MS019763 (gene) Bitter gourd (TR) v1

Overview
NameMS019763
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionDelta-1-pyrroline-5-carboxylate synthase
Locationscaffold729: 2026463 .. 2030966 (-)
RNA-Seq ExpressionMS019763
SyntenyMS019763
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTCATCCAACCAGAGTTTTTGTCAAAAATGTCAAGCGTTTGATTGTTAAGGTACTCTTTTTTATGCGTCCAAATCTTTATGTTTAATGATCCAGAAATCATGTTCTTTTATCTGTTTTCTAATTCATTTTTACCATTTGCAACTTTTGTCTCTCAAAAATATTAAATATATATAACTGATCCTATGTTTGAAAAACTTCTTTGATATCTATTTCTCAATTAATATTAATAGTCCTGTCTTGGATCCTGTATTAAAAATTAGTTATATGGTAACCTTTTTGCTTAATCTTTTGGGTTTGATTGCTTGTGTGTGATATTTATCTTTTTCATTGTAATTTATTAGTAGTTCCTAACCATACGAGCTTGTTTCGTGAATAAGACATCTATTATCATCCTGAAGAATTGAGAGTTGCATCCTCATCCCAAAAAAAAACCACATTGATTTCTTATGGCTCTTTATTTATTTTTGTAAGGTGGGAACGGCTGTGGTTACTCGTGGAGATGGACGGCTGGCATTGGGAAGGCTGGGAGCTCTGTGTGAGCAGGTGATTAATTAGTTTGGTTATTTGGGAAATTTTGTTGCATTAATTTGATGCTAATTGATTGTGAAACATTAATGGCTGGACAGCTCAAGGAGTTGAACTCTCGAGGATATGAGGTTATTCTGGTTACATCAGGTGCTGTAGGTCTTGGTCGTCAAAGGCTCAGATATAGAAGATTGGCCAATAGCAGGTTCTTAATACCTTCCCTTTTCTGCCTTCTTTTTGAGACATTCTGTTTCAGGTTTAGATTCTTATATATACCATCGGCGGATGGTGGCATAGTGGGCTTGTGGCCGAATGGGGATACCCCTCCACAGTTCGAGCCCTGCGGGGAGTGTAGCAGGGGCCTTTCCTCCTATTCGAGGACGACTCTAGCAACTTCGGTTACAGTGTTGGGTGGCCGACACCCCTAGTTATAGGGATTCAACTAGAAGCCACACAGCATCAATTCCGGGTTATAAAAAAAGGTTTAGATTCTGATATATATGTATGTTTTTGTTTCTCCACAGCTTCGCTGATCTTCAGAATCCACAAGGTGATTTTGATGGGAAGGCTTGTGCAGCTGTTGGGCAGAGTAGCCTCATGGCTCTTTATGATACCATGTTTAGCCAGGTAGAATACAAATTATGATCTAAACTACTCATAGGAATGCAAATTTCTTTTGAATACCAAACAATTTTGGCTCCAATTGTTGTTGCTAATCTAATGATTTCTTATGCAATTTGCAGCTTGATGTGACCTCTTCCCAACTTCTAGTGACAGATTCAGTTTTTTGCGACTCTGGCTTTAGACAGCAGCTTTCTGAGACAGTTAACTCATTGTTAGATCTTAGGGTCATCCCCATTTTTAATGAAAATGATGCAGTTAGTACTAGGAAAACACCATATGAGGTATTGTCATTTTTTATGCATATTTTTCCTACCCATCTTTTCAAATGTCTGTAAGATTCTGATCAAGAGCAACTTTTTATCAGGATTCTTCTGGTATTTTCTGGGATAATGACAGTTTGGCAGGTCTATTAGCTTTAGAACTCAAAGCTGATCTTCTGGTGTTACTCAGTGATGTTGAAGGCCTGTATAGTGGCCCTCCATGTGACCCGAATTCGAAGCTAATCCGGACTTTTATTAAAGAGAAACATTTAGGGGAAATAACATTTGGTGACAAGTCCAGGTTGGGCAGAGGTGGTATGACTGCTAAAGTAAATGCTGCTGTTTATGCTGCTTCTGCTGGAATTCCTGTTGTCATTACTAGGTATATGATCCATCTTGTCACTTGACCATTTTGAAGTCTTTGATACATCTATTGTAACTCCATCTCTAATTCCCAATCTGGTGCAGTGGTTTTGCCACTGATAACATAATAAAAGTACTTCAAGGTGAGATGAAAGGAACTCTCTTTCACAAAGATGCCCATTTGTGGACTGTGGTTAAAGAAATTTCTGCAAGAGATATGGCAGTTTCGGCCCGGGAAAGTTCGAGGCGGCTTCAGGTAGTGAAATGGAACAAGTTTCTGTTATTACAACTCCTGGATTCATTAATTTCTCATATTTGTTGTATGAGTTTGGATTCCTCATGATTTTAAACGTTTGTATTGGTATGCAGGCTCTGAAATCTGAAGATAGGAGGAAGATATTGCTGGATGTGGCTGATGCCCTTGAAGATAATGAAGATATGATTATTGCTGAGAACCTTGCTGATATTAAAGCCGCGGAGGAGGTTGGATATGAGAAGTCTCTGATATCTCGTCTGGCTCTAAAGCCTGGAAGGGTACTTTAAGATCCTTTGATAAATGATAAGTCATGCTTCTCTTTTTCTATAGAAAATTTAGCAGAAACAATTTTTGTTTTACAGATTGGGACTCTTGCAAGCTCTATTCGTAAACTTGCAGACATGGAAGAGCCAATAGGTCGCATTTTAAAAAGAAAAGAAGTAGGAATACTGTTTCAAATTTAGTTTTTTTTCTTTCTCTGTTCTCTGTTATATTTGAGTTCATTGATACTCCCTCTACTATGCCATCGCTTCGTTGAGCAGTTTTAATGATTTATCTACCACTTCATTCAGCTTGCTGATGACCTTATTTTAGAAAACACCTCGTGCTCTTTGGGCGTCCTCCTCATCGTGTTCGAGTCTCGGCCAGATGCTCTAGTTCAGGTGAGGATGAACATCCAATGCTTCCTACTTGTTATAAGTGCTAGAATTGGAGCACATTGTGATTAGTTTTCTACTGCTTTTACAGATAGCTGCACTGGCGATACGAAGCGGAAACGGTTTACTCCTTAAAGGGGGGAAAGAAGCCAGGCGATCAAATGCAGCTCTGCATAAGGTTCATTTTCCTTTGCCAGCTTATTCTAGATTGATTGATTGTTCCTGTTTCCATGTTTCTTTTGGTTAAGTTATCGATTCCTTCTTTCCTTAGGTCATCACTTCAGCCATCCCCGATACCGTCGGTGAAAAACTTATTGGACTCATAACATCAAGGGAAGAGATTCCTGATCTGCTCAAGGTGAAGGGAGGAGAGAATTTGTTTATACTTAGTGTTGCTTATAAGCTCTGAGTGAATACTGAATATGAATATATCTGGGTTATTTTCTTATAACAACTTGATGCCACAGCTTGATGATGTTATAGACCTTGTGATTCCAAGAGGAAGTAACAAACTTGTGTCTCAGATTAAAGAGTCCACAAAGATTCCTGTTCTTGGTCATTCAGGTGAGAAATAACATGCAGCAATATGCCATAGTCTTAAAACCAGATCTTATACTACTGAAAGTTCTTTATTTTCCAACAACACAGATGGGATCTGTCACGTTTATGTCGACAAATCAGCGGATATAGAGATGGCGAAGCGGATAGTATCTGATGCTAAGGCAGATTATCCAGCAGCCTGCAACGCAATGGTTTGTCCACTTCTTGGTGTTGATAGCAGAACTATTTACAGTCTGCAAAATGTTCTTATCTTTTTGGTTATAATTTGCAGTCCCTTTTATGCCTTTTCAGGAAACGCTCCTGGTGCACAAGGATTTGGCAAGTAACGGAATGCTTATCGAACTCGTTACGGAACTGCGAAAGGAAGGTATAGATGTACCATTTCTTCTTCATCAAACTGAAAAGTGAAAACTGCTGTCACTAAGGAAAAAATTGGTTTGTAATGTAGGTGTGACTCTATACGGGGGAAAACGAGCTAGCGCAATACTTAAGATCAAAGAGGCAAGTTCTTTCCACCATGAGTACAATTCACTGGCTTGCACTGTTGAAATTGTGGATGATGTCTTTTCTGCCATTGATCATATACATGAACATGGAAGGTGAGCCAGAAAACAAAATTTCCTATATGTTAGTATGGAACTAATGAACTCAAATTGACCGACCACTCTTTCGGCAGCGCACATACTGACTGTGTCGTCGCTGAAGATCAGGAAGTTGCTGAAATTTTCTTGAATCAAGTTGATAGGTACGATGATAGCTTTGCTAAAAAAACACTTGTAATGTAACTCAACTTTTTGGTCCAAGAGCCTGATTTTTATGTGAACCATCTCTAATTCTCTCAATCTTGTATGCAACAGTGCTGCAGTTATCCACAATGCTAGTACACGTTTCTGCGACGGAGCTCGATTCGGACTTGGTGCAGAGGTAAGCTATTCCCTAACCAATATTAACCCATCCGAATAAGCTTTTGGGTTGATTGATGATTCAACGCTGGCTGTTTATGACAAAACAGGTGGGAATAAGTACTGGTAGGATTCATGCTCGAGGCCCAGTGGGGGTAGAAGGACTATTGACTACGAGATGGTAATACGACATTCTGAATATAATGTTAAAAGGAATATGATTAGGCAAATGCCAAATGTAGGATTGATTTGGTGTTGATGTTTGTGTAAATGCAGGATCTTAAGGGGGAGTGGGCAAGTAGTGAATGGTGATAAAGGAGTGGCCTACACCCATAAGGATCTTGGGGTGGCCATG

mRNA sequence

GTTCATCCAACCAGAGTTTTTGTCAAAAATGTCAAGCGTTTGATTGTTAAGGTGGGAACGGCTGTGGTTACTCGTGGAGATGGACGGCTGGCATTGGGAAGGCTGGGAGCTCTGTGTGAGCAGCTCAAGGAGTTGAACTCTCGAGGATATGAGGTTATTCTGGTTACATCAGGTGCTGTAGGTCTTGGTCGTCAAAGGCTCAGATATAGAAGATTGGCCAATAGCAGCTTCGCTGATCTTCAGAATCCACAAGGTGATTTTGATGGGAAGGCTTGTGCAGCTGTTGGGCAGAGTAGCCTCATGGCTCTTTATGATACCATGTTTAGCCAGCTTGATGTGACCTCTTCCCAACTTCTAGTGACAGATTCAGTTTTTTGCGACTCTGGCTTTAGACAGCAGCTTTCTGAGACAGTTAACTCATTGTTAGATCTTAGGGTCATCCCCATTTTTAATGAAAATGATGCAGTTAGTACTAGGAAAACACCATATGAGGATTCTTCTGGTATTTTCTGGGATAATGACAGTTTGGCAGGTCTATTAGCTTTAGAACTCAAAGCTGATCTTCTGGTGTTACTCAGTGATGTTGAAGGCCTGTATAGTGGCCCTCCATGTGACCCGAATTCGAAGCTAATCCGGACTTTTATTAAAGAGAAACATTTAGGGGAAATAACATTTGGTGACAAGTCCAGGTTGGGCAGAGGTGGTATGACTGCTAAAGTAAATGCTGCTGTTTATGCTGCTTCTGCTGGAATTCCTGTTGTCATTACTAGGTATATGATCCATCTTGTCACTCATTTTGAAGTCTTTGATACATCTATTGTAACTCCATCTCTAATTCCCAATCTGGTGAGTGGTTTTGCCACTGATAACATAATAAAAGTACTTCAAGGTGAGATGAAAGGAACTCTCTTTCACAAAGATGCCCATTTGTGGACTGTGGTTAAAGAAATTTCTGCAAGAGATATGGCAGTTTCGGCCCGGGAAAGTTCGAGGCGGCTTCAGGCTCTGAAATCTGAAGATAGGAGGAAGATATTGCTGGATGTGGCTGATGCCCTTGAAGATAATGAAGATATGATTATTGCTGAGAACCTTGCTGATATTAAAGCCGCGGAGGAGGTTGGATATGAGAAGTCTCTGATATCTCGTCTGGCTCTAAAGCCTGGAAGGATTGGGACTCTTGCAAGCTCTATTCGTAAACTTGCAGACATGGAAGAGCCAATAGGTCGCATTTTAAAAAGAAAAGAACTTGCTGATGACCTTATTTTAGAAAACACCTCGTGCTCTTTGGGCGTCCTCCTCATCGTGTTCGAGTCTCGGCCAGATGCTCTAGTTCAGATAGCTGCACTGGCGATACGAAGCGGAAACGGTTTACTCCTTAAAGGGGGGAAAGAAGCCAGGCGATCAAATGCAGCTCTGCATAAGGTCATCACTTCAGCCATCCCCGATACCGTCGGTGAAAAACTTATTGGACTCATAACATCAAGGGAAGAGATTCCTGATCTGCTCAAGCTTGATGATGTTATAGACCTTGTGATTCCAAGAGGAAGTAACAAACTTGTGTCTCAGATTAAAGAGTCCACAAAGATTCCTGTTCTTGGTCATTCAGATGGGATCTGTCACGTTTATGTCGACAAATCAGCGGATATAGAGATGGCGAAGCGGATAGTATCTGATGCTAAGGCAGATTATCCAGCAGCCTGCAACGCAATGGAAACGCTCCTGGTGCACAAGGATTTGGCAAGTAACGGAATGCTTATCGAACTCGTTACGGAACTGCGAAAGGAAGGTGTGACTCTATACGGGGGAAAACGAGCTAGCGCAATACTTAAGATCAAAGAGGCAAGTTCTTTCCACCATGAGTACAATTCACTGGCTTGCACTGTTGAAATTGTGGATGATGTCTTTTCTGCCATTGATCATATACATGAACATGGAAGCGCACATACTGACTGTGTCGTCGCTGAAGATCAGGAAGTTGCTGAAATTTTCTTGAATCAAGTTGATAGTGCTGCAGTTATCCACAATGCTAGTACACGTTTCTGCGACGGAGCTCGATTCGGACTTGGTGCAGAGGTGGGAATAAGTACTGGTAGGATTCATGCTCGAGGCCCAGTGGGGGTAGAAGGACTATTGACTACGAGATGGATCTTAAGGGGGAGTGGGCAAGTAGTGAATGGTGATAAAGGAGTGGCCTACACCCATAAGGATCTTGGGGTGGCCATG

