLSEEFGGRIELAKAYYKALTMSIHKHFKGNGVIASMEHCNDFMYLGTEAITLG 420
VIHLLEMLSEEFGGRIELAKAYYKALT SI KH +GNG IASMEHCNDFMYLGTEAI LG
Sbjct: 415 VIHLLEMLSEEFGGRIELAKAYYKALTASIKKHLQGNGAIASMEHCNDFMYLGTEAIALG 474
Query: 421 RVGDDFWTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAI 480
RVGDDFWT D SGDPYWLQGCHMVHCAYNSLWMGN+IHPDWDMFQS HPCAEFHAASRAI
Sbjct: 475 RVGDDFWTVDPSGDPYWLQGCHMVHCAYNSLWMGNIIHPDWDMFQSTHPCAEFHAASRAI 534
Query: 481 SGGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKIWNL 540
SGGPIY+SDSVG HNF LLK LVLPDGSILRCQ++ALPTRDCLFEDPLHDGKT+LKIWNL
Sbjct: 535 SGGPIYVSDSVGKHNFKLLKRLVLPDGSILRCQHYALPTRDCLFEDPLHDGKTMLKIWNL 594
Query: 541 NKFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFF 600
NKFTG LGLFNCQGGGWCPKTR+NRR SEYARTLTCVAGPKDIEWNNG++ IS+KGVN F
Sbjct: 595 NKFTGTLGLFNCQGGGWCPKTRKNRRTSEYARTLTCVAGPKDIEWNNGRSLISLKGVNLF 654
Query: 601 AIYMVRQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVEFAPIGLADMLNPGGA 660
AIYMVR KKL+LLK SE+LE++IAPLNY+LLVVSPVTVLSKP +EFAPIGL +MLN GGA
Sbjct: 655 AIYMVRDKKLKLLKTSESLEFTIAPLNYELLVVSPVTVLSKPNMEFAPIGLVNMLNCGGA 714
Query: 661 IQSLEIDENEGLVRVGARGCGEILVFASEGPRSCKIDGEEVGFEY-EDDQMVKIQVPWPG 720
I+SLEIDENEGLV G RGCGE+ VFAS P +CKI+GE+V FEY +DD+MVKIQ+PWP
Sbjct: 715 IESLEIDENEGLVIAGVRGCGEMRVFASNEPINCKIEGEDVEFEYDDDDKMVKIQIPWPS 774
Query: 721 SSRLSIIE 727
SS+LSIIE
Sbjct: 775 SSKLSIIE 778
BLAST of MS020004 vs. ExPASy TrEMBL
Match:
A0A1S3AXR5 (galactinol--sucrose galactosyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103483741 PE=3 SV=1)
HSP 1 Score: 1272.7 bits (3292), Expect = 0.0e+00
Identity = 599/728 (82.28%), Postives = 653/728 (89.70%), Query Frame = 0
Query: 1 PSPLISETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCG 60
PSP +T L +GCF+GFDA E S H+ IG L+GIRFSS+FRFKVWWTT WVG+CG
Sbjct: 55 PSPF--KTNNSL-LGCFIGFDATEPKSSHLVPIGNLKGIRFSSLFRFKVWWTTHWVGSCG 114
Query: 61 SDVQHETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRAS 120
D+QHETQMMILD + +GRP+VLLLPILEGAFRCSLR G + DD+VAMWVESGST V AS
Sbjct: 115 RDIQHETQMMILDTNHRGRPYVLLLPILEGAFRCSLRSGDENDDSVAMWVESGSTSVHAS 174
Query: 121 RFRSCLYVQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVN 180
RFRSCLY+QVG+DPYSLVK+AMK VKL LGTFKLL EKTPP I+DKFGWCTWDAFYLKVN
Sbjct: 175 RFRSCLYMQVGDDPYSLVKEAMKVVKLHLGTFKLLHEKTPPPIVDKFGWCTWDAFYLKVN 234
Query: 181 PQGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLI 240
PQGIRTGVKCLVDGGCPPGM+LIDDGWQSIAHDA SITD EAMDLT AGEQMPCRLI
Sbjct: 235 PQGIRTGVKCLVDGGCPPGMILIDDGWQSIAHDAHSITD-HHLEAMDLTAAGEQMPCRLI 294
