MS019737 (gene) Bitter gourd (TR) v1

BLAST of MS019737 vs. NCBI nr
Match: XP_022145578.1 (E3 ubiquitin-protein ligase PRT6 [Momordica charantia] >XP_022145579.1 E3 ubiquitin-protein ligase PRT6 [Momordica charantia] >XP_022145580.1 E3 ubiquitin-protein ligase PRT6 [Momordica charantia])

HSP 1 Score: 4013.0 bits (10406), Expect = 0.0e+00
Identity = 2029/2040 (99.46%), Postives = 2033/2040 (99.66%), Query Frame = 0

Query: 1    MDDMDIGSPSESAPLKPRDRIVRRLALLGVPEELLDQLQPGLVSFVKDNKILIPQLVSAI 60
            MDDM+IGSPSESAPLKPRDRIVRRLALLGVPEELLDQLQPGLVSFVKDNKILIPQLVSAI
Sbjct: 1    MDDMNIGSPSESAPLKPRDRIVRRLALLGVPEELLDQLQPGLVSFVKDNKILIPQLVSAI 60

Query: 61   LPTDVEVAEVIRDAKAGSKKSMAGPTMKNQFRESMVWLQWLMFEGEPTFALKNLSKMSFG 120
            LPTDVEVAEVIRDAKAGSKKS+AGPTMKNQFRESMVWLQWLMFEGEPTFALKNLSKMSFG
Sbjct: 61   LPTDVEVAEVIRDAKAGSKKSVAGPTMKNQFRESMVWLQWLMFEGEPTFALKNLSKMSFG 120

Query: 121  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVT 180
            QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVT
Sbjct: 121  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVT 180

Query: 181  AWKREGFCSKHKGAERIQPLPEEFVESVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 240
            AWKREGFCSKHKGAERIQPLPEEFVESVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV
Sbjct: 181  AWKREGFCSKHKGAERIQPLPEEFVESVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 240

Query: 241  MEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISLAGFLDILVRLERLLTDIVVKK 300
            MEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISLAGFLDILVRLERLLTDIVVKK
Sbjct: 241  MEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISLAGFLDILVRLERLLTDIVVKK 300

Query: 301  VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHALKKYPLLPTFSVQIFTVPT 360
            VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHALKKYPLLPTFSVQIFTVPT
Sbjct: 301  VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHALKKYPLLPTFSVQIFTVPT 360

Query: 361  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAV 420
            LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAV
Sbjct: 361  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAV 420

Query: 421  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHTL 480
            VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHTL
Sbjct: 421  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHTL 480

Query: 481  LVNEAFSAASSSSSSEETADAMSFHAFKQNPDDMDGIRHAKVGRLSQESAACNVFGRSST 540
            LVNEAFSAASSSSSSEETADAMSFHAFKQNPDDMD IRHAKVGRLSQESAACNVFGRSST
Sbjct: 481  LVNEAFSAASSSSSSEETADAMSFHAFKQNPDDMDSIRHAKVGRLSQESAACNVFGRSST 540

Query: 541  VTSASRVDEVCSDTISSTIMWLTYECLKVIDSWLGTENVLGGFPNMFAENASMASSCKVY 600
            VTSASRVDEVCSDTISSTIMWLTYECLKVIDSWLGTEN LGGFPNMFAENASMASSCKVY
Sbjct: 541  VTSASRVDEVCSDTISSTIMWLTYECLKVIDSWLGTENALGGFPNMFAENASMASSCKVY 600

Query: 601  SLRKPSALESRKISSKVEKGKFIFEKVAGKSKDHNRQYSSRIYSGLQMSIDNEHGVSLGE 660
            SLRKPSALESRKISSKVEKGKFIFEKVAGKSKDHNRQYSSRIYSGLQMSIDNEHGVSLGE
Sbjct: 601  SLRKPSALESRKISSKVEKGKFIFEKVAGKSKDHNRQYSSRIYSGLQMSIDNEHGVSLGE 660

Query: 661  DNQLMDVTNDTVTVEDYSMETDALHFLSFSAWPNIVYDVSSQDISIHIPLHRLLSLLLQK 720
            DNQLMDVTNDTVTVEDY+METDALHFLSFSAWPNIVYDVSSQDISIHIPLHRLLSLLLQK
Sbjct: 661  DNQLMDVTNDTVTVEDYAMETDALHFLSFSAWPNIVYDVSSQDISIHIPLHRLLSLLLQK 720

Query: 721  ALRSSFSESVVPSATGASSSNLSFEYVDFFKSVLTDYHPYGFSAFVMEHPLRIKVFCAEV 780
            ALRSSFSESVVPSATGASSSNLSFEYVDFFKSVLTDYHPYGFSAFVMEHPLRIKVFCAEV
Sbjct: 721  ALRSSFSESVVPSATGASSSNLSFEYVDFFKSVLTDYHPYGFSAFVMEHPLRIKVFCAEV 780

Query: 781  NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPELYISRILERFRLLD 840
            NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPELYISRILERFRLLD
Sbjct: 781  NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPELYISRILERFRLLD 840

Query: 841  YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQL 900
            YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQL
Sbjct: 841  YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQL 900

Query: 901  VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLRWKYWKELDLYHPRWSLRDLQIA 960
            VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLRWKYWKELDLYHPRWSLRDLQIA
Sbjct: 901  VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLRWKYWKELDLYHPRWSLRDLQIA 960

Query: 961  EERYLRSCGASALTSQLPKWTKIYPPFRGLARIATCKAVLQIIRAVLFYSVFTDKSTKSR 1020
            EERYLRSCGASALTSQLPKWTKIYPPFRGLARIATCKAVLQIIRAVLFYSVFTDKSTKSR
Sbjct: 961  EERYLRSCGASALTSQLPKWTKIYPPFRGLARIATCKAVLQIIRAVLFYSVFTDKSTKSR 1020

Query: 1021 APDSVLLTALHLLALALDICFQRKESSDQSFCSEDSIPLLLFADEEVDEGLTYGLGRQSL 1080
            APDSVLLTALHLLALALDICFQRKESSDQSFCSEDSIPLLLFADEEVDEGLTYGLGRQSL
Sbjct: 1021 APDSVLLTALHLLALALDICFQRKESSDQSFCSEDSIPLLLFADEEVDEGLTYGLGRQSL 1080

Query: 1081 LSLLILLMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDSRCMGKIRQHAPEILGYL 1140
            LSLLILLMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDSRCMGKIRQHAPEILGYL
Sbjct: 1081 LSLLILLMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDSRCMGKIRQHAPEILGYL 1140

Query: 1141 SQSLPSSDPNSLSETSDSDKLKAKARERQAAILEKMRAEQYKFLASVDSSVEDDAEFGQE 1200
            SQSLPSSDPNSLSETSDSDKLKAKARERQAAILEKMRAEQYKFLASVDSSVEDDAEFGQE
Sbjct: 1141 SQSLPSSDPNSLSETSDSDKLKAKARERQAAILEKMRAEQYKFLASVDSSVEDDAEFGQE 1200

Query: 1201 SEKPSVSDSAEQSETVCSLCHDSSSSVPISYLILLQKSKLVSLIDRGTVSWDQPYWRDEH 1260
            SEKPSVSDSAEQSETVCSLCHDSSSSVPISYLILLQKSKLVSLIDRGTVSWDQPYWRDEH
Sbjct: 1201 SEKPSVSDSAEQSETVCSLCHDSSSSVPISYLILLQKSKLVSLIDRGTVSWDQPYWRDEH 1260

Query: 1261 ASSIPRRNLDQSGLSTSSGGSGGISSPQLAELIQNAVKEYASHGLPGEVGAFLDFVKSHF 1320
            ASSIPRRNLDQS LSTS   SGGISSPQLA+LIQNAVKEYASHGLPGEVGAFLDFVKSHF
Sbjct: 1261 ASSIPRRNLDQSELSTS---SGGISSPQLAQLIQNAVKEYASHGLPGEVGAFLDFVKSHF 1320

Query: 1321 PPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHENMHSKLNEDENFSKVASSLV 1380
            PPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHENMHSKLNEDEN SKVASSLV
Sbjct: 1321 PPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHENMHSKLNEDENLSKVASSLV 1380

Query: 1381 TEDSKSVLHVKYIAALSRELAENHSTSESAHNVSMPVESLQPTTFNEIDPVDCDGIYLSS 1440
            TEDSKSVLHVKYIAALSRELAENHSTSESAHNVSMPVESLQPTTFNEIDPVDCDGIYLSS
Sbjct: 1381 TEDSKSVLHVKYIAALSRELAENHSTSESAHNVSMPVESLQPTTFNEIDPVDCDGIYLSS 1440

Query: 1441 CGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAFPREF 1500
            CGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAFPREF
Sbjct: 1441 CGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAFPREF 1500

Query: 1501 QKFGNPSMSSIGSLSHVSGQSNKSTEEVNPLYVQKAVAFLQSTAKAVGKNKVLKDISVHR 1560
            QKFGNPSMSSIGSLSHVSGQSNKSTEEVNPLYVQKAVAFLQSTAKAVGKNKVLKDISVHR
Sbjct: 1501 QKFGNPSMSSIGSLSHVSGQSNKSTEEVNPLYVQKAVAFLQSTAKAVGKNKVLKDISVHR 1560

Query: 1561 HRKVSRNLEAVSLVLSKLYFSGKQDKSISCTRVNPSILMWDTLKYSLVSMEIAARSKTDV 1620
            HRKVSRNLEAVSLVLSKLYFSGKQDKSISCTRVNPSILMWDTLKYSLVSMEIAARSKTDV
Sbjct: 1561 HRKVSRNLEAVSLVLSKLYFSGKQDKSISCTRVNPSILMWDTLKYSLVSMEIAARSKTDV 1620

Query: 1621 NPNIGLNTLYKELKTSGGFILSLLLKVIQSIKCEDSLLLLQRFCGIQRFADSICSGVSNE 1680
            NPNIGLNTLYKELKTSGGFILSLLLKVIQSIKCEDSLLLLQRFCGIQRFADSICSGVSNE
Sbjct: 1621 NPNIGLNTLYKELKTSGGFILSLLLKVIQSIKCEDSLLLLQRFCGIQRFADSICSGVSNE 1680

Query: 1681 NANDSCGQGILHILTSLRVELPQFDIQFLSRGSDPVLAHDPFASVMWVLFCLPCPFLSCK 1740
            NANDSCGQGILHILTSLRVELPQFDIQFLSRGSDPVLAHDPFASVMWVLFCLPCPFLSCK
Sbjct: 1681 NANDSCGQGILHILTSLRVELPQFDIQFLSRGSDPVLAHDPFASVMWVLFCLPCPFLSCK 1740

Query: 1741 ESLISLVHIFYLVSVAQGIIASCMKYQSKIDGMGFSDCLIADICKIMGESEYARRYFVSD 1800
            ESLISLVHIFYLVSVAQGIIASCMKYQSKIDGMGFSDCLIADICKIMGESEYARRYFVSD
Sbjct: 1741 ESLISLVHIFYLVSVAQGIIASCMKYQSKIDGMGFSDCLIADICKIMGESEYARRYFVSD 1800

Query: 1801 YTEPSCNVKDMIRSLTFTYLRRCALLLKLLNSSARAPIFDGETALERYLAVDDMIDNIAL 1860
            YTEPSCNVKDMIRSLTFTYLRRCALLLKLLNSSARAPIFDGETALERYLAVDDMIDNIAL
Sbjct: 1801 YTEPSCNVKDMIRSLTFTYLRRCALLLKLLNSSARAPIFDGETALERYLAVDDMIDNIAL 1860

Query: 1861 ELNEIGKLEKIFKIPPLDVVLKDEASRSLVWKWFCHFNREFEFQRSKNLKHCTPAVPFQL 1920
            ELNEIGKLEKIFKIPPLDVVLKDEASRSLVWKWFCHFNREFEFQRSKNLKHCTPAVPFQL
Sbjct: 1861 ELNEIGKLEKIFKIPPLDVVLKDEASRSLVWKWFCHFNREFEFQRSKNLKHCTPAVPFQL 1920

Query: 1921 IRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANICA 1980
            IRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANICA
Sbjct: 1921 IRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANICA 1980

Query: 1981 AGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYMV 2040
            AGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYMV
Sbjct: 1981 AGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYMV 2037

BLAST of MS019737 vs. NCBI nr
Match: XP_038903782.1 (E3 ubiquitin-protein ligase PRT6 isoform X1 [Benincasa hispida] >XP_038903783.1 E3 ubiquitin-protein ligase PRT6 isoform X1 [Benincasa hispida])

HSP 1 Score: 3569.6 bits (9255), Expect = 0.0e+00
Identity = 1791/2042 (87.71%), Postives = 1900/2042 (93.05%), Query Frame = 0

Query: 1    MDDMDIGSPSESAPLKPRDRIVRRLALLGVPEELLDQLQPGLVSFVKDNKILIPQLVSAI 60
            MDDMDIGSPSESAPLKPRDRI+RRLA+LGVP+ELLDQL  GLVSFVKDNK LIPQLV+AI
Sbjct: 1    MDDMDIGSPSESAPLKPRDRILRRLAVLGVPDELLDQLFRGLVSFVKDNKFLIPQLVTAI 60

Query: 61   LPTDVEVAEVIRDAKAGSKKSMAGPTMKNQFRESMVWLQWLMFEGEPTFALKNLSKMSFG 120
            LP DVEV EVIRDAKAGSKKS+ GPTMK QFRESM+WLQWLMFEGEP +ALKNLSKMS G
Sbjct: 61   LPADVEVVEVIRDAKAGSKKSLTGPTMKAQFRESMMWLQWLMFEGEPAYALKNLSKMSVG 120

Query: 121  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVT 180
            QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNH+DHDYSIIYTGGGCCDCGD T
Sbjct: 121  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHQDHDYSIIYTGGGCCDCGDAT 180

Query: 181  AWKREGFCSKHKGAERIQPLPEEFVESVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 240
            AWKREGFCSKHKGAE+IQPLPEE V+SVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV
Sbjct: 181  AWKREGFCSKHKGAEQIQPLPEELVKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 240

Query: 241  MEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISLAGFLDILVRLERLLTDIVVKK 300
             EHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVIS AG LDILVRLERLLTD VVKK
Sbjct: 241  TEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGLLDILVRLERLLTDSVVKK 300

Query: 301  VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHALKKYPLLPTFSVQIFTVPT 360
            VHELLLKLLGEPIFKYEFAKVFLNYYPTVI+EAIE+ SDH LKKYPLLPTFSVQIFTVPT
Sbjct: 301  VHELLLKLLGEPIFKYEFAKVFLNYYPTVINEAIEDSSDHVLKKYPLLPTFSVQIFTVPT 360

Query: 361  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAV 420
            LTPRLV EMNLLSILLGCLEDIFISCVSEDGRLQV+KWSNLYETTIRVVED+RFVMSHAV
Sbjct: 361  LTPRLVVEMNLLSILLGCLEDIFISCVSEDGRLQVVKWSNLYETTIRVVEDVRFVMSHAV 420

Query: 421  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHTL 480
            VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIH+L
Sbjct: 421  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL 480

Query: 481  LVNEAFSAASSSSSSEETADAMSFHAFKQNPDDMDGIRHAKVGRLSQESAACNVFGRSST 540
            LV EAFSAA  SSS E+ ADAM F  +KQN DD+D +RHAKVGRLSQESAACNV G++ST
Sbjct: 481  LVKEAFSAA--SSSCEDAADAMYFQTYKQNVDDIDSVRHAKVGRLSQESAACNVLGKNST 540

Query: 541  VTSASRVDEVCSDTISSTIMWLTYECLKVIDSWLGTENVLGGFPNMFAENASMASSCKVY 600
             TSASRVDEVCSD ISSTIMWLTYECLK+IDSWLGTENV G  PNM  E+ S+  SCK Y
Sbjct: 541  STSASRVDEVCSDAISSTIMWLTYECLKIIDSWLGTENVSGSIPNMLDESISLTPSCKFY 600

Query: 601  SLRKPSALESRKISSKVEKGKFIFEKVAGKSKDHNRQYSSRIYSGLQMSIDNEHGVSLGE 660
            S RK SALES+KI  K+EKGKF+FEK+  +SK HNRQYSSR+Y GLQMSIDNEHG+ LGE
Sbjct: 601  SSRKMSALESKKIPYKMEKGKFVFEKLPRRSKYHNRQYSSRMYGGLQMSIDNEHGIGLGE 660

Query: 661  DNQLMDVTNDTVTVEDYSMETDALHFLSFSAWPNIVYDVSSQDISIHIPLHRLLSLLLQK 720
            D+QLMDVTNDTVT EDY+ME DALHFLS SAWPNIVYDVSSQDISIHIPLHRLLSLLLQK
Sbjct: 661  DHQLMDVTNDTVTDEDYAMEIDALHFLSLSAWPNIVYDVSSQDISIHIPLHRLLSLLLQK 720

Query: 721  ALRSSFSESVVPSATGASSSNLSFEYVDFFKSVLTDYHPYGFSAFVMEHPLRIKVFCAEV 780
            AL S F+ESV+PSATGASSSNLS EYVDFFKS+LTD HPYGFS+FVMEHPLRIKVFCAEV
Sbjct: 721  ALGSCFNESVLPSATGASSSNLSSEYVDFFKSILTDCHPYGFSSFVMEHPLRIKVFCAEV 780

Query: 781  NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPELYISRILERFRLLD 840
            NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPP+LY+SRI+ERFRL D
Sbjct: 781  NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVSRIIERFRLSD 840

Query: 841  YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQL 900
            YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLT+AESLKRELIYKLAIGDATHSQL
Sbjct: 841  YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTIAESLKRELIYKLAIGDATHSQL 900

Query: 901  VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLRWKYWKELDLYHPRWSLRDLQIA 960
            VKALPRDL KCHQLQEILDTIAVYSNPSGFNQGMYSL WKYWKELDLYHPRWSLRDLQ+A
Sbjct: 901  VKALPRDLCKCHQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPRWSLRDLQVA 960

Query: 961  EERYLRSCGASALTSQLPKWTKIYPPFRGLARIATCKAVLQIIRAVLFYSVFTDKSTKSR 1020
            EERYLRSCG SALTSQLPKWT IYPPFRGLARIATCK VLQ IRAVLFYSVF + STKSR
Sbjct: 961  EERYLRSCGVSALTSQLPKWTNIYPPFRGLARIATCKTVLQFIRAVLFYSVFAEISTKSR 1020

Query: 1021 APDSVLLTALHLLALALDICFQRKESSDQSFCSEDSIPLLLFADEEVDEGLTYGLGRQSL 1080
            APDSVLL+ALHLLALALDIC Q+KESSDQSF ++DSIPLLLFA EE+DEGL YG GRQSL
Sbjct: 1021 APDSVLLSALHLLALALDICLQQKESSDQSFYAQDSIPLLLFAAEEIDEGLAYGFGRQSL 1080

Query: 1081 LSLLILLMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDSRCMGKIRQHAPEILGYL 1140
            LSLL+LLMKMH+KEGRENLLEAGSCNLSSLVESLLKKFSEIDS CMGK++Q APEILGYL
Sbjct: 1081 LSLLVLLMKMHRKEGRENLLEAGSCNLSSLVESLLKKFSEIDSHCMGKVQQLAPEILGYL 1140

Query: 1141 SQSLPSSDPNSLSETSDSDKLKAKARERQAAILEKMRAEQYKFLASVDSSV--EDDAEFG 1200
            SQS+P+S+ N  S TSDS+K KAKARERQAAILEKMRAEQ KFLASVD+SV  +DD E G
Sbjct: 1141 SQSVPTSNTNRPSGTSDSEKRKAKARERQAAILEKMRAEQSKFLASVDASVNDDDDREIG 1200

Query: 1201 QESEKPSVSDSAEQSETVCSLCHDSSSSVPISYLILLQKSKLVSLIDRGTVSWDQPYWRD 1260
            QESE P+VSD AEQSETVCSLCHDSSSSVPIS+LILLQKSKLVSLIDRG VSWDQPYWRD
Sbjct: 1201 QESENPNVSDIAEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGPVSWDQPYWRD 1260

Query: 1261 EHASSIPRRNLDQSGLSTSSGGSGGISSPQLAELIQNAVKEYASHGLPGEVGAFLDFVKS 1320
            EHAS++ +R+LDQSGLS SS GSG ISSPQLAELIQNAVKEY++HGLPGEVGAFL+FVKS
Sbjct: 1261 EHASTVSKRDLDQSGLSPSSAGSGVISSPQLAELIQNAVKEYSNHGLPGEVGAFLEFVKS 1320

Query: 1321 HFPPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHENMHSKLNEDENFSKVASS 1380
            HFPPLR+IQVPGTSNV+GEKIIFSFD LEEDIYLSVRKEMH+ +HSK NED+  S+VAS 
Sbjct: 1321 HFPPLRDIQVPGTSNVRGEKIIFSFDKLEEDIYLSVRKEMHDTLHSKFNEDDKASEVASG 1380

Query: 1381 LVTEDSKSVLHVKYIAALSRELAENHSTSESAHNVSMPVESLQPTTFNEIDPVDCDGIYL 1440
               EDSKSVLHVKYIAALSRELAENHSTSES+HN+  PVESLQPTTFNEI P DCDGIYL
Sbjct: 1381 ---EDSKSVLHVKYIAALSRELAENHSTSESSHNIHTPVESLQPTTFNEIGPTDCDGIYL 1440

Query: 1441 SSCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAFPR 1500
            SSCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAFPR
Sbjct: 1441 SSCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAFPR 1500

Query: 1501 EFQKFGNPSMSSIGSLSHVSGQSNKSTEEVNPLYVQKAVAFLQSTAKAVGKNKVLKDISV 1560
            E QK  NP+ SS+GS SHVSG  NKS+E+VNPLY+Q+AVA LQ  A AVGKNKVLKDISV
Sbjct: 1501 ELQKIWNPATSSVGSSSHVSGHLNKSSEKVNPLYIQQAVALLQYAANAVGKNKVLKDISV 1560

Query: 1561 HRHRKVSRNLEAVSLVLSKLYFSGKQDKSISCTRVNPSILMWDTLKYSLVSMEIAARSKT 1620
            HRHRKVSRNLEAVSLVLSKLYFSGKQDK IS +RVNPSILMWDTLKYSL+SMEIAARSKT
Sbjct: 1561 HRHRKVSRNLEAVSLVLSKLYFSGKQDKLISSSRVNPSILMWDTLKYSLISMEIAARSKT 1620

Query: 1621 DVNPNIGLNTLYKELKTSGGFILSLLLKVIQSIKCEDSLLLLQRFCGIQRFADSICSGVS 1680
            D+NPNIG+NTLYKELK SGGF+LSLLLKVIQS+KCEDSLLLLQRFCGIQRFADSICSG S
Sbjct: 1621 DMNPNIGINTLYKELKISGGFVLSLLLKVIQSVKCEDSLLLLQRFCGIQRFADSICSGTS 1680

Query: 1681 NENANDSCGQGILHILTSLRVELPQFDIQFLSRGSDPVLAHDPFASVMWVLFCLPCPFLS 1740
            NENA+DSCG+GILHILTSL VELPQFDIQFLSRGSDPVLAHDPFAS+MWV+FCLP PFLS
Sbjct: 1681 NENASDSCGRGILHILTSLHVELPQFDIQFLSRGSDPVLAHDPFASLMWVMFCLPFPFLS 1740

Query: 1741 CKESLISLVHIFYLVSVAQGIIASCMKYQSKIDGMGFSDCLIADICKIMGESEYARRYFV 1800
            C+ESL+SLVHIFYLVSVAQ II S +K Q K+DG+G SDCLIADICKIMGESE AR YFV
Sbjct: 1741 CRESLLSLVHIFYLVSVAQAIITSFIKSQCKVDGLGSSDCLIADICKIMGESECARLYFV 1800

Query: 1801 SDYTEPSCNVKDMIRSLTFTYLRRCALLLKLLNSSARAPIFDGETALERYLAVDDMIDNI 1860
            S+YTEPSCNVKDMIRSLTF YLRRCALLLKLL+SSAR PIF GETALE YLA +++IDNI
Sbjct: 1801 SNYTEPSCNVKDMIRSLTFPYLRRCALLLKLLSSSARVPIFYGETALETYLAGNNIIDNI 1860

Query: 1861 ALELNEIGKLEKIFKIPPLDVVLKDEASRSLVWKWFCHFNREFEFQRSKNLKHCTPAVPF 1920
             +ELNEI KLEK+F+IPPLD VLKD  SR  V KWFCHF++EFEFQRSK +KHCTPAVPF
Sbjct: 1861 TVELNEIEKLEKMFEIPPLDTVLKDGTSRLFVSKWFCHFDKEFEFQRSKIIKHCTPAVPF 1920

Query: 1921 QLIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANI 1980
            QLIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLC+PSWKSCCRESGCQAHAN+
Sbjct: 1921 QLIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCTPSWKSCCRESGCQAHANV 1980

Query: 1981 CAAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSY 2040
            CAAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEED EMRRGKPLYLNEERYAALSY
Sbjct: 1981 CAAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDTEMRRGKPLYLNEERYAALSY 2037

BLAST of MS019737 vs. NCBI nr
Match: XP_008442546.1 (PREDICTED: E3 ubiquitin-protein ligase PRT6 isoform X1 [Cucumis melo] >XP_008442547.1 PREDICTED: E3 ubiquitin-protein ligase PRT6 isoform X1 [Cucumis melo])

