MS019618 (gene) Bitter gourd (TR) v1

Overview


Name	MS019618
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	alpha carbonic anhydrase 4-like
Location	scaffold729: 1045573 .. 1047656 (+)
RNA-Seq Expression	MS019618
Synteny	MS019618

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAACCCAACAAACAAAACCCACCTTTTAATTCTCATCTTTGCAATAATAATCTCTTGCTTTCTTTGCTTATCATCATCAGACTCAGTCTCAGCAATATTATTATCTGATGATGGTGATGAAGGGTTCAGTTATGATCCATGTAGTGAGAGAGGGCCTAAGCATTGGGGAGATCTTAAGGGAGAATGGGCAATGTGTAAAAATGGCAAAATGCAATCTCCCGTAGCTTTGTCTTTTTGGAATGCAAGATTCACAAAAGTTTTTGGTCATCAGCTCACCAGAAACCACTGGCCTGCTAATGCAGTTCTAAAAAACAATGGCCATGGAGTTGTTGTAAGCACAAAAACACAAATCATTTCTCTCTTTAATTTCTCGCAATTTCATCACTGTTTTACAGTAATGGAGAAAAGTTCACAAATTAGTTTTTTTTCTTTTCGTTTTCTTTATAATCGTTTATACCAACTTTCAACCTAAAAGAGAAGTTACCAATCTCTTAATCATTTAGTTAAGAGATGGACTTCTTTTCTAGAAAAAAAATGTGGTAGAGAGATTTTTGTAGTTTTTTTAATTGCAATTACTTTTTTTTTTCTCCTTATTAAACAGAATTTTAAATAAAGGCAACAAGAGTCTTAATTTTTTGTTTCACTTAGGTAAGGAAAATTAGTTGGCACCATTTATAAAAAAAGTTTAGGGAAACTGTAACATAAAATAAATATGTTTTATAAAAATATCATTACATTTTGTTTTTTTTAAATGTCACACGAGTGAGTTTGAGTTTATCTTATGTGATTGTATAGATTTGCAATTTATACTACTTTTGTAAAATTTTAAACCTAATACTACATATTATATAAATACATATTCATTTTGTGATATTTTTTTAATTCTCTCTTTTTAAATTTTAAACATATACTTTTTTTCAATCTATCAAATACTCAAACTAGTAAATACATTTGAAGCCGTATGTTTCATTCTTTGTCATATTTATAATAATTTTTCTAACTCTTACTTTTATAATATTTTATCCTAAACAAATTAAATTGAACAAACGTAGGTTGAATGGGGAGGAGATGCTGGATCAATCAAGATCAACGACATCTATTACAATTTGAGAAATTATCATTGGCACCATCCTTCTGAGCACACCAAAGATGGCAAAACGTCTTTTCCCTCTCTCGCTACTATATTATAACAGTATTTATACGGTCTTGTTATTATTATTATTATCATTGTTGTTTTGATGATAAATATATATTGTTAAATATTATTAGGTACCCTTTGGAGCTTCATATGGTACACACCAACATGGTGACAGGTAACATAGCTGTTATAGGCGTCTTATATGAGTTTGGTCCATCTGATCCACTCCTCAGTTTGGTAAATAAATTCCACCAACAATCCTATACCTTAAATCACTAATAGAATATTGATCAAAGATCGAGAACTTTATTAAACAATAGTTTTTAAACTATTTTTTCAATTATATACGTCTAAATTAATATCTTTATTCTATTAAATCTTTTTCAAAATTCAAAGATTTGTCAGACACAACACTCATTAATTATAATATTAATTGATATATCTCAGATAGAAAAGGACTTAAAACGTCTCAACATCCAAGGAGGTGAGATATGGCTTGGACCAGTGGATCCTAGACTTGTATGGCTTCGTAGTTTGAGTGATAGATATGCGGGATACATGGGATCACTCACCACTCCTCCTTGTACGGAAGGTGTCATTTGGACTGTAATGGAAAGGGTATTCATCTTTGCTTGCTGTTTGCCTTCTTTTAATCCACATAATTATCTTAATCCTTTCCTCTCATTCTAATTAATTATAACTCTCTTCAGGTTCATACTGTCTCACCAGACCAACTCGAACTTCTTAAACAGGCCGTTGTGGAGGTAAATATGTACTATAGCTCATATCTATCGCTTAAGCTTTTGGGTTTAGTTTAAACTTCTGTAATGTATTTTTTATATGAATTTGTATAGGAGAATATTGCGAGGCCACTGCAAGACGTTAATGGTCGACCCGTTTGGTACTATAACCCCTTGTTGAGGAAGAGTGCAGCCGATCAG

mRNA sequence

ATGGAGAACCCAACAAACAAAACCCACCTTTTAATTCTCATCTTTGCAATAATAATCTCTTGCTTTCTTTGCTTATCATCATCAGACTCAGTCTCAGCAATATTATTATCTGATGATGGTGATGAAGGGTTCAGTTATGATCCATGTAGTGAGAGAGGGCCTAAGCATTGGGGAGATCTTAAGGGAGAATGGGCAATGTGTAAAAATGGCAAAATGCAATCTCCCGTAGCTTTGTCTTTTTGGAATGCAAGATTCACAAAAGTTTTTGGTCATCAGCTCACCAGAAACCACTGGCCTGCTAATGCAGTTCTAAAAAACAATGGCCATGGAGTTGTTGTTGAATGGGGAGGAGATGCTGGATCAATCAAGATCAACGACATCTATTACAATTTGAGAAATTATCATTGGCACCATCCTTCTGAGCACACCAAAGATGGCAAAACGTACCCTTTGGAGCTTCATATGGTACACACCAACATGGTGACAGGTAACATAGCTGTTATAGGCGTCTTATATGAGTTTGGTCCATCTGATCCACTCCTCAGTTTGATAGAAAAGGACTTAAAACGTCTCAACATCCAAGGAGGTGAGATATGGCTTGGACCAGTGGATCCTAGACTTGTATGGCTTCGTAGTTTGAGTGATAGATATGCGGGATACATGGGATCACTCACCACTCCTCCTTGTACGGAAGGTGTCATTTGGACTGTAATGGAAAGGGTTCATACTGTCTCACCAGACCAACTCGAACTTCTTAAACAGGCCGTTGTGGAGGAGAATATTGCGAGGCCACTGCAAGACGTTAATGGTCGACCCGTTTGGTACTATAACCCCTTGTTGAGGAAGAGTGCAGCCGATCAG

