MS019624 (gene) Bitter gourd (TR) v1

BLAST of MS019624 vs. NCBI nr
Match: XP_038905012.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida])

HSP 1 Score: 959.5 bits (2479), Expect = 1.3e-275
Identity = 492/576 (85.42%), Postives = 521/576 (90.45%), Query Frame = 0

Query: 1   MAASSFSSGNAFWYQLLFGRWFSVFASVLIMSVSGATYMFGLYSSDIKSSLAYDQTTLNL 60
           MAAS+ +S N FWYQ LFGRWFSVFAS+LIMSV+GATYMFGLYSSDIKSSL YDQTTLNL
Sbjct: 1   MAASNPTSANPFWYQFLFGRWFSVFASILIMSVAGATYMFGLYSSDIKSSLGYDQTTLNL 60

Query: 61  LSFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWLAVTARIPNPQIWQMCL 120
           LSFFKDLGGNVGVI GLINEVAP WVVLLIGAVMNLFGYTMIWLAVT RIP PQIW MCL
Sbjct: 61  LSFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCL 120

Query: 121 YICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAVLTQLYHAFYGNNSKS 180
           YICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGA+L+QL+HAFYGNNSKS
Sbjct: 121 YICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNNSKS 180

Query: 181 LILLIAWLPAAVSVAFLRFVRIIKDLRQPNELRVFYHFLYISLGLAGSLMVLIIGQNRLR 240
           LI LIAWLPAAVSV FLRFVRIIKDLRQPNEL+VFYH LYISLGLAGSLMVLII QNRLR
Sbjct: 181 LIFLIAWLPAAVSVTFLRFVRIIKDLRQPNELKVFYHILYISLGLAGSLMVLIILQNRLR 240

Query: 241 FQQMEYAGSACIVLVLLLLPLAVVFREELSVWKSKIENPISQLELASQQSPPLPP--PSS 300
           FQQ++Y GSA +V+VLLLLPLA+VFREEL+VWKSKI NPI QLELASQQ P  PP  P S
Sbjct: 241 FQQIQYVGSAIVVIVLLLLPLAIVFREELNVWKSKIANPILQLELASQQPPRPPPLAPPS 300

Query: 301 PQSDSCVQNVFKPPNRGEDYTILQAVFSVDMMILFVATICGVGGTLTAIDNLGQIGESLG 360
           P S SC +N FKPPNRGEDYTI QA+FSVDM+ILF+ATICGVGGTLTAIDNLGQIGESLG
Sbjct: 301 PPSGSCFKNTFKPPNRGEDYTIPQAIFSVDMIILFIATICGVGGTLTAIDNLGQIGESLG 360

Query: 361 YPSQSTTTFISLVSIWNYLGRVVAGFASEFLWKKYKIPRPLFFFITLLLSCVGHLLIAFG 420
           YPS STTTFISLVSIWNYLGRVV+GF SE+ WKKYK+PRPLF F TL+LSCVGHLLIAFG
Sbjct: 361 YPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLIAFG 420

Query: 421 VPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNVRVAG 480
           VPNSLYFSSI++GFCFGAQWPLIFAIISEIFGLKYYATLYNLGG ASPIGAYILNVRVAG
Sbjct: 421 VPNSLYFSSIVVGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGAASPIGAYILNVRVAG 480

Query: 481 NLYDREARRQMEAAGLRRKTGEDLRCVGVECYRMAFLIITAATVFGGLVSLILVVRTWKF 540
           +LYDREA+RQMEAAG RR  GEDL C+GVECYR AFLIITAATVFGGLVSLILVVRTWKF
Sbjct: 481 HLYDREAQRQMEAAGRRRNIGEDLSCLGVECYRKAFLIITAATVFGGLVSLILVVRTWKF 540

Query: 541 YKGDIYSKFREEEDGATEGSKMAAPNSTMPAAKTES 575
           YK DIY KFREEE    E   +   N  + AAKTES
Sbjct: 541 YKSDIYRKFREEEAEDVEIKIVTPANGALTAAKTES 576

BLAST of MS019624 vs. NCBI nr
Match: XP_023540992.1 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023540993.1 protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_023540994.1 protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 953.4 bits (2463), Expect = 9.2e-274
Identity = 490/578 (84.78%), Postives = 520/578 (89.97%), Query Frame = 0

Query: 1   MAASSFSSGNAFWYQLLFGRWFSVFASVLIMSVSGATYMFGLYSSDIKSSLAYDQTTLNL 60
           MAAS+ S+ +  WYQ L GRWFSVFAS+LIMSVSGATYMFGLYSSDIKSSL YDQTTLNL
Sbjct: 1   MAASNPSTVHPIWYQFLLGRWFSVFASILIMSVSGATYMFGLYSSDIKSSLGYDQTTLNL 60

Query: 61  LSFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWLAVTARIPNPQIWQMCL 120
           LSFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIW+AVT+RIP PQIW MCL
Sbjct: 61  LSFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWMAVTSRIPKPQIWHMCL 120

Query: 121 YICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAVLTQLYHAFYGNNSKS 180
           YICIGANSQTFANTGALVT VKNFPESRGSVLGLLKGFVGLSGA+LTQLYHAFYGNNSKS
Sbjct: 121 YICIGANSQTFANTGALVTSVKNFPESRGSVLGLLKGFVGLSGAILTQLYHAFYGNNSKS 180

Query: 181 LILLIAWLPAAVSVAFLRFVRIIKDLRQPNELRVFYHFLYISLGLAGSLMVLIIGQNRLR 240
           LILLIAWLPAAVSV FLRFVR+IKDLRQPNELRVF+H LYISLGLAGSLMVLII QNRL+
Sbjct: 181 LILLIAWLPAAVSVTFLRFVRLIKDLRQPNELRVFFHILYISLGLAGSLMVLIILQNRLK 240

Query: 241 FQQMEYAGSACIVLVLLLLPLAVVFREELSVWKSKIENPISQLELASQQSPP------LP 300
           F QMEYAGSA +V+ LLLLPLA+V+REELSVWK+KIE+P+SQLELASQQ PP       P
Sbjct: 241 FHQMEYAGSAIVVIALLLLPLAIVYREELSVWKTKIESPLSQLELASQQQPPPLTSAAPP 300

Query: 301 PPSSPQSDSCVQNVFKPPNRGEDYTILQAVFSVDMMILFVATICGVGGTLTAIDNLGQIG 360
            P SP S SC +N FKPPNRGEDYTI QAVFS+DM+ILF+ATICGVGGTLTAIDNLGQIG
Sbjct: 301 APPSPPSGSCFENTFKPPNRGEDYTIPQAVFSIDMIILFIATICGVGGTLTAIDNLGQIG 360

Query: 361 ESLGYPSQSTTTFISLVSIWNYLGRVVAGFASEFLWKKYKIPRPLFFFITLLLSCVGHLL 420
           ESL YPSQSTTTFISLVSIWNYLGRVVAGF SEFLWKKYK+PRPL FF+T++LSCVGHLL
Sbjct: 361 ESLRYPSQSTTTFISLVSIWNYLGRVVAGFLSEFLWKKYKVPRPLIFFVTMILSCVGHLL 420

Query: 421 IAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNV 480
           IAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNV
Sbjct: 421 IAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNV 480