Query: 241 KFEENYKFREYKSDRENC-KGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMP 300
KFEENYKFR+Y SD + GLGAFVKDLKEEFR++E VYVWHALCGYWGGIRPNV MP
Sbjct: 295 KFEENYKFRDYGSDGKGFGVGLGAFVKDLKEEFRTIENVYVWHALCGYWGGIRPNVPRMP 354
Query: 301 LSRIISPKLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVD 360
LSR++ PKLSQ LE TMEDLAVDKIV++GIGLVPPELAH+MY+GLHSHLQSAGIDGVKVD
Sbjct: 355 LSRVVIPKLSQGLEKTMEDLAVDKIVNNGIGLVPPELAHEMYDGLHSHLQSAGIDGVKVD 414
Query: 361 VIHLLEMLSEEFGGRIELAKAYYKALTMSIHKHFKGNGVIASMEHCNDFMYLGTEAITLG 420
VIHLLEMLSEEFGGRIELAKAYYKALT SI KH +GNG IASMEHCNDFMYLGTEAI LG
Sbjct: 415 VIHLLEMLSEEFGGRIELAKAYYKALTASIKKHLQGNGAIASMEHCNDFMYLGTEAIALG 474
Query: 421 RVGDDFWTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAI 480
RVGDDFWT D SGDPYWLQGCHMVHCAYNSLWMGN+IHPDWDMFQS HPCAEFHAASRAI
Sbjct: 475 RVGDDFWTVDPSGDPYWLQGCHMVHCAYNSLWMGNIIHPDWDMFQSTHPCAEFHAASRAI 534
Query: 481 SGGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKIWNL 540
SGGPIY+SDSVG HNF LLK LVLPDGSILRCQ++ALPTRDCLFEDPLHDGKT+LKIWNL
Sbjct: 535 SGGPIYVSDSVGKHNFKLLKRLVLPDGSILRCQHYALPTRDCLFEDPLHDGKTMLKIWNL 594
Query: 541 NKFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFF 600
NKFTG LGLFNCQGGGWCPKTR+NRR SEYARTLTCVAGPKDIEWNNG++ IS+KGVN F
Sbjct: 595 NKFTGTLGLFNCQGGGWCPKTRKNRRTSEYARTLTCVAGPKDIEWNNGRSLISLKGVNLF 654
Query: 601 AIYMVRQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVEFAPIGLADMLNPGGA 660
AIYMVR KKL+LLK SE+LE++IAPLNY+LLVVSPVTVLSKP +EFAPIGL +MLN GGA
Sbjct: 655 AIYMVRDKKLKLLKTSESLEFTIAPLNYELLVVSPVTVLSKPNMEFAPIGLVNMLNCGGA 714
Query: 661 IQSLEIDENEGLVRVGARGCGEILVFASEGPRSCKIDGEEVGFEY-EDDQMVKIQVPWPG 720
I+SLEIDENEGLV G RGCGE+ VFAS P +CKI+GE+V FEY +DD+MVKIQ+PWP
Sbjct: 715 IESLEIDENEGLVIAGVRGCGEMRVFASNEPINCKIEGEDVEFEYDDDDKMVKIQIPWPS 774
Query: 721 SSRLSIIE 727
SS+LSIIE
Sbjct: 775 SSKLSIIE 778
BLAST of MS020004 vs. TAIR 10
Match:
AT5G40390.1 (Raffinose synthase family protein )
HSP 1 Score: 999.2 bits (2582), Expect = 1.7e-291
Identity = 469/728 (64.42%), Postives = 571/728 (78.43%), Query Frame = 0
Query: 12 LAVGCFVGFDAD-ESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCGSDVQHETQMM 71
++ G F+GF+ D E S HVASIG+L+ IRF SIFRFKVWWTT WVG+ G D+++ETQ++
Sbjct: 62 VSAGSFIGFNLDGEPKSHHVASIGKLKNIRFMSIFRFKVWWTTHWVGSNGRDIENETQII 121
Query: 72 ILDR--------SDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRASRF 131
ILD+ S GRP+VLLLP+LEG+FR S + G DD VA+ VESGST V S F
Sbjct: 122 ILDQSGSDSGPGSGSGRPYVLLLPLLEGSFRSSFQSG--EDDDVAVCVESGSTEVTGSEF 181