Coding sequence (CDS)

GTTCATCCAACCAGAGTTTTTGTCAAAAATGTCAAGCGTTTGATTGTTAAGGTGGGAACGGCTGTGGTTACTCGTGGAGATGGACGGCTGGCATTGGGAAGGCTGGGAGCTCTGTGTGAGCAGCTCAAGGAGTTGAACTCTCGAGGATATGAGGTTATTCTGGTTACATCAGGTGCTGTAGGTCTTGGTCGTCAAAGGCTCAGATATAGAAGATTGGCCAATAGCAGCTTCGCTGATCTTCAGAATCCACAAGGTGATTTTGATGGGAAGGCTTGTGCAGCTGTTGGGCAGAGTAGCCTCATGGCTCTTTATGATACCATGTTTAGCCAGCTTGATGTGACCTCTTCCCAACTTCTAGTGACAGATTCAGTTTTTTGCGACTCTGGCTTTAGACAGCAGCTTTCTGAGACAGTTAACTCATTGTTAGATCTTAGGGTCATCCCCATTTTTAATGAAAATGATGCAGTTAGTACTAGGAAAACACCATATGAGGATTCTTCTGGTATTTTCTGGGATAATGACAGTTTGGCAGGTCTATTAGCTTTAGAACTCAAAGCTGATCTTCTGGTGTTACTCAGTGATGTTGAAGGCCTGTATAGTGGCCCTCCATGTGACCCGAATTCGAAGCTAATCCGGACTTTTATTAAAGAGAAACATTTAGGGGAAATAACATTTGGTGACAAGTCCAGGTTGGGCAGAGGTGGTATGACTGCTAAAGTAAATGCTGCTGTTTATGCTGCTTCTGCTGGAATTCCTGTTGTCATTACTAGGTATATGATCCATCTTGTCACTCATTTTGAAGTCTTTGATACATCTATTGTAACTCCATCTCTAATTCCCAATCTGGTGAGTGGTTTTGCCACTGATAACATAATAAAAGTACTTCAAGGTGAGATGAAAGGAACTCTCTTTCACAAAGATGCCCATTTGTGGACTGTGGTTAAAGAAATTTCTGCAAGAGATATGGCAGTTTCGGCCCGGGAAAGTTCGAGGCGGCTTCAGGCTCTGAAATCTGAAGATAGGAGGAAGATATTGCTGGATGTGGCTGATGCCCTTGAAGATAATGAAGATATGATTATTGCTGAGAACCTTGCTGATATTAAAGCCGCGGAGGAGGTTGGATATGAGAAGTCTCTGATATCTCGTCTGGCTCTAAAGCCTGGAAGGATTGGGACTCTTGCAAGCTCTATTCGTAAACTTGCAGACATGGAAGAGCCAATAGGTCGCATTTTAAAAAGAAAAGAACTTGCTGATGACCTTATTTTAGAAAACACCTCGTGCTCTTTGGGCGTCCTCCTCATCGTGTTCGAGTCTCGGCCAGATGCTCTAGTTCAGATAGCTGCACTGGCGATACGAAGCGGAAACGGTTTACTCCTTAAAGGGGGGAAAGAAGCCAGGCGATCAAATGCAGCTCTGCATAAGGTCATCACTTCAGCCATCCCCGATACCGTCGGTGAAAAACTTATTGGACTCATAACATCAAGGGAAGAGATTCCTGATCTGCTCAAGCTTGATGATGTTATAGACCTTGTGATTCCAAGAGGAAGTAACAAACTTGTGTCTCAGATTAAAGAGTCCACAAAGATTCCTGTTCTTGGTCATTCAGATGGGATCTGTCACGTTTATGTCGACAAATCAGCGGATATAGAGATGGCGAAGCGGATAGTATCTGATGCTAAGGCAGATTATCCAGCAGCCTGCAACGCAATGGAAACGCTCCTGGTGCACAAGGATTTGGCAAGTAACGGAATGCTTATCGAACTCGTTACGGAACTGCGAAAGGAAGGTGTGACTCTATACGGGGGAAAACGAGCTAGCGCAATACTTAAGATCAAAGAGGCAAGTTCTTTCCACCATGAGTACAATTCACTGGCTTGCACTGTTGAAATTGTGGATGATGTCTTTTCTGCCATTGATCATATACATGAACATGGAAGCGCACATACTGACTGTGTCGTCGCTGAAGATCAGGAAGTTGCTGAAATTTTCTTGAATCAAGTTGATAGTGCTGCAGTTATCCACAATGCTAGTACACGTTTCTGCGACGGAGCTCGATTCGGACTTGGTGCAGAGGTGGGAATAAGTACTGGTAGGATTCATGCTCGAGGCCCAGTGGGGGTAGAAGGACTATTGACTACGAGATGGATCTTAAGGGGGAGTGGGCAAGTAGTGAATGGTGATAAAGGAGTGGCCTACACCCATAAGGATCTTGGGGTGGCCATG

Protein sequence

VHPTRVFVKNVKRLIVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTDSVFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLALELKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHLGEITFGDKSRLGRGGMTAKVNAAVYAASAGIPVVITRYMIHLVTHFEVFDTSIVTPSLIPNLVSGFATDNIIKVLQGEMKGTLFHKDAHLWTVVKEISARDMAVSARESSRRLQALKSEDRRKILLDVADALEDNEDMIIAENLADIKAAEEVGYEKSLISRLALKPGRIGTLASSIRKLADMEEPIGRILKRKELADDLILENTSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGLITSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDAKADYPAACNAMETLLVHKDLASNGMLIELVTELRKEGVTLYGGKRASAILKIKEASSFHHEYNSLACTVEIVDDVFSAIDHIHEHGSAHTDCVVAEDQEVAEIFLNQVDSAAVIHNASTRFCDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGSGQVVNGDKGVAYTHKDLGVAM
Homology
BLAST of MS019763 vs. NCBI nr
Match: XP_022145741.1 (delta-1-pyrroline-5-carboxylate synthase-like isoform X1 [Momordica charantia])

HSP 1 Score: 1355.9 bits (3508), Expect = 0.0e+00
Identity = 713/741 (96.22%), Postives = 714/741 (96.36%), Query Frame = 0

Query: 1   VHPTRVFVKNVKRLIVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           VHPTRVFVKNVKRLIVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV
Sbjct: 5   VHPTRVFVKNVKRLIVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 64

Query: 61  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV
Sbjct: 65  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 124

Query: 121 TDSVFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TDSVFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL
Sbjct: 125 TDSVFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 184

Query: 181 ALELKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHLGEITFGDKSRLGRGGMTAKV 240
           ALELKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHLGEITFGDKSRLGRGGMTAKV
Sbjct: 185 ALELKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHLGEITFGDKSRLGRGGMTAKV 244

Query: 241 NAAVYAASAGIPVVITRYMIHLVTHFEVFDTSIVTPSLIPNLVSGFATDNIIKVLQGEMK 300
           NAAVYAASAGIPVVIT                           SGFATDNIIKVLQGEMK
Sbjct: 245 NAAVYAASAGIPVVIT---------------------------SGFATDNIIKVLQGEMK 304

Query: 301 GTLFHKDAHLWTVVKEISARDMAVSARESSRRLQALKSEDRRKILLDVADALEDNEDMII 360
           GTLFHKDAHLWTVVKEISARDMAVSARESSR+LQALKSEDRRKILLDVADALEDNEDMII
Sbjct: 305 GTLFHKDAHLWTVVKEISARDMAVSARESSRQLQALKSEDRRKILLDVADALEDNEDMII 364

Query: 361 AENLADIKAAEEVGYEKSLISRLALKPGRIGTLASSIRKLADMEEPIGRILKRKELADDL 420
           AENLADIKAAEEVGYEKSLISRLALKPGRIGTLASSIRKLADMEEPIGRILKRKELADDL
Sbjct: 365 AENLADIKAAEEVGYEKSLISRLALKPGRIGTLASSIRKLADMEEPIGRILKRKELADDL 424

Query: 421 ILENTSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAI 480
           ILENTSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAI
Sbjct: 425 ILENTSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAI 484

Query: 481 PDTVGEKLIGLITSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICH 540
           PDTVGEKLIGLITSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICH
Sbjct: 485 PDTVGEKLIGLITSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICH 544

Query: 541 VYVDKSADIEMAKRIVSDAKADYPAACNAMETLLVHKDLASNGMLIELVTELRKEGVTLY 600
           VYVDKSADIEMAKRIVSDAKADYPAACNAMETLLVHKDLASNGMLIELVTELRKEGVTLY
Sbjct: 545 VYVDKSADIEMAKRIVSDAKADYPAACNAMETLLVHKDLASNGMLIELVTELRKEGVTLY 604

Query: 601 GGKRASAILKIKEASSFHHEYNSLACTVEIVDDVFSAIDHIHEHGSAHTDCVVAEDQEVA 660
           GGKRASAILKIKEASSFHHEYNSLACTVEIVDDVFSAIDHIHEHGSAHTDCVVAEDQEVA
Sbjct: 605 GGKRASAILKIKEASSFHHEYNSLACTVEIVDDVFSAIDHIHEHGSAHTDCVVAEDQEVA 664

Query: 661 EIFLNQVDSAAVIHNASTRFCDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGS 720
           EIFLNQVDSAAVIHNASTRFCDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGS
Sbjct: 665 EIFLNQVDSAAVIHNASTRFCDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGS 718

Query: 721 GQVVNGDKGVAYTHKDLGVAM 742
           GQVVNGDKGVAYTHKDLGVAM
Sbjct: 725 GQVVNGDKGVAYTHKDLGVAM 718

BLAST of MS019763 vs. NCBI nr
Match: XP_022145742.1 (delta-1-pyrroline-5-carboxylate synthase-like isoform X2 [Momordica charantia])

HSP 1 Score: 1343.9 bits (3477), Expect = 0.0e+00
Identity = 705/739 (95.40%), Postives = 710/739 (96.08%), Query Frame = 0

Query: 3   PTRVFVKNVKRLIVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGL 62
           P+RVF +N +RL+VKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGL
Sbjct: 3   PSRVFTRNARRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGL 62

Query: 63  GRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTD 122
           GRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTD
Sbjct: 63  GRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTD 122

Query: 123 SVFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLAL 182
           SVFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLAL
Sbjct: 123 SVFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLAL 182

Query: 183 ELKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHLGEITFGDKSRLGRGGMTAKVNA 242
           ELKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHLGEITFGDKSRLGRGGMTAKVNA
Sbjct: 183 ELKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHLGEITFGDKSRLGRGGMTAKVNA 242

Query: 243 AVYAASAGIPVVITRYMIHLVTHFEVFDTSIVTPSLIPNLVSGFATDNIIKVLQGEMKGT 302
           AVYAASAGIPVVIT                           SGFATDNIIKVLQGEMKGT
Sbjct: 243 AVYAASAGIPVVIT---------------------------SGFATDNIIKVLQGEMKGT 302

Query: 303 LFHKDAHLWTVVKEISARDMAVSARESSRRLQALKSEDRRKILLDVADALEDNEDMIIAE 362
           LFHKDAHLWTVVKEISARDMAVSARESSR+LQALKSEDRRKILLDVADALEDNEDMIIAE
Sbjct: 303 LFHKDAHLWTVVKEISARDMAVSARESSRQLQALKSEDRRKILLDVADALEDNEDMIIAE 362

Query: 363 NLADIKAAEEVGYEKSLISRLALKPGRIGTLASSIRKLADMEEPIGRILKRKELADDLIL 422
           NLADIKAAEEVGYEKSLISRLALKPGRIGTLASSIRKLADMEEPIGRILKRKELADDLIL
Sbjct: 363 NLADIKAAEEVGYEKSLISRLALKPGRIGTLASSIRKLADMEEPIGRILKRKELADDLIL 422

Query: 423 ENTSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPD 482
           ENTSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPD
Sbjct: 423 ENTSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPD 482

Query: 483 TVGEKLIGLITSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVY 542
           TVGEKLIGLITSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVY
Sbjct: 483 TVGEKLIGLITSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVY 542

Query: 543 VDKSADIEMAKRIVSDAKADYPAACNAMETLLVHKDLASNGMLIELVTELRKEGVTLYGG 602
           VDKSADIEMAKRIVSDAKADYPAACNAMETLLVHKDLASNGMLIELVTELRKEGVTLYGG
Sbjct: 543 VDKSADIEMAKRIVSDAKADYPAACNAMETLLVHKDLASNGMLIELVTELRKEGVTLYGG 602

Query: 603 KRASAILKIKEASSFHHEYNSLACTVEIVDDVFSAIDHIHEHGSAHTDCVVAEDQEVAEI 662
           KRASAILKIKEASSFHHEYNSLACTVEIVDDVFSAIDHIHEHGSAHTDCVVAEDQEVAEI
Sbjct: 603 KRASAILKIKEASSFHHEYNSLACTVEIVDDVFSAIDHIHEHGSAHTDCVVAEDQEVAEI 662

Query: 663 FLNQVDSAAVIHNASTRFCDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGSGQ 722
           FLNQVDSAAVIHNASTRFCDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGSGQ
Sbjct: 663 FLNQVDSAAVIHNASTRFCDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGSGQ 714

Query: 723 VVNGDKGVAYTHKDLGVAM 742
           VVNGDKGVAYTHKDLGVAM
Sbjct: 723 VVNGDKGVAYTHKDLGVAM 714

BLAST of MS019763 vs. NCBI nr
Match: XP_038905634.1 (delta-1-pyrroline-5-carboxylate synthase-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1270.8 bits (3287), Expect = 0.0e+00
Identity = 657/737 (89.15%), Postives = 689/737 (93.49%), Query Frame = 0

Query: 1   VHPTRVFVKNVKRLIVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           + PTR+F+K VKRL+VKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV
Sbjct: 4   IDPTRIFIKTVKRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 63

Query: 61  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYR+LANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV
Sbjct: 64  GLGRQRLRYRKLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 123

Query: 121 TDSVFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TDSVF D+GFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL
Sbjct: 124 TDSVFSDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 183

Query: 181 ALELKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHLGEITFGDKSRLGRGGMTAKV 240
           ALELKADLLVLLSDVEGLYSGPP DPNSKLI+TF+KEKHLG+ITFGDKSR+GRGGMTAKV
Sbjct: 184 ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLGKITFGDKSRVGRGGMTAKV 243