HSP 1 Score: 3540.0 bits (9178), Expect = 0.0e+00
Identity = 1777/2041 (87.07%), Postives = 1888/2041 (92.50%), Query Frame = 0

Query: 1    MDDMDIGSPSESAPLKPRDRIVRRLALLGVPEELLDQLQPGLVSFVKDNKILIPQLVSAI 60
            MDDMDIGSPSES PLKPRDRI+RRLA+LGVP+ELLDQL  GLV FVKDNK LIPQLVSAI
Sbjct: 1    MDDMDIGSPSESTPLKPRDRILRRLAVLGVPDELLDQLFRGLVIFVKDNKFLIPQLVSAI 60

Query: 61   LPTDVEVAEVIRDAKAGSKKSMAGPTMKNQFRESMVWLQWLMFEGEPTFALKNLSKMSFG 120
            LPTDVEV EVIRDA  GSKK++ GPTMK  FRESM+WLQWLMFEGEP +ALKNLSKMS G
Sbjct: 61   LPTDVEVVEVIRDAIPGSKKTLTGPTMKAHFRESMMWLQWLMFEGEPAYALKNLSKMSVG 120

Query: 121  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVT 180
            QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNH+DHDYSIIYTGGGCCDCGD T
Sbjct: 121  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHQDHDYSIIYTGGGCCDCGDAT 180

Query: 181  AWKREGFCSKHKGAERIQPLPEEFVESVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 240
            AWKREGFCSKHKGAE+IQPLPEE+V+SVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV
Sbjct: 181  AWKREGFCSKHKGAEQIQPLPEEYVKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 240

Query: 241  MEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISLAGFLDILVRLERLLTDIVVKK 300
             EHKKVANELTFAVVEMLLDFCK+SESLLSFVSKRVIS AG LDILVRLERLLTD VVKK
Sbjct: 241  TEHKKVANELTFAVVEMLLDFCKHSESLLSFVSKRVISSAGLLDILVRLERLLTDSVVKK 300

Query: 301  VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHALKKYPLLPTFSVQIFTVPT 360
            VHELLLKLLGEP+FKYEFAKVFLNYYPTVISEAIE+ SDHALKKYPLLPTFSVQIFTVPT
Sbjct: 301  VHELLLKLLGEPVFKYEFAKVFLNYYPTVISEAIEDSSDHALKKYPLLPTFSVQIFTVPT 360

Query: 361  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAV 420
            LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQV+KWSNLYETTIRVVED+RFVMSHAV
Sbjct: 361  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVVKWSNLYETTIRVVEDVRFVMSHAV 420

Query: 421  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHTL 480
            VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIH+L
Sbjct: 421  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL 480

Query: 481  LVNEAFSAASSSSSSEETADAMSFHAFKQNPDDMDGIRHAKVGRLSQESAACNVFGRSST 540
            LV EAFSAA  SSS E+TAD M F  +KQN DD+D +RHAKVGRLSQESAAC V G+SST
Sbjct: 481  LVKEAFSAA--SSSCEDTADGMYFQTYKQNVDDIDSVRHAKVGRLSQESAACGVLGKSST 540

Query: 541  VTSASRVDEVCSDTISSTIMWLTYECLKVIDSWLGTENVLGGFPNMFAENASMASSCKVY 600
             TSASRVDEVCSD ISSTIMWLTYECLK+ID+WLGTEN+ G  PNM  E+ S A SCK Y
Sbjct: 541  STSASRVDEVCSDAISSTIMWLTYECLKIIDNWLGTENISGSIPNMLGESISPAPSCKFY 600

Query: 601  SLRKPSALESRKISSKVEKGKFIFEKVAGKSKDHNRQYSSRIYSGLQMSIDNEHGVSLGE 660
            S RK SAL S+K+S K+EKGKFIF+K++ +SK HNRQYSSR+YSGLQMSIDNEHG+SLGE
Sbjct: 601  SSRKTSALASKKMSYKMEKGKFIFDKLSRRSKYHNRQYSSRMYSGLQMSIDNEHGISLGE 660

Query: 661  DNQLMDVTNDTVTVEDYSMETDALHFLSFSAWPNIVYDVSSQDISIHIPLHRLLSLLLQK 720
            DN LMDVTNDTVT EDY+ME DALHFLS S+WPNIVYDVSSQDISIHIPLHRLLSLLLQK
Sbjct: 661  DNHLMDVTNDTVTDEDYAMEIDALHFLSLSSWPNIVYDVSSQDISIHIPLHRLLSLLLQK 720

Query: 721  ALRSSFSESVVPSATGASSSNLSFEYVDFFKSVLTDYHPYGFSAFVMEHPLRIKVFCAEV 780
            AL S FSES VPSATGASSSNLS EYVDFFKSVLTD HPYGFS+FVMEHPLRIKVFCAEV
Sbjct: 721  ALSSCFSESGVPSATGASSSNLSSEYVDFFKSVLTDCHPYGFSSFVMEHPLRIKVFCAEV 780

Query: 781  NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPELYISRILERFRLLD 840
            NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPP+LY+ RILERFRL D
Sbjct: 781  NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVCRILERFRLSD 840

Query: 841  YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQL 900
            YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLT+AESLKRELIYKLAIGDATHSQL
Sbjct: 841  YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTIAESLKRELIYKLAIGDATHSQL 900

Query: 901  VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLRWKYWKELDLYHPRWSLRDLQIA 960
            VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSL WKYWKELDLYHPRWSLRDLQ+A
Sbjct: 901  VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPRWSLRDLQVA 960

Query: 961  EERYLRSCGASALTSQLPKWTKIYPPFRGLARIATCKAVLQIIRAVLFYSVFTDKSTKSR 1020
            EERYLRSCG SALTSQLPKWTKIYPPFRGLARIATCK VLQ IRAVLFYSVF + STKSR
Sbjct: 961  EERYLRSCGVSALTSQLPKWTKIYPPFRGLARIATCKTVLQFIRAVLFYSVFAEISTKSR 1020

Query: 1021 APDSVLLTALHLLALALDICFQRKESSDQSFCSEDSIPLLLFADEEVDEGLTYGLGRQSL 1080
            APDSVLL+ALHLLALALD+CFQ+KES DQSF + DSIPLLLFA EE++EGL YG GRQSL
Sbjct: 1021 APDSVLLSALHLLALALDVCFQQKESRDQSFDAPDSIPLLLFAAEEIEEGLAYGFGRQSL 1080

Query: 1081 LSLLILLMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDSRCMGKIRQHAPEILGYL 1140
            LSLLILLMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDS CMGK++Q APEILGYL
Sbjct: 1081 LSLLILLMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDSHCMGKVQQLAPEILGYL 1140

Query: 1141 SQSLPSSDPNSLSETSDSDKLKAKARERQAAILEKMRAEQYKFLASVDSSV-EDDAEFGQ 1200
            SQS+P+S  +  +ETSDS+K KAKARERQAAILEKMRAEQ KFLASVD+SV +DD EFGQ
Sbjct: 1141 SQSVPTSTTSRPAETSDSEKRKAKARERQAAILEKMRAEQSKFLASVDASVDDDDTEFGQ 1200

Query: 1201 ESEKPSVSDSAEQSETVCSLCHDSSSSVPISYLILLQKSKLVSLIDRGTVSWDQPYWRDE 1260
            E EKP+VSD AEQSETVCSLCHDSSSSVPIS+LILLQKSKLVSLIDRG VSWDQPYWRDE
Sbjct: 1201 EPEKPNVSDDAEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGPVSWDQPYWRDE 1260

Query: 1261 HASSIPRRNLDQSGLSTSSGGSGGISSPQLAELIQNAVKEYASHGLPGEVGAFLDFVKSH 1320
            HAS+  +R++DQSG+STSS GS  ISSPQLAELIQNAVKEY +HGLPGEVGAFLDF+KSH
Sbjct: 1261 HASTTSKRDIDQSGVSTSSAGSVVISSPQLAELIQNAVKEYTNHGLPGEVGAFLDFLKSH 1320

Query: 1321 FPPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHENMHSKLNEDENFSKVASSL 1380
            FPPLR+IQVPGTSNVKGEKIIFSFDTLEEDIYLSV KEMH+ +HSK +E E  S VAS  
Sbjct: 1321 FPPLRDIQVPGTSNVKGEKIIFSFDTLEEDIYLSVFKEMHDTLHSKFHEGEKISTVASG- 1380

Query: 1381 VTEDSKSVLHVKYIAALSRELAENHSTSESAHNVSMPVESLQPTTFNEIDPVDCDGIYLS 1440
              EDS+SVLHVKYIAALSRELAEN STSESA NV MPVES QPT  NEI P+DCDGIYLS
Sbjct: 1381 --EDSRSVLHVKYIAALSRELAENRSTSESARNVHMPVESSQPTILNEIGPIDCDGIYLS 1440

Query: 1441 SCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAFPRE 1500
            SCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRL+NSTLPAFPRE
Sbjct: 1441 SCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLSNSTLPAFPRE 1500

Query: 1501 FQKFGNPSMSSIGSLSHVSGQSNKSTEEVNPLYVQKAVAFLQSTAKAVGKNKVLKDISVH 1560
            FQK  NP  SS+G+LSHVSG  NKS E VNPLY+Q+AVA LQS AKAVGKN VLKDISVH
Sbjct: 1501 FQKIWNPRTSSVGTLSHVSGHLNKSNERVNPLYIQEAVALLQSAAKAVGKNNVLKDISVH 1560

Query: 1561 RHRKVSRNLEAVSLVLSKLYFSGKQDKSISCTRVNPSILMWDTLKYSLVSMEIAARSKTD 1620
            RH+KVSRNLEAVSLVLSKLYFS KQDK IS +RVNPSILMWDTLKYSLVSMEIAARSKTD
Sbjct: 1561 RHKKVSRNLEAVSLVLSKLYFSWKQDKLISSSRVNPSILMWDTLKYSLVSMEIAARSKTD 1620

Query: 1621 VNPNIGLNTLYKELKTSGGFILSLLLKVIQSIKCEDSLLLLQRFCGIQRFADSICSGVSN 1680
            +NP+IGLNTLYKELKTSGGF+LSLLLKVI S+KCEDSLLLLQR CGIQRFADSICSG+SN
Sbjct: 1621 MNPSIGLNTLYKELKTSGGFVLSLLLKVIHSVKCEDSLLLLQRLCGIQRFADSICSGMSN 1680

Query: 1681 ENANDSCGQGILHILTSLRVELPQFDIQFLSRGSDPVLAHDPFASVMWVLFCLPCPFLSC 1740
            ENA+DSCG+GILHILTSLR ELP FD QFLSRGSDPV+AHDPFAS+MWVLFCLP PFLSC
Sbjct: 1681 ENASDSCGRGILHILTSLRSELPHFDSQFLSRGSDPVIAHDPFASLMWVLFCLPFPFLSC 1740

Query: 1741 KESLISLVHIFYLVSVAQGIIASCMKYQSKIDGMGFSDCLIADICKIMGESEYARRYFVS 1800
            +ESL+SLVHIFYLVSV Q II    K Q K+DG+G SDCLI DICKIMGESEYAR+YFVS
Sbjct: 1741 RESLLSLVHIFYLVSVTQAIITYFTKSQCKVDGLGSSDCLITDICKIMGESEYARQYFVS 1800

Query: 1801 DYTEPSCNVKDMIRSLTFTYLRRCALLLKLLNSSARAPIFDGETALERYLAVDDMIDNIA 1860
            +YTEPSCN+KD +RSLTF +LRRCALLL+LL+SSAR PIFDGETALE YL  ++MIDNI 
Sbjct: 1801 NYTEPSCNIKDTVRSLTFPFLRRCALLLQLLSSSARVPIFDGETALETYLVGNNMIDNIT 1860

Query: 1861 LELNEIGKLEKIFKIPPLDVVLKDEASRSLVWKWFCHFNREFEFQRSKNLKHCTPAVPFQ 1920
            +ELNE+ KL+K+F IPPLD+VLKD  SR LV KWFCHFN+EFEFQRSK +KHCTPAVPFQ
Sbjct: 1861 VELNEVEKLQKMFDIPPLDIVLKDGTSRLLVSKWFCHFNKEFEFQRSKIIKHCTPAVPFQ 1920

Query: 1921 LIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANIC 1980
            LIRLPHVYHDLLQRYIKKRC DCKHVIDDPALCL+CG+LCSPSWKSCCRESGCQAHANIC
Sbjct: 1921 LIRLPHVYHDLLQRYIKKRCTDCKHVIDDPALCLICGKLCSPSWKSCCRESGCQAHANIC 1980

Query: 1981 AAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYM 2040
            AAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYM
Sbjct: 1981 AAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYM 2036

BLAST of MS019737 vs. NCBI nr
Match: KAA0044092.1 (E3 ubiquitin-protein ligase PRT6 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 3540.0 bits (9178), Expect = 0.0e+00
Identity = 1777/2041 (87.07%), Postives = 1888/2041 (92.50%), Query Frame = 0

Query: 1    MDDMDIGSPSESAPLKPRDRIVRRLALLGVPEELLDQLQPGLVSFVKDNKILIPQLVSAI 60
            MDDMDIGSPSES PLKPRDRI+RRLA+LGVP+ELLDQL  GLV FVKDNK LIPQLVSAI
Sbjct: 1    MDDMDIGSPSESTPLKPRDRILRRLAVLGVPDELLDQLFRGLVIFVKDNKFLIPQLVSAI 60

Query: 61   LPTDVEVAEVIRDAKAGSKKSMAGPTMKNQFRESMVWLQWLMFEGEPTFALKNLSKMSFG 120
            LPTDVEV EVIRDA  GSKK++ GPTMK  FRESM+WLQWLMFEGEP +ALKNLSKMS G
Sbjct: 61   LPTDVEVVEVIRDAIPGSKKTLTGPTMKAHFRESMMWLQWLMFEGEPAYALKNLSKMSVG 120

Query: 121  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVT 180
            QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNH+DHDYSIIYTGGGCCDCGD T
Sbjct: 121  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHQDHDYSIIYTGGGCCDCGDAT 180

Query: 181  AWKREGFCSKHKGAERIQPLPEEFVESVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 240
            AWKREGFCSKHKGAE+IQPLPEE+V+SVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV
Sbjct: 181  AWKREGFCSKHKGAEQIQPLPEEYVKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 240

Query: 241  MEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISLAGFLDILVRLERLLTDIVVKK 300
             EHKKVANELTFAVVEMLLDFCK+SESLLSFVSKRVIS AG LDILVRLERLLTD VVKK
Sbjct: 241  TEHKKVANELTFAVVEMLLDFCKHSESLLSFVSKRVISSAGLLDILVRLERLLTDSVVKK 300

Query: 301  VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHALKKYPLLPTFSVQIFTVPT 360
            VHELLLKLLGEP+FKYEFAKVFLNYYPTVISEAIE+ SDHALKKYPLLPTFSVQIFTVPT
Sbjct: 301  VHELLLKLLGEPVFKYEFAKVFLNYYPTVISEAIEDSSDHALKKYPLLPTFSVQIFTVPT 360

Query: 361  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAV 420
            LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQV+KWSNLYETTIRVVED+RFVMSHAV
Sbjct: 361  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVVKWSNLYETTIRVVEDVRFVMSHAV 420

Query: 421  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHTL 480
            VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIH+L
Sbjct: 421  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL 480

Query: 481  LVNEAFSAASSSSSSEETADAMSFHAFKQNPDDMDGIRHAKVGRLSQESAACNVFGRSST 540
            LV EAFSAA  SSS E+TAD M F  +KQN DD+D +RHAKVGRLSQESAAC V G+SST
Sbjct: 481  LVKEAFSAA--SSSCEDTADGMYFQTYKQNVDDIDSVRHAKVGRLSQESAACGVLGKSST 540

Query: 541  VTSASRVDEVCSDTISSTIMWLTYECLKVIDSWLGTENVLGGFPNMFAENASMASSCKVY 600
             TSASRVDEVCSD ISSTIMWLTYECLK+ID+WLGTEN+ G  PNM  E+ S A SCK Y
Sbjct: 541  STSASRVDEVCSDAISSTIMWLTYECLKIIDNWLGTENISGSIPNMLGESISPAPSCKFY 600

Query: 601  SLRKPSALESRKISSKVEKGKFIFEKVAGKSKDHNRQYSSRIYSGLQMSIDNEHGVSLGE 660
            S RK SAL S+K+S K+EKGKFIF+K++ +SK HNRQYSSR+YSGLQMSIDNEHG+SLGE
Sbjct: 601  SSRKTSALASKKMSYKMEKGKFIFDKLSRRSKYHNRQYSSRMYSGLQMSIDNEHGISLGE 660

Query: 661  DNQLMDVTNDTVTVEDYSMETDALHFLSFSAWPNIVYDVSSQDISIHIPLHRLLSLLLQK 720
            DN LMDVTNDTVT EDY+ME DALHFLS S+WPNIVYDVSSQDISIHIPLHRLLSLLLQK
Sbjct: 661  DNHLMDVTNDTVTDEDYAMEIDALHFLSLSSWPNIVYDVSSQDISIHIPLHRLLSLLLQK 720

Query: 721  ALRSSFSESVVPSATGASSSNLSFEYVDFFKSVLTDYHPYGFSAFVMEHPLRIKVFCAEV 780
            AL S FSES VPSATGASSSNLS EYVDFFKSVLTD HPYGFS+FVMEHPLRIKVFCAEV
Sbjct: 721  ALSSCFSESGVPSATGASSSNLSSEYVDFFKSVLTDCHPYGFSSFVMEHPLRIKVFCAEV 780

Query: 781  NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPELYISRILERFRLLD 840
            NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPP+LY+ RILERFRL D
Sbjct: 781  NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVCRILERFRLSD 840

Query: 841  YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQL 900
            YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLT+AESLKRELIYKLAIGDATHSQL
Sbjct: 841  YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTIAESLKRELIYKLAIGDATHSQL 900

Query: 901  VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLRWKYWKELDLYHPRWSLRDLQIA 960
            VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSL WKYWKELDLYHPRWSLRDLQ+A
Sbjct: 901  VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPRWSLRDLQVA 960

Query: 961  EERYLRSCGASALTSQLPKWTKIYPPFRGLARIATCKAVLQIIRAVLFYSVFTDKSTKSR 1020
            EERYLRSCG SALTSQLPKWTKIYPPFRGLARIATCK VLQ IRAVLFYSVF + STKSR
Sbjct: 961  EERYLRSCGVSALTSQLPKWTKIYPPFRGLARIATCKTVLQFIRAVLFYSVFAEISTKSR 1020

Query: 1021 APDSVLLTALHLLALALDICFQRKESSDQSFCSEDSIPLLLFADEEVDEGLTYGLGRQSL 1080
            APDSVLL+ALHLLALALD+CFQ+KES DQSF + DSIPLLLFA EE++EGL YG GRQSL
Sbjct: 1021 APDSVLLSALHLLALALDVCFQQKESRDQSFDAPDSIPLLLFAAEEIEEGLAYGFGRQSL 1080

Query: 1081 LSLLILLMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDSRCMGKIRQHAPEILGYL 1140
            LSLLILLMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDS CMGK++Q APEILGYL
Sbjct: 1081 LSLLILLMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDSHCMGKVQQLAPEILGYL 1140

Query: 1141 SQSLPSSDPNSLSETSDSDKLKAKARERQAAILEKMRAEQYKFLASVDSSV-EDDAEFGQ 1200
            SQS+P+S  +  +ETSDS+K KAKARERQAAILEKMRAEQ KFLASVD+SV +DD EFGQ
Sbjct: 1141 SQSVPTSTTSRPAETSDSEKRKAKARERQAAILEKMRAEQSKFLASVDASVDDDDTEFGQ 1200

Query: 1201 ESEKPSVSDSAEQSETVCSLCHDSSSSVPISYLILLQKSKLVSLIDRGTVSWDQPYWRDE 1260
            E EKP+VSD AEQSETVCSLCHDSSSSVPIS+LILLQKSKLVSLIDRG VSWDQPYWRDE
Sbjct: 1201 EPEKPNVSDDAEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGPVSWDQPYWRDE 1260

Query: 1261 HASSIPRRNLDQSGLSTSSGGSGGISSPQLAELIQNAVKEYASHGLPGEVGAFLDFVKSH 1320
            HAS+  +R++DQSG+STSS GS  ISSPQLAELIQNAVKEY +HGLPGEVGAFLDF+KSH
Sbjct: 1261 HASTTSKRDIDQSGVSTSSAGSVVISSPQLAELIQNAVKEYTNHGLPGEVGAFLDFLKSH 1320

Query: 1321 FPPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHENMHSKLNEDENFSKVASSL 1380
            FPPLR+IQVPGTSNVKGEKIIFSFDTLEEDIYLSV KEMH+ +HSK +E E  S VAS  
Sbjct: 1321 FPPLRDIQVPGTSNVKGEKIIFSFDTLEEDIYLSVFKEMHDTLHSKFHEGEKISTVASG- 1380

Query: 1381 VTEDSKSVLHVKYIAALSRELAENHSTSESAHNVSMPVESLQPTTFNEIDPVDCDGIYLS 1440
              EDS+SVLHVKYIAALSRELAEN STSESA NV MPVES QPT  NEI P+DCDGIYLS
Sbjct: 1381 --EDSRSVLHVKYIAALSRELAENRSTSESARNVHMPVESSQPTILNEIGPIDCDGIYLS 1440

Query: 1441 SCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAFPRE 1500
            SCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRL+NSTLPAFPRE
Sbjct: 1441 SCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLSNSTLPAFPRE 1500

Query: 1501 FQKFGNPSMSSIGSLSHVSGQSNKSTEEVNPLYVQKAVAFLQSTAKAVGKNKVLKDISVH 1560
            FQK  NP  SS+G+LSHVSG  NKS E VNPLY+Q+AVA LQS AKAVGKN VLKDISVH
Sbjct: 1501 FQKIWNPRTSSVGTLSHVSGHLNKSNERVNPLYIQEAVALLQSAAKAVGKNNVLKDISVH 1560

Query: 1561 RHRKVSRNLEAVSLVLSKLYFSGKQDKSISCTRVNPSILMWDTLKYSLVSMEIAARSKTD 1620
            RH+KVSRNLEAVSLVLSKLYFS KQDK IS +RVNPSILMWDTLKYSLVSMEIAARSKTD
Sbjct: 1561 RHKKVSRNLEAVSLVLSKLYFSWKQDKLISSSRVNPSILMWDTLKYSLVSMEIAARSKTD 1620

Query: 1621 VNPNIGLNTLYKELKTSGGFILSLLLKVIQSIKCEDSLLLLQRFCGIQRFADSICSGVSN 1680
            +NP+IGLNTLYKELKTSGGF+LSLLLKVI S+KCEDSLLLLQR CGIQRFADSICSG+SN
Sbjct: 1621 MNPSIGLNTLYKELKTSGGFVLSLLLKVIHSVKCEDSLLLLQRLCGIQRFADSICSGMSN 1680

Query: 1681 ENANDSCGQGILHILTSLRVELPQFDIQFLSRGSDPVLAHDPFASVMWVLFCLPCPFLSC 1740
            ENA+DSCG+GILHILTSLR ELP FD QFLSRGSDPV+AHDPFAS+MWVLFCLP PFLSC
Sbjct: 1681 ENASDSCGRGILHILTSLRSELPHFDSQFLSRGSDPVIAHDPFASLMWVLFCLPFPFLSC 1740

Query: 1741 KESLISLVHIFYLVSVAQGIIASCMKYQSKIDGMGFSDCLIADICKIMGESEYARRYFVS 1800
            +ESL+SLVHIFYLVSV Q II    K Q K+DG+G SDCLI DICKIMGESEYAR+YFVS
Sbjct: 1741 RESLLSLVHIFYLVSVTQAIITYFTKSQCKVDGLGSSDCLITDICKIMGESEYARQYFVS 1800

Query: 1801 DYTEPSCNVKDMIRSLTFTYLRRCALLLKLLNSSARAPIFDGETALERYLAVDDMIDNIA 1860
            +YTEPSCN+KD +RSLTF +LRRCALLL+LL+SSAR PIFDGETALE YL  ++MIDNI 
Sbjct: 1801 NYTEPSCNIKDTVRSLTFPFLRRCALLLQLLSSSARVPIFDGETALETYLVGNNMIDNIT 1860

Query: 1861 LELNEIGKLEKIFKIPPLDVVLKDEASRSLVWKWFCHFNREFEFQRSKNLKHCTPAVPFQ 1920
            +ELNE+ KL+K+F IPPLD+VLKD  SR LV KWFCHFN+EFEFQRSK +KHCTPAVPFQ
Sbjct: 1861 VELNEVEKLQKMFDIPPLDIVLKDGTSRLLVSKWFCHFNKEFEFQRSKIIKHCTPAVPFQ 1920

Query: 1921 LIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANIC 1980
            LIRLPHVYHDLLQRYIKKRC DCKHVIDDPALCL+CG+LCSPSWKSCCRESGCQAHANIC
Sbjct: 1921 LIRLPHVYHDLLQRYIKKRCTDCKHVIDDPALCLICGKLCSPSWKSCCRESGCQAHANIC 1980

Query: 1981 AAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYM 2040
            AAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYM
Sbjct: 1981 AAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYM 2036

