MS019981 (gene) Bitter gourd (TR) v1

Overview
NameMS019981
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionPMEI domain-containing protein
Locationscaffold22: 940849 .. 941356 (+)
RNA-Seq ExpressionMS019981
SyntenyMS019981
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTAGATCAGACCAACACAACCATCAATAATTTTGTCAATGATACTTGCAACAAGCTTGTTCAGTATGACCAACTATATAAGTTGTGTATTCCCGAAATAGAGTCGGATCCTCGTGAAGATTTGAAGAAAAAATTTGACTGGTCTTCTCATTTTATTTGTAAATCATAGCATCAGCGATTTCAACGCCACCATTTCGTTCCTTCACAAAGAGATCATTTCGGGCAAGCTAGATCGTGATACAAAAGACATGCACGAATCACGTTCAGAAGACTATAATATGGGGCTTGATGATCTTCATGAAACTGTACATAGATTACTAACCAAACAAAGTACGGACGTGCAGAAATTGGTGAATCACGCTGATACCGTTATAGGTGGATGTGAGGATGATTTTGATGCATATCCATGGTCTCTCGAGTGGAAATCTCGTTACAAAACCTCCTACGCATTATTGAATCTCTCACTTGCAATGATAAATCTCATTAAATGTAATCATGTAGAAGGATGT

mRNA sequence

GTAGATCAGACCAACACAACCATCAATAATTTTGTCAATGATACTTGCAACAAGCTTGTTCAGTATGACCAACTATATAAGTTGTGTATTCCCGAAATAGAGTCGGATCCTCGTGAAGATTTGAAGAATTTGACTGGTCTTCTCATTTTATTTGTAAATCATAGCATCAGCGATTTCAACGCCACCATTTCGTTCCTTCACAAAGAGATCATTTCGGGCAAGCTAGATCGTGATACAAAAGACATGCACGAATCACGTTCAGAAGACTATAATATGGGGCTTGATGATCTTCATGAAACTGTACATAGATTACTAACCAAACAAAGTACGGACGTGCAGAAATTGGTGAATCACGCTGATACCGTTATAGGTGGATGTGAGGATGATTTTGATGCATATCCATGGTCTCTCGAGTGGAAATCTCGTTACAAAACCTCCTACGCATTATTGAATCTCTCACTTGCAATGATAAATCTCATTAAATGTAATCATGTAGAAGGATGT

Coding sequence (CDS)

GTAGATCAGACCAACACAACCATCAATAATTTTGTCAATGATACTTGCAACAAGCTTGTTCAGTATGACCAACTATATAAGTTGTGTATTCCCGAAATAGAGTCGGATCCTCGTGAAGATTTGAAGAATTTGACTGGTCTTCTCATTTTATTTGTAAATCATAGCATCAGCGATTTCAACGCCACCATTTCGTTCCTTCACAAAGAGATCATTTCGGGCAAGCTAGATCGTGATACAAAAGACATGCACGAATCACGTTCAGAAGACTATAATATGGGGCTTGATGATCTTCATGAAACTGTACATAGATTACTAACCAAACAAAGTACGGACGTGCAGAAATTGGTGAATCACGCTGATACCGTTATAGGTGGATGTGAGGATGATTTTGATGCATATCCATGGTCTCTCGAGTGGAAATCTCGTTACAAAACCTCCTACGCATTATTGAATCTCTCACTTGCAATGATAAATCTCATTAAATGTAATCATGTAGAAGGATGT

Protein sequence

VDQTNTTINNFVNDTCNKLVQYDQLYKLCIPEIESDPREDLKNLTGLLILFVNHSISDFNATISFLHKEIISGKLDRDTKDMHESRSEDYNMGLDDLHETVHRLLTKQSTDVQKLVNHADTVIGGCEDDFDAYPWSLEWKSRYKTSYALLNLSLAMINLIKCNHVEGC
Homology
BLAST of MS019981 vs. NCBI nr
Match: XP_023511468.1 (uncharacterized protein LOC111776286 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 162.5 bits (410), Expect = 3.1e-36
Identity = 87/169 (51.48%), Postives = 115/169 (68.05%), Query Frame = 0

Query: 2   DQTNTTINNFVNDTCNKLVQYDQLYKLCIPEIESDPREDLK-NLTGLLILFVNHSISDFN 61
           ++  +T + FV+DTCN+  QY++LYKLCIPEI+ DPREDLK NLTG L +FVNH+I++FN
Sbjct: 3   EEDKSTDDTFVHDTCNRF-QYNELYKLCIPEIDLDPREDLKSNLTGFLTIFVNHTINNFN 62

Query: 62  ATISFLHKEIISGKLDRDTKDMHESRSEDYNMGLDDLHETVHRLLTKQSTDVQKL-VNHA 121
             + FL +EI SGKLD +TK M++  SE + MG   L E++H LLT+  T+V  L     
Sbjct: 63  DNLIFLQREIKSGKLDAETKLMYDKCSESFGMGYTKLQESMHILLTQPGTNVYDLPTMSV 122

Query: 122 DTVIGGCEDDFDAYPWSLEWKSRYKTSYALLNLSLAMINLIKCNHVEGC 169
           D  I  C DDF+  P   EW+SRYKTS+ L++L L   NLIKCN    C
Sbjct: 123 DKRISVCFDDFEDDPIPPEWQSRYKTSWNLVSLILGTSNLIKCNREASC 170

BLAST of MS019981 vs. NCBI nr
Match: KAG6572151.1 (putative pectinesterase/pectinesterase inhibitor 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 158.3 bits (399), Expect = 5.8e-35
Identity = 88/170 (51.76%), Postives = 112/170 (65.88%), Query Frame = 0

Query: 1   VDQTNTTINNFVNDTCNKLVQYDQLYKLCIPEIESDPREDLK-NLTGLLILFVNHSISDF 60
           V++T +T   FV+DTCNK  Q +QLYKLCIPEI+SDPREDLK NLTG+LI+FVNHS S+F
Sbjct: 332 VEETRST---FVHDTCNKF-QIEQLYKLCIPEIDSDPREDLKSNLTGILIIFVNHSFSNF 391

Query: 61  NATISFLHKEIISGKLDRDTKDMHESRSEDYNMGLDDLHETVHRLLTKQSTDVQKLVN-H 120
           N  I+FL +EI SGKL+ +TK M+    ED+  G  DL ET+H LLT+   D   L   +
Sbjct: 392 NDNIAFLQREIKSGKLNGETKVMYNGCLEDFERGNGDLQETMHILLTQTGIDAYNLPTIN 451

Query: 121 ADTVIGGCEDDFDAYPWSLEWKSRYKTSYALLNLSLAMINLIKCNHVEGC 169
               +  C D ++  P   EW+SRY TS+ LL L     NL+KCNH   C
Sbjct: 452 IPNYVSECVDFYEGDPIPPEWQSRYNTSWNLLTLIGVTSNLMKCNHDIAC 497

BLAST of MS019981 vs. NCBI nr
Match: KAG6572152.1 (Expansin-like A2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 157.9 bits (398), Expect = 7.6e-35
Identity = 81/165 (49.09%), Postives = 112/165 (67.88%), Query Frame = 0

Query: 6   TTINNFVNDTCNKLVQYDQLYKLCIPEIESDPREDLK-NLTGLLILFVNHSISDFNATIS 65
           +T NNF +DTCN+  Q++QLYKLCIPE++S+PREDLK NLTG+LI+FVNH+IS+F   + 
Sbjct: 150 STNNNFAHDTCNRF-QFEQLYKLCIPEVDSEPREDLKSNLTGILIIFVNHTISNFKDNVM 209

Query: 66  FLHKEIISGKLDRDTKDMHESRSEDYNMGLDDLHETVHRLLTKQSTDVQKL-VNHADTVI 125
           FL +EI SGKL+  TK+M+++  E++  G  DL +++H LLT+   D+  L      + +
Sbjct: 210 FLQREIKSGKLNGKTKEMYDTCLENFERGTTDLQQSMHVLLTETGKDIYGLPALSVASYV 269

Query: 126 GGCEDDFDAYPWSLEWKSRYKTSYALLNLSLAMINLIKCNHVEGC 169
             C DDF+  P   EW+SRY TS  LL L L   NL+KCN    C
Sbjct: 270 TECVDDFEGVPKPPEWQSRYNTSNKLLELILGTCNLMKCNRELAC 313

BLAST of MS019981 vs. NCBI nr
Match: KAG6572380.1 (ATP-dependent zinc metalloprotease FTSH 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 155.6 bits (392), Expect = 3.8e-34
Identity = 86/157 (54.78%), Postives = 110/157 (70.06%), Query Frame = 0

Query: 10  NFVNDTCNKLVQYDQLYKLCIPEIESDPREDLK-NLTGLLILFVNHSISDFNATISFLHK 69
           NFV+DTCNK  QYD+LYK+CIPEI+SDPREDLK NLTG LI+FVNH+IS+FN  I FL +
Sbjct: 14  NFVHDTCNKF-QYDELYKVCIPEIDSDPREDLKSNLTGFLIIFVNHTISNFNDNIVFLQR 73

Query: 70  EIISGKLDRDTKDMHESRSEDYNMGLDDLHETVHRLLTKQSTDVQKL-VNHADTVIGGCE 129
           EI SGKL+ +TK M+ +  + + MG  +L E++H LLT+  T+V +L     D  I  C 
Sbjct: 74  EIQSGKLNAETKLMYNTCLKAFGMGYSNLQESMHILLTQPGTNVYELPTMDVDKRISTCV 133

Query: 130 DDFDAYPWSLEWKSRYKTSYALLNLSLAMINLIK-CN 164
           DDFD  P   EW+SRY TS+ L+ L +   NLI  CN
Sbjct: 134 DDFDGEPIPPEWQSRYNTSWNLVALMVETSNLITYCN 169

BLAST of MS019981 vs. NCBI nr
Match: KAG6572388.1 (hypothetical protein SDJN03_29116, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 154.5 bits (389), Expect = 8.4e-34
Identity = 83/169 (49.11%), Postives = 113/169 (66.86%), Query Frame = 0

Query: 2   DQTNTTINNFVNDTCNKLVQYDQLYKLCIPEIESDPREDLK-NLTGLLILFVNHSISDFN 61
           ++  + I+ FV+DTCN+    ++LY LCI EI+SDPREDLK NLTG+L +FVNH+I++FN
Sbjct: 6   EENRSNIDKFVHDTCNRFPD-NELYNLCISEIDSDPREDLKFNLTGILTIFVNHTINNFN 65

Query: 62  ATISFLHKEIISGKLDRDTKDMHESRSEDYNMGLDDLHETVHRLLTKQSTDVQKL-VNHA 121
             + FL +EI S KL+ +TK M++  SE + MG   L E++H LLT+  T+V  L     
Sbjct: 66  DNLVFLQQEIKSEKLEAETKLMYDKCSESFGMGYTKLQESMHILLTQPGTNVYDLPTMSV 125

Query: 122 DTVIGGCEDDFDAYPWSLEWKSRYKTSYALLNLSLAMINLIKCNHVEGC 169
           D  I  C DDF+  P   EW+SRYKTS+ L++L L   NLIKCNH   C
Sbjct: 126 DKRISVCFDDFEDDPVPPEWQSRYKTSWNLVSLILGTSNLIKCNHEASC 173

BLAST of MS019981 vs. ExPASy TrEMBL
Match: A0A5D3BJB5 (Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004170 PE=4 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 7.6e-33
Identity = 84/166 (50.60%), Postives = 108/166 (65.06%), Query Frame = 0

Query: 5   NTTINNFVNDTCNKLVQYDQLYKLCIPEIESDPREDLK-NLTGLLILFVNHSISDFNATI 64
           NTTIN+FV  TCN+     +L  LC+ +I S+PR DLK NLTGLL++FVN SIS+F   I
Sbjct: 25  NTTINDFVRHTCNRT---HELKNLCLSQISSEPRNDLKSNLTGLLMIFVNQSISNFKNDI 84

Query: 65  SFLHKEIISGKLDRDTKDMHESRSEDYNMGLDDLHETVHRLLTKQSTDVQKL-VNHADTV 124
           SFL KEI S K+ RDTKDM E   +++ +G  +L ET+  L TK   D ++L V +   +
Sbjct: 85  SFLEKEINSNKISRDTKDMLEDCLQNFQIGSVNLRETMEILQTKTGYDAEQLPVGNVGNL 144

Query: 125 IGGCEDDFDAYPWSLEWKSRYKTSYALLNLSLAMINLIKCNHVEGC 169
              C DDF+  P   EW+SRY  S+ALL LS+AMINLI CN    C
Sbjct: 145 AVECFDDFEGTPVPPEWQSRYNASFALLKLSMAMINLITCNRDYAC 187

BLAST of MS019981 vs. ExPASy TrEMBL
Match: A0A6J1C7W5 (uncharacterized protein LOC111008804 OS=Momordica charantia OX=3673 GN=LOC111008804 PE=4 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 2.0e-20
Identity = 61/161 (37.89%), Postives = 89/161 (55.28%), Query Frame = 0

Query: 9   NNFVNDTCNKLVQYDQLYKLCIPEIESDPREDLK-NLTGLLILFVNHSISDFNATISFLH 68
           N+FVN+TCNK    ++  K+CI  I+SDP +DLK NL+GLL +F+NHSI +      +L 
Sbjct: 11  NSFVNNTCNKF--EEEFIKVCIDVIDSDPHQDLKLNLSGLLAIFINHSIDNATDNHLYLM 70

Query: 69  KEIISGKLDRDTKDMHESRSEDYNMGLDDLHETVHRLLTKQSTDVQKLVNHADTVIGGCE 128
           +++    LD+D +       E Y  GLD L  ++  LL     D+  +++ A   I  CE
Sbjct: 71  EQLKRSDLDKDIRYTFNVCVEQYERGLDILKSSLEALLKNDKDDLGLILSDAFDKIVACE 130

Query: 129 DDFDAYPWSLEWKSRYKTSYALLNLSLAMINLIKCNHVEGC 169
           DDF++ P    W + Y     LL LS+   NLI+C  V  C
Sbjct: 131 DDFESTPEPPSWLAHYLPLRKLLALSMGTANLIQCKKVAAC 169

BLAST of MS019981 vs. ExPASy TrEMBL
Match: A0A2K2A1V6 (PMEI domain-containing protein OS=Populus trichocarpa OX=3694 GN=POPTR_006G134900 PE=4 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 2.3e-05
Identity = 36/125 (28.80%), Postives = 64/125 (51.20%), Query Frame = 0

Query: 8   INNFVNDTCNKLVQYDQLYKLCIPEIESDPREDLKNLTGLLILFVNHSISDFNATISFLH 67
           I N ++ TCN  + ++    +C+  + SDPR    +L GL  + +N SI+  + T++FL 
Sbjct: 37  IRNLISATCNHTLYFE----MCVSALRSDPRSQTSDLVGLANIALNISIAHGSETLAFL- 96

Query: 68  KEIISGKLDRDTK--DMHESRSEDYNMGLDDLHETVHRLLTKQSTDVQKLVNHADTVIGG 127
            +++      DT+   +    +E+Y  G ++L E +H L  +   D+  LV+ A T    
Sbjct: 97  -KVLKSNAGNDTQLSGILSECTEEYIEGTENLEEAIHALRIRSFDDMNTLVSTAMTDSDT 155

Query: 128 CEDDF 131
           CE  F
Sbjct: 157 CEQGF 155

BLAST of MS019981 vs. ExPASy TrEMBL
Match: A0A443P2W9 (Putative invertase inhibitor OS=Cinnamomum micranthum f. kanehirae OX=337451 GN=CKAN_01397200 PE=4 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 5.7e-04
Identity = 36/121 (29.75%), Postives = 60/121 (49.59%), Query Frame = 0

Query: 10  NFVNDTCNKLVQYDQLYKLCIPEIESDPREDLKNLTGLLILFVNHSISDFNATISFLHKE 69
           + + +TC K  + +  Y LCI  +ESDPR    +L GL  + +  ++++   T S + + 
Sbjct: 16  SLIQETCKKASRVN--YTLCITALESDPRSPTTDLAGLGSISIQLALANATQTWSHIQEM 75

Query: 70  IISGKLDRDTKDMHESRSEDYNMGLDDLHETVHRLLTKQSTDVQKLVNHADTVIGGCEDD 129
           + SG+ D   K   E   E Y+  + +L E++  +  K   DV   V+ A T    CED 
Sbjct: 76  LKSGRFDSYDKARLEDCLELYSEAISNLTESLKAMKGKSYLDVNVRVSAAMTDSVTCEDG 134

Query: 130 F 131
           F
Sbjct: 136 F 134

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023511468.13.1e-3651.48uncharacterized protein LOC111776286 [Cucurbita pepo subsp. pepo][more]
KAG6572151.15.8e-3551.76putative pectinesterase/pectinesterase inhibitor 7, partial [Cucurbita argyrospe... [more]
KAG6572152.17.6e-3549.09Expansin-like A2, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG6572380.13.8e-3454.78ATP-dependent zinc metalloprotease FTSH 2, chloroplastic, partial [Cucurbita arg... [more]
KAG6572388.18.4e-3449.11hypothetical protein SDJN03_29116, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5D3BJB57.6e-3350.60Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A6J1C7W52.0e-2037.89uncharacterized protein LOC111008804 OS=Momordica charantia OX=3673 GN=LOC111008... [more]
A0A2K2A1V62.3e-0528.80PMEI domain-containing protein OS=Populus trichocarpa OX=3694 GN=POPTR_006G13490... [more]
A0A443P2W95.7e-0429.75Putative invertase inhibitor OS=Cinnamomum micranthum f. kanehirae OX=337451 GN=... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 7..156
e-value: 1.0E-4
score: 31.7
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 9..158
e-value: 2.0E-15
score: 54.6
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 10..153
e-value: 5.6E-10
score: 39.7
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 7..160
e-value: 5.1E-15
score: 57.8
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 9..160

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS019981.1MS019981.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004857 enzyme inhibitor activity