Coding sequence (CDS)

ATGGAGAACCCAACAAACAAAACCCACCTTTTAATTCTCATCTTTGCAATAATAATCTCTTGCTTTCTTTGCTTATCATCATCAGACTCAGTCTCAGCAATATTATTATCTGATGATGGTGATGAAGGGTTCAGTTATGATCCATGTAGTGAGAGAGGGCCTAAGCATTGGGGAGATCTTAAGGGAGAATGGGCAATGTGTAAAAATGGCAAAATGCAATCTCCCGTAGCTTTGTCTTTTTGGAATGCAAGATTCACAAAAGTTTTTGGTCATCAGCTCACCAGAAACCACTGGCCTGCTAATGCAGTTCTAAAAAACAATGGCCATGGAGTTGTTGTTGAATGGGGAGGAGATGCTGGATCAATCAAGATCAACGACATCTATTACAATTTGAGAAATTATCATTGGCACCATCCTTCTGAGCACACCAAAGATGGCAAAACGTACCCTTTGGAGCTTCATATGGTACACACCAACATGGTGACAGGTAACATAGCTGTTATAGGCGTCTTATATGAGTTTGGTCCATCTGATCCACTCCTCAGTTTGATAGAAAAGGACTTAAAACGTCTCAACATCCAAGGAGGTGAGATATGGCTTGGACCAGTGGATCCTAGACTTGTATGGCTTCGTAGTTTGAGTGATAGATATGCGGGATACATGGGATCACTCACCACTCCTCCTTGTACGGAAGGTGTCATTTGGACTGTAATGGAAAGGGTTCATACTGTCTCACCAGACCAACTCGAACTTCTTAAACAGGCCGTTGTGGAGGAGAATATTGCGAGGCCACTGCAAGACGTTAATGGTCGACCCGTTTGGTACTATAACCCCTTGTTGAGGAAGAGTGCAGCCGATCAG

Protein sequence

MENPTNKTHLLILIFAIIISCFLCLSSSDSVSAILLSDDGDEGFSYDPCSERGPKHWGDLKGEWAMCKNGKMQSPVALSFWNARFTKVFGHQLTRNHWPANAVLKNNGHGVVVEWGGDAGSIKINDIYYNLRNYHWHHPSEHTKDGKTYPLELHMVHTNMVTGNIAVIGVLYEFGPSDPLLSLIEKDLKRLNIQGGEIWLGPVDPRLVWLRSLSDRYAGYMGSLTTPPCTEGVIWTVMERVHTVSPDQLELLKQAVVEENIARPLQDVNGRPVWYYNPLLRKSAADQ

Homology

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BLAST of MS019618 vs. NCBI nr
Match: XP_022144730.1 (alpha carbonic anhydrase 4-like [Momordica charantia])

HSP 1 Score: 604.7 bits (1558), Expect = 4.0e-169
Identity = 284/287 (98.95%), Postives = 286/287 (99.65%), Query Frame = 0


Query: 1   MENPTNKTHLLILIFAIIISCFLCLSSSDSVSAILLSDDGDEGFSYDPCSERGPKHWGDL 60
           MENPTNKTHLLILIFAIIISCFLCLSSSDSVSAILLSDDGDEGFSYDPCSERGPKHWGDL
Sbjct: 1   MENPTNKTHLLILIFAIIISCFLCLSSSDSVSAILLSDDGDEGFSYDPCSERGPKHWGDL 60

Query: 61  KGEWAMCKNGKMQSPVALSFWNARFTKVFGHQLTRNHWPANAVLKNNGHGVVVEWGGDAG 120
           K EWAMCKNGKMQSPVALSFWNARFTKVFGHQLTRNHWPANAVLKNNGHGVVVEWGGDAG
Sbjct: 61  KEEWAMCKNGKMQSPVALSFWNARFTKVFGHQLTRNHWPANAVLKNNGHGVVVEWGGDAG 120

Query: 121 SIKINDIYYNLRNYHWHHPSEHTKDGKTYPLELHMVHTNMVTGNIAVIGVLYEFGPSDPL 180
           SIKINDIYYNLRNYHWHHPSEHTKDGKTYPLELHMVHTNMVTGNIAVIGVLYEFGPSDPL
Sbjct: 121 SIKINDIYYNLRNYHWHHPSEHTKDGKTYPLELHMVHTNMVTGNIAVIGVLYEFGPSDPL 180

Query: 181 LSLIEKDLKRLNIQGGEIWLGPVDPRLVWLRSLSDRYAGYMGSLTTPPCTEGVIWTVMER 240
           LSLIEKDLKRLNI+GGEIWLGPVDPRLVWLRSLSD+YAGYMGSLTTPPCTEGVIWTVMER
Sbjct: 181 LSLIEKDLKRLNIEGGEIWLGPVDPRLVWLRSLSDKYAGYMGSLTTPPCTEGVIWTVMER 240