BLAST of MS019737 vs. NCBI nr
Match: XP_022983922.1 (E3 ubiquitin-protein ligase PRT6 [Cucurbita maxima])

HSP 1 Score: 3531.5 bits (9156), Expect = 0.0e+00
Identity = 1781/2041 (87.26%), Postives = 1881/2041 (92.16%), Query Frame = 0

Query: 1    MDDMDIGSPSESAPLKPRDRIVRRLALLGVPEELLDQLQPGLVSFVKDNKILIPQLVSAI 60
            MDDMDIGSPSESAPLKPRDRI+RRLA+ GVP+ELLDQL  GLVSFVKDNK LIPQLVSAI
Sbjct: 1    MDDMDIGSPSESAPLKPRDRILRRLAVFGVPDELLDQLFCGLVSFVKDNKFLIPQLVSAI 60

Query: 61   LPTDVEVAEVIRDAKAGSKKSMAGPTMKNQFRESMVWLQWLMFEGEPTFALKNLSKMSFG 120
            LPTDVEV E IRDAKAGSKKS+ GPTMK QFRESM+WLQWLMFEGEP +ALKNLSKMS G
Sbjct: 61   LPTDVEVVEFIRDAKAGSKKSLTGPTMKTQFRESMMWLQWLMFEGEPAYALKNLSKMSIG 120

Query: 121  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVT 180
            QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNH+DHDYSIIYTGGGCCDCGD T
Sbjct: 121  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHQDHDYSIIYTGGGCCDCGDAT 180

Query: 181  AWKREGFCSKHKGAERIQPLPEEFVESVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 240
            AWKREGFCSKHKGAE+IQPLPEEFV+SVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV
Sbjct: 181  AWKREGFCSKHKGAEQIQPLPEEFVKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 240

Query: 241  MEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISLAGFLDILVRLERLLTDIVVKK 300
             EHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVIS AG LDILVRLERLLTD VVKK
Sbjct: 241  TEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGLLDILVRLERLLTDSVVKK 300

Query: 301  VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHALKKYPLLPTFSVQIFTVPT 360
            VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIE+ SDHALKKYPLLPTFSVQIFTVPT
Sbjct: 301  VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEDSSDHALKKYPLLPTFSVQIFTVPT 360

Query: 361  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAV 420
            LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVED+RFVMSHAV
Sbjct: 361  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDVRFVMSHAV 420

Query: 421  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHTL 480
            VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIH+L
Sbjct: 421  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL 480

Query: 481  LVNEAFSAASSSSSSEETADAMSFHAFKQNPDDMDGIRHAKVGRLSQESAACNVFGRSST 540
            LV EAFS A + SS E+TADAMSF  +KQN DD+D +RHAKVGRLSQE+AACNV G+ S 
Sbjct: 481  LVKEAFSPA-NCSSREDTADAMSFQTYKQNVDDIDNVRHAKVGRLSQETAACNVLGKGSR 540

Query: 541  VTSASRVDEVCSDTISSTIMWLTYECLKVIDSWLGTENVLGGFPNMFAENASMASSCKVY 600
             TSASRVDEVC D IS TIMWLTYECLK+IDSWL TE+  G  PNM  ++ S++ SCK Y
Sbjct: 541  STSASRVDEVCLDKISPTIMWLTYECLKIIDSWLATESTYGSIPNMLDDSISLSPSCKSY 600

Query: 601  SLRKPSALESRKISSKVEKGKFIFEKVAGKSKDHNRQYSSRIYSGLQMSIDNEHGVSLGE 660
            SLRK SA       SK+EKGKFIFEK+A +SK H RQYSSR+YSGLQMSIDNEHG+S GE
Sbjct: 601  SLRKTSA------PSKMEKGKFIFEKLAKRSKYHIRQYSSRMYSGLQMSIDNEHGISCGE 660

Query: 661  DNQLMDVTNDTVTVEDYSMETDALHFLSFSAWPNIVYDVSSQDISIHIPLHRLLSLLLQK 720
            D+QLMDVTNDTVT EDY+ME DALHFLS SAWPNIVYDVSSQDISIH+PLHRLLSLLLQK
Sbjct: 661  DSQLMDVTNDTVTDEDYAMEVDALHFLSSSAWPNIVYDVSSQDISIHVPLHRLLSLLLQK 720

Query: 721  ALRSSFSESVVPSATGASSSNLSFEYVDFFKSVLTDYHPYGFSAFVMEHPLRIKVFCAEV 780
            AL S FSESVVPSATGASSSNLS EYVDFFKSVLTD HPYGFS+FVMEHPLRIKVFCAEV
Sbjct: 721  ALGSCFSESVVPSATGASSSNLSSEYVDFFKSVLTDCHPYGFSSFVMEHPLRIKVFCAEV 780

Query: 781  NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPELYISRILERFRLLD 840
            NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPP+LY+SRI+ERFRL D
Sbjct: 781  NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVSRIIERFRLSD 840

Query: 841  YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQL 900
            Y+SLNVERPSEYEP LVQEMLTLIIQ+VNERRFCGLTVAESLKRELIYKLAIGDATHSQL
Sbjct: 841  YISLNVERPSEYEPTLVQEMLTLIIQIVNERRFCGLTVAESLKRELIYKLAIGDATHSQL 900

Query: 901  VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLRWKYWKELDLYHPRWSLRDLQIA 960
            VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSL WKYWKELDLYHPRWSLRDLQ+A
Sbjct: 901  VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPRWSLRDLQVA 960

Query: 961  EERYLRSCGASALTSQLPKWTKIYPPFRGLARIATCKAVLQIIRAVLFYSVFTDKSTKSR 1020
            EERYLRSC  SALTSQLPKWTK YPPF+GLARIATCK VLQ +RAVLFYSVFT+KSTKSR
Sbjct: 961  EERYLRSCSVSALTSQLPKWTKTYPPFKGLARIATCKTVLQFVRAVLFYSVFTEKSTKSR 1020

Query: 1021 APDSVLLTALHLLALALDICFQRKESSDQSFCSEDSIPLLLFADEEVDEGLTYGLGRQSL 1080
            APDSVLL+ALHLLALALDICFQ+KESSDQSF   DSIPLLLFA EE+DEGL YG GRQSL
Sbjct: 1021 APDSVLLSALHLLALALDICFQQKESSDQSFYGPDSIPLLLFAAEEIDEGLAYGFGRQSL 1080

Query: 1081 LSLLILLMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDSRCMGKIRQHAPEILGYL 1140
            LSLLILLMKMHKKEGREN LEAGSCNLSSLVESLLKKFSE+DS CMGK++Q APEILGYL
Sbjct: 1081 LSLLILLMKMHKKEGRENFLEAGSCNLSSLVESLLKKFSEVDSHCMGKVQQLAPEILGYL 1140

Query: 1141 SQSLPSSDPNSLSETSDSDKLKAKARERQAAILEKMRAEQYKFLASVDSSV-EDDAEFGQ 1200
            S+SLPSS+ N  S +SDS+K KAKARERQAAILEKMRAEQ KFLASVD+SV +DD EFGQ
Sbjct: 1141 SESLPSSNTNRPSRSSDSEKRKAKARERQAAILEKMRAEQSKFLASVDASVDDDDPEFGQ 1200

Query: 1201 ESEKPSVSDSAEQSETVCSLCHDSSSSVPISYLILLQKSKLVSLIDRGTVSWDQPYWRDE 1260
            ESEKP VS SAEQSETVCSLCHDSSSSVPIS+L LLQKSKLVSLIDRG VSWDQPYWRDE
Sbjct: 1201 ESEKPDVSVSAEQSETVCSLCHDSSSSVPISFLTLLQKSKLVSLIDRGVVSWDQPYWRDE 1260

Query: 1261 HASSIPRRNLDQSGLSTSSGGSGGISSPQLAELIQNAVKEYASHGLPGEVGAFLDFVKSH 1320
            HAS++ +RNLDQSGLSTSS  SGGISS QL ELIQNAVKEYA+HGLPGEVGAFLDFVKSH
Sbjct: 1261 HASTMSKRNLDQSGLSTSSASSGGISSLQLTELIQNAVKEYANHGLPGEVGAFLDFVKSH 1320

Query: 1321 FPPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHENMHSKLNEDENFSKVASSL 1380
            FPPLRN QVPGTSN KGEKIIFSFDTLEEDIYLSV+KEMH+++HSK  EDENFSK     
Sbjct: 1321 FPPLRNSQVPGTSNDKGEKIIFSFDTLEEDIYLSVQKEMHDSLHSKFYEDENFSKAVRG- 1380

Query: 1381 VTEDSKSVLHVKYIAALSRELAENHSTSESAHNVSMPVESLQPTTFNEIDPVDCDGIYLS 1440
              EDS SVLHVKYIAALSRELAENH+TS SAHNV   VE LQPT FNEI P DCDGIYLS
Sbjct: 1381 --EDSMSVLHVKYIAALSRELAENHATSVSAHNVDTLVEHLQPTAFNEIGPTDCDGIYLS 1440

Query: 1441 SCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAFPRE 1500
            SCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLAN TLPA+PRE
Sbjct: 1441 SCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANCTLPAYPRE 1500

Query: 1501 FQKFGNPSMSSIGSLSHVSGQSNKSTEEVNPLYVQKAVAFLQSTAKAVGKNKVLKDISVH 1560
             Q   NP+ SS+GSLS VSG  NKSTE  NPLY+Q+AVA LQS AKAVG+NKVLKDISVH
Sbjct: 1501 SQNIWNPATSSVGSLSRVSGHLNKSTERGNPLYIQQAVALLQSAAKAVGRNKVLKDISVH 1560

Query: 1561 RHRKVSRNLEAVSLVLSKLYFSGKQDKSISCTRVNPSILMWDTLKYSLVSMEIAARSKTD 1620
             HRKVS +LE VSLVLSK+YFS KQDK IS +R+NPSILMWD LKYSL+SMEIAARSKTD
Sbjct: 1561 GHRKVSHDLEVVSLVLSKMYFSEKQDKLISSSRINPSILMWDMLKYSLISMEIAARSKTD 1620

Query: 1621 VNPNIGLNTLYKELKTSGGFILSLLLKVIQSIKCEDSLLLLQRFCGIQRFADSICSGVSN 1680
            +NPNIGLNTLYKELKTSGGF+LSLLLKVIQ +KCEDSLLLLQRFCGIQRFADSICSG+SN
Sbjct: 1621 MNPNIGLNTLYKELKTSGGFVLSLLLKVIQRVKCEDSLLLLQRFCGIQRFADSICSGMSN 1680

Query: 1681 ENANDSCGQGILHILTSLRVELPQFDIQFLSRGSDPVLAHDPFASVMWVLFCLPCPFLSC 1740
            ENA+DSCG+GILHILTSLRVELPQFDIQFLSRGSDPVLAHDPFAS+MWVLFCLP PFLSC
Sbjct: 1681 ENASDSCGRGILHILTSLRVELPQFDIQFLSRGSDPVLAHDPFASLMWVLFCLPFPFLSC 1740

Query: 1741 KESLISLVHIFYLVSVAQGIIASCMKYQSKIDGMGFSDCLIADICKIMGESEYARRYFVS 1800
            +ESL+SLVHIFYLVSVAQ II+S +K Q K+D +GFSD L+ADICKIM ESE+AR+YFVS
Sbjct: 1741 RESLLSLVHIFYLVSVAQAIISSFIKSQYKVDELGFSDSLVADICKIMEESEHARQYFVS 1800

Query: 1801 DYTEPSCNVKDMIRSLTFTYLRRCALLLKLLNSSARAPIFDGETALERYLAVDDMIDNIA 1860
            +YTEPSCNVKDMIRS TF YLRRCALLLKLLNS A  PIFDGETALE YLA ++MIDN  
Sbjct: 1801 NYTEPSCNVKDMIRSWTFPYLRRCALLLKLLNSCALVPIFDGETALETYLAGNNMIDNNT 1860

Query: 1861 LELNEIGKLEKIFKIPPLDVVLKDEASRSLVWKWFCHFNREFEFQRSKNLKHCTPAVPFQ 1920
            LELNE  KLEKIF+I PLD+V KD  SR+LV KWFCHFN+EFEFQRSKN+KHCTPAVPFQ
Sbjct: 1861 LELNENEKLEKIFEILPLDIVFKDGTSRALVSKWFCHFNKEFEFQRSKNIKHCTPAVPFQ 1920

Query: 1921 LIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANIC 1980
            LIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANIC
Sbjct: 1921 LIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANIC 1980

Query: 1981 AAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYM 2040
            AAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYM
Sbjct: 1981 AAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYM 2031

BLAST of MS019737 vs. ExPASy Swiss-Prot
Match: F4KCC2 (E3 ubiquitin-protein ligase PRT6 OS=Arabidopsis thaliana OX=3702 GN=PRT6 PE=1 SV=1)

HSP 1 Score: 1794.6 bits (4647), Expect = 0.0e+00
Identity = 1009/2054 (49.12%), Postives = 1343/2054 (65.38%), Query Frame = 0

Query: 19   DRIVRRLALLGVPEELLDQLQPGLVSFVKDNKILIPQLVSAILPTDVEVAEVIRDAKAGS 78
            D ++ RLA +GVP++   + + GLV FV+ N   I +LVSA+LPTD +V   +++A+   
Sbjct: 17   DLVIERLASVGVPKKY--RSKRGLVEFVRANPAKISELVSALLPTDDDVKLGLKEARERP 76

Query: 79   KKSMAGPTMKNQFRESMVWLQWLMFEGEPTFALKNLSKMSFGQRGVCGAVWGHNDIAYRC 138
            +KS   PTMK +FRESM  LQWLMF+ EP  +L+NL+K++  QRGVCG+VWG NDIAYRC
Sbjct: 77   RKSAVSPTMKKRFRESMNMLQWLMFQDEPDVSLRNLAKLNLDQRGVCGSVWGQNDIAYRC 136

Query: 139  RTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVTAWKREGFCSKHKGAERIQ 198
            RTCE+DPTCAICVPCFQ+G+H  HDYSIIYTGGGCCDCGD TAWK +GFCS HKG+E+I+
Sbjct: 137  RTCENDPTCAICVPCFQNGDHNSHDYSIIYTGGGCCDCGDETAWKPDGFCSNHKGSEQIR 196

Query: 199  PLPEEFVESVGPILDALFTSWKNKLLSAEDISVEDPKLSDRVMEHKKVANELTFAVVEML 258
            PL E    SVGPILDALFT W NKLLSAE    +  + +D ++  +K++NELTF VVEML
Sbjct: 197  PLSENLANSVGPILDALFTCWNNKLLSAESSGQKGARSNDTLVILQKMSNELTFIVVEML 256

Query: 259  LDFCKYSESLLSFVSKRVISLAGFLDILVRLERLLTDIVVKKVHELLLKLLGEPIFKYEF 318
            L+F   SESLLSFVS+R+IS +G L IL++ ER L   V+KK+H+L LKL+G+P+FK EF
Sbjct: 257  LEFSMSSESLLSFVSRRIISSSGLLSILLKAERFLDQDVMKKLHDLFLKLIGDPVFKCEF 316

Query: 319  AKVFLNYYPTVISEAIEEGSDHALKKYPLLPTFSVQIFTVPTLTPRLVEEMNLLSILLGC 378
            AK F++YYP VISE +++G+D+A KKYPLL TFSVQI TVPTLTP LV+EMNLL++LLGC
Sbjct: 317  AKAFVSYYPVVISEVVKQGTDNAFKKYPLLSTFSVQILTVPTLTPFLVKEMNLLAMLLGC 376

Query: 379  LEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAVVPRYVIYEQQDILRTWLR 438
            L DIF+SC  EDG LQ  K   L ET+ RV+ D++FVMSHA+V +Y  +E +++ R+WL 
Sbjct: 377  LSDIFVSCSGEDGLLQATKLERLCETSERVIGDLKFVMSHAIVSKYATHEHRELSRSWLT 436

Query: 439  LLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHTLLVNEAFSAASSSSSSEET 498
            LLTF QGM+P KRETG+ I+EEN+ +HL F L HS+A IH+LLVN  +SAAS      + 
Sbjct: 437  LLTFAQGMNPLKRETGIPIDEENDYMHLFFVLGHSIAVIHSLLVNGTYSAASDEEIENDR 496

Query: 499  ADAMSFHAFKQNPDDMDGIRHAKVGRLSQESAACNVFGRSSTVTS--ASRVDEV--CSDT 558
                 F     +  D DG R+AKVGRLS E + C     SS+  S  AS V ++      
Sbjct: 497  NAKEEF-----DKCDGDGERYAKVGRLSHEDSVCTAIVSSSSFDSSMASEVHKIDPFHAL 556

Query: 559  ISSTIMWLTYECLKVIDSWLGTENVLGGFPNMFAENASMASSCKVYSLRKPSALES---- 618
            + S+ ++L  ECLKV+++ LG +  +  F             CK+ S    +  ES    
Sbjct: 557  LPSSAIYLIRECLKVLETCLGNDEGISKF------------LCKLSSSSGRNIPESKMSW 616

Query: 619  -RKISSKVEKGKFIFEKVAGKSKDHNRQYSSRIYSGLQ--MSIDNEHG-VSLGEDNQLMD 678
             R+    VE G  +   +A  S+D +   S  +   +Q  +S+DN  G   + + +   D
Sbjct: 617  PRRDLLNVETGGSVSSNLASSSRDPSTGLSP-LCGDIQTNLSLDNVCGPYGVVQTDVTAD 676

Query: 679  VTNDTVTVEDYSMETDALHFLSFSAWPNIVYDVSSQDISIHIPLHRLLSLLLQKALRSSF 738
                +    D +     L  L    WP+I YDVSSQ IS+H+PLHRLLSLL+QKALR  +
Sbjct: 677  SKRVSCNSADLTKNASGLRILGLCDWPDIHYDVSSQAISVHLPLHRLLSLLIQKALRICY 736

Query: 739  SESVVPSATGASSSNLSFEYVDFFKSVLTDYHPYGFSAFVMEHPLRIKVFCAEVNAGMWR 798
             ES   S  G S S+    + DFF SV+ D+HP GFSA VMEH L+I+VFCA+V AGMW+
Sbjct: 737  GESA--SYNGVSISH-EIPHADFFSSVIGDFHPCGFSALVMEHVLQIRVFCAQVIAGMWK 796

Query: 799  RNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPELYISRILERFRLLDYLSLNV 858
            +NGD+AL+SCE YRS+RWSEQ LELDLFLLQCCAA+AP + Y+ ++L RF L  YLSLN 
Sbjct: 797  KNGDSALVSCEWYRSVRWSEQGLELDLFLLQCCAALAPADSYVDKLLSRFGLSSYLSLNP 856

Query: 859  ERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQLVKALPR 918
            +  +EYEP+LVQEML L+IQ++ ERRFCGL+ AESL+RE+I+KLA GD THSQLVK+LPR
Sbjct: 857  DITNEYEPVLVQEMLGLLIQILQERRFCGLSTAESLRREIIFKLATGDFTHSQLVKSLPR 916

Query: 919  DLSKCHQLQEILDTIAVYSNPSGFNQGMYSLRWKYWKELDLYHPRWSLRDLQIAEERYLR 978
            DLSK  +LQE+LD ++VY NPSG NQG YSL+   WKELDLYHPRW  RDLQ AEER+ R
Sbjct: 917  DLSKSDELQEVLDDVSVYCNPSGMNQGKYSLQSSCWKELDLYHPRWQSRDLQSAEERFSR 976

Query: 979  SCGASALTSQLPKWTKIYPPFRGLARIATCKAVLQIIRAVLFYSVFTDKSTKSRAPDSVL 1038
             CG SALT+QLP+W  IYPP +GLARI TCKA  QII + L+Y++ +  S KSRAPD VL
Sbjct: 977  YCGVSALTTQLPRWRMIYPPLKGLARIGTCKATFQIISSALYYALQSGTSVKSRAPDGVL 1036

Query: 1039 LTALHLLALALDICFQRKESSDQSFCSEDSIPLLLFADEEVDEGLTYGLGRQSLLSLLIL 1098
            +TAL LL+L+LDIC Q+++S+ Q  C E+SIP+L  A  E+  G+  G  ++SLLSLL+ 
Sbjct: 1037 ITALQLLSLSLDICTQQRQSNSQDCCLENSIPILELAGLEI-IGIAQGTEKESLLSLLVS 1096

Query: 1099 LMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDSRCMGKIRQHAPEILGYLSQSLPS 1158
            LMK    +GR    E GSCN+SS + +LLKKFS IDS CM  ++  APE++G       S
Sbjct: 1097 LMKTRMGDGRHQFPEPGSCNISSWIGNLLKKFSAIDSVCMNLLQSLAPEVVG------QS 1156

Query: 1159 SDPNSLSETSDSDKLKAKARERQAAILEKMRAEQYKFLASVDSSVEDD---AEFGQESEK 1218
                 +S ++  +K KAKA+ERQAAI+ KM+AEQ KFL+++ SS++DD   +EF + S+ 
Sbjct: 1157 GFDKVMSGSTSDEKRKAKAKERQAAIMAKMKAEQSKFLSTLSSSMDDDDPRSEF-ETSDS 1216

Query: 1219 PSVSDSAEQSETVCSLCHDSSSSVPISYLILLQKSKLVSLIDRGTVSWDQPYWRDEHASS 1278
                DS      VCSLCHD  S  P+S+LI LQKSKL+S +DRG  SWDQ    ++  S 
Sbjct: 1217 VMEHDSEIAVREVCSLCHDPDSKDPVSFLIFLQKSKLLSFVDRGPPSWDQCPQSEKKISV 1276

Query: 1279 IPRRNLDQSGLSTSSGGSGGISSPQLAELIQNAVKEYASHGLPGEVGAFLDFVKSHFPPL 1338
                +L +     +S  S  ISSP + +L  + + E A+          ++ +K+    +
Sbjct: 1277 DGAPDLLR---MNASSDSLRISSPLMLQLSDDTISESAN---------MIESIKARL--I 1336

Query: 1339 RNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHENMHSKLNEDENFSKVASSLVTED 1398
             N Q    S+    K   + ++LE  +Y +VR ++   ++  L   ++    A +   ++
Sbjct: 1337 GNGQTEKRSSDGRGKDESNMESLEIAMYQTVRNKIENMINQSLTRVDHQPHEAENCSEKN 1396

Query: 1399 S---KSVLHVKYIAALSRELAENHSTSESAHNVSMPVESLQPTTFNEIDPVDCDGIYLSS 1458
            S    S L  ++    SR+ +          +                 P+DCDG+YLSS
Sbjct: 1397 SVGGPSTLQGRFPDIRSRQTSRRPDAGSDGFH-----------------PIDCDGVYLSS 1456

Query: 1459 CGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAFPREF 1518
            CGHAVHQ CL+RYL SLKER  RR VFEG HIVD ++ EFLCPVCRRLANS LP  P + 
Sbjct: 1457 CGHAVHQSCLERYLKSLKERSGRRTVFEGAHIVDLKKKEFLCPVCRRLANSVLPECPGD- 1516

Query: 1519 QKFGNPSMSSIGSLSHVSGQSNKSTEEVNP-LYVQKAVAFLQSTAKAVGKNKVLKDISVH 1578
                   + S+  L        +  + + P L++ +A+  L+S A+ +      K ++  
Sbjct: 1517 -------LCSVSKLQDSPRTKLRRKDALQPSLWLSEALCLLRSAAEVIEDGDRGKTVTPQ 1576

Query: 1579 RHRKVSRNLEAVSLVLSKLYFSGKQDKSISCTRVNP-SILMWDTLKYSLVSMEIAAR-SK 1638
                  ++L++VS +L   YF   +DK++    + P SI+MWDTLKYSL+SMEI  R +K
Sbjct: 1577 GDGPRRKDLKSVSKMLWDFYFPKPEDKTLKRLWLPPQSIVMWDTLKYSLISMEIGTRFAK 1636

Query: 1639 TDVNPNIGLNTLYKELKTSGGFILSLLLKVIQSIKCEDSLLLLQRFCGIQRFADSICSGV 1698
              + P   +++LY+ELKTS G ILS+LL+V+QS + ++++ + QRF G++  A+SIC GV
Sbjct: 1637 NSMLPVYCIDSLYEELKTSKGTILSVLLRVVQSSRTKNTIHVRQRFVGMKHLAESICYGV 1696

Query: 1699 SNENANDSCGQGILHILTSLRVELPQFDIQFLSRGSDPVLAHDPFASVMWVLFCLPCPFL 1758
            S+ +++   G             L   D+ + +R SDPVLAHDPF+S+MW LFCLP PFL
Sbjct: 1697 SSSSSSSIFGS------EGTTGSLKNIDLLW-NRASDPVLAHDPFSSLMWALFCLPFPFL 1756

Query: 1759 SCKESLISLVHIFYLVSVAQGIIASCMKYQSKIDGMGFSDCLIADICKIMGESEYARRYF 1818
            +C+ESL+SLVHIF+ VS+ Q +IA C    S++  + F + L+ DI   + ES     YF
Sbjct: 1757 TCEESLLSLVHIFHSVSLVQTVIAYCACRPSELSELNFGENLLNDISNALRESG-GWEYF 1816

Query: 1819 VSDYTEPSCNVKDMIRSLTFTYLRRCALLLKLLNSSAR-----APIFDGETALERYLAVD 1878
             S+  + SC++KD IR  +  +LRRCALL KLL S+ R     + +FD        L  D
Sbjct: 1817 RSNNMDLSCDIKDTIRKYSLPFLRRCALLWKLLKSTPRKLHEESDMFD--------LPSD 1876

