MS019989 (gene) Bitter gourd (TR) v1

Overview


Name	MS019989
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Non-specific serine/threonine protein kinase
Location	scaffold22: 1040129 .. 1043741 (+)
RNA-Seq Expression	MS019989
Synteny	MS019989

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCAGGAATTGTCATTGGATCAAGACCTGGACGAATCTGATCCCGAGTTCGTGGAGATTGATCCTACTGGTCGATATGGACGGGTAAATACTCATGCAGTTAATAGTTTATCTTGTTTTGCATTTCTAGTTGACATTTTATTTTGGTACTGTAATTGCTCACGTTGATACTTGGCTGAACCTTTTTTCTCCTTGGTTGGAACTTGTATAAAATGCTGAGATGGCCATTGAACTTCCAGATTTCAGAATATTTACCAGGACGGGGGGTTATTCGTTGTCTTATTAATGTTTTGCCATTTCTCCTAATCTCTATTTGTTTTGCAGTATAAGGAAATTTTAGGAAAAGGGGCGTACAAAAAAGTGTATCCTTTTTCGCTTCCATGTTAGTGGTGATGCAATTAAACATGTAATTTCGTGCATTTTCTATTTTAAACTGATTCTATCTAGGAAATTAAAATTTGCGCATCTGCCTGCGTTTGGTTTGGGATGAAACTTTGGAGTAAATTGTGTGTTCTTGATCAAGATGTGGTATATAGATATCGGGCTTTTGATGAGTTGGAAGGAATTGAAGTAGCTTGGAATCAGATTAAGGTAACAGATGTACTGCGGAACTCTGAAGATTTGGAGCGACTCTATTCAGAAGTTCATTTGCTCAAGACATTGAAGCACAAGAACATAATCAAATTTTACAATTCATGGGTTGATACGAAAAATGAAAACATCAACTTCATTACCGAGATATTCACTTCAGGGACACTACGGCAGTGAGTCTTTTGCCCTGGCCTCTTCAGATTTTGTTTTTCTTCTTTAATTATACTTAAATATATATTATGAACACATGTAGATATAGAAAGAAACACAAGCATGTTGATTTGAGGGCTCTGAAAAAGTGGTCCAGGCAGATATTAGAGGGCCTTCTGTATCTTCATAGTCATGACCCTCCAGTTATACATAGAGATTTAAAATGTGACAATATCTTTGTCAATGGCAACCAAGGTGAGGTGAAAATCGGTGACCTGGGACTTGCTGCCATTCTTCAGCAGGCTCGTTCGGCTCACAGTGTTATTGGTGAGGATTTTAAACTTGAATAAGCCATTATGATAGCTCTTATTCAATATCGAAGTTTATGGATAAATGTTTTCAGTGGAGGCAGTAAGATGACTTACTCTGTGTCTGTGATTTAATGATTTTAGGGACACCGGAGTTTATGGCGCCAGAGCTGTACGAGGAGGAATATAATGAGCTTGTGGATATTTATGCCTTCGGCATGTGTTTGCTTGAGCTTGTAACCTTTGAATATCCATATGTCGAATGTGCAAATGCTGCTCAAATATATAAGAAGGTCTCATCAGTAAGGGCTCCTGGATCATTTTTCCTTTTGTATCCCATGCAGTACCTTGATACTAATATTTCTTGTTTATTTCTTAACCCTATTTCTGGCATTTATGTATGATGCCTTTGGAACTGACTGCTGAAAAGGGCTGTCAGGTTGTGGGGCTGCCCTACCAGTTCTATCACCTTCTAATGAAGCGTGAATACTGTATGAATAATGCTACTTTATTAGATTTTGTTAGGACTCAAAACACCATATCATATAGGAAATAGTCTATGATTTAGTTTCATCGTGTACATTGTGCAACCTGGACTTTTCTATCAAACCTTATATTTATCGTGATGCATTTGACCTACCATTCACTTTAAGATTTCATTCAGCTGTGGTAACTTATAGTTTATGACAAAGAAGCAGCAGTATTCTTGTTGTCTATTCAATCTTCAAAGCTTGATCCATATGATTGTTATGTGTGGTCTGCAATTGTTATCTCCAGGCCAGGTAAATAGTTTTCGGAATGGTTTCCCAGATGCTATATAAAGAATTACGTTCATGGAATCTAGGCTTCCTAGTGCATTTTTCTAGGCATTTAAACAAGGAGCTTTACGTGTGTTTTCATGTGATGTGTATCATTTTGTGCAAGTTTCTTCTTCCTTCTTTTTCCTCGCTTCAATTTTAGAATGAACCACCACTTGTTTAAAGCCCCGGCTCTTATTTTGGCATCAAATTTGTTTTAGTTCGGATTGAAATTTTGTAATGTTGTATCAGATAAATGATAGCTGTGTATGTACTTCAATTTGGTAAATACATGGGAGTCCTAAAATGCTTTAGAAAATTTATATAATCCAAACGCTGCTAATTTCTCAAATCAGATACTGTATGTCCAGATTTGATCTGTATTGTTTTTTAACCCTCTTATGCAGGTTCTTTAAATATAAGGTTCCTTGAGAAAGCTTTTAAGGAATTATTTTTTTCCTTTTGTTCCTAGGGGATAAAGCCAGCTTCTTTGGCAAAAGTCACCAATCCTGGAGTTAAAGCACTTATAGAAAAATGTATTGCAAATGTCTCTGACCGCCTACCTGCAAAAGACCTTTTAAGGGATCCATTTCTCCTAGCAGATGATGATCATGAAAGAATAACTCGTCACTTAAAAAACAAGACACAACACACAGGTAAGTTTAAGAAAATACATCTCGCTCAGTCAAAAAATTCTGGGATTGTAACTTACTGTTTTTTTACTGGACATATGCCCATGACAGAAGAAAAGAGAGAGCAGCTCCATATCGATAGATGTACAGATGATACTTCTGCTGAGACTACCAGAGACTTCAGCATGCATGGTCAGAGAAAAGATGTTAACAAGATATTTCTCAAATTACGAATTGCTGACGCCTTGGGTAAAATTCATATTTCTCTGTCATTTCCTTCTAGTTCTTTTAACTTCTTTAGAAGAATAGCTACTCTATTGGAATTTCCAGTATTCCGTTTACAAGTTCTCCAATTATTTTAGGTAATGTCCGCAACATCCATTTCCCGTTTGATATTGAAGCCGACACAGCAATTTCTGTTGCAAGTGAAATGGTCGAGGAATTGGACCTTTCAGATCAAGATGTCTCCACAATTTCTGAAATGATCGAAACTGAAATCCGGTCCTACATTCCAGACTGGACATCAGTTGAGTATGGTGTAGATAATGTAGGGGGAGATGTTGCAATTTCTGATAGCTCTCCATCTGAAACGAGGAATGGTGCCTCTCCCTTGTCTATTGAATCTGGTAACCTTGCATTAGAGATAATGCCTTCAGGTCGTAAGTACTGGTCTGACTCTCCAAAGGGAATTGGTGGGTGCTCTCCTGTTAAGCCAGGTCCTTCAAACTTGTCTTGTATGGATCAGAATGTTGAATCTTCTGGCAGCCACATGCCTAGGGATAATCTAGATCATGCTGCTATAATTAAGGGACTGGAAAGTGAGCTTTTGTCTGATTATGGCGGTAATGGCGGGCAGGATGAGGGTAGCTCAACTACAAATCTTCATGCTAGCTCTGATGCACACCCTTCAGAAGAGAACTGTGGAGTTTCAGAACGTGATGAATCAGAATTCATCGAGGTAATGGCTGAAAAACTTGATAAACTGTTGATACAGCAGCAAAAGGAATTAGATGAGCTAAAGAGGGAGCATAAATTAGTTATTTCAGAACTTTTGAGTGAACTTACACCTGAGTCCTATGAGAAGGTTTTAAGGATATGCAAGCTAGAGCCTTCTGATTATGACTCGGGTCTC

mRNA sequence

ATGTCTCAGGAATTGTCATTGGATCAAGACCTGGACGAATCTGATCCCGAGTTCGTGGAGATTGATCCTACTGGTCGATATGGACGGTATAAGGAAATTTTAGGAAAAGGGGCGTACAAAAAAGTATATCGGGCTTTTGATGAGTTGGAAGGAATTGAAGTAGCTTGGAATCAGATTAAGGTAACAGATGTACTGCGGAACTCTGAAGATTTGGAGCGACTCTATTCAGAAGTTCATTTGCTCAAGACATTGAAGCACAAGAACATAATCAAATTTTACAATTCATGGGTTGATACGAAAAATGAAAACATCAACTTCATTACCGAGATATTCACTTCAGGGACACTACGGCAATATAGAAAGAAACACAAGCATGTTGATTTGAGGGCTCTGAAAAAGTGGTCCAGGCAGATATTAGAGGGCCTTCTGTATCTTCATAGTCATGACCCTCCAGTTATACATAGAGATTTAAAATGTGACAATATCTTTGTCAATGGCAACCAAGGTGAGGTGAAAATCGGTGACCTGGGACTTGCTGCCATTCTTCAGCAGGCTCGTTCGGCTCACAGTGTTATTGGGACACCGGAGTTTATGGCGCCAGAGCTGTACGAGGAGGAATATAATGAGCTTGTGGATATTTATGCCTTCGGCATGTGTTTGCTTGAGCTTGTAACCTTTGAATATCCATATGTCGAATGTGCAAATGCTGCTCAAATATATAAGAAGGTCTCATCAGGGATAAAGCCAGCTTCTTTGGCAAAAGTCACCAATCCTGGAGTTAAAGCACTTATAGAAAAATGTATTGCAAATGTCTCTGACCGCCTACCTGCAAAAGACCTTTTAAGGGATCCATTTCTCCTAGCAGATGATGATCATGAAAGAATAACTCGTCACTTAAAAAACAAGACACAACACACAGAAAAGAGAGAGCAGCTCCATATCGATAGATGTACAGATGATACTTCTGCTGAGACTACCAGAGACTTCAGCATGCATGGTCAGAGAAAAGATGTTAACAAGATATTTCTCAAATTACGAATTGCTGACGCCTTGGGTAATGTCCGCAACATCCATTTCCCGTTTGATATTGAAGCCGACACAGCAATTTCTGTTGCAAGTGAAATGGTCGAGGAATTGGACCTTTCAGATCAAGATGTCTCCACAATTTCTGAAATGATCGAAACTGAAATCCGGTCCTACATTCCAGACTGGACATCAGTTGAGTATGGTGTAGATAATGTAGGGGGAGATGTTGCAATTTCTGATAGCTCTCCATCTGAAACGAGGAATGGTGCCTCTCCCTTGTCTATTGAATCTGGTAACCTTGCATTAGAGATAATGCCTTCAGGTCGTAAGTACTGGTCTGACTCTCCAAAGGGAATTGGTGGGTGCTCTCCTGTTAAGCCAGGTCCTTCAAACTTGTCTTGTATGGATCAGAATGTTGAATCTTCTGGCAGCCACATGCCTAGGGATAATCTAGATCATGCTGCTATAATTAAGGGACTGGAAAGTGAGCTTTTGTCTGATTATGGCGGTAATGGCGGGCAGGATGAGGGTAGCTCAACTACAAATCTTCATGCTAGCTCTGATGCACACCCTTCAGAAGAGAACTGTGGAGTTTCAGAACGTGATGAATCAGAATTCATCGAGGTAATGGCTGAAAAACTTGATAAACTGTTGATACAGCAGCAAAAGGAATTAGATGAGCTAAAGAGGGAGCATAAATTAGTTATTTCAGAACTTTTGAGTGAACTTACACCTGAGTCCTATGAGAAGGTTTTAAGGATATGCAAGCTAGAGCCTTCTGATTATGACTCGGGTCTC