Query: 241 VHTVSPDQLELLKQAVVEENIARPLQDVNGRPVWYYNPLLRKSAADQ 288
           VHTVSPDQLELLKQAVVEENIARPLQDVNGRPVWYYNPLLRKSAADQ
Sbjct: 241 VHTVSPDQLELLKQAVVEENIARPLQDVNGRPVWYYNPLLRKSAADQ 287

[Close]

BLAST of MS019618 vs. NCBI nr
Match: XP_022144735.1 (alpha carbonic anhydrase 4-like [Momordica charantia])

HSP 1 Score: 523.5 bits (1347), Expect = 1.2e-144
Identity = 250/286 (87.41%), Postives = 262/286 (91.61%), Query Frame = 0


Query: 1   MENPTNKTHLLILIFAIIISCFLCLSSSDSVSAILLSDDGDEGFSYDPCSERGPKHWGDL 60
           MENPTNKTH+LILIFAI ISCFLCLSSS SVSAI LS+DGDEGFSYDPCSERGPKHWG+L
Sbjct: 1   MENPTNKTHILILIFAITISCFLCLSSSVSVSAI-LSNDGDEGFSYDPCSERGPKHWGNL 60

Query: 61  KGEWAMCKNGKMQSPVALSFWNARFTKVFGHQLTRNHWPANAVLKNNGHGVVVEWGGDAG 120
           K EWAMCKNGKMQSP+ALSFWNARFT VFGH++TRNHWPANAVLKNNGH VVVEW GDAG
Sbjct: 61  KEEWAMCKNGKMQSPIALSFWNARFTNVFGHRITRNHWPANAVLKNNGHEVVVEWKGDAG 120

Query: 121 SIKINDIYYNLRNYHWHHPSEHTKDGKTYPLELHMVHTNMVTGNIAVIGVLYEFGPSDPL 180
           SIKINDI Y+L+NYHWHHPSEH KDGKTYPLELHMVHTNMVTGN AVIGVLYEFGPSDPL
Sbjct: 121 SIKINDIDYHLKNYHWHHPSEHIKDGKTYPLELHMVHTNMVTGNKAVIGVLYEFGPSDPL 180

Query: 181 LSLIEKDLKRLNIQGGEIWLGPVDPRLVWLRSLSDRYAGYMGSLTTPPCTEGVIWTVMER 240
           LSLIEKDLK LNI+GGE W G VDPRLVWL S S+ YAGYMGSLTTPPCTEGVIWTVMER
Sbjct: 181 LSLIEKDLKHLNIEGGETWFGEVDPRLVWLYSSSEGYAGYMGSLTTPPCTEGVIWTVMER 240

Query: 241 VHTVSPDQLELLKQAVVEENIARPLQDVNGRPVWYYNPLLRKSAAD 287
           VHTVSPDQLELLK AVVEEN ARPLQDV G  V YY+P LRKSAA+
Sbjct: 241 VHTVSPDQLELLKLAVVEENTARPLQDVKGPRVGYYDPSLRKSAAE 285

[Close]

BLAST of MS019618 vs. NCBI nr
Match: XP_022158720.1 (alpha carbonic anhydrase 7-like [Momordica charantia])

HSP 1 Score: 398.3 bits (1022), Expect = 5.7e-107
Identity = 199/290 (68.62%), Postives = 227/290 (78.28%), Query Frame = 0


Query: 1   MENPTNKTHLLILIFAIIISCFLCLSSSDSVSAILLSDDGDEGFSYDPCSERGPKHWGDL 60
           MENPT KTHLLI    II+SC +CLSS+   SA        EGFSYDPCSERGP+HWGDL
Sbjct: 1   MENPTKKTHLLIFA-TIIVSCLVCLSSAKPNSA-------SEGFSYDPCSERGPQHWGDL 60

Query: 61  KGEWAMCKNGKMQSPVALSFWNARFTKVFGHQLTRNHWPANAVLKNNGHGVVVEWGGDAG 120
           + +WA CKNGKMQSPVALS WNA F    G QL RNHWPA A+L+NNGH VVVEW  +AG
Sbjct: 61  EEQWAKCKNGKMQSPVALSLWNATFNGYLG-QLRRNHWPAIAILENNGHQVVVEWIENAG 120

Query: 121 SIKINDIYYNLRNYHWHHPSEHTKDGKTYPLELHMVHTN---MVTGNIAVIGVLYEFGPS 180
           SIKIN + Y L++YHWHHPSEHT+DGKTYPLELHMVHTN    VT  IAVI +LY+FGP 
Sbjct: 121 SIKINGVNYYLQDYHWHHPSEHTQDGKTYPLELHMVHTNNNPNVTNKIAVISILYKFGPP 180

Query: 181 DPLLSLIEKDLKRLNIQGGEIWLGPVDPRLVW-LRSLSDRYAGYMGSLTTPPCTEGVIWT 240
           DP L LIE D+KRLNI+GGE WLG VDPRL W + S S+ Y  YMGSLTTPPCTEGVIWT
Sbjct: 181 DPFLRLIEGDIKRLNIEGGERWLGTVDPRLAWPMYSWSETYFRYMGSLTTPPCTEGVIWT 240

Query: 241 VMERVHTVSPDQLELLKQAVVEENIARPLQDVNGRPVWYYNPLL-RKSAA 286
           VM+++ TVSP Q+ELLKQAVVEE  AR LQ++N R ++Y+ PLL RKSAA
Sbjct: 241 VMKKIQTVSPYQVELLKQAVVEEKNARSLQNINRREIFYFGPLLGRKSAA 281

