MS019621 (gene) Bitter gourd (TR) v1

Overview
NameMS019621
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionCarbonic anhydrase
Locationscaffold729: 1060301 .. 1063143 (-)
RNA-Seq ExpressionMS019621
SyntenyMS019621
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGGACGAAAGTGAGTTTGATTACATAAGAGGAAGCAAGAAAGGGCCTTCAAAATGGGGAGAGATCAAGAGAGAATGGGAGAGGTGCAAAAATGGCGAAATGCAATCTCCCATTGATTTGTCCTCCACAAGAGTCAAAGCTATCAAAACTTTGGGTCACCTCAACAACAAATACAAACCCTCCAATGCTATTCTCAAGAATCGTGGCCATGATATTTCGGTAACACATTATACAAAATTTTGCTTTAATTATTCAAACATTGGTAGTTATTTGGTTGCTTGTACTCTCACTTTTGTTCTATTTTAATGAGTTAATTTACTTTCAAAGTGTCTATCTTGATTCTAACATTTATTCATTTTAATTCTCTATATTTTCAAAACGTTTTGGACTTTTTAATTTTTAAAATGACGCTAACATTTATTCATTTTAATTCTCTATATTTTCAAAACGTTTTGGACTTTTTAATTTTTAAAATTCGTCATTTTAATCTCTATTTATAAAATTTTAACACAAACTTTATACACCATGGATAAATTCTTTAATACAAAATTTATAACTATATTTCAAGAAATTTAATGAGTGATCAGTGTCATATGCTAAAATTCTAGAAAGAGAAAGAATGGATCAAAATTGTGACTTTTTAATGGTGTATAGACCAAAATGAAAGTTTTGAAATTAGTGAATAAACTCAACAAGATTTTAAAGGATAGTGACAAAGAAAAAAAGAAAAAGACATCCTTTCGAAAATTTAAGAACCAAAATGAAGTAAAGTAGTGAGTGTGTATGAGGATCAAAATAATATTTAAACACATGAAAATTTAACAAAATTGGTAGTGTTGGAGTCCATCATAATCGTCACTATCTCTACTATGGTGAAAAGTTAGGTAAGTTAGATAGTTGAGTTATATACTCATCTGAGCATTTTAGATAAAATATATAATTTATATGACAAAATAGAAAAAAATTAATTTCTTACTTGACCAAGCTCAAAAGGAAAGAATAGGTAATCTCAAATGAATATATACTTTAAATTAAATTATTATAAGAGAAAATACCTGCTCTGCTGCTATGGTATTTATCGTCTTAAAATTCTATGGTTGGACAGATTAAGTGGGAGGGAAATGCAGGATCAATCAAGATTAATGACACAAATTATCTTCTTCAACAAATCCATTGGCACTCTCCTTCTGAACATACTCTTAACGGGAGGAGGTTCAATCTCTTCTCTCTAACTTCCTTTTGAATATTTTTAATTTTTATACTTGTAATTGACAAATTTTTCATCTGATAAATCTACTCATGAAAAACAAAATCATTAGTGAATGAGACTTGAAGTTATATTATGATCACTTTCAAACTAATAACAAGGATTATTTTTCGTTGATTTAGGTACGATTTAGAGCTTCATTTAGTTCACCAAAGTTCACATTCAGATGTCAAATATCCCATCGCCGTTGTTGGCATCTTCTACAAGATGGGTCGTCCCGACCCTTTTCTCTCCAAGGTAAATTTCAACATGGATAAAATTTATCGATCTTAATTGATTAATTTTTCTCAATCTAATATATGATACATCAAGGCTAACAAACTATTAAAATGTGGCAGCTGAGTAGGAAAATAAAGGCATTGGGTGAAGAAAAGGAGAAAAAATTGGGAAGAATAGATCCTAGAATGATAAAAATGGGTAGCATCAAGTATTATAGATACATGGGCTCTCTCACTGTTCCTCCTTGCACTGAAGGTGTCATTTGGAACATTAACCAAAAGGTATGCTTCTACTACTTCCTTAATTTCTCCCTTGATGTTAAATCAACATGAGAAAATAAATTAAAATGCAATTATGATTTTCTTGAGATTAAACTAATTTTATAATTTATTCAAACATGCTACGTACCAAAGTCTCCCATGTCGAGGTTTGTGCTTAGTTTAGATATTTCCTTAGGTTAAAAGCATCTCACTGAGAAAAAATAAAAGATTTAACAAATGTTTATAATATGAGATGAAGTCTCCATGAATATTGATATGCGTTAAGGAAGAATAAACCACCACTCAACCTAAAAGTAAGTTGATAGGTTGGATAAATTTAATTTTAGATTGTCAACGCTCTTCGATAATTGCACAAGGCCCAACAAGTGAAAATCACTATAAATTGGGAGGAAACAAACATATGACCAGATATTAAAATGAATAAATCACCACTCAACCCAAAATCTTAACATGGTATAAGAGTAGGAGATCCTGTATTTGAATCTCTGCATTGTTGTTTCCTCCTCATTTATAGTGATTCCCACTCGTTGGGCTTTGTGCAATTTTCCAAGCCATGGGAGAAATTTACCAAACCCATCAGTGTAAGGTTGAGTGGTGGTTGAGCATTCCGTTGAAAAATGAAAGAATCTTACGTTGGAGAGATAAAGAACATAACAAAAGTTTATAATATACATCGGTTATACTCCGACTTTCATTGTTGATTGATTTTGATATGGAATCATATGAATATTAATATCTTATAATAAAAGTAATAATATATTTGAGCATAGCTGAATTGGGTGAGACGTATTTAATTTTATATTTTTTATTTATTTGTATGGTTTGATATCAGATAGGAACAGTCTCACGGGAACAGGTGAAGCTACTGAGATCGGCTGTTCACGACGTAAGTTCTATAGAATGTTTCTATTTCCAATTTTAATTAAAACGACAGGTCGGCTTTTATTATTTTTCTTATTATTACTTTTTTTTTTTAATGTGAATTGCAGTCTGCAGAGAAGAATGCAAGACCAATCCAACCACATAATGGACGACAAGTCGACCTCTACGACCCAAACCCAACACACACACCCAAT

mRNA sequence

GAGGACGAAAGTGAGTTTGATTACATAAGAGGAAGCAAGAAAGGGCCTTCAAAATGGGGAGAGATCAAGAGAGAATGGGAGAGGTGCAAAAATGGCGAAATGCAATCTCCCATTGATTTGTCCTCCACAAGAGTCAAAGCTATCAAAACTTTGGGTCACCTCAACAACAAATACAAACCCTCCAATGCTATTCTCAAGAATCGTGGCCATGATATTTCGATTAAGTGGGAGGGAAATGCAGGATCAATCAAGATTAATGACACAAATTATCTTCTTCAACAAATCCATTGGCACTCTCCTTCTGAACATACTCTTAACGGGAGGAGGTACGATTTAGAGCTTCATTTAGTTCACCAAAGTTCACATTCAGATGTCAAATATCCCATCGCCGTTGTTGGCATCTTCTACAAGATGGGTCGTCCCGACCCTTTTCTCTCCAAGCTGAGTAGGAAAATAAAGGCATTGGGTGAAGAAAAGGAGAAAAAATTGGGAAGAATAGATCCTAGAATGATAAAAATGGGTAGCATCAAGTATTATAGATACATGGGCTCTCTCACTGTTCCTCCTTGCACTGAAGGTGTCATTTGGAACATTAACCAAAAGATAGGAACAGTCTCACGGGAACAGGTGAAGCTACTGAGATCGGCTGTTCACGACTCTGCAGAGAAGAATGCAAGACCAATCCAACCACATAATGGACGACAAGTCGACCTCTACGACCCAAACCCAACACACACACCCAAT