Coding sequence (CDS)

ATGTCTCAGGAATTGTCATTGGATCAAGACCTGGACGAATCTGATCCCGAGTTCGTGGAGATTGATCCTACTGGTCGATATGGACGGTATAAGGAAATTTTAGGAAAAGGGGCGTACAAAAAAGTATATCGGGCTTTTGATGAGTTGGAAGGAATTGAAGTAGCTTGGAATCAGATTAAGGTAACAGATGTACTGCGGAACTCTGAAGATTTGGAGCGACTCTATTCAGAAGTTCATTTGCTCAAGACATTGAAGCACAAGAACATAATCAAATTTTACAATTCATGGGTTGATACGAAAAATGAAAACATCAACTTCATTACCGAGATATTCACTTCAGGGACACTACGGCAATATAGAAAGAAACACAAGCATGTTGATTTGAGGGCTCTGAAAAAGTGGTCCAGGCAGATATTAGAGGGCCTTCTGTATCTTCATAGTCATGACCCTCCAGTTATACATAGAGATTTAAAATGTGACAATATCTTTGTCAATGGCAACCAAGGTGAGGTGAAAATCGGTGACCTGGGACTTGCTGCCATTCTTCAGCAGGCTCGTTCGGCTCACAGTGTTATTGGGACACCGGAGTTTATGGCGCCAGAGCTGTACGAGGAGGAATATAATGAGCTTGTGGATATTTATGCCTTCGGCATGTGTTTGCTTGAGCTTGTAACCTTTGAATATCCATATGTCGAATGTGCAAATGCTGCTCAAATATATAAGAAGGTCTCATCAGGGATAAAGCCAGCTTCTTTGGCAAAAGTCACCAATCCTGGAGTTAAAGCACTTATAGAAAAATGTATTGCAAATGTCTCTGACCGCCTACCTGCAAAAGACCTTTTAAGGGATCCATTTCTCCTAGCAGATGATGATCATGAAAGAATAACTCGTCACTTAAAAAACAAGACACAACACACAGAAAAGAGAGAGCAGCTCCATATCGATAGATGTACAGATGATACTTCTGCTGAGACTACCAGAGACTTCAGCATGCATGGTCAGAGAAAAGATGTTAACAAGATATTTCTCAAATTACGAATTGCTGACGCCTTGGGTAATGTCCGCAACATCCATTTCCCGTTTGATATTGAAGCCGACACAGCAATTTCTGTTGCAAGTGAAATGGTCGAGGAATTGGACCTTTCAGATCAAGATGTCTCCACAATTTCTGAAATGATCGAAACTGAAATCCGGTCCTACATTCCAGACTGGACATCAGTTGAGTATGGTGTAGATAATGTAGGGGGAGATGTTGCAATTTCTGATAGCTCTCCATCTGAAACGAGGAATGGTGCCTCTCCCTTGTCTATTGAATCTGGTAACCTTGCATTAGAGATAATGCCTTCAGGTCGTAAGTACTGGTCTGACTCTCCAAAGGGAATTGGTGGGTGCTCTCCTGTTAAGCCAGGTCCTTCAAACTTGTCTTGTATGGATCAGAATGTTGAATCTTCTGGCAGCCACATGCCTAGGGATAATCTAGATCATGCTGCTATAATTAAGGGACTGGAAAGTGAGCTTTTGTCTGATTATGGCGGTAATGGCGGGCAGGATGAGGGTAGCTCAACTACAAATCTTCATGCTAGCTCTGATGCACACCCTTCAGAAGAGAACTGTGGAGTTTCAGAACGTGATGAATCAGAATTCATCGAGGTAATGGCTGAAAAACTTGATAAACTGTTGATACAGCAGCAAAAGGAATTAGATGAGCTAAAGAGGGAGCATAAATTAGTTATTTCAGAACTTTTGAGTGAACTTACACCTGAGTCCTATGAGAAGGTTTTAAGGATATGCAAGCTAGAGCCTTCTGATTATGACTCGGGTCTC

Protein sequence

MSQELSLDQDLDESDPEFVEIDPTGRYGRYKEILGKGAYKKVYRAFDELEGIEVAWNQIKVTDVLRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYRKKHKHVDLRALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYVECANAAQIYKKVSSGIKPASLAKVTNPGVKALIEKCIANVSDRLPAKDLLRDPFLLADDDHERITRHLKNKTQHTEKREQLHIDRCTDDTSAETTRDFSMHGQRKDVNKIFLKLRIADALGNVRNIHFPFDIEADTAISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDWTSVEYGVDNVGGDVAISDSSPSETRNGASPLSIESGNLALEIMPSGRKYWSDSPKGIGGCSPVKPGPSNLSCMDQNVESSGSHMPRDNLDHAAIIKGLESELLSDYGGNGGQDEGSSTTNLHASSDAHPSEENCGVSERDESEFIEVMAEKLDKLLIQQQKELDELKREHKLVISELLSELTPESYEKVLRICKLEPSDYDSGL

Homology

[Close]

BLAST of MS019989 vs. NCBI nr
Match: XP_022137111.1 (probable serine/threonine-protein kinase WNK3 [Momordica charantia])

HSP 1 Score: 1192.6 bits (3084), Expect = 0.0e+00
Identity = 608/609 (99.84%), Postives = 608/609 (99.84%), Query Frame = 0


Query: 1   MSQELSLDQDLDESDPEFVEIDPTGRYGRYKEILGKGAYKKVYRAFDELEGIEVAWNQIK 60
           MSQELSLDQDLDESDPEFVEIDPTGRYGRYKEILGKGAYKKVYRAFDELEGIEVAWNQIK
Sbjct: 1   MSQELSLDQDLDESDPEFVEIDPTGRYGRYKEILGKGAYKKVYRAFDELEGIEVAWNQIK 60

Query: 61  VTDVLRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR 120
           VTDVLRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR
Sbjct: 61  VTDVLRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR 120

Query: 121 KKHKHVDLRALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA 180
           KKHKHVDLRALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA
Sbjct: 121 KKHKHVDLRALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA 180

Query: 181 ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYVECANAAQIY 240
           ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYVECANAAQIY
Sbjct: 181 ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYVECANAAQIY 240

Query: 241 KKVSSGIKPASLAKVTNPGVKALIEKCIANVSDRLPAKDLLRDPFLLADDDHERITRHLK 300
           KKVSSGIKPASLAKVTNPGVKALIEKCIANVSDRLPAKDLLRDPFLLADDDHERITRHLK
Sbjct: 241 KKVSSGIKPASLAKVTNPGVKALIEKCIANVSDRLPAKDLLRDPFLLADDDHERITRHLK 300

Query: 301 NKTQHT-EKREQLHIDRCTDDTSAETTRDFSMHGQRKDVNKIFLKLRIADALGNVRNIHF 360
           NKTQHT EKREQLHIDRCTDDTSAETTRDFSMHGQRKDVNKIFLKLRIADALGNVRNIHF
Sbjct: 301 NKTQHTEEKREQLHIDRCTDDTSAETTRDFSMHGQRKDVNKIFLKLRIADALGNVRNIHF 360

Query: 361 PFDIEADTAISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDWTSVEYGVDNVGGDVA 420
           PFDIEADTAISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDWTSVEYGVDNVGGDVA
Sbjct: 361 PFDIEADTAISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDWTSVEYGVDNVGGDVA 420

Query: 421 ISDSSPSETRNGASPLSIESGNLALEIMPSGRKYWSDSPKGIGGCSPVKPGPSNLSCMDQ 480
           ISDSSPSETRNGASPLSIESGNLALEIMPSGRKYWSDSPKGIGGCSPVKPGPSNLSCMDQ
Sbjct: 421 ISDSSPSETRNGASPLSIESGNLALEIMPSGRKYWSDSPKGIGGCSPVKPGPSNLSCMDQ 480

Query: 481 NVESSGSHMPRDNLDHAAIIKGLESELLSDYGGNGGQDEGSSTTNLHASSDAHPSEENCG 540
           NVESSGSHMPRDNLDHAAIIKGLESELLSDYGGNGGQDEGSSTTNLHASSDAHPSEENCG
Sbjct: 481 NVESSGSHMPRDNLDHAAIIKGLESELLSDYGGNGGQDEGSSTTNLHASSDAHPSEENCG 540

Query: 541 VSERDESEFIEVMAEKLDKLLIQQQKELDELKREHKLVISELLSELTPESYEKVLRICKL 600
           VSERDESEFIEVMAEKLDKLLIQQQKELDELKREHKLVISELLSELTPESYEKVLRICKL
Sbjct: 541 VSERDESEFIEVMAEKLDKLLIQQQKELDELKREHKLVISELLSELTPESYEKVLRICKL 600

Query: 601 EPSDYDSGL 609
           EPSDYDSGL
Sbjct: 601 EPSDYDSGL 609

[Close]

BLAST of MS019989 vs. NCBI nr
Match: XP_038894587.1 (probable serine/threonine-protein kinase WNK3 [Benincasa hispida])

HSP 1 Score: 1010.4 bits (2611), Expect = 6.7e-291
Identity = 517/606 (85.31%), Postives = 561/606 (92.57%), Query Frame = 0


Query: 1   MSQELSLDQDLDESDPEFVEIDPTGRYGRYKEILGKGAYKKVYRAFDELEGIEVAWNQIK 60
           MSQ+LSLDQDLDESDPEFVEIDPTGRYGRYKEILGKGA+K+VYRAFDELEGIEVAWNQ+K
Sbjct: 1   MSQDLSLDQDLDESDPEFVEIDPTGRYGRYKEILGKGAFKRVYRAFDELEGIEVAWNQVK 60

Query: 61  VTDVLRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR 120
           VTD+LRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR
Sbjct: 61  VTDLLRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR 120

Query: 121 KKHKHVDLRALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA 180
           KKHKHVDLRALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA
Sbjct: 121 KKHKHVDLRALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA 180

Query: 181 ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYVECANAAQIY 240
           ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPY+ECANAAQIY
Sbjct: 181 ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYIECANAAQIY 240

Query: 241 KKVSSGIKPASLAKVTNPGVKALIEKCIANVSDRLPAKDLLRDPFLLADDDHERITRHLK 300
           KKV+SGIKPASLAKVT+ GV+  IEKCIANVSDRLPAKDLLRDPFL ADDDHE I+RHL+
Sbjct: 241 KKVTSGIKPASLAKVTDLGVRTFIEKCIANVSDRLPAKDLLRDPFLRADDDHESISRHLQ 300

Query: 301 NKTQHTEKREQLHIDRCTDDTSAETTRDFSMHGQRKDVNKIFLKLRIADALGNVRNIHFP 360
           +K  HTEKR+ + IDR  DD SAET+RDFSMHG+RKDVNKIFLKLRIAD++GN RNIHFP
Sbjct: 301 SKLLHTEKRDHVDIDRSVDDASAETSRDFSMHGERKDVNKIFLKLRIADSMGNFRNIHFP 360

Query: 361 FDIEADTAISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDWTSVEYGVDNVGGDVAI 420
           FDIEADTAISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDW SVEY +DNVG DVAI
Sbjct: 361 FDIEADTAISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDWISVEYSIDNVGADVAI 420

Query: 421 SDSSPSETRNGASPLSIESGNLALEIMPSGRKYWSDSPKGIGGCSPVKPGPSNLS-CMDQ 480
           SDSSPSETRN ASPLSIESGNLALE+MPSGRKYWSDSPKGIGG SP KPGPSNLS  +D 
Sbjct: 421 SDSSPSETRNAASPLSIESGNLALEVMPSGRKYWSDSPKGIGGSSPNKPGPSNLSFTLDH 480