[Close]

BLAST of MS019618 vs. NCBI nr
Match: XP_008442729.2 (PREDICTED: alpha carbonic anhydrase 7-like [Cucumis melo])

HSP 1 Score: 334.7 bits (857), Expect = 7.7e-88
Identity = 166/289 (57.44%), Postives = 213/289 (73.70%), Query Frame = 0


Query: 1   MENPTNKTHLLILIFAIIISCFLCLSSSDSVSAILLS---DDGDEGFSYDPCSERGPKHW 60
           ME+   +TH+ +      I CF+       +S +LLS       E FSYDPCSE+GPK W
Sbjct: 1   MESLMKQTHICMK----TIYCFVL------ISLLLLSVRVKSNSEEFSYDPCSEKGPKFW 60

Query: 61  GDLKGEWAMCKNGKMQSPVALSFWNARFTKVFGHQLTRNHWPANAVLKNNGHGVVVEW-G 120
           G+L  EWA C NG +QSP+ALS W A + +  G  L RNH PANA+L+N+GH ++V+W G
Sbjct: 61  GELNIEWAKCGNGNIQSPIALSKWTADWNRGLG-CLIRNHKPANAILRNDGHEIIVDWKG 120

Query: 121 GDAGSIKINDIYYNLRNYHWHHPSEHTKDGKTYPLELHMVHTNMVTGNIAVIGVLYEFGP 180
            +AGSIKIN++ + L+NYHWHHPSEHT+DGKTYPLELHMVH N VT  IAV+GVL+E+GP
Sbjct: 121 NNAGSIKINNVDFQLQNYHWHHPSEHTQDGKTYPLELHMVHFNNVTQKIAVVGVLFEYGP 180

Query: 181 SDPLLSLIEKDLKRLNIQGGEIWLGPVDPRLVWLRSLSDRYAGYMGSLTTPPCTEGVIWT 240
            DP +S++E+D+K LNI+GGE +LG VDPR+   R    +Y  Y+GSLTTPPCTEGVIWT
Sbjct: 181 RDPFISMVERDIKYLNIEGGEEFLGKVDPRVA--RLDGRKYFRYIGSLTTPPCTEGVIWT 240

Query: 241 VMERVHTVSPDQLELLKQAVVEENIARPLQDVNGRPVWYYNPLLRKSAA 286
           VME+V TV+P Q+ELLKQA+VEE  ARPLQ +NGR V+Y++P   +S A
Sbjct: 241 VMEKVQTVAPYQVELLKQALVEEKNARPLQKINGRNVFYFDPFSWQSVA 276

[Close]

BLAST of MS019618 vs. NCBI nr
Match: KAE8651051.1 (hypothetical protein Csa_001377 [Cucumis sativus])

HSP 1 Score: 334.0 bits (855), Expect = 1.3e-87
Identity = 165/289 (57.09%), Postives = 211/289 (73.01%), Query Frame = 0


Query: 1   MENPTNKTHLLILIFAIIISCFLCLSSSDSVSAILLS---DDGDEGFSYDPCSERGPKHW 60
           MEN T +TH+ +      I CF+       +S +LLS       E FSYDPCSE+GPK W
Sbjct: 1   MENLTKQTHICMK----TICCFVL------ISLLLLSIRVKGNSEEFSYDPCSEKGPKFW 60

Query: 61  GDLKGEWAMCKNGKMQSPVALSFWNARFTKVFGHQLTRNHWPANAVLKNNGHGVVVEW-G 120
           G+L  EWA C NG +QSP+ALS W A  T+  G  L RN+ PANA+L+N+GH ++V+W G
Sbjct: 61  GELNIEWAKCGNGSIQSPIALSKWTADLTRGLG-DLRRNYRPANAILRNDGHEIIVDWTG 120

Query: 121 GDAGSIKINDIYYNLRNYHWHHPSEHTKDGKTYPLELHMVHTNMVTGNIAVIGVLYEFGP 180
            +AGSIKIN++Y+ L+NYHWHHPSEHT+DGKTYPLELHMVH N VT   AV+GVL+E+GP
Sbjct: 121 NNAGSIKINNVYFQLQNYHWHHPSEHTQDGKTYPLELHMVHFNNVTQKTAVVGVLFEYGP 180

Query: 181 SDPLLSLIEKDLKRLNIQGGEIWLGPVDPRLVWLRSLSDRYAGYMGSLTTPPCTEGVIWT 240
            DP + +IE+D+K +NI GGE +LG VDPR+   R    +Y  Y+GSLTTPPCTEGVIWT
Sbjct: 181 RDPFIYMIERDIKYINIDGGEEFLGKVDPRVA--RLDGRKYFRYIGSLTTPPCTEGVIWT 240

Query: 241 VMERVHTVSPDQLELLKQAVVEENIARPLQDVNGRPVWYYNPLLRKSAA 286
           VME V TV+P Q++LL+QA+VEE  ARPLQ +NGR V+Y++P   +S A
Sbjct: 241 VMETVQTVAPYQVKLLQQALVEEKNARPLQKINGRDVFYFDPFSWQSVA 276

[Close]

BLAST of MS019618 vs. ExPASy Swiss-Prot
Match: F4JIK2 (Alpha carbonic anhydrase 4 OS=Arabidopsis thaliana OX=3702 GN=ACA4 PE=3 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 8.9e-55
Identity = 113/260 (43.46%), Postives = 161/260 (61.92%), Query Frame = 0