Query: 241 NAAVYAASAGIPVVITRYMIHLVTHFEVFDTSIVTPSLIPNLVSGFATDNIIKVLQGEMK 300
           NAAVYA+ AGIPVVIT                           SGFATDNIIKVLQGEMK
Sbjct: 244 NAAVYASCAGIPVVIT---------------------------SGFATDNIIKVLQGEMK 303

Query: 301 GTLFHKDAHLWTVVKEISARDMAVSARESSRRLQALKSEDRRKILLDVADALEDNEDMII 360
           GTLFHKDAHLWT+VKE+SAR+MAVSARESSR+LQALKSEDR+KILLDVADALEDNE+MI+
Sbjct: 304 GTLFHKDAHLWTLVKEVSAREMAVSARESSRQLQALKSEDRKKILLDVADALEDNENMIM 363

Query: 361 AENLADIKAAEEVGYEKSLISRLALKPGRIGTLASSIRKLADMEEPIGRILKRKELADDL 420
           AEN ADIKAAEE GYEK LISRLALKPG+I  LA+SIRKLADMEEPIGRILKR ELA DL
Sbjct: 364 AENNADIKAAEEAGYEKPLISRLALKPGKIKLLANSIRKLADMEEPIGRILKRSELASDL 423

Query: 421 ILENTSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAI 480
           +LEN SCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAI
Sbjct: 424 VLENVSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAI 483

Query: 481 PDTVGEKLIGLITSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICH 540
           PDTVGEKLIGL+TSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICH
Sbjct: 484 PDTVGEKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICH 543

Query: 541 VYVDKSADIEMAKRIVSDAKADYPAACNAMETLLVHKDLASNGMLIELVTELRKEGVTLY 600
           VYVDKSAD+EMAKRIVSD+K DYPAACNAMETLLVHKDLASNG LI+LVTELRKEGVTLY
Sbjct: 544 VYVDKSADMEMAKRIVSDSKVDYPAACNAMETLLVHKDLASNGGLIKLVTELRKEGVTLY 603

Query: 601 GGKRASAILKIKEASSFHHEYNSLACTVEIVDDVFSAIDHIHEHGSAHTDCVVAEDQEVA 660
           GGKRAS +LKIKEASSFHHEYNSL CTVEIVDDVF+AIDHIH+HGS+HTDC++ EDQEVA
Sbjct: 604 GGKRASELLKIKEASSFHHEYNSLTCTVEIVDDVFAAIDHIHQHGSSHTDCIITEDQEVA 663

Query: 661 EIFLNQVDSAAVIHNASTRFCDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGS 720
           EIFLNQVDSAAV HNASTRFCDGARFGLGAEVGIST RIHARGPVGVEGLLTTRWILRGS
Sbjct: 664 EIFLNQVDSAAVFHNASTRFCDGARFGLGAEVGISTSRIHARGPVGVEGLLTTRWILRGS 713

Query: 721 GQVVNGDKGVAYTHKDL 738
           GQ+VNGDKGVAY HKDL
Sbjct: 724 GQIVNGDKGVAYIHKDL 713

BLAST of MS019763 vs. NCBI nr
Match: XP_038905635.1 (delta-1-pyrroline-5-carboxylate synthase-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1268.8 bits (3282), Expect = 0.0e+00
Identity = 657/735 (89.39%), Postives = 688/735 (93.61%), Query Frame = 0

Query: 3   PTRVFVKNVKRLIVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGL 62
           P+RVF KNV+RL+VKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGL
Sbjct: 3   PSRVFTKNVRRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGL 62

Query: 63  GRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTD 122
           GRQRLRYR+LANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTD
Sbjct: 63  GRQRLRYRKLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTD 122

Query: 123 SVFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLAL 182
           SVF D+GFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLAL
Sbjct: 123 SVFSDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLAL 182

Query: 183 ELKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHLGEITFGDKSRLGRGGMTAKVNA 242
           ELKADLLVLLSDVEGLYSGPP DPNSKLI+TF+KEKHLG+ITFGDKSR+GRGGMTAKVNA
Sbjct: 183 ELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLGKITFGDKSRVGRGGMTAKVNA 242

Query: 243 AVYAASAGIPVVITRYMIHLVTHFEVFDTSIVTPSLIPNLVSGFATDNIIKVLQGEMKGT 302
           AVYA+ AGIPVVIT                           SGFATDNIIKVLQGEMKGT
Sbjct: 243 AVYASCAGIPVVIT---------------------------SGFATDNIIKVLQGEMKGT 302

Query: 303 LFHKDAHLWTVVKEISARDMAVSARESSRRLQALKSEDRRKILLDVADALEDNEDMIIAE 362
           LFHKDAHLWT+VKE+SAR+MAVSARESSR+LQALKSEDR+KILLDVADALEDNE+MI+AE
Sbjct: 303 LFHKDAHLWTLVKEVSAREMAVSARESSRQLQALKSEDRKKILLDVADALEDNENMIMAE 362

Query: 363 NLADIKAAEEVGYEKSLISRLALKPGRIGTLASSIRKLADMEEPIGRILKRKELADDLIL 422
           N ADIKAAEE GYEK LISRLALKPG+I  LA+SIRKLADMEEPIGRILKR ELA DL+L
Sbjct: 363 NNADIKAAEEAGYEKPLISRLALKPGKIKLLANSIRKLADMEEPIGRILKRSELASDLVL 422

Query: 423 ENTSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPD 482
           EN SCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPD
Sbjct: 423 ENVSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPD 482

Query: 483 TVGEKLIGLITSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVY 542
           TVGEKLIGL+TSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVY
Sbjct: 483 TVGEKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVY 542

Query: 543 VDKSADIEMAKRIVSDAKADYPAACNAMETLLVHKDLASNGMLIELVTELRKEGVTLYGG 602
           VDKSAD+EMAKRIVSD+K DYPAACNAMETLLVHKDLASNG LI+LVTELRKEGVTLYGG
Sbjct: 543 VDKSADMEMAKRIVSDSKVDYPAACNAMETLLVHKDLASNGGLIKLVTELRKEGVTLYGG 602

Query: 603 KRASAILKIKEASSFHHEYNSLACTVEIVDDVFSAIDHIHEHGSAHTDCVVAEDQEVAEI 662
           KRAS +LKIKEASSFHHEYNSL CTVEIVDDVF+AIDHIH+HGS+HTDC++ EDQEVAEI
Sbjct: 603 KRASELLKIKEASSFHHEYNSLTCTVEIVDDVFAAIDHIHQHGSSHTDCIITEDQEVAEI 662

Query: 663 FLNQVDSAAVIHNASTRFCDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGSGQ 722
           FLNQVDSAAV HNASTRFCDGARFGLGAEVGIST RIHARGPVGVEGLLTTRWILRGSGQ
Sbjct: 663 FLNQVDSAAVFHNASTRFCDGARFGLGAEVGISTSRIHARGPVGVEGLLTTRWILRGSGQ 710

Query: 723 VVNGDKGVAYTHKDL 738
           +VNGDKGVAY HKDL
Sbjct: 723 IVNGDKGVAYIHKDL 710

BLAST of MS019763 vs. NCBI nr
Match: XP_023529105.1 (delta-1-pyrroline-5-carboxylate synthase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1258.4 bits (3255), Expect = 0.0e+00
Identity = 648/735 (88.16%), Postives = 685/735 (93.20%), Query Frame = 0

Query: 3   PTRVFVKNVKRLIVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGL 62
           PTR F++N+KRL+VKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGL
Sbjct: 6   PTRTFIRNIKRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGL 65

Query: 63  GRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTD 122
           GRQRLRYR+LANSSFADLQNPQG+FDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTD
Sbjct: 66  GRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTD 125

Query: 123 SVFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLAL 182
           SVFCD+GFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLAL
Sbjct: 126 SVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLAL 185

Query: 183 ELKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHLGEITFGDKSRLGRGGMTAKVNA 242
           ELKADLLVLLSDVEGLYSGPP DP SKLI+TF+KEKHLGEITFGDKSR+GRGGMTAKVNA
Sbjct: 186 ELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKVNA 245

Query: 243 AVYAASAGIPVVITRYMIHLVTHFEVFDTSIVTPSLIPNLVSGFATDNIIKVLQGEMKGT 302
           AV A+ AGIPVVIT                           SGFATDNIIKVLQGEMKGT
Sbjct: 246 AVCASCAGIPVVIT---------------------------SGFATDNIIKVLQGEMKGT 305

Query: 303 LFHKDAHLWTVVKEISARDMAVSARESSRRLQALKSEDRRKILLDVADALEDNEDMIIAE 362
           LFHKDAHLWT+VKE+SAR+MAV ARESSRRLQALKSEDRRKILLDVA+AL+DNEDMI+AE
Sbjct: 306 LFHKDAHLWTLVKEVSAREMAVEARESSRRLQALKSEDRRKILLDVANALQDNEDMIMAE 365

Query: 363 NLADIKAAEEVGYEKSLISRLALKPGRIGTLASSIRKLADMEEPIGRILKRKELADDLIL 422
           N ADIKAAEE GYEKSLISRLALKPGRI  LA+SIRK+ADMEEPIGRILKRKE+A DLIL
Sbjct: 366 NNADIKAAEEAGYEKSLISRLALKPGRIELLANSIRKIADMEEPIGRILKRKEIASDLIL 425

Query: 423 ENTSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPD 482
           ENT+CSLGVLL+VFESRPDALVQIAALAIRSGNGLLLKGGKEAR+SNAALHKVITSAIPD
Sbjct: 426 ENTTCSLGVLLVVFESRPDALVQIAALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPD 485

Query: 483 TVGEKLIGLITSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVY 542
           TVG KLIGL+TSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVY
Sbjct: 486 TVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVY 545

Query: 543 VDKSADIEMAKRIVSDAKADYPAACNAMETLLVHKDLASNGMLIELVTELRKEGVTLYGG 602
           VDKSADIEMAKRIVSD+K DYPAACNAMETLLVHKDLASNG L+ELV ELRKEGVTLYGG
Sbjct: 546 VDKSADIEMAKRIVSDSKVDYPAACNAMETLLVHKDLASNGGLMELVAELRKEGVTLYGG 605

Query: 603 KRASAILKIKEASSFHHEYNSLACTVEIVDDVFSAIDHIHEHGSAHTDCVVAEDQEVAEI 662
           +RAS +L+I EASSFHHEYNSL CTVEIVDDVF+AIDHIH+HGS+HTDC++ +DQEVAEI
Sbjct: 606 QRASQLLRISEASSFHHEYNSLTCTVEIVDDVFAAIDHIHQHGSSHTDCIITKDQEVAEI 665

Query: 663 FLNQVDSAAVIHNASTRFCDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGSGQ 722
           FLNQVDSAAV HNASTRFCDGARFGLGAEVG+ST RIHARGPVGVEGLLTTRWILRG+GQ
Sbjct: 666 FLNQVDSAAVFHNASTRFCDGARFGLGAEVGVSTSRIHARGPVGVEGLLTTRWILRGNGQ 713

Query: 723 VVNGDKGVAYTHKDL 738
           VVNGDKGV Y HKDL
Sbjct: 726 VVNGDKGVTYIHKDL 713

BLAST of MS019763 vs. ExPASy Swiss-Prot
Match: O65361 (Delta-1-pyrroline-5-carboxylate synthase OS=Mesembryanthemum crystallinum OX=3544 GN=P5CS PE=2 SV=1)

HSP 1 Score: 1080.5 bits (2793), Expect = 0.0e+00
Identity = 556/736 (75.54%), Postives = 628/736 (85.33%), Query Frame = 0

Query: 4   TRVFVKNVKRLIVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGLG 63
           TR FVK+VKR++VKVGTAVVTR DGRLALGRLG+LCEQLKELNS GYEVILVTSGAV  G
Sbjct: 4   TRAFVKDVKRVVVKVGTAVVTRSDGRLALGRLGSLCEQLKELNSDGYEVILVTSGAVSAG 63

Query: 64  RQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTDS 123
           RQRLR+R+L NSSFADLQ PQ + DGKACAAVGQ+ LMALYDT+FSQLD+T++QLLVTD+
Sbjct: 64  RQRLRFRKLVNSSFADLQKPQVELDGKACAAVGQNGLMALYDTLFSQLDLTAAQLLVTDN 123

Query: 124 VFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLALE 183
            F D  FR QL+ETV  LLDL+V+P+ NENDAVSTRK PYEDSSGIFWDNDSLA LLALE
Sbjct: 124 DFRDPSFRTQLTETVYQLLDLKVVPVLNENDAVSTRKAPYEDSSGIFWDNDSLAALLALE 183

Query: 184 LKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHLGEITFGDKSRLGRGGMTAKVNAA 243
           LKADLL+LLSDV+GLY+GPP DP SKLI T++KEKH GEITFGDKSRLGRGGMTAKV AA
Sbjct: 184 LKADLLILLSDVDGLYNGPPSDPRSKLISTYVKEKHQGEITFGDKSRLGRGGMTAKVKAA 243

Query: 244 VYAASAGIPVVITRYMIHLVTHFEVFDTSIVTPSLIPNLVSGFATDNIIKVLQGEMKGTL 303
           VYAA AGIPV+I                            SG ATDNIIKV+ G+  GTL
Sbjct: 244 VYAAYAGIPVII---------------------------ASGKATDNIIKVIDGQCVGTL 303

Query: 304 FHKDAHLWTVVKEISARDMAVSARESSRRLQALKSEDRRKILLDVADALEDNEDMIIAEN 363
           FHKDAHLW  VKE   RDMAV+ARESSRRLQA+ SE+R+KILLD+ADALE NE+ I+AEN
Sbjct: 304 FHKDAHLWVQVKETGVRDMAVAARESSRRLQAVSSEERKKILLDIADALEANEEKILAEN 363

Query: 364 LADIKAAEEVGYEKSLISRLALKPGRIGTLASSIRKLADMEEPIGRILKRKELADDLILE 423
            AD+ AA+  GY++SL++RLA+ P +I +LA SIR LADMEEPIGRILKR E+AD LILE
Sbjct: 364 EADVAAAQYAGYDRSLVARLAMNPDKISSLAKSIRVLADMEEPIGRILKRTEIADGLILE 423

Query: 424 NTSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDT 483
            TSC LGVLLIVFESRPDALVQIA+LAIRSGNGLLLKGGKEA+RSNA LHKVITSAIPD 
Sbjct: 424 KTSCPLGVLLIVFESRPDALVQIASLAIRSGNGLLLKGGKEAKRSNAILHKVITSAIPDK 483