Query: 1879 DMIDNIAL------ELNEIGKLEKIFKIPPLDVVLKDEASRSLVWKWFCHFNREFEFQRS 1938
               DN+        ELN + +LEK+F IPP+D++L DE  RS    W  HF RE+   R 
Sbjct: 1877 PTTDNMDFIYSPQSELNHVQELEKMFNIPPIDIILNDELLRSSTQIWLQHFQREYRVNRV 1936

Query: 1939 KNLKHCTPAVPFQLIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSC 1998
            K     TP VPFQL++LP++Y DLLQR IKKRC +C  VI++P LCLLCG LCSP W  C
Sbjct: 1937 KRSLCITPVVPFQLMKLPNLYQDLLQRCIKKRCVNCTKVIEEPVLCLLCGSLCSPIWSPC 1984

Query: 1999 CRESGCQAHANICAAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPL 2041
            CRESGC  HA  C AGTGVFLLIRRTTILLQR ARQ+PWPSPYLD FGEEDI+M RGK L
Sbjct: 1997 CRESGCPNHAITCGAGTGVFLLIRRTTILLQRFARQSPWPSPYLDTFGEEDIDMIRGKRL 1984

BLAST of MS019737 vs. ExPASy Swiss-Prot
Match: Q6ZT12 (E3 ubiquitin-protein ligase UBR3 OS=Homo sapiens OX=9606 GN=UBR3 PE=2 SV=2)

HSP 1 Score: 219.9 bits (559), Expect = 2.6e-55
Identity = 433/2039 (21.24%), Postives = 754/2039 (36.98%), Query Frame = 0

Query: 118  SFGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYT-GGGCCDC 177
            ++    +CG VW  N +AYRCRTC   P  ++C  CF  G+H  HD+++  +  GG CDC
Sbjct: 113  AYDPAALCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGDHTGHDFNMFRSQAGGACDC 172

Query: 178  GDVTAWKREGFCSKH--KGAERIQPLPEEFVESVGPILDALFTSWKNKLLSAEDISVEDP 237
            GD    +  GFC +H  K +  I  +P++ +     +L          L+        +P
Sbjct: 173  GDSNVMRESGFCKRHQIKSSSNIPCVPKDLLMMSEFVLPRFIFC----LIQYLREGYNEP 232

Query: 238  KLSDRVMEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISLAGFLDILVRLERLLT 297
                   +      +L    +  L D  K   ++ S +++ + +   + D+   L     
Sbjct: 233  AADGPSEKDLNKVLQLLEPQISFLEDLTKMGGAMRSVLTQVLTNQQNYKDLTSGLGE--- 292

Query: 298  DIVVKKVHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHALKKYPLLPTFSVQ 357
            +  VKK HE  L  L      Y   K+            ++E S           + +VQ
Sbjct: 293  NACVKKSHEKYLIALKSSGLTYPEDKLVY---------GVQEPSAGT-------SSLAVQ 352

Query: 358  IFTVPTLTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRF 417
             F   T T   V+  +         ED       +DG   + K   +  ++    + I  
Sbjct: 353  GFIGATGTLGQVDSSD---------ED------DQDGSQGLGKRKRVKLSSGTKDQSIMD 412

Query: 418  VMSHAVVPRYVIYEQQDILRTWLRLLTF--VQGMDPQKRETGLHIEEENENVHLPFGLDH 477
            V+ H               +++L  L F  ++   PQK  T L        +++    ++
Sbjct: 413  VLKH---------------KSFLEELLFWTIKYEFPQKMVTFL--------LNMLPDQEY 472

Query: 478  SVANIHTLLVNEAFSAASSSSSSEETADAMSFHAFKQNPDDMDGIRHAKVGRLSQESAAC 537
             VA   T + + AF   +   S E  +D MS           + I H  V   S E    
Sbjct: 473  KVAFTKTFVQHYAFIMKTLKKSHE--SDTMS-----------NRIVHISVQLFSNEEL-- 532

Query: 538  NVFGRSSTVTSASRVDEVCS--DTISSTIMWLTYECLKVIDSWLGTENVLGGFPN----M 597
                       A +V E C   D + + ++++   CL +       EN L    N    +
Sbjct: 533  -----------ARQVTEECQLLDIMVTVLLYMMESCL-IKSELQDEENSLHVVVNCGEAL 592

Query: 598  FAENASMASSCKVYSLRKPSALESRKISSK---VEKGKFI--FEKVAGKSKDHNR--QYS 657
               N          ++    ++  R +      V    F+  F+ +    ++ N   ++ 
Sbjct: 593  LKNNTYWPLVSDFINILSHQSVAKRFLEDHGLLVTWMNFVSFFQGMNLNKRELNEHVEFE 652

Query: 658  SRIY---SGLQMSIDNEHGVSLGEDNQLMDVTNDTVTVEDYSMET-----DALHFLSFSA 717
            S+ Y      ++    +    L    ++ +    T  V  Y +E      DA++F+   A
Sbjct: 653  SQTYYAAFAAELEACAQPMWGLLSHCKVRETQEYTRNVVRYCLEALQDWFDAINFVDEPA 712

Query: 718  WPNIVYDVSSQDISIHIPLHRLLSLLLQKALRSSFSESVVPSATGASSSNLSFEYVDFFK 777
             PN         ++ H+PLHR  ++ L KA++    +                       
Sbjct: 713  -PN--------QVTFHLPLHRYYAMFLSKAVKCQELD---------------------LD 772

Query: 778  SVLTDYHPYGFSAFVMEHPLRIKVFCAEVNAGMWRRNGDAALLSCELYRSIRWSEQCLEL 837
            SVL D         +M HPL+I+   AE+++ MW RNG         Y    +    ++ 
Sbjct: 773  SVLPDQE---MLMKLMIHPLQIQASLAEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDP 832

Query: 838  DLFLLQCCAAMAPPELYISRILERFRLLDYLSL-----NVERPSEYEPILVQEMLTLIIQ 897
            D++LLQ CA+   P+ +IS + ERF+++D L++     N    +E+E  +++  LT ++ 
Sbjct: 833  DIYLLQVCASRLDPDYFISSVFERFKVVDLLTMASQHQNTVLDAEHERSMLEGALTFLVI 892

Query: 898  VVNERRFCGLTVAESLKRELIYKLAIGDATHSQLVKALPRD-------LSKCHQLQEILD 957
            +++ R   G++  E L+ E++ +L + D THS L+  +P +       +   +  + +L 
Sbjct: 893  LLSLRLHLGMSDDEILRAEMVAQLCMNDRTHSSLLDLIPENPNPKSGIIPGSYSFESVLS 952

Query: 958  TIAVYSNP-----SGFNQGMYSLRWKYW-KELD---LYHPRWSLRDLQIAEERY---LRS 1017
             +A +  P         QGMY+ + + W +E D   +       RD+Q A +RY   L+ 
Sbjct: 953  AVADFKAPVFEPGGSMQQGMYTPKAEVWDQEFDPVMVILRTVYRRDVQSAMDRYTAFLKQ 1012

Query: 1018 CGASALTSQLP--KWTKIYPPFRGLARIATCKAVLQIIRAVLFYSVFTDKSTKSRAPDSV 1077
             G        P  K T ++P ++GL R+  CK  L I+   L Y +  D    S   + V
Sbjct: 1013 SGKFPGNPWPPYKKRTSLHPSYKGLMRLLHCK-TLHIVLFTLLYKILMDHQNLS---EHV 1072

Query: 1078 LLTALHLLALALDICFQRKESSDQSFCSEDSIPLLLFADEEVDEGLTYGLGRQSLLSLLI 1137
            L   L+L+ L L+   + +   + S    +      F    +   +     R  +  + +
Sbjct: 1073 LCMVLYLIELGLENSAEEESDEEASVGGPERCHDSWFPGSNLVSNM-----RHFINYVRV 1132

Query: 1138 LLMKMHKKEGRENLLEAGSCNLSSLVES-LLKKFSEIDSRCMGKIRQHAPEILGYLSQSL 1197
             + +   +  R++     S NL SL  S   + FS +  R     R+   EI+     + 
Sbjct: 1133 RVPETAPEVKRDSPASTSSDNLGSLQNSGTAQVFSLVAER-----RKKFQEII-----NR 1192

Query: 1198 PSSDPNSLSETSDSDKLKA--KARERQAAILEKMRAEQYKFLASVDSSVEDDAEFGQESE 1257
             SS+ N +     S K  A   A +   AILE   +     + S+   +       Q S 
Sbjct: 1193 SSSEANQVVRPKTSSKWSAPGSAPQLTTAILEIKES-----ILSLLIKLHHKLSGKQNSY 1252

Query: 1258 KPSVSDSAE---QSETVCSLCHDSSSSVPISYLILLQKSKLVSLIDRGTVSWDQPYWRDE 1317
             P   D  E   Q E       D  ++V    L    +S++   I         P    +
Sbjct: 1253 YPPWLDDIEILIQPEIPKYSHGDGITAVERILLKAASQSRMNKRIIEEICRKVTPPVPPK 1312

Query: 1318 HASSIPRRNLDQSGLSTSSGGSGGISSPQLAELIQNAVKEYASHGLPGEVGAFLDFVKSH 1377
              ++  ++ LD+                +  E  Q  + E+AS              KS 
Sbjct: 1313 KVTAAEKKTLDKEE-----------RRQKARERQQKLLAEFASR------------QKSF 1372

Query: 1378 FPPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHENMHSKLNEDENFSKVA--- 1437
                 ++  P         I     T E  +  +V   +        +ED     V    
Sbjct: 1373 METAMDVDSPEND------IPMEITTAEPQVSEAVYDCVICGQSGPSSEDRPTGLVVLLQ 1432

Query: 1438 SSLVTEDSKSVLHVKYIAALSRELAENHSTSESAHNVSMPVESLQPTTFNEIDPVDC--- 1497
            +S V    +  +  K +     E      T  + H+V +   SL    F +   +     
Sbjct: 1433 ASSVLGQCRDNVEPKKLPISEEEQIYPWDTCAAVHDVRL---SLLQRYFKDSSCLLAVSI 1492

Query: 1498 ---DGIYLSSCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLAN 1557
                G+Y+ +CGH +H  C   Y+ SL+       V +G  +   ++GEF CP+CR+ AN
Sbjct: 1493 GWEGGVYVQTCGHTLHIDCHKSYMESLR----NDQVLQGFSV---DKGEFTCPLCRQFAN 1552

Query: 1558 STLPAFPR---EFQKFGNPSMSSIGSLSHVSGQSNKSTEEVNPLYVQKAVAFLQSTAKAV 1617
            S LP +P    E   +  PS  SI  L        K  EE+      +  AF   T  + 
Sbjct: 1553 SVLPCYPGSNVENNPWQRPSNKSIQDLI-------KEVEELQ----GRPGAFPSETNLSK 1612

Query: 1618 GKNKVLKDISVHRHRKVSRNLEAVSLVLSKLYFSGKQDKSISCTRVNPSILMWDTLKYSL 1677
                V+KDI     +K                                            
Sbjct: 1613 EMESVMKDIKNTTQKK-------------------------------------------- 1672

Query: 1678 VSMEIAARSKTDVNPNIGLNTLYKELKTSGGFILSLLLKVIQSIKCEDSLLLLQRFCGIQ 1737
                    SKT  +P+     +Y   +T      +L L++I                   
Sbjct: 1673 ----YRDYSKTPGSPDNDFLFMYSVART------NLELELIHR----------------- 1732

Query: 1738 RFADSICSGVSNENANDSCGQGILHILTSLRVELPQFDIQF------------------- 1797
                ++CSG ++     SC   + H+L +L + L   D ++                   
Sbjct: 1733 --GGNLCSGGASTAGKRSCLNQLFHVL-ALHMRLYSIDSEYNPWRKLTQLEEMNPQLGYE 1792

Query: 1798 LSRGSDPVLAHDPFASVMWVLFCLPCPFLSCKESLISLVHIFYLVSVAQGIIASCMK-YQ 1857
              +   P+L HD  + ++  +  +P P    K+    +V + + +   Q + A  +K  +
Sbjct: 1793 EQQPEVPILYHDVTSLLLIQILMMPQPLR--KDHFTCIVKVLFTLLYTQALAALSVKCSE 1852

Query: 1858 SKIDGMGFSDCLIADICKIMGESEYARRYFVSD------YTEPSCNVKDMIRSLTFT--Y 1917
                    +  L    C      E    + +S+      Y E       M+  + ++   
Sbjct: 1853 EDRSAWKHAGALKKSTCDAEKSYEVLLSFVISELFKGKLYHEEGTQECAMVNPIAWSPES 1865

Query: 1918 LRRCALLLKLLNSSARAPIFDGETALERYLAVDDM-----IDNIALELNEIGKLEKIFK- 1977
            + +C     L       P     + L+ +L  +D+      +  ++  + +G L   ++ 
Sbjct: 1913 MEKCLQDFCL-------PFLRITSLLQHHLFGEDLPSCQEEEEFSVLASCLGLLPTFYQT 1865

Query: 1978 -------------IPPLDVVLKDEASRSLVWKWFCHFNREFEFQRSK-NLKHCTPAVPFQ 2037
                         +P  D++ +         +      +    Q SK  L H        
Sbjct: 1973 EHPFISASCLDWPVPAFDIITQWCFEIKSFTERHAEQGKALLIQESKWKLPH-------- 1865

BLAST of MS019737 vs. ExPASy Swiss-Prot
Match: F1QJX5 (E3 ubiquitin-protein ligase ubr3 OS=Danio rerio OX=7955 GN=ubr3 PE=2 SV=2)

HSP 1 Score: 219.2 bits (557), Expect = 4.5e-55
Identity = 448/2097 (21.36%), Postives = 789/2097 (37.63%), Query Frame = 0

Query: 93   ESMVWLQWLMFEGEPTFALKNLSKMSFGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVP 152
            E++ W +WL+  G+  F     +  S+    +CG VW  N +AYRCRTC   P  ++C  
Sbjct: 51   EALDWCKWLLAGGD-GFDEFCRTVRSYDNATLCGLVWTANFVAYRCRTCGISPCMSLCAE 110

Query: 153  CFQSGNHKDHDYSIIYT-GGGCCDCGDVTAWKREGFCSKH--KGAERIQPLPEEFVESVG 212
            CF +G+H  HD+++  +  GG CDCGD    +  GFC++H  K  E +  +P + +    
Sbjct: 111  CFNNGDHTGHDFNMFRSQAGGACDCGDGNVMRESGFCNRHRLKTGENVPSVPRDLLLMSE 170

Query: 213  PILDALFTSWKNKLLSAEDISVEDPKLSDRVMEHKKVANELTFAVVEMLLDFCKYSESLL 272
             +L     +    L    D   E    +DR ++  KV  +L    +  L +  K   ++ 
Sbjct: 171  MVLPRFIITIIQYL---RDGYTEPESAADRDLQ--KVLQQLD-PHISFLEELTKMGGAMR 230

Query: 273  SFVSKRVISLAGFLDILVRLERLLTDIVVKKVHELLLKLLGEPIFKYEFAKVFLNYYPTV 332
            + ++K +     F ++ +  E    ++  KK +E  L  L         +   ++     
Sbjct: 231  TVLTKILTDQQTFKELSMGQE---DNVYAKKNYEKYLSALK--------SSGLVSVEEKG 290

Query: 333  ISEAIEEGSDHALKKYPLLPTFSVQIFTVPTLTPRLVEEMNLLSILLGCLEDIFISCVSE 392
             +    +G+  A      L             + +  ++  L  +  G  + + +S  ++
Sbjct: 291  AAGGAGDGTSDAAAGAGALSLLGATATASLDDSSKEEDQDGLQGV--GQRKRVKLSSSTK 350

Query: 393  DGRLQVIKWSNLYETTIRVVEDIRFVMSHAVVPRYVIYEQQDILRTWLRLLTFVQGMDPQ 452
            D  +        +      +E++ F       P+              +++TF+  M P 
Sbjct: 351  DPSIMDTLKHKCF------LEELLFWTIKYEFPQ--------------KMVTFLLNMLPD 410

Query: 453  KRETGLHIEEENENVHLPFGLDHSVANIHTLLVNEAFSAASSSSSSEETADAMSFHAFKQ 512
            +                    D+ +    T + + AF   +   S E  +D MS      
Sbjct: 411  Q--------------------DYKITFTKTFVQHYAFIMKTLMKSHE--SDTMS------ 470

Query: 513  NPDDMDGIRHAKVGRLSQESAACNVFGRSSTVTSASRVDEVCS--DTISSTIMWLTYECL 572
                 + I H  V   S E               A  V E C   D + + ++++   CL
Sbjct: 471  -----NRIVHISVQLFSNEEL-------------ARHVTEECQLLDIMVTVLLYMMESCL 530

Query: 573  KVIDSWLGTEN---------------------VLGGFPNMFAENASMASSCKVYSLR--- 632
              I S L  E                      ++  F N+ +  +      + +SL    
Sbjct: 531  --IKSELQDEENNRHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKRFLEDHSLLLLW 590

Query: 633  -------KPSALESRKISSKVEKGKFIFEKVAGKSKDHNRQYSSRIYSGLQMSIDNEHGV 692
                   +   L  R+++  VE     FE         ++ Y +   + L+       G 
Sbjct: 591  MSFVSFFQGMNLNKRELNEHVE-----FE---------SQTYYAAFAAELEACAQPMWG- 650

Query: 693  SLGEDNQLMDVTNDTVTVEDYSMETDALHFLSFSAWPNIVYDVSSQDISIHIPLHRLLSL 752
             L    ++ +  + T TV  Y +ET  + F +       V + S   ++ H+PLHR  ++
Sbjct: 651  -LLTHCKVKETQDYTKTVVRYCLETLQMWFDAI----GFVDEPSLNQLTFHLPLHRYYAM 710

Query: 753  LLQKALRSSFSESVVPSATGASSSNLSFEYVDFFKSVLTDYHPYGFSAFVMEHPLRIKVF 812
             L K               G     L  +      S+L D         +M HPL+I+  
Sbjct: 711  FLSK---------------GVKCQGLDLD------SLLPDQE---MLMKIMVHPLQIQAS 770

Query: 813  CAEVNAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPELYISRILERF 872
             +E+++ MW RNG         Y    +    ++ D++LLQ CA+   P+ +IS + ERF
Sbjct: 771  LSEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASRLDPDYFISSVFERF 830

Query: 873  RLLDYLSL-----NVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLA 932
            +++D L++     N    SE E  +++  LT ++ + + R   G++  E L+ E++ +L 
Sbjct: 831  KVVDLLTMASQHQNAVLDSEQERPMLEGALTFLVILCSLRIHLGMSDDEILRAEMVSQLC 890

Query: 933  IGDATHSQLVKALPRD-------LSKCHQLQEILDTIAVYSNP-----SGFNQGMYSLRW 992
            + D THS L+  +P +       +      +E+L  +A +  P         QGMY+ + 
Sbjct: 891  MNDRTHSSLLDLIPENPNPKSGVVPGSCSFEEMLSGVADFKAPVFEPGGSMQQGMYTPKA 950

Query: 993  KYW-KELD---LYHPRWSLRDLQIAEERYLRSCGASALTSQLP-----KWTKIYPPFRGL 1052
            + W KE D   +       RD+Q A +RY      S + +  P     + T ++P ++GL
Sbjct: 951  EVWEKEFDPIMVILRTVYRRDVQSAMDRYSAFLKQSGVHTGNPWPPYKERTPLHPCYKGL 1010

Query: 1053 ARIATCKAVLQIIRAVLFYSVFTDKSTKSRAPDSVLLTALHLLALALDICFQRKESSDQS 1112
             R+  CK  L I+   L Y ++ D    S   + VL   L+L+ L LD   Q  +  ++ 
Sbjct: 1011 VRLLHCK-TLHIVIFTLLYKIWMDHQNMS---EHVLCMVLYLIELGLDNQVQ-DDKVEEE 1070

Query: 1113 FCSEDSIPLLLFADEEVDEGLTYGLG--RQSLLSLLILLMKMHKKEGR------------ 1172
             C E+      F    +   L + +   R  +      + +  ++E              
Sbjct: 1071 PCIEEHCHDSWFPGTSLLSNLHHVINFVRVRVPETAPEVERKRERERERETPPSTSSESA 1130

Query: 1173 ---ENLLEAGSCNLSSLVESLLKKFSEIDSRCMGKIRQHAPEILGYLSQSLPSSDPNSLS 1232
               +NL EA    + SLV    +KF EI +R   +  Q A       S+ LP   P  L 
Sbjct: 1131 TFGQNLREA---QVFSLVAERRRKFQEIINRSNHEASQ-AVRPKSSASRWLPPGTPPQL- 1190

Query: 1233 ETSDSDKLKAKARERQAAILEKMRAEQ----YKFLASVDSSVEDDAEFGQESEKPSVSDS 1292
              ++  +++         + +K+ A+Q      +LA VD +     +     E+     S
Sbjct: 1191 -VTEILEIRESMLSLLVKLHQKLSAKQNSLSLSWLAEVDPAHHAHGDGLTAIERILAKAS 1250

Query: 1293 AEQSET---VCSLCHDSSSSVPISYLILLQKSKLVSLIDRGTVSWDQPYWRDEHASSIPR 1352
            A    +   +  +C      +P          K +S  D+ ++  ++            R
Sbjct: 1251 ARSRHSKRCLQEICGKVCPPIP---------PKKISPGDKKSMDKEE------------R 1310

Query: 1353 RNLDQSGLSTSSGGSGGISSPQLAELIQNAVKEYASHGLPGEVGAFLDFVKSHFPPLRNI 1412
            R                    +  E  Q  + E+AS              KS      ++
Sbjct: 1311 RQ-------------------RARERQQKLLAEFASR------------QKSFMETAMDV 1370

Query: 1413 QVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHENMHSKLNEDENFSKV----ASSLVTE 1472
            + P    V     + S ++L+ ++       +         ED     V    ASS++  
Sbjct: 1371 ESPEADAVMD---VSSEESLDSEVLYDC---VICGQSGPSTEDRPTGLVVLLQASSVLGH 1430

Query: 1473 DSKSVLHVKYIAALSRELAENHSTSESAHNVSMPVESLQPTTFNEIDPVDC------DGI 1532
              +S +  K +     E      T  + H+V +   SL    F +   +         G+
Sbjct: 1431 RCRSDM-PKRLPTTDEEHIYPEDTCGATHDVRL---SLMQRYFKDSSCLQSVSIGWDGGV 1490

Query: 1533 YLSSCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAF 1592
            Y+ +CGH +H  C   Y+ SL+       V +G   +  ++GEF CP+CR+ ANS LP  
Sbjct: 1491 YVQTCGHTLHIDCHKSYMESLR----NDQVLQG---ISVDKGEFTCPLCRQFANSVLPCR 1550

Query: 1593 PREFQKFGNPSMSSIGSLSHVSGQSNKSTEEVNPLYVQKAVAFLQSTAKAVGKNKVLKDI 1652
            P    + G     S  S+S +        +EV  L  Q  +  ++++ K   ++ +L  I
Sbjct: 1551 PGRGMETGAWHAPSTKSMSTL-------VKEVEDLQEQLGIFPVRASIKQ--RDPIL--I 1610

Query: 1653 SVHRHRKVSRNLEAVSLVLSKLYFS-GKQDKSISCTRVNPSILMWDTLKYSLVSMEIAAR 1712
              +  +++   ++ +     K Y   GK   S      N  + M+               
Sbjct: 1611 ESNLSKEMESVIKDIKNTTQKKYMDYGKNPGSPD----NDFLFMY--------------- 1670

Query: 1713 SKTDVNPNIGLNTLYKELKTSGGFILSLLLKVIQSIKCEDSLLLLQRFCGIQRFADSICS 1772
                   ++    L  EL   GG                                 ++CS
Sbjct: 1671 -------SVARTNLELELVHRGG---------------------------------NLCS 1730

Query: 1773 GVSNENANDSCGQGILHILTSLRVELPQFDIQF-------------------LSRGSDPV 1832
            G ++  A  SC   + H+L ++ + L   D  +                     R   P+
Sbjct: 1731 GGASAAAKRSCLNQLFHVL-AMHMRLYSIDSAYNPWTRLTLSTQSRENEYCDEERPEVPM 1790

Query: 1833 LAHDPFASVMWVLFCLPCPFLSCKESLISLVHIFYLVSVAQGIIASCMKYQSKIDGMGFS 1892
            L  D     + ++F L  P    KE    +V + Y +   Q + A  +++ S+ + + +S
Sbjct: 1791 LFRD--VPSLLIIFILTMPQPLRKEHFTCVVKVLYSLQFTQALAALSIRF-SREERLAWS 1840

Query: 1893 DCLIAD-------------ICKIMGESEYARRYFVSDYTEPSC---------NVKDMIRS 1952
            +   A              +  ++ E   A+  + ++  E S          +++  ++ 
Sbjct: 1851 NTGAAKKNLPNSDKSWESLLGHMISELTKAKDVYDTNSEETSALSSSVWSPQSIEFRLQQ 1840

Query: 1953 LTFTYLR-RCALLLKLLNSSARAPIFDGE-TALERYLAV-------DDMIDNIALELNEI 2012
                +LR  C L   L   S    + + E + L R L +         M     LE N I
Sbjct: 1911 FCLPFLRLSCLLQHHLYGDSLPGCLVEEEFSLLTRCLGLAASVQCSGSMSSAACLEWN-I 1840