Query: 21  CFLCLSSSDSVSAILLSDDGDEGFSYDPCSERGPKHWGDLKGEWAMCKNGKMQSPVALSF 80
           CF+ L S  ++S      D +  F+Y+  +E+GP+ WG +   W +C  G+ QSP+ L+ 
Sbjct: 14  CFIYL-SFPNISHAHSEVDDETPFTYEQKTEKGPEGWGKINPHWKVCNTGRYQSPIDLT- 73

Query: 81  WNARFTKVFGHQLTRNHWPANAVLKNNGHGVVVEWGGDAGSIKINDIYYNLRNYHWHHPS 140
            N R + +     TR + PA AV+ N GH ++V W GDAG + I    +NL   HWH PS
Sbjct: 74  -NERVSLIHDQAWTRQYKPAPAVITNRGHDIMVSWKGDAGKMTIRKTDFNLVQCHWHSPS 133

Query: 141 EHTKDGKTYPLELHMVHTNMVTGNIAVIGVLYEFGPSDPLLSLIEKDLKRLNIQGGEIWL 200
           EHT +G  Y LELHMVHT+   G  AVIGVLY+ G  +  L+ +   +K   +   EI L
Sbjct: 134 EHTVNGTRYDLELHMVHTS-ARGRTAVIGVLYKLGEPNEFLTKLLNGIKA--VGNKEINL 193

Query: 201 GPVDPRLVWLRSLSDRYAGYMGSLTTPPCTEGVIWTVMERVHTVSPDQLELLKQAVVE-- 260
           G +DPR +  R  + ++  Y+GSLT PPCTEGVIWTV++RV+T+S +Q+  L+QAV +  
Sbjct: 194 GMIDPREI--RFQTRKFYRYIGSLTVPPCTEGVIWTVVKRVNTISMEQITALRQAVDDGF 253

Query: 261 ENIARPLQDVNGRPVWYYNP 279
           E  +RP+QD  GR VW+Y+P
Sbjct: 254 ETNSRPVQDSKGRSVWFYDP 265

[Close]

BLAST of MS019618 vs. ExPASy Swiss-Prot
Match: A7MAQ2 (Dioscorin dioA3 OS=Dioscorea japonica OX=4673 GN=dioA3 PE=1 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 1.1e-49
Identity = 115/275 (41.82%), Postives = 161/275 (58.55%), Query Frame = 0


Query: 11  LILIFAIIISCFLCLSSSDSVSAILLSDDGDEGFSYDPCSERGPKHWGDLKGEWAMCKNG 70
           L+L+ +++ SC  CL++             ++ FSY   +  GP++WG+LK EW  C  G
Sbjct: 9   LLLLSSLLFSCLSCLTNV------------EDEFSYIEGNPNGPENWGNLKPEWETCGKG 68

Query: 71  KMQSPVALSFWNARFTKVFGHQLTRNHWPANAVLKNNGHGVVVEWGGDAGSIKINDIYYN 130
             QSP+ L      F +  G +L RN+   +A L+N+GH V+V++ G+AGS+ IN + Y 
Sbjct: 69  MEQSPIQLRDNRVIFDQTLG-KLRRNYRAVDARLRNSGHDVLVDFKGNAGSLSINRVEYQ 128

Query: 131 LRNYHWHHPSEHTKDGKTYPLELHMVHTNMVTGNIAVIGVLYEFGPSDPLLSLIEKDLKR 190
           L+  H+H PSEH  +G+ + LE  +VH +      AV+ +L+ FG +DP LS +E  +K+
Sbjct: 129 LKRIHFHSPSEHEMNGERFDLEAQLVHESQ-DQKRAVVSILFRFGRADPFLSDLEDFIKQ 188

Query: 191 L-NIQGGEIWLGPVDPRLVWLRSLSDRYAGYMGSLTTPPCTEGVIWTVMERVHTVSPDQL 250
             N Q  EI  G VDP    L+     Y  YMGS T PPCTEG+ WTVM +V TVSP Q+
Sbjct: 189 FSNSQKNEINAGVVDPN--QLQIDDSAYYRYMGSFTAPPCTEGISWTVMRKVATVSPRQV 248

Query: 251 ELLKQAVVEE--NIARPLQDVNGRPVWYYNPLLRK 283
            LLKQAV E   N ARPLQ  N R V+Y+  L  K
Sbjct: 249 LLLKQAVNENAINNARPLQPTNFRSVFYFEQLKSK 267

[Close]

BLAST of MS019618 vs. ExPASy Swiss-Prot
Match: Q8L817 (Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana OX=3702 GN=ACA7 PE=2 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 3.3e-49
Identity = 109/278 (39.21%), Postives = 167/278 (60.07%), Query Frame = 0


Query: 6   NKTHLLILIFAIIISCFLCLSSSDSVSAILLSDDGDEGFSYDPCSERGPKHWGDLKGEWA 65
           N + +  + F  + S F  +S S + S+    +D  E F+Y    E+GP+ WG+LK EW 
Sbjct: 3   NYSSISCIFFVALFSIFTIVSISSAASSHGEVEDERE-FNYKKNDEKGPERWGELKPEWE 62

Query: 66  MCKNGKMQSPVALSFWNARFTKVFGHQLTRNHWPANAVLKNNGHGVVVEWGGDAGSIKIN 125
           MC  G+MQSP+ L           G +L R++ P+NA LKN GH +++++   AG+IKIN
Sbjct: 63  MCGKGEMQSPIDLMNERVNIVSHLG-RLNRDYNPSNATLKNRGHDIMLKFEDGAGTIKIN 122