Query: 481 NVESSGSHMPRDNLDHAAIIKGLESELLSDYGGNGGQDEGSSTTNLHASSDAHPSEENCG 540
           NVESS SH+  DNLDHAAIIKGLESELLS+ G + GQDEGSSTTN+HA+S+   SEEN  
Sbjct: 481 NVESS-SHLHGDNLDHAAIIKGLESELLSEGGDHDGQDEGSSTTNIHANSETRLSEEN-- 540

Query: 541 VSERDESEFIEVMAEKLDKLLIQQQKELDELKREHKLVISELLSELTPESYEKVLRICKL 600
               DES  ++++ E+L+ LL QQQKELDELK++HKL ISELL++LTPESY+KV+ +C+L
Sbjct: 541 --NYDESVELKIVVEELENLLAQQQKELDELKKKHKLAISELLTKLTPESYQKVVEMCQL 600

Query: 601 EPSDYD 606
           + SD++
Sbjct: 601 KHSDFE 601

[Close]

BLAST of MS019989 vs. NCBI nr
Match: XP_011651790.1 (probable serine/threonine-protein kinase WNK3 [Cucumis sativus] >XP_031740994.1 probable serine/threonine-protein kinase WNK3 [Cucumis sativus] >XP_031740996.1 probable serine/threonine-protein kinase WNK3 [Cucumis sativus] >XP_031740998.1 probable serine/threonine-protein kinase WNK3 [Cucumis sativus] >XP_031741000.1 probable serine/threonine-protein kinase WNK3 [Cucumis sativus] >XP_031741005.1 probable serine/threonine-protein kinase WNK3 [Cucumis sativus] >XP_031741006.1 probable serine/threonine-protein kinase WNK3 [Cucumis sativus] >XP_031741007.1 probable serine/threonine-protein kinase WNK3 [Cucumis sativus] >XP_031741009.1 probable serine/threonine-protein kinase WNK3 [Cucumis sativus] >XP_031741010.1 probable serine/threonine-protein kinase WNK3 [Cucumis sativus] >XP_031741016.1 probable serine/threonine-protein kinase WNK3 [Cucumis sativus] >XP_031741018.1 probable serine/threonine-protein kinase WNK3 [Cucumis sativus] >KGN64347.1 hypothetical protein Csa_014235 [Cucumis sativus])

HSP 1 Score: 998.0 bits (2579), Expect = 3.4e-287
Identity = 510/606 (84.16%), Postives = 555/606 (91.58%), Query Frame = 0


Query: 1   MSQELSLDQDLDESDPEFVEIDPTGRYGRYKEILGKGAYKKVYRAFDELEGIEVAWNQIK 60
           MSQ+LS DQDLDESDPEFVEIDPTGRYGRYKEILGKGA+K+VYRAFDELEGIEVAWNQ+K
Sbjct: 1   MSQDLSPDQDLDESDPEFVEIDPTGRYGRYKEILGKGAFKRVYRAFDELEGIEVAWNQVK 60

Query: 61  VTDVLRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR 120
           VTD+LRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR
Sbjct: 61  VTDLLRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR 120

Query: 121 KKHKHVDLRALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA 180
           KKHKHVD+RALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA
Sbjct: 121 KKHKHVDVRALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA 180

Query: 181 ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYVECANAAQIY 240
           ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPY+ECANAAQIY
Sbjct: 181 ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYIECANAAQIY 240

Query: 241 KKVSSGIKPASLAKVTNPGVKALIEKCIANVSDRLPAKDLLRDPFLLADDDHERITRHLK 300
           KKV+SGIKPASLAKVTN GV+A IEKCIANVSDRLPAKDLLRDPFL ADDDHE I+RHL+
Sbjct: 241 KKVTSGIKPASLAKVTNLGVRAFIEKCIANVSDRLPAKDLLRDPFLQADDDHESISRHLR 300

Query: 301 NKTQHTEKREQLHIDRCTDDTSAETTRDFSMHGQRKDVNKIFLKLRIADALGNVRNIHFP 360
           +KTQ TEK+EQ+  DR  D + AET+RDFSMHG+RKDVNKIFLKLRIAD++GN RNIHFP
Sbjct: 301 SKTQPTEKKEQIDFDRSVDYSPAETSRDFSMHGERKDVNKIFLKLRIADSMGNFRNIHFP 360

Query: 361 FDIEADTAISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDWTSVEYGVDNVGGDVAI 420
           FDIEADTAISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDW SVEY  DNVG D  +
Sbjct: 361 FDIEADTAISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDWISVEYSGDNVGADAPV 420

Query: 421 SDSSPSETRNGASPLSIESGNLALEIMPSGRKYWSDSPKGIGGCSPVKPGPSNLS-CMDQ 480
           SDSSPSETRN ASPLSIESGNLALE+MPSGRKYWSDSPKGIGGCSP+KPGPSNLS   DQ
Sbjct: 421 SDSSPSETRNVASPLSIESGNLALEVMPSGRKYWSDSPKGIGGCSPIKPGPSNLSFASDQ 480

Query: 481 NVESSGSHMPRDNLDHAAIIKGLESELLSDYGGNGGQDEGSSTTNLHASSDAHPSEENCG 540
           NVESS SH+  DNLDHAAIIKGLE+ELLS+ G + GQDE S    +H SS+ H SEEN  
Sbjct: 481 NVESSNSHIHGDNLDHAAIIKGLENELLSEGGDHDGQDESS----IHTSSETHHSEEN-- 540

Query: 541 VSERDESEFIEVMAEKLDKLLIQQQKELDELKREHKLVISELLSELTPESYEKVLRICKL 600
               DES  ++++AEKL+ LL QQQKELDEL+++HKL ISELL++LTPESY+KV+ +C+L
Sbjct: 541 --NYDESVDLKIVAEKLENLLTQQQKELDELRKKHKLDISELLTKLTPESYQKVIEMCQL 598

Query: 601 EPSDYD 606
           +  D++
Sbjct: 601 QHPDFE 598

[Close]

BLAST of MS019989 vs. NCBI nr
Match: XP_008438930.1 (PREDICTED: probable serine/threonine-protein kinase WNK3 [Cucumis melo])

HSP 1 Score: 995.3 bits (2572), Expect = 2.2e-286
Identity = 513/606 (84.65%), Postives = 556/606 (91.75%), Query Frame = 0


Query: 1   MSQELSLDQDLDESDPEFVEIDPTGRYGRYKEILGKGAYKKVYRAFDELEGIEVAWNQIK 60
           MSQ+L LDQDLDESDPEFVEIDPTGRYGRYKEILGKGA+K+VYRAFDELEGIEVAWNQ+K
Sbjct: 1   MSQDLPLDQDLDESDPEFVEIDPTGRYGRYKEILGKGAFKRVYRAFDELEGIEVAWNQVK 60

Query: 61  VTDVLRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR 120
           VTD+LRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR
Sbjct: 61  VTDLLRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR 120

Query: 121 KKHKHVDLRALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA 180
           KKHKHVDLRALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA
Sbjct: 121 KKHKHVDLRALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA 180

Query: 181 ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYVECANAAQIY 240
           ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYVECANAAQIY
Sbjct: 181 ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYVECANAAQIY 240

Query: 241 KKVSSGIKPASLAKVTNPGVKALIEKCIANVSDRLPAKDLLRDPFLLADDDHERITRHLK 300
           KKV+SGIKPASLAKVTN GVK  IEKCIANVSDRL AKDLLRDPFL ADDDH+ I+RHL+
Sbjct: 241 KKVTSGIKPASLAKVTNLGVKTFIEKCIANVSDRLSAKDLLRDPFLQADDDHDSISRHLQ 300

Query: 301 NKTQHTEKREQLHIDRCTDDTSAETTRDFSMHGQRKDVNKIFLKLRIADALGNVRNIHFP 360
           + T+HT KREQ+  DR  D + AET+RDFSMHG+RKDVNKIFLKLRIAD++GN RNIHFP
Sbjct: 301 SSTRHTVKREQIDTDRSIDYSPAETSRDFSMHGERKDVNKIFLKLRIADSMGNFRNIHFP 360

Query: 361 FDIEADTAISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDWTSVEYGVDNVGGDVAI 420
           FDIEADTAISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDW SVEY VDNVG DVAI
Sbjct: 361 FDIEADTAISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDWISVEYSVDNVGADVAI 420

Query: 421 SDSSPSETRNGASPLSIESGNLALEIMPSGRKYWSDSPKGIGGCSPVKPGPSNLS-CMDQ 480
           SDSSPSETRN ASPLSIESGNLALE+MPSGRKYWSDSPKGIGGCSP+KPGPSNLS   D 
Sbjct: 421 SDSSPSETRNVASPLSIESGNLALEVMPSGRKYWSDSPKGIGGCSPIKPGPSNLSFTTDL 480

Query: 481 NVESSGSHMPRDNLDHAAIIKGLESELLSDYGGNGGQDEGSSTTNLHASSDAHPSEENCG 540
           NVESS SH+  DNLDHAAIIKGLE+ELLS+ G + GQD GSS   +H SS+ H SEEN  
Sbjct: 481 NVESSNSHVHGDNLDHAAIIKGLENELLSEGGDHDGQD-GSS---IHTSSETHYSEEN-- 540

Query: 541 VSERDESEFIEVMAEKLDKLLIQQQKELDELKREHKLVISELLSELTPESYEKVLRICKL 600
               DES  ++++AEKL+ LL QQQKELDEL+++HKL ISELL++LTPESY+KV+++C+L
Sbjct: 541 --NYDESVDLKIVAEKLENLLTQQQKELDELRKKHKLAISELLTKLTPESYQKVIKMCQL 598

Query: 601 EPSDYD 606
           +  D++
Sbjct: 601 KRPDFE 598

[Close]

BLAST of MS019989 vs. NCBI nr
Match: XP_023542613.1 (probable serine/threonine-protein kinase WNK3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 992.6 bits (2565), Expect = 1.4e-285
Identity = 509/604 (84.27%), Postives = 550/604 (91.06%), Query Frame = 0


Query: 1   MSQELSLDQDLDESDPEFVEIDPTGRYGRYKEILGKGAYKKVYRAFDELEGIEVAWNQIK 60
           MSQ+LSLDQD DESDPEFVEIDPTGRYGRYKEILGKGA+KKVYRAFDELEGIEVAWNQ+K
Sbjct: 1   MSQDLSLDQDPDESDPEFVEIDPTGRYGRYKEILGKGAFKKVYRAFDELEGIEVAWNQVK 60

Query: 61  VTDVLRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR 120
           VTD+LRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR
Sbjct: 61  VTDLLRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR 120

Query: 121 KKHKHVDLRALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA 180
           KKHKHVDLRALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA
Sbjct: 121 KKHKHVDLRALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA 180

Query: 181 ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYVECANAAQIY 240
           ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPY+ECANAAQIY
Sbjct: 181 ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYIECANAAQIY 240

Query: 241 KKVSSGIKPASLAKVTNPGVKALIEKCIANVSDRLPAKDLLRDPFLLADDDHERITRHLK 300
           KKV+SGIKPASLAKVT+ GV+A IEKCIANVSDRLPAKDLLRDPFLLAD+DHE I+RHL 
Sbjct: 241 KKVTSGIKPASLAKVTDGGVRAFIEKCIANVSDRLPAKDLLRDPFLLADEDHESISRHLH 300

Query: 301 NKTQHTEKREQLHIDRCTDDTSAETTRDFSMHGQRKDVNKIFLKLRIADALGNVRNIHFP 360
            K  H EKREQ+  D   DDT A T+ DFSMHGQRKDVNKIFLKLRIAD++GN RNIHFP
Sbjct: 301 PKIHHKEKREQIDTDTSVDDTPA-TSVDFSMHGQRKDVNKIFLKLRIADSMGNFRNIHFP 360