Query: 132 RSCLYVQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVNPQ 191
R +YV G+DP+ LVKDAMK +++ + TFKLLEEK+PPGI+DKFGWCTWDAFYL VNP
Sbjct: 182 RQIVYVHAGDDPFKLVKDAMKVIRVHMNTFKLLEEKSPPGIVDKFGWCTWDAFYLTVNPD 241
Query: 192 GIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLIKF 251
G+ GVKCLVDGGCPPG+VLIDDGWQSI HD+D I + E M++T AGEQMPCRL+KF
Sbjct: 242 GVHKGVKCLVDGGCPPGLVLIDDGWQSIGHDSDGI----DVEGMNITVAGEQMPCRLLKF 301
Query: 252 EENYKFREYKSDR-ENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMPLS 311
EEN+KF++Y S + +N G+ AFV+DLK+EF +V+Y+YVWHALCGYWGG+RP +P S
Sbjct: 302 EENHKFKDYVSPKDQNDVGMKAFVRDLKDEFSTVDYIYVWHALCGYWGGLRPEAPALPPS 361
Query: 312 RIISPKLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVDVI 371
II P+LS L++TMEDLAVDKI+ +GIG P+LA + YEGLHSHLQ+AGIDGVKVDVI
Sbjct: 362 TIIRPELSPGLKLTMEDLAVDKIIETGIGFASPDLAKEFYEGLHSHLQNAGIDGVKVDVI 421
Query: 372 HLLEMLSEEFGGRIELAKAYYKALTMSIHKHFKGNGVIASMEHCNDFMYLGTEAITLGRV 431
H+LEML +++GGR++LAKAY+KALT S++KHF GNGVIASMEHCNDFM+LGTEAI+LGRV
Sbjct: 422 HILEMLCQKYGGRVDLAKAYFKALTSSVNKHFNGNGVIASMEHCNDFMFLGTEAISLGRV 481
Query: 432 GDDFWTKDLSGDP---YWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRA 491
GDDFW D SGDP +WLQGCHMVHCAYNSLWMGN I PDWDMFQS HPCAEFHAASRA
Sbjct: 482 GDDFWCTDPSGDPNGTFWLQGCHMVHCAYNSLWMGNFIQPDWDMFQSTHPCAEFHAASRA 541
Query: 492 ISGGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKIWN 551
ISGGPIYISD VG H+F+LLK LVLP+GSILRC+Y+ALPTRD LFEDPLHDGKT+LKIWN
Sbjct: 542 ISGGPIYISDCVGKHDFDLLKRLVLPNGSILRCEYYALPTRDRLFEDPLHDGKTMLKIWN 601
Query: 552 LNKFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNF 611
LNK+TG +G FNCQGGGWC +TR+N+ SE TLT PKD+EWN+G +PIS+ V
Sbjct: 602 LNKYTGVIGAFNCQGGGWCRETRRNQCFSECVNTLTATTSPKDVEWNSGSSPISIANVEE 661
Query: 612 FAIYMVRQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVEFAPIGLADMLNPGG 671
FA+++ + KKL L +++LE ++ P ++L+ VSPV + V FAPIGL +MLN G
Sbjct: 662 FALFLSQSKKLLLSGLNDDLELTLEPFKFELITVSPVVTIEGNSVRFAPIGLVNMLNTSG 721
Query: 672 AIQSLEIDENEGLVRVGARGCGEILVFASEGPRSCKIDGEEVGFEYEDDQMVKIQVPWPG 727
AI+SL N+ V VG G GE V+AS+ P SC IDGE V F YE D MV +QVPW G
Sbjct: 722 AIRSLVY--NDESVEVGVFGAGEFRVYASKKPVSCLIDGEVVEFGYE-DSMVMVQVPWSG 780
BLAST of MS020004 vs. TAIR 10
Match:
AT4G01970.1 (stachyose synthase )
HSP 1 Score: 662.5 bits (1708), Expect = 3.8e-190
Identity = 343/801 (42.82%), Postives = 474/801 (59.18%), Query Frame = 0
Query: 1 PSPLISETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCG 60
P P++ + G F+GF + + S+GR F S+FRFK+WW+T W+G G
Sbjct: 72 PLPILLRVQANAHKGGFLGFTKESPSDRLTNSLGRFEDREFLSLFRFKMWWSTAWIGKSG 131
Query: 61 SDVQHETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRAS 120
SD+Q ETQ ++L + + +V ++P +EGAFR SL PG G+ V + ESGST V+ S
Sbjct: 132 SDLQAETQWVML-KIPEIDSYVAIIPTIEGAFRASLTPGEKGN--VLICAESGSTKVKES 191
Query: 121 RFRSCLYVQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVN 180
F+S Y+ + ++PY+L+K+A A+++ + TFKLLEEK P I+DKFGWCTWDA YL V+
Sbjct: 192 SFKSIAYIHICDNPYNLMKEAFSALRVHMNTFKLLEEKKLPKIVDKFGWCTWDACYLTVD 251
Query: 181 PQGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLI 240
P I TGVK DGG P V+IDDGWQSI D D + +++A +L GEQM RL
Sbjct: 252 PATIWTGVKEFEDGGVCPKFVIIDDGWQSINFDGDEL----DKDAENLVLGGEQMTARLT 311
Query: 241 KFEENYKFREYK------------------------------------------------ 300
F+E KFR YK
Sbjct: 312 SFKECKKFRNYKGGSFITSDASHFNPLKPKMLIYKATERIQAIILRRKLVKESGEQDLTE 371
Query: 301 --------------------------SDRENCKGLGAFVKDLKEEFRSVEYVYVWHALCG 360
SD + G+ AF KDL+ F+S++ +YVWHALCG
Sbjct: 372 LDEKIKILSEELNAMFDEVEKEESLGSDDVSGSGMAAFTKDLRLRFKSLDDIYVWHALCG 431
Query: 361 YWGGIRPNVLGMPLSRIISP-KLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLH 420
W G+RP + M L ++P +LS L TM DLAVDK+V +GIGLV P AH+ Y+ +H
Sbjct: 432 AWNGVRPETM-MDLKAKVAPFELSPSLGATMADLAVDKVVEAGIGLVHPSKAHEFYDSMH 491
Query: 421 SHLQSAGIDGVKVDVIHLLEMLSEEFGGRIELAKAYYKALTMSIHKHFKGNGVIASMEHC 480
S+L S G+ G K+DV LE L+EE GGR+ELAKAYY LT S+ K+F G VIASM+ C
Sbjct: 492 SYLASVGVTGAKIDVFQTLESLAEEHGGRVELAKAYYDGLTESMIKNFNGTDVIASMQQC 551
Query: 481 NDFMYLGTEAITLGRVGDDFWTKDLSGDP---YWLQGCHMVHCAYNSLWMGNMIHPDWDM 540
N+F +L T+ I++GRVGDDFW +D GDP YWLQG HM+HC+YNS+WMG MI PDWDM
Sbjct: 552 NEFFFLATKQISIGRVGDDFWWQDPYGDPQGVYWLQGVHMIHCSYNSIWMGQMIQPDWDM 611
Query: 541 FQSNHPCAEFHAASRAISGGPIYISDSVG--SHNFNLLKSLVLPDGSILRCQYHALPTRD 600
FQS+H CAE+HAASRAI GGP+Y+SD +G SHNF+L+K L DG+I RC ++ALPTRD
Sbjct: 612 FQSDHVCAEYHAASRAICGGPVYLSDHLGKASHNFDLIKKLAFFDGTIPRCVHYALPTRD 671
Query: 601 CLFEDPLHDGKTILKIWNLNKFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPK 660
LF++PL D ++ILKI+N NKF G +G FNCQG GW P+ + + E T++
Sbjct: 672 SLFKNPLFDKESILKIFNFNKFGGVIGTFNCQGAGWSPEEHRFKGYKECYTTVSGTVHVS 731
Query: 661 DIEWNNGKTPISVKGVNFFAIYMV-RQKKLELL---KASENLEYSIAPLNYQLLVVSPVT 717
DIEW+ + V + Y+V +Q+ E+L SE ++ ++ P + LL PVT
Sbjct: 732 DIEWDQNPEAAGSQ-VTYTGDYLVYKQQSEEILFMNSKSEAMKITLEPSAFDLLSFVPVT 791
BLAST of MS020004 vs. TAIR 10
Match:
AT5G20250.1 (Raffinose synthase family protein )
HSP 1 Score: 545.0 bits (1403), Expect = 8.9e-155
Identity = 285/715 (39.86%), Postives = 411/715 (57.48%), Query Frame = 0
Query: 6 SETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCGSDVQH 65
S +E G FVG ++ S H+ IG LR RF S FRFK+WW Q +G G D+ +
Sbjct: 33 SASEAGPVEGVFVGAVFNKEESKHIVPIGTLRNSRFMSCFRFKLWWMAQRMGEMGRDIPY 92
Query: 66 ETQMMILDRSD--------------QGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVE 125
ETQ ++++ +D + + + LP++EG+FR L+ G+ +D V + +E
Sbjct: 93 ETQFLLVESNDGSHLESDGANGVECNQKVYTVFLPLIEGSFRSCLQ--GNVNDEVELCLE 152
Query: 126 SGSTCVRASRFRSCLYVQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCT 185
SG + S F LY+ G DP+ + DA++ VKL L +F+ EK PGI+D FGWCT
Sbjct: 153 SGDVDTKRSSFTHSLYIHAGTDPFQTITDAIRTVKLHLNSFRQRHEKKLPGIVDYFGWCT 212
Query: 186 WDAFYLKVNPQGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTA 245
WDAFY +V +G+ G+K L GG PP V+IDDGWQS+ DA ++ G E++
Sbjct: 213 WDAFYQEVTQEGVEAGLKSLAAGGTPPKFVIIDDGWQSVERDA-TVEAGDEKK------- 272
Query: 246 GEQMPCRLIKFEENYKFREYKSDRENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGI 305
E RL +EN KF+ K D N G+ VK KE+ ++YVYVWHA+ GYWGG+
Sbjct: 273 -ESPIFRLTGIKENEKFK--KKDDPNV-GIKNIVKIAKEK-HGLKYVYVWHAITGYWGGV 332
Query: 306 RPNVLGMPLSRIIS-PKLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQS 365
RP G ++ P +S+ + D + G+GLV P+ +K Y LHS+L
Sbjct: 333 RP---GEEYGSVMKYPNMSKGVVENDPTWKTDVMTLQGLGLVSPKKVYKFYNELHSYLAD 392
Query: 366 AGIDGVKVDVIHLLEMLSEEFGGRIELAKAYYKALTMSIHKHFKGNGVIASMEHCNDFMY 425
AG+DGVKVDV +LE L GGR+EL + +++AL S+ K+F NG IA M H D +Y
Sbjct: 393 AGVDGVKVDVQCVLETLGGGLGGRVELTRQFHQALDSSVAKNFPDNGCIACMSHNTDALY 452
Query: 426 LGTEAITLGRVGDDFWTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCA 485
+A + R DDF+ +D H+ AYNS+++G + PDWDMF S HP A
Sbjct: 453 CSKQAAVI-RASDDFYPRDPVS-----HTIHIASVAYNSVFLGEFMQPDWDMFHSVHPAA 512
Query: 486 EFHAASRAISGGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDG 545
E+HA++RAISGGP+Y+SDS G HNF LL+ LVLPDGSILR + PTRDCLF DP DG
Sbjct: 513 EYHASARAISGGPLYVSDSPGKHNFELLRKLVLPDGSILRARLPGRPTRDCLFADPARDG 572
Query: 546 KTILKIWNLNKFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTP 605
++LKIWN+NK+TG LG++NCQG W R+N +LT +D+ + +
Sbjct: 573 VSLLKIWNMNKYTGVLGVYNCQGAAWSSTERKNIFHQTKTDSLTGSIRGRDVHSISEAST 632
Query: 606 ISVKGVNFFAIYMVRQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVEFAPIGL 665
A+Y + +L ++ + +L S+ +++ VSP++ L V FAPIGL
Sbjct: 633 DPTTWNGDCAVYSQSRGELIVMPYNVSLPVSLKIREHEIFTVSPISHLVDG-VSFAPIGL 692
Query: 666 ADMLNPGGAIQSLEIDENEGLVRVGARGCGEILVFASEGPRSCKIDGEEVGFEYE 706
+M N GGAI+ L + + V + +GCG+ ++S P+ C ++ E+ FEY+
Sbjct: 693 VNMYNSGGAIEGLRYEAEKMKVVMEVKGCGKFGSYSSVKPKRCVVESNEIAFEYD 722
BLAST of MS020004 vs. TAIR 10
Match:
AT5G20250.2 (Raffinose synthase family protein )
HSP 1 Score: 545.0 bits (1403), Expect = 8.9e-155
Identity = 285/715 (39.86%), Postives = 411/715 (57.48%), Query Frame = 0
Query: 6 SETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCGSDVQH 65
S +E G FVG ++ S H+ IG LR RF S FRFK+WW Q +G G D+ +
Sbjct: 33 SASEAGPVEGVFVGAVFNKEESKHIVPIGTLRNSRFMSCFRFKLWWMAQRMGEMGRDIPY 92
Query: 66 ETQMMILDRSD--------------QGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVE 125
ETQ ++++ +D + + + LP++EG+FR L+ G+ +D V + +E
Sbjct: 93 ETQFLLVESNDGSHLESDGANGVECNQKVYTVFLPLIEGSFRSCLQ--GNVNDEVELCLE 152
Query: 126 SGSTCVRASRFRSCLYVQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCT 185
SG + S F LY+ G DP+ + DA++ VKL L +F+ EK PGI+D FGWCT
Sbjct: 153 SGDVDTKRSSFTHSLYIHAGTDPFQTITDAIRTVKLHLNSFRQRHEKKLPGIVDYFGWCT 212
Query: 186 WDAFYLKVNPQGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTA 245
WDAFY +V +G+ G+K L GG PP V+IDDGWQS+ DA ++ G E++
Sbjct: 213 WDAFYQEVTQEGVEAGLKSLAAGGTPPKFVIIDDGWQSVERDA-TVEAGDEKK------- 272
Query: 246 GEQMPCRLIKFEENYKFREYKSDRENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGI 305
E RL +EN KF+ K D N G+ VK KE+ ++YVYVWHA+ GYWGG+
Sbjct: 273 -ESPIFRLTGIKENEKFK--KKDDPNV-GIKNIVKIAKEK-HGLKYVYVWHAITGYWGGV 332
Query: 306 RPNVLGMPLSRIIS-PKLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQS 365
RP G ++ P +S+ + D + G+GLV P+ +K Y LHS+L
Sbjct: 333 RP---GEEYGSVMKYPNMSKGVVENDPTWKTDVMTLQGLGLVSPKKVYKFYNELHSYLAD 392
Query: 366 AGIDGVKVDVIHLLEMLSEEFGGRIELAKAYYKALTMSIHKHFKGNGVIASMEHCNDFMY 425
AG+DGVKVDV +LE L GGR+EL + +++AL S+ K+F NG IA M H D +Y
Sbjct: 393 AGVDGVKVDVQCVLETLGGGLGGRVELTRQFHQALDSSVAKNFPDNGCIACMSHNTDALY 452
Query: 426 LGTEAITLGRVGDDFWTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCA 485
+A + R DDF+ +D H+ AYNS+++G + PDWDMF S HP A
Sbjct: 453 CSKQAAVI-RASDDFYPRDPVS-----HTIHIASVAYNSVFLGEFMQPDWDMFHSVHPAA 512
Query: 486 EFHAASRAISGGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDG 545
E+HA++RAISGGP+Y+SDS G HNF LL+ LVLPDGSILR + PTRDCLF DP DG
Sbjct: 513 EYHASARAISGGPLYVSDSPGKHNFELLRKLVLPDGSILRARLPGRPTRDCLFADPARDG 572
Query: 546 KTILKIWNLNKFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTP 605
++LKIWN+NK+TG LG++NCQG W R+N +LT +D+ + +
Sbjct: 573 VSLLKIWNMNKYTGVLGVYNCQGAAWSSTERKNIFHQTKTDSLTGSIRGRDVHSISEAST 632
Query: 606 ISVKGVNFFAIYMVRQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVEFAPIGL 665
A+Y + +L ++ + +L S+ +++ VSP++ L V FAPIGL