Query: 2013 GKLEKIFKIPPLDVVLKDEASRSLVWKWFCHFNREFEFQRSKNLKHCTP--AVPFQLIRL 2037
               + I +     V L D  S                 Q+S +L    P  A P +L++L
Sbjct: 1971 SAFDLISQWCSEVVALSDTPS-----------------QQSASLLGQDPQWAAP-RLLQL 1840

BLAST of MS019737 vs. ExPASy Swiss-Prot
Match: Q5U430 (E3 ubiquitin-protein ligase UBR3 OS=Mus musculus OX=10090 GN=Ubr3 PE=1 SV=3)

HSP 1 Score: 216.9 bits (551), Expect = 2.2e-54
Identity = 426/2041 (20.87%), Postives = 758/2041 (37.14%), Query Frame = 0

Query: 118  SFGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYT-GGGCCDC 177
            ++    +CG VW  N +AYRCRTC   P  ++C  CF  G+H  HD+++  +  GG CDC
Sbjct: 113  AYDPAALCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGDHTGHDFNMFRSQAGGACDC 172

Query: 178  GDVTAWKREGFCSKH--KGAERIQPLPEEFV---ESVGPILDALFTSWKNKLLSAEDISV 237
            GD    +  GFC +H  K +  I  +P++ +   E V P        +  +  +      
Sbjct: 173  GDSNVMRESGFCRRHQIKSSSNIPCVPKDLLMMSEFVLPRFIFCLIQYLREGYNEPAADA 232

Query: 238  EDPKLSDRVMEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISLAGFLDILVRLER 297
               K  ++V+       +L    +  L D  K   ++ S +++ + +   + D+   L  
Sbjct: 233  PSEKDLNKVL-------QLLEPQISFLEDLTKMGGAMRSVLTQVLTNQQNYKDLTAGLGE 292

Query: 298  LLTDIVVKKVHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHALKKYPLLPTF 357
               +   KK HE  L  L      Y   K+           +                T 
Sbjct: 293  ---NACAKKSHEKYLIALKSSGLTYPEDKLVYGVQEPAAGTS----------------TL 352

Query: 358  SVQIFTVPTLTPRLV----EEMNLLSILLGCLEDIFISCVSED-GRLQVIKWSNLYETTI 417
            + Q F   T T   +    EE    S  LG  + + +S  ++D   + V+K  +      
Sbjct: 353  AAQGFAGATGTLGQIDSSDEEDQDGSQGLGKRKRVKLSSGTKDQSIMDVLKHKSF----- 412

Query: 418  RVVEDIRFVMSHAVVPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHL 477
              +E++ F       P+              +++TF+  M P +                
Sbjct: 413  --LEELLFWTIKYEFPQ--------------KMVTFLLNMLPDQ---------------- 472

Query: 478  PFGLDHSVANIHTLLVNEAFSAASSSSSSEETADAMSFHAFKQNPDDMDGIRHAKVGRLS 537
                ++ VA   T + + AF   +   S E  +D MS           + I H  V   S
Sbjct: 473  ----EYKVAFTKTFVQHYAFIMKTLKKSHE--SDTMS-----------NRIVHISVQLFS 532

Query: 538  QESAACNVFGRSSTVTSASRVDEVCS--DTISSTIMWLTYECLKVIDSWLGTENVLGGFP 597
             E               A +V E C   D + + ++++   CL +       EN L    
Sbjct: 533  NEEL-------------ARQVTEECQLLDIMVTVLLYMMESCL-IKSELQDEENSLHVVV 592

Query: 598  N----MFAENASMASSCKVYSLRKPSALESRKISSK---VEKGKFI--FEKVAGKSKDHN 657
            N    +   N          ++    ++  R +      V    F+  F+ +    ++ N
Sbjct: 593  NCGEALLKNNTYWPLVSDFINILSHQSVAQRFLEDHGLLVTWMNFVSFFQGMNLNKRELN 652

Query: 658  R--QYSSRIY---SGLQMSIDNEHGVSLGEDNQLMDVTNDTVTVEDYSMET-----DALH 717
               ++ S+ Y      ++    +    L    ++ +    T  V  Y +E      DA++
Sbjct: 653  EHVEFESQTYYAAFAAELEACAQPMWGLLSHCKVRETQEYTRNVVRYCLEALQDWFDAIN 712

Query: 718  FLSFSAWPNIVYDVSSQDISIHIPLHRLLSLLLQKALRSSFSESVVPSATGASSSNLSFE 777
            F+   A PN         ++ H+PLHR  ++ L KA++    +                 
Sbjct: 713  FVDEPA-PN--------QVTFHLPLHRYYAMFLSKAVKCQELD----------------- 772

Query: 778  YVDFFKSVLTDYHPYGFSAFVMEHPLRIKVFCAEVNAGMWRRNGDAALLSCELYRSIRWS 837
                  S+L D         +M HPL+I+   AE+++ MW RNG         Y    + 
Sbjct: 773  ----LDSLLPDQE---MLMKLMIHPLQIQASLAEIHSNMWVRNGLQIKGQAMTYVQSHFC 832

Query: 838  EQCLELDLFLLQCCAAMAPPELYISRILERFRLLDYLSL-----NVERPSEYEPILVQEM 897
               ++ D++LLQ CA+   P+ +IS + ERF+++D L++     N+    E+E  +++  
Sbjct: 833  NSMIDPDIYLLQVCASRLDPDYFISSVFERFKVVDLLTMASQHHNMVLDVEHERSMLEGA 892

Query: 898  LTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQLVKALPRD-------LSKCHQ 957
            LT ++ +++ R   G++  + L+ E++ +L + D THS L+  +P +       +   + 
Sbjct: 893  LTFLVILLSLRLHLGMSDDDILRAEMVAQLCMNDRTHSSLLDLIPENPNPKSGIIPGSYS 952

Query: 958  LQEILDTIAVYSNP-----SGFNQGMYSLRWKYW-KELD---LYHPRWSLRDLQIAEERY 1017
             + +L  +A +  P         QGMY+ + + W +E D   +       RD+Q A +RY
Sbjct: 953  FESVLSAVADFRAPIFEPGGSMQQGMYTPKAEVWDQEFDPVMVILRTVYRRDVQSAMDRY 1012

Query: 1018 ---LRSCGASALTSQLP--KWTKIYPPFRGLARIATCKAVLQIIRAVLFYSVFTDKSTKS 1077
               L+  G        P  K T ++P ++GL R+  CK  L I+   L Y +  D    S
Sbjct: 1013 TAFLKQSGKFPGNPWPPYKKRTSLHPSYKGLMRLLHCK-TLHIVLFTLLYKILMDHQNLS 1072

Query: 1078 RAPDSVLLTALHLLALALDICFQRKESSDQSFCSEDSIPLLLFADEEVDEGLTYGLG--- 1137
               + VL   L+L+ L L+         + S    +      F    +   + + +    
Sbjct: 1073 ---EHVLCMVLYLIELGLENSADDDSEEEVSMGGPERCHDSWFPGSNLVSNMRHFINYVR 1132

Query: 1138 ---RQSLLSLLILLMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDSRCMGKIRQHA 1197
                ++   L    +     +  ++L  +G+  + SLV    KKF EI +R   +  Q  
Sbjct: 1133 VRVPETAPELKRDPLASTSSDALDSLQNSGTAQVFSLVAERRKKFQEIINRSNSEANQVV 1192

Query: 1198 -PEILGYLSQSLPSSDPNSLSETSDSDKLKAKARERQAAILEKMRAEQYKFLASVDSSVE 1257
             P+I            P+  S    S +L     E + +IL  +    +K     +S   
Sbjct: 1193 RPKI------------PSKWSAPGSSPQLTTAILEIKESILSLLIKLHHKLSGKQNSYYP 1252

Query: 1258 ---DDAEFGQESEKPSVSDSAEQSETVCSLCHDSSSSVPISYLILLQKSKLVSLIDRGTV 1317
               DD E   + E P  +              D  ++V    L    +S++   I     
Sbjct: 1253 PWLDDIEVLIQPEIPKYNHG------------DGITAVERILLKAAVQSRMNKRIIEEIC 1312

Query: 1318 SWDQPYWRDEHASSIPRRNLDQSGLSTSSGGSGGISSPQLAELIQNAVKEYASHGLPGEV 1377
                P    +  ++  ++ LD+                +  E  Q  + E+AS       
Sbjct: 1313 RKVTPPVPPKKITAAEKKTLDKEE-----------RRQKARERQQKLLAEFASR------ 1372

Query: 1378 GAFLDFVKSHFPPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHENMHSKLNED 1437
                   KS      ++  P         I     T E  +  +V   +        +ED
Sbjct: 1373 ------QKSFMETAMDVDSPEND------IPMEITTAEPQVSEAVYDCVICGQSGPSSED 1432

Query: 1438 ENFSKVA---SSLVTEDSKSVLHVKYIAALSRELAENHSTSESAHNVSMPVESLQPTTFN 1497
                 V    +S V    +     K +     E      T  + H+V +   SL    F 
Sbjct: 1433 RPTGLVVLLQASSVLGQCRDNAEPKKLPIAEEEQIYPWDTCAAVHDVRL---SLLQRYFK 1492

Query: 1498 EIDPVDC------DGIYLSSCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEF 1557
            +   +         G+Y+ +CGH +H  C   Y+ SL+       V +G  +   ++GEF
Sbjct: 1493 DSSCLLAVSIGWEGGVYVQTCGHTLHIDCHKSYMESLR----NDQVLQGFSV---DKGEF 1552

Query: 1558 LCPVCRRLANSTLPAFPREFQKFGNPSMSSIGSLSHVSGQSNKSTEEVNPLYVQKAVAFL 1617
             CP+CR+ ANS LP +P      G+   +++          NKST++     + K V  L
Sbjct: 1553 TCPLCRQFANSVLPCYP------GSNVENNLW-----QRPCNKSTQD-----LIKEVEEL 1612

Query: 1618 QSTAKAVGKNKVLKDISVHRHRKVSRNLEAVSLVLSKLYFSGKQDKSISCTRVNPSILMW 1677
            Q    A                 +S+ +E+V              K I  T         
Sbjct: 1613 QGRPGAFPS-----------ETNLSKEMESVM-------------KDIKNT--------- 1672

Query: 1678 DTLKYSLVSMEIAARSKTDVNPNIGLNTLYKELKTSGGFILSLLLKVIQSIKCEDSLLLL 1737
                      +    SKT  +P+     +Y   +T      +L L++I            
Sbjct: 1673 -------TQKKYRDYSKTPGSPDNEFLFMYSVART------NLELELIHR---------- 1732

Query: 1738 QRFCGIQRFADSICSGVSNENANDSCGQGILHILTSLRVELPQFDIQFL----------- 1797
                       S+CSG  +     SC   + H+L +L + L   D ++            
Sbjct: 1733 ---------GGSLCSGGPSTAGKRSCLNQLFHVL-ALHMRLYTIDSEYNPWKKLTQLVED 1792

Query: 1798 ---------SRGSDPVLAHDPFASVMWVLFCLPCPFLSCKESLISLVHIFYLVSVAQGII 1857
                      +   P+L HD  + ++  +  +P P    KE    +V + + +   Q + 
Sbjct: 1793 MNSQVGNEDQQPEVPILYHDVTSLLLIQILMMPQPLR--KEHFTCIVKVLFTLLYTQALA 1852

Query: 1858 ASCMK-YQSKIDGMGFSDCLIADICKIMGESEYARRYFVSDYTEPSCNVKDMIRSLTFTY 1917
            A  +K  +        +  L  D C      E    + +S+ ++     ++         
Sbjct: 1853 ALSVKGTEEDRSAWKHAGALRKDTCDAEKCYEVLLSFVISELSKGKLYYEE--------G 1866

Query: 1918 LRRCALLLKLLNSSAR---------APIFDGETALERYLAVDDM-----IDNIALELNEI 1977
             + CA++  +  S             P     + L+ +L  +D+      +  ++  + +
Sbjct: 1913 AQECAMVSPIAWSPESMERYIQDFCLPFLRVSSLLQHHLFGEDLPSCQEEEEFSVLASCL 1866

Query: 1978 GKLEKIFKI--PPLDVVLKD--EASRSLVWKWFCHF----NREFEFQRSKNLKHCTPAVP 2037
            G L   ++   P +     D   A+  ++ +W         R  E  ++  ++     +P
Sbjct: 1973 GLLPTFYQTDHPFISASCLDWPVAAFDIITQWCFEITSFTERHAEQGKALLIQESRWKLP 1866

BLAST of MS019737 vs. ExPASy Swiss-Prot
Match: Q8IWV7 (E3 ubiquitin-protein ligase UBR1 OS=Homo sapiens OX=9606 GN=UBR1 PE=1 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 1.1e-53
Identity = 437/2103 (20.78%), Postives = 756/2103 (35.95%), Query Frame = 0

Query: 98   LQWLMFEGEPTFALKNLSKMSFGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSG 157
            L+W +F  +P   L+ L     G   +CG V+   +  Y CR C  DPTC +C+ CFQ  
Sbjct: 74   LEWYLFGEDPDICLEKLKHS--GAFQLCGRVFKSGETTYSCRDCAIDPTCVLCMDCFQDS 133

Query: 158  NHKDHDYSI-IYTGGGCCDCGDVTAWKREGFCSKHK--GAERIQ-----PLPEEFVESVG 217
             HK+H Y +   TGGG CDCGD  AWK   FC  H+   A  I+     PL EE +    
Sbjct: 134  VHKNHRYKMHTSTGGGFCDCGDTEAWKTGPFCVNHEPGRAGTIKENSRCPLNEEVIVQAR 193

Query: 218  PILDAL------FTSWKNK-------------------LLSAEDISVE----------DP 277
             I  ++       T W+ +                   L + E  S +          D 
Sbjct: 194  KIFPSVIKYVVEMTIWEEEKELPPELQIREKNERYYCVLFNDEHHSYDHVIYSLQRALDC 253

Query: 278  KLSDRVMEHKKVANELTFAV----------------------------VEML----LDFC 337
            +L++  +    +  E   AV                            VE+L    +   
Sbjct: 254  ELAEAQLHTTAIDKEGRRAVKAGAYAACQEAKEDIKSHSENVSQHPLHVEVLHSEIMAHQ 313

Query: 338  KYSESLLSFVSKRVISLAGFLDILVR--------------LERL-LTDIVVKK-----VH 397
            K++  L S+++K +   + F  I  +              + RL L D  + K     +H
Sbjct: 314  KFALRLGSWMNKIMSYSSDFRQIFCQACLREEPDSENPCLISRLMLWDAKLYKGARKILH 373

Query: 398  ELLL-KLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHALKKYPLLPTFSVQIFTVPTL 457
            EL+      E  +K  FA  F+ YY  +  E I +  D ++     +   SVQ+FTVPTL
Sbjct: 374  ELIFSSFFMEMEYKKLFAMEFVKYYKQLQKEYISDDHDRSIS----ITALSVQMFTVPTL 433

Query: 458  TPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWS-NLYETTIRVVEDIRFVMSHAV 517
               L+EE N++S++   L ++    +  + +     +S +       V+ D+++++    
Sbjct: 434  ARHLIEEQNVISVITETLLEVLPEYLDRNNKFNFQGYSQDKLGRVYAVICDLKYIL---- 493

Query: 518  VPRYVIYEQQ------DILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSV 577
            + +  I+ ++      +  R++L++LT +QGM+  +R+ G HIE + +            
Sbjct: 494  ISKPTIWTERLRMQFLEGFRSFLKILTCMQGMEEIRRQVGQHIEVDPD------------ 553

Query: 578  ANIHTLLVNEAFSAASSSSSSEETADAMSFHAFKQNPDDMDGIRHAKVGRLSQESAACNV 637
                                  E A A+              ++   +  + QE  AC+ 
Sbjct: 554  ---------------------WEAAIAIQ-------------MQLKNILLMFQEWCACD- 613

Query: 638  FGRSSTVTSASRVDEVCSDTISSTIMWLTY-ECLKVIDSWLGTENVLGGFPNMFAENASM 697
                                    ++ + Y EC K          V+    +  + + ++
Sbjct: 614  ----------------------EELLLVAYKECHKA---------VMRCSTSFISSSKTV 673

Query: 698  ASSCKVYSLRKPSALESRKISSKVEKGKFIFEKVAGKSKDHNRQYSSRIYSGLQMSIDNE 757
              SC                                                        
Sbjct: 674  VQSC-------------------------------------------------------- 733

Query: 758  HGVSLGEDNQLMDVTNDTVTVEDYSMETDALHFLSFSAWPNIVYDVSSQDISIHIPLHRL 817
                                   +S+ET +             Y VS   +SIH+PL R 
Sbjct: 734  ----------------------GHSLETKS-------------YRVSEDLVSIHLPLSRT 793

Query: 818  LSLLLQKALRSSFSESVVPSATGASSSNLSFEYVDFFKSVLTDYHPYGFSAFVMEHPLRI 877
            L+ L  +  R          A       +SFE  DF   VL +Y            PLR 
Sbjct: 794  LAGLHVRLSRL--------GAVSRLHEFVSFE--DFQVEVLVEY------------PLRC 853

Query: 878  KVFCAEVNAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPELYISRIL 937
             V  A+V A MWRRNG + +     Y+ ++  E+  + D+ +LQ  A++  P  ++  +L
Sbjct: 854  LVLVAQVVAEMWRRNGLSLISQVFYYQDVKCREEMYDKDIIMLQIGASLMDPNKFLLLVL 913

Query: 938  ERFRLLDYLSLNVERPSE----YEPILVQEMLTLIIQVVNERRFCG---LTVAESLKREL 997
            +R+ L +  +  +    +        L++EML ++I +V ER   G   +T  E   RE+
Sbjct: 914  QRYELAEAFNKTISTKDQDLIKQYNTLIEEMLQVLIYIVGERYVPGVGNVTKEEVTMREI 973

Query: 998  IYKLAIGDATHSQLVKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLRWKYWKELD 1057
            I+ L I    HS + K LP + +    L+ +++ +A +  P     G+Y L+ +  K+ +
Sbjct: 974  IHLLCIEPMPHSAIAKNLPENENNETGLENVINKVATFKKPGVSGHGVYELKDESLKDFN 1033

Query: 1058 LYHPRWSLRDLQIAEERYLRSCGASALTSQL--PKWTKIYPPFRGLARIATCKAVLQIIR 1117
            +Y   +S      AE    +          L  P   +  P F  +  +  C  ++ I+R
Sbjct: 1034 MYFYHYSKTQHSKAEHMQKKRRKQENKDEALPPPPPPEFCPAFSKVINLLNCDIMMYILR 1093

Query: 1118 AVLFYSVFTDKSTKSRAPDSVLLTALHLLALALDICFQRKESSDQSFCSEDSIPLLLFAD 1177
             V   ++ TD +  +   + +L  A H+LAL L                E+   L    +
Sbjct: 1094 TVFERAIDTDSNLWT---EGMLQMAFHILALGL---------------LEEKQQLQKAPE 1153

Query: 1178 EEVDEGLTYGLGR--QSLLSLLILLMKMH---KKEGRENLLEAGSCNLSSLVESLLKKFS 1237
            EEV     +   R   S +++ +LL K+    + EG+++++      +   V+ L +K  
Sbjct: 1154 EEVTFDFYHKASRLGSSAMNIQMLLEKLKGIPQLEGQKDMI-TWILQMFDTVKRLREKSC 1213

Query: 1238 EIDSRCMGKIRQHAPEILGYLSQSLPSSDPNSLSETSDSDKLKAKARERQAAILEKMRAE 1297
             I +   G             S+S+ + +     E ++  +    AR  +  I+ +M A 
Sbjct: 1214 LIVATTSG-------------SESIKNDEITHDKEKAERKRKAEAARLHRQKIMAQMSAL 1273

Query: 1298 QYKFLASVDSSVEDDAEFGQESEKPSVSDSAEQSETVCSLCHDSSSSVPISYLILLQKSK 1357
            Q  F+ +          +   SE P   DS  + E                         
Sbjct: 1274 QKNFIET------HKLMYDNTSEMPGKEDSIMEEE------------------------- 1333

Query: 1358 LVSLIDRGTVSWDQPYWRDEHASSIPRRNLDQSGLSTSSGGSGGISSPQLAELIQNAVKE 1417
                                                         S+P        AV +
Sbjct: 1334 ---------------------------------------------STP--------AVSD 1393

Query: 1418 YASHGLPGEVGAFLDFVKSHFPPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMH 1477
            Y+   L  + G                  P  +    EK + +    +E+  + +     
Sbjct: 1394 YSRIALGPKRG------------------PSVT----EKEVLTCILCQEEQEVKI----- 1453

Query: 1478 ENMHSKLNEDENFSKVASSLVTEDSKSVLHVKYIAALSRELAENHSTSESAHNVSMPVES 1537
                      EN + V S+ V + +    H                       + +  E+
Sbjct: 1454 ----------ENNAMVLSACVQKSTALTQH-------------------RGKPIELSGEA 1513

Query: 1538 LQPTTFNEIDPVDCDGIYLSSCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGE 1597
            L P     +DP    G Y  SCGH +H  C  +Y  +++    +RI  +   + D E GE
Sbjct: 1514 LDPLF---MDPDLAYGTYTGSCGHVMHAVCWQKYFEAVQLSSQQRIHVD---LFDLESGE 1573

Query: 1598 FLCPVCRRLANSTLPAFPREFQKFGNPSMSSIGS-----------LSHVSGQSNKSTEEV 1657
            +LCP+C+ L N+ +P  P + QK  + +  ++             L+ +SG + +  +  
Sbjct: 1574 YLCPLCKSLCNTVIPIIPLQPQKINSENADALAQLLTLARWIQTVLARISGYNIRHAKGE 1633

Query: 1658 NPLYVQKAVAFLQSTAKAVGKNKVLKDISVHRHRKVSRNL-EAVSLVLSKLYFSG-KQDK 1717
            NP+     + F Q    +  +   +    V    K S ++ E V L  + +Y  G K   
Sbjct: 1634 NPI----PIFFNQGMGDSTLEFHSILSFGVESSIKYSNSIKEMVILFATTIYRIGLKVPP 1693

Query: 1718 SISCTRVNPSILMWDTLKYSLVSMEIAARSKTDVNPNIGLNTLYKELKTSGGFILSLLLK 1777
                 RV   +L W T  +++ ++E               N L  E K   G + +    
Sbjct: 1694 DERDPRV--PMLTWSTCAFTIQAIE---------------NLLGDEGKPLFGALQN---- 1718

Query: 1778 VIQSIKCEDSLLLLQRFCGIQRFADSICSGVSNENANDSCGQGIL--HILTSLRVELPQF 1837
                 +  + L  L +F   QR                +C Q ++  H++  L V LP  
Sbjct: 1754 -----RQHNGLKALMQFAVAQRI---------------TCPQVLIQKHLVRLLSVVLPNI 1718

Query: 1838 DIQFLSRGSDPVLAHDPFASVMWVLFCLPCPFL--------SCKESLISLVHIFYLVSVA 1897
                 S  +  +L+ D F  ++  +   P  +         S   S  + +++F+L+++A
Sbjct: 1814 K----SEDTPCLLSIDLFHVLVGAVLAFPSLYWDDPVDLQPSSVSSSYNHLYLFHLITMA 1718

Query: 1898 QGIIASCMKYQSKIDGMGFSDCLIADICKIMGESEYARRYF--VSDYTEPS--CNVKD-- 1957
                   ++    +D    +   +A + +   E+  A  +F  +S YT  S  C++    
Sbjct: 1874 H-----MLQILLTVD----TGLPLAQVQEDSEEAHSASSFFAEISQYTSGSIGCDIPGWY 1718

Query: 1958 ---MIRSLTFTYLRRCALLLKLLNSSARAPIFDGETALERYLAVDDMIDNIALELNEIGK 2017
                +++    YLR  AL    L            +A   Y A   +   ++L  N    
Sbjct: 1934 LWVSLKNGITPYLRCAALFFHYLLGVTPPEELHTNSAEGEYSA---LCSYLSLPTNLFLL 1718

Query: 2018 LEKIFKIPPLDVVLKDEASRSLVWKWFCHFNREFEFQRSKNLKHCTPAVPFQLIRLPHVY 2037
             ++ +           +  R L+ +W C         + KN     P     LI LP  Y
Sbjct: 1994 FQEYW-----------DTVRPLLQRW-CADPALLNCLKQKNTVVRYPRKRNSLIELPDDY 1718

BLAST of MS019737 vs. ExPASy TrEMBL
Match: A0A6J1CUV9 (E3 ubiquitin-protein ligase OS=Momordica charantia OX=3673 GN=LOC111014998 PE=3 SV=1)

HSP 1 Score: 4013.0 bits (10406), Expect = 0.0e+00
Identity = 2029/2040 (99.46%), Postives = 2033/2040 (99.66%), Query Frame = 0

Query: 1    MDDMDIGSPSESAPLKPRDRIVRRLALLGVPEELLDQLQPGLVSFVKDNKILIPQLVSAI 60
            MDDM+IGSPSESAPLKPRDRIVRRLALLGVPEELLDQLQPGLVSFVKDNKILIPQLVSAI
Sbjct: 1    MDDMNIGSPSESAPLKPRDRIVRRLALLGVPEELLDQLQPGLVSFVKDNKILIPQLVSAI 60