Query: 361 FDIEADTAISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDWTSVEYGVDNVGGDVAI 420
           FDIEADT+ISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDWTSVEY VDNVG +V I
Sbjct: 361 FDIEADTSISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDWTSVEYAVDNVGAEVVI 420

Query: 421 SDSSPSETRNGASPLSIESGNLALEIMPSGRKYWSDSPKGIGGCSPVKPGPSNLS-CMDQ 480
           SDSSPS++R  ASP+SIESGNLALE++PSGRKYWSDSPKG+GGCSPVKPGPSNLS   +Q
Sbjct: 421 SDSSPSDSRTAASPISIESGNLALEVLPSGRKYWSDSPKGVGGCSPVKPGPSNLSFASEQ 480

Query: 481 NVESSGSHMPRDNLDHAAIIKGLESELLSDYGGNGGQDEGSSTTNLHASSDAHPSEENCG 540
           NVESS SHM  DN+DHAAII GLE+ELLS+   N GQDE   TT++HASS+AHP      
Sbjct: 481 NVESSSSHMHEDNIDHAAIIMGLENELLSEDAANDGQDESILTTDIHASSEAHP------ 540

Query: 541 VSERDESEFIEVMAEKLDKLLIQQQKELDELKREHKLVISELLSELTPESYEKVLRICKL 600
            S+ DES  IE+M EKL+ LL QQ++ELDELK + KL ISELL+ELTPE+++KVL+ICKL
Sbjct: 541 -SDYDESIGIEIMTEKLENLLTQQKEELDELKTKQKLAISELLAELTPETHQKVLKICKL 596

Query: 601 EPSD 604
           + SD
Sbjct: 601 KHSD 596

[Close]

BLAST of MS019989 vs. ExPASy Swiss-Prot
Match: Q65X23 (Probable serine/threonine-protein kinase WNK2 OS=Oryza sativa subsp. japonica OX=39947 GN=WNK2 PE=2 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 3.6e-170
Identity = 352/640 (55.00%), Postives = 429/640 (67.03%), Query Frame = 0


Query: 10  DLDESDPEFVEIDPTGRYGRYKEILGKGAYKKVYRAFDELEGIEVAWNQIKVTDVLRNSE 69
           DL +++PEF E+DPT RYGRY E+LGKGA+K VY+AFD+LEG+EVAWNQIKV D+LRN++
Sbjct: 10  DLLDTEPEFAEVDPTARYGRYTEVLGKGAFKTVYKAFDQLEGLEVAWNQIKVGDILRNND 69

Query: 70  DLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYRKKHKHVDLR 129
           DLERL SEV LLKTLKHKNIIKFYNSW+D KN NINFITE+FTSGTLRQYR KHK VD+R
Sbjct: 70  DLERLRSEVRLLKTLKHKNIIKFYNSWLDKKNNNINFITEVFTSGTLRQYRIKHKKVDVR 129

Query: 130 ALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAILQQARSAH 189
           ALKKWSRQIL GL+YLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLA IL  ARSAH
Sbjct: 130 ALKKWSRQILSGLVYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLATILDNARSAH 189

Query: 190 SVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYVECANAAQIYKKVSSGIKP 249
           S+IGTPEFMAPELY+EEYNELVDIYAFGMCLLELVTFEYPY EC+NAAQIYKKVS G KP
Sbjct: 190 SIIGTPEFMAPELYDEEYNELVDIYAFGMCLLELVTFEYPYCECSNAAQIYKKVSDGEKP 249

Query: 250 ASLAKVTNPGVKALIEKCIANVSDRLPAKDLLRDPFLLADDDHERITRHLKNKT------ 309
           +SLAK+ +P V+  IEKCIA  S RL A++LL DPFL   DD E+I   L++ T      
Sbjct: 250 SSLAKIEDPEVRFFIEKCIAKASQRLSAQELLMDPFL--RDDGEKIFYPLQSNTKASDGA 309

Query: 310 ---------------------QHTEKREQLH-IDR---CTDDTSAETTRDFSMHGQRKDV 369
                                +HT    + H  DR    T D  A   R  ++  Q KD+
Sbjct: 310 GSSNSSMGYKYDRDASSMAIREHTGSFAEEHPSDRYIHSTMDPQAAAGRIITVESQMKDL 369

Query: 370 NKIFLKLRIADALGNVRNIHFPFDIEADTAISVASEMVEELDLSDQDVSTISEMIETEIR 429
           N IFLKLRIAD+ G+ +NIHFPFDIEADT+ISVA+EMV +LDL+DQDV+ I+EMI+ EIR
Sbjct: 370 NTIFLKLRIADSTGHAQNIHFPFDIEADTSISVATEMVVQLDLTDQDVTAIAEMIDAEIR 429

Query: 430 SYIPDWTSVEYGVDNVGGDVAISDSSPSETRNGASPLSIE----SGNLALEIMPSGRKYW 489
           ++IPDW ++E  V+N G + A S++  SE  +  S L  E          E +PSG KYW
Sbjct: 430 AHIPDW-ALEESVENQGDERAHSETDSSEADDETSELRNEPNATHNGFVQEHLPSGHKYW 489

Query: 490 SDSPKGIGGCSPVKPGPSNLSCMDQNVESS-GSHMPRDNLDHAAIIKGLESELLSDYGGN 549
           SDSP+             N+      VE   G +MP        I+K             
Sbjct: 490 SDSPR------------RNIEMSHSAVEPHIGGNMPN------GILK------------- 549

Query: 550 GGQDEGSSTTNLHASSD-AHPS--EENCGVS------ERDESEFIEV-MAEKLDKLLIQQ 604
              D   + +NL  S D  +PS  +   GV+      +  + E IE  ++E+L  LL QQ
Sbjct: 550 -KNDTDDTVSNLGTSVDLPNPSMIDRKSGVASVSTSPQSFDDEHIEADVSERLVNLLAQQ 609

[Close]

BLAST of MS019989 vs. ExPASy Swiss-Prot
Match: Q9STK6 (Probable serine/threonine-protein kinase WNK3 OS=Arabidopsis thaliana OX=3702 GN=WNK3 PE=2 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 2.6e-165
Identity = 333/599 (55.59%), Postives = 403/599 (67.28%), Query Frame = 0


Query: 9   QDLDESDPEFVEIDPTGRYGRYKEILGKGAYKKVYRAFDELEGIEVAWNQIKVTDVLRNS 68
           QD + S+ EFVEIDPTGRYGRYKE+LGKGA+K+VYRAFD+LEGIEVAWNQ+K+ D   +S
Sbjct: 3   QDENNSEEEFVEIDPTGRYGRYKEVLGKGAFKEVYRAFDQLEGIEVAWNQVKLDDKFCSS 62

Query: 69  EDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYRKKHKHVDL 128
           EDL+RLYSEVHLLKTLKHK+IIKFY SW+D ++  IN ITE+FTSG LRQYRKKHK VDL
Sbjct: 63  EDLDRLYSEVHLLKTLKHKSIIKFYTSWIDHQHMTINLITEVFTSGNLRQYRKKHKCVDL 122

Query: 129 RALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAILQQARSA 188
           RALKKWSRQILEGL+YLHSHDPPVIHRDLKCDNIF+NGNQGEVKIGDLGLAAIL +ARSA
Sbjct: 123 RALKKWSRQILEGLVYLHSHDPPVIHRDLKCDNIFINGNQGEVKIGDLGLAAILHRARSA 182

Query: 189 HSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYVECANAAQIYKKVSSGIK 248
           HSVIGTPEFMAPELYEE+YN LVDIYAFGMCLLELVTFEYPY EC NAAQIY+KV+SGIK
Sbjct: 183 HSVIGTPEFMAPELYEEDYNVLVDIYAFGMCLLELVTFEYPYSECTNAAQIYRKVTSGIK 242

Query: 249 PASLAKVTNPGVKALIEKCIANVSDRLPAKDLLRDPFLLA-DDDHERITRHLKNKTQHTE 308
           PA+L  VT+P V+A IEKCIA VS RL AK+LL DPFL    ++ E ++ H +N      
Sbjct: 243 PAALLNVTDPQVRAFIEKCIAKVSQRLSAKELLDDPFLKCYKENTENVSSHKENGYNGNG 302

Query: 309 KREQLHIDRCTDDTSAETTRDFSMHGQRKDVNKIFLKLRIADALGNVRNIHFPFDIEADT 368
                 +D+ +D      T    + GQRKD+N IFLKLRI D+ G +RNIHFPF+IE DT
Sbjct: 303 -----IVDKLSDSEVGLLT----VEGQRKDLNTIFLKLRITDSKGQIRNIHFPFNIETDT 362

Query: 369 AISVASEMVEELDLS-DQDVSTISEMIETEIRSYIPDWTSVEYGVDNVGGDVAISD--SS 428
           + SVA EMVEELDL+ DQD+STI++MI+TEI S+IPDWT        +G D A+    SS
Sbjct: 363 SFSVAIEMVEELDLTDDQDISTIAKMIDTEIHSHIPDWTPSRL----IGDDSAVQKCLSS 422

Query: 429 PSETRNGASPLSIESGNLALEIMPSGRKYWSDSPKGIGGCSPVKPGPSNLSCMDQNVESS 488
           P                L L+  PSGRK+WS    G G                   +S 
Sbjct: 423 PE--------------TLHLDRFPSGRKFWSSPKAGAG-------------------DSR 482

Query: 489 GSHMPRDNLDHAAIIKGLESELLSDYGGNGGQDEGSSTTNLHASSDAHPSEENCGVSERD 548
               PR N           S+L S  G                     P  +  G     
Sbjct: 483 SPFAPRSN-----------SKLSSAQG---------------------PINQEVG----- 512

Query: 549 ESEFIEVMAEKLDKLLIQQQKELDELKREHKLVISELLSELTPESYEKVLRICKLEPSD 604
                 V+ EKL+ LL +Q++E++E++R+ + +++E L E  PE  E+ L   +++ SD
Sbjct: 543 ------VIVEKLESLLRKQREEIEEMQRDQERIVTEFLKEFPPEICEEALVRLQVKDSD 512

[Close]

BLAST of MS019989 vs. ExPASy Swiss-Prot
Match: A2YMV6 (Probable serine/threonine-protein kinase WNK1 OS=Oryza sativa subsp. indica OX=39946 GN=WNK1 PE=2 SV=2)

HSP 1 Score: 485.7 bits (1249), Expect = 7.5e-136
Identity = 250/427 (58.55%), Postives = 310/427 (72.60%), Query Frame = 0


Query: 16  PEFVEIDPTGRYGRYKEILGKGAYKKVYRAFDELEGIEVAWNQIKVTDVLRNSEDLERLY 75
           PE+ E+DPTGRYGRY ++LGKGA K VYRAFDE +G+EVAWNQ+K+ D L++ EDLERLY
Sbjct: 15  PEYAEVDPTGRYGRYNDVLGKGASKTVYRAFDEYQGMEVAWNQVKLHDFLQSPEDLERLY 74

Query: 76  SEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYRKKHKHVDLRALKKWS 135
            E+HLLKTLKH+NI+KFY SWVD    NINFITE+FTSGTLRQYR+KH  V++ A+K W 
Sbjct: 75  CEIHLLKTLKHRNIMKFYTSWVDVSRRNINFITEMFTSGTLRQYRQKHMRVNIWAVKHWC 134

Query: 136 RQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAILQQARSAHSVIGTP 195
           RQIL GLLYLHSHDPP+IHRDLKCDNIFVNGNQGEVKIGDLGLAAIL+++ + H V GTP
Sbjct: 135 RQILSGLLYLHSHDPPIIHRDLKCDNIFVNGNQGEVKIGDLGLAAILRKSHAVHCV-GTP 194