Query: 481 RVAGNLYDREARRQMEAAGLRRKTGEDLRCVGVECYRMAFLIITAATVFGGLVSLILVVR 540
           RVAG+LYDREA RQMEAAG RR  GEDL C+GVECYR AFLIITAATV G  VSLILVVR
Sbjct: 481 RVAGHLYDREATRQMEAAGRRRNIGEDLNCLGVECYRKAFLIITAATVLGAFVSLILVVR 540

Query: 541 TWKFYKGDIYSKFREEEDGATEGSKMAAPNSTMPAAKT 573
           TWKFYKGDIY KFREE     E    A  NS +  ++T
Sbjct: 541 TWKFYKGDIYRKFREEAGEEVEVQMAAPTNSAVAKSQT 578

BLAST of MS019624 vs. NCBI nr
Match: XP_023005202.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 951.4 bits (2458), Expect = 3.5e-273
Identity = 491/578 (84.95%), Postives = 521/578 (90.14%), Query Frame = 0

Query: 1   MAASSFSSGNAFWYQLLFGRWFSVFASVLIMSVSGATYMFGLYSSDIKSSLAYDQTTLNL 60
           MAAS+ S+ +  WYQ L GRWFSVFAS+LIMSVSGATYMFGLYSSDIKSSL YDQTTLNL
Sbjct: 1   MAASNPSTVHPIWYQFLLGRWFSVFASILIMSVSGATYMFGLYSSDIKSSLGYDQTTLNL 60

Query: 61  LSFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWLAVTARIPNPQIWQMCL 120
           LSFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIW+AVT+RIP PQIW MCL
Sbjct: 61  LSFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWMAVTSRIPKPQIWHMCL 120

Query: 121 YICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAVLTQLYHAFYGNNSKS 180
           YICIGANSQTFANTGALVT VKNFPESRGSVLGLLKGFVGLSGA+LTQLYHAFYGNNSKS
Sbjct: 121 YICIGANSQTFANTGALVTSVKNFPESRGSVLGLLKGFVGLSGAILTQLYHAFYGNNSKS 180

Query: 181 LILLIAWLPAAVSVAFLRFVRIIKDLRQPNELRVFYHFLYISLGLAGSLMVLIIGQNRLR 240
           LILLIAWLPAAVSV FLRFVR+IKDLRQPNELRVF+H LYISLGLAGSLMVLII QNRL+
Sbjct: 181 LILLIAWLPAAVSVTFLRFVRLIKDLRQPNELRVFFHILYISLGLAGSLMVLIILQNRLK 240

Query: 241 FQQMEYAGSACIVLVLLLLPLAVVFREELSVWKSKIENPISQLELASQ-QSPPLP----- 300
           F QMEYAGSA +V+ LLLLPLA+V+REELSVWK+KIE+PISQLELASQ Q PPL      
Sbjct: 241 FHQMEYAGSAIVVIALLLLPLAIVYREELSVWKTKIESPISQLELASQPQPPPLTSAAPV 300

Query: 301 PPSSPQSDSCVQNVFKPPNRGEDYTILQAVFSVDMMILFVATICGVGGTLTAIDNLGQIG 360
            P SP S SC +N FKPPNRGEDYTILQAVFS+DM+ILF+ATICGVGGTLTAIDNLGQIG
Sbjct: 301 APPSPPSGSCFENTFKPPNRGEDYTILQAVFSIDMIILFIATICGVGGTLTAIDNLGQIG 360

Query: 361 ESLGYPSQSTTTFISLVSIWNYLGRVVAGFASEFLWKKYKIPRPLFFFITLLLSCVGHLL 420
           ESL YPSQSTTTFISLVSIWNYLGRVVAGF SEFLWKKYK+PRPL FF+T++LSCVGHLL
Sbjct: 361 ESLRYPSQSTTTFISLVSIWNYLGRVVAGFLSEFLWKKYKVPRPLIFFVTMILSCVGHLL 420

Query: 421 IAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNV 480
           IAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNV
Sbjct: 421 IAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNV 480

Query: 481 RVAGNLYDREARRQMEAAGLRRKTGEDLRCVGVECYRMAFLIITAATVFGGLVSLILVVR 540
           RVAG+LYDREA RQMEAAG RR  GEDL C+GVECYR AFLIITAATV G  VSLILVVR
Sbjct: 481 RVAGHLYDREATRQMEAAGRRRNIGEDLNCLGVECYRKAFLIITAATVLGAFVSLILVVR 540

Query: 541 TWKFYKGDIYSKFREEEDGATEGSKMAAPNSTMPAAKT 573
           TWKFYKGDIY KFRE+     E    A  NS +  ++T
Sbjct: 541 TWKFYKGDIYQKFREDSGEEVEVQMAAPTNSAVAKSQT 578

BLAST of MS019624 vs. NCBI nr
Match: XP_023522337.1 (protein NUCLEAR FUSION DEFECTIVE 4-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 950.7 bits (2456), Expect = 6.0e-273
Identity = 485/556 (87.23%), Postives = 512/556 (92.09%), Query Frame = 0

Query: 1   MAASSFSSGNAFWYQLLFGRWFSVFASVLIMSVSGATYMFGLYSSDIKSSLAYDQTTLNL 60
           MAAS+ S+ +  WYQ L GRWFSVFAS+LIMSVSGATYMFGLYSSDIKSSL YDQTTLNL
Sbjct: 1   MAASNPSTVHPIWYQFLLGRWFSVFASILIMSVSGATYMFGLYSSDIKSSLGYDQTTLNL 60

Query: 61  LSFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWLAVTARIPNPQIWQMCL 120
           LSFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIW+AVT+RIP PQIW MCL
Sbjct: 61  LSFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWMAVTSRIPKPQIWHMCL 120

Query: 121 YICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAVLTQLYHAFYGNNSKS 180
           YICIGANSQTFANTGALVT VKNFPESRGSVLGLLKGFVGLSGA+LTQLYHAFYGNNSKS
Sbjct: 121 YICIGANSQTFANTGALVTSVKNFPESRGSVLGLLKGFVGLSGAILTQLYHAFYGNNSKS 180

Query: 181 LILLIAWLPAAVSVAFLRFVRIIKDLRQPNELRVFYHFLYISLGLAGSLMVLIIGQNRLR 240
           LILLIAWLPAAVSV FLRFVR+IKDLRQPNELRVF+H LYISLGLAGSLMVLII QNRL+
Sbjct: 181 LILLIAWLPAAVSVTFLRFVRLIKDLRQPNELRVFFHILYISLGLAGSLMVLIILQNRLK 240

Query: 241 FQQMEYAGSACIVLVLLLLPLAVVFREELSVWKSKIENPISQLELASQQSPP------LP 300
           F QMEYAGSA +V+ LLLLPLA+V+REELSVWK+KIE+P+SQLELASQQ PP       P
Sbjct: 241 FHQMEYAGSAIVVIALLLLPLAIVYREELSVWKTKIESPLSQLELASQQQPPPLTSAAPP 300

Query: 301 PPSSPQSDSCVQNVFKPPNRGEDYTILQAVFSVDMMILFVATICGVGGTLTAIDNLGQIG 360
            P SP S SC +N FKPPNRGEDYTI QAVFS+DM+ILF+ATICGVGGTLTAIDNLGQIG
Sbjct: 301 APPSPPSGSCFENTFKPPNRGEDYTIPQAVFSIDMIILFIATICGVGGTLTAIDNLGQIG 360