Query: 484 VGEKLIGLITSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYV 543
           VGEKLIGL+TSR+EIPDLLKLDDVIDLVIPRGSNKLVSQIKEST+IPVLGH+DGICHVYV
Sbjct: 484 VGEKLIGLVTSRDEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTRIPVLGHADGICHVYV 543

Query: 544 DKSADIEMAKRIVSDAKADYPAACNAMETLLVHKDLASNGMLIELVTELRKEGVTLYGGK 603
           DKSA+++MAKRIV DAK DYPAACNAMETLLVHKDLA NG L +L+ +LR EGVT++GG 
Sbjct: 544 DKSANMDMAKRIVLDAKTDYPAACNAMETLLVHKDLAENGGLNDLIVDLRTEGVTMFGGP 603

Query: 604 RASAI--LKIKEASSFHHEYNSLACTVEIVDDVFSAIDHIHEHGSAHTDCVVAEDQEVAE 663
           R  A+    I+   +F+ EY+S ACTVEIVDDV++AI+HI+ HGSAHTDC++AED +VAE
Sbjct: 604 RIDALQEFNIQATQTFNREYSSPACTVEIVDDVYAAIEHINHHGSAHTDCIIAEDHKVAE 663

Query: 664 IFLNQVDSAAVIHNASTRFCDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGSG 723
            FL  VDSAAV+HNASTRFCDG RFGLGAEVGIST RIHARGPVGVEGLLTTRW+L+GSG
Sbjct: 664 TFLQLVDSAAVLHNASTRFCDGFRFGLGAEVGISTSRIHARGPVGVEGLLTTRWVLKGSG 712

Query: 724 QVVNGDKGVAYTHKDL 738
           QVV+GDKGV YTHKDL
Sbjct: 724 QVVHGDKGVVYTHKDL 712

BLAST of MS019763 vs. ExPASy Swiss-Prot
Match: O04015 (Delta-1-pyrroline-5-carboxylate synthase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 1078.9 bits (2789), Expect = 0.0e+00
Identity = 559/737 (75.85%), Postives = 628/737 (85.21%), Query Frame = 0

Query: 1   VHPTRVFVKNVKRLIVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           V  TR FVK VKR+I+KVGTAVVTR DGRLALGRLGALCEQ+ ELNS+G+EVILVTSGAV
Sbjct: 4   VDSTRAFVKGVKRVIIKVGTAVVTRADGRLALGRLGALCEQIHELNSQGFEVILVTSGAV 63

Query: 61  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           G+GRQRLRYR+L NSSFADLQ PQ + DGKACAAVGQ+ L+ALYDT+FSQLDVTS+QLLV
Sbjct: 64  GVGRQRLRYRKLVNSSFADLQKPQIELDGKACAAVGQNGLLALYDTLFSQLDVTSAQLLV 123

Query: 121 TDSVFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TD+ F D  FR+QL+ETV SLL+L+VIPIFNENDAVSTRK PYED+SGIFWDNDSLA LL
Sbjct: 124 TDNDFRDPEFRKQLTETVESLLNLKVIPIFNENDAVSTRKAPYEDASGIFWDNDSLAALL 183

Query: 181 ALELKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHLGEITFGDKSRLGRGGMTAKV 240
           ALELKADLLVLLSDVEGLYSGPP DP SKLI T+IKE   G ITFGDKSR+GRGGMTAKV
Sbjct: 184 ALELKADLLVLLSDVEGLYSGPPSDPQSKLIHTYIKEMFEGLITFGDKSRVGRGGMTAKV 243

Query: 241 NAAVYAASAGIPVVITRYMIHLVTHFEVFDTSIVTPSLIPNLVSGFATDNIIKVLQGEMK 300
            AAVYAA AGIPVVIT                           SG+AT+NIIKVLQGE  
Sbjct: 244 KAAVYAAHAGIPVVIT---------------------------SGYATNNIIKVLQGERI 303

Query: 301 GTLFHKDAHLWTVVKEISARDMAVSARESSRRLQALKSEDRRKILLDVADALEDNEDMII 360
           GTLFH+DA  W  V ++ ARDMAV+ARESSRRLQA+  +DR KILLDVADALE NE +I 
Sbjct: 304 GTLFHRDAQKWAPVGDVGARDMAVAARESSRRLQAMSPQDRSKILLDVADALEANEKLIR 363

Query: 361 AENLADIKAAEEVGYEKSLISRLALKPGRIGTLASSIRKLADMEEPIGRILKRKELADDL 420
            EN AD+ AA++ GYEKSLISRLALK G+I +LA SIR LA+MEEPIG +LKR E+ D L
Sbjct: 364 IENEADLAAAQQAGYEKSLISRLALKSGKISSLAKSIRVLANMEEPIGHVLKRTEITDGL 423

Query: 421 ILENTSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAI 480
           +LE TS  LGVLLI+FESRPDALVQIA+LAIRSGNGL+LKGGKEA+RSNA LHKVITSAI
Sbjct: 424 VLEKTSSPLGVLLIIFESRPDALVQIASLAIRSGNGLVLKGGKEAKRSNAILHKVITSAI 483

Query: 481 PDTVGEKLIGLITSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICH 540
           P+ VG +LIGL+TSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGH+DGICH
Sbjct: 484 PENVGPRLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHADGICH 543

Query: 541 VYVDKSADIEMAKRIVSDAKADYPAACNAMETLLVHKDLASNGMLIELVTELRKEGVTLY 600
           VYVDKSA+++MAK++V DAK DYPAACNAMETLLVHKDL  NG L EL+ EL+ +GV ++
Sbjct: 544 VYVDKSANMDMAKKVVLDAKTDYPAACNAMETLLVHKDLVQNGCLDELIVELQIKGVVIH 603

Query: 601 GGKRASAILKIKEASSFHHEYNSLACTVEIVDDVFSAIDHIHEHGSAHTDCVVAEDQEVA 660
           GG RAS++L I EA S HHEY+SLACT+EIVDDV++AIDHIH HGSAHTD ++ ED EVA
Sbjct: 604 GGPRASSLLHIPEARSLHHEYSSLACTIEIVDDVYAAIDHIHRHGSAHTDSIITEDHEVA 663

Query: 661 EIFLNQVDSAAVIHNASTRFCDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGS 720
           EIFL QVDS++V+HNASTRF DGARFGLGAEVGIST RIHARGPVGVEGLLTTRWI RGS
Sbjct: 664 EIFLRQVDSSSVLHNASTRFSDGARFGLGAEVGISTSRIHARGPVGVEGLLTTRWIARGS 713

Query: 721 GQVVNGDKGVAYTHKDL 738
           GQVV+GDKG+ YTHKDL
Sbjct: 724 GQVVDGDKGIVYTHKDL 713

BLAST of MS019763 vs. ExPASy Swiss-Prot
Match: Q96480 (Delta-1-pyrroline-5-carboxylate synthase OS=Solanum lycopersicum OX=4081 GN=PRO2 PE=2 SV=1)

HSP 1 Score: 1064.3 bits (2751), Expect = 6.2e-310
Identity = 549/737 (74.49%), Postives = 628/737 (85.21%), Query Frame = 0

Query: 1   VHPTRVFVKNVKRLIVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           V  TR FVKNVKRLIVKVGTAVVTR DGRLALGRLGALCEQL+ELNS+GYEVILVTSGAV
Sbjct: 4   VDSTRAFVKNVKRLIVKVGTAVVTRADGRLALGRLGALCEQLQELNSQGYEVILVTSGAV 63

Query: 61  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           G+GRQRLRYR+L NSSF DLQ PQ + DGKACAAVGQ+ LMALYD++FSQLDVTS+QLLV
Sbjct: 64  GVGRQRLRYRKLLNSSFLDLQKPQTELDGKACAAVGQNGLMALYDSLFSQLDVTSAQLLV 123

Query: 121 TDSVFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TD+ F D  FR+QL++TVNSLL L+VIPIFNENDA+STR+ PYEDSSGIFWDNDSLA LL
Sbjct: 124 TDNDFRDPDFRRQLNDTVNSLLSLKVIPIFNENDAISTRRAPYEDSSGIFWDNDSLAALL 183

Query: 181 ALELKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHLGEITFGDKSRLGRGGMTAKV 240
           ALELKADLLVLLSDV+GLYSGPP DP+SKLI T+IKE H   ITFGDKSR+GRGGMTAKV
Sbjct: 184 ALELKADLLVLLSDVDGLYSGPPRDPDSKLIYTYIKEIHERVITFGDKSRVGRGGMTAKV 243

Query: 241 NAAVYAASAGIPVVITRYMIHLVTHFEVFDTSIVTPSLIPNLVSGFATDNIIKVLQGEMK 300
            AA+YAA AGIPVVIT                           SGFATDNIIKVL GE  
Sbjct: 244 KAAMYAAYAGIPVVIT---------------------------SGFATDNIIKVLHGERI 303

Query: 301 GTLFHKDAHLWTVVKEISARDMAVSARESSRRLQALKSEDRRKILLDVADALEDNEDMII 360
           GTLFH DA+ W  + E  AR+MAV+AR  SRRLQAL S++R KIL D+ADALE NE  I+
Sbjct: 304 GTLFHCDANKWASIGETDAREMAVAARACSRRLQALSSQERSKILQDIADALEANEKAIL 363

Query: 361 AENLADIKAAEEVGYEKSLISRLALKPGRIGTLASSIRKLADMEEPIGRILKRKELADDL 420
           AEN AD+ AA++ GYEKSLISRLAL PG+I +LA+S+R L++M+EP+G  LKR E+AD  
Sbjct: 364 AENEADVVAAQQAGYEKSLISRLALNPGKISSLANSVRVLSNMDEPLGHTLKRTEIADGF 423

Query: 421 ILENTSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAI 480
           ILE +S  LGV+LI+FESRPDALVQIA+LA+RSGNGL+LKGGKEA+RSNA LHKVITSAI
Sbjct: 424 ILEKSSSPLGVVLIIFESRPDALVQIASLAVRSGNGLMLKGGKEAKRSNAILHKVITSAI 483

Query: 481 PDTVGEKLIGLITSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICH 540
           P +VGE+LIGL+TSREEIP+LLKLDDVIDLVIPRGSNKLVSQIK STKIPVLGH+DGICH
Sbjct: 484 PVSVGERLIGLVTSREEIPELLKLDDVIDLVIPRGSNKLVSQIKASTKIPVLGHADGICH 543

Query: 541 VYVDKSADIEMAKRIVSDAKADYPAACNAMETLLVHKDLASNGMLIELVTELRKEGVTLY 600
           VYVDKSAD++MAKRI  DAK DYPAACNAMETLLVHKDLA NG L +L+ EL+ +GV+LY
Sbjct: 544 VYVDKSADMDMAKRITVDAKIDYPAACNAMETLLVHKDLAQNGGLNDLIVELQTKGVSLY 603

Query: 601 GGKRASAILKIKEASSFHHEYNSLACTVEIVDDVFSAIDHIHEHGSAHTDCVVAEDQEVA 660
           GG +AS++L I EA +F HEY+SLACTVE+V+DV++AIDHIH+HGSAHTD ++ EDQEVA
Sbjct: 604 GGPKASSLLMIPEARTFRHEYSSLACTVEVVEDVYAAIDHIHQHGSAHTDSIITEDQEVA 663

Query: 661 EIFLNQVDSAAVIHNASTRFCDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGS 720
           E+FL QVDSAAV HNASTRF DG RFGLGAEVGISTGRIHARGPVGVEGLLTT+W+ RGS
Sbjct: 664 EVFLRQVDSAAVFHNASTRFSDGFRFGLGAEVGISTGRIHARGPVGVEGLLTTKWLARGS 713

Query: 721 GQVVNGDKGVAYTHKDL 738
           GQ+V+GDK + Y+HKDL
Sbjct: 724 GQIVDGDKSIVYSHKDL 713

BLAST of MS019763 vs. ExPASy Swiss-Prot
Match: P54887 (Delta-1-pyrroline-5-carboxylate synthase A OS=Arabidopsis thaliana OX=3702 GN=P5CSA PE=1 SV=1)

HSP 1 Score: 1053.9 bits (2724), Expect = 8.4e-307
Identity = 541/736 (73.51%), Postives = 617/736 (83.83%), Query Frame = 0

Query: 4   TRVFVKNVKRLIVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGLG 63
           +R F ++VKR++VKVGTAVVT   GRLALGRLGALCEQL ELNS G+EVILV+SGAVGLG
Sbjct: 7   SRAFARDVKRIVVKVGTAVVTGKGGRLALGRLGALCEQLAELNSDGFEVILVSSGAVGLG 66

Query: 64  RQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTDS 123
           RQRLRYR+L NSSFADLQ PQ + DGKACA VGQSSLMA Y+TMF QLDVT++QLLV DS
Sbjct: 67  RQRLRYRQLVNSSFADLQKPQTELDGKACAGVGQSSLMAYYETMFDQLDVTAAQLLVNDS 126

Query: 124 VFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLALE 183
            F D  FR+QL+ETV S+LDLRVIPIFNENDA+STR+ PY+DSSGIFWDNDSLA LLALE
Sbjct: 127 SFRDKDFRKQLNETVKSMLDLRVIPIFNENDAISTRRAPYQDSSGIFWDNDSLAALLALE 186

Query: 184 LKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHLGEITFGDKSRLGRGGMTAKVNAA 243
           LKADLL+LLSDVEGLY+GPP DPNSKLI TF+KEKH  EITFGDKSRLGRGGMTAKV AA
Sbjct: 187 LKADLLILLSDVEGLYTGPPSDPNSKLIHTFVKEKHQDEITFGDKSRLGRGGMTAKVKAA 246

Query: 244 VYAASAGIPVVITRYMIHLVTHFEVFDTSIVTPSLIPNLVSGFATDNIIKVLQGEMKGTL 303
           V AA AGIPV+IT                           SG++ +NI KVL+G   GTL
Sbjct: 247 VNAAYAGIPVIIT---------------------------SGYSAENIDKVLRGLRVGTL 306

Query: 304 FHKDAHLWTVVKEISARDMAVSARESSRRLQALKSEDRRKILLDVADALEDNEDMIIAEN 363
           FH+DA LW  + + +ARDMAV+ARESSR+LQAL SEDR+KILLD+ADALE N   I AEN
Sbjct: 307 FHQDARLWAPITDSNARDMAVAARESSRKLQALSSEDRKKILLDIADALEANVTTIKAEN 366