Query: 61   LPTDVEVAEVIRDAKAGSKKSMAGPTMKNQFRESMVWLQWLMFEGEPTFALKNLSKMSFG 120
            LPTDVEVAEVIRDAKAGSKKS+AGPTMKNQFRESMVWLQWLMFEGEPTFALKNLSKMSFG
Sbjct: 61   LPTDVEVAEVIRDAKAGSKKSVAGPTMKNQFRESMVWLQWLMFEGEPTFALKNLSKMSFG 120

Query: 121  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVT 180
            QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVT
Sbjct: 121  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVT 180

Query: 181  AWKREGFCSKHKGAERIQPLPEEFVESVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 240
            AWKREGFCSKHKGAERIQPLPEEFVESVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV
Sbjct: 181  AWKREGFCSKHKGAERIQPLPEEFVESVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 240

Query: 241  MEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISLAGFLDILVRLERLLTDIVVKK 300
            MEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISLAGFLDILVRLERLLTDIVVKK
Sbjct: 241  MEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISLAGFLDILVRLERLLTDIVVKK 300

Query: 301  VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHALKKYPLLPTFSVQIFTVPT 360
            VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHALKKYPLLPTFSVQIFTVPT
Sbjct: 301  VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHALKKYPLLPTFSVQIFTVPT 360

Query: 361  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAV 420
            LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAV
Sbjct: 361  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAV 420

Query: 421  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHTL 480
            VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHTL
Sbjct: 421  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHTL 480

Query: 481  LVNEAFSAASSSSSSEETADAMSFHAFKQNPDDMDGIRHAKVGRLSQESAACNVFGRSST 540
            LVNEAFSAASSSSSSEETADAMSFHAFKQNPDDMD IRHAKVGRLSQESAACNVFGRSST
Sbjct: 481  LVNEAFSAASSSSSSEETADAMSFHAFKQNPDDMDSIRHAKVGRLSQESAACNVFGRSST 540

Query: 541  VTSASRVDEVCSDTISSTIMWLTYECLKVIDSWLGTENVLGGFPNMFAENASMASSCKVY 600
            VTSASRVDEVCSDTISSTIMWLTYECLKVIDSWLGTEN LGGFPNMFAENASMASSCKVY
Sbjct: 541  VTSASRVDEVCSDTISSTIMWLTYECLKVIDSWLGTENALGGFPNMFAENASMASSCKVY 600

Query: 601  SLRKPSALESRKISSKVEKGKFIFEKVAGKSKDHNRQYSSRIYSGLQMSIDNEHGVSLGE 660
            SLRKPSALESRKISSKVEKGKFIFEKVAGKSKDHNRQYSSRIYSGLQMSIDNEHGVSLGE
Sbjct: 601  SLRKPSALESRKISSKVEKGKFIFEKVAGKSKDHNRQYSSRIYSGLQMSIDNEHGVSLGE 660

Query: 661  DNQLMDVTNDTVTVEDYSMETDALHFLSFSAWPNIVYDVSSQDISIHIPLHRLLSLLLQK 720
            DNQLMDVTNDTVTVEDY+METDALHFLSFSAWPNIVYDVSSQDISIHIPLHRLLSLLLQK
Sbjct: 661  DNQLMDVTNDTVTVEDYAMETDALHFLSFSAWPNIVYDVSSQDISIHIPLHRLLSLLLQK 720

Query: 721  ALRSSFSESVVPSATGASSSNLSFEYVDFFKSVLTDYHPYGFSAFVMEHPLRIKVFCAEV 780
            ALRSSFSESVVPSATGASSSNLSFEYVDFFKSVLTDYHPYGFSAFVMEHPLRIKVFCAEV
Sbjct: 721  ALRSSFSESVVPSATGASSSNLSFEYVDFFKSVLTDYHPYGFSAFVMEHPLRIKVFCAEV 780

Query: 781  NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPELYISRILERFRLLD 840
            NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPELYISRILERFRLLD
Sbjct: 781  NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPELYISRILERFRLLD 840

Query: 841  YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQL 900
            YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQL
Sbjct: 841  YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQL 900

Query: 901  VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLRWKYWKELDLYHPRWSLRDLQIA 960
            VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLRWKYWKELDLYHPRWSLRDLQIA
Sbjct: 901  VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLRWKYWKELDLYHPRWSLRDLQIA 960

Query: 961  EERYLRSCGASALTSQLPKWTKIYPPFRGLARIATCKAVLQIIRAVLFYSVFTDKSTKSR 1020
            EERYLRSCGASALTSQLPKWTKIYPPFRGLARIATCKAVLQIIRAVLFYSVFTDKSTKSR
Sbjct: 961  EERYLRSCGASALTSQLPKWTKIYPPFRGLARIATCKAVLQIIRAVLFYSVFTDKSTKSR 1020

Query: 1021 APDSVLLTALHLLALALDICFQRKESSDQSFCSEDSIPLLLFADEEVDEGLTYGLGRQSL 1080
            APDSVLLTALHLLALALDICFQRKESSDQSFCSEDSIPLLLFADEEVDEGLTYGLGRQSL
Sbjct: 1021 APDSVLLTALHLLALALDICFQRKESSDQSFCSEDSIPLLLFADEEVDEGLTYGLGRQSL 1080

Query: 1081 LSLLILLMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDSRCMGKIRQHAPEILGYL 1140
            LSLLILLMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDSRCMGKIRQHAPEILGYL
Sbjct: 1081 LSLLILLMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDSRCMGKIRQHAPEILGYL 1140

Query: 1141 SQSLPSSDPNSLSETSDSDKLKAKARERQAAILEKMRAEQYKFLASVDSSVEDDAEFGQE 1200
            SQSLPSSDPNSLSETSDSDKLKAKARERQAAILEKMRAEQYKFLASVDSSVEDDAEFGQE
Sbjct: 1141 SQSLPSSDPNSLSETSDSDKLKAKARERQAAILEKMRAEQYKFLASVDSSVEDDAEFGQE 1200

Query: 1201 SEKPSVSDSAEQSETVCSLCHDSSSSVPISYLILLQKSKLVSLIDRGTVSWDQPYWRDEH 1260
            SEKPSVSDSAEQSETVCSLCHDSSSSVPISYLILLQKSKLVSLIDRGTVSWDQPYWRDEH
Sbjct: 1201 SEKPSVSDSAEQSETVCSLCHDSSSSVPISYLILLQKSKLVSLIDRGTVSWDQPYWRDEH 1260

Query: 1261 ASSIPRRNLDQSGLSTSSGGSGGISSPQLAELIQNAVKEYASHGLPGEVGAFLDFVKSHF 1320
            ASSIPRRNLDQS LSTS   SGGISSPQLA+LIQNAVKEYASHGLPGEVGAFLDFVKSHF
Sbjct: 1261 ASSIPRRNLDQSELSTS---SGGISSPQLAQLIQNAVKEYASHGLPGEVGAFLDFVKSHF 1320

Query: 1321 PPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHENMHSKLNEDENFSKVASSLV 1380
            PPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHENMHSKLNEDEN SKVASSLV
Sbjct: 1321 PPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHENMHSKLNEDENLSKVASSLV 1380

Query: 1381 TEDSKSVLHVKYIAALSRELAENHSTSESAHNVSMPVESLQPTTFNEIDPVDCDGIYLSS 1440
            TEDSKSVLHVKYIAALSRELAENHSTSESAHNVSMPVESLQPTTFNEIDPVDCDGIYLSS
Sbjct: 1381 TEDSKSVLHVKYIAALSRELAENHSTSESAHNVSMPVESLQPTTFNEIDPVDCDGIYLSS 1440

Query: 1441 CGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAFPREF 1500
            CGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAFPREF
Sbjct: 1441 CGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAFPREF 1500

Query: 1501 QKFGNPSMSSIGSLSHVSGQSNKSTEEVNPLYVQKAVAFLQSTAKAVGKNKVLKDISVHR 1560
            QKFGNPSMSSIGSLSHVSGQSNKSTEEVNPLYVQKAVAFLQSTAKAVGKNKVLKDISVHR
Sbjct: 1501 QKFGNPSMSSIGSLSHVSGQSNKSTEEVNPLYVQKAVAFLQSTAKAVGKNKVLKDISVHR 1560

Query: 1561 HRKVSRNLEAVSLVLSKLYFSGKQDKSISCTRVNPSILMWDTLKYSLVSMEIAARSKTDV 1620
            HRKVSRNLEAVSLVLSKLYFSGKQDKSISCTRVNPSILMWDTLKYSLVSMEIAARSKTDV
Sbjct: 1561 HRKVSRNLEAVSLVLSKLYFSGKQDKSISCTRVNPSILMWDTLKYSLVSMEIAARSKTDV 1620

Query: 1621 NPNIGLNTLYKELKTSGGFILSLLLKVIQSIKCEDSLLLLQRFCGIQRFADSICSGVSNE 1680
            NPNIGLNTLYKELKTSGGFILSLLLKVIQSIKCEDSLLLLQRFCGIQRFADSICSGVSNE
Sbjct: 1621 NPNIGLNTLYKELKTSGGFILSLLLKVIQSIKCEDSLLLLQRFCGIQRFADSICSGVSNE 1680

Query: 1681 NANDSCGQGILHILTSLRVELPQFDIQFLSRGSDPVLAHDPFASVMWVLFCLPCPFLSCK 1740
            NANDSCGQGILHILTSLRVELPQFDIQFLSRGSDPVLAHDPFASVMWVLFCLPCPFLSCK
Sbjct: 1681 NANDSCGQGILHILTSLRVELPQFDIQFLSRGSDPVLAHDPFASVMWVLFCLPCPFLSCK 1740

Query: 1741 ESLISLVHIFYLVSVAQGIIASCMKYQSKIDGMGFSDCLIADICKIMGESEYARRYFVSD 1800
            ESLISLVHIFYLVSVAQGIIASCMKYQSKIDGMGFSDCLIADICKIMGESEYARRYFVSD
Sbjct: 1741 ESLISLVHIFYLVSVAQGIIASCMKYQSKIDGMGFSDCLIADICKIMGESEYARRYFVSD 1800

Query: 1801 YTEPSCNVKDMIRSLTFTYLRRCALLLKLLNSSARAPIFDGETALERYLAVDDMIDNIAL 1860
            YTEPSCNVKDMIRSLTFTYLRRCALLLKLLNSSARAPIFDGETALERYLAVDDMIDNIAL
Sbjct: 1801 YTEPSCNVKDMIRSLTFTYLRRCALLLKLLNSSARAPIFDGETALERYLAVDDMIDNIAL 1860

Query: 1861 ELNEIGKLEKIFKIPPLDVVLKDEASRSLVWKWFCHFNREFEFQRSKNLKHCTPAVPFQL 1920
            ELNEIGKLEKIFKIPPLDVVLKDEASRSLVWKWFCHFNREFEFQRSKNLKHCTPAVPFQL
Sbjct: 1861 ELNEIGKLEKIFKIPPLDVVLKDEASRSLVWKWFCHFNREFEFQRSKNLKHCTPAVPFQL 1920

Query: 1921 IRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANICA 1980
            IRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANICA
Sbjct: 1921 IRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANICA 1980

Query: 1981 AGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYMV 2040
            AGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYMV
Sbjct: 1981 AGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYMV 2037

BLAST of MS019737 vs. ExPASy TrEMBL
Match: A0A1S3B5X9 (E3 ubiquitin-protein ligase OS=Cucumis melo OX=3656 GN=LOC103486385 PE=3 SV=1)

HSP 1 Score: 3540.0 bits (9178), Expect = 0.0e+00
Identity = 1777/2041 (87.07%), Postives = 1888/2041 (92.50%), Query Frame = 0

Query: 1    MDDMDIGSPSESAPLKPRDRIVRRLALLGVPEELLDQLQPGLVSFVKDNKILIPQLVSAI 60
            MDDMDIGSPSES PLKPRDRI+RRLA+LGVP+ELLDQL  GLV FVKDNK LIPQLVSAI
Sbjct: 1    MDDMDIGSPSESTPLKPRDRILRRLAVLGVPDELLDQLFRGLVIFVKDNKFLIPQLVSAI 60

Query: 61   LPTDVEVAEVIRDAKAGSKKSMAGPTMKNQFRESMVWLQWLMFEGEPTFALKNLSKMSFG 120
            LPTDVEV EVIRDA  GSKK++ GPTMK  FRESM+WLQWLMFEGEP +ALKNLSKMS G
Sbjct: 61   LPTDVEVVEVIRDAIPGSKKTLTGPTMKAHFRESMMWLQWLMFEGEPAYALKNLSKMSVG 120

Query: 121  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVT 180
            QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNH+DHDYSIIYTGGGCCDCGD T
Sbjct: 121  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHQDHDYSIIYTGGGCCDCGDAT 180

Query: 181  AWKREGFCSKHKGAERIQPLPEEFVESVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 240
            AWKREGFCSKHKGAE+IQPLPEE+V+SVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV
Sbjct: 181  AWKREGFCSKHKGAEQIQPLPEEYVKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 240

Query: 241  MEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISLAGFLDILVRLERLLTDIVVKK 300
             EHKKVANELTFAVVEMLLDFCK+SESLLSFVSKRVIS AG LDILVRLERLLTD VVKK
Sbjct: 241  TEHKKVANELTFAVVEMLLDFCKHSESLLSFVSKRVISSAGLLDILVRLERLLTDSVVKK 300

Query: 301  VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHALKKYPLLPTFSVQIFTVPT 360
            VHELLLKLLGEP+FKYEFAKVFLNYYPTVISEAIE+ SDHALKKYPLLPTFSVQIFTVPT
Sbjct: 301  VHELLLKLLGEPVFKYEFAKVFLNYYPTVISEAIEDSSDHALKKYPLLPTFSVQIFTVPT 360

Query: 361  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAV 420
            LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQV+KWSNLYETTIRVVED+RFVMSHAV
Sbjct: 361  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVVKWSNLYETTIRVVEDVRFVMSHAV 420

Query: 421  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHTL 480
            VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIH+L
Sbjct: 421  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL 480

Query: 481  LVNEAFSAASSSSSSEETADAMSFHAFKQNPDDMDGIRHAKVGRLSQESAACNVFGRSST 540
            LV EAFSAA  SSS E+TAD M F  +KQN DD+D +RHAKVGRLSQESAAC V G+SST
Sbjct: 481  LVKEAFSAA--SSSCEDTADGMYFQTYKQNVDDIDSVRHAKVGRLSQESAACGVLGKSST 540

Query: 541  VTSASRVDEVCSDTISSTIMWLTYECLKVIDSWLGTENVLGGFPNMFAENASMASSCKVY 600
             TSASRVDEVCSD ISSTIMWLTYECLK+ID+WLGTEN+ G  PNM  E+ S A SCK Y
Sbjct: 541  STSASRVDEVCSDAISSTIMWLTYECLKIIDNWLGTENISGSIPNMLGESISPAPSCKFY 600

Query: 601  SLRKPSALESRKISSKVEKGKFIFEKVAGKSKDHNRQYSSRIYSGLQMSIDNEHGVSLGE 660
            S RK SAL S+K+S K+EKGKFIF+K++ +SK HNRQYSSR+YSGLQMSIDNEHG+SLGE
Sbjct: 601  SSRKTSALASKKMSYKMEKGKFIFDKLSRRSKYHNRQYSSRMYSGLQMSIDNEHGISLGE 660

Query: 661  DNQLMDVTNDTVTVEDYSMETDALHFLSFSAWPNIVYDVSSQDISIHIPLHRLLSLLLQK 720
            DN LMDVTNDTVT EDY+ME DALHFLS S+WPNIVYDVSSQDISIHIPLHRLLSLLLQK
Sbjct: 661  DNHLMDVTNDTVTDEDYAMEIDALHFLSLSSWPNIVYDVSSQDISIHIPLHRLLSLLLQK 720

Query: 721  ALRSSFSESVVPSATGASSSNLSFEYVDFFKSVLTDYHPYGFSAFVMEHPLRIKVFCAEV 780
            AL S FSES VPSATGASSSNLS EYVDFFKSVLTD HPYGFS+FVMEHPLRIKVFCAEV
Sbjct: 721  ALSSCFSESGVPSATGASSSNLSSEYVDFFKSVLTDCHPYGFSSFVMEHPLRIKVFCAEV 780

Query: 781  NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPELYISRILERFRLLD 840
            NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPP+LY+ RILERFRL D
Sbjct: 781  NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVCRILERFRLSD 840

Query: 841  YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQL 900
            YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLT+AESLKRELIYKLAIGDATHSQL
Sbjct: 841  YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTIAESLKRELIYKLAIGDATHSQL 900

Query: 901  VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLRWKYWKELDLYHPRWSLRDLQIA 960
            VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSL WKYWKELDLYHPRWSLRDLQ+A
Sbjct: 901  VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPRWSLRDLQVA 960

Query: 961  EERYLRSCGASALTSQLPKWTKIYPPFRGLARIATCKAVLQIIRAVLFYSVFTDKSTKSR 1020
            EERYLRSCG SALTSQLPKWTKIYPPFRGLARIATCK VLQ IRAVLFYSVF + STKSR
Sbjct: 961  EERYLRSCGVSALTSQLPKWTKIYPPFRGLARIATCKTVLQFIRAVLFYSVFAEISTKSR 1020

Query: 1021 APDSVLLTALHLLALALDICFQRKESSDQSFCSEDSIPLLLFADEEVDEGLTYGLGRQSL 1080
            APDSVLL+ALHLLALALD+CFQ+KES DQSF + DSIPLLLFA EE++EGL YG GRQSL
Sbjct: 1021 APDSVLLSALHLLALALDVCFQQKESRDQSFDAPDSIPLLLFAAEEIEEGLAYGFGRQSL 1080

Query: 1081 LSLLILLMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDSRCMGKIRQHAPEILGYL 1140
            LSLLILLMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDS CMGK++Q APEILGYL
Sbjct: 1081 LSLLILLMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDSHCMGKVQQLAPEILGYL 1140

Query: 1141 SQSLPSSDPNSLSETSDSDKLKAKARERQAAILEKMRAEQYKFLASVDSSV-EDDAEFGQ 1200
            SQS+P+S  +  +ETSDS+K KAKARERQAAILEKMRAEQ KFLASVD+SV +DD EFGQ
Sbjct: 1141 SQSVPTSTTSRPAETSDSEKRKAKARERQAAILEKMRAEQSKFLASVDASVDDDDTEFGQ 1200

Query: 1201 ESEKPSVSDSAEQSETVCSLCHDSSSSVPISYLILLQKSKLVSLIDRGTVSWDQPYWRDE 1260
            E EKP+VSD AEQSETVCSLCHDSSSSVPIS+LILLQKSKLVSLIDRG VSWDQPYWRDE
Sbjct: 1201 EPEKPNVSDDAEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGPVSWDQPYWRDE 1260

Query: 1261 HASSIPRRNLDQSGLSTSSGGSGGISSPQLAELIQNAVKEYASHGLPGEVGAFLDFVKSH 1320
            HAS+  +R++DQSG+STSS GS  ISSPQLAELIQNAVKEY +HGLPGEVGAFLDF+KSH
Sbjct: 1261 HASTTSKRDIDQSGVSTSSAGSVVISSPQLAELIQNAVKEYTNHGLPGEVGAFLDFLKSH 1320

Query: 1321 FPPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHENMHSKLNEDENFSKVASSL 1380
            FPPLR+IQVPGTSNVKGEKIIFSFDTLEEDIYLSV KEMH+ +HSK +E E  S VAS  
Sbjct: 1321 FPPLRDIQVPGTSNVKGEKIIFSFDTLEEDIYLSVFKEMHDTLHSKFHEGEKISTVASG- 1380

Query: 1381 VTEDSKSVLHVKYIAALSRELAENHSTSESAHNVSMPVESLQPTTFNEIDPVDCDGIYLS 1440
              EDS+SVLHVKYIAALSRELAEN STSESA NV MPVES QPT  NEI P+DCDGIYLS
Sbjct: 1381 --EDSRSVLHVKYIAALSRELAENRSTSESARNVHMPVESSQPTILNEIGPIDCDGIYLS 1440

Query: 1441 SCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAFPRE 1500
            SCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRL+NSTLPAFPRE
Sbjct: 1441 SCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLSNSTLPAFPRE 1500

Query: 1501 FQKFGNPSMSSIGSLSHVSGQSNKSTEEVNPLYVQKAVAFLQSTAKAVGKNKVLKDISVH 1560
            FQK  NP  SS+G+LSHVSG  NKS E VNPLY+Q+AVA LQS AKAVGKN VLKDISVH
Sbjct: 1501 FQKIWNPRTSSVGTLSHVSGHLNKSNERVNPLYIQEAVALLQSAAKAVGKNNVLKDISVH 1560

Query: 1561 RHRKVSRNLEAVSLVLSKLYFSGKQDKSISCTRVNPSILMWDTLKYSLVSMEIAARSKTD 1620
            RH+KVSRNLEAVSLVLSKLYFS KQDK IS +RVNPSILMWDTLKYSLVSMEIAARSKTD
Sbjct: 1561 RHKKVSRNLEAVSLVLSKLYFSWKQDKLISSSRVNPSILMWDTLKYSLVSMEIAARSKTD 1620

Query: 1621 VNPNIGLNTLYKELKTSGGFILSLLLKVIQSIKCEDSLLLLQRFCGIQRFADSICSGVSN 1680
            +NP+IGLNTLYKELKTSGGF+LSLLLKVI S+KCEDSLLLLQR CGIQRFADSICSG+SN
Sbjct: 1621 MNPSIGLNTLYKELKTSGGFVLSLLLKVIHSVKCEDSLLLLQRLCGIQRFADSICSGMSN 1680

Query: 1681 ENANDSCGQGILHILTSLRVELPQFDIQFLSRGSDPVLAHDPFASVMWVLFCLPCPFLSC 1740
            ENA+DSCG+GILHILTSLR ELP FD QFLSRGSDPV+AHDPFAS+MWVLFCLP PFLSC
Sbjct: 1681 ENASDSCGRGILHILTSLRSELPHFDSQFLSRGSDPVIAHDPFASLMWVLFCLPFPFLSC 1740

Query: 1741 KESLISLVHIFYLVSVAQGIIASCMKYQSKIDGMGFSDCLIADICKIMGESEYARRYFVS 1800
            +ESL+SLVHIFYLVSV Q II    K Q K+DG+G SDCLI DICKIMGESEYAR+YFVS
Sbjct: 1741 RESLLSLVHIFYLVSVTQAIITYFTKSQCKVDGLGSSDCLITDICKIMGESEYARQYFVS 1800

Query: 1801 DYTEPSCNVKDMIRSLTFTYLRRCALLLKLLNSSARAPIFDGETALERYLAVDDMIDNIA 1860
            +YTEPSCN+KD +RSLTF +LRRCALLL+LL+SSAR PIFDGETALE YL  ++MIDNI 
Sbjct: 1801 NYTEPSCNIKDTVRSLTFPFLRRCALLLQLLSSSARVPIFDGETALETYLVGNNMIDNIT 1860

Query: 1861 LELNEIGKLEKIFKIPPLDVVLKDEASRSLVWKWFCHFNREFEFQRSKNLKHCTPAVPFQ 1920
            +ELNE+ KL+K+F IPPLD+VLKD  SR LV KWFCHFN+EFEFQRSK +KHCTPAVPFQ
Sbjct: 1861 VELNEVEKLQKMFDIPPLDIVLKDGTSRLLVSKWFCHFNKEFEFQRSKIIKHCTPAVPFQ 1920

Query: 1921 LIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANIC 1980
            LIRLPHVYHDLLQRYIKKRC DCKHVIDDPALCL+CG+LCSPSWKSCCRESGCQAHANIC
Sbjct: 1921 LIRLPHVYHDLLQRYIKKRCTDCKHVIDDPALCLICGKLCSPSWKSCCRESGCQAHANIC 1980

Query: 1981 AAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYM 2040
            AAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYM
Sbjct: 1981 AAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYM 2036

BLAST of MS019737 vs. ExPASy TrEMBL
Match: A0A5A7TL84 (E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G004420 PE=3 SV=1)

HSP 1 Score: 3540.0 bits (9178), Expect = 0.0e+00
Identity = 1777/2041 (87.07%), Postives = 1888/2041 (92.50%), Query Frame = 0

Query: 1    MDDMDIGSPSESAPLKPRDRIVRRLALLGVPEELLDQLQPGLVSFVKDNKILIPQLVSAI 60
            MDDMDIGSPSES PLKPRDRI+RRLA+LGVP+ELLDQL  GLV FVKDNK LIPQLVSAI
Sbjct: 1    MDDMDIGSPSESTPLKPRDRILRRLAVLGVPDELLDQLFRGLVIFVKDNKFLIPQLVSAI 60

Query: 61   LPTDVEVAEVIRDAKAGSKKSMAGPTMKNQFRESMVWLQWLMFEGEPTFALKNLSKMSFG 120
            LPTDVEV EVIRDA  GSKK++ GPTMK  FRESM+WLQWLMFEGEP +ALKNLSKMS G
Sbjct: 61   LPTDVEVVEVIRDAIPGSKKTLTGPTMKAHFRESMMWLQWLMFEGEPAYALKNLSKMSVG 120

Query: 121  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVT 180
            QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNH+DHDYSIIYTGGGCCDCGD T
Sbjct: 121  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHQDHDYSIIYTGGGCCDCGDAT 180