Query: 361 ESLGYPSQSTTTFISLVSIWNYLGRVVAGFASEFLWKKYKIPRPLFFFITLLLSCVGHLL 420
           ESL YPSQSTTTFISLVSIWNYLGRVVAGF SEFLWKKYK+PRPL FF+T++LSCVGHLL
Sbjct: 361 ESLRYPSQSTTTFISLVSIWNYLGRVVAGFLSEFLWKKYKVPRPLIFFVTMILSCVGHLL 420

Query: 421 IAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNV 480
           IAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNV
Sbjct: 421 IAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNV 480

Query: 481 RVAGNLYDREARRQMEAAGLRRKTGEDLRCVGVECYRMAFLIITAATVFGGLVSLILVVR 540
           RVAG+LYDREA RQMEAAG RR  GEDL C+GVECYR AFLIITAATV G  VSLILVVR
Sbjct: 481 RVAGHLYDREATRQMEAAGRRRNIGEDLNCLGVECYRKAFLIITAATVLGAFVSLILVVR 540

Query: 541 TWKFYKGDIYSKFREE 551
           TWKFYKGDIY KFREE
Sbjct: 541 TWKFYKGDIYRKFREE 556

BLAST of MS019624 vs. NCBI nr
Match: KAG6596491.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 950.3 bits (2455), Expect = 7.8e-273
Identity = 490/579 (84.63%), Postives = 521/579 (89.98%), Query Frame = 0

Query: 1   MAASSFSSGNAFWYQLLFGRWFSVFASVLIMSVSGATYMFGLYSSDIKSSLAYDQTTLNL 60
           MAAS+ S+ +  WYQ L GRWFSVFAS+LIMSVSGATYMFGLYSSDIKSSL YDQTTLNL
Sbjct: 1   MAASNPSTVHPIWYQFLLGRWFSVFASILIMSVSGATYMFGLYSSDIKSSLGYDQTTLNL 60

Query: 61  LSFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWLAVTARIPNPQIWQMCL 120
           LSFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIW+AVT+RIP PQIW MCL
Sbjct: 61  LSFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWMAVTSRIPKPQIWHMCL 120

Query: 121 YICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAVLTQLYHAFYGNNSKS 180
           YICIGANSQTFANTGALVT VKNFPESRGSVLGLLKGFVGLSGA+LTQLYHAFYGNNSKS
Sbjct: 121 YICIGANSQTFANTGALVTSVKNFPESRGSVLGLLKGFVGLSGAILTQLYHAFYGNNSKS 180

Query: 181 LILLIAWLPAAVSVAFLRFVRIIKDLRQPNELRVFYHFLYISLGLAGSLMVLIIGQNRLR 240
           LILLIAWLPAAVSV FLRFVR+IKDLRQPNELRVF+H LYISLGLAGSLMVLII QNRL+
Sbjct: 181 LILLIAWLPAAVSVTFLRFVRLIKDLRQPNELRVFFHILYISLGLAGSLMVLIILQNRLK 240

Query: 241 FQQMEYAGSACIVLVLLLLPLAVVFREELSVWKSKIENPISQLELASQQSP-------PL 300
           F QMEYAGSA +V+ LLLLPLA+V+REELSVWK+KIE+P+SQLELASQQ P       PL
Sbjct: 241 FHQMEYAGSAIVVIALLLLPLAIVYREELSVWKTKIESPLSQLELASQQQPPPLTSAAPL 300

Query: 301 PPPSSPQSDSCVQNVFKPPNRGEDYTILQAVFSVDMMILFVATICGVGGTLTAIDNLGQI 360
            PP SP S SC +N FKPPNRGEDYTI QAVFS+DM+ILF+ATICGVGGTLTAIDNLGQI
Sbjct: 301 APP-SPPSGSCFENTFKPPNRGEDYTIPQAVFSIDMIILFIATICGVGGTLTAIDNLGQI 360

Query: 361 GESLGYPSQSTTTFISLVSIWNYLGRVVAGFASEFLWKKYKIPRPLFFFITLLLSCVGHL 420
           GESL YPSQSTTTFISLVSIWNYLGRVVAGF SEFLWKKYK+PRPL FF+T++LSCVGHL
Sbjct: 361 GESLRYPSQSTTTFISLVSIWNYLGRVVAGFLSEFLWKKYKVPRPLIFFVTMILSCVGHL 420

Query: 421 LIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILN 480
           LIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILN
Sbjct: 421 LIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILN 480

Query: 481 VRVAGNLYDREARRQMEAAGLRRKTGEDLRCVGVECYRMAFLIITAATVFGGLVSLILVV 540
           V+VAG+LYDREA RQMEAAG RR  GEDL C+GVECYR AFLIITAATV G  VSLILVV
Sbjct: 481 VKVAGHLYDREATRQMEAAGRRRNIGEDLNCLGVECYRKAFLIITAATVLGAFVSLILVV 540

Query: 541 RTWKFYKGDIYSKFREEEDGATEGSKMAAPNSTMPAAKT 573
           RTWKFYKGDIY KFREE     E    A  NS +  ++T
Sbjct: 541 RTWKFYKGDIYRKFREEAGEEVEVQMAAPTNSAVAKSQT 578

BLAST of MS019624 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 1.4e-30
Identity = 134/540 (24.81%), Postives = 250/540 (46.30%), Query Frame = 0

Query: 17  LFGRWFS---VFASVLIMSVSGATYMFGLYSSDIKSSLAYDQTTLNLLSFFKDLGGNVGV 76
           +FGRW     + A++ I + +G  + F  YSS +KS L   Q  LN L+   DLG   G 
Sbjct: 37  MFGRWRKWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGW 96

Query: 77  IGGLINEVAPPWVVLLIGAVMNLFGYTMIWLAVTARIPNPQIWQMCLYICIGAN-SQTFA 136
             G+     P  VVL   A M   GY + WL +T  I  P  + +    C+ A  S  + 
Sbjct: 97  SSGIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP--YSLVFLCCLLAGLSICWF 156

Query: 137 NTGALVTCVKNFPESRGSVLGLLKGFVGLSGAVLTQLYHAFYGNNSKSLILLIAWLPAAV 196
           NT   + C+++FP +R   L L   F G+S A+ +  ++A   ++S   +LL + +P  V
Sbjct: 157 NTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVV 216

Query: 197 SVAFLRFVRIIKDLRQPNELRVFYH----FLYISLGLAGSLMVLIIGQNRLRFQQMEYAG 256
           S A L  V     L    +     H    F  +++    +   L++  +     ++ + G
Sbjct: 217 SFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIG 276

Query: 257 SACIVLVLLLLPLAVVFREE-LSVWKSKIENPISQL------ELASQQSPPLPPPSSPQS 316
           +  +++  L  PL V  R+  L V  +++ +  S        EL +Q++           
Sbjct: 277 AVVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHM 336

Query: 317 DSCVQNVFKPPNRGEDYTILQAVFSVDMMILFVATICGVGGTLTAIDNLGQIGESLGYPS 376
            +  +        G++++    +  ++  + ++A  CG    L   +NLGQI +SLG   
Sbjct: 337 GTAKEG--NTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLG--- 396

Query: 377 QSTTTFISLVSIWNYLGRVVAGFASEFLWKKYKIPRPLFFFITLLLSCVGHLLIAF--GV 436
           Q++TT +++ S +++ GR+++  A +F+ K++++ R  +F I LL + +   L+A     
Sbjct: 397 QNSTTLVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQ 456

Query: 437 PNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNVRVAGN 496
             +L  ++ +IG   G  +    +I S++FG       +N+     PIG+ +L   +A +
Sbjct: 457 QTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGS-LLYGYIAAS 516