Query: 126 DIYYNLRNYHWHHPSEHTKDGKTYPLELHMVHTNMVTGNIAVIGVLYEFGPSDPLLSLIE 185
              Y L+  HWH PSEHT +G+ + LELHMVH       +AV+ VLY+ G +D  +  +E
Sbjct: 123 GFEYELQQLHWHSPSEHTINGRRFALELHMVHEGR-NRRMAVVTVLYKIGRADTFIRSLE 182

Query: 186 KDLKRL-NIQGGEIWLGPVDPRLVWLRSLSDRYAGYMGSLTTPPCTEGVIWTVMERVHTV 245
           K+L+ +  ++  E  +G +DP  + +   S +Y  Y GSLTTPPCT+ V W+V+ +V TV
Sbjct: 183 KELEGIAEMEEAEKNVGMIDPTKIKIG--SRKYYRYTGSLTTPPCTQNVTWSVVRKVRTV 242

Query: 246 SPDQLELLKQAVVEE--NIARPLQDVNGRPVWYYNPLL 281
           +  Q++LL+ AV ++  + ARP+Q  N R V  Y P++
Sbjct: 243 TRKQVKLLRVAVHDDANSNARPVQPTNKRIVHLYRPIV 275

[Close]

BLAST of MS019618 vs. ExPASy Swiss-Prot
Match: Q75N34 (Dioscorin DB3S OS=Dioscorea polystachya OX=55575 GN=DB3S PE=1 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 7.3e-49
Identity = 116/271 (42.80%), Postives = 159/271 (58.67%), Query Frame = 0


Query: 25  LSSSDSVSAILLSD-------DGDEGFSYDPCSERGPKHWGDLKGEWAMCKNGKMQSPVA 84
           +SSS  +  +LLS        + ++ FSY   +  GP++WG+LK EW  C  G  QSP+ 
Sbjct: 1   MSSSTLLHLLLLSSLLFSCLANVEDEFSYIEGNPNGPENWGNLKPEWETCGKGMEQSPIQ 60

Query: 85  LSFWNARFTKVFGHQLTRNHWPANAVLKNNGHGVVVEWGGDAGSIKINDIYYNLRNYHWH 144
           L      F +  G +L RN+   +A L+N+GH V+VE+ G+AGS+ IN + Y L+  H+H
Sbjct: 61  LRDNRVIFDQTLG-RLRRNYRAVDARLRNSGHDVLVEFKGNAGSLSINRVAYQLKRIHFH 120

Query: 145 HPSEHTKDGKTYPLELHMVHTNMVTGNIAVIGVLYEFGPSDPLLSLIEKDLKRL-NIQGG 204
            PSEH  +G+ + LE  +VH +      AV+ +L+ FG +DP LS +E  +K+  + Q  
Sbjct: 121 SPSEHEMNGERFDLEAQLVHESQ-DQKRAVVSILFIFGRADPFLSDLEDFIKQFSSSQKN 180

Query: 205 EIWLGPVDPRLVWLRSLSDRYAGYMGSLTTPPCTEGVIWTVMERVHTVSPDQLELLKQAV 264
           EI  G VDP    L+     Y  YMGS T PPCTEG+ WTVM +V TVSP Q+ LLKQAV
Sbjct: 181 EINAGVVDPN--QLQIDDSAYYRYMGSFTAPPCTEGISWTVMRKVATVSPRQVLLLKQAV 240

Query: 265 VEE--NIARPLQDVNGRPVWYYNPLLRKSAA 286
            E   N ARPLQ  N R V+Y+  L  K  A
Sbjct: 241 NENAINNARPLQPTNFRSVFYFEQLKSKVCA 267

[Close]

BLAST of MS019618 vs. ExPASy Swiss-Prot
Match: Q9FYE3 (Alpha carbonic anhydrase 3 OS=Arabidopsis thaliana OX=3702 GN=ACA3 PE=2 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 9.9e-46
Identity = 109/263 (41.44%), Postives = 153/263 (58.17%), Query Frame = 0


Query: 17  IIISCFLCLSSSDSVSAILLSDDGDEGFSYDPCSERGPKHWGDLKGEWAMCKNGKMQSPV 76
           I+   FL LSSS       L+D+ +  F Y P     P  W  +K EW +C  GK QSP+
Sbjct: 5   ILFVTFLALSSSS------LADETETEFHYKPGEIADPSKWSSIKAEWKICGTGKRQSPI 64

Query: 77  ALSFWNARFTKVFGHQLTRNHWPANAVLKNNGHGVVVEWGGDAGSIKINDIYYNLRNYHW 136
            L+   AR        L   + P  A+LKN G  + V+W  DAG I IND  Y L   HW
Sbjct: 65  NLTPKIARIVHNSTEILQTYYKPVEAILKNRGFDMKVKWEDDAGKIVINDTDYKLVQSHW 124

Query: 137 HHPSEHTKDGKTYPLELHMVHTNMVTGNIAVIGVLYEFGPSDPLLS-LIEKDLKRLNIQG 196
           H PSEH  DG+   +ELHMVH + V G++AVIGVL+  G  +  +S +++K  K  ++Q 
Sbjct: 125 HAPSEHFLDGQRLAMELHMVHKS-VEGHLAVIGVLFREGEPNAFISRIMDKIHKIADVQD 184

Query: 197 GEIWLGPVDPR-LVWLRSLSDRYAGYMGSLTTPPCTEGVIWTVMERVHTVSPDQLELL-- 256
           GE+ +G +DPR   W  +   ++  Y GSLTTPPCTE V+WT++ +V TVS +Q+++L  
Sbjct: 185 GEVSIGKIDPREFGWDLT---KFYEYRGSLTTPPCTEDVMWTIINKVGTVSREQIDVLTD 244