Query: 364 LADIKAAEEVGYEKSLISRLALKPGRIGTLASSIRKLADMEEPIGRILKRKELADDLILE 423
             D+ +A+E G E+S+++RL + PG+I +LA+S+RKLADME+PIGR+LK+ E+AD L+LE
Sbjct: 367 ELDVASAQEAGLEESMVARLVMTPGKISSLAASVRKLADMEDPIGRVLKKTEVADGLVLE 426

Query: 424 NTSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDT 483
            TS  LGVLLIVFESRPDALVQIA+LAIRSGNGLLLKGGKEARRSNA LHKVIT AIP+T
Sbjct: 427 KTSSPLGVLLIVFESRPDALVQIASLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPET 486

Query: 484 VGEKLIGLITSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYV 543
           VG KLIGL+TSREEIPDLLKLDDVIDLVIPRGSNKLV+QIK +TKIPVLGH+DGICHVYV
Sbjct: 487 VGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVTQIKNTTKIPVLGHADGICHVYV 546

Query: 544 DKSADIEMAKRIVSDAKADYPAACNAMETLLVHKDLASNGMLIELVTELRKEGVTLYGGK 603
           DK+ D +MAKRIVSDAK DYPAACNAMETLLVHKDL  N +L EL+  L+  GVTLYGG 
Sbjct: 547 DKACDTDMAKRIVSDAKLDYPAACNAMETLLVHKDLEQNAVLNELIFALQSNGVTLYGGP 606

Query: 604 RASAILKIKEASSFHHEYNSLACTVEIVDDVFSAIDHIHEHGSAHTDCVVAEDQEVAEIF 663
           RAS IL I EA SF+HEY + ACTVE+V+DV+ AIDHIH HGSAHTDC+V ED EVAE+F
Sbjct: 607 RASKILNIPEARSFNHEYCAKACTVEVVEDVYGAIDHIHRHGSAHTDCIVTEDHEVAELF 666

Query: 664 LNQVDSAAVIHNASTRFCDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGSGQV 723
           L QVDSAAV HNASTRF DG RFGLGAEVG+STGRIHARGPVGVEGLLTTRWI+RG GQV
Sbjct: 667 LRQVDSAAVFHNASTRFSDGFRFGLGAEVGVSTGRIHARGPVGVEGLLTTRWIMRGKGQV 715

Query: 724 VNGDKGVAYTHKDLGV 740
           V+GD G+ YTH+D+ +
Sbjct: 727 VDGDNGIVYTHQDIPI 715

BLAST of MS019763 vs. ExPASy Swiss-Prot
Match: P54888 (Delta-1-pyrroline-5-carboxylate synthase B OS=Arabidopsis thaliana OX=3702 GN=P5CSB PE=2 SV=1)

HSP 1 Score: 1050.8 bits (2716), Expect = 7.1e-306
Identity = 541/736 (73.51%), Postives = 618/736 (83.97%), Query Frame = 0

Query: 4   TRVFVKNVKRLIVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGLG 63
           +R F K+VKR++VKVGTAVVT   GRLALGRLGA+CEQL ELNS G+EVILV+SGAVGLG
Sbjct: 7   SRAFAKDVKRIVVKVGTAVVTGKGGRLALGRLGAICEQLAELNSDGFEVILVSSGAVGLG 66

Query: 64  RQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTDS 123
           RQRLRYR+L NSSFADLQ PQ + DGKACA VGQSSLMA Y+TMF QLDVT +Q+LVTDS
Sbjct: 67  RQRLRYRQLVNSSFADLQKPQMELDGKACAGVGQSSLMAYYETMFDQLDVTVAQMLVTDS 126

Query: 124 VFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLALE 183
            F D  FR+QLSETV ++L +RVIP+FNENDA+STR+ PY+DS+GIFWDNDSLA LL+LE
Sbjct: 127 SFRDKDFRKQLSETVKAMLRMRVIPVFNENDAISTRRAPYKDSTGIFWDNDSLAALLSLE 186

Query: 184 LKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHLGEITFGDKSRLGRGGMTAKVNAA 243
           LKADLL+LLSDVEGLY+GPP D  SKLI TFIKEKH  EITFG+KS+LGRGGMTAKV AA
Sbjct: 187 LKADLLILLSDVEGLYTGPPSDSTSKLIHTFIKEKHQDEITFGEKSKLGRGGMTAKVKAA 246

Query: 244 VYAASAGIPVVITRYMIHLVTHFEVFDTSIVTPSLIPNLVSGFATDNIIKVLQGEMKGTL 303
           V AA  G+PV+IT                           SG+A +NI KVL+G   GTL
Sbjct: 247 VNAAYGGVPVIIT---------------------------SGYAAENISKVLRGLRVGTL 306

Query: 304 FHKDAHLWTVVKEISARDMAVSARESSRRLQALKSEDRRKILLDVADALEDNEDMIIAEN 363
           FH+DAHLW  V + ++RDMAV+ARESSR+LQAL SEDR++IL D+A+ALE NE  I AEN
Sbjct: 307 FHQDAHLWAPVVDTTSRDMAVAARESSRKLQALSSEDRKQILHDIANALEVNEKTIKAEN 366

Query: 364 LADIKAAEEVGYEKSLISRLALKPGRIGTLASSIRKLADMEEPIGRILKRKELADDLILE 423
             D+ AA+E GYE+SL++RL +KPG+I +LA+S+R+LA+ME+PIGR+LK+ ++ADDLILE
Sbjct: 367 DLDVAAAQEAGYEESLVARLVMKPGKISSLAASVRQLAEMEDPIGRVLKKTQVADDLILE 426

Query: 424 NTSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDT 483
            TS  +GVLLIVFESRPDALVQIA+LAIRSGNGLLLKGGKEARRSNA LHKVIT AIP+T
Sbjct: 427 KTSSPIGVLLIVFESRPDALVQIASLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPET 486

Query: 484 VGEKLIGLITSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYV 543
           VG KLIGL+TSREEIPDLLKLDDVIDLVIPRGSNKLVSQIK STKIPVLGH+DGICHVYV
Sbjct: 487 VGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKNSTKIPVLGHADGICHVYV 546

Query: 544 DKSADIEMAKRIVSDAKADYPAACNAMETLLVHKDLASNGMLIELVTELRKEGVTLYGGK 603
           DKS  ++MAKRIVSDAK DYPAACNAMETLLVHKDL  NG L +L+  L+ +GVTLYGG 
Sbjct: 547 DKSGKLDMAKRIVSDAKLDYPAACNAMETLLVHKDLEQNGFLDDLIYVLQTKGVTLYGGP 606

Query: 604 RASAILKIKEASSFHHEYNSLACTVEIVDDVFSAIDHIHEHGSAHTDCVVAEDQEVAEIF 663
           RASA L I E  SFHHEY+S ACTVEIV+DV+ AIDHIH+HGSAHTDC+V ED EVAEIF
Sbjct: 607 RASAKLNIPETKSFHHEYSSKACTVEIVEDVYGAIDHIHQHGSAHTDCIVTEDSEVAEIF 666

Query: 664 LNQVDSAAVIHNASTRFCDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGSGQV 723
           L QVDSAAV HNASTRF DG RFGLGAEVGIST RIHARGPVGVEGLLTTRWI+RG GQV
Sbjct: 667 LRQVDSAAVFHNASTRFSDGFRFGLGAEVGISTSRIHARGPVGVEGLLTTRWIMRGKGQV 715

Query: 724 VNGDKGVAYTHKDLGV 740
           V+GD G+ YTHKDL V
Sbjct: 727 VDGDNGIVYTHKDLPV 715

BLAST of MS019763 vs. ExPASy TrEMBL
Match: A0A6J1CX79 (Delta-1-pyrroline-5-carboxylate synthase OS=Momordica charantia OX=3673 GN=LOC111015124 PE=3 SV=1)

HSP 1 Score: 1355.9 bits (3508), Expect = 0.0e+00
Identity = 713/741 (96.22%), Postives = 714/741 (96.36%), Query Frame = 0

Query: 1   VHPTRVFVKNVKRLIVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           VHPTRVFVKNVKRLIVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV
Sbjct: 5   VHPTRVFVKNVKRLIVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 64

Query: 61  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV
Sbjct: 65  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 124

Query: 121 TDSVFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TDSVFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL
Sbjct: 125 TDSVFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 184

Query: 181 ALELKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHLGEITFGDKSRLGRGGMTAKV 240
           ALELKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHLGEITFGDKSRLGRGGMTAKV
Sbjct: 185 ALELKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHLGEITFGDKSRLGRGGMTAKV 244

Query: 241 NAAVYAASAGIPVVITRYMIHLVTHFEVFDTSIVTPSLIPNLVSGFATDNIIKVLQGEMK 300
           NAAVYAASAGIPVVIT                           SGFATDNIIKVLQGEMK
Sbjct: 245 NAAVYAASAGIPVVIT---------------------------SGFATDNIIKVLQGEMK 304

Query: 301 GTLFHKDAHLWTVVKEISARDMAVSARESSRRLQALKSEDRRKILLDVADALEDNEDMII 360
           GTLFHKDAHLWTVVKEISARDMAVSARESSR+LQALKSEDRRKILLDVADALEDNEDMII
Sbjct: 305 GTLFHKDAHLWTVVKEISARDMAVSARESSRQLQALKSEDRRKILLDVADALEDNEDMII 364

Query: 361 AENLADIKAAEEVGYEKSLISRLALKPGRIGTLASSIRKLADMEEPIGRILKRKELADDL 420
           AENLADIKAAEEVGYEKSLISRLALKPGRIGTLASSIRKLADMEEPIGRILKRKELADDL
Sbjct: 365 AENLADIKAAEEVGYEKSLISRLALKPGRIGTLASSIRKLADMEEPIGRILKRKELADDL 424

Query: 421 ILENTSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAI 480
           ILENTSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAI
Sbjct: 425 ILENTSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAI 484

Query: 481 PDTVGEKLIGLITSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICH 540
           PDTVGEKLIGLITSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICH
Sbjct: 485 PDTVGEKLIGLITSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICH 544

Query: 541 VYVDKSADIEMAKRIVSDAKADYPAACNAMETLLVHKDLASNGMLIELVTELRKEGVTLY 600
           VYVDKSADIEMAKRIVSDAKADYPAACNAMETLLVHKDLASNGMLIELVTELRKEGVTLY
Sbjct: 545 VYVDKSADIEMAKRIVSDAKADYPAACNAMETLLVHKDLASNGMLIELVTELRKEGVTLY 604

Query: 601 GGKRASAILKIKEASSFHHEYNSLACTVEIVDDVFSAIDHIHEHGSAHTDCVVAEDQEVA 660
           GGKRASAILKIKEASSFHHEYNSLACTVEIVDDVFSAIDHIHEHGSAHTDCVVAEDQEVA
Sbjct: 605 GGKRASAILKIKEASSFHHEYNSLACTVEIVDDVFSAIDHIHEHGSAHTDCVVAEDQEVA 664

Query: 661 EIFLNQVDSAAVIHNASTRFCDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGS 720
           EIFLNQVDSAAVIHNASTRFCDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGS
Sbjct: 665 EIFLNQVDSAAVIHNASTRFCDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGS 718

Query: 721 GQVVNGDKGVAYTHKDLGVAM 742
           GQVVNGDKGVAYTHKDLGVAM
Sbjct: 725 GQVVNGDKGVAYTHKDLGVAM 718

BLAST of MS019763 vs. ExPASy TrEMBL
Match: A0A6J1CXK2 (Delta-1-pyrroline-5-carboxylate synthase OS=Momordica charantia OX=3673 GN=LOC111015124 PE=3 SV=1)

HSP 1 Score: 1343.9 bits (3477), Expect = 0.0e+00
Identity = 705/739 (95.40%), Postives = 710/739 (96.08%), Query Frame = 0

Query: 3   PTRVFVKNVKRLIVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGL 62
           P+RVF +N +RL+VKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGL
Sbjct: 3   PSRVFTRNARRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGL 62

Query: 63  GRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTD 122
           GRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTD
Sbjct: 63  GRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTD 122

Query: 123 SVFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLAL 182
           SVFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLAL
Sbjct: 123 SVFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLAL 182

Query: 183 ELKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHLGEITFGDKSRLGRGGMTAKVNA 242
           ELKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHLGEITFGDKSRLGRGGMTAKVNA
Sbjct: 183 ELKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHLGEITFGDKSRLGRGGMTAKVNA 242

Query: 243 AVYAASAGIPVVITRYMIHLVTHFEVFDTSIVTPSLIPNLVSGFATDNIIKVLQGEMKGT 302
           AVYAASAGIPVVIT                           SGFATDNIIKVLQGEMKGT
Sbjct: 243 AVYAASAGIPVVIT---------------------------SGFATDNIIKVLQGEMKGT 302

Query: 303 LFHKDAHLWTVVKEISARDMAVSARESSRRLQALKSEDRRKILLDVADALEDNEDMIIAE 362
           LFHKDAHLWTVVKEISARDMAVSARESSR+LQALKSEDRRKILLDVADALEDNEDMIIAE
Sbjct: 303 LFHKDAHLWTVVKEISARDMAVSARESSRQLQALKSEDRRKILLDVADALEDNEDMIIAE 362

Query: 363 NLADIKAAEEVGYEKSLISRLALKPGRIGTLASSIRKLADMEEPIGRILKRKELADDLIL 422
           NLADIKAAEEVGYEKSLISRLALKPGRIGTLASSIRKLADMEEPIGRILKRKELADDLIL
Sbjct: 363 NLADIKAAEEVGYEKSLISRLALKPGRIGTLASSIRKLADMEEPIGRILKRKELADDLIL 422

Query: 423 ENTSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPD 482
           ENTSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPD
Sbjct: 423 ENTSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPD 482

Query: 483 TVGEKLIGLITSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVY 542
           TVGEKLIGLITSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVY
Sbjct: 483 TVGEKLIGLITSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVY 542

Query: 543 VDKSADIEMAKRIVSDAKADYPAACNAMETLLVHKDLASNGMLIELVTELRKEGVTLYGG 602
           VDKSADIEMAKRIVSDAKADYPAACNAMETLLVHKDLASNGMLIELVTELRKEGVTLYGG
Sbjct: 543 VDKSADIEMAKRIVSDAKADYPAACNAMETLLVHKDLASNGMLIELVTELRKEGVTLYGG 602

Query: 603 KRASAILKIKEASSFHHEYNSLACTVEIVDDVFSAIDHIHEHGSAHTDCVVAEDQEVAEI 662
           KRASAILKIKEASSFHHEYNSLACTVEIVDDVFSAIDHIHEHGSAHTDCVVAEDQEVAEI
Sbjct: 603 KRASAILKIKEASSFHHEYNSLACTVEIVDDVFSAIDHIHEHGSAHTDCVVAEDQEVAEI 662