Query: 497 LYDREARRQMEAAGLRRKTGEDLRCVGVECYRMAFLIITAATVFGGLVSLILVVRTWKFY 540
           +Y+  A     +  +     + + C+G +CY   F+     ++ G + SL L +RT   Y
Sbjct: 517 IYEANA-----SPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of MS019624 vs. ExPASy TrEMBL
Match: A0A6J1KSH4 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111498302 PE=4 SV=1)

HSP 1 Score: 951.4 bits (2458), Expect = 1.7e-273
Identity = 491/578 (84.95%), Postives = 521/578 (90.14%), Query Frame = 0

Query: 1   MAASSFSSGNAFWYQLLFGRWFSVFASVLIMSVSGATYMFGLYSSDIKSSLAYDQTTLNL 60
           MAAS+ S+ +  WYQ L GRWFSVFAS+LIMSVSGATYMFGLYSSDIKSSL YDQTTLNL
Sbjct: 1   MAASNPSTVHPIWYQFLLGRWFSVFASILIMSVSGATYMFGLYSSDIKSSLGYDQTTLNL 60

Query: 61  LSFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWLAVTARIPNPQIWQMCL 120
           LSFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIW+AVT+RIP PQIW MCL
Sbjct: 61  LSFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWMAVTSRIPKPQIWHMCL 120

Query: 121 YICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAVLTQLYHAFYGNNSKS 180
           YICIGANSQTFANTGALVT VKNFPESRGSVLGLLKGFVGLSGA+LTQLYHAFYGNNSKS
Sbjct: 121 YICIGANSQTFANTGALVTSVKNFPESRGSVLGLLKGFVGLSGAILTQLYHAFYGNNSKS 180

Query: 181 LILLIAWLPAAVSVAFLRFVRIIKDLRQPNELRVFYHFLYISLGLAGSLMVLIIGQNRLR 240
           LILLIAWLPAAVSV FLRFVR+IKDLRQPNELRVF+H LYISLGLAGSLMVLII QNRL+
Sbjct: 181 LILLIAWLPAAVSVTFLRFVRLIKDLRQPNELRVFFHILYISLGLAGSLMVLIILQNRLK 240

Query: 241 FQQMEYAGSACIVLVLLLLPLAVVFREELSVWKSKIENPISQLELASQ-QSPPLP----- 300
           F QMEYAGSA +V+ LLLLPLA+V+REELSVWK+KIE+PISQLELASQ Q PPL      
Sbjct: 241 FHQMEYAGSAIVVIALLLLPLAIVYREELSVWKTKIESPISQLELASQPQPPPLTSAAPV 300

Query: 301 PPSSPQSDSCVQNVFKPPNRGEDYTILQAVFSVDMMILFVATICGVGGTLTAIDNLGQIG 360
            P SP S SC +N FKPPNRGEDYTILQAVFS+DM+ILF+ATICGVGGTLTAIDNLGQIG
Sbjct: 301 APPSPPSGSCFENTFKPPNRGEDYTILQAVFSIDMIILFIATICGVGGTLTAIDNLGQIG 360

Query: 361 ESLGYPSQSTTTFISLVSIWNYLGRVVAGFASEFLWKKYKIPRPLFFFITLLLSCVGHLL 420
           ESL YPSQSTTTFISLVSIWNYLGRVVAGF SEFLWKKYK+PRPL FF+T++LSCVGHLL
Sbjct: 361 ESLRYPSQSTTTFISLVSIWNYLGRVVAGFLSEFLWKKYKVPRPLIFFVTMILSCVGHLL 420

Query: 421 IAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNV 480
           IAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNV
Sbjct: 421 IAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNV 480

Query: 481 RVAGNLYDREARRQMEAAGLRRKTGEDLRCVGVECYRMAFLIITAATVFGGLVSLILVVR 540
           RVAG+LYDREA RQMEAAG RR  GEDL C+GVECYR AFLIITAATV G  VSLILVVR
Sbjct: 481 RVAGHLYDREATRQMEAAGRRRNIGEDLNCLGVECYRKAFLIITAATVLGAFVSLILVVR 540

Query: 541 TWKFYKGDIYSKFREEEDGATEGSKMAAPNSTMPAAKT 573
           TWKFYKGDIY KFRE+     E    A  NS +  ++T
Sbjct: 541 TWKFYKGDIYQKFREDSGEEVEVQMAAPTNSAVAKSQT 578

BLAST of MS019624 vs. ExPASy TrEMBL
Match: A0A6J1G7M8 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111451560 PE=4 SV=1)

HSP 1 Score: 950.3 bits (2455), Expect = 3.8e-273
Identity = 490/579 (84.63%), Postives = 521/579 (89.98%), Query Frame = 0

Query: 1   MAASSFSSGNAFWYQLLFGRWFSVFASVLIMSVSGATYMFGLYSSDIKSSLAYDQTTLNL 60
           MAAS+ S+ +  WYQ L GRWFSVFAS+LIMSVSGATYMFGLYSSDIKSSL YDQTTLNL
Sbjct: 1   MAASNPSTVHPIWYQFLLGRWFSVFASILIMSVSGATYMFGLYSSDIKSSLGYDQTTLNL 60

Query: 61  LSFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWLAVTARIPNPQIWQMCL 120
           LSFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIW+AVT+RIP PQIW MCL
Sbjct: 61  LSFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWMAVTSRIPKPQIWHMCL 120

Query: 121 YICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAVLTQLYHAFYGNNSKS 180
           YICIGANSQTFANTGALVT VKNFPESRGSVLGLLKGFVGLSGA+LTQLYHAFYGNNSKS
Sbjct: 121 YICIGANSQTFANTGALVTSVKNFPESRGSVLGLLKGFVGLSGAILTQLYHAFYGNNSKS 180

Query: 181 LILLIAWLPAAVSVAFLRFVRIIKDLRQPNELRVFYHFLYISLGLAGSLMVLIIGQNRLR 240
           LILLIAWLPAAVSV FLRFVR+IKDLRQPNELRVF+H LYISLGLAGSLMVLII QNRL+
Sbjct: 181 LILLIAWLPAAVSVTFLRFVRLIKDLRQPNELRVFFHILYISLGLAGSLMVLIILQNRLK 240

Query: 241 FQQMEYAGSACIVLVLLLLPLAVVFREELSVWKSKIENPISQLELASQQSP-------PL 300
           F QMEYAGSA +V+ LLLLPLA+V+REELSVWK+KIE+P+SQLELASQQ P       P+
Sbjct: 241 FHQMEYAGSAIVVIALLLLPLAIVYREELSVWKTKIESPLSQLELASQQQPPPLTSAAPV 300

Query: 301 PPPSSPQSDSCVQNVFKPPNRGEDYTILQAVFSVDMMILFVATICGVGGTLTAIDNLGQI 360
            PP SP S SC +N FKPPNRGEDYTI QAVFS+DM+ILF+ATICGVGGTLTAIDNLGQI
Sbjct: 301 APP-SPPSGSCFENTFKPPNRGEDYTIPQAVFSIDMIILFIATICGVGGTLTAIDNLGQI 360

Query: 361 GESLGYPSQSTTTFISLVSIWNYLGRVVAGFASEFLWKKYKIPRPLFFFITLLLSCVGHL 420
           GESL YPSQSTTTFISLVSIWNYLGRVVAGF SEFLWKKYK+PRPL FF+T++LSCVGHL
Sbjct: 361 GESLRYPSQSTTTFISLVSIWNYLGRVVAGFLSEFLWKKYKVPRPLIFFVTMILSCVGHL 420