Query: 196 EFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYVECANAAQIYKKVSSGIKPASLAKV 255
           EFMAPE+YEEEYNELVDIY+FGMC+LE+VTFEYPY EC +  QIYKKV SG KP +L KV
Sbjct: 195 EFMAPEVYEEEYNELVDIYSFGMCVLEMVTFEYPYSECTHPVQIYKKVISGTKPEALYKV 254

Query: 256 TNPGVKALIEKCIANVSDRLPAKDLLRDPFLLADD--------DHERITRHLKNKTQHTE 315
            +P V+  +EKC+A  S RL A++LL+DPFL  DD        D+  +    +   +H  
Sbjct: 255 KDPMVRQFVEKCLATASRRLSARELLKDPFLQVDDLVFCPGDGDYSLMNYLRQPYLEHAY 314

Query: 316 KREQL-------HIDRCT----------DDTSAETTRDFSMH-------------GQRKD 375
               +        ID  T          DD  A+    F+ H             G++ +
Sbjct: 315 SNVSMMSNGLSESIDEDTPTEDRWDCEDDDIKADGIDLFNGHEDEPLGNVDITIKGRKSE 374

Query: 376 VNKIFLKLRIADALGNVRNIHFPFDIEADTAISVASEMVEELDLSDQDVSTISEMIETEI 405
              IFL+LRIAD  G+VRNI+FPFDIEADTA+SVA+EMV ELD++D +V+ I+EMI+ E+
Sbjct: 375 DGSIFLRLRIADNDGHVRNIYFPFDIEADTALSVATEMVAELDITDHEVTRIAEMIDGEV 434

[Close]

BLAST of MS019989 vs. ExPASy Swiss-Prot
Match: Q0D598 (Probable serine/threonine-protein kinase WNK1 OS=Oryza sativa subsp. japonica OX=39947 GN=WNK1 PE=2 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 7.5e-136
Identity = 250/427 (58.55%), Postives = 310/427 (72.60%), Query Frame = 0


Query: 16  PEFVEIDPTGRYGRYKEILGKGAYKKVYRAFDELEGIEVAWNQIKVTDVLRNSEDLERLY 75
           PE+ E+DPTGRYGRY ++LGKGA K VYRAFDE +G+EVAWNQ+K+ D L++ EDLERLY
Sbjct: 15  PEYAEVDPTGRYGRYNDVLGKGASKTVYRAFDEYQGMEVAWNQVKLHDFLQSPEDLERLY 74

Query: 76  SEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYRKKHKHVDLRALKKWS 135
            E+HLLKTLKH+NI+KFY SWVD    NINFITE+FTSGTLRQYR+KH  V++ A+K W 
Sbjct: 75  CEIHLLKTLKHRNIMKFYTSWVDVSRRNINFITEMFTSGTLRQYRQKHMRVNIWAVKHWC 134

Query: 136 RQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAILQQARSAHSVIGTP 195
           RQIL GLLYLHSHDPP+IHRDLKCDNIFVNGNQGEVKIGDLGLAAIL+++ + H V GTP
Sbjct: 135 RQILSGLLYLHSHDPPIIHRDLKCDNIFVNGNQGEVKIGDLGLAAILRKSHAVHCV-GTP 194

Query: 196 EFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYVECANAAQIYKKVSSGIKPASLAKV 255
           EFMAPE+YEEEYNELVDIY+FGMC+LE+VTFEYPY EC +  QIYKKV SG KP +L KV
Sbjct: 195 EFMAPEVYEEEYNELVDIYSFGMCVLEMVTFEYPYSECTHPVQIYKKVISGTKPEALYKV 254

Query: 256 TNPGVKALIEKCIANVSDRLPAKDLLRDPFLLADD--------DHERITRHLKNKTQHTE 315
            +P V+  +EKC+A  S RL A++LL+DPFL  DD        D+  +    +   +H  
Sbjct: 255 KDPMVRQFVEKCLATASRRLSARELLKDPFLQVDDLVFCPGDGDYSLMNYLRQPYLEHAY 314

Query: 316 KREQL-------HIDRCT----------DDTSAETTRDFSMH-------------GQRKD 375
               +        ID  T          DD  A+    F+ H             G++ +
Sbjct: 315 SNVSMMSNGLSESIDEDTPTEDRWDCEDDDIKADGIDLFNGHEDEPLGNVDITIKGRKSE 374

Query: 376 VNKIFLKLRIADALGNVRNIHFPFDIEADTAISVASEMVEELDLSDQDVSTISEMIETEI 405
              IFL+LRIAD  G+VRNI+FPFDIEADTA+SVA+EMV ELD++D +V+ I+EMI+ E+
Sbjct: 375 DGSIFLRLRIADNDGHVRNIYFPFDIEADTALSVATEMVAELDITDHEVTRIAEMIDGEV 434

[Close]

BLAST of MS019989 vs. ExPASy Swiss-Prot
Match: Q9CAV6 (Serine/threonine-protein kinase WNK1 OS=Arabidopsis thaliana OX=3702 GN=WNK1 PE=1 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 4.9e-135
Identity = 262/461 (56.83%), Postives = 310/461 (67.25%), Query Frame = 0


Query: 11  LDESDPEFVEIDPTGRYGRYKEILGKGAYKKVYRAFDELEGIEVAWNQIKVTDVLRNSED 70
           L+    EFVE+DPTGRYGRY E+LGKGA K VYRAFDE EGIEVAWNQ+K+ D L++ ED
Sbjct: 7   LEPDYSEFVEVDPTGRYGRYNEVLGKGASKTVYRAFDEYEGIEVAWNQVKLYDFLQSPED 66

Query: 71  LERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYRKKHKHVDLRA 130
           LERLY E+HLLKTLKHKNI+KFY SWVDT N NINF+TE+FTSGTLRQYR +HK V++RA
Sbjct: 67  LERLYCEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTELFTSGTLRQYRLRHKRVNIRA 126

Query: 131 LKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAILQQARSAHS 190
           +K W RQIL GL YLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAIL+++ +AH 
Sbjct: 127 MKHWCRQILRGLHYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAILRKSHAAHC 186

Query: 191 VIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYVECANAAQIYKKVSSGIKPA 250
           V GTPEFMAPE+YEE YNELVDIY+FGMC+LE+VTF+YPY EC + AQIYKKV SG KP 
Sbjct: 187 V-GTPEFMAPEVYEEAYNELVDIYSFGMCILEMVTFDYPYSECTHPAQIYKKVMSGKKPD 246

Query: 251 SLAKVTNPGVKALIEKCIANVSDRLPAKDLLRDPFLLADD--------DHERITRHLKNK 310
           +L KV +P VK  IEKC+A VS R+ A++LL DPFL  DD        D E     L  +
Sbjct: 247 ALYKVKDPEVKCFIEKCLATVSLRVSARELLDDPFLRIDDGEFDLRSVDMEDSVGPLYRQ 306

Query: 311 TQH------------------------------------------------------TEK 370
             H                                                      TE+
Sbjct: 307 PHHLPDYYNYPSNSSSLNRQYSNGNYPSNSSSLNRQYSNGYNSHHEYQNGWAYNPAETEE 366

Query: 371 RE--QLHIDRCTDDTSAETTR---DFSMHGQRKDVNKIFLKLRIADALGNVRNIHFPFDI 405
               +L   R  DD   E      D ++ G+R+D   +FL+LRIAD  G VRNI+FPFDI
Sbjct: 367 THGIELFESRNNDDQEEEKKSGNVDITIKGKRRDDGGLFLRLRIADKEGRVRNIYFPFDI 426

[Close]

BLAST of MS019989 vs. ExPASy TrEMBL
Match: A0A6J1C5J5 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111008667 PE=4 SV=1)

HSP 1 Score: 1192.6 bits (3084), Expect = 0.0e+00
Identity = 608/609 (99.84%), Postives = 608/609 (99.84%), Query Frame = 0


Query: 1   MSQELSLDQDLDESDPEFVEIDPTGRYGRYKEILGKGAYKKVYRAFDELEGIEVAWNQIK 60
           MSQELSLDQDLDESDPEFVEIDPTGRYGRYKEILGKGAYKKVYRAFDELEGIEVAWNQIK
Sbjct: 1   MSQELSLDQDLDESDPEFVEIDPTGRYGRYKEILGKGAYKKVYRAFDELEGIEVAWNQIK 60

Query: 61  VTDVLRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR 120
           VTDVLRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR
Sbjct: 61  VTDVLRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR 120

Query: 121 KKHKHVDLRALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA 180
           KKHKHVDLRALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA
Sbjct: 121 KKHKHVDLRALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA 180

Query: 181 ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYVECANAAQIY 240
           ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYVECANAAQIY
Sbjct: 181 ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYVECANAAQIY 240

Query: 241 KKVSSGIKPASLAKVTNPGVKALIEKCIANVSDRLPAKDLLRDPFLLADDDHERITRHLK 300
           KKVSSGIKPASLAKVTNPGVKALIEKCIANVSDRLPAKDLLRDPFLLADDDHERITRHLK
Sbjct: 241 KKVSSGIKPASLAKVTNPGVKALIEKCIANVSDRLPAKDLLRDPFLLADDDHERITRHLK 300

Query: 301 NKTQHT-EKREQLHIDRCTDDTSAETTRDFSMHGQRKDVNKIFLKLRIADALGNVRNIHF 360
           NKTQHT EKREQLHIDRCTDDTSAETTRDFSMHGQRKDVNKIFLKLRIADALGNVRNIHF
Sbjct: 301 NKTQHTEEKREQLHIDRCTDDTSAETTRDFSMHGQRKDVNKIFLKLRIADALGNVRNIHF 360

Query: 361 PFDIEADTAISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDWTSVEYGVDNVGGDVA 420
           PFDIEADTAISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDWTSVEYGVDNVGGDVA
Sbjct: 361 PFDIEADTAISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDWTSVEYGVDNVGGDVA 420

Query: 421 ISDSSPSETRNGASPLSIESGNLALEIMPSGRKYWSDSPKGIGGCSPVKPGPSNLSCMDQ 480
           ISDSSPSETRNGASPLSIESGNLALEIMPSGRKYWSDSPKGIGGCSPVKPGPSNLSCMDQ
Sbjct: 421 ISDSSPSETRNGASPLSIESGNLALEIMPSGRKYWSDSPKGIGGCSPVKPGPSNLSCMDQ 480

Query: 481 NVESSGSHMPRDNLDHAAIIKGLESELLSDYGGNGGQDEGSSTTNLHASSDAHPSEENCG 540
           NVESSGSHMPRDNLDHAAIIKGLESELLSDYGGNGGQDEGSSTTNLHASSDAHPSEENCG
Sbjct: 481 NVESSGSHMPRDNLDHAAIIKGLESELLSDYGGNGGQDEGSSTTNLHASSDAHPSEENCG 540

Query: 541 VSERDESEFIEVMAEKLDKLLIQQQKELDELKREHKLVISELLSELTPESYEKVLRICKL 600
           VSERDESEFIEVMAEKLDKLLIQQQKELDELKREHKLVISELLSELTPESYEKVLRICKL
Sbjct: 541 VSERDESEFIEVMAEKLDKLLIQQQKELDELKREHKLVISELLSELTPESYEKVLRICKL 600

Query: 601 EPSDYDSGL 609
           EPSDYDSGL
Sbjct: 601 EPSDYDSGL 609

[Close]

BLAST of MS019989 vs. ExPASy TrEMBL
Match: A0A0A0LU68 (Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_1G046910 PE=4 SV=1)

HSP 1 Score: 998.0 bits (2579), Expect = 1.7e-287
Identity = 510/606 (84.16%), Postives = 555/606 (91.58%), Query Frame = 0