Query: 663 FLNQVDSAAVIHNASTRFCDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGSGQ 722
           FLNQVDSAAVIHNASTRFCDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGSGQ
Sbjct: 663 FLNQVDSAAVIHNASTRFCDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGSGQ 714

Query: 723 VVNGDKGVAYTHKDLGVAM 742
           VVNGDKGVAYTHKDLGVAM
Sbjct: 723 VVNGDKGVAYTHKDLGVAM 714

BLAST of MS019763 vs. ExPASy TrEMBL
Match: A0A6J1F9I8 (Delta-1-pyrroline-5-carboxylate synthase OS=Cucurbita moschata OX=3662 GN=LOC111442049 PE=3 SV=1)

HSP 1 Score: 1254.2 bits (3244), Expect = 0.0e+00
Identity = 647/735 (88.03%), Postives = 685/735 (93.20%), Query Frame = 0

Query: 3   PTRVFVKNVKRLIVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGL 62
           P+RVF KNV+RL++KVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGL
Sbjct: 3   PSRVFTKNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGL 62

Query: 63  GRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTD 122
           GRQRLRYR+LANSSFADLQNPQG+FDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTD
Sbjct: 63  GRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTD 122

Query: 123 SVFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLAL 182
           SVFCD+GFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLAL
Sbjct: 123 SVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLAL 182

Query: 183 ELKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHLGEITFGDKSRLGRGGMTAKVNA 242
           ELKADLLVLLSDVEGLYSGPP DP SKLI+TF+KEKHLGEITFGDKSR+GRGGMTAKVNA
Sbjct: 183 ELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKVNA 242

Query: 243 AVYAASAGIPVVITRYMIHLVTHFEVFDTSIVTPSLIPNLVSGFATDNIIKVLQGEMKGT 302
           AV A+ AGIPVVIT                           SGFATDNIIKVLQGEMKGT
Sbjct: 243 AVCASCAGIPVVIT---------------------------SGFATDNIIKVLQGEMKGT 302

Query: 303 LFHKDAHLWTVVKEISARDMAVSARESSRRLQALKSEDRRKILLDVADALEDNEDMIIAE 362
           LFHKDAHLWT+VKE+SAR+MAV ARESSRRLQALKSEDRRKILLDVA+AL+DNEDMI+AE
Sbjct: 303 LFHKDAHLWTLVKEVSAREMAVEARESSRRLQALKSEDRRKILLDVANALQDNEDMIMAE 362

Query: 363 NLADIKAAEEVGYEKSLISRLALKPGRIGTLASSIRKLADMEEPIGRILKRKELADDLIL 422
           N ADIKAAEE GYEKSLISRLALKPGRI  LA+SIRK+ADMEEPIGRILKRKE+A DLIL
Sbjct: 363 NNADIKAAEEAGYEKSLISRLALKPGRIELLANSIRKIADMEEPIGRILKRKEIASDLIL 422

Query: 423 ENTSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPD 482
           ENT+CSLGVLL+VFESRPDALVQIAALAIRSGNGLLLKGGKEAR+SNAALHKVITSAIPD
Sbjct: 423 ENTTCSLGVLLVVFESRPDALVQIAALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPD 482

Query: 483 TVGEKLIGLITSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVY 542
           TVG KLIGL+TSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVY
Sbjct: 483 TVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVY 542

Query: 543 VDKSADIEMAKRIVSDAKADYPAACNAMETLLVHKDLASNGMLIELVTELRKEGVTLYGG 602
           VDKSADIEMAKRIVSD+K DYPAACNAMETLLVHKDLASNG L+ELV ELRKEGVTLYGG
Sbjct: 543 VDKSADIEMAKRIVSDSKVDYPAACNAMETLLVHKDLASNGGLMELVAELRKEGVTLYGG 602

Query: 603 KRASAILKIKEASSFHHEYNSLACTVEIVDDVFSAIDHIHEHGSAHTDCVVAEDQEVAEI 662
           +RAS +L+I EASSFHHEYNSL CTVEIVDDVF+AIDHIH++GS+HTDC++ +DQEVAEI
Sbjct: 603 ERASQLLRISEASSFHHEYNSLTCTVEIVDDVFAAIDHIHQYGSSHTDCIITKDQEVAEI 662

Query: 663 FLNQVDSAAVIHNASTRFCDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGSGQ 722
           FLNQVDSAAV HNASTRFCDGARFGLGAEVG+ST RIHARGPVGVEGLLTTRWILRG+GQ
Sbjct: 663 FLNQVDSAAVFHNASTRFCDGARFGLGAEVGVSTSRIHARGPVGVEGLLTTRWILRGNGQ 710

Query: 723 VVNGDKGVAYTHKDL 738
           VVNGDKGV Y HKDL
Sbjct: 723 VVNGDKGVTYIHKDL 710

BLAST of MS019763 vs. ExPASy TrEMBL
Match: A0A6J1J3D4 (Delta-1-pyrroline-5-carboxylate synthase OS=Cucurbita maxima OX=3661 GN=LOC111481470 PE=3 SV=1)

HSP 1 Score: 1252.3 bits (3239), Expect = 0.0e+00
Identity = 644/737 (87.38%), Postives = 685/737 (92.94%), Query Frame = 0

Query: 1   VHPTRVFVKNVKRLIVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           + PTR F++N+KRL+VKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV
Sbjct: 66  IDPTRTFIRNIKRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 125

Query: 61  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYR+LANSSFADLQNPQG+FDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV
Sbjct: 126 GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 185

Query: 121 TDSVFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TDSVFCD+GFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL
Sbjct: 186 TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 245

Query: 181 ALELKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHLGEITFGDKSRLGRGGMTAKV 240
           ALELKADLLVLLSDVEGLYSGPP DP SKLI+TF+KEKHLGEITFGDKSR+GRGGMTAKV
Sbjct: 246 ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 305

Query: 241 NAAVYAASAGIPVVITRYMIHLVTHFEVFDTSIVTPSLIPNLVSGFATDNIIKVLQGEMK 300
           NAAV A+ AGIPVVIT                           SGFA DNIIKVLQGEMK
Sbjct: 306 NAAVCASCAGIPVVIT---------------------------SGFAADNIIKVLQGEMK 365

Query: 301 GTLFHKDAHLWTVVKEISARDMAVSARESSRRLQALKSEDRRKILLDVADALEDNEDMII 360
           GTLFHKDAHLWT+VKE+SAR+MAV ARESSRRLQALKSEDRRKILLDVA+AL+DNE+MII
Sbjct: 366 GTLFHKDAHLWTLVKEVSAREMAVEARESSRRLQALKSEDRRKILLDVANALQDNENMII 425

Query: 361 AENLADIKAAEEVGYEKSLISRLALKPGRIGTLASSIRKLADMEEPIGRILKRKELADDL 420
           AEN ADIKAAEE GYEKSLISRLALKPGRI  LA+SIRK+ADMEEPIGRILKR+E+A DL
Sbjct: 426 AENCADIKAAEEAGYEKSLISRLALKPGRIELLANSIRKIADMEEPIGRILKRREIASDL 485

Query: 421 ILENTSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAI 480
           ILENT+CSLGVLL+VFESRPDALVQIAALAIRSGNGLLLKGGKEAR+SNAALHKVITSAI
Sbjct: 486 ILENTTCSLGVLLVVFESRPDALVQIAALAIRSGNGLLLKGGKEARQSNAALHKVITSAI 545

Query: 481 PDTVGEKLIGLITSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICH 540
           PDTVG KLIGL+TSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICH
Sbjct: 546 PDTVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICH 605

Query: 541 VYVDKSADIEMAKRIVSDAKADYPAACNAMETLLVHKDLASNGMLIELVTELRKEGVTLY 600
           VYVDKSADIEMAKRIVSD+K DYPAACNAMETLLVHKDLASNG L+ELV ELRK+GVTLY
Sbjct: 606 VYVDKSADIEMAKRIVSDSKVDYPAACNAMETLLVHKDLASNGGLMELVAELRKDGVTLY 665

Query: 601 GGKRASAILKIKEASSFHHEYNSLACTVEIVDDVFSAIDHIHEHGSAHTDCVVAEDQEVA 660
           GG+RAS +L+I EASSFHHEYNS+ CTVEIVDDVF+AIDHIH++GS+HTDC++ +DQEVA
Sbjct: 666 GGQRASQLLRISEASSFHHEYNSVTCTVEIVDDVFAAIDHIHKYGSSHTDCIITKDQEVA 725

Query: 661 EIFLNQVDSAAVIHNASTRFCDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGS 720
           EIFLNQVDSAAV HNASTRFCDGARFGLGAEVG+ST RIHARGPVGVEGLLTTRWILRGS
Sbjct: 726 EIFLNQVDSAAVFHNASTRFCDGARFGLGAEVGVSTSRIHARGPVGVEGLLTTRWILRGS 775

Query: 721 GQVVNGDKGVAYTHKDL 738
           GQVVNGDKGV Y HKDL
Sbjct: 786 GQVVNGDKGVTYIHKDL 775

BLAST of MS019763 vs. ExPASy TrEMBL
Match: A0A6J1F4C7 (Delta-1-pyrroline-5-carboxylate synthase OS=Cucurbita moschata OX=3662 GN=LOC111442049 PE=3 SV=1)

HSP 1 Score: 1251.5 bits (3237), Expect = 0.0e+00
Identity = 645/734 (87.87%), Postives = 684/734 (93.19%), Query Frame = 0

Query: 4   TRVFVKNVKRLIVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGLG 63
           +R F++N+KRL+VKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGLG
Sbjct: 7   SRTFIRNIKRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGLG 66

Query: 64  RQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTDS 123
           RQRLRYR+LANSSFADLQNPQG+FDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTDS
Sbjct: 67  RQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTDS 126

Query: 124 VFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLALE 183
           VFCD+GFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLALE
Sbjct: 127 VFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLALE 186

Query: 184 LKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHLGEITFGDKSRLGRGGMTAKVNAA 243
           LKADLLVLLSDVEGLYSGPP DP SKLI+TF+KEKHLGEITFGDKSR+GRGGMTAKVNAA
Sbjct: 187 LKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKVNAA 246

Query: 244 VYAASAGIPVVITRYMIHLVTHFEVFDTSIVTPSLIPNLVSGFATDNIIKVLQGEMKGTL 303
           V A+ AGIPVVIT                           SGFATDNIIKVLQGEMKGTL
Sbjct: 247 VCASCAGIPVVIT---------------------------SGFATDNIIKVLQGEMKGTL 306

Query: 304 FHKDAHLWTVVKEISARDMAVSARESSRRLQALKSEDRRKILLDVADALEDNEDMIIAEN 363
           FHKDAHLWT+VKE+SAR+MAV ARESSRRLQALKSEDRRKILLDVA+AL+DNEDMI+AEN
Sbjct: 307 FHKDAHLWTLVKEVSAREMAVEARESSRRLQALKSEDRRKILLDVANALQDNEDMIMAEN 366

Query: 364 LADIKAAEEVGYEKSLISRLALKPGRIGTLASSIRKLADMEEPIGRILKRKELADDLILE 423
            ADIKAAEE GYEKSLISRLALKPGRI  LA+SIRK+ADMEEPIGRILKRKE+A DLILE
Sbjct: 367 NADIKAAEEAGYEKSLISRLALKPGRIELLANSIRKIADMEEPIGRILKRKEIASDLILE 426

Query: 424 NTSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDT 483
           NT+CSLGVLL+VFESRPDALVQIAALAIRSGNGLLLKGGKEAR+SNAALHKVITSAIPDT
Sbjct: 427 NTTCSLGVLLVVFESRPDALVQIAALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDT 486

Query: 484 VGEKLIGLITSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYV 543
           VG KLIGL+TSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYV
Sbjct: 487 VGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYV 546

Query: 544 DKSADIEMAKRIVSDAKADYPAACNAMETLLVHKDLASNGMLIELVTELRKEGVTLYGGK 603
           DKSADIEMAKRIVSD+K DYPAACNAMETLLVHKDLASNG L+ELV ELRKEGVTLYGG+
Sbjct: 547 DKSADIEMAKRIVSDSKVDYPAACNAMETLLVHKDLASNGGLMELVAELRKEGVTLYGGE 606

Query: 604 RASAILKIKEASSFHHEYNSLACTVEIVDDVFSAIDHIHEHGSAHTDCVVAEDQEVAEIF 663
           RAS +L+I EASSFHHEYNSL CTVEIVDDVF+AIDHIH++GS+HTDC++ +DQEVAEIF
Sbjct: 607 RASQLLRISEASSFHHEYNSLTCTVEIVDDVFAAIDHIHQYGSSHTDCIITKDQEVAEIF 666

Query: 664 LNQVDSAAVIHNASTRFCDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGSGQV 723
           LNQVDSAAV HNASTRFCDGARFGLGAEVG+ST RIHARGPVGVEGLLTTRWILRG+GQV
Sbjct: 667 LNQVDSAAVFHNASTRFCDGARFGLGAEVGVSTSRIHARGPVGVEGLLTTRWILRGNGQV 713

Query: 724 VNGDKGVAYTHKDL 738
           VNGDKGV Y HKDL
Sbjct: 727 VNGDKGVTYIHKDL 713

BLAST of MS019763 vs. TAIR 10
Match: AT2G39800.1 (delta1-pyrroline-5-carboxylate synthase 1 )

HSP 1 Score: 1053.9 bits (2724), Expect = 6.0e-308
Identity = 541/736 (73.51%), Postives = 617/736 (83.83%), Query Frame = 0

Query: 4   TRVFVKNVKRLIVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGLG 63
           +R F ++VKR++VKVGTAVVT   GRLALGRLGALCEQL ELNS G+EVILV+SGAVGLG
Sbjct: 7   SRAFARDVKRIVVKVGTAVVTGKGGRLALGRLGALCEQLAELNSDGFEVILVSSGAVGLG 66

Query: 64  RQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTDS 123
           RQRLRYR+L NSSFADLQ PQ + DGKACA VGQSSLMA Y+TMF QLDVT++QLLV DS
Sbjct: 67  RQRLRYRQLVNSSFADLQKPQTELDGKACAGVGQSSLMAYYETMFDQLDVTAAQLLVNDS 126

Query: 124 VFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLALE 183
            F D  FR+QL+ETV S+LDLRVIPIFNENDA+STR+ PY+DSSGIFWDNDSLA LLALE
Sbjct: 127 SFRDKDFRKQLNETVKSMLDLRVIPIFNENDAISTRRAPYQDSSGIFWDNDSLAALLALE 186