Coding sequence (CDS)

GAGGACGAAAGTGAGTTTGATTACATAAGAGGAAGCAAGAAAGGGCCTTCAAAATGGGGAGAGATCAAGAGAGAATGGGAGAGGTGCAAAAATGGCGAAATGCAATCTCCCATTGATTTGTCCTCCACAAGAGTCAAAGCTATCAAAACTTTGGGTCACCTCAACAACAAATACAAACCCTCCAATGCTATTCTCAAGAATCGTGGCCATGATATTTCGATTAAGTGGGAGGGAAATGCAGGATCAATCAAGATTAATGACACAAATTATCTTCTTCAACAAATCCATTGGCACTCTCCTTCTGAACATACTCTTAACGGGAGGAGGTACGATTTAGAGCTTCATTTAGTTCACCAAAGTTCACATTCAGATGTCAAATATCCCATCGCCGTTGTTGGCATCTTCTACAAGATGGGTCGTCCCGACCCTTTTCTCTCCAAGCTGAGTAGGAAAATAAAGGCATTGGGTGAAGAAAAGGAGAAAAAATTGGGAAGAATAGATCCTAGAATGATAAAAATGGGTAGCATCAAGTATTATAGATACATGGGCTCTCTCACTGTTCCTCCTTGCACTGAAGGTGTCATTTGGAACATTAACCAAAAGATAGGAACAGTCTCACGGGAACAGGTGAAGCTACTGAGATCGGCTGTTCACGACTCTGCAGAGAAGAATGCAAGACCAATCCAACCACATAATGGACGACAAGTCGACCTCTACGACCCAAACCCAACACACACACCCAAT

Protein sequence

EDESEFDYIRGSKKGPSKWGEIKREWERCKNGEMQSPIDLSSTRVKAIKTLGHLNNKYKPSNAILKNRGHDISIKWEGNAGSIKINDTNYLLQQIHWHSPSEHTLNGRRYDLELHLVHQSSHSDVKYPIAVVGIFYKMGRPDPFLSKLSRKIKALGEEKEKKLGRIDPRMIKMGSIKYYRYMGSLTVPPCTEGVIWNINQKIGTVSREQVKLLRSAVHDSAEKNARPIQPHNGRQVDLYDPNPTHTPN
Homology
BLAST of MS019621 vs. NCBI nr
Match: XP_022144728.1 (alpha carbonic anhydrase 7-like [Momordica charantia])

HSP 1 Score: 508.8 bits (1309), Expect = 2.6e-140
Identity = 246/248 (99.19%), Postives = 247/248 (99.60%), Query Frame = 0

Query: 1   EDESEFDYIRGSKKGPSKWGEIKREWERCKNGEMQSPIDLSSTRVKAIKTLGHLNNKYKP 60
           EDESEFDYIRGSKKGPSKWGEIKREWERCKNGEMQSPIDLSSTRVKAIKTLGHLNNKYKP
Sbjct: 29  EDESEFDYIRGSKKGPSKWGEIKREWERCKNGEMQSPIDLSSTRVKAIKTLGHLNNKYKP 88

Query: 61  SNAILKNRGHDISIKWEGNAGSIKINDTNYLLQQIHWHSPSEHTLNGRRYDLELHLVHQS 120
           SNAILKNRGHDISIKWEGNAGSIKINDTNYLLQQIHWHSPSEHTLNGRRYDLELHLVHQS
Sbjct: 89  SNAILKNRGHDISIKWEGNAGSIKINDTNYLLQQIHWHSPSEHTLNGRRYDLELHLVHQS 148

Query: 121 SHSDVKYPIAVVGIFYKMGRPDPFLSKLSRKIKALGEEKEKKLGRIDPRMIKMGSIKYYR 180
           SH+DVKYPIAVVGIFYKMGRPDPFLSKLSRKIKALGEEKEKKLGRIDPRMIKMGSIKYYR
Sbjct: 149 SHTDVKYPIAVVGIFYKMGRPDPFLSKLSRKIKALGEEKEKKLGRIDPRMIKMGSIKYYR 208

Query: 181 YMGSLTVPPCTEGVIWNINQKIGTVSREQVKLLRSAVHDSAEKNARPIQPHNGRQVDLYD 240
           YMGSLTVPPCTEGVIWNINQKIGTVSREQVKLLRSAVHDSAEKNARPIQP NGRQVDLYD
Sbjct: 209 YMGSLTVPPCTEGVIWNINQKIGTVSREQVKLLRSAVHDSAEKNARPIQPRNGRQVDLYD 268

Query: 241 PNPTHTPN 249
           PNPTHTPN
Sbjct: 269 PNPTHTPN 276

BLAST of MS019621 vs. NCBI nr
Match: XP_038905903.1 (alpha carbonic anhydrase 7-like [Benincasa hispida])

HSP 1 Score: 426.8 bits (1096), Expect = 1.3e-115
Identity = 204/248 (82.26%), Postives = 221/248 (89.11%), Query Frame = 0

Query: 1   EDESEFDYIRGSKKGPSKWGEIKREWERCKNGEMQSPIDLSSTRVKAIKTLGHLNNKYKP 60
           EDESEF+YIRGS+KGP +WGEIKREW +C NG+MQSPIDLS+ RVK +K LG L  KYKP
Sbjct: 27  EDESEFNYIRGSEKGPKRWGEIKREWSKCNNGDMQSPIDLSTKRVKVVKNLGQLKKKYKP 86

Query: 61  SNAILKNRGHDISIKWEGNAGSIKINDTNYLLQQIHWHSPSEHTLNGRRYDLELHLVHQS 120
           S AI+KNRGHDIS+KWE NAG I+IN T+YLLQQ HWHSPSEHTLNGRRYDLELHLVHQS
Sbjct: 87  SIAIVKNRGHDISVKWEENAGLIEINGTDYLLQQAHWHSPSEHTLNGRRYDLELHLVHQS 146

Query: 121 SHSDVKYPIAVVGIFYKMGRPDPFLSKLSRKIKALGEEKEKKLGRIDPRMIKMGSIKYYR 180
           S+ + KYPIAVVGIFY +GRPD FLSK+SRKIKAL E KE K G IDPR IKMG  KYYR
Sbjct: 147 SNPNAKYPIAVVGIFYMIGRPDSFLSKMSRKIKALNEVKEMKAGSIDPRWIKMGGKKYYR 206

Query: 181 YMGSLTVPPCTEGVIWNINQKIGTVSREQVKLLRSAVHDSAEKNARPIQPHNGRQVDLYD 240
           YMGSLTVPPCTEGVIWNIN+KIGTVSREQVKLLRSAVHDSAEKNARPIQPHNGRQVDLY+
Sbjct: 207 YMGSLTVPPCTEGVIWNINKKIGTVSREQVKLLRSAVHDSAEKNARPIQPHNGRQVDLYN 266

Query: 241 PNPTHTPN 249
           PNPT TPN
Sbjct: 267 PNPTQTPN 274

BLAST of MS019621 vs. NCBI nr
Match: XP_023527587.1 (alpha carbonic anhydrase 7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 424.9 bits (1091), Expect = 4.9e-115
Identity = 202/248 (81.45%), Postives = 221/248 (89.11%), Query Frame = 0

Query: 1   EDESEFDYIRGSKKGPSKWGEIKREWERCKNGEMQSPIDLSSTRVKAIKTLGHLNNKYKP 60
           EDESEF+YIRGSKKGP++WGEIK+EW  C NG+MQSPIDLSS RVK +K+LG L  KYKP
Sbjct: 32  EDESEFNYIRGSKKGPTRWGEIKKEWAMCNNGDMQSPIDLSSKRVKVVKSLGQLKRKYKP 91

Query: 61  SNAILKNRGHDISIKWEGNAGSIKINDTNYLLQQIHWHSPSEHTLNGRRYDLELHLVHQS 120
           S AILKNRGHDI+IKWEG+AGSI+IN TNY+LQQ HWHS SEHTLNGR YDLE+HLVHQS
Sbjct: 92  SMAILKNRGHDITIKWEGDAGSIEINGTNYVLQQAHWHSLSEHTLNGRSYDLEVHLVHQS 151

Query: 121 SHSDVKYPIAVVGIFYKMGRPDPFLSKLSRKIKALGEEKEKKLGRIDPRMIKMGSIKYYR 180
           S+ D KYPIAVVG+FYK+GRPD FL K+SRKIK LGEEKEKK GRIDPR IKMG  KYYR
Sbjct: 152 SNPDAKYPIAVVGVFYKIGRPDAFLGKMSRKIKELGEEKEKKAGRIDPRGIKMGGEKYYR 211

Query: 181 YMGSLTVPPCTEGVIWNINQKIGTVSREQVKLLRSAVHDSAEKNARPIQPHNGRQVDLYD 240
           YMGSLTVPPCTEGVIWNIN+K+ TVSREQV LLRSAVHDSAE+NARPIQPHNGR VDLYD
Sbjct: 212 YMGSLTVPPCTEGVIWNINKKVQTVSREQVSLLRSAVHDSAERNARPIQPHNGRHVDLYD 271

Query: 241 PNPTHTPN 249
           PNPTH  N
Sbjct: 272 PNPTHIIN 279

BLAST of MS019621 vs. NCBI nr
Match: XP_022935273.1 (alpha carbonic anhydrase 7-like [Cucurbita moschata])

HSP 1 Score: 421.4 bits (1082), Expect = 5.4e-114
Identity = 199/248 (80.24%), Postives = 221/248 (89.11%), Query Frame = 0

Query: 1   EDESEFDYIRGSKKGPSKWGEIKREWERCKNGEMQSPIDLSSTRVKAIKTLGHLNNKYKP 60
           EDESEF+YI+GSKKGP++WGEIK+EW  C NG+MQSPIDLSS RVK +K+LG L  KYKP
Sbjct: 32  EDESEFNYIKGSKKGPTRWGEIKKEWATCNNGDMQSPIDLSSKRVKVVKSLGQLKRKYKP 91

Query: 61  SNAILKNRGHDISIKWEGNAGSIKINDTNYLLQQIHWHSPSEHTLNGRRYDLELHLVHQS 120
           S AILKNRGHDI+IKWEG+AGSI+IN TNY+LQQ HWHS SEHTLNGR Y+LE+HLVHQS
Sbjct: 92  SMAILKNRGHDITIKWEGDAGSIEINGTNYVLQQAHWHSLSEHTLNGRSYELEVHLVHQS 151

Query: 121 SHSDVKYPIAVVGIFYKMGRPDPFLSKLSRKIKALGEEKEKKLGRIDPRMIKMGSIKYYR 180
           S+ D KYPIAVVG+FYK+GRPDPFL+K+SRKIK LGE KEK  GRIDPR IKMG  KYYR
Sbjct: 152 SNPDAKYPIAVVGVFYKIGRPDPFLAKMSRKIKELGEAKEKNAGRIDPRGIKMGGEKYYR 211

Query: 181 YMGSLTVPPCTEGVIWNINQKIGTVSREQVKLLRSAVHDSAEKNARPIQPHNGRQVDLYD 240
           YMGSLTVPPCTEGVIWNIN+K+ TVSREQV LLRSAVHDSAE+NARPIQPHNGR VDLYD
Sbjct: 212 YMGSLTVPPCTEGVIWNINKKVQTVSREQVSLLRSAVHDSAERNARPIQPHNGRHVDLYD 271

Query: 241 PNPTHTPN 249
           PNPTH  N
Sbjct: 272 PNPTHIIN 279

BLAST of MS019621 vs. NCBI nr
Match: XP_022983059.1 (alpha carbonic anhydrase 7-like [Cucurbita maxima])

HSP 1 Score: 421.0 bits (1081), Expect = 7.1e-114
Identity = 201/248 (81.05%), Postives = 223/248 (89.92%), Query Frame = 0

Query: 1   EDESEFDYIRGSKKGPSKWGEIKREWERCKNGEMQSPIDLSSTRVKAIKTLGHLNNKYKP 60
           EDESEF+YIR SKKGP++WGEIK+EW  C NG+MQSPIDLSS RVK +K+LG L  KYKP
Sbjct: 32  EDESEFNYIRESKKGPTRWGEIKKEWAMCNNGDMQSPIDLSSKRVKVVKSLGQLKKKYKP 91

Query: 61  SNAILKNRGHDISIKWEGNAGSIKINDTNYLLQQIHWHSPSEHTLNGRRYDLELHLVHQS 120
           S AILKNRGHDI+IKWEG+AGSI+IN TNY+LQQ HWHS SEHTLNGR YDLE+HLVHQS
Sbjct: 92  SMAILKNRGHDITIKWEGDAGSIEINGTNYVLQQAHWHSLSEHTLNGRSYDLEVHLVHQS 151

Query: 121 SHSDVKYPIAVVGIFYKMGRPDPFLSKLSRKIKALGEEKEKKLGRIDPRMIKMGSIKYYR 180
           S+ DVKYPIAVVG+FYK+GRPD FL+K+SRKIK LGEEKEKK GRIDPR IKMG  KYYR
Sbjct: 152 SNPDVKYPIAVVGMFYKIGRPDSFLTKMSRKIKELGEEKEKKAGRIDPRGIKMGGEKYYR 211

Query: 181 YMGSLTVPPCTEGVIWNINQKIGTVSREQVKLLRSAVHDSAEKNARPIQPHNGRQVDLYD 240
           YMGSLTVPPCTEGVIWNIN+K+ TVSREQ+ LLRSAVHDSAE+NARPIQPHNGRQVDLYD
Sbjct: 212 YMGSLTVPPCTEGVIWNINKKVQTVSREQLSLLRSAVHDSAERNARPIQPHNGRQVDLYD 271

Query: 241 PNPTHTPN 249
           PNPT+  N
Sbjct: 272 PNPTNIIN 279

BLAST of MS019621 vs. ExPASy Swiss-Prot
Match: Q8L817 (Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana OX=3702 GN=ACA7 PE=2 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 3.2e-77
Identity = 140/243 (57.61%), Postives = 175/243 (72.02%), Query Frame = 0

Query: 1   EDESEFDYIRGSKKGPSKWGEIKREWERCKNGEMQSPIDLSSTRVKAIKTLGHLNNKYKP 60
           EDE EF+Y +  +KGP +WGE+K EWE C  GEMQSPIDL + RV  +  LG LN  Y P
Sbjct: 35  EDEREFNYKKNDEKGPERWGELKPEWEMCGKGEMQSPIDLMNERVNIVSHLGRLNRDYNP 94

Query: 61  SNAILKNRGHDISIKWEGNAGSIKINDTNYLLQQIHWHSPSEHTLNGRRYDLELHLVHQS 120
           SNA LKNRGHDI +K+E  AG+IKIN   Y LQQ+HWHSPSEHT+NGRR+ LELH+VH+ 
Sbjct: 95  SNATLKNRGHDIMLKFEDGAGTIKINGFEYELQQLHWHSPSEHTINGRRFALELHMVHEG 154

Query: 121 SHSDVKYPIAVVGIFYKMGRPDPFLSKLSRKIKALG--EEKEKKLGRIDPRMIKMGSIKY 180
            +      +AVV + YK+GR D F+  L ++++ +   EE EK +G IDP  IK+GS KY
Sbjct: 155 RNR----RMAVVTVLYKIGRADTFIRSLEKELEGIAEMEEAEKNVGMIDPTKIKIGSRKY 214

Query: 181 YRYMGSLTVPPCTEGVIWNINQKIGTVSREQVKLLRSAVHDSAEKNARPIQPHNGRQVDL 240
           YRY GSLT PPCT+ V W++ +K+ TV+R+QVKLLR AVHD A  NARP+QP N R V L
Sbjct: 215 YRYTGSLTTPPCTQNVTWSVVRKVRTVTRKQVKLLRVAVHDDANSNARPVQPTNKRIVHL 273

Query: 241 YDP 242
           Y P
Sbjct: 275 YRP 273

BLAST of MS019621 vs. ExPASy Swiss-Prot
Match: F4JIK2 (Alpha carbonic anhydrase 4 OS=Arabidopsis thaliana OX=3702 GN=ACA4 PE=3 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 5.2e-67
Identity = 124/242 (51.24%), Postives = 164/242 (67.77%), Query Frame = 0

Query: 1   EDESEFDYIRGSKKGPSKWGEIKREWERCKNGEMQSPIDLSSTRVKAIKTLGHLNNKYKP 60
           +DE+ F Y + ++KGP  WG+I   W+ C  G  QSPIDL++ RV  I        +YKP
Sbjct: 31  DDETPFTYEQKTEKGPEGWGKINPHWKVCNTGRYQSPIDLTNERVSLIHDQA-WTRQYKP 90

Query: 61  SNAILKNRGHDISIKWEGNAGSIKINDTNYLLQQIHWHSPSEHTLNGRRYDLELHLVHQS 120
           + A++ NRGHDI + W+G+AG + I  T++ L Q HWHSPSEHT+NG RYDLELH+VH S
Sbjct: 91  APAVITNRGHDIMVSWKGDAGKMTIRKTDFNLVQCHWHSPSEHTVNGTRYDLELHMVHTS 150

Query: 121 SHSDVKYPIAVVGIFYKMGRPDPFLSKLSRKIKALGEEKEKKLGRIDPRMIKMGSIKYYR 180
           +        AV+G+ YK+G P+ FL+KL   IKA+G  KE  LG IDPR I+  + K+YR
Sbjct: 151 ARGRT----AVIGVLYKLGEPNEFLTKLLNGIKAVG-NKEINLGMIDPREIRFQTRKFYR 210

Query: 181 YMGSLTVPPCTEGVIWNINQKIGTVSREQVKLLRSAVHDSAEKNARPIQPHNGRQVDLYD 240
           Y+GSLTVPPCTEGVIW + +++ T+S EQ+  LR AV D  E N+RP+Q   GR V  YD
Sbjct: 211 YIGSLTVPPCTEGVIWTVVKRVNTISMEQITALRQAVDDGFETNSRPVQDSKGRSVWFYD 266

Query: 241 PN 243
           PN
Sbjct: 271 PN 266

BLAST of MS019621 vs. ExPASy Swiss-Prot
Match: Q84UV8 (Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3 OS=Nicotiana langsdorffii x Nicotiana sanderae OX=164110 GN=NEC3 PE=1 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 1.4e-64
Identity = 120/240 (50.00%), Postives = 167/240 (69.58%), Query Frame = 0

Query: 1   EDESEFDYIRGSKKGPSKWGEIKREWERCKNGEMQSPIDLSSTRVKAIKTLGHLNNKYKP 60
           +DESEF Y   S+ GP+ WG I+ +W+ C +G++QSPID+   R + +  L  L   YKP
Sbjct: 29  DDESEFSYDEKSENGPANWGNIRPDWKEC-SGKLQSPIDIFDLRAEVVSNLRILQKDYKP 88

Query: 61  SNAILKNRGHDISIKWEGNAGSIKINDTNYLLQQIHWHSPSEHTLNGRRYDLELHLVHQS 120
           SNA L NRGHDI ++ + + G +KIN+T Y L+Q+HWH+PSEHT+NG R++LE HLVH+S
Sbjct: 89  SNATLLNRGHDIMLRLD-DGGYLKINETQYQLKQLHWHTPSEHTINGERFNLEAHLVHES 148

Query: 121 SHSDVKYPIAVVGIFYKMGR-PDPFLSKLSRKIKALGEEK--EKKLGRIDPRMIKMGSIK 180
           ++        V+GI Y++G  PDPFLS +   +K    +K  E+ +G IDP  IK+   K
Sbjct: 149 NNG----KFVVIGIVYEIGLWPDPFLSMIENDLKVPANKKGIERGIGIIDPNQIKLDGKK 208

Query: 181 YYRYMGSLTVPPCTEGVIWNINQKIGTVSREQVKLLRSAVHDSAEKNARPIQPHNGRQVD 238
           Y+RY+GSLT PPCTEGV+W I++K+ TV+R Q+KLL+ AVHD  E NARP QP N R ++
Sbjct: 209 YFRYIGSLTTPPCTEGVVWIIDRKVKTVTRRQIKLLQEAVHDGFETNARPTQPENERYIN 262

BLAST of MS019621 vs. ExPASy Swiss-Prot
Match: Q9FM99 (Alpha carbonic anhydrase 8 OS=Arabidopsis thaliana OX=3702 GN=ACA8 PE=3 SV=3)

HSP 1 Score: 244.6 bits (623), Expect = 1.2e-63
Identity = 125/245 (51.02%), Postives = 165/245 (67.35%), Query Frame = 0

Query: 1   EDESEFDYIRGSKKGPSKWGEIKREWERCKNGEMQSPIDLSSTRVKAIKTLGHLNNKYKP 60
           EDE+EF Y     KGP+KWG +  EW+ C  G+MQSPIDL    V      G L ++Y P
Sbjct: 135 EDETEFSYETKGNKGPAKWGTLDAEWKMCGIGKMQSPIDLRDKNVVVSNKFGLLRSQYLP 194

Query: 61  SNAILKNRGHDISIKWE-GNAG-SIKINDTNYLLQQIHWHSPSEHTLNGRRYDLELHLVH 120
           SN  +KNRGHDI +K++ GN G  + I  T Y LQQ+HWHSPSEHT+NG+R+ LE HLVH
Sbjct: 195 SNTTIKNRGHDIMLKFKGGNKGIGVTIRGTRYQLQQLHWHSPSEHTINGKRFALEEHLVH 254

Query: 121 QSSHSDVKYPIAVVGIFYKMGRPDPFLSKLSRKIKALGE--EKEKKLGRIDPRMIKMGSI 180
           +S   D +Y  AVV   Y +G  DPFL  L +++K + +    E+ +G IDP+ +   S 
Sbjct: 255 ES--KDKRY--AVVAFLYNLGASDPFLFSLEKQLKKITDTHASEEHVGIIDPKKLSFESK 314

Query: 181 KYYRYMGSLTVPPCTEGVIWNINQKIGTVSREQVKLLRSAVHDSAEKNARPIQPHNGRQV 240
            YYRY GSLT PPC+E VIW+++++I TVS +QVKLLR AVHD+++ NARP+Q  N R+V
Sbjct: 315 HYYRYSGSLTAPPCSENVIWSVSKEIRTVSSKQVKLLRVAVHDASDSNARPLQAVNKRKV 374

Query: 241 DLYDP 242
            LY P
Sbjct: 375 YLYKP 375

BLAST of MS019621 vs. ExPASy Swiss-Prot
Match: F4HUC4 (Alpha carbonic anhydrase 5 OS=Arabidopsis thaliana OX=3702 GN=ACA5 PE=3 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 3.5e-63
Identity = 126/245 (51.43%), Postives = 164/245 (66.94%), Query Frame = 0

Query: 1   EDESEFDYIRGSKKGPSKWGEIKREWERCKNGEMQSPIDLSSTRVKAIKTLGHLNNKYKP 60
           EDE++F+Y +  +KGP  WG +K EW  C  G MQSPIDL+  RV     LG+L ++Y P
Sbjct: 30  EDETQFNYEKKGEKGPENWGRLKPEWAMCGKGNMQSPIDLTDKRVLIDHNLGYLRSQYLP 89

Query: 61  SNAILKNRGHDISIKWE-GNAG-SIKINDTNYLLQQIHWHSPSEHTLNGRRYDLELHLVH 120
           SNA +KNRGHDI +K+E GNAG  I IN T Y LQQIHWHSPSEHTLNG+R+ LE H+VH
Sbjct: 90  SNATIKNRGHDIMMKFEGGNAGLGITINGTEYKLQQIHWHSPSEHTLNGKRFVLEEHMVH 149

Query: 121 QSSHSDVKYPIAVVGIFYKMGRPDPFLSKLSRKIKALGE--EKEKKLGRIDPRMIKMGSI 180
           QS         AVV  FYK+G+PD FL  L R +K + +  E ++ +  + PR     S 
Sbjct: 150 QSKDG----RNAVVAFFYKLGKPDYFLLTLERYLKRITDTHESQEFVEMVHPRTFGFESK 209

Query: 181 KYYRYMGSLTVPPCTEGVIWNINQKIGTVSREQVKLLRSAVHDSAEKNARPIQPHNGRQV 240
            YYR++GSLT PPC+E VIW I++++ TV+ +Q+ +LR  VHD +  NARP+Q  N R V
Sbjct: 210 HYYRFIGSLTTPPCSENVIWTISKEMRTVTLKQLIMLRVTVHDQSNSNARPLQRKNERPV 269

Query: 241 DLYDP 242
            LY P
Sbjct: 270 ALYIP 270

BLAST of MS019621 vs. ExPASy TrEMBL
Match: A0A6J1CU30 (Carbonic anhydrase OS=Momordica charantia OX=3673 GN=LOC111014347 PE=3 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 1.2e-140
Identity = 246/248 (99.19%), Postives = 247/248 (99.60%), Query Frame = 0

Query: 1   EDESEFDYIRGSKKGPSKWGEIKREWERCKNGEMQSPIDLSSTRVKAIKTLGHLNNKYKP 60
           EDESEFDYIRGSKKGPSKWGEIKREWERCKNGEMQSPIDLSSTRVKAIKTLGHLNNKYKP
Sbjct: 29  EDESEFDYIRGSKKGPSKWGEIKREWERCKNGEMQSPIDLSSTRVKAIKTLGHLNNKYKP 88

Query: 61  SNAILKNRGHDISIKWEGNAGSIKINDTNYLLQQIHWHSPSEHTLNGRRYDLELHLVHQS 120
           SNAILKNRGHDISIKWEGNAGSIKINDTNYLLQQIHWHSPSEHTLNGRRYDLELHLVHQS
Sbjct: 89  SNAILKNRGHDISIKWEGNAGSIKINDTNYLLQQIHWHSPSEHTLNGRRYDLELHLVHQS 148

Query: 121 SHSDVKYPIAVVGIFYKMGRPDPFLSKLSRKIKALGEEKEKKLGRIDPRMIKMGSIKYYR 180
           SH+DVKYPIAVVGIFYKMGRPDPFLSKLSRKIKALGEEKEKKLGRIDPRMIKMGSIKYYR
Sbjct: 149 SHTDVKYPIAVVGIFYKMGRPDPFLSKLSRKIKALGEEKEKKLGRIDPRMIKMGSIKYYR 208

Query: 181 YMGSLTVPPCTEGVIWNINQKIGTVSREQVKLLRSAVHDSAEKNARPIQPHNGRQVDLYD 240
           YMGSLTVPPCTEGVIWNINQKIGTVSREQVKLLRSAVHDSAEKNARPIQP NGRQVDLYD
Sbjct: 209 YMGSLTVPPCTEGVIWNINQKIGTVSREQVKLLRSAVHDSAEKNARPIQPRNGRQVDLYD 268

Query: 241 PNPTHTPN 249
           PNPTHTPN
Sbjct: 269 PNPTHTPN 276

BLAST of MS019621 vs. ExPASy TrEMBL
Match: A0A6J1FA33 (Carbonic anhydrase OS=Cucurbita moschata OX=3662 GN=LOC111442211 PE=3 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 2.6e-114
Identity = 199/248 (80.24%), Postives = 221/248 (89.11%), Query Frame = 0

Query: 1   EDESEFDYIRGSKKGPSKWGEIKREWERCKNGEMQSPIDLSSTRVKAIKTLGHLNNKYKP 60
           EDESEF+YI+GSKKGP++WGEIK+EW  C NG+MQSPIDLSS RVK +K+LG L  KYKP
Sbjct: 32  EDESEFNYIKGSKKGPTRWGEIKKEWATCNNGDMQSPIDLSSKRVKVVKSLGQLKRKYKP 91

Query: 61  SNAILKNRGHDISIKWEGNAGSIKINDTNYLLQQIHWHSPSEHTLNGRRYDLELHLVHQS 120
           S AILKNRGHDI+IKWEG+AGSI+IN TNY+LQQ HWHS SEHTLNGR Y+LE+HLVHQS
Sbjct: 92  SMAILKNRGHDITIKWEGDAGSIEINGTNYVLQQAHWHSLSEHTLNGRSYELEVHLVHQS 151

Query: 121 SHSDVKYPIAVVGIFYKMGRPDPFLSKLSRKIKALGEEKEKKLGRIDPRMIKMGSIKYYR 180
           S+ D KYPIAVVG+FYK+GRPDPFL+K+SRKIK LGE KEK  GRIDPR IKMG  KYYR
Sbjct: 152 SNPDAKYPIAVVGVFYKIGRPDPFLAKMSRKIKELGEAKEKNAGRIDPRGIKMGGEKYYR 211

Query: 181 YMGSLTVPPCTEGVIWNINQKIGTVSREQVKLLRSAVHDSAEKNARPIQPHNGRQVDLYD 240
           YMGSLTVPPCTEGVIWNIN+K+ TVSREQV LLRSAVHDSAE+NARPIQPHNGR VDLYD
Sbjct: 212 YMGSLTVPPCTEGVIWNINKKVQTVSREQVSLLRSAVHDSAERNARPIQPHNGRHVDLYD 271

Query: 241 PNPTHTPN 249
           PNPTH  N
Sbjct: 272 PNPTHIIN 279

BLAST of MS019621 vs. ExPASy TrEMBL
Match: A0A6J1J682 (Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111481718 PE=3 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 3.4e-114
Identity = 201/248 (81.05%), Postives = 223/248 (89.92%), Query Frame = 0

Query: 1   EDESEFDYIRGSKKGPSKWGEIKREWERCKNGEMQSPIDLSSTRVKAIKTLGHLNNKYKP 60
           EDESEF+YIR SKKGP++WGEIK+EW  C NG+MQSPIDLSS RVK +K+LG L  KYKP
Sbjct: 32  EDESEFNYIRESKKGPTRWGEIKKEWAMCNNGDMQSPIDLSSKRVKVVKSLGQLKKKYKP 91

Query: 61  SNAILKNRGHDISIKWEGNAGSIKINDTNYLLQQIHWHSPSEHTLNGRRYDLELHLVHQS 120
           S AILKNRGHDI+IKWEG+AGSI+IN TNY+LQQ HWHS SEHTLNGR YDLE+HLVHQS
Sbjct: 92  SMAILKNRGHDITIKWEGDAGSIEINGTNYVLQQAHWHSLSEHTLNGRSYDLEVHLVHQS 151

Query: 121 SHSDVKYPIAVVGIFYKMGRPDPFLSKLSRKIKALGEEKEKKLGRIDPRMIKMGSIKYYR 180
           S+ DVKYPIAVVG+FYK+GRPD FL+K+SRKIK LGEEKEKK GRIDPR IKMG  KYYR
Sbjct: 152 SNPDVKYPIAVVGMFYKIGRPDSFLTKMSRKIKELGEEKEKKAGRIDPRGIKMGGEKYYR 211

Query: 181 YMGSLTVPPCTEGVIWNINQKIGTVSREQVKLLRSAVHDSAEKNARPIQPHNGRQVDLYD 240
           YMGSLTVPPCTEGVIWNIN+K+ TVSREQ+ LLRSAVHDSAE+NARPIQPHNGRQVDLYD
Sbjct: 212 YMGSLTVPPCTEGVIWNINKKVQTVSREQLSLLRSAVHDSAERNARPIQPHNGRQVDLYD 271

Query: 241 PNPTHTPN 249
           PNPT+  N
Sbjct: 272 PNPTNIIN 279

BLAST of MS019621 vs. ExPASy TrEMBL
Match: A0A0A0LAM4 (Carbonic anhydrase OS=Cucumis sativus OX=3659 GN=Csa_3G742880 PE=3 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 4.5e-114
Identity = 200/248 (80.65%), Postives = 222/248 (89.52%), Query Frame = 0

Query: 1   EDESEFDYIRGSKKGPSKWGEIKREWERCKNGEMQSPIDLSSTRVKAIKTLGHLNNKYKP 60
           EDESEFDY +GSKKGPSKWG+IKREWE+C NG MQSPIDLS+ RVK +K LG L NKYKP
Sbjct: 28  EDESEFDYRKGSKKGPSKWGDIKREWEKCNNGNMQSPIDLSNKRVKVVKHLGQLKNKYKP 87

Query: 61  SNAILKNRGHDISIKWEGNAGSIKINDTNYLLQQIHWHSPSEHTLNGRRYDLELHLVHQS 120
           S AI+KNRGHDIS+KWE +AGSI+IN T+YLLQQ HWHSPSEHTLNGRRYDLE+HLVHQS
Sbjct: 88  SIAIIKNRGHDISVKWEEDAGSIEINGTDYLLQQAHWHSPSEHTLNGRRYDLEVHLVHQS 147

Query: 121 SHSDVKYPIAVVGIFYKMGRPDPFLSKLSRKIKALGEEKEKKLGRIDPRMIKMGSIKYYR 180
           S+ + KYPIAVVG FYK+GRPD FLSKL+RKIKAL E+KE K G I+PR IK G +KYYR
Sbjct: 148 SNPNAKYPIAVVGFFYKIGRPDSFLSKLNRKIKALNEKKEIKAGIINPRWIKNGGMKYYR 207

Query: 181 YMGSLTVPPCTEGVIWNINQKIGTVSREQVKLLRSAVHDSAEKNARPIQPHNGRQVDLYD 240
           Y+GSLTVPPCTEGVIWNI +KIGTVSR+QVKLLRSAVHDSAEKNARPIQPHNGR +DLYD
Sbjct: 208 YIGSLTVPPCTEGVIWNIKKKIGTVSRKQVKLLRSAVHDSAEKNARPIQPHNGRHIDLYD 267

Query: 241 PNPTHTPN 249
           PNPTHT N
Sbjct: 268 PNPTHTLN 275

BLAST of MS019621 vs. ExPASy TrEMBL
Match: A0A5A7TM78 (Carbonic anhydrase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002880 PE=3 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 2.2e-113
Identity = 200/248 (80.65%), Postives = 221/248 (89.11%), Query Frame = 0

Query: 1   EDESEFDYIRGSKKGPSKWGEIKREWERCKNGEMQSPIDLSSTRVKAIKTLGHLNNKYKP 60
           EDESEFDY +GSKKGPSKWG+IKREWE+CKNG MQSPIDLS+ RVK +K+LG L NKYKP
Sbjct: 27  EDESEFDYRKGSKKGPSKWGDIKREWEKCKNGNMQSPIDLSNKRVKVVKSLGQLKNKYKP 86

Query: 61  SNAILKNRGHDISIKWEGNAGSIKINDTNYLLQQIHWHSPSEHTLNGRRYDLELHLVHQS 120
           S AI+KNRGHDIS+KWE +AGSI+IN T YLLQQ HWHSPSEHTLNGR YDLE+HLVHQS
Sbjct: 87  SIAIIKNRGHDISVKWEEDAGSIEINGTEYLLQQAHWHSPSEHTLNGRSYDLEVHLVHQS 146

Query: 121 SHSDVKYPIAVVGIFYKMGRPDPFLSKLSRKIKALGEEKEKKLGRIDPRMIKMGSIKYYR 180
           S+ + KYPIAVVG FYK+GRPD FLSKL+RKIKAL E+KE K G I+PR IK G +KYYR
Sbjct: 147 SNPNAKYPIAVVGFFYKIGRPDSFLSKLTRKIKALNEKKETKAGIINPRWIKNGGMKYYR 206

Query: 181 YMGSLTVPPCTEGVIWNINQKIGTVSREQVKLLRSAVHDSAEKNARPIQPHNGRQVDLYD 240
           Y+GSLTVPPCTEGVIWNIN+KIGTVS +QVKLLRSAVHDSAEKNARPIQPHNGR V LYD
Sbjct: 207 YIGSLTVPPCTEGVIWNINKKIGTVSGKQVKLLRSAVHDSAEKNARPIQPHNGRHVVLYD 266

Query: 241 PNPTHTPN 249
           PNPTHT N
Sbjct: 267 PNPTHTLN 274

BLAST of MS019621 vs. TAIR 10
Match: AT1G08080.1 (alpha carbonic anhydrase 7 )

HSP 1 Score: 289.7 bits (740), Expect = 2.3e-78
Identity = 140/243 (57.61%), Postives = 175/243 (72.02%), Query Frame = 0

Query: 1   EDESEFDYIRGSKKGPSKWGEIKREWERCKNGEMQSPIDLSSTRVKAIKTLGHLNNKYKP 60
           EDE EF+Y +  +KGP +WGE+K EWE C  GEMQSPIDL + RV  +  LG LN  Y P
Sbjct: 35  EDEREFNYKKNDEKGPERWGELKPEWEMCGKGEMQSPIDLMNERVNIVSHLGRLNRDYNP 94

Query: 61  SNAILKNRGHDISIKWEGNAGSIKINDTNYLLQQIHWHSPSEHTLNGRRYDLELHLVHQS 120
           SNA LKNRGHDI +K+E  AG+IKIN   Y LQQ+HWHSPSEHT+NGRR+ LELH+VH+ 
Sbjct: 95  SNATLKNRGHDIMLKFEDGAGTIKINGFEYELQQLHWHSPSEHTINGRRFALELHMVHEG 154

Query: 121 SHSDVKYPIAVVGIFYKMGRPDPFLSKLSRKIKALG--EEKEKKLGRIDPRMIKMGSIKY 180
            +      +AVV + YK+GR D F+  L ++++ +   EE EK +G IDP  IK+GS KY
Sbjct: 155 RNR----RMAVVTVLYKIGRADTFIRSLEKELEGIAEMEEAEKNVGMIDPTKIKIGSRKY 214

Query: 181 YRYMGSLTVPPCTEGVIWNINQKIGTVSREQVKLLRSAVHDSAEKNARPIQPHNGRQVDL 240
           YRY GSLT PPCT+ V W++ +K+ TV+R+QVKLLR AVHD A  NARP+QP N R V L
Sbjct: 215 YRYTGSLTTPPCTQNVTWSVVRKVRTVTRKQVKLLRVAVHDDANSNARPVQPTNKRIVHL 273

Query: 241 YDP 242
           Y P
Sbjct: 275 YRP 273

BLAST of MS019621 vs. TAIR 10
Match: AT4G20990.1 (alpha carbonic anhydrase 4 )

HSP 1 Score: 255.8 bits (652), Expect = 3.7e-68
Identity = 124/242 (51.24%), Postives = 164/242 (67.77%), Query Frame = 0

Query: 1   EDESEFDYIRGSKKGPSKWGEIKREWERCKNGEMQSPIDLSSTRVKAIKTLGHLNNKYKP 60
           +DE+ F Y + ++KGP  WG+I   W+ C  G  QSPIDL++ RV  I        +YKP
Sbjct: 31  DDETPFTYEQKTEKGPEGWGKINPHWKVCNTGRYQSPIDLTNERVSLIHDQA-WTRQYKP 90

Query: 61  SNAILKNRGHDISIKWEGNAGSIKINDTNYLLQQIHWHSPSEHTLNGRRYDLELHLVHQS 120
           + A++ NRGHDI + W+G+AG + I  T++ L Q HWHSPSEHT+NG RYDLELH+VH S
Sbjct: 91  APAVITNRGHDIMVSWKGDAGKMTIRKTDFNLVQCHWHSPSEHTVNGTRYDLELHMVHTS 150

Query: 121 SHSDVKYPIAVVGIFYKMGRPDPFLSKLSRKIKALGEEKEKKLGRIDPRMIKMGSIKYYR 180
           +        AV+G+ YK+G P+ FL+KL   IKA+G  KE  LG IDPR I+  + K+YR
Sbjct: 151 ARGRT----AVIGVLYKLGEPNEFLTKLLNGIKAVG-NKEINLGMIDPREIRFQTRKFYR 210

Query: 181 YMGSLTVPPCTEGVIWNINQKIGTVSREQVKLLRSAVHDSAEKNARPIQPHNGRQVDLYD 240
           Y+GSLTVPPCTEGVIW + +++ T+S EQ+  LR AV D  E N+RP+Q   GR V  YD
Sbjct: 211 YIGSLTVPPCTEGVIWTVVKRVNTISMEQITALRQAVDDGFETNSRPVQDSKGRSVWFYD 266

Query: 241 PN 243
           PN
Sbjct: 271 PN 266

BLAST of MS019621 vs. TAIR 10
Match: AT1G08065.1 (alpha carbonic anhydrase 5 )

HSP 1 Score: 243.0 bits (619), Expect = 2.5e-64
Identity = 126/245 (51.43%), Postives = 164/245 (66.94%), Query Frame = 0

Query: 1   EDESEFDYIRGSKKGPSKWGEIKREWERCKNGEMQSPIDLSSTRVKAIKTLGHLNNKYKP 60
           EDE++F+Y +  +KGP  WG +K EW  C  G MQSPIDL+  RV     LG+L ++Y P
Sbjct: 30  EDETQFNYEKKGEKGPENWGRLKPEWAMCGKGNMQSPIDLTDKRVLIDHNLGYLRSQYLP 89

Query: 61  SNAILKNRGHDISIKWE-GNAG-SIKINDTNYLLQQIHWHSPSEHTLNGRRYDLELHLVH 120
           SNA +KNRGHDI +K+E GNAG  I IN T Y LQQIHWHSPSEHTLNG+R+ LE H+VH
Sbjct: 90  SNATIKNRGHDIMMKFEGGNAGLGITINGTEYKLQQIHWHSPSEHTLNGKRFVLEEHMVH 149

Query: 121 QSSHSDVKYPIAVVGIFYKMGRPDPFLSKLSRKIKALGE--EKEKKLGRIDPRMIKMGSI 180
           QS         AVV  FYK+G+PD FL  L R +K + +  E ++ +  + PR     S 
Sbjct: 150 QSKDG----RNAVVAFFYKLGKPDYFLLTLERYLKRITDTHESQEFVEMVHPRTFGFESK 209

Query: 181 KYYRYMGSLTVPPCTEGVIWNINQKIGTVSREQVKLLRSAVHDSAEKNARPIQPHNGRQV 240
            YYR++GSLT PPC+E VIW I++++ TV+ +Q+ +LR  VHD +  NARP+Q  N R V
Sbjct: 210 HYYRFIGSLTTPPCSENVIWTISKEMRTVTLKQLIMLRVTVHDQSNSNARPLQRKNERPV 269

Query: 241 DLYDP 242
            LY P
Sbjct: 270 ALYIP 270

BLAST of MS019621 vs. TAIR 10
Match: AT2G28210.1 (alpha carbonic anhydrase 2 )

HSP 1 Score: 226.5 bits (576), Expect = 2.4e-59
Identity = 110/207 (53.14%), Postives = 145/207 (70.05%), Query Frame = 0

Query: 1   EDESEFDYIRGSKKGPSKWGEIKREWERCKNGEMQSPIDLSSTRVKAIKTLGHLNNKYKP 60
           EDE EF Y    + GP+KWG+++ EW+ C  GEMQSPIDL + RV+ +  L  L   YKP
Sbjct: 6   EDEHEFSYEWNQENGPAKWGKLRPEWKMCGKGEMQSPIDLMNKRVRLVTHLKKLTRHYKP 65

Query: 61  SNAILKNRGHDISIKW-EGNAGSIKINDTNYLLQQIHWHSPSEHTLNGRRYDLELHLVHQ 120
            NA LKNRGHD+ +K+ E  +GSI +N T Y L Q+HWHSPSEHT+NGRR+ LELH+VH+
Sbjct: 66  CNATLKNRGHDMMLKFGEEGSGSITVNGTEYKLLQLHWHSPSEHTMNGRRFALELHMVHE 125

Query: 121 SSHSDVKYPIAVVGIFYKMGRPDPFLSKLSRKIKALGE--EKEKKLGRIDPRMIKMGSIK 180
               ++   +AVV + YK+GRPD FL  L  K+ A+ +  E EK +  IDPR IK+GS K
Sbjct: 126 ----NINGSLAVVTVLYKIGRPDSFLGLLENKLSAITDQNEAEKYVDVIDPRDIKIGSRK 185

Query: 181 YYRYMGSLTVPPCTEGVIWNINQKIGT 205
           +YRY+GSLT PPCT+ VIW + +K+ T
Sbjct: 186 FYRYIGSLTTPPCTQNVIWTVVKKVNT 208

BLAST of MS019621 vs. TAIR 10
Match: AT5G04180.1 (alpha carbonic anhydrase 3 )

HSP 1 Score: 225.7 bits (574), Expect = 4.1e-59
Identity = 119/250 (47.60%), Postives = 159/250 (63.60%), Query Frame = 0

Query: 1   EDESEFDYIRGSKKGPSKWGEIKREWERCKNGEMQSPIDLSSTRVKAI-KTLGHLNNKYK 60
           E E+EF Y  G    PSKW  IK EW+ C  G+ QSPI+L+    + +  +   L   YK
Sbjct: 21  ETETEFHYKPGEIADPSKWSSIKAEWKICGTGKRQSPINLTPKIARIVHNSTEILQTYYK 80

Query: 61  PSNAILKNRGHDISIKWEGNAGSIKINDTNYLLQQIHWHSPSEHTLNGRRYDLELHLVHQ 120
           P  AILKNRG D+ +KWE +AG I INDT+Y L Q HWH+PSEH L+G+R  +ELH+VH+
Sbjct: 81  PVEAILKNRGFDMKVKWEDDAGKIVINDTDYKLVQSHWHAPSEHFLDGQRLAMELHMVHK 140

Query: 121 SSHSDVKYPIAVVGIFYKMGRPDPFLSKLSRKIKALG--EEKEKKLGRIDPRMIKMGSIK 180
           S    V+  +AV+G+ ++ G P+ F+S++  KI  +   ++ E  +G+IDPR       K
Sbjct: 141 S----VEGHLAVIGVLFREGEPNAFISRIMDKIHKIADVQDGEVSIGKIDPREFGWDLTK 200

Query: 181 YYRYMGSLTVPPCTEGVIWNINQKIGTVSREQVKLLRSAVHDSAEKNARPIQPHNGRQVD 240
           +Y Y GSLT PPCTE V+W I  K+GTVSREQ+ +L  A     EKNARP QP NGR V 
Sbjct: 201 FYEYRGSLTTPPCTEDVMWTIINKVGTVSREQIDVLTDARRGGYEKNARPAQPLNGRLVY 260

Query: 241 LYD---PNPT 245
           L +   P+PT
Sbjct: 261 LNEQSSPSPT 266

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022144728.12.6e-14099.19alpha carbonic anhydrase 7-like [Momordica charantia][more]
XP_038905903.11.3e-11582.26alpha carbonic anhydrase 7-like [Benincasa hispida][more]
XP_023527587.14.9e-11581.45alpha carbonic anhydrase 7-like [Cucurbita pepo subsp. pepo][more]
XP_022935273.15.4e-11480.24alpha carbonic anhydrase 7-like [Cucurbita moschata][more]
XP_022983059.17.1e-11481.05alpha carbonic anhydrase 7-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8L8173.2e-7757.61Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana OX=3702 GN=ACA7 PE=2 SV=1[more]
F4JIK25.2e-6751.24Alpha carbonic anhydrase 4 OS=Arabidopsis thaliana OX=3702 GN=ACA4 PE=3 SV=1[more]
Q84UV81.4e-6450.00Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3 OS... [more]
Q9FM991.2e-6351.02Alpha carbonic anhydrase 8 OS=Arabidopsis thaliana OX=3702 GN=ACA8 PE=3 SV=3[more]
F4HUC43.5e-6351.43Alpha carbonic anhydrase 5 OS=Arabidopsis thaliana OX=3702 GN=ACA5 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CU301.2e-14099.19Carbonic anhydrase OS=Momordica charantia OX=3673 GN=LOC111014347 PE=3 SV=1[more]
A0A6J1FA332.6e-11480.24Carbonic anhydrase OS=Cucurbita moschata OX=3662 GN=LOC111442211 PE=3 SV=1[more]
A0A6J1J6823.4e-11481.05Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111481718 PE=3 SV=1[more]
A0A0A0LAM44.5e-11480.65Carbonic anhydrase OS=Cucumis sativus OX=3659 GN=Csa_3G742880 PE=3 SV=1[more]
A0A5A7TM782.2e-11380.65Carbonic anhydrase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G0... [more]
Match NameE-valueIdentityDescription
AT1G08080.12.3e-7857.61alpha carbonic anhydrase 7 [more]
AT4G20990.13.7e-6851.24alpha carbonic anhydrase 4 [more]
AT1G08065.12.5e-6451.43alpha carbonic anhydrase 5 [more]
AT2G28210.12.4e-5953.14alpha carbonic anhydrase 2 [more]
AT5G04180.14.1e-5947.60alpha carbonic anhydrase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001148Alpha carbonic anhydrase domainSMARTSM01057Carb_anhydrase_2acoord: 8..237
e-value: 1.2E-62
score: 224.1
IPR001148Alpha carbonic anhydrase domainPFAMPF00194Carb_anhydrasecoord: 12..237
e-value: 4.3E-44
score: 151.0
IPR001148Alpha carbonic anhydrase domainPROSITEPS51144ALPHA_CA_2coord: 4..240
score: 45.507305
IPR036398Alpha carbonic anhydrase domain superfamilyGENE3D3.10.200.10Alpha carbonic anhydrasecoord: 2..243
e-value: 4.2E-76
score: 257.6
IPR036398Alpha carbonic anhydrase domain superfamilySUPERFAMILY51069Carbonic anhydrasecoord: 5..236
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 220..248
NoneNo IPR availablePANTHERPTHR18952:SF201ALPHA CARBONIC ANHYDRASE 3coord: 1..243
IPR023561Carbonic anhydrase, alpha-classPANTHERPTHR18952CARBONIC ANHYDRASEcoord: 1..243
IPR018338Carbonic anhydrase, alpha-class, conserved sitePROSITEPS00162ALPHA_CA_1coord: 101..117
IPR041891Carbonic anhydrase, prokaryotic-likeCDDcd03124alpha_CA_prokaryotic_likecoord: 15..236
e-value: 2.85974E-95
score: 276.46

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS019621.1MS019621.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004089 carbonate dehydratase activity
molecular_function GO:0008270 zinc ion binding