Query: 257 -KQAVVEENIARPLQDVNGRPVW 275
            ++   E+N ARP Q +NGR V+
Sbjct: 245 ARRGGYEKN-ARPAQPLNGRLVY 256

[Close]

BLAST of MS019618 vs. ExPASy TrEMBL
Match: A0A6J1CSG4 (alpha carbonic anhydrase 4-like OS=Momordica charantia OX=3673 GN=LOC111014349 PE=3 SV=1)

HSP 1 Score: 604.7 bits (1558), Expect = 1.9e-169
Identity = 284/287 (98.95%), Postives = 286/287 (99.65%), Query Frame = 0


Query: 1   MENPTNKTHLLILIFAIIISCFLCLSSSDSVSAILLSDDGDEGFSYDPCSERGPKHWGDL 60
           MENPTNKTHLLILIFAIIISCFLCLSSSDSVSAILLSDDGDEGFSYDPCSERGPKHWGDL
Sbjct: 1   MENPTNKTHLLILIFAIIISCFLCLSSSDSVSAILLSDDGDEGFSYDPCSERGPKHWGDL 60

Query: 61  KGEWAMCKNGKMQSPVALSFWNARFTKVFGHQLTRNHWPANAVLKNNGHGVVVEWGGDAG 120
           K EWAMCKNGKMQSPVALSFWNARFTKVFGHQLTRNHWPANAVLKNNGHGVVVEWGGDAG
Sbjct: 61  KEEWAMCKNGKMQSPVALSFWNARFTKVFGHQLTRNHWPANAVLKNNGHGVVVEWGGDAG 120

Query: 121 SIKINDIYYNLRNYHWHHPSEHTKDGKTYPLELHMVHTNMVTGNIAVIGVLYEFGPSDPL 180
           SIKINDIYYNLRNYHWHHPSEHTKDGKTYPLELHMVHTNMVTGNIAVIGVLYEFGPSDPL
Sbjct: 121 SIKINDIYYNLRNYHWHHPSEHTKDGKTYPLELHMVHTNMVTGNIAVIGVLYEFGPSDPL 180

Query: 181 LSLIEKDLKRLNIQGGEIWLGPVDPRLVWLRSLSDRYAGYMGSLTTPPCTEGVIWTVMER 240
           LSLIEKDLKRLNI+GGEIWLGPVDPRLVWLRSLSD+YAGYMGSLTTPPCTEGVIWTVMER
Sbjct: 181 LSLIEKDLKRLNIEGGEIWLGPVDPRLVWLRSLSDKYAGYMGSLTTPPCTEGVIWTVMER 240

Query: 241 VHTVSPDQLELLKQAVVEENIARPLQDVNGRPVWYYNPLLRKSAADQ 288
           VHTVSPDQLELLKQAVVEENIARPLQDVNGRPVWYYNPLLRKSAADQ
Sbjct: 241 VHTVSPDQLELLKQAVVEENIARPLQDVNGRPVWYYNPLLRKSAADQ 287

[Close]

BLAST of MS019618 vs. ExPASy TrEMBL
Match: A0A6J1CSH0 (alpha carbonic anhydrase 4-like OS=Momordica charantia OX=3673 GN=LOC111014353 PE=3 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 5.7e-145
Identity = 250/286 (87.41%), Postives = 262/286 (91.61%), Query Frame = 0


Query: 1   MENPTNKTHLLILIFAIIISCFLCLSSSDSVSAILLSDDGDEGFSYDPCSERGPKHWGDL 60
           MENPTNKTH+LILIFAI ISCFLCLSSS SVSAI LS+DGDEGFSYDPCSERGPKHWG+L
Sbjct: 1   MENPTNKTHILILIFAITISCFLCLSSSVSVSAI-LSNDGDEGFSYDPCSERGPKHWGNL 60

Query: 61  KGEWAMCKNGKMQSPVALSFWNARFTKVFGHQLTRNHWPANAVLKNNGHGVVVEWGGDAG 120
           K EWAMCKNGKMQSP+ALSFWNARFT VFGH++TRNHWPANAVLKNNGH VVVEW GDAG
Sbjct: 61  KEEWAMCKNGKMQSPIALSFWNARFTNVFGHRITRNHWPANAVLKNNGHEVVVEWKGDAG 120

Query: 121 SIKINDIYYNLRNYHWHHPSEHTKDGKTYPLELHMVHTNMVTGNIAVIGVLYEFGPSDPL 180
           SIKINDI Y+L+NYHWHHPSEH KDGKTYPLELHMVHTNMVTGN AVIGVLYEFGPSDPL
Sbjct: 121 SIKINDIDYHLKNYHWHHPSEHIKDGKTYPLELHMVHTNMVTGNKAVIGVLYEFGPSDPL 180

Query: 181 LSLIEKDLKRLNIQGGEIWLGPVDPRLVWLRSLSDRYAGYMGSLTTPPCTEGVIWTVMER 240
           LSLIEKDLK LNI+GGE W G VDPRLVWL S S+ YAGYMGSLTTPPCTEGVIWTVMER
Sbjct: 181 LSLIEKDLKHLNIEGGETWFGEVDPRLVWLYSSSEGYAGYMGSLTTPPCTEGVIWTVMER 240

Query: 241 VHTVSPDQLELLKQAVVEENIARPLQDVNGRPVWYYNPLLRKSAAD 287
           VHTVSPDQLELLK AVVEEN ARPLQDV G  V YY+P LRKSAA+
Sbjct: 241 VHTVSPDQLELLKLAVVEENTARPLQDVKGPRVGYYDPSLRKSAAE 285

[Close]