Query: 181  AWKREGFCSKHKGAERIQPLPEEFVESVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 240
            AWKREGFCSKHKGAE+IQPLPEE+V+SVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV
Sbjct: 181  AWKREGFCSKHKGAEQIQPLPEEYVKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 240

Query: 241  MEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISLAGFLDILVRLERLLTDIVVKK 300
             EHKKVANELTFAVVEMLLDFCK+SESLLSFVSKRVIS AG LDILVRLERLLTD VVKK
Sbjct: 241  TEHKKVANELTFAVVEMLLDFCKHSESLLSFVSKRVISSAGLLDILVRLERLLTDSVVKK 300

Query: 301  VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHALKKYPLLPTFSVQIFTVPT 360
            VHELLLKLLGEP+FKYEFAKVFLNYYPTVISEAIE+ SDHALKKYPLLPTFSVQIFTVPT
Sbjct: 301  VHELLLKLLGEPVFKYEFAKVFLNYYPTVISEAIEDSSDHALKKYPLLPTFSVQIFTVPT 360

Query: 361  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAV 420
            LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQV+KWSNLYETTIRVVED+RFVMSHAV
Sbjct: 361  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVVKWSNLYETTIRVVEDVRFVMSHAV 420

Query: 421  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHTL 480
            VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIH+L
Sbjct: 421  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL 480

Query: 481  LVNEAFSAASSSSSSEETADAMSFHAFKQNPDDMDGIRHAKVGRLSQESAACNVFGRSST 540
            LV EAFSAA  SSS E+TAD M F  +KQN DD+D +RHAKVGRLSQESAAC V G+SST
Sbjct: 481  LVKEAFSAA--SSSCEDTADGMYFQTYKQNVDDIDSVRHAKVGRLSQESAACGVLGKSST 540

Query: 541  VTSASRVDEVCSDTISSTIMWLTYECLKVIDSWLGTENVLGGFPNMFAENASMASSCKVY 600
             TSASRVDEVCSD ISSTIMWLTYECLK+ID+WLGTEN+ G  PNM  E+ S A SCK Y
Sbjct: 541  STSASRVDEVCSDAISSTIMWLTYECLKIIDNWLGTENISGSIPNMLGESISPAPSCKFY 600

Query: 601  SLRKPSALESRKISSKVEKGKFIFEKVAGKSKDHNRQYSSRIYSGLQMSIDNEHGVSLGE 660
            S RK SAL S+K+S K+EKGKFIF+K++ +SK HNRQYSSR+YSGLQMSIDNEHG+SLGE
Sbjct: 601  SSRKTSALASKKMSYKMEKGKFIFDKLSRRSKYHNRQYSSRMYSGLQMSIDNEHGISLGE 660

Query: 661  DNQLMDVTNDTVTVEDYSMETDALHFLSFSAWPNIVYDVSSQDISIHIPLHRLLSLLLQK 720
            DN LMDVTNDTVT EDY+ME DALHFLS S+WPNIVYDVSSQDISIHIPLHRLLSLLLQK
Sbjct: 661  DNHLMDVTNDTVTDEDYAMEIDALHFLSLSSWPNIVYDVSSQDISIHIPLHRLLSLLLQK 720

Query: 721  ALRSSFSESVVPSATGASSSNLSFEYVDFFKSVLTDYHPYGFSAFVMEHPLRIKVFCAEV 780
            AL S FSES VPSATGASSSNLS EYVDFFKSVLTD HPYGFS+FVMEHPLRIKVFCAEV
Sbjct: 721  ALSSCFSESGVPSATGASSSNLSSEYVDFFKSVLTDCHPYGFSSFVMEHPLRIKVFCAEV 780

Query: 781  NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPELYISRILERFRLLD 840
            NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPP+LY+ RILERFRL D
Sbjct: 781  NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVCRILERFRLSD 840

Query: 841  YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQL 900
            YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLT+AESLKRELIYKLAIGDATHSQL
Sbjct: 841  YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTIAESLKRELIYKLAIGDATHSQL 900

Query: 901  VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLRWKYWKELDLYHPRWSLRDLQIA 960
            VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSL WKYWKELDLYHPRWSLRDLQ+A
Sbjct: 901  VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPRWSLRDLQVA 960

Query: 961  EERYLRSCGASALTSQLPKWTKIYPPFRGLARIATCKAVLQIIRAVLFYSVFTDKSTKSR 1020
            EERYLRSCG SALTSQLPKWTKIYPPFRGLARIATCK VLQ IRAVLFYSVF + STKSR
Sbjct: 961  EERYLRSCGVSALTSQLPKWTKIYPPFRGLARIATCKTVLQFIRAVLFYSVFAEISTKSR 1020

Query: 1021 APDSVLLTALHLLALALDICFQRKESSDQSFCSEDSIPLLLFADEEVDEGLTYGLGRQSL 1080
            APDSVLL+ALHLLALALD+CFQ+KES DQSF + DSIPLLLFA EE++EGL YG GRQSL
Sbjct: 1021 APDSVLLSALHLLALALDVCFQQKESRDQSFDAPDSIPLLLFAAEEIEEGLAYGFGRQSL 1080

Query: 1081 LSLLILLMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDSRCMGKIRQHAPEILGYL 1140
            LSLLILLMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDS CMGK++Q APEILGYL
Sbjct: 1081 LSLLILLMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDSHCMGKVQQLAPEILGYL 1140

Query: 1141 SQSLPSSDPNSLSETSDSDKLKAKARERQAAILEKMRAEQYKFLASVDSSV-EDDAEFGQ 1200
            SQS+P+S  +  +ETSDS+K KAKARERQAAILEKMRAEQ KFLASVD+SV +DD EFGQ
Sbjct: 1141 SQSVPTSTTSRPAETSDSEKRKAKARERQAAILEKMRAEQSKFLASVDASVDDDDTEFGQ 1200

Query: 1201 ESEKPSVSDSAEQSETVCSLCHDSSSSVPISYLILLQKSKLVSLIDRGTVSWDQPYWRDE 1260
            E EKP+VSD AEQSETVCSLCHDSSSSVPIS+LILLQKSKLVSLIDRG VSWDQPYWRDE
Sbjct: 1201 EPEKPNVSDDAEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGPVSWDQPYWRDE 1260

Query: 1261 HASSIPRRNLDQSGLSTSSGGSGGISSPQLAELIQNAVKEYASHGLPGEVGAFLDFVKSH 1320
            HAS+  +R++DQSG+STSS GS  ISSPQLAELIQNAVKEY +HGLPGEVGAFLDF+KSH
Sbjct: 1261 HASTTSKRDIDQSGVSTSSAGSVVISSPQLAELIQNAVKEYTNHGLPGEVGAFLDFLKSH 1320

Query: 1321 FPPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHENMHSKLNEDENFSKVASSL 1380
            FPPLR+IQVPGTSNVKGEKIIFSFDTLEEDIYLSV KEMH+ +HSK +E E  S VAS  
Sbjct: 1321 FPPLRDIQVPGTSNVKGEKIIFSFDTLEEDIYLSVFKEMHDTLHSKFHEGEKISTVASG- 1380

Query: 1381 VTEDSKSVLHVKYIAALSRELAENHSTSESAHNVSMPVESLQPTTFNEIDPVDCDGIYLS 1440
              EDS+SVLHVKYIAALSRELAEN STSESA NV MPVES QPT  NEI P+DCDGIYLS
Sbjct: 1381 --EDSRSVLHVKYIAALSRELAENRSTSESARNVHMPVESSQPTILNEIGPIDCDGIYLS 1440

Query: 1441 SCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAFPRE 1500
            SCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRL+NSTLPAFPRE
Sbjct: 1441 SCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLSNSTLPAFPRE 1500

Query: 1501 FQKFGNPSMSSIGSLSHVSGQSNKSTEEVNPLYVQKAVAFLQSTAKAVGKNKVLKDISVH 1560
            FQK  NP  SS+G+LSHVSG  NKS E VNPLY+Q+AVA LQS AKAVGKN VLKDISVH
Sbjct: 1501 FQKIWNPRTSSVGTLSHVSGHLNKSNERVNPLYIQEAVALLQSAAKAVGKNNVLKDISVH 1560

Query: 1561 RHRKVSRNLEAVSLVLSKLYFSGKQDKSISCTRVNPSILMWDTLKYSLVSMEIAARSKTD 1620
            RH+KVSRNLEAVSLVLSKLYFS KQDK IS +RVNPSILMWDTLKYSLVSMEIAARSKTD
Sbjct: 1561 RHKKVSRNLEAVSLVLSKLYFSWKQDKLISSSRVNPSILMWDTLKYSLVSMEIAARSKTD 1620

Query: 1621 VNPNIGLNTLYKELKTSGGFILSLLLKVIQSIKCEDSLLLLQRFCGIQRFADSICSGVSN 1680
            +NP+IGLNTLYKELKTSGGF+LSLLLKVI S+KCEDSLLLLQR CGIQRFADSICSG+SN
Sbjct: 1621 MNPSIGLNTLYKELKTSGGFVLSLLLKVIHSVKCEDSLLLLQRLCGIQRFADSICSGMSN 1680

Query: 1681 ENANDSCGQGILHILTSLRVELPQFDIQFLSRGSDPVLAHDPFASVMWVLFCLPCPFLSC 1740
            ENA+DSCG+GILHILTSLR ELP FD QFLSRGSDPV+AHDPFAS+MWVLFCLP PFLSC
Sbjct: 1681 ENASDSCGRGILHILTSLRSELPHFDSQFLSRGSDPVIAHDPFASLMWVLFCLPFPFLSC 1740

Query: 1741 KESLISLVHIFYLVSVAQGIIASCMKYQSKIDGMGFSDCLIADICKIMGESEYARRYFVS 1800
            +ESL+SLVHIFYLVSV Q II    K Q K+DG+G SDCLI DICKIMGESEYAR+YFVS
Sbjct: 1741 RESLLSLVHIFYLVSVTQAIITYFTKSQCKVDGLGSSDCLITDICKIMGESEYARQYFVS 1800

Query: 1801 DYTEPSCNVKDMIRSLTFTYLRRCALLLKLLNSSARAPIFDGETALERYLAVDDMIDNIA 1860
            +YTEPSCN+KD +RSLTF +LRRCALLL+LL+SSAR PIFDGETALE YL  ++MIDNI 
Sbjct: 1801 NYTEPSCNIKDTVRSLTFPFLRRCALLLQLLSSSARVPIFDGETALETYLVGNNMIDNIT 1860

Query: 1861 LELNEIGKLEKIFKIPPLDVVLKDEASRSLVWKWFCHFNREFEFQRSKNLKHCTPAVPFQ 1920
            +ELNE+ KL+K+F IPPLD+VLKD  SR LV KWFCHFN+EFEFQRSK +KHCTPAVPFQ
Sbjct: 1861 VELNEVEKLQKMFDIPPLDIVLKDGTSRLLVSKWFCHFNKEFEFQRSKIIKHCTPAVPFQ 1920

Query: 1921 LIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANIC 1980
            LIRLPHVYHDLLQRYIKKRC DCKHVIDDPALCL+CG+LCSPSWKSCCRESGCQAHANIC
Sbjct: 1921 LIRLPHVYHDLLQRYIKKRCTDCKHVIDDPALCLICGKLCSPSWKSCCRESGCQAHANIC 1980

Query: 1981 AAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYM 2040
            AAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYM
Sbjct: 1981 AAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYM 2036

BLAST of MS019737 vs. ExPASy TrEMBL
Match: A0A6J1J0N1 (E3 ubiquitin-protein ligase OS=Cucurbita maxima OX=3661 GN=LOC111482399 PE=3 SV=1)

HSP 1 Score: 3531.5 bits (9156), Expect = 0.0e+00
Identity = 1781/2041 (87.26%), Postives = 1881/2041 (92.16%), Query Frame = 0

Query: 1    MDDMDIGSPSESAPLKPRDRIVRRLALLGVPEELLDQLQPGLVSFVKDNKILIPQLVSAI 60
            MDDMDIGSPSESAPLKPRDRI+RRLA+ GVP+ELLDQL  GLVSFVKDNK LIPQLVSAI
Sbjct: 1    MDDMDIGSPSESAPLKPRDRILRRLAVFGVPDELLDQLFCGLVSFVKDNKFLIPQLVSAI 60

Query: 61   LPTDVEVAEVIRDAKAGSKKSMAGPTMKNQFRESMVWLQWLMFEGEPTFALKNLSKMSFG 120
            LPTDVEV E IRDAKAGSKKS+ GPTMK QFRESM+WLQWLMFEGEP +ALKNLSKMS G
Sbjct: 61   LPTDVEVVEFIRDAKAGSKKSLTGPTMKTQFRESMMWLQWLMFEGEPAYALKNLSKMSIG 120

Query: 121  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVT 180
            QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNH+DHDYSIIYTGGGCCDCGD T
Sbjct: 121  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHQDHDYSIIYTGGGCCDCGDAT 180

Query: 181  AWKREGFCSKHKGAERIQPLPEEFVESVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 240
            AWKREGFCSKHKGAE+IQPLPEEFV+SVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV
Sbjct: 181  AWKREGFCSKHKGAEQIQPLPEEFVKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 240

Query: 241  MEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISLAGFLDILVRLERLLTDIVVKK 300
             EHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVIS AG LDILVRLERLLTD VVKK
Sbjct: 241  TEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGLLDILVRLERLLTDSVVKK 300

Query: 301  VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHALKKYPLLPTFSVQIFTVPT 360
            VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIE+ SDHALKKYPLLPTFSVQIFTVPT
Sbjct: 301  VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEDSSDHALKKYPLLPTFSVQIFTVPT 360

Query: 361  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAV 420
            LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVED+RFVMSHAV
Sbjct: 361  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDVRFVMSHAV 420

Query: 421  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHTL 480
            VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIH+L
Sbjct: 421  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL 480

Query: 481  LVNEAFSAASSSSSSEETADAMSFHAFKQNPDDMDGIRHAKVGRLSQESAACNVFGRSST 540
            LV EAFS A + SS E+TADAMSF  +KQN DD+D +RHAKVGRLSQE+AACNV G+ S 
Sbjct: 481  LVKEAFSPA-NCSSREDTADAMSFQTYKQNVDDIDNVRHAKVGRLSQETAACNVLGKGSR 540

Query: 541  VTSASRVDEVCSDTISSTIMWLTYECLKVIDSWLGTENVLGGFPNMFAENASMASSCKVY 600
             TSASRVDEVC D IS TIMWLTYECLK+IDSWL TE+  G  PNM  ++ S++ SCK Y
Sbjct: 541  STSASRVDEVCLDKISPTIMWLTYECLKIIDSWLATESTYGSIPNMLDDSISLSPSCKSY 600

Query: 601  SLRKPSALESRKISSKVEKGKFIFEKVAGKSKDHNRQYSSRIYSGLQMSIDNEHGVSLGE 660
            SLRK SA       SK+EKGKFIFEK+A +SK H RQYSSR+YSGLQMSIDNEHG+S GE
Sbjct: 601  SLRKTSA------PSKMEKGKFIFEKLAKRSKYHIRQYSSRMYSGLQMSIDNEHGISCGE 660

Query: 661  DNQLMDVTNDTVTVEDYSMETDALHFLSFSAWPNIVYDVSSQDISIHIPLHRLLSLLLQK 720
            D+QLMDVTNDTVT EDY+ME DALHFLS SAWPNIVYDVSSQDISIH+PLHRLLSLLLQK
Sbjct: 661  DSQLMDVTNDTVTDEDYAMEVDALHFLSSSAWPNIVYDVSSQDISIHVPLHRLLSLLLQK 720

Query: 721  ALRSSFSESVVPSATGASSSNLSFEYVDFFKSVLTDYHPYGFSAFVMEHPLRIKVFCAEV 780
            AL S FSESVVPSATGASSSNLS EYVDFFKSVLTD HPYGFS+FVMEHPLRIKVFCAEV
Sbjct: 721  ALGSCFSESVVPSATGASSSNLSSEYVDFFKSVLTDCHPYGFSSFVMEHPLRIKVFCAEV 780

Query: 781  NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPELYISRILERFRLLD 840
            NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPP+LY+SRI+ERFRL D
Sbjct: 781  NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVSRIIERFRLSD 840

Query: 841  YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQL 900
            Y+SLNVERPSEYEP LVQEMLTLIIQ+VNERRFCGLTVAESLKRELIYKLAIGDATHSQL
Sbjct: 841  YISLNVERPSEYEPTLVQEMLTLIIQIVNERRFCGLTVAESLKRELIYKLAIGDATHSQL 900

Query: 901  VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLRWKYWKELDLYHPRWSLRDLQIA 960
            VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSL WKYWKELDLYHPRWSLRDLQ+A
Sbjct: 901  VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPRWSLRDLQVA 960

Query: 961  EERYLRSCGASALTSQLPKWTKIYPPFRGLARIATCKAVLQIIRAVLFYSVFTDKSTKSR 1020
            EERYLRSC  SALTSQLPKWTK YPPF+GLARIATCK VLQ +RAVLFYSVFT+KSTKSR
Sbjct: 961  EERYLRSCSVSALTSQLPKWTKTYPPFKGLARIATCKTVLQFVRAVLFYSVFTEKSTKSR 1020

Query: 1021 APDSVLLTALHLLALALDICFQRKESSDQSFCSEDSIPLLLFADEEVDEGLTYGLGRQSL 1080
            APDSVLL+ALHLLALALDICFQ+KESSDQSF   DSIPLLLFA EE+DEGL YG GRQSL
Sbjct: 1021 APDSVLLSALHLLALALDICFQQKESSDQSFYGPDSIPLLLFAAEEIDEGLAYGFGRQSL 1080

Query: 1081 LSLLILLMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDSRCMGKIRQHAPEILGYL 1140
            LSLLILLMKMHKKEGREN LEAGSCNLSSLVESLLKKFSE+DS CMGK++Q APEILGYL
Sbjct: 1081 LSLLILLMKMHKKEGRENFLEAGSCNLSSLVESLLKKFSEVDSHCMGKVQQLAPEILGYL 1140

Query: 1141 SQSLPSSDPNSLSETSDSDKLKAKARERQAAILEKMRAEQYKFLASVDSSV-EDDAEFGQ 1200
            S+SLPSS+ N  S +SDS+K KAKARERQAAILEKMRAEQ KFLASVD+SV +DD EFGQ
Sbjct: 1141 SESLPSSNTNRPSRSSDSEKRKAKARERQAAILEKMRAEQSKFLASVDASVDDDDPEFGQ 1200

Query: 1201 ESEKPSVSDSAEQSETVCSLCHDSSSSVPISYLILLQKSKLVSLIDRGTVSWDQPYWRDE 1260
            ESEKP VS SAEQSETVCSLCHDSSSSVPIS+L LLQKSKLVSLIDRG VSWDQPYWRDE
Sbjct: 1201 ESEKPDVSVSAEQSETVCSLCHDSSSSVPISFLTLLQKSKLVSLIDRGVVSWDQPYWRDE 1260

Query: 1261 HASSIPRRNLDQSGLSTSSGGSGGISSPQLAELIQNAVKEYASHGLPGEVGAFLDFVKSH 1320
            HAS++ +RNLDQSGLSTSS  SGGISS QL ELIQNAVKEYA+HGLPGEVGAFLDFVKSH
Sbjct: 1261 HASTMSKRNLDQSGLSTSSASSGGISSLQLTELIQNAVKEYANHGLPGEVGAFLDFVKSH 1320

Query: 1321 FPPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHENMHSKLNEDENFSKVASSL 1380
            FPPLRN QVPGTSN KGEKIIFSFDTLEEDIYLSV+KEMH+++HSK  EDENFSK     
Sbjct: 1321 FPPLRNSQVPGTSNDKGEKIIFSFDTLEEDIYLSVQKEMHDSLHSKFYEDENFSKAVRG- 1380

Query: 1381 VTEDSKSVLHVKYIAALSRELAENHSTSESAHNVSMPVESLQPTTFNEIDPVDCDGIYLS 1440
              EDS SVLHVKYIAALSRELAENH+TS SAHNV   VE LQPT FNEI P DCDGIYLS
Sbjct: 1381 --EDSMSVLHVKYIAALSRELAENHATSVSAHNVDTLVEHLQPTAFNEIGPTDCDGIYLS 1440

Query: 1441 SCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAFPRE 1500
            SCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLAN TLPA+PRE
Sbjct: 1441 SCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANCTLPAYPRE 1500

Query: 1501 FQKFGNPSMSSIGSLSHVSGQSNKSTEEVNPLYVQKAVAFLQSTAKAVGKNKVLKDISVH 1560
             Q   NP+ SS+GSLS VSG  NKSTE  NPLY+Q+AVA LQS AKAVG+NKVLKDISVH
Sbjct: 1501 SQNIWNPATSSVGSLSRVSGHLNKSTERGNPLYIQQAVALLQSAAKAVGRNKVLKDISVH 1560

Query: 1561 RHRKVSRNLEAVSLVLSKLYFSGKQDKSISCTRVNPSILMWDTLKYSLVSMEIAARSKTD 1620
             HRKVS +LE VSLVLSK+YFS KQDK IS +R+NPSILMWD LKYSL+SMEIAARSKTD
Sbjct: 1561 GHRKVSHDLEVVSLVLSKMYFSEKQDKLISSSRINPSILMWDMLKYSLISMEIAARSKTD 1620

Query: 1621 VNPNIGLNTLYKELKTSGGFILSLLLKVIQSIKCEDSLLLLQRFCGIQRFADSICSGVSN 1680
            +NPNIGLNTLYKELKTSGGF+LSLLLKVIQ +KCEDSLLLLQRFCGIQRFADSICSG+SN
Sbjct: 1621 MNPNIGLNTLYKELKTSGGFVLSLLLKVIQRVKCEDSLLLLQRFCGIQRFADSICSGMSN 1680

Query: 1681 ENANDSCGQGILHILTSLRVELPQFDIQFLSRGSDPVLAHDPFASVMWVLFCLPCPFLSC 1740
            ENA+DSCG+GILHILTSLRVELPQFDIQFLSRGSDPVLAHDPFAS+MWVLFCLP PFLSC
Sbjct: 1681 ENASDSCGRGILHILTSLRVELPQFDIQFLSRGSDPVLAHDPFASLMWVLFCLPFPFLSC 1740

Query: 1741 KESLISLVHIFYLVSVAQGIIASCMKYQSKIDGMGFSDCLIADICKIMGESEYARRYFVS 1800
            +ESL+SLVHIFYLVSVAQ II+S +K Q K+D +GFSD L+ADICKIM ESE+AR+YFVS
Sbjct: 1741 RESLLSLVHIFYLVSVAQAIISSFIKSQYKVDELGFSDSLVADICKIMEESEHARQYFVS 1800

Query: 1801 DYTEPSCNVKDMIRSLTFTYLRRCALLLKLLNSSARAPIFDGETALERYLAVDDMIDNIA 1860
            +YTEPSCNVKDMIRS TF YLRRCALLLKLLNS A  PIFDGETALE YLA ++MIDN  
Sbjct: 1801 NYTEPSCNVKDMIRSWTFPYLRRCALLLKLLNSCALVPIFDGETALETYLAGNNMIDNNT 1860

Query: 1861 LELNEIGKLEKIFKIPPLDVVLKDEASRSLVWKWFCHFNREFEFQRSKNLKHCTPAVPFQ 1920
            LELNE  KLEKIF+I PLD+V KD  SR+LV KWFCHFN+EFEFQRSKN+KHCTPAVPFQ
Sbjct: 1861 LELNENEKLEKIFEILPLDIVFKDGTSRALVSKWFCHFNKEFEFQRSKNIKHCTPAVPFQ 1920

Query: 1921 LIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANIC 1980
            LIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANIC
Sbjct: 1921 LIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANIC 1980

Query: 1981 AAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYM 2040
            AAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYM
Sbjct: 1981 AAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYM 2031

BLAST of MS019737 vs. ExPASy TrEMBL
Match: A0A0A0LD61 (E3 ubiquitin-protein ligase OS=Cucumis sativus OX=3659 GN=Csa_3G734220 PE=3 SV=1)

HSP 1 Score: 3527.3 bits (9145), Expect = 0.0e+00
Identity = 1776/2042 (86.97%), Postives = 1896/2042 (92.85%), Query Frame = 0

Query: 1    MDDMDIGSPSESAPLKPRDRIVRRLALLGVPEELLDQLQPGLVSFVKDNKILIPQLVSAI 60
            MD+MDIGSPSESAPLKPRDRI+RRLA+LGVP+ELLDQL  GLV+FVKDNK LIP+LVSAI
Sbjct: 1    MDEMDIGSPSESAPLKPRDRILRRLAVLGVPDELLDQLFRGLVTFVKDNKFLIPELVSAI 60

Query: 61   LPTDVEVAEVIRDAKAGSKKSMAGPTMKNQFRESMVWLQWLMFEGEPTFALKNLSKMSFG 120
            LPTDVEV EVIRDA  G+KKS+AGPTMK  FRESM+WLQWLMFE EP +ALKNLSKMS G
Sbjct: 61   LPTDVEVVEVIRDAIPGAKKSLAGPTMKANFRESMMWLQWLMFESEPAYALKNLSKMSVG 120

Query: 121  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVT 180
            QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNH+DHDYSIIYTGGGCCDCGD T
Sbjct: 121  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHQDHDYSIIYTGGGCCDCGDAT 180

Query: 181  AWKREGFCSKHKGAERIQPLPEEFVESVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 240
            AWKREGFCSKHKGAE+IQPLPEE+V+SVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV
Sbjct: 181  AWKREGFCSKHKGAEQIQPLPEEYVKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 240