Query: 184 LKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHLGEITFGDKSRLGRGGMTAKVNAA 243
           LKADLL+LLSDVEGLY+GPP DPNSKLI TF+KEKH  EITFGDKSRLGRGGMTAKV AA
Sbjct: 187 LKADLLILLSDVEGLYTGPPSDPNSKLIHTFVKEKHQDEITFGDKSRLGRGGMTAKVKAA 246

Query: 244 VYAASAGIPVVITRYMIHLVTHFEVFDTSIVTPSLIPNLVSGFATDNIIKVLQGEMKGTL 303
           V AA AGIPV+IT                           SG++ +NI KVL+G   GTL
Sbjct: 247 VNAAYAGIPVIIT---------------------------SGYSAENIDKVLRGLRVGTL 306

Query: 304 FHKDAHLWTVVKEISARDMAVSARESSRRLQALKSEDRRKILLDVADALEDNEDMIIAEN 363
           FH+DA LW  + + +ARDMAV+ARESSR+LQAL SEDR+KILLD+ADALE N   I AEN
Sbjct: 307 FHQDARLWAPITDSNARDMAVAARESSRKLQALSSEDRKKILLDIADALEANVTTIKAEN 366

Query: 364 LADIKAAEEVGYEKSLISRLALKPGRIGTLASSIRKLADMEEPIGRILKRKELADDLILE 423
             D+ +A+E G E+S+++RL + PG+I +LA+S+RKLADME+PIGR+LK+ E+AD L+LE
Sbjct: 367 ELDVASAQEAGLEESMVARLVMTPGKISSLAASVRKLADMEDPIGRVLKKTEVADGLVLE 426

Query: 424 NTSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDT 483
            TS  LGVLLIVFESRPDALVQIA+LAIRSGNGLLLKGGKEARRSNA LHKVIT AIP+T
Sbjct: 427 KTSSPLGVLLIVFESRPDALVQIASLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPET 486

Query: 484 VGEKLIGLITSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYV 543
           VG KLIGL+TSREEIPDLLKLDDVIDLVIPRGSNKLV+QIK +TKIPVLGH+DGICHVYV
Sbjct: 487 VGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVTQIKNTTKIPVLGHADGICHVYV 546

Query: 544 DKSADIEMAKRIVSDAKADYPAACNAMETLLVHKDLASNGMLIELVTELRKEGVTLYGGK 603
           DK+ D +MAKRIVSDAK DYPAACNAMETLLVHKDL  N +L EL+  L+  GVTLYGG 
Sbjct: 547 DKACDTDMAKRIVSDAKLDYPAACNAMETLLVHKDLEQNAVLNELIFALQSNGVTLYGGP 606

Query: 604 RASAILKIKEASSFHHEYNSLACTVEIVDDVFSAIDHIHEHGSAHTDCVVAEDQEVAEIF 663
           RAS IL I EA SF+HEY + ACTVE+V+DV+ AIDHIH HGSAHTDC+V ED EVAE+F
Sbjct: 607 RASKILNIPEARSFNHEYCAKACTVEVVEDVYGAIDHIHRHGSAHTDCIVTEDHEVAELF 666

Query: 664 LNQVDSAAVIHNASTRFCDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGSGQV 723
           L QVDSAAV HNASTRF DG RFGLGAEVG+STGRIHARGPVGVEGLLTTRWI+RG GQV
Sbjct: 667 LRQVDSAAVFHNASTRFSDGFRFGLGAEVGVSTGRIHARGPVGVEGLLTTRWIMRGKGQV 715

Query: 724 VNGDKGVAYTHKDLGV 740
           V+GD G+ YTH+D+ +
Sbjct: 727 VDGDNGIVYTHQDIPI 715

BLAST of MS019763 vs. TAIR 10
Match: AT2G39800.4 (delta1-pyrroline-5-carboxylate synthase 1 )

HSP 1 Score: 1053.9 bits (2724), Expect = 6.0e-308
Identity = 541/736 (73.51%), Postives = 617/736 (83.83%), Query Frame = 0

Query: 4   TRVFVKNVKRLIVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGLG 63
           +R F ++VKR++VKVGTAVVT   GRLALGRLGALCEQL ELNS G+EVILV+SGAVGLG
Sbjct: 7   SRAFARDVKRIVVKVGTAVVTGKGGRLALGRLGALCEQLAELNSDGFEVILVSSGAVGLG 66

Query: 64  RQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTDS 123
           RQRLRYR+L NSSFADLQ PQ + DGKACA VGQSSLMA Y+TMF QLDVT++QLLV DS
Sbjct: 67  RQRLRYRQLVNSSFADLQKPQTELDGKACAGVGQSSLMAYYETMFDQLDVTAAQLLVNDS 126

Query: 124 VFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLALE 183
            F D  FR+QL+ETV S+LDLRVIPIFNENDA+STR+ PY+DSSGIFWDNDSLA LLALE
Sbjct: 127 SFRDKDFRKQLNETVKSMLDLRVIPIFNENDAISTRRAPYQDSSGIFWDNDSLAALLALE 186

Query: 184 LKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHLGEITFGDKSRLGRGGMTAKVNAA 243
           LKADLL+LLSDVEGLY+GPP DPNSKLI TF+KEKH  EITFGDKSRLGRGGMTAKV AA
Sbjct: 187 LKADLLILLSDVEGLYTGPPSDPNSKLIHTFVKEKHQDEITFGDKSRLGRGGMTAKVKAA 246

Query: 244 VYAASAGIPVVITRYMIHLVTHFEVFDTSIVTPSLIPNLVSGFATDNIIKVLQGEMKGTL 303
           V AA AGIPV+IT                           SG++ +NI KVL+G   GTL
Sbjct: 247 VNAAYAGIPVIIT---------------------------SGYSAENIDKVLRGLRVGTL 306

Query: 304 FHKDAHLWTVVKEISARDMAVSARESSRRLQALKSEDRRKILLDVADALEDNEDMIIAEN 363
           FH+DA LW  + + +ARDMAV+ARESSR+LQAL SEDR+KILLD+ADALE N   I AEN
Sbjct: 307 FHQDARLWAPITDSNARDMAVAARESSRKLQALSSEDRKKILLDIADALEANVTTIKAEN 366

Query: 364 LADIKAAEEVGYEKSLISRLALKPGRIGTLASSIRKLADMEEPIGRILKRKELADDLILE 423
             D+ +A+E G E+S+++RL + PG+I +LA+S+RKLADME+PIGR+LK+ E+AD L+LE
Sbjct: 367 ELDVASAQEAGLEESMVARLVMTPGKISSLAASVRKLADMEDPIGRVLKKTEVADGLVLE 426

Query: 424 NTSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDT 483
            TS  LGVLLIVFESRPDALVQIA+LAIRSGNGLLLKGGKEARRSNA LHKVIT AIP+T
Sbjct: 427 KTSSPLGVLLIVFESRPDALVQIASLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPET 486

Query: 484 VGEKLIGLITSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYV 543
           VG KLIGL+TSREEIPDLLKLDDVIDLVIPRGSNKLV+QIK +TKIPVLGH+DGICHVYV
Sbjct: 487 VGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVTQIKNTTKIPVLGHADGICHVYV 546

Query: 544 DKSADIEMAKRIVSDAKADYPAACNAMETLLVHKDLASNGMLIELVTELRKEGVTLYGGK 603
           DK+ D +MAKRIVSDAK DYPAACNAMETLLVHKDL  N +L EL+  L+  GVTLYGG 
Sbjct: 547 DKACDTDMAKRIVSDAKLDYPAACNAMETLLVHKDLEQNAVLNELIFALQSNGVTLYGGP 606

Query: 604 RASAILKIKEASSFHHEYNSLACTVEIVDDVFSAIDHIHEHGSAHTDCVVAEDQEVAEIF 663
           RAS IL I EA SF+HEY + ACTVE+V+DV+ AIDHIH HGSAHTDC+V ED EVAE+F
Sbjct: 607 RASKILNIPEARSFNHEYCAKACTVEVVEDVYGAIDHIHRHGSAHTDCIVTEDHEVAELF 666

Query: 664 LNQVDSAAVIHNASTRFCDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGSGQV 723
           L QVDSAAV HNASTRF DG RFGLGAEVG+STGRIHARGPVGVEGLLTTRWI+RG GQV
Sbjct: 667 LRQVDSAAVFHNASTRFSDGFRFGLGAEVGVSTGRIHARGPVGVEGLLTTRWIMRGKGQV 715

Query: 724 VNGDKGVAYTHKDLGV 740
           V+GD G+ YTH+D+ +
Sbjct: 727 VDGDNGIVYTHQDIPI 715

BLAST of MS019763 vs. TAIR 10
Match: AT3G55610.1 (delta 1-pyrroline-5-carboxylate synthase 2 )

HSP 1 Score: 1050.8 bits (2716), Expect = 5.1e-307
Identity = 541/736 (73.51%), Postives = 618/736 (83.97%), Query Frame = 0

Query: 4   TRVFVKNVKRLIVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGLG 63
           +R F K+VKR++VKVGTAVVT   GRLALGRLGA+CEQL ELNS G+EVILV+SGAVGLG
Sbjct: 7   SRAFAKDVKRIVVKVGTAVVTGKGGRLALGRLGAICEQLAELNSDGFEVILVSSGAVGLG 66

Query: 64  RQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTDS 123
           RQRLRYR+L NSSFADLQ PQ + DGKACA VGQSSLMA Y+TMF QLDVT +Q+LVTDS
Sbjct: 67  RQRLRYRQLVNSSFADLQKPQMELDGKACAGVGQSSLMAYYETMFDQLDVTVAQMLVTDS 126

Query: 124 VFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLALE 183
            F D  FR+QLSETV ++L +RVIP+FNENDA+STR+ PY+DS+GIFWDNDSLA LL+LE
Sbjct: 127 SFRDKDFRKQLSETVKAMLRMRVIPVFNENDAISTRRAPYKDSTGIFWDNDSLAALLSLE 186

Query: 184 LKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHLGEITFGDKSRLGRGGMTAKVNAA 243
           LKADLL+LLSDVEGLY+GPP D  SKLI TFIKEKH  EITFG+KS+LGRGGMTAKV AA
Sbjct: 187 LKADLLILLSDVEGLYTGPPSDSTSKLIHTFIKEKHQDEITFGEKSKLGRGGMTAKVKAA 246

Query: 244 VYAASAGIPVVITRYMIHLVTHFEVFDTSIVTPSLIPNLVSGFATDNIIKVLQGEMKGTL 303
           V AA  G+PV+IT                           SG+A +NI KVL+G   GTL
Sbjct: 247 VNAAYGGVPVIIT---------------------------SGYAAENISKVLRGLRVGTL 306

Query: 304 FHKDAHLWTVVKEISARDMAVSARESSRRLQALKSEDRRKILLDVADALEDNEDMIIAEN 363
           FH+DAHLW  V + ++RDMAV+ARESSR+LQAL SEDR++IL D+A+ALE NE  I AEN
Sbjct: 307 FHQDAHLWAPVVDTTSRDMAVAARESSRKLQALSSEDRKQILHDIANALEVNEKTIKAEN 366

Query: 364 LADIKAAEEVGYEKSLISRLALKPGRIGTLASSIRKLADMEEPIGRILKRKELADDLILE 423
             D+ AA+E GYE+SL++RL +KPG+I +LA+S+R+LA+ME+PIGR+LK+ ++ADDLILE
Sbjct: 367 DLDVAAAQEAGYEESLVARLVMKPGKISSLAASVRQLAEMEDPIGRVLKKTQVADDLILE 426

Query: 424 NTSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDT 483
            TS  +GVLLIVFESRPDALVQIA+LAIRSGNGLLLKGGKEARRSNA LHKVIT AIP+T
Sbjct: 427 KTSSPIGVLLIVFESRPDALVQIASLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPET 486

Query: 484 VGEKLIGLITSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYV 543
           VG KLIGL+TSREEIPDLLKLDDVIDLVIPRGSNKLVSQIK STKIPVLGH+DGICHVYV
Sbjct: 487 VGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKNSTKIPVLGHADGICHVYV 546

Query: 544 DKSADIEMAKRIVSDAKADYPAACNAMETLLVHKDLASNGMLIELVTELRKEGVTLYGGK 603
           DKS  ++MAKRIVSDAK DYPAACNAMETLLVHKDL  NG L +L+  L+ +GVTLYGG 
Sbjct: 547 DKSGKLDMAKRIVSDAKLDYPAACNAMETLLVHKDLEQNGFLDDLIYVLQTKGVTLYGGP 606

Query: 604 RASAILKIKEASSFHHEYNSLACTVEIVDDVFSAIDHIHEHGSAHTDCVVAEDQEVAEIF 663
           RASA L I E  SFHHEY+S ACTVEIV+DV+ AIDHIH+HGSAHTDC+V ED EVAEIF
Sbjct: 607 RASAKLNIPETKSFHHEYSSKACTVEIVEDVYGAIDHIHQHGSAHTDCIVTEDSEVAEIF 666

Query: 664 LNQVDSAAVIHNASTRFCDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGSGQV 723
           L QVDSAAV HNASTRF DG RFGLGAEVGIST RIHARGPVGVEGLLTTRWI+RG GQV
Sbjct: 667 LRQVDSAAVFHNASTRFSDGFRFGLGAEVGISTSRIHARGPVGVEGLLTTRWIMRGKGQV 715

Query: 724 VNGDKGVAYTHKDLGV 740
           V+GD G+ YTHKDL V
Sbjct: 727 VDGDNGIVYTHKDLPV 715

BLAST of MS019763 vs. TAIR 10
Match: AT2G39800.3 (delta1-pyrroline-5-carboxylate synthase 1 )

HSP 1 Score: 1042.7 bits (2695), Expect = 1.4e-304
Identity = 539/736 (73.23%), Postives = 614/736 (83.42%), Query Frame = 0

Query: 4   TRVFVKNVKRLIVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGLG 63
           +R F ++VKR++VKVGTAVVT   GRLALGRLGALCEQL ELNS G+EVILV+SGAVGLG
Sbjct: 7   SRAFARDVKRIVVKVGTAVVTGKGGRLALGRLGALCEQLAELNSDGFEVILVSSGAVGLG 66

Query: 64  RQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTDS 123
           RQRLRYR+L NSSFADLQ PQ + DGKACA VGQSSLMA Y+TMF QLDVT++QLLV DS
Sbjct: 67  RQRLRYRQLVNSSFADLQKPQTELDGKACAGVGQSSLMAYYETMFDQLDVTAAQLLVNDS 126