BLAST of MS019618 vs. ExPASy TrEMBL
Match: A0A6J1DWL5 (alpha carbonic anhydrase 7-like OS=Momordica charantia OX=3673 GN=LOC111025178 PE=3 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 2.8e-107
Identity = 199/290 (68.62%), Postives = 227/290 (78.28%), Query Frame = 0


Query: 1   MENPTNKTHLLILIFAIIISCFLCLSSSDSVSAILLSDDGDEGFSYDPCSERGPKHWGDL 60
           MENPT KTHLLI    II+SC +CLSS+   SA        EGFSYDPCSERGP+HWGDL
Sbjct: 1   MENPTKKTHLLIFA-TIIVSCLVCLSSAKPNSA-------SEGFSYDPCSERGPQHWGDL 60

Query: 61  KGEWAMCKNGKMQSPVALSFWNARFTKVFGHQLTRNHWPANAVLKNNGHGVVVEWGGDAG 120
           + +WA CKNGKMQSPVALS WNA F    G QL RNHWPA A+L+NNGH VVVEW  +AG
Sbjct: 61  EEQWAKCKNGKMQSPVALSLWNATFNGYLG-QLRRNHWPAIAILENNGHQVVVEWIENAG 120

Query: 121 SIKINDIYYNLRNYHWHHPSEHTKDGKTYPLELHMVHTN---MVTGNIAVIGVLYEFGPS 180
           SIKIN + Y L++YHWHHPSEHT+DGKTYPLELHMVHTN    VT  IAVI +LY+FGP 
Sbjct: 121 SIKINGVNYYLQDYHWHHPSEHTQDGKTYPLELHMVHTNNNPNVTNKIAVISILYKFGPP 180

Query: 181 DPLLSLIEKDLKRLNIQGGEIWLGPVDPRLVW-LRSLSDRYAGYMGSLTTPPCTEGVIWT 240
           DP L LIE D+KRLNI+GGE WLG VDPRL W + S S+ Y  YMGSLTTPPCTEGVIWT
Sbjct: 181 DPFLRLIEGDIKRLNIEGGERWLGTVDPRLAWPMYSWSETYFRYMGSLTTPPCTEGVIWT 240

Query: 241 VMERVHTVSPDQLELLKQAVVEENIARPLQDVNGRPVWYYNPLL-RKSAA 286
           VM+++ TVSP Q+ELLKQAVVEE  AR LQ++N R ++Y+ PLL RKSAA
Sbjct: 241 VMKKIQTVSPYQVELLKQAVVEEKNARSLQNINRREIFYFGPLLGRKSAA 281

[Close]

BLAST of MS019618 vs. ExPASy TrEMBL
Match: A0A1S3B753 (alpha carbonic anhydrase 7-like OS=Cucumis melo OX=3656 GN=LOC103486518 PE=3 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 3.7e-88
Identity = 166/289 (57.44%), Postives = 213/289 (73.70%), Query Frame = 0


Query: 1   MENPTNKTHLLILIFAIIISCFLCLSSSDSVSAILLS---DDGDEGFSYDPCSERGPKHW 60
           ME+   +TH+ +      I CF+       +S +LLS       E FSYDPCSE+GPK W
Sbjct: 1   MESLMKQTHICMK----TIYCFVL------ISLLLLSVRVKSNSEEFSYDPCSEKGPKFW 60

Query: 61  GDLKGEWAMCKNGKMQSPVALSFWNARFTKVFGHQLTRNHWPANAVLKNNGHGVVVEW-G 120
           G+L  EWA C NG +QSP+ALS W A + +  G  L RNH PANA+L+N+GH ++V+W G
Sbjct: 61  GELNIEWAKCGNGNIQSPIALSKWTADWNRGLG-CLIRNHKPANAILRNDGHEIIVDWKG 120

Query: 121 GDAGSIKINDIYYNLRNYHWHHPSEHTKDGKTYPLELHMVHTNMVTGNIAVIGVLYEFGP 180
            +AGSIKIN++ + L+NYHWHHPSEHT+DGKTYPLELHMVH N VT  IAV+GVL+E+GP
Sbjct: 121 NNAGSIKINNVDFQLQNYHWHHPSEHTQDGKTYPLELHMVHFNNVTQKIAVVGVLFEYGP 180

Query: 181 SDPLLSLIEKDLKRLNIQGGEIWLGPVDPRLVWLRSLSDRYAGYMGSLTTPPCTEGVIWT 240
            DP +S++E+D+K LNI+GGE +LG VDPR+   R    +Y  Y+GSLTTPPCTEGVIWT
Sbjct: 181 RDPFISMVERDIKYLNIEGGEEFLGKVDPRVA--RLDGRKYFRYIGSLTTPPCTEGVIWT 240

Query: 241 VMERVHTVSPDQLELLKQAVVEENIARPLQDVNGRPVWYYNPLLRKSAA 286
           VME+V TV+P Q+ELLKQA+VEE  ARPLQ +NGR V+Y++P   +S A
Sbjct: 241 VMEKVQTVAPYQVELLKQALVEEKNARPLQKINGRNVFYFDPFSWQSVA 276

[Close]

BLAST of MS019618 vs. ExPASy TrEMBL
Match: A0A5A7TPG5 (Alpha carbonic anhydrase 7-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002900 PE=3 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 1.4e-87
Identity = 156/245 (63.67%), Postives = 195/245 (79.59%), Query Frame = 0


Query: 42  EGFSYDPCSERGPKHWGDLKGEWAMCKNGKMQSPVALSFWNARFTKVFGHQLTRNHWPAN 101
           E FSYDPCSE+GPK WG+L  EWA C NG +QSP+ALS