Query: 241  MEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISLAGFLDILVRLERLLTDIVVKK 300
             EHKKVANELTFAVVEMLLDFCK+SESLLSFVSKRVIS AG LDILVRLERLLTD VVKK
Sbjct: 241  TEHKKVANELTFAVVEMLLDFCKHSESLLSFVSKRVISSAGLLDILVRLERLLTDGVVKK 300

Query: 301  VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHALKKYPLLPTFSVQIFTVPT 360
            VHELLLKLLGEP+FKYEFAKVFLNYYPTVISEAIE+ SDHALKKYPLLPTFSVQIFTVPT
Sbjct: 301  VHELLLKLLGEPVFKYEFAKVFLNYYPTVISEAIEDSSDHALKKYPLLPTFSVQIFTVPT 360

Query: 361  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAV 420
            LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQV+KWSNLYETTIRVVED+RFVMSHAV
Sbjct: 361  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVVKWSNLYETTIRVVEDVRFVMSHAV 420

Query: 421  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHTL 480
            VPRYVIY+QQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIH+L
Sbjct: 421  VPRYVIYQQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL 480

Query: 481  LVNEAFSAASSSSSSEETADAMSFHAFKQNPDDMDGIRHAKVGRLSQESAACNVFGRSST 540
            LV EAFSAA SSSS E++ADAM F  +KQN DD+D +RHAKVGRLSQ+SAACNV G+SS 
Sbjct: 481  LVKEAFSAA-SSSSCEDSADAMYFQTYKQNVDDIDSVRHAKVGRLSQDSAACNVLGKSSA 540

Query: 541  VTSASRVDEVCSDTISSTIMWLTYECLKVIDSWLGTENVLGGFPNMFAENASMASSCKVY 600
             TSASRVD+VCSD ISSTIMWLTYECLK+IDSWLGTEN+ G  PNM  E+ S+A SCK Y
Sbjct: 541  STSASRVDDVCSDAISSTIMWLTYECLKIIDSWLGTENISGSIPNMLDESISLAPSCKFY 600

Query: 601  SLRKPSALESRKISSKVEKGKFIFEKVAGKSKDHNRQYSSRIYSGLQMSIDNEHGVSLGE 660
            SLRK SAL S+K+S K+EKGK  FEK++ +SK HNRQYSSR+YSGLQMSIDNEHG+SLGE
Sbjct: 601  SLRKTSALASKKLSYKMEKGK--FEKLSRRSKYHNRQYSSRMYSGLQMSIDNEHGISLGE 660

Query: 661  DNQLMDVTNDTVTVEDYSMETDALHFLSFSAWPNIVYDVSSQDISIHIPLHRLLSLLLQK 720
            DN LMDVTNDTVT EDY+ME DALHFLS S+WPNIVYDVSSQDISIHIPLHRLLSLLLQK
Sbjct: 661  DNHLMDVTNDTVTDEDYAMEIDALHFLSLSSWPNIVYDVSSQDISIHIPLHRLLSLLLQK 720

Query: 721  ALRSSFSESVVPSATGASSSNLSFEYVDFFKSVLTDYHPYGFSAFVMEHPLRIKVFCAEV 780
            ALRS FSES VPSATGASSSNLS EYVDFFKSVLTD HP+GFS+FVMEHPLRIKVFCAEV
Sbjct: 721  ALRSCFSESGVPSATGASSSNLSSEYVDFFKSVLTDCHPFGFSSFVMEHPLRIKVFCAEV 780

Query: 781  NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPELYISRILERFRLLD 840
            NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPP+LY+SRILERFRL +
Sbjct: 781  NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVSRILERFRLSN 840

Query: 841  YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQL 900
            YLSL+VERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQL
Sbjct: 841  YLSLDVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQL 900

Query: 901  VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLRWKYWKELDLYHPRWSLRDLQIA 960
            VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSL WKYWKELDLYHPRWSLRDLQ+A
Sbjct: 901  VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPRWSLRDLQVA 960

Query: 961  EERYLRSCGASALTSQLPKWTKIYPPFRGLARIATCKAVLQIIRAVLFYSVFTDKSTKSR 1020
            EERYLRSCG SALTSQLPKWTKIYPPFRGLARIATCK  LQ IRAVLFYSVF++ STKSR
Sbjct: 961  EERYLRSCGVSALTSQLPKWTKIYPPFRGLARIATCKTALQFIRAVLFYSVFSEISTKSR 1020

Query: 1021 APDSVLLTALHLLALALDICFQRKESSDQSFCSEDSIPLLLFADEEVDEGLTYGLGRQSL 1080
            APDSVLL+ALHLLALALDICFQ+KESSDQSF + DSIPLLLFA EE+DEGL YG GRQSL
Sbjct: 1021 APDSVLLSALHLLALALDICFQQKESSDQSFDAPDSIPLLLFATEEIDEGLAYGFGRQSL 1080

Query: 1081 LSLLILLMKMH-KKEGRENLLEAGSCNLSSLVESLLKKFSEIDSRCMGKIRQHAPEILGY 1140
            LSLLILLMKMH KKEGRENLLEAGSCNLSSLVESLLKKFSEIDS CMGK++Q APEILGY
Sbjct: 1081 LSLLILLMKMHKKKEGRENLLEAGSCNLSSLVESLLKKFSEIDSHCMGKVQQLAPEILGY 1140

Query: 1141 LSQSLPSSDPNSLSETSDSDKLKAKARERQAAILEKMRAEQYKFLASVDSSV-EDDAEFG 1200
            LSQS+P+S  +  +ETSDS+K KAKARERQAAILEKMRAEQ KFLASVD+SV +DD EFG
Sbjct: 1141 LSQSVPTSTTSRPTETSDSEKRKAKARERQAAILEKMRAEQSKFLASVDASVDDDDTEFG 1200

Query: 1201 QESEKPSVSDSAEQSETVCSLCHDSSSSVPISYLILLQKSKLVSLIDRGTVSWDQPYWRD 1260
            QE EKP+VSDSAEQSETVCSLCHDSSSSVPIS+LILLQKSKLVSLIDRG VSWDQPY RD
Sbjct: 1201 QEPEKPNVSDSAEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGAVSWDQPYCRD 1260

Query: 1261 EHASSIPRRNLDQSGLSTSSGGSGGISSPQLAELIQNAVKEYASHGLPGEVGAFLDFVKS 1320
            EH S+  +R+LDQSG+STSS GS  ISSPQ +ELIQNAVKEY +HGLPGEVGAFLDFVKS
Sbjct: 1261 EHTSTTSKRDLDQSGVSTSSAGSVVISSPQFSELIQNAVKEYTNHGLPGEVGAFLDFVKS 1320

Query: 1321 HFPPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHENMHSKLNEDENFSKVASS 1380
            HFPPLR+IQVPGTSNVKGEKIIFSFDTLEEDIYLSV KEMH+ +HSK N+DE  SKVAS 
Sbjct: 1321 HFPPLRDIQVPGTSNVKGEKIIFSFDTLEEDIYLSVCKEMHDTLHSKFNDDEKISKVASG 1380

Query: 1381 LVTEDSKSVLHVKYIAALSRELAENHSTSESAHNVSMPVESLQPTTFNEIDPVDCDGIYL 1440
                DS+SVLHVKYIAALSRELAENHSTSESA N+ MPVESLQPT  NEI P DCDGIYL
Sbjct: 1381 ---GDSRSVLHVKYIAALSRELAENHSTSESARNIHMPVESLQPTILNEIGPTDCDGIYL 1440

Query: 1441 SSCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAFPR 1500
            SSCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRL+NSTLPAFPR
Sbjct: 1441 SSCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLSNSTLPAFPR 1500

Query: 1501 EFQKFGNPSMSSIGSLSHVSGQSNKSTEEVNPLYVQKAVAFLQSTAKAVGKNKVLKDISV 1560
            EFQK  +P  SS+G+LSHVSG  NKS E VNPLY+Q+AVA LQS AKAVGKN VLKDISV
Sbjct: 1501 EFQKIWSPRTSSVGTLSHVSGHLNKSNERVNPLYIQEAVALLQSAAKAVGKNNVLKDISV 1560

Query: 1561 HRHRKVSRNLEAVSLVLSKLYFSGKQDKSISCTRVNPSILMWDTLKYSLVSMEIAARSKT 1620
            HRH+KVSRNLEAVSLVLSKLYFS KQDK IS +RVNPSILMWDTLKYSLVSMEIAARSKT
Sbjct: 1561 HRHKKVSRNLEAVSLVLSKLYFSWKQDKLISSSRVNPSILMWDTLKYSLVSMEIAARSKT 1620

Query: 1621 DVNPNIGLNTLYKELKTSGGFILSLLLKVIQSIKCEDSLLLLQRFCGIQRFADSICSGVS 1680
            D+NP+IGLNTLYKELKTSGGF+LSLLLKVIQS+KCEDSLLLLQR CGIQRFADSICSG+S
Sbjct: 1621 DMNPSIGLNTLYKELKTSGGFVLSLLLKVIQSVKCEDSLLLLQRLCGIQRFADSICSGMS 1680

Query: 1681 NENANDSCGQGILHILTSLRVELPQFDIQFLSRGSDPVLAHDPFASVMWVLFCLPCPFLS 1740
            NENA+DSCG+GILHILTSLR ELPQFD QFLSRGSDPV+AHDPFAS+MWVLFCLP PFLS
Sbjct: 1681 NENASDSCGRGILHILTSLRSELPQFDSQFLSRGSDPVIAHDPFASLMWVLFCLPFPFLS 1740

Query: 1741 CKESLISLVHIFYLVSVAQGIIASCMKYQSKIDGMGFSDCLIADICKIMGESEYARRYFV 1800
            C+ESL+SLVHIFYLVSV Q II   +K Q ++DG+G SDCLI DICKIMGES YAR+YFV
Sbjct: 1741 CRESLLSLVHIFYLVSVTQAIITYFIKSQWEVDGLGSSDCLITDICKIMGESGYARQYFV 1800

Query: 1801 SDYTEPSCNVKDMIRSLTFTYLRRCALLLKLLNSSARAPIFDGETALERYLAVDDMIDNI 1860
            S+YTEPSCNVKD +R+LTF YLRRCALLL+LL+SSAR PIFDGETALE YL  ++MIDNI
Sbjct: 1801 SNYTEPSCNVKDTVRNLTFPYLRRCALLLQLLSSSARVPIFDGETALETYLVGNNMIDNI 1860

Query: 1861 ALELNEIGKLEKIFKIPPLDVVLKDEASRSLVWKWFCHFNREFEFQRSKNLKHCTPAVPF 1920
             +ELNE+ KL+K+F+IPPLD+VLKD  SR LV KWFCHFN+EFEFQR K +KHCTPAV F
Sbjct: 1861 TVELNEVEKLQKMFEIPPLDIVLKDRTSRLLVSKWFCHFNKEFEFQRFKIIKHCTPAVAF 1920

Query: 1921 QLIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANI 1980
            QLIRLPHVYHDLLQRYIKKRC DCKHVIDDPALCL+CG+LCSPSWKSCCRESGCQAHANI
Sbjct: 1921 QLIRLPHVYHDLLQRYIKKRCADCKHVIDDPALCLICGKLCSPSWKSCCRESGCQAHANI 1980

Query: 1981 CAAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSY 2040
            CAAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSY
Sbjct: 1981 CAAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSY 2036

BLAST of MS019737 vs. TAIR 10
Match: AT5G02310.1 (proteolysis 6 )

HSP 1 Score: 1794.6 bits (4647), Expect = 0.0e+00
Identity = 1009/2054 (49.12%), Postives = 1343/2054 (65.38%), Query Frame = 0

Query: 19   DRIVRRLALLGVPEELLDQLQPGLVSFVKDNKILIPQLVSAILPTDVEVAEVIRDAKAGS 78
            D ++ RLA +GVP++   + + GLV FV+ N   I +LVSA+LPTD +V   +++A+   
Sbjct: 17   DLVIERLASVGVPKKY--RSKRGLVEFVRANPAKISELVSALLPTDDDVKLGLKEARERP 76

Query: 79   KKSMAGPTMKNQFRESMVWLQWLMFEGEPTFALKNLSKMSFGQRGVCGAVWGHNDIAYRC 138
            +KS   PTMK +FRESM  LQWLMF+ EP  +L+NL+K++  QRGVCG+VWG NDIAYRC
Sbjct: 77   RKSAVSPTMKKRFRESMNMLQWLMFQDEPDVSLRNLAKLNLDQRGVCGSVWGQNDIAYRC 136

Query: 139  RTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVTAWKREGFCSKHKGAERIQ 198
            RTCE+DPTCAICVPCFQ+G+H  HDYSIIYTGGGCCDCGD TAWK +GFCS HKG+E+I+
Sbjct: 137  RTCENDPTCAICVPCFQNGDHNSHDYSIIYTGGGCCDCGDETAWKPDGFCSNHKGSEQIR 196

Query: 199  PLPEEFVESVGPILDALFTSWKNKLLSAEDISVEDPKLSDRVMEHKKVANELTFAVVEML 258
            PL E    SVGPILDALFT W NKLLSAE    +  + +D ++  +K++NELTF VVEML
Sbjct: 197  PLSENLANSVGPILDALFTCWNNKLLSAESSGQKGARSNDTLVILQKMSNELTFIVVEML 256

Query: 259  LDFCKYSESLLSFVSKRVISLAGFLDILVRLERLLTDIVVKKVHELLLKLLGEPIFKYEF 318
            L+F   SESLLSFVS+R+IS +G L IL++ ER L   V+KK+H+L LKL+G+P+FK EF
Sbjct: 257  LEFSMSSESLLSFVSRRIISSSGLLSILLKAERFLDQDVMKKLHDLFLKLIGDPVFKCEF 316

Query: 319  AKVFLNYYPTVISEAIEEGSDHALKKYPLLPTFSVQIFTVPTLTPRLVEEMNLLSILLGC 378
            AK F++YYP VISE +++G+D+A KKYPLL TFSVQI TVPTLTP LV+EMNLL++LLGC
Sbjct: 317  AKAFVSYYPVVISEVVKQGTDNAFKKYPLLSTFSVQILTVPTLTPFLVKEMNLLAMLLGC 376

Query: 379  LEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAVVPRYVIYEQQDILRTWLR 438
            L DIF+SC  EDG LQ  K   L ET+ RV+ D++FVMSHA+V +Y  +E +++ R+WL 
Sbjct: 377  LSDIFVSCSGEDGLLQATKLERLCETSERVIGDLKFVMSHAIVSKYATHEHRELSRSWLT 436

Query: 439  LLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHTLLVNEAFSAASSSSSSEET 498
            LLTF QGM+P KRETG+ I+EEN+ +HL F L HS+A IH+LLVN  +SAAS      + 
Sbjct: 437  LLTFAQGMNPLKRETGIPIDEENDYMHLFFVLGHSIAVIHSLLVNGTYSAASDEEIENDR 496

Query: 499  ADAMSFHAFKQNPDDMDGIRHAKVGRLSQESAACNVFGRSSTVTS--ASRVDEV--CSDT 558
                 F     +  D DG R+AKVGRLS E + C     SS+  S  AS V ++      
Sbjct: 497  NAKEEF-----DKCDGDGERYAKVGRLSHEDSVCTAIVSSSSFDSSMASEVHKIDPFHAL 556

Query: 559  ISSTIMWLTYECLKVIDSWLGTENVLGGFPNMFAENASMASSCKVYSLRKPSALES---- 618
            + S+ ++L  ECLKV+++ LG +  +  F             CK+ S    +  ES    
Sbjct: 557  LPSSAIYLIRECLKVLETCLGNDEGISKF------------LCKLSSSSGRNIPESKMSW 616

Query: 619  -RKISSKVEKGKFIFEKVAGKSKDHNRQYSSRIYSGLQ--MSIDNEHG-VSLGEDNQLMD 678
             R+    VE G  +   +A  S+D +   S  +   +Q  +S+DN  G   + + +   D
Sbjct: 617  PRRDLLNVETGGSVSSNLASSSRDPSTGLSP-LCGDIQTNLSLDNVCGPYGVVQTDVTAD 676

Query: 679  VTNDTVTVEDYSMETDALHFLSFSAWPNIVYDVSSQDISIHIPLHRLLSLLLQKALRSSF 738
                +    D +     L  L    WP+I YDVSSQ IS+H+PLHRLLSLL+QKALR  +
Sbjct: 677  SKRVSCNSADLTKNASGLRILGLCDWPDIHYDVSSQAISVHLPLHRLLSLLIQKALRICY 736

Query: 739  SESVVPSATGASSSNLSFEYVDFFKSVLTDYHPYGFSAFVMEHPLRIKVFCAEVNAGMWR 798
             ES   S  G S S+    + DFF SV+ D+HP GFSA VMEH L+I+VFCA+V AGMW+
Sbjct: 737  GESA--SYNGVSISH-EIPHADFFSSVIGDFHPCGFSALVMEHVLQIRVFCAQVIAGMWK 796

Query: 799  RNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPELYISRILERFRLLDYLSLNV 858
            +NGD+AL+SCE YRS+RWSEQ LELDLFLLQCCAA+AP + Y+ ++L RF L  YLSLN 
Sbjct: 797  KNGDSALVSCEWYRSVRWSEQGLELDLFLLQCCAALAPADSYVDKLLSRFGLSSYLSLNP 856

Query: 859  ERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQLVKALPR 918
            +  +EYEP+LVQEML L+IQ++ ERRFCGL+ AESL+RE+I+KLA GD THSQLVK+LPR
Sbjct: 857  DITNEYEPVLVQEMLGLLIQILQERRFCGLSTAESLRREIIFKLATGDFTHSQLVKSLPR 916

Query: 919  DLSKCHQLQEILDTIAVYSNPSGFNQGMYSLRWKYWKELDLYHPRWSLRDLQIAEERYLR 978
            DLSK  +LQE+LD ++VY NPSG NQG YSL+   WKELDLYHPRW  RDLQ AEER+ R
Sbjct: 917  DLSKSDELQEVLDDVSVYCNPSGMNQGKYSLQSSCWKELDLYHPRWQSRDLQSAEERFSR 976

Query: 979  SCGASALTSQLPKWTKIYPPFRGLARIATCKAVLQIIRAVLFYSVFTDKSTKSRAPDSVL 1038
             CG SALT+QLP+W  IYPP +GLARI TCKA  QII + L+Y++ +  S KSRAPD VL
Sbjct: 977  YCGVSALTTQLPRWRMIYPPLKGLARIGTCKATFQIISSALYYALQSGTSVKSRAPDGVL 1036

Query: 1039 LTALHLLALALDICFQRKESSDQSFCSEDSIPLLLFADEEVDEGLTYGLGRQSLLSLLIL 1098
            +TAL LL+L+LDIC Q+++S+ Q  C E+SIP+L  A  E+  G+  G  ++SLLSLL+ 
Sbjct: 1037 ITALQLLSLSLDICTQQRQSNSQDCCLENSIPILELAGLEI-IGIAQGTEKESLLSLLVS 1096

Query: 1099 LMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDSRCMGKIRQHAPEILGYLSQSLPS 1158
            LMK    +GR    E GSCN+SS + +LLKKFS IDS CM  ++  APE++G       S
Sbjct: 1097 LMKTRMGDGRHQFPEPGSCNISSWIGNLLKKFSAIDSVCMNLLQSLAPEVVG------QS 1156

Query: 1159 SDPNSLSETSDSDKLKAKARERQAAILEKMRAEQYKFLASVDSSVEDD---AEFGQESEK 1218
                 +S ++  +K KAKA+ERQAAI+ KM+AEQ KFL+++ SS++DD   +EF + S+ 
Sbjct: 1157 GFDKVMSGSTSDEKRKAKAKERQAAIMAKMKAEQSKFLSTLSSSMDDDDPRSEF-ETSDS 1216

Query: 1219 PSVSDSAEQSETVCSLCHDSSSSVPISYLILLQKSKLVSLIDRGTVSWDQPYWRDEHASS 1278
                DS      VCSLCHD  S  P+S+LI LQKSKL+S +DRG  SWDQ    ++  S 
Sbjct: 1217 VMEHDSEIAVREVCSLCHDPDSKDPVSFLIFLQKSKLLSFVDRGPPSWDQCPQSEKKISV 1276

Query: 1279 IPRRNLDQSGLSTSSGGSGGISSPQLAELIQNAVKEYASHGLPGEVGAFLDFVKSHFPPL 1338
                +L +     +S  S  ISSP + +L  + + E A+          ++ +K+    +
Sbjct: 1277 DGAPDLLR---MNASSDSLRISSPLMLQLSDDTISESAN---------MIESIKARL--I 1336

Query: 1339 RNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHENMHSKLNEDENFSKVASSLVTED 1398
             N Q    S+    K   + ++LE  +Y +VR ++   ++  L   ++    A +   ++
Sbjct: 1337 GNGQTEKRSSDGRGKDESNMESLEIAMYQTVRNKIENMINQSLTRVDHQPHEAENCSEKN 1396

Query: 1399 S---KSVLHVKYIAALSRELAENHSTSESAHNVSMPVESLQPTTFNEIDPVDCDGIYLSS 1458
            S    S L  ++    SR+ +          +                 P+DCDG+YLSS
Sbjct: 1397 SVGGPSTLQGRFPDIRSRQTSRRPDAGSDGFH-----------------PIDCDGVYLSS 1456

Query: 1459 CGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAFPREF 1518
            CGHAVHQ CL+RYL SLKER  RR VFEG HIVD ++ EFLCPVCRRLANS LP  P + 
Sbjct: 1457 CGHAVHQSCLERYLKSLKERSGRRTVFEGAHIVDLKKKEFLCPVCRRLANSVLPECPGD- 1516

Query: 1519 QKFGNPSMSSIGSLSHVSGQSNKSTEEVNP-LYVQKAVAFLQSTAKAVGKNKVLKDISVH 1578
                   + S+  L        +  + + P L++ +A+  L+S A+ +      K ++  
Sbjct: 1517 -------LCSVSKLQDSPRTKLRRKDALQPSLWLSEALCLLRSAAEVIEDGDRGKTVTPQ 1576

Query: 1579 RHRKVSRNLEAVSLVLSKLYFSGKQDKSISCTRVNP-SILMWDTLKYSLVSMEIAAR-SK 1638
                  ++L++VS +L   YF   +DK++    + P SI+MWDTLKYSL+SMEI  R +K
Sbjct: 1577 GDGPRRKDLKSVSKMLWDFYFPKPEDKTLKRLWLPPQSIVMWDTLKYSLISMEIGTRFAK 1636

Query: 1639 TDVNPNIGLNTLYKELKTSGGFILSLLLKVIQSIKCEDSLLLLQRFCGIQRFADSICSGV 1698
              + P   +++LY+ELKTS G ILS+LL+V+QS + ++++ + QRF G++  A+SIC GV
Sbjct: 1637 NSMLPVYCIDSLYEELKTSKGTILSVLLRVVQSSRTKNTIHVRQRFVGMKHLAESICYGV 1696

Query: 1699 SNENANDSCGQGILHILTSLRVELPQFDIQFLSRGSDPVLAHDPFASVMWVLFCLPCPFL 1758
            S+ +++   G             L   D+ + +R SDPVLAHDPF+S+MW LFCLP PFL
Sbjct: 1697 SSSSSSSIFGS------EGTTGSLKNIDLLW-NRASDPVLAHDPFSSLMWALFCLPFPFL 1756

Query: 1759 SCKESLISLVHIFYLVSVAQGIIASCMKYQSKIDGMGFSDCLIADICKIMGESEYARRYF 1818
            +C+ESL+SLVHIF+ VS+ Q +IA C    S++  + F + L+ DI   + ES     YF
Sbjct: 1757 TCEESLLSLVHIFHSVSLVQTVIAYCACRPSELSELNFGENLLNDISNALRESG-GWEYF 1816

Query: 1819 VSDYTEPSCNVKDMIRSLTFTYLRRCALLLKLLNSSAR-----APIFDGETALERYLAVD 1878
             S+  + SC++KD IR  +  +LRRCALL KLL S+ R     + +FD        L  D
Sbjct: 1817 RSNNMDLSCDIKDTIRKYSLPFLRRCALLWKLLKSTPRKLHEESDMFD--------LPSD 1876

Query: 1879 DMIDNIAL------ELNEIGKLEKIFKIPPLDVVLKDEASRSLVWKWFCHFNREFEFQRS 1938
               DN+        ELN + +LEK+F IPP+D++L DE  RS    W  HF RE+   R 
Sbjct: 1877 PTTDNMDFIYSPQSELNHVQELEKMFNIPPIDIILNDELLRSSTQIWLQHFQREYRVNRV 1936

Query: 1939 KNLKHCTPAVPFQLIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSC 1998
            K     TP VPFQL++LP++Y DLLQR IKKRC +C  VI++P LCLLCG LCSP W  C
Sbjct: 1937 KRSLCITPVVPFQLMKLPNLYQDLLQRCIKKRCVNCTKVIEEPVLCLLCGSLCSPIWSPC 1984

Query: 1999 CRESGCQAHANICAAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPL 2041
            CRESGC  HA  C AGTGVFLLIRRTTILLQR ARQ+PWPSPYLD FGEEDI+M RGK L
Sbjct: 1997 CRESGCPNHAITCGAGTGVFLLIRRTTILLQRFARQSPWPSPYLDTFGEEDIDMIRGKRL 1984