Query: 1   MSQELSLDQDLDESDPEFVEIDPTGRYGRYKEILGKGAYKKVYRAFDELEGIEVAWNQIK 60
           MSQ+LS DQDLDESDPEFVEIDPTGRYGRYKEILGKGA+K+VYRAFDELEGIEVAWNQ+K
Sbjct: 1   MSQDLSPDQDLDESDPEFVEIDPTGRYGRYKEILGKGAFKRVYRAFDELEGIEVAWNQVK 60

Query: 61  VTDVLRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR 120
           VTD+LRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR
Sbjct: 61  VTDLLRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR 120

Query: 121 KKHKHVDLRALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA 180
           KKHKHVD+RALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA
Sbjct: 121 KKHKHVDVRALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA 180

Query: 181 ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYVECANAAQIY 240
           ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPY+ECANAAQIY
Sbjct: 181 ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYIECANAAQIY 240

Query: 241 KKVSSGIKPASLAKVTNPGVKALIEKCIANVSDRLPAKDLLRDPFLLADDDHERITRHLK 300
           KKV+SGIKPASLAKVTN GV+A IEKCIANVSDRLPAKDLLRDPFL ADDDHE I+RHL+
Sbjct: 241 KKVTSGIKPASLAKVTNLGVRAFIEKCIANVSDRLPAKDLLRDPFLQADDDHESISRHLR 300

Query: 301 NKTQHTEKREQLHIDRCTDDTSAETTRDFSMHGQRKDVNKIFLKLRIADALGNVRNIHFP 360
           +KTQ TEK+EQ+  DR  D + AET+RDFSMHG+RKDVNKIFLKLRIAD++GN RNIHFP
Sbjct: 301 SKTQPTEKKEQIDFDRSVDYSPAETSRDFSMHGERKDVNKIFLKLRIADSMGNFRNIHFP 360

Query: 361 FDIEADTAISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDWTSVEYGVDNVGGDVAI 420
           FDIEADTAISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDW SVEY  DNVG D  +
Sbjct: 361 FDIEADTAISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDWISVEYSGDNVGADAPV 420

Query: 421 SDSSPSETRNGASPLSIESGNLALEIMPSGRKYWSDSPKGIGGCSPVKPGPSNLS-CMDQ 480
           SDSSPSETRN ASPLSIESGNLALE+MPSGRKYWSDSPKGIGGCSP+KPGPSNLS   DQ
Sbjct: 421 SDSSPSETRNVASPLSIESGNLALEVMPSGRKYWSDSPKGIGGCSPIKPGPSNLSFASDQ 480

Query: 481 NVESSGSHMPRDNLDHAAIIKGLESELLSDYGGNGGQDEGSSTTNLHASSDAHPSEENCG 540
           NVESS SH+  DNLDHAAIIKGLE+ELLS+ G + GQDE S    +H SS+ H SEEN  
Sbjct: 481 NVESSNSHIHGDNLDHAAIIKGLENELLSEGGDHDGQDESS----IHTSSETHHSEEN-- 540

Query: 541 VSERDESEFIEVMAEKLDKLLIQQQKELDELKREHKLVISELLSELTPESYEKVLRICKL 600
               DES  ++++AEKL+ LL QQQKELDEL+++HKL ISELL++LTPESY+KV+ +C+L
Sbjct: 541 --NYDESVDLKIVAEKLENLLTQQQKELDELRKKHKLDISELLTKLTPESYQKVIEMCQL 598

Query: 601 EPSDYD 606
           +  D++
Sbjct: 601 QHPDFE 598

[Close]

BLAST of MS019989 vs. ExPASy TrEMBL
Match: A0A1S3AY87 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103483855 PE=4 SV=1)

HSP 1 Score: 995.3 bits (2572), Expect = 1.1e-286
Identity = 513/606 (84.65%), Postives = 556/606 (91.75%), Query Frame = 0


Query: 1   MSQELSLDQDLDESDPEFVEIDPTGRYGRYKEILGKGAYKKVYRAFDELEGIEVAWNQIK 60
           MSQ+L LDQDLDESDPEFVEIDPTGRYGRYKEILGKGA+K+VYRAFDELEGIEVAWNQ+K
Sbjct: 1   MSQDLPLDQDLDESDPEFVEIDPTGRYGRYKEILGKGAFKRVYRAFDELEGIEVAWNQVK 60

Query: 61  VTDVLRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR 120
           VTD+LRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR
Sbjct: 61  VTDLLRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR 120

Query: 121 KKHKHVDLRALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA 180
           KKHKHVDLRALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA
Sbjct: 121 KKHKHVDLRALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA 180

Query: 181 ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYVECANAAQIY 240
           ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYVECANAAQIY
Sbjct: 181 ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYVECANAAQIY 240

Query: 241 KKVSSGIKPASLAKVTNPGVKALIEKCIANVSDRLPAKDLLRDPFLLADDDHERITRHLK 300
           KKV+SGIKPASLAKVTN GVK  IEKCIANVSDRL AKDLLRDPFL ADDDH+ I+RHL+
Sbjct: 241 KKVTSGIKPASLAKVTNLGVKTFIEKCIANVSDRLSAKDLLRDPFLQADDDHDSISRHLQ 300

Query: 301 NKTQHTEKREQLHIDRCTDDTSAETTRDFSMHGQRKDVNKIFLKLRIADALGNVRNIHFP 360
           + T+HT KREQ+  DR  D + AET+RDFSMHG+RKDVNKIFLKLRIAD++GN RNIHFP
Sbjct: 301 SSTRHTVKREQIDTDRSIDYSPAETSRDFSMHGERKDVNKIFLKLRIADSMGNFRNIHFP 360

Query: 361 FDIEADTAISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDWTSVEYGVDNVGGDVAI 420
           FDIEADTAISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDW SVEY VDNVG DVAI
Sbjct: 361 FDIEADTAISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDWISVEYSVDNVGADVAI 420

Query: 421 SDSSPSETRNGASPLSIESGNLALEIMPSGRKYWSDSPKGIGGCSPVKPGPSNLS-CMDQ 480
           SDSSPSETRN ASPLSIESGNLALE+MPSGRKYWSDSPKGIGGCSP+KPGPSNLS   D 
Sbjct: 421 SDSSPSETRNVASPLSIESGNLALEVMPSGRKYWSDSPKGIGGCSPIKPGPSNLSFTTDL 480

Query: 481 NVESSGSHMPRDNLDHAAIIKGLESELLSDYGGNGGQDEGSSTTNLHASSDAHPSEENCG 540
           NVESS SH+  DNLDHAAIIKGLE+ELLS+ G + GQD GSS   +H SS+ H SEEN  
Sbjct: 481 NVESSNSHVHGDNLDHAAIIKGLENELLSEGGDHDGQD-GSS---IHTSSETHYSEEN-- 540

Query: 541 VSERDESEFIEVMAEKLDKLLIQQQKELDELKREHKLVISELLSELTPESYEKVLRICKL 600
               DES  ++++AEKL+ LL QQQKELDEL+++HKL ISELL++LTPESY+KV+++C+L
Sbjct: 541 --NYDESVDLKIVAEKLENLLTQQQKELDELRKKHKLAISELLTKLTPESYQKVIKMCQL 598

Query: 601 EPSDYD 606
           +  D++
Sbjct: 601 KRPDFE 598

[Close]

BLAST of MS019989 vs. ExPASy TrEMBL
Match: A0A6J1GR70 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111456759 PE=4 SV=1)

HSP 1 Score: 991.1 bits (2561), Expect = 2.0e-285
Identity = 509/604 (84.27%), Postives = 550/604 (91.06%), Query Frame = 0


Query: 1   MSQELSLDQDLDESDPEFVEIDPTGRYGRYKEILGKGAYKKVYRAFDELEGIEVAWNQIK 60
           MSQ+LSLDQD DESDPEFVEIDPTGRYGRYKEILGKGA+KKVYRAFDELEGIEVAWNQ+K
Sbjct: 1   MSQDLSLDQDPDESDPEFVEIDPTGRYGRYKEILGKGAFKKVYRAFDELEGIEVAWNQVK 60

Query: 61  VTDVLRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR 120
           VTD+LRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR
Sbjct: 61  VTDLLRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR 120

Query: 121 KKHKHVDLRALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA 180
           KKHKHVDLRALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA
Sbjct: 121 KKHKHVDLRALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA 180

Query: 181 ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYVECANAAQIY 240
           ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPY+ECANAAQIY
Sbjct: 181 ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYIECANAAQIY 240

Query: 241 KKVSSGIKPASLAKVTNPGVKALIEKCIANVSDRLPAKDLLRDPFLLADDDHERITRHLK 300
           KKV+SGIKPASLAKVT+ GV+A IEKCIANVSDRLPAKDLLRDPFLLAD++HE I+RHL 
Sbjct: 241 KKVTSGIKPASLAKVTDGGVRAFIEKCIANVSDRLPAKDLLRDPFLLADEEHESISRHLH 300

Query: 301 NKTQHTEKREQLHIDRCTDDTSAETTRDFSMHGQRKDVNKIFLKLRIADALGNVRNIHFP 360
            K  H EKREQ+  D   DDT A T+ DFSMHGQRKDVNKIFLKLRIAD++GN RNIHFP
Sbjct: 301 PKIHHKEKREQIDTDTSVDDTPA-TSVDFSMHGQRKDVNKIFLKLRIADSMGNFRNIHFP 360

Query: 361 FDIEADTAISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDWTSVEYGVDNVGGDVAI 420
           FDIEADT+ISVASEMVEELDLSDQDVSTISEMIE+EIRSYIPDWTSVEY VDNVG DV I
Sbjct: 361 FDIEADTSISVASEMVEELDLSDQDVSTISEMIESEIRSYIPDWTSVEYPVDNVGADVVI 420

Query: 421 SDSSPSETRNGASPLSIESGNLALEIMPSGRKYWSDSPKGIGGCSPVKPGPSNLS-CMDQ 480
           SDSSPS++R  ASPLSIESGNLALE++PSGRKYWSDSPKG+GGCSPVKPGPSNLS   +Q
Sbjct: 421 SDSSPSDSRTAASPLSIESGNLALEVLPSGRKYWSDSPKGVGGCSPVKPGPSNLSFASEQ 480

Query: 481 NVESSGSHMPRDNLDHAAIIKGLESELLSDYGGNGGQDEGSSTTNLHASSDAHPSEENCG 540
           NVESS SHM  DN+DHAAII GLE+ELLS+   N GQDE   TT++HASS+AHP      
Sbjct: 481 NVESSSSHMHEDNIDHAAIIMGLENELLSEDAANDGQDESILTTDIHASSEAHP------ 540

Query: 541 VSERDESEFIEVMAEKLDKLLIQQQKELDELKREHKLVISELLSELTPESYEKVLRICKL 600
            S+ DES  IE+M EKL+ LL QQ++ELDELK + KL ISELL+ELTPE+++KVL+ICKL
Sbjct: 541 -SDYDESIGIEIMTEKLENLLTQQKEELDELKTKQKLAISELLAELTPETHQKVLKICKL 596

Query: 601 EPSD 604
           + SD
Sbjct: 601 KHSD 596

[Close]

BLAST of MS019989 vs. ExPASy TrEMBL
Match: A0A6J1K5K4 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111490260 PE=4 SV=1)

HSP 1 Score: 987.3 bits (2551), Expect = 2.9e-284
Identity = 509/604 (84.27%), Postives = 549/604 (90.89%), Query Frame = 0


Query: 1   MSQELSLDQDLDESDPEFVEIDPTGRYGRYKEILGKGAYKKVYRAFDELEGIEVAWNQIK 60
           MSQ+LSLDQD DESDPEFVEIDPTGRYGRYKEILGKGA+KKVYRAFDELEGIEVAWNQ+K
Sbjct: 1   MSQDLSLDQDPDESDPEFVEIDPTGRYGRYKEILGKGAFKKVYRAFDELEGIEVAWNQVK 60

Query: 61  VTDVLRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR 120
           VTD+LRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR
Sbjct: 61  VTDLLRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYR 120

Query: 121 KKHKHVDLRALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA 180
           KKHKHVDLRALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA
Sbjct: 121 KKHKHVDLRALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAA 180

Query: 181 ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYVECANAAQIY 240
           ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPY+ECANAAQIY
Sbjct: 181 ILQQARSAHSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYIECANAAQIY 240

Query: 241 KKVSSGIKPASLAKVTNPGVKALIEKCIANVSDRLPAKDLLRDPFLLADDDHERITRHLK 300
           KKV+SGIKPASLAKVT+ GV+A IEKCIANVSDRLPAKDLLRDPFLLAD+DHE I+RHL 
Sbjct: 241 KKVTSGIKPASLAKVTDGGVRAFIEKCIANVSDRLPAKDLLRDPFLLADEDHESISRHLH 300

Query: 301 NKTQHTEKREQLHIDRCTDDTSAETTRDFSMHGQRKDVNKIFLKLRIADALGNVRNIHFP 360
            K  H EKRE++  DR  DDT A T+ DFSMHGQRKDVNKIFLKLRIAD++GN RNIHFP
Sbjct: 301 PKIHHKEKREKIDTDRSVDDTPA-TSVDFSMHGQRKDVNKIFLKLRIADSMGNFRNIHFP 360

Query: 361 FDIEADTAISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDWTSVEYGVDNVGGDVAI 420
           FDIEADT+ISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDWT VEY VDNVG +V I
Sbjct: 361 FDIEADTSISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDWTLVEYPVDNVGAEVVI 420

Query: 421 SDSSPSETRNGASPLSIESGNLALEIMPSGRKYWSDSPKGIGGCSPVKPGPSNLS-CMDQ 480
           SDSSPS++R  ASPLSIESGNLALE++PSGRKYWSDSPKG+GGCSPVKPGPSNLS   +Q
Sbjct: 421 SDSSPSDSRTAASPLSIESGNLALEVLPSGRKYWSDSPKGMGGCSPVKPGPSNLSFASEQ 480

Query: 481 NVESSGSHMPRDNLDHAAIIKGLESELLSDYGGNGGQDEGSSTTNLHASSDAHPSEENCG 540
           NVESS SHM  DN+DHAAII GLE+ELLS+ G N GQDE   TT++HASS+AHP      
Sbjct: 481 NVESSSSHMHEDNIDHAAIIMGLENELLSEDGANDGQDESILTTDIHASSEAHP------ 540

Query: 541 VSERDESEFIEVMAEKLDKLLIQQQKELDELKREHKLVISELLSELTPESYEKVLRICKL 600
            S+ DES  IE+M EKL+ LL QQ++EL ELK + KL ISELL+ELTPE+++KVL ICKL
Sbjct: 541 -SDYDESIGIEIMTEKLELLLTQQKEELVELKTKQKLAISELLAELTPETHQKVLAICKL 596

Query: 601 EPSD 604
           + SD
Sbjct: 601 KHSD 596

[Close]

BLAST of MS019989 vs. TAIR 10
Match: AT3G48260.1 (with no lysine (K) kinase 3 )

HSP 1 Score: 583.6 bits (1503), Expect = 1.9e-166
Identity = 333/599 (55.59%), Postives = 403/599 (67.28%), Query Frame = 0


Query: 9   QDLDESDPEFVEIDPTGRYGRYKEILGKGAYKKVYRAFDELEGIEVAWNQIKVTDVLRNS 68
           QD + S+ EFVEIDPTGRYGRYKE+LGKGA+K+VYRAFD+LEGIEVAWNQ+K+ D   +S
Sbjct: 3   QDENNSEEEFVEIDPTGRYGRYKEVLGKGAFKEVYRAFDQLEGIEVAWNQVKLDDKFCSS 62

Query: 69  EDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYRKKHKHVDL 128
           EDL+RLYSEVHLLKTLKHK+IIKFY SW+D ++  IN ITE+FTSG LRQYRKKHK VDL
Sbjct: 63  EDLDRLYSEVHLLKTLKHKSIIKFYTSWIDHQHMTINLITEVFTSGNLRQYRKKHKCVDL 122

Query: 129 RALKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAILQQARSA 188
           RALKKWSRQILEGL+YLHSHDPPVIHRDLKCDNIF+NGNQGEVKIGDLGLAAIL +ARSA
Sbjct: 123 RALKKWSRQILEGLVYLHSHDPPVIHRDLKCDNIFINGNQGEVKIGDLGLAAILHRARSA 182

Query: 189 HSVIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYVECANAAQIYKKVSSGIK 248
           HSVIGTPEFMAPELYEE+YN LVDIYAFGMCLLELVTFEYPY EC NAAQIY+KV+SGIK
Sbjct: 183 HSVIGTPEFMAPELYEEDYNVLVDIYAFGMCLLELVTFEYPYSECTNAAQIYRKVTSGIK 242

Query: 249 PASLAKVTNPGVKALIEKCIANVSDRLPAKDLLRDPFLLA-DDDHERITRHLKNKTQHTE 308
           PA+L  VT+P V+A IEKCIA VS RL AK+LL DPFL    ++ E ++ H +N      
Sbjct: 243 PAALLNVTDPQVRAFIEKCIAKVSQRLSAKELLDDPFLKCYKENTENVSSHKENGYNGNG 302

Query: 309 KREQLHIDRCTDDTSAETTRDFSMHGQRKDVNKIFLKLRIADALGNVRNIHFPFDIEADT 368
                 +D+ +D      T    + GQRKD+N IFLKLRI D+ G +RNIHFPF+IE DT
Sbjct: 303 -----IVDKLSDSEVGLLT----VEGQRKDLNTIFLKLRITDSKGQIRNIHFPFNIETDT 362

Query: 369 AISVASEMVEELDLS-DQDVSTISEMIETEIRSYIPDWTSVEYGVDNVGGDVAISD--SS 428
           + SVA EMVEELDL+ DQD+STI++MI+TEI S+IPDWT        +G D A+    SS
Sbjct: 363 SFSVAIEMVEELDLTDDQDISTIAKMIDTEIHSHIPDWTPSRL----IGDDSAVQKCLSS 422

Query: 429 PSETRNGASPLSIESGNLALEIMPSGRKYWSDSPKGIGGCSPVKPGPSNLSCMDQNVESS 488
           P                L L+  PSGRK+WS    G G                   +S 
Sbjct: 423 PE--------------TLHLDRFPSGRKFWSSPKAGAG-------------------DSR 482

Query: 489 GSHMPRDNLDHAAIIKGLESELLSDYGGNGGQDEGSSTTNLHASSDAHPSEENCGVSERD 548
               PR N           S+L S  G                     P  +  G     
Sbjct: 483 SPFAPRSN-----------SKLSSAQG---------------------PINQEVG----- 512

Query: 549 ESEFIEVMAEKLDKLLIQQQKELDELKREHKLVISELLSELTPESYEKVLRICKLEPSD 604
                 V+ EKL+ LL +Q++E++E++R+ + +++E L E  PE  E+ L   +++ SD
Sbjct: 543 ------VIVEKLESLLRKQREEIEEMQRDQERIVTEFLKEFPPEICEEALVRLQVKDSD 512

[Close]

BLAST of MS019989 vs. TAIR 10
Match: AT3G04910.1 (with no lysine (K) kinase 1 )

HSP 1 Score: 483.0 bits (1242), Expect = 3.5e-136
Identity = 262/461 (56.83%), Postives = 310/461 (67.25%), Query Frame = 0


Query: 11  LDESDPEFVEIDPTGRYGRYKEILGKGAYKKVYRAFDELEGIEVAWNQIKVTDVLRNSED 70
           L+    EFVE+DPTGRYGRY E+LGKGA K VYRAFDE EGIEVAWNQ+K+ D L++ ED
Sbjct: 7   LEPDYSEFVEVDPTGRYGRYNEVLGKGASKTVYRAFDEYEGIEVAWNQVKLYDFLQSPED 66

Query: 71  LERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYRKKHKHVDLRA 130
           LERLY E+HLLKTLKHKNI+KFY SWVDT N NINF+TE+FTSGTLRQYR +HK V++RA
Sbjct: 67  LERLYCEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTELFTSGTLRQYRLRHKRVNIRA 126

Query: 131 LKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAILQQARSAHS 190
           +K W RQIL GL YLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAIL+++ +AH 
Sbjct: 127 MKHWCRQILRGLHYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAILRKSHAAHC 186

Query: 191 VIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYVECANAAQIYKKVSSGIKPA 250
           V GTPEFMAPE+YEE YNELVDIY+FGMC+LE+VTF+YPY EC + AQIYKKV SG KP 
Sbjct: 187 V-GTPEFMAPEVYEEAYNELVDIYSFGMCILEMVTFDYPYSECTHPAQIYKKVMSGKKPD 246

Query: 251 SLAKVTNPGVKALIEKCIANVSDRLPAKDLLRDPFLLADD--------DHERITRHLKNK 310
           +L KV +P VK  IEKC+A VS R+ A++LL DPFL  DD        D E     L  +
Sbjct: 247 ALYKVKDPEVKCFIEKCLATVSLRVSARELLDDPFLRIDDGEFDLRSVDMEDSVGPLYRQ 306

Query: 311 TQH------------------------------------------------------TEK 370
             H                                                      TE+
Sbjct: 307 PHHLPDYYNYPSNSSSLNRQYSNGNYPSNSSSLNRQYSNGYNSHHEYQNGWAYNPAETEE 366

Query: 371 RE--QLHIDRCTDDTSAETTR---DFSMHGQRKDVNKIFLKLRIADALGNVRNIHFPFDI 405
               +L   R  DD   E      D ++ G+R+D   +FL+LRIAD  G VRNI+FPFDI
Sbjct: 367 THGIELFESRNNDDQEEEKKSGNVDITIKGKRRDDGGLFLRLRIADKEGRVRNIYFPFDI 426

[Close]

BLAST of MS019989 vs. TAIR 10
Match: AT5G28080.2 (Protein kinase superfamily protein )

HSP 1 Score: 469.2 bits (1206), Expect = 5.2e-132
Identity = 248/435 (57.01%), Postives = 306/435 (70.34%), Query Frame = 0


Query: 11  LDESDPEFVEIDPTGRYGRYKEILGKGAYKKVYRAFDELEGIEVAWNQIKVTDVLRNSED 70
           L+    E+VE+DPTGRYGRY E+LGKG+ K VYR FDE +GIEVAWNQ+K+ D L++ ++
Sbjct: 8   LESDYSEYVEVDPTGRYGRYNEVLGKGSSKTVYRGFDEYQGIEVAWNQVKLYDFLQSPQE 67

Query: 71  LERLYSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYRKKHKHVDLRA 130
           LERLY E+HLLKTLKHK+I+KFY SWVDT N NINF+TE+FTSGTLRQYR KHK V++RA
Sbjct: 68  LERLYCEIHLLKTLKHKSIMKFYASWVDTDNRNINFVTEMFTSGTLRQYRLKHKRVNIRA 127

Query: 131 LKKWSRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAILQQARSAHS 190
           +K W RQIL GL YLH+HDPPVIHRDLKCDNIF+NGNQGEVKIGDLGLAA LQ + +AH 
Sbjct: 128 VKNWCRQILRGLNYLHTHDPPVIHRDLKCDNIFINGNQGEVKIGDLGLAACLQHSHAAHC 187

Query: 191 VIGTPEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYVECANAAQIYKKVSSGIKPA 250
           V GTPEFMAPE+Y+EEYN+LVDIY+FGMC+LE+VTF+YPY EC++ AQIYK+V SG KP 
Sbjct: 188 V-GTPEFMAPEVYKEEYNQLVDIYSFGMCVLEMVTFDYPYSECSHPAQIYKRVISGKKPD 247

Query: 251 SLAKVTNPGVKALIEKCIANVSDRLPAKDLLRDPFLLADDDHERITRHLKNKTQH--TEK 310
            L KV +P V+  IEKC+A VS RL A +LL D FL  D+   R     K       T  
Sbjct: 248 GLDKVKDPEVRGFIEKCLATVSLRLSACELLDDHFLCIDESDMRRVESEKGLIDEAGTPL 307

Query: 311 REQLHIDRCT---------------------------------DDTSAETTR----DFSM 370
           R   HI   +                                 DD   E  R    D S+
Sbjct: 308 RHSYHIPHYSNGYYSLYNQNQWDYNGDETVESHEIDLLEFQNDDDEEEEDKRFGSVDISI 367

Query: 371 HGQRKD-VNKIFLKLRIADALGNVRNIHFPFDIEADTAISVASEMVEELDLSDQDVSTIS 406
            G+R+D  + +FL+L+  +  G VRNI+FPFDIE DTAISVA EMVEEL++ D+DV+ I+
Sbjct: 368 KGKRRDNGDGLFLRLKTVNKEGCVRNIYFPFDIETDTAISVAREMVEELEMDDRDVTKIA 427

[Close]

BLAST of MS019989 vs. TAIR 10
Match: AT3G22420.1 (with no lysine (K) kinase 2 )

HSP 1 Score: 468.0 bits (1203), Expect = 1.2e-131
Identity = 244/422 (57.82%), Postives = 309/422 (73.22%), Query Frame = 0


Query: 18  FVEIDPTGRYGRYKEILGKGAYKKVYRAFDELEGIEVAWNQIKVTDVLRNSEDLERLYSE 77
           FVEIDP+GRYGRY EILGKGA K VYRAFDE EGIEVAWNQ+K+ +  RN E+LE+ + E
Sbjct: 14  FVEIDPSGRYGRYDEILGKGASKTVYRAFDEYEGIEVAWNQVKLRNFTRNPEELEKFFRE 73

Query: 78  VHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYRKKHKHVDLRALKKWSRQ 137
           +HLLKTL H+NI+KFY SWVDT N +INF+TE+FTSGTLRQYR +H+ V++RA+K+W +Q
Sbjct: 74  IHLLKTLNHQNIMKFYTSWVDTNNLSINFVTELFTSGTLRQYRLRHRRVNIRAVKQWCKQ 133

Query: 138 ILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAILQQARSAHSV--IGTP 197
           IL+GLLYLHS  PP+IHRDLKCDNIF+NGNQGEVKIGDLGLAAIL   R +H+V  +GTP
Sbjct: 134 ILKGLLYLHSRSPPIIHRDLKCDNIFINGNQGEVKIGDLGLAAIL---RKSHAVRCVGTP 193

Query: 198 EFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYVECANAAQIYKKVSSGIKPASLAKV 257
           EFMAPE+Y+EEYNELVD+YAFGMC+LE+VTF+YPY EC + AQIYKKV+SG KP +   V
Sbjct: 194 EFMAPEVYDEEYNELVDVYAFGMCVLEMVTFDYPYSECTHPAQIYKKVTSGKKPEAFYLV 253

Query: 258 TNPGVKALIEKCIANVSDRLPAKDLLRDPFLLAD----------------DDHERITRH- 317
            +P V+  +EKC+ANV+ RL A +LL+DPFL  D                D+     RH 
Sbjct: 254 KDPEVREFVEKCLANVTCRLTALELLQDPFLQDDNMDGFVMRPIDYYNGYDETGVFLRHP 313

Query: 318 -LKNKTQHT--EKREQLHIDRCTDDTSAETTRDFSMHGQRKDVNKIFLKLRIADALGNVR 377
            + +   H   E  +   ID   +D   E   D S+ G+R   + IFL+LRI+DA G +R
Sbjct: 314 LIDDPLYHDQFESSQICEIDLFANDD--EDHVDISIKGKRNGDDGIFLRLRISDAEGRIR 373

Query: 378 NIHFPFDIEADTAISVASEMVEELDLSDQDVSTISEMIETEIRSYIPDWTSVEYGVDNVG 418
           NI+FPF+   DTA SVA EMV ELD+++QDV+ I+EMI+ EI + +PDW +      NV 
Sbjct: 374 NIYFPFETAIDTAWSVAVEMVSELDITNQDVAKIAEMIDAEIAALVPDWKNDTESSQNVN 430

[Close]

BLAST of MS019989 vs. TAIR 10
Match: AT3G18750.1 (with no lysine (K) kinase 6 )

HSP 1 Score: 456.1 bits (1172), Expect = 4.5e-128
Identity = 237/425 (55.76%), Postives = 302/425 (71.06%), Query Frame = 0


Query: 15  DPEFVEIDPTGRYGRYKEILGKGAYKKVYRAFDELEGIEVAWNQIKVTDVLRNSEDLERL 74
           DPE +E+DPT RY RYKE++GKGA+K VY+AFDE++GIEVAWNQ+++ DVL++   LERL
Sbjct: 15  DPEVLEVDPTFRYIRYKEVIGKGAFKTVYKAFDEVDGIEVAWNQVRIDDVLQSPNCLERL 74

Query: 75  YSEVHLLKTLKHKNIIKFYNSWVDTKNENINFITEIFTSGTLRQYRKKHKHVDLRALKKW 134
           YSEV LLK+LKH NII+FYNSW+D KN+ +N ITE+FTSG+LR YRKKH+ V+++A+K W
Sbjct: 75  YSEVRLLKSLKHNNIIRFYNSWIDDKNKTVNIITELFTSGSLRHYRKKHRKVNMKAVKNW 134

Query: 135 SRQILEGLLYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAILQQARSAHSVIGT 194
           +RQIL GL YLH  +PP+IHRDLKCDNIF+NGN GEVKIGDLGLA +++QA +A SVIGT
Sbjct: 135 ARQILMGLRYLHGQEPPIIHRDLKCDNIFINGNHGEVKIGDLGLATVMEQA-NAKSVIGT 194

Query: 195 PEFMAPELYEEEYNELVDIYAFGMCLLELVTFEYPYVECANAAQIYKKVSSGIKPASLAK 254
           PEFMAPELY+E YNEL DIY+FGMC+LE+VTF+YPY EC N+AQIYKKVSSGIKPASL++
Sbjct: 195 PEFMAPELYDENYNELADIYSFGMCMLEMVTFDYPYCECKNSAQIYKKVSSGIKPASLSR 254

Query: 255 VTNPGVKALIEKCIANVSDRLPAKDLLRDPFL------------LAD------------- 314
           V +P VK  IEKC+   S+RL AK+LL DPFL            L D             
Sbjct: 255 VKDPEVKQFIEKCLLPASERLSAKELLLDPFLQLNGLTMNNPLPLPDIVMPKEGAFGDRC 314

Query: 315 --DDHERITRHLKNKTQHTEKREQLHI--------DRCTDDTSAETTRDFSMHGQRKDVN 374
              +    TR  K  +   ++   L I         RC +   A+    F + G+  D  
Sbjct: 315 LMSEGPPTTRPSKTLSIDLDEDSNLPIVTFSDNSGSRCIEVRRAKRGNFFVLKGEENDEQ 374

Query: 375 KIFLKLRIADALGNVRNIHFPFDIEADTAISVASEMVEELDLSDQDVSTISEMIETEIRS 405
            + L LRI D  G VRNIHF F  E DTA  V+SEMVE+L+L+DQ+V+ I+E+I+  + +
Sbjct: 375 SVSLILRIVDENGRVRNIHFLFYQEGDTASKVSSEMVEQLELTDQNVTFIAELIDILLVN 434

The following BLAST results are available for this feature:

BLAST of MS019989 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022137111.1	0.0e+00	99.84	probable serine/threonine-protein kinase WNK3 [Momordica charantia]	[more]
XP_038894587.1	6.7e-291	85.31	probable serine/threonine-protein kinase WNK3 [Benincasa hispida]	[more]
XP_011651790.1	3.4e-287	84.16	probable serine/threonine-protein kinase WNK3 [Cucumis sativus] >XP_031740994.1 ...	[more]
XP_008438930.1	2.2e-286	84.65	PREDICTED: probable serine/threonine-protein kinase WNK3 [Cucumis melo]	[more]
XP_023542613.1	1.4e-285	84.27	probable serine/threonine-protein kinase WNK3 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS019989 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q65X23	3.6e-170	55.00	Probable serine/threonine-protein kinase WNK2 OS=Oryza sativa subsp. japonica OX...	[more]
Q9STK6	2.6e-165	55.59	Probable serine/threonine-protein kinase WNK3 OS=Arabidopsis thaliana OX=3702 GN...	[more]
A2YMV6	7.5e-136	58.55	Probable serine/threonine-protein kinase WNK1 OS=Oryza sativa subsp. indica OX=3...	[more]
Q0D598	7.5e-136	58.55	Probable serine/threonine-protein kinase WNK1 OS=Oryza sativa subsp. japonica OX...	[more]
Q9CAV6	4.9e-135	56.83	Serine/threonine-protein kinase WNK1 OS=Arabidopsis thaliana OX=3702 GN=WNK1 PE=...	[more]

BLAST of MS019989 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C5J5	0.0e+00	99.84	Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=L...	[more]
A0A0A0LU68	1.7e-287	84.16	Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_1...	[more]
A0A1S3AY87	1.1e-286	84.65	Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10348...	[more]
A0A6J1GR70	2.0e-285	84.27	Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1K5K4	2.9e-284	84.27	Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]

BLAST of MS019989 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G48260.1	1.9e-166	55.59	with no lysine (K) kinase 3	[more]
AT3G04910.1	3.5e-136	56.83	with no lysine (K) kinase 1	[more]
AT5G28080.2	5.2e-132	57.01	Protein kinase superfamily protein	[more]
AT3G22420.1	1.2e-131	57.82	with no lysine (K) kinase 2	[more]
AT3G18750.1	4.5e-128	55.76	with no lysine (K) kinase 6	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 553..580
None	No IPR available	GENE3D	3.10.20.90		coord: 339..409 e-value: 1.9E-6 score: 30.1
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 12..109 e-value: 3.4E-35 score: 122.0
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 111..293 e-value: 5.3E-48 score: 164.9
None	No IPR available	PIRSR	PIRSR630220-1	PIRSR630220-1	coord: 32..289 e-value: 4.5E-18 score: 62.6
None	No IPR available	PIRSR	PIRSR038172-1	PIRSR038172-1	coord: 18..253 e-value: 1.6E-28 score: 97.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 514..535
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 508..540
None	No IPR available	PANTHER	PTHR13902:SF153	SERINE/THREONINE-PROTEIN KINASE-RELATED	coord: 8..602
None	No IPR available	PANTHER	PTHR13902	SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATED	coord: 8..602
None	No IPR available	CDD	cd13983	STKc_WNK	coord: 26..286 e-value: 1.84567E-163 score: 465.162
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 28..286 e-value: 1.3E-59 score: 214.1
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 31..286 e-value: 1.2E-50 score: 172.3
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 28..286 score: 40.555405
IPR024678	Serine/threonine-protein kinase OSR1/WNK, CCT domain	PFAM	PF12202	OSR1_C	coord: 343..397 e-value: 6.9E-6 score: 26.0
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 152..164
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 30..287

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS019989.1	MS019989.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity
molecular_function	GO:0004674	protein serine/threonine kinase activity