Sbjct: 633 DPTTWNGDCAVYSQSRGELIVMPYNVSLPVSLKIREHEIFTVSPISHLVDG-VSFAPIGL 692
Query: 666 ADMLNPGGAIQSLEIDENEGLVRVGARGCGEILVFASEGPRSCKIDGEEVGFEYE 706
+M N GGAI+ L + + V + +GCG+ ++S P+ C ++ E+ FEY+
Sbjct: 693 VNMYNSGGAIEGLRYEAEKMKVVMEVKGCGKFGSYSSVKPKRCVVESNEIAFEYD 722
BLAST of MS020004 vs. TAIR 10
Match:
AT5G20250.3 (Raffinose synthase family protein )
HSP 1 Score: 545.0 bits (1403), Expect = 8.9e-155
Identity = 285/715 (39.86%), Postives = 411/715 (57.48%), Query Frame = 0
Query: 6 SETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCGSDVQH 65
S +E G FVG ++ S H+ IG LR RF S FRFK+WW Q +G G D+ +
Sbjct: 33 SASEAGPVEGVFVGAVFNKEESKHIVPIGTLRNSRFMSCFRFKLWWMAQRMGEMGRDIPY 92
Query: 66 ETQMMILDRSD--------------QGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVE 125
ETQ ++++ +D + + + LP++EG+FR L+ G+ +D V + +E
Sbjct: 93 ETQFLLVESNDGSHLESDGANGVECNQKVYTVFLPLIEGSFRSCLQ--GNVNDEVELCLE 152
Query: 126 SGSTCVRASRFRSCLYVQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCT 185
SG + S F LY+ G DP+ + DA++ VKL L +F+ EK PGI+D FGWCT
Sbjct: 153 SGDVDTKRSSFTHSLYIHAGTDPFQTITDAIRTVKLHLNSFRQRHEKKLPGIVDYFGWCT 212
Query: 186 WDAFYLKVNPQGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTA 245
WDAFY +V +G+ G+K L GG PP V+IDDGWQS+ DA ++ G E++
Sbjct: 213 WDAFYQEVTQEGVEAGLKSLAAGGTPPKFVIIDDGWQSVERDA-TVEAGDEKK------- 272
Query: 246 GEQMPCRLIKFEENYKFREYKSDRENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGI 305
E RL +EN KF+ K D N G+ VK KE+ ++YVYVWHA+ GYWGG+
Sbjct: 273 -ESPIFRLTGIKENEKFK--KKDDPNV-GIKNIVKIAKEK-HGLKYVYVWHAITGYWGGV 332
Query: 306 RPNVLGMPLSRIIS-PKLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQS 365
RP G ++ P +S+ + D + G+GLV P+ +K Y LHS+L
Sbjct: 333 RP---GEEYGSVMKYPNMSKGVVENDPTWKTDVMTLQGLGLVSPKKVYKFYNELHSYLAD 392
Query: 366 AGIDGVKVDVIHLLEMLSEEFGGRIELAKAYYKALTMSIHKHFKGNGVIASMEHCNDFMY 425
AG+DGVKVDV +LE L GGR+EL + +++AL S+ K+F NG IA M H D +Y
Sbjct: 393 AGVDGVKVDVQCVLETLGGGLGGRVELTRQFHQALDSSVAKNFPDNGCIACMSHNTDALY 452
Query: 426 LGTEAITLGRVGDDFWTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCA 485
+A + R DDF+ +D H+ AYNS+++G + PDWDMF S HP A
Sbjct: 453 CSKQAAVI-RASDDFYPRDPVS-----HTIHIASVAYNSVFLGEFMQPDWDMFHSVHPAA 512
Query: 486 EFHAASRAISGGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDG 545
E+HA++RAISGGP+Y+SDS G HNF LL+ LVLPDGSILR + PTRDCLF DP DG
Sbjct: 513 EYHASARAISGGPLYVSDSPGKHNFELLRKLVLPDGSILRARLPGRPTRDCLFADPARDG 572
Query: 546 KTILKIWNLNKFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTP 605
++LKIWN+NK+TG LG++NCQG W R+N +LT +D+ + +
Sbjct: 573 VSLLKIWNMNKYTGVLGVYNCQGAAWSSTERKNIFHQTKTDSLTGSIRGRDVHSISEAST 632
Query: 606 ISVKGVNFFAIYMVRQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVEFAPIGL 665
A+Y + +L ++ + +L S+ +++ VSP++ L V FAPIGL
Sbjct: 633 DPTTWNGDCAVYSQSRGELIVMPYNVSLPVSLKIREHEIFTVSPISHLVDG-VSFAPIGL 692
Query: 666 ADMLNPGGAIQSLEIDENEGLVRVGARGCGEILVFASEGPRSCKIDGEEVGFEYE 706
+M N GGAI+ L + + V + +GCG+ ++S P+ C ++ E+ FEY+
Sbjct: 693 VNMYNSGGAIEGLRYEAEKMKVVMEVKGCGKFGSYSSVKPKRCVVESNEIAFEYD 722
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022137045.1 | 0.0e+00 | 98.90 | galactinol--sucrose galactosyltransferase-like [Momordica charantia] | [more] |
| XP_038894030.1 | 0.0e+00 | 82.76 | galactinol--sucrose galactosyltransferase-like [Benincasa hispida] | [more] |
| XP_023519339.1 | 0.0e+00 | 82.94 | galactinol--sucrose galactosyltransferase-like [Cucurbita pepo subsp. pepo] | [more] |
| KAG7019802.1 | 0.0e+00 | 82.35 | Galactinol--sucrose galactosyltransferase, partial [Cucurbita argyrosperma subsp... | [more] |
| XP_004152562.2 | 0.0e+00 | 81.73 | LOW QUALITY PROTEIN: galactinol--sucrose galactosyltransferase [Cucumis sativus] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q8VWN6 | 0.0e+00 | 69.04 | Galactinol--sucrose galactosyltransferase OS=Pisum sativum OX=3888 GN=RFS PE=1 S... | [more] |
| Q9FND9 | 2.4e-290 | 64.42 | Probable galactinol--sucrose galactosyltransferase 5 OS=Arabidopsis thaliana OX=... | [more] |
| Q5VQG4 | 2.2e-275 | 61.13 | Galactinol--sucrose galactosyltransferase OS=Oryza sativa subsp. japonica OX=399... | [more] |
| Q93XK2 | 2.7e-196 | 43.76 | Stachyose synthase OS=Pisum sativum OX=3888 GN=STS1 PE=1 SV=1 | [more] |
| Q9SYJ4 | 5.4e-189 | 42.82 | Probable galactinol--sucrose galactosyltransferase 4 OS=Arabidopsis thaliana OX=... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1C5K5 | 0.0e+00 | 98.90 | galactinol--sucrose galactosyltransferase-like OS=Momordica charantia OX=3673 GN... | [more] |
| A0A0A0LWD8 | 0.0e+00 | 81.73 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G046280 PE=3 SV=1 | [more] |
| A0A6J1E8I6 | 0.0e+00 | 82.22 | galactinol--sucrose galactosyltransferase-like OS=Cucurbita moschata OX=3662 GN=... | [more] |
| A0A5D3BJB8 | 0.0e+00 | 82.28 | Galactinol--sucrose galactosyltransferase-like OS=Cucumis melo var. makuwa OX=11... | [more] |
| A0A1S3AXR5 | 0.0e+00 | 82.28 | galactinol--sucrose galactosyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103... | [more] |