Query: 421 LIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILN 480
           LIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILN
Sbjct: 421 LIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILN 480

Query: 481 VRVAGNLYDREARRQMEAAGLRRKTGEDLRCVGVECYRMAFLIITAATVFGGLVSLILVV 540
           VRVAG+LYDREA RQMEAAG RR  GEDL C+GVECYR AFLIITAATV G  VSLILVV
Sbjct: 481 VRVAGHLYDREATRQMEAAGRRRNIGEDLNCLGVECYRKAFLIITAATVLGAFVSLILVV 540

Query: 541 RTWKFYKGDIYSKFREEEDGATEGSKMAAPNSTMPAAKT 573
           RTWKFYKGDIY KFREE     E    A  NS +  ++T
Sbjct: 541 RTWKFYKGDIYRKFREEAGEEVEVQMAAPTNSAVAKSQT 578

BLAST of MS019624 vs. ExPASy TrEMBL
Match: A0A5A7TKH4 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002830 PE=4 SV=1)

HSP 1 Score: 935.3 bits (2416), Expect = 1.3e-268
Identity = 485/580 (83.62%), Postives = 522/580 (90.00%), Query Frame = 0

Query: 1   MAASSF--SSGNAFWYQLLFGRWFSVFASVLIMSVSGATYMFGLYSSDIKSSLAYDQTTL 60
           MAAS+   +S N FWYQ LFGRWFSVFAS+LIMSV+GATYMF LYSSDIKSSL YDQTTL
Sbjct: 1   MAASTSNPTSANPFWYQFLFGRWFSVFASILIMSVAGATYMFALYSSDIKSSLGYDQTTL 60

Query: 61  NLLSFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWLAVTARIPNPQIWQM 120
           NLLSFFKDLGGNVGVI GLINEVAP WVVLLIGAVMNLFGYTMIWLAVT RIP PQIW M
Sbjct: 61  NLLSFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHM 120

Query: 121 CLYICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAVLTQLYHAFYGNNS 180
           CLYIC+GANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGA+L+QL+HAFYGN+S
Sbjct: 121 CLYICLGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNDS 180

Query: 181 KSLILLIAWLPAAVSVAFLRFVRIIKDLRQPNELRVFYHFLYISLGLAGSLMVLIIGQNR 240
           KSLILLI WLPAAVSV FLRFVRIIKDLRQPNE+ VFYH LYISL LAG+LMVLII Q+ 
Sbjct: 181 KSLILLIGWLPAAVSVVFLRFVRIIKDLRQPNEVNVFYHILYISLSLAGTLMVLIILQSL 240

Query: 241 LRFQQMEYAGSACIVLVLLLLPLAVVFREELSVWKSKIENPISQLELASQQSPP-----L 300
           LRFQQ++Y GSA +V+VLLLLPLA+VFREELSVWKSKI NPI QLELASQQ PP     +
Sbjct: 241 LRFQQIQYVGSAIVVIVLLLLPLAIVFREELSVWKSKIANPILQLELASQQPPPPLTSTV 300

Query: 301 P-PPSSPQSDSCVQNVFKPPNRGEDYTILQAVFSVDMMILFVATICGVGGTLTAIDNLGQ 360
           P  PSSP SDSC +N+FKPPNRGEDYTI QA+FS+DM+ILF+ATICGVGGTLTAIDNLGQ
Sbjct: 301 PLAPSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQ 360

Query: 361 IGESLGYPSQSTTTFISLVSIWNYLGRVVAGFASEFLWKKYKIPRPLFFFITLLLSCVGH 420
           IGESL YPS STTTFISLVSIWNYLGRVV+GF SE+ WKKYK+PRPLF F+TL+LSC+GH
Sbjct: 361 IGESLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGH 420

Query: 421 LLIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYIL 480
           LLIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYN+GG ASPIGAYI+
Sbjct: 421 LLIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIM 480

Query: 481 NVRVAGNLYDREARRQMEAAGLRRKTGEDLRCVGVECYRMAFLIITAATVFGGLVSLILV 540
           NVRVAG+LYDREA+RQMEAAG  RK GEDL C+GVECYR AFLIITAATVFG LVSLILV
Sbjct: 481 NVRVAGHLYDREAQRQMEAAGRLRKKGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 540

Query: 541 VRTWKFYKGDIYSKFREEEDGATEGSKMAAP-NSTMPAAK 572
           VRTWKFYKGDIY +FR EE G     KMAAP NST+  AK
Sbjct: 541 VRTWKFYKGDIYRRFRAEE-GEDIEMKMAAPTNSTLTTAK 579

BLAST of MS019624 vs. ExPASy TrEMBL
Match: A0A5A7TKY5 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002840 PE=4 SV=1)

HSP 1 Score: 921.8 bits (2381), Expect = 1.4e-264
Identity = 468/570 (82.11%), Postives = 512/570 (89.82%), Query Frame = 0

Query: 7   SSGNAFWYQLLFGRWFSVFASVLIMSVSGATYMFGLYSSDIKSSLAYDQTTLNLLSFFKD 66
           +SGN FWY LLFGRWFSVFAS+LIMSVSGATYMFGLYSS IKSSLAYDQTTLNLLSFFKD
Sbjct: 3   TSGNPFWYHLLFGRWFSVFASILIMSVSGATYMFGLYSSHIKSSLAYDQTTLNLLSFFKD 62

Query: 67  LGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWLAVTARIPNPQIWQMCLYICIGA 126
           LG N+GVI GL+NEVAP WVVLLIG VMNLFGYTMIWLAVT RIPNPQIW MCLYICIGA
Sbjct: 63  LGANIGVISGLVNEVAPAWVVLLIGVVMNLFGYTMIWLAVTNRIPNPQIWHMCLYICIGA 122

Query: 127 NSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAVLTQLYHAFYGNNSKSLILLIA 186
           NSQTFANT AL+TCVKNFPESRGS+LGL KGFVGLSGA+L+QL+HAFYGNNSKSLI LIA
Sbjct: 123 NSQTFANTAALITCVKNFPESRGSILGLFKGFVGLSGAILSQLFHAFYGNNSKSLIFLIA 182

Query: 187 WLPAAVSVAFLRFVRIIKDLRQPNELRVFYHFLYISLGLAGSLMVLIIGQNRLRFQQMEY 246
           WLP+AVSV   RFVRIIKDLRQPNEL+VFYH LYISLGLAGSLMV II QNRLRFQQ+ Y
Sbjct: 183 WLPSAVSVILHRFVRIIKDLRQPNELKVFYHVLYISLGLAGSLMVCIILQNRLRFQQIHY 242

Query: 247 AGSACIVLVLLLLPLAVVFREELSVWKSKIENPISQLELASQQSPPLPPPSSPQSDSCVQ 306
            GSA +V+VLLLLPLA+VFREEL +W+SKI+NPI QLELASQ  P  PPP +P SDSC +
Sbjct: 243 VGSAIVVIVLLLLPLAIVFREELRIWRSKIKNPIPQLELASQLPP--PPPPNPPSDSCFK 302

Query: 307 NVFKPPNRGEDYTILQAVFSVDMMILFVATICGVGGTLTAIDNLGQIGESLGYPSQSTTT 366
           N+F PPNRGEDYTI QA+FS+DM+ILF+ATICGVGGTLTAIDNLGQIGESL YPS STTT
Sbjct: 303 NMFNPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQIGESLRYPSHSTTT 362

Query: 367 FISLVSIWNYLGRVVAGFASEFLWKKYKIPRPLFFFITLLLSCVGHLLIAFGVPNSLYFS 426
           FISLVSIWNYLGRVV+GF SE+ WKKYK+PRPLF F+TL+LSC+GHLLIAFGVPNSLYFS
Sbjct: 363 FISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGHLLIAFGVPNSLYFS 422

Query: 427 SIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNVRVAGNLYDREAR 486
           SIIIGFCFGAQWPLIFAIISEIFGLKYYATLYN+GG ASPIGAYI+NVRVAG+LYD EA 
Sbjct: 423 SIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIMNVRVAGHLYDMEAE 482

Query: 487 RQMEAAGLRRKTGEDLRCVGVECYRMAFLIITAATVFGGLVSLILVVRTWKFYKGDIYSK 546
           RQMEAAG  RK GEDL C+GVECY+ AFLIIT +TV GGLVSLILVVRTWKFYK DIY +
Sbjct: 483 RQMEAAGRLRKEGEDLSCLGVECYKKAFLIITGSTVLGGLVSLILVVRTWKFYKDDIYRR 542

Query: 547 FREEEDGATEGSKMAAP-NSTMPAAKTESA 576
           F+E+E G     K+AAP N+++ AAKT SA
Sbjct: 543 FKEKE-GEDIEIKIAAPTNNSLTAAKTVSA 569

BLAST of MS019624 vs. ExPASy TrEMBL
Match: A0A0A0LEA4 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G742600 PE=4 SV=1)

HSP 1 Score: 904.0 bits (2335), Expect = 3.1e-259
Identity = 473/580 (81.55%), Postives = 510/580 (87.93%), Query Frame = 0

Query: 1   MAASSF--SSGNAFWYQLLFGRWFSVFASVLIMSVSGATYMFGLYSSDIKSSLAYDQTTL 60
           MAASS   +S N FWYQ LFGRWFSV AS+LIMSV+GATYMF LYSSDIKSSL YDQTTL
Sbjct: 1   MAASSSNPTSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTL 60

Query: 61  NLLSFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWLAVTARIPNPQIWQM 120
           NLLSFFKDLGGNVGVI GL NEVAP WVVLLIGAVMNLFGYTMIWLAVT RIP PQIW M
Sbjct: 61  NLLSFFKDLGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHM 120

Query: 121 CLYICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAVLTQLYHAFYGNNS 180
           CLYI IGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGA+L+QLY AFYGNN 
Sbjct: 121 CLYITIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNP 180

Query: 181 KSLILLIAWLPAAVSVAFLRFVRIIKDLRQPNELRVFYHFLYISLGLAGSLMVLIIGQNR 240
           +SLILLIAWLPAAVSV  LRFVRIIKDLRQPNEL+VFYHFLYISLGLAG+LMVLII Q+ 
Sbjct: 181 ESLILLIAWLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSL 240

Query: 241 LRFQQMEYAGSACIVLVLLLLPLAVVFREELSVWKSKIENPISQLELASQQSPP------ 300
           LRFQQ++Y GSA +V+VLLLLPL +VFREELSVWKSKI +P+ QLE ASQQ PP      
Sbjct: 241 LRFQQIQYVGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTV 300

Query: 301 LPPPSSPQSDSCVQNVFKPPNRGEDYTILQAVFSVDMMILFVATICGVGGTLTAIDNLGQ 360
              PSSP S+SC +N+F PP+RGEDYTI QA+FS+D++ILF+ATICGVGGTLTAIDNLGQ
Sbjct: 301 SLAPSSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQ 360

Query: 361 IGESLGYPSQSTTTFISLVSIWNYLGRVVAGFASEFLWKKYKIPRPLFFFITLLLSCVGH 420
           IGESLGY S S TTFISLVSIWNYLGRVV+GF SE+ WKKYK+PRPLF F TL+LSCVGH
Sbjct: 361 IGESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGH 420

Query: 421 LLIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYIL 480
           LLIAFGVPNSLYFSSI+IGFCFGAQWPLI+AIISEIFGLKYYATL ++ G ASPIGAYIL
Sbjct: 421 LLIAFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYIL 480

Query: 481 NVRVAGNLYDREARRQMEAAGLRRKTGEDLRCVGVECYRMAFLIITAATVFGGLVSLILV 540
           NVRVAG+LYDREA+RQMEA G RR  GEDL C+GVECYR AFLIITAATVFG LVSLILV
Sbjct: 481 NVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 540

Query: 541 VRTWKFYKGDIYSKFREEEDGATEGSKMAAP-NSTMPAAK 572
           VRTWKFYKGDIY KFRE E G     KMAAP N T+ AAK
Sbjct: 541 VRTWKFYKGDIYRKFREVE-GEDIEMKMAAPTNGTLTAAK 579

BLAST of MS019624 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 709.5 bits (1830), Expect = 2.2e-204
Identity = 370/579 (63.90%), Postives = 439/579 (75.82%), Query Frame = 0

Query: 1   MAASSFSSGNAFWYQLLFGRWFSVFASVLIMSVSGATYMFGLYSSDIKSSLAYDQTTLNL 60
           +AAS   S  +   Q+L GRWF  F S+LIMS +GATYMFG+YS DIK +L YDQTTLNL
Sbjct: 2   VAASPGGSMKSLTIQILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNL 61

Query: 61  LSFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWLAVTARIPNPQIWQMCL 120
           LSFFKDLG NVGV+ GL+NEV PPW +LLIGA++N FGY MIWLAVT RI  PQ+W MCL
Sbjct: 62  LSFFKDLGANVGVLAGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCL 121

Query: 121 YICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAVLTQLYHAFYGNNSKS 180
           YIC+GANSQ+FANTG+LVTCVKNFPESRG VLG+LKG+VGLSGA++TQLY AFYG ++K 
Sbjct: 122 YICVGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKE 181

Query: 181 LILLIAWLPAAVSVAFLRFVRIIKDLRQPNELRVFYHFLYISLGLAGSLMVLIIGQNRLR 240
           LIL+I WLPA VS AFLR +RI+K  RQ NEL+VFY+FLYISLGLA  LMV+II      
Sbjct: 182 LILMIGWLPAIVSFAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSG 241

Query: 241 FQQMEYAGSACIVLVLLLLPLAVVFREELSVWKSK--IENPISQLELASQQSPPLPPPSS 300
           F Q E+ GSA +V+VLLLLP+ VV  EE  +WK K    N  + + + +++ P L     
Sbjct: 242 FTQSEFGGSAAVVIVLLLLPIIVVILEEKKLWKEKQVALNDPAPINVVTEK-PKLDSSEF 301

Query: 301 PQSD--------------SCVQNVFKPPNRGEDYTILQAVFSVDMMILFVATICGVGGTL 360
              D              SC   VF PP RG+DYTILQA+FSVDM+ILF+ATICGVGGTL
Sbjct: 302 KDDDGEESKEVVEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTL 361

Query: 361 TAIDNLGQIGESLGYPSQSTTTFISLVSIWNYLGRVVAGFASEFLWKKYKIPRPLFFFIT 420
           TAIDNLGQIG SLGYP +S +TF+SLVSIWNY GRVV+G  SE    KYK PRPL   + 
Sbjct: 362 TAIDNLGQIGNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMV 421

Query: 421 LLLSCVGHLLIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVA 480
           LLLSC GHLLIAF VP  LY +S+IIGFCFGAQWPL+FAIISEIFGLKYY+TLYN G VA
Sbjct: 422 LLLSCAGHLLIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVA 481

Query: 481 SPIGAYILNVRVAGNLYDREARRQMEAAGLRRKTGEDLRCVGVECYRMAFLIITAATVFG 540
           SPIG+Y+LNVRVAG LYD EA +Q +A G  R  G+DL C+G  C++++F+II A T+FG
Sbjct: 482 SPIGSYLLNVRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFG 541

Query: 541 GLVSLILVVRTWKFYKGDIYSKFREEEDGATEGSKMAAP 564
            LVS++LV+RT KFYK DIY KFRE+   A    +MAAP
Sbjct: 542 VLVSMVLVIRTKKFYKSDIYKKFREKALAAE--MEMAAP 577

BLAST of MS019624 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 661.8 bits (1706), Expect = 5.1e-190
Identity = 340/549 (61.93%), Postives = 412/549 (75.05%), Query Frame = 0

Query: 19  GRWFSVFASVLIMSVSGATYMFGLYSSDIKSSLAYDQTTLNLLSFFKDLGGNVGVIGGLI 78
           GRWF VFAS LIM+ +GATY+FG YS DIKS+L YDQTTLNLL FFKDLG NVGV+ GLI
Sbjct: 16  GRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGANVGVLSGLI 75

Query: 79  NEVAPPWVVLLIGAVMNLFGYTMIWLAVTARIPNPQIWQMCLYICIGANSQTFANTGALV 138
            EV P W VL IG+ MN  GY MIWL VT ++  P++WQMCLYICIGANSQ FANTGALV
Sbjct: 76  AEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNFANTGALV 135

Query: 139 TCVKNFPESRGSVLGLLKGFVGLSGAVLTQLYHAFYGNNSKSLILLIAWLPAAVSVAFLR 198
           TCVKNFPESRG +LGLLKG+VGLSGA+ TQLY A YG++SKSLILLIAWLPAAVS+ F+ 
Sbjct: 136 TCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLIAWLPAAVSLVFVY 195

Query: 199 FVRIIKDLRQPNELRVFYHFLYISLGLAGSLMVLIIGQNRLRFQQMEYAGSACIVLVLLL 258
            +R  K +RQ NEL VFY FLYIS+ LA  LM + I + ++ F +  YA SA I   LL 
Sbjct: 196 LIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAASATICCALLF 255

Query: 259 LPLAVVFREELSVW-----------KSKIENPISQLELASQQSPPLPPPSSPQSDSCVQN 318
           +PL V  ++EL VW           + K+E P  +L+L  Q           ++ SC   
Sbjct: 256 VPLTVSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDL-DQDKAAKVNGEEKETKSCFST 315

Query: 319 VFKPPNRGEDYTILQAVFSVDMMILFVATICGVGGTLTAIDNLGQIGESLGYPSQSTTTF 378
           VF PP RGEDYTILQA+ S DM+ILFVAT CG+G +LTA+DNLGQIGESLGYP+ + ++F
Sbjct: 316 VFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGESLGYPNHTVSSF 375

Query: 379 ISLVSIWNYLGRVVAGFASEFLWKKYKIPRPLFFFITLLLSCVGHLLIAFGVPNSLYFSS 438
           +SLVSIWNY GRV +GF SE+L  KYK+PRPL   + LLLSC GHLLIAF VP S+Y +S
Sbjct: 376 VSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIAFPVPGSVYIAS 435

Query: 439 IIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNVRVAGNLYDREARR 498
           I++GF FGAQ PL+FAIISE+FGLKYY+TL+N G +ASP+G+YILNVRV G LYD+EA +
Sbjct: 436 ILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRVTGMLYDKEALK 495

Query: 499 QMEAAGLRRKTGEDLRCVGVECYRMAFLIITAATVFGGLVSLILVVRTWKFYKGDIYSKF 557
           Q+ A GL RK  +DL C+G +CY++ FLI+ A T FG LVSL L +RT +FYKGDIY KF
Sbjct: 496 QLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIRTREFYKGDIYKKF 555

BLAST of MS019624 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 336.7 bits (862), Expect = 3.8e-92
Identity = 204/540 (37.78%), Postives = 312/540 (57.78%), Query Frame = 0

Query: 15  QLLFGRWFSVFASVLIMSVSGATYMFGLYSSDIKSSLAYDQTTLNLLSFFKDLGGNVGVI 74
           ++L  +W ++ AS+ I   +G +Y FG+YS+ +KS+ +YDQ+TL+ +S FKD+GGNVGV+
Sbjct: 4   EILRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNVGVL 63

Query: 75  GGLINEVAP--------------PWVVLLIGAVMNLFGYTMIWLAVTARIPNPQIWQMCL 134
            GL+   A               PWVV+LIGA++N  GY ++W +VT  I  P +  MCL
Sbjct: 64  SGLVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPVMCL 123

Query: 135 YICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAVLTQLYHAFYGNNSKS 194
           ++ I A S TF NT  +V+ ++NF +  G+ +G++KGFVGLSGA+L QLY      + K+
Sbjct: 124 FMFIAAQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGDPKT 183

Query: 195 LILLIAWLPAAVSVAFLRFVRIIKDLRQPNELRVFYHFLYISLGLAGSLMVLIIGQNRLR 254
            ILL+A +P+ +SV  +  VR+ K     +E +       +SL +A  LM+ II ++ L 
Sbjct: 184 FILLLAIVPSLLSVLVMPLVRVYK-TSTVDEKKHLDGLSTLSLIIAAYLMITIILKSTLS 243

Query: 255 FQQMEYAGSACIVLVLLLLPLAVVFREELSVWKSKIENPISQLELASQQSPPLPPPSSPQ 314
                 A +  ++LVLL  PL V  R      +  IE P+S     S  SP +    +  
Sbjct: 244 LPSWANAVTLAVLLVLLSSPLLVAVRAH----RDSIEKPLS-----SVYSPLVDNLEATT 303

Query: 315 SDSCVQNVFKPPNRGEDYTILQAVFSVDMMILFVATICGVGGTLTAIDNLGQIGESLGYP 374
           S   +       +  +   +LQA+ +VD  +LF+A ICG+G  ++ I+N+ QIGESL Y 
Sbjct: 304 SGEILM-----LDEDKSLNLLQAMCNVDFWLLFLAMICGMGSGISTINNIRQIGESLRYT 363

Query: 375 SQSTTTFISLVSIWNYLGRVVAGFASEFLWKKYKIPRPLFFFITLLLSCVGHLLIAFGVP 434
           S    + ++L +IWN++GR   G+ S++L  +   PRPL    TL    +GHL+IA G  
Sbjct: 364 SVEINSLLALWNIWNFIGRFGGGYVSDWLLHRKGWPRPLLMATTLGTMTIGHLIIASGFQ 423

Query: 435 NSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNVRVAGNL 494
            +LY  SII+G C+G+QW L+  I SE+FG+K+  T+YN   +ASP+G+YI +VR+ G +
Sbjct: 424 GNLYPGSIIVGICYGSQWSLMPTITSELFGVKHMGTIYNTISIASPMGSYIFSVRLIGYI 483

Query: 495 YDREARRQMEAAGLRRKTGEDLRCVGVECYRMAFLIITAATVFGGLVSLILVVRTWKFYK 541
           YDR               GE   C G  C+R+A+++I +    G LVS +LV RT   Y+
Sbjct: 484 YDRTI------------IGEGNTCYGPHCFRLAYVVIASVAFLGFLVSCVLVFRTKTIYR 516

BLAST of MS019624 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 335.9 bits (860), Expect = 6.5e-92
Identity = 205/547 (37.48%), Postives = 307/547 (56.12%), Query Frame = 0

Query: 15  QLLFGRWFSVFASVLIMSVSGATYMFGLYSSDIKSSLAYDQTTLNLLSFFKDLGGNVGVI 74
           ++L  +W ++ AS+ I   SGA+Y FG+YS+ +KS+ +YDQ+TL+ +S FKD+G N GV 
Sbjct: 2   EILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVF 61

Query: 75  GGLINEVAP----------------PWVVLLIGAVMNLFGYTMIWLAVTARIPNPQIWQM 134
            GL+   A                 PWVVL +GA+    GY +IW +VT  I  P +  M
Sbjct: 62  SGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLM 121

Query: 135 CLYICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAVLTQLYHAFYGNNS 194
           CL++ + A SQTF NT  +V+ V+NF +  G+ +G++KGF+GLSGA+L QLY      + 
Sbjct: 122 CLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGDP 181

Query: 195 KSLILLIAWLPAAVSVAFLRFVRIIK-----DLRQPNELRVFYHFLYISLGLAGSLMVLI 254
            S ILL+A  P  +S+  +  VRI +     D +  N L        +SL +A  LM++I
Sbjct: 182 ASFILLLAVTPTVLSLLVMPLVRIYETSVADDKKHLNGLSA------VSLIIAAYLMIII 241

Query: 255 IGQNRLRFQQMEYAGSACIVLVLLLLPLAVVFREELSVWKSKIENPISQLELASQQSPPL 314
           I +N           +   +LV+L LPL +  R +    +  + +  S   L S      
Sbjct: 242 ILKNTFGLSSWANIVTLVCLLVMLALPLLIARRAQRDGMEKTVPHDYS--PLISSPKATT 301

Query: 315 PPPSSPQSDSCVQNVFKPPNRGEDYTILQAVFSVDMMILFVATICGVGGTLTAIDNLGQI 374
               S + DS V+         E+  +LQA+  +   +LF+A ICG+G  L+ I+N+ QI
Sbjct: 302 SGNQSSEGDSKVE-----AGLSENLNLLQAMKKLSFWLLFLAMICGMGSGLSTINNIRQI 361

Query: 375 GESLGYPSQSTTTFISLVSIWNYLGRVVAGFASEFLWKKYKIPRPLFFFITLLLSCVGHL 434
           GESL Y S    + +SL SIWN+LGR  AG+AS+ L  K   PRPL    TL    +GHL
Sbjct: 362 GESLRYSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATLGTMSIGHL 421

Query: 435 LIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILN 494
           +IA G   +LY  S+I+G C+G+QW L+  I SE+FG+++  T++N   VASPIG+YI +
Sbjct: 422 IIASGFQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVASPIGSYIFS 481

Query: 495 VRVAGNLYDREARRQMEAAGLRRKTGEDLRCVGVECYRMAFLIITAATVFGGLVSLILVV 541
           VR+ G +YD+ A            +GE   C G  C+R++F+I+ +   FG LV+++L  
Sbjct: 482 VRLIGYIYDKTA------------SGEGNTCYGSHCFRLSFIIMASVAFFGFLVAIVLFF 523

BLAST of MS019624 vs. TAIR 10
Match: AT2G34355.1 (Major facilitator superfamily protein )

HSP 1 Score: 334.7 bits (857), Expect = 1.4e-91
Identity = 208/541 (38.45%), Postives = 304/541 (56.19%), Query Frame = 0

Query: 20  RWFSVFASVLIMSVSGATYMFGLYSSDIKSSLAYDQTTLNLLSFFKDLGGNVGVIGGLI- 79
           +W +  AS+ I S SGATY F +YSS +KSS +YDQ+TL+ +S FKD+GG  G+I G + 
Sbjct: 7   KWVAAAASIWIQSFSGATYTFAIYSSILKSSQSYDQSTLDFVSVFKDIGGTFGIISGFLY 66

Query: 80  -------NEVAPPWVVLLIGAVMNLFGYTMIWLAVTARIPNPQIWQMCLYICIGANSQTF 139
                       PWVV+ +G V    G+  IW +V   I  P +  MCL++ +  +S  F
Sbjct: 67  TAMTSKSRGFGGPWVVVFVGLVQWFVGFFFIWASVVGLIAPPPVPLMCLFVFLAGHSLPF 126

Query: 140 ANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAVLTQLYHAFYG--NNSKSLILLIAWLP 199
            NT  +VT  +NF +  G+ +G+++GF+GLSGA+L QLYHA  G   N  + ILL+A +P
Sbjct: 127 FNTANVVTAARNFSQYGGTAVGIMQGFLGLSGAILIQLYHAVCGGEGNPATFILLLAIVP 186

Query: 200 AAVSVAFLRFVRI-----IKDLRQPNELRVFYHFLYISLGLAGSLMVLIIGQNRLRFQQM 259
             V    + FVR+     I D +  + L        IS+ +A  LMV+I  +N L   + 
Sbjct: 187 TLVMFLAMPFVRVYETVTISDKKHLDGLSA------ISMIIAAYLMVVITVENVLGLSRS 246

Query: 260 EYAGSACIVLVLLLLPLAVVFR--EELSVWKSKIENPISQLELASQQSPPLPPPSS---P 319
               S  +VL+LL  PL V  R   E     S ++ P+         S  L PPSS   P
Sbjct: 247 MQIFSFILVLLLLASPLLVAVRALREKRQTLSSLDGPV------LDTSALLDPPSSNIFP 306

Query: 320 QSDSCVQNVFKPPNRGEDYTILQAVFSVDMMILFVATICGVGGTLTAIDNLGQIGESLGY 379
             D  V          ED  IL+A+ +V+  +LF+A +CG+G     ++N+ QIGESL Y
Sbjct: 307 DGDHLV---------AEDSNILEAMSTVNFWLLFLAMLCGMGSGFATVNNMRQIGESLRY 366

Query: 380 PSQSTTTFISLVSIWNYLGRVVAGFASEFLWKKYKIPRPLFFFITLLLSCVGHLLIAFGV 439
            S    + +SL SIWN+LGR  AG+ S+    K+  PRP+F  ITL +  +GH+++A GV
Sbjct: 367 SSVQLNSLVSLWSIWNFLGRFGAGYVSDTFLHKHSWPRPIFMAITLGVMAIGHIIVASGV 426

Query: 440 PNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNVRVAGN 499
             SLY  S++IG  +G+QW L+  I SEIFG+++  T+Y    +A PIG+YIL+V+V G 
Sbjct: 427 QGSLYAGSVLIGMAYGSQWSLMPTITSEIFGIRHMGTIYFTISIAGPIGSYILSVKVIGY 486

Query: 500 LYDREARRQMEAAGLRRKTGEDLRCVGVECYRMAFLIITAATVFGGLVSLILVVRTWKFY 541
            YD+ A            + +D  C G +C+R +F+I+ +  +FG LV+ +L  RT KFY
Sbjct: 487 FYDKVA------------SEDDNSCFGSQCFRTSFMIMASVALFGSLVASVLFFRTHKFY 514