Query: 124 VFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLALE 183
            F D  FR+QL+ETV S+LDLRVIPIFNENDA+STR+ PY+DSSGIFWDNDSLA LLALE
Sbjct: 127 SFRDKDFRKQLNETVKSMLDLRVIPIFNENDAISTRRAPYQDSSGIFWDNDSLAALLALE 186

Query: 184 LKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHLGEITFGDKSRLGRGGMTAKVNAA 243
           LKADLL+LLSDVEGLY+GPP DPNSKLI TF+KEKH  EITFGDKSRLGRGGMTAKV AA
Sbjct: 187 LKADLLILLSDVEGLYTGPPSDPNSKLIHTFVKEKHQDEITFGDKSRLGRGGMTAKVKAA 246

Query: 244 VYAASAGIPVVITRYMIHLVTHFEVFDTSIVTPSLIPNLVSGFATDNIIKVLQGEMKGTL 303
           V AA AGIPV+IT                           SG++ +NI KVL+G   GTL
Sbjct: 247 VNAAYAGIPVIIT---------------------------SGYSAENIDKVLRGLRVGTL 306

Query: 304 FHKDAHLWTVVKEISARDMAVSARESSRRLQALKSEDRRKILLDVADALEDNEDMIIAEN 363
           FH+DA LW  + + +ARDMAV+ARESSR+LQAL SEDR+KILLD+ADALE N   I AEN
Sbjct: 307 FHQDARLWAPITDSNARDMAVAARESSRKLQALSSEDRKKILLDIADALEANVTTIKAEN 366

Query: 364 LADIKAAEEVGYEKSLISRLALKPGRIGTLASSIRKLADMEEPIGRILKRKELADDLILE 423
             D+ +A+E G E+S+++RL + PG+I +LA+S+RKLADME+PIGR+LK+ E+AD L+LE
Sbjct: 367 ELDVASAQEAGLEESMVARLVMTPGKISSLAASVRKLADMEDPIGRVLKKTEVADGLVLE 426

Query: 424 NTSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDT 483
            TS  LGVLLIVFESRPDALVQIA+LAIRSGNGLLLKGGKEARRSNA LHKVIT AIP+T
Sbjct: 427 KTSSPLGVLLIVFESRPDALVQIASLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPET 486

Query: 484 VGEKLIGLITSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYV 543
           VG KLIGL+TSREEIPDLLKLDDVIDLVIPRGSNKLV+QIK +TKIPVL   DGICHVYV
Sbjct: 487 VGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVTQIKNTTKIPVL---DGICHVYV 546

Query: 544 DKSADIEMAKRIVSDAKADYPAACNAMETLLVHKDLASNGMLIELVTELRKEGVTLYGGK 603
           DK+ D +MAKRIVSDAK DYPAACNAMETLLVHKDL  N +L EL+  L+  GVTLYGG 
Sbjct: 547 DKACDTDMAKRIVSDAKLDYPAACNAMETLLVHKDLEQNAVLNELIFALQSNGVTLYGGP 606

Query: 604 RASAILKIKEASSFHHEYNSLACTVEIVDDVFSAIDHIHEHGSAHTDCVVAEDQEVAEIF 663
           RAS IL I EA SF+HEY + ACTVE+V+DV+ AIDHIH HGSAHTDC+V ED EVAE+F
Sbjct: 607 RASKILNIPEARSFNHEYCAKACTVEVVEDVYGAIDHIHRHGSAHTDCIVTEDHEVAELF 666

Query: 664 LNQVDSAAVIHNASTRFCDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGSGQV 723
           L QVDSAAV HNASTRF DG RFGLGAEVG+STGRIHARGPVGVEGLLTTRWI+RG GQV
Sbjct: 667 LRQVDSAAVFHNASTRFSDGFRFGLGAEVGVSTGRIHARGPVGVEGLLTTRWIMRGKGQV 712

Query: 724 VNGDKGVAYTHKDLGV 740
           V+GD G+ YTH+D+ +
Sbjct: 727 VDGDNGIVYTHQDIPI 712

BLAST of MS019763 vs. TAIR 10
Match: AT2G39800.2 (delta1-pyrroline-5-carboxylate synthase 1 )

HSP 1 Score: 909.4 bits (2349), Expect = 1.8e-264
Identity = 465/639 (72.77%), Postives = 532/639 (83.26%), Query Frame = 0

Query: 101 MALYDTMFSQLDVTSSQLLVTDSVFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRK 160
           MA Y+TMF QLDVT++QLLV DS F D  FR+QL+ETV S+LDLRVIPIFNENDA+STR+
Sbjct: 1   MAYYETMFDQLDVTAAQLLVNDSSFRDKDFRKQLNETVKSMLDLRVIPIFNENDAISTRR 60

Query: 161 TPYEDSSGIFWDNDSLAGLLALELKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHL 220
            PY+DSSGIFWDNDSLA LLALELKADLL+LLSDVEGLY+GPP DPNSKLI TF+KEKH 
Sbjct: 61  APYQDSSGIFWDNDSLAALLALELKADLLILLSDVEGLYTGPPSDPNSKLIHTFVKEKHQ 120

Query: 221 GEITFGDKSRLGRGGMTAKVNAAVYAASAGIPVVITRYMIHLVTHFEVFDTSIVTPSLIP 280
            EITFGDKSRLGRGGMTAKV AAV AA AGIPV+IT                        
Sbjct: 121 DEITFGDKSRLGRGGMTAKVKAAVNAAYAGIPVIIT------------------------ 180

Query: 281 NLVSGFATDNIIKVLQGEMKGTLFHKDAHLWTVVKEISARDMAVSARESSRRLQALKSED 340
              SG++ +NI KVL+G   GTLFH+DA LW  + + +ARDMAV+ARESSR+LQAL SED
Sbjct: 181 ---SGYSAENIDKVLRGLRVGTLFHQDARLWAPITDSNARDMAVAARESSRKLQALSSED 240

Query: 341 RRKILLDVADALEDNEDMIIAENLADIKAAEEVGYEKSLISRLALKPGRIGTLASSIRKL 400
           R+KILLD+ADALE N   I AEN  D+ +A+E G E+S+++RL + PG+I +LA+S+RKL
Sbjct: 241 RKKILLDIADALEANVTTIKAENELDVASAQEAGLEESMVARLVMTPGKISSLAASVRKL 300

Query: 401 ADMEEPIGRILKRKELADDLILENTSCSLGVLLIVFESRPDALVQIAALAIRSGNGLLLK 460
           ADME+PIGR+LK+ E+AD L+LE TS  LGVLLIVFESRPDALVQIA+LAIRSGNGLLLK
Sbjct: 301 ADMEDPIGRVLKKTEVADGLVLEKTSSPLGVLLIVFESRPDALVQIASLAIRSGNGLLLK 360

Query: 461 GGKEARRSNAALHKVITSAIPDTVGEKLIGLITSREEIPDLLKLDDVIDLVIPRGSNKLV 520
           GGKEARRSNA LHKVIT AIP+TVG KLIGL+TSREEIPDLLKLDDVIDLVIPRGSNKLV
Sbjct: 361 GGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLV 420

Query: 521 SQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDAKADYPAACNAMETLLVHKDLA 580
           +QIK +TKIPVLGH+DGICHVYVDK+ D +MAKRIVSDAK DYPAACNAMETLLVHKDL 
Sbjct: 421 TQIKNTTKIPVLGHADGICHVYVDKACDTDMAKRIVSDAKLDYPAACNAMETLLVHKDLE 480

Query: 581 SNGMLIELVTELRKEGVTLYGGKRASAILKIKEASSFHHEYNSLACTVEIVDDVFSAIDH 640
            N +L EL+  L+  GVTLYGG RAS IL I EA SF+HEY + ACTVE+V+DV+ AIDH
Sbjct: 481 QNAVLNELIFALQSNGVTLYGGPRASKILNIPEARSFNHEYCAKACTVEVVEDVYGAIDH 540

Query: 641 IHEHGSAHTDCVVAEDQEVAEIFLNQVDSAAVIHNASTRFCDGARFGLGAEVGISTGRIH 700
           IH HGSAHTDC+V ED EVAE+FL QVDSAAV HNASTRF DG RFGLGAEVG+STGRIH
Sbjct: 541 IHRHGSAHTDCIVTEDHEVAELFLRQVDSAAVFHNASTRFSDGFRFGLGAEVGVSTGRIH 600

Query: 701 ARGPVGVEGLLTTRWILRGSGQVVNGDKGVAYTHKDLGV 740
           ARGPVGVEGLLTTRWI+RG GQVV+GD G+ YTH+D+ +
Sbjct: 601 ARGPVGVEGLLTTRWIMRGKGQVVDGDNGIVYTHQDIPI 612

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022145741.10.0e+0096.22delta-1-pyrroline-5-carboxylate synthase-like isoform X1 [Momordica charantia][more]
XP_022145742.10.0e+0095.40delta-1-pyrroline-5-carboxylate synthase-like isoform X2 [Momordica charantia][more]
XP_038905634.10.0e+0089.15delta-1-pyrroline-5-carboxylate synthase-like isoform X1 [Benincasa hispida][more]
XP_038905635.10.0e+0089.39delta-1-pyrroline-5-carboxylate synthase-like isoform X2 [Benincasa hispida][more]
XP_023529105.10.0e+0088.16delta-1-pyrroline-5-carboxylate synthase-like isoform X1 [Cucurbita pepo subsp. ... [more]
Match NameE-valueIdentityDescription
O653610.0e+0075.54Delta-1-pyrroline-5-carboxylate synthase OS=Mesembryanthemum crystallinum OX=354... [more]
O040150.0e+0075.85Delta-1-pyrroline-5-carboxylate synthase OS=Actinidia deliciosa OX=3627 PE=2 SV=... [more]
Q964806.2e-31074.49Delta-1-pyrroline-5-carboxylate synthase OS=Solanum lycopersicum OX=4081 GN=PRO2... [more]
P548878.4e-30773.51Delta-1-pyrroline-5-carboxylate synthase A OS=Arabidopsis thaliana OX=3702 GN=P5... [more]
P548887.1e-30673.51Delta-1-pyrroline-5-carboxylate synthase B OS=Arabidopsis thaliana OX=3702 GN=P5... [more]
Match NameE-valueIdentityDescription
A0A6J1CX790.0e+0096.22Delta-1-pyrroline-5-carboxylate synthase OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A6J1CXK20.0e+0095.40Delta-1-pyrroline-5-carboxylate synthase OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A6J1F9I80.0e+0088.03Delta-1-pyrroline-5-carboxylate synthase OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1J3D40.0e+0087.38Delta-1-pyrroline-5-carboxylate synthase OS=Cucurbita maxima OX=3661 GN=LOC11148... [more]
A0A6J1F4C70.0e+0087.87Delta-1-pyrroline-5-carboxylate synthase OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
Match NameE-valueIdentityDescription
AT2G39800.16.0e-30873.51delta1-pyrroline-5-carboxylate synthase 1 [more]
AT2G39800.46.0e-30873.51delta1-pyrroline-5-carboxylate synthase 1 [more]
AT3G55610.15.1e-30773.51delta 1-pyrroline-5-carboxylate synthase 2 [more]
AT2G39800.31.4e-30473.23delta1-pyrroline-5-carboxylate synthase 1 [more]
AT2G39800.21.8e-26472.77delta1-pyrroline-5-carboxylate synthase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001057Glutamate/acetylglutamate kinasePRINTSPR00474GLU5KINASEcoord: 231..251
score: 56.44
coord: 133..154
score: 56.4
coord: 49..63
score: 64.94
coord: 93..121
score: 55.43
coord: 172..199
score: 64.55
IPR001048Aspartate/glutamate/uridylate kinasePFAMPF00696AA_kinasecoord: 12..256
e-value: 3.7E-35
score: 121.7
IPR005766Delta l-pyrroline-5-carboxylate synthetaseTIGRFAMTIGR01092TIGR01092coord: 283..739
e-value: 2.1E-241
score: 801.0
IPR005766Delta l-pyrroline-5-carboxylate synthetasePIRSFPIRSF036429P5C_synthetasecoord: 1..270
e-value: 1.0E-125
score: 418.2
coord: 271..741
e-value: 1.1E-228
score: 758.7
IPR015590Aldehyde dehydrogenase domainPFAMPF00171Aldedhcoord: 321..584
e-value: 7.7E-6
score: 24.7
IPR036393Acetylglutamate kinase-like superfamilyGENE3D3.40.1160.10coord: 3..311
e-value: 7.6E-70
score: 237.4
IPR036393Acetylglutamate kinase-like superfamilySUPERFAMILY53633Carbamate kinase-likecoord: 12..258
IPR016163Aldehyde dehydrogenase, C-terminalGENE3D3.40.309.10Aldehyde Dehydrogenase; Chain A, domain 2coord: 536..682
e-value: 8.9E-150
score: 500.8
IPR016162Aldehyde dehydrogenase, N-terminalGENE3D3.40.605.10Aldehyde Dehydrogenase; Chain A, domain 1coord: 327..722
e-value: 8.9E-150
score: 500.8
IPR000965GPR domainTIGRFAMTIGR00407TIGR00407coord: 326..713
e-value: 3.1E-133
score: 442.4
IPR000965GPR domainHAMAPMF_00412ProAcoord: 318..724
score: 34.089836
IPR000965GPR domainCDDcd07079ALDH_F18-19_ProA-GPRcoord: 320..720
e-value: 0.0
score: 562.825
NoneNo IPR availablePIRSRPIRSR016496-1PIRSR016496-1coord: 14..255
e-value: 5.8E-9
score: 33.0
NoneNo IPR availablePANTHERPTHR11063GLUTAMATE SEMIALDEHYDE DEHYDROGENASEcoord: 2..257
coord: 279..739
NoneNo IPR availablePANTHERPTHR11063:SF18DELTA-1-PYRROLINE-5-CARBOXYLATE SYNTHASEcoord: 2..257
coord: 279..739
IPR019797Glutamate 5-kinase, conserved sitePROSITEPS00902GLUTAMATE_5_KINASEcoord: 229..246
IPR016161Aldehyde/histidinol dehydrogenaseSUPERFAMILY53720ALDH-likecoord: 320..723

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS019763.1MS019763.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006561 proline biosynthetic process
molecular_function GO:0003824 catalytic activity
molecular_function GO:0004349 glutamate 5-kinase activity
molecular_function GO:0004350 glutamate-5-semialdehyde dehydrogenase activity
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016620 oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor