MS019917 (gene) Bitter gourd (TR) v1

Overview


Name	MS019917
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	F-box/LRR-repeat protein At5g63520
Location	scaffold22: 549264 .. 549515 (+)
RNA-Seq Expression	MS019917
Synteny	MS019917

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAAGGCCATCGGAGCACGCGGAGTTACCGCCGGAGGATCTTTTCCGGTGAACGGCGACATTCTCCATAACATATTGGGAAGACTTCCGGCCCGGTGTTTCGCGTCGGCGGCGTGCGTGTGTAAAACATGGAACTGTATCTGCAACCGTATCCTGTCTCGCCCTAAATTCGCCTCCGCCGTCTCCCTGAATCCCTCGTTCGACGTTATCCTTCTTCCTCTCCTCTTTATTTCGCCAATTTTACTTAAT

mRNA sequence

ATGGAGAAGGCCATCGGAGCACGCGGAGTTACCGCCGGAGGATCTTTTCCGGTGAACGGCGACATTCTCCATAACATATTGGGAAGACTTCCGGCCCGGTGTTTCGCGTCGGCGGCGTGCGTGTGTAAAACATGGAACTGTATCTGCAACCGTATCCTGTCTCGCCCTAAATTCGCCTCCGCCGTCTCCCTGAATCCCTCGTTCGACGTTATCCTTCTTCCTCTCCTCTTTATTTCGCCAATTTTACTTAAT

Coding sequence (CDS)

ATGGAGAAGGCCATCGGAGCACGCGGAGTTACCGCCGGAGGATCTTTTCCGGTGAACGGCGACATTCTCCATAACATATTGGGAAGACTTCCGGCCCGGTGTTTCGCGTCGGCGGCGTGCGTGTGTAAAACATGGAACTGTATCTGCAACCGTATCCTGTCTCGCCCTAAATTCGCCTCCGCCGTCTCCCTGAATCCCTCGTTCGACGTTATCCTTCTTCCTCTCCTCTTTATTTCGCCAATTTTACTTAAT

Protein sequence

MEKAIGARGVTAGGSFPVNGDILHNILGRLPARCFASAACVCKTWNCICNRILSRPKFASAVSLNPSFDVILLPLLFISPILLN

Homology

[Close]

BLAST of MS019917 vs. NCBI nr
Match: XP_022137117.1 (F-box/LRR-repeat protein At5g63520 [Momordica charantia])

HSP 1 Score: 149.1 bits (375), Expect = 1.8e-32
Identity = 71/76 (93.42%), Postives = 73/76 (96.05%), Query Frame = 0


Query: 1  MEKAIGARGVTAGGSFPVNGDILHNILGRLPARCFASAACVCKTWNCICNRILSRPKFAS 60
          MEKAIGARGVTAGGSFPVNGDILHNILGRLPARCFASAACVCKTWNCICNRILSRPKFAS
Sbjct: 1  MEKAIGARGVTAGGSFPVNGDILHNILGRLPARCFASAACVCKTWNCICNRILSRPKFAS 60

Query: 61 AVSLNPSFDVILLPLL 77
          AVSLNPSFDV +  +L
Sbjct: 61 AVSLNPSFDVAVKEVL 76

[Close]

BLAST of MS019917 vs. NCBI nr
Match: KAG6573137.1 (F-box/LRR-repeat protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 103.6 bits (257), Expect = 8.5e-19
Identity = 53/79 (67.09%), Postives = 58/79 (73.42%), Query Frame = 0


Query: 1  MEKAIGARGVTAGGS---FPVNGDILHNILGRLPARCFASAACVCKTWNCICNRILSRPK 60
          ME  IGA GV  GG     P+N DIL NILGRLPAR FASA CVCKTWN +CNRIL RPK
Sbjct: 1  METVIGAGGVDGGGGGGISPLNDDILRNILGRLPARSFASAGCVCKTWNSVCNRILCRPK 60

Query: 61 FASAVSLNPSFDVILLPLL 77
          F+SAVSLNPS  V +  +L
Sbjct: 61 FSSAVSLNPSLHVAVEEVL 79

[Close]

BLAST of MS019917 vs. NCBI nr
Match: XP_022955211.1 (F-box/LRR-repeat protein At5g63520-like [Cucurbita moschata])

HSP 1 Score: 103.6 bits (257), Expect = 8.5e-19
Identity = 53/79 (67.09%), Postives = 58/79 (73.42%), Query Frame = 0


Query: 1  MEKAIGARGVTAGGS---FPVNGDILHNILGRLPARCFASAACVCKTWNCICNRILSRPK 60
          ME  IGA GV  GG     P+N DIL NILGRLPAR FASA CVCKTWN +CNRIL RPK
Sbjct: 1  METVIGAGGVDGGGGGGISPLNDDILRNILGRLPARSFASAGCVCKTWNSVCNRILCRPK 60

Query: 61 FASAVSLNPSFDVILLPLL 77
          F+SAVSLNPS  V +  +L
Sbjct: 61 FSSAVSLNPSLHVAVEEVL 79

[Close]

BLAST of MS019917 vs. NCBI nr
Match: XP_022994367.1 (F-box/LRR-repeat protein At5g63520-like [Cucurbita maxima])

HSP 1 Score: 103.2 bits (256), Expect = 1.1e-18
Identity = 53/80 (66.25%), Postives = 59/80 (73.75%), Query Frame = 0


Query: 1  MEKAIGARGVTAGGSF----PVNGDILHNILGRLPARCFASAACVCKTWNCICNRILSRP 60
          ME  IGA GV  GG      P+N DIL NI+GRLPAR FASAACVCKTWN +CNRIL RP
Sbjct: 1  METVIGAGGVGGGGGGGGIPPLNDDILRNIIGRLPARSFASAACVCKTWNSVCNRILCRP 60

Query: 61 KFASAVSLNPSFDVILLPLL 77
          KF+SAVSLNPS  V +  +L
Sbjct: 61 KFSSAVSLNPSLHVAVEEVL 80

[Close]

BLAST of MS019917 vs. NCBI nr
Match: XP_023542621.1 (F-box/LRR-repeat protein At5g63520-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 102.8 bits (255), Expect = 1.4e-18
Identity = 54/84 (64.29%), Postives = 59/84 (70.24%), Query Frame = 0


Query: 1  MEKAIGARGVTAGGS--------FPVNGDILHNILGRLPARCFASAACVCKTWNCICNRI 60
          ME  IGA GV  GG          P+N DIL NILGRLPAR FASAACVCKTWN +CNRI
Sbjct: 1  METVIGAGGVGGGGGGGGGGGGISPLNDDILRNILGRLPARSFASAACVCKTWNSVCNRI 60

Query: 61 LSRPKFASAVSLNPSFDVILLPLL 77
          L RPKF+SAVSLNPS  V +  +L
Sbjct: 61 LCRPKFSSAVSLNPSLHVAVEEVL 84

[Close]

BLAST of MS019917 vs. ExPASy Swiss-Prot
Match: Q9FMV0 (F-box/LRR-repeat protein At5g63520 OS=Arabidopsis thaliana OX=3702 GN=At5g63520 PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 5.2e-11
Identity = 31/49 (63.27%), Postives = 38/49 (77.55%), Query Frame = 0


Query: 18 VNGDILHNILGRLPARCFASAACVCKTWNCICNRILSRPKFASAVSLNP 67
          +N D+LHNIL RLPA+ FA A+CV + W+ +CNRILSRPK  SA S NP
Sbjct: 36 MNEDLLHNILLRLPAKSFAFASCVNRFWSSVCNRILSRPKMISAFSRNP 84

[Close]

BLAST of MS019917 vs. ExPASy TrEMBL
Match: A0A6J1C7B9 (F-box/LRR-repeat protein At5g63520 OS=Momordica charantia OX=3673 GN=LOC111008671 PE=4 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 8.5e-33
Identity = 71/76 (93.42%), Postives = 73/76 (96.05%), Query Frame = 0


Query: 1  MEKAIGARGVTAGGSFPVNGDILHNILGRLPARCFASAACVCKTWNCICNRILSRPKFAS 60
          MEKAIGARGVTAGGSFPVNGDILHNILGRLPARCFASAACVCKTWNCICNRILSRPKFAS
Sbjct: 1  MEKAIGARGVTAGGSFPVNGDILHNILGRLPARCFASAACVCKTWNCICNRILSRPKFAS 60

Query: 61 AVSLNPSFDVILLPLL 77
          AVSLNPSFDV +  +L
Sbjct: 61 AVSLNPSFDVAVKEVL 76

[Close]

BLAST of MS019917 vs. ExPASy TrEMBL
Match: A0A6J1GT60 (F-box/LRR-repeat protein At5g63520-like OS=Cucurbita moschata OX=3662 GN=LOC111457244 PE=4 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 4.1e-19
Identity = 53/79 (67.09%), Postives = 58/79 (73.42%), Query Frame = 0


Query: 1  MEKAIGARGVTAGGS---FPVNGDILHNILGRLPARCFASAACVCKTWNCICNRILSRPK 60
          ME  IGA GV  GG     P+N DIL NILGRLPAR FASA CVCKTWN +CNRIL RPK
Sbjct: 1  METVIGAGGVDGGGGGGISPLNDDILRNILGRLPARSFASAGCVCKTWNSVCNRILCRPK 60

Query: 61 FASAVSLNPSFDVILLPLL 77
          F+SAVSLNPS  V +  +L
Sbjct: 61 FSSAVSLNPSLHVAVEEVL 79

[Close]

BLAST of MS019917 vs. ExPASy TrEMBL
Match: A0A6J1JVL6 (F-box/LRR-repeat protein At5g63520-like OS=Cucurbita maxima OX=3661 GN=LOC111490107 PE=4 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 5.4e-19
Identity = 53/80 (66.25%), Postives = 59/80 (73.75%), Query Frame = 0


Query: 1  MEKAIGARGVTAGGSF----PVNGDILHNILGRLPARCFASAACVCKTWNCICNRILSRP 60
          ME  IGA GV  GG      P+N DIL NI+GRLPAR FASAACVCKTWN +CNRIL RP
Sbjct: 1  METVIGAGGVGGGGGGGGIPPLNDDILRNIIGRLPARSFASAACVCKTWNSVCNRILCRP 60

Query: 61 KFASAVSLNPSFDVILLPLL 77
          KF+SAVSLNPS  V +  +L
Sbjct: 61 KFSSAVSLNPSLHVAVEEVL 80

[Close]

BLAST of MS019917 vs. ExPASy TrEMBL
Match: A0A6J1KFR0 (LOW QUALITY PROTEIN: F-box/LRR-repeat protein At5g63520-like OS=Cucurbita maxima OX=3661 GN=LOC111495385 PE=4 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 6.8e-14
Identity = 41/53 (77.36%), Postives = 43/53 (81.13%), Query Frame = 0


Query: 18 VNGDILHNILGRLPARCFASAACVCKTWNCICNRILSRPKFASAVSLNPSFDV 71
          +N DILHNI  RLPAR FA+AACV KTWN  CNRIL RPKFASAVSLNPS  V
Sbjct: 1  MNDDILHNIFRRLPARSFAAAACVSKTWNSTCNRILRRPKFASAVSLNPSLHV 53

[Close]

BLAST of MS019917 vs. ExPASy TrEMBL
Match: A0A6J1EDN6 (F-box/LRR-repeat protein At5g63520-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431588 PE=4 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 6.8e-14
Identity = 41/53 (77.36%), Postives = 43/53 (81.13%), Query Frame = 0


Query: 18 VNGDILHNILGRLPARCFASAACVCKTWNCICNRILSRPKFASAVSLNPSFDV 71
          +N DILHNI  RLPAR FA+AACV KTWN  CNRIL RPKFASAVSLNPS  V
Sbjct: 1  MNDDILHNIFRRLPARSFAAAACVSKTWNSTCNRILRRPKFASAVSLNPSLHV 53

[Close]

BLAST of MS019917 vs. TAIR 10
Match: AT5G63520.1 (CONTAINS InterPro DOMAIN/s: F-box domain, Skp2-like (InterPro:IPR022364), FIST C domain (InterPro:IPR019494), FIST domain, N-terminal (InterPro:IPR013702); Has 137 Blast hits to 137 proteins in 56 species: Archae - 0; Bacteria - 88; Metazoa - 6; Fungi - 0; Plants - 32; Viruses - 0; Other Eukaryotes - 11 (source: NCBI BLink). )

HSP 1 Score: 68.2 bits (165), Expect = 3.7e-12
Identity = 31/49 (63.27%), Postives = 38/49 (77.55%), Query Frame = 0


Query: 18 VNGDILHNILGRLPARCFASAACVCKTWNCICNRILSRPKFASAVSLNP 67
          +N D+LHNIL RLPA+ FA A+CV + W+ +CNRILSRPK  SA S NP
Sbjct: 26 MNEDLLHNILLRLPAKSFAFASCVNRFWSSVCNRILSRPKMISAFSRNP 74

The following BLAST results are available for this feature:

BLAST of MS019917 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022137117.1	1.8e-32	93.42	F-box/LRR-repeat protein At5g63520 [Momordica charantia]	[more]
KAG6573137.1	8.5e-19	67.09	F-box/LRR-repeat protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022955211.1	8.5e-19	67.09	F-box/LRR-repeat protein At5g63520-like [Cucurbita moschata]	[more]
XP_022994367.1	1.1e-18	66.25	F-box/LRR-repeat protein At5g63520-like [Cucurbita maxima]	[more]
XP_023542621.1	1.4e-18	64.29	F-box/LRR-repeat protein At5g63520-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS019917 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FMV0	5.2e-11	63.27	F-box/LRR-repeat protein At5g63520 OS=Arabidopsis thaliana OX=3702 GN=At5g63520 ...	[more]

BLAST of MS019917 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C7B9	8.5e-33	93.42	F-box/LRR-repeat protein At5g63520 OS=Momordica charantia OX=3673 GN=LOC11100867...	[more]
A0A6J1GT60	4.1e-19	67.09	F-box/LRR-repeat protein At5g63520-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1JVL6	5.4e-19	66.25	F-box/LRR-repeat protein At5g63520-like OS=Cucurbita maxima OX=3661 GN=LOC111490...	[more]
A0A6J1KFR0	6.8e-14	77.36	LOW QUALITY PROTEIN: F-box/LRR-repeat protein At5g63520-like OS=Cucurbita maxima...	[more]
A0A6J1EDN6	6.8e-14	77.36	F-box/LRR-repeat protein At5g63520-like isoform X1 OS=Cucurbita moschata OX=3662...	[more]

BLAST of MS019917 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G63520.1	3.7e-12	63.27	CONTAINS InterPro DOMAIN/s: F-box domain, Skp2-like (InterPro:IPR022364), FIST C...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001810	F-box domain	PFAM	PF00646	F-box	coord: 21..58 e-value: 5.4E-7 score: 29.3
None	No IPR available	GENE3D	1.20.1280.50		coord: 19..55 e-value: 2.1E-6 score: 29.5
None	No IPR available	PANTHER	PTHR14939	F-BOX ONLY PROTEIN 22	coord: 16..70
None	No IPR available	PANTHER	PTHR14939:SF8	SKP2-LIKE, FIST C DOMAIN, FIST DOMAIN, N-TERMINAL ISOFORM 1	coord: 16..70
IPR036047	F-box-like domain superfamily	SUPERFAMILY	81383	F-box domain	coord: 20..55

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS019917.1	MS019917.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005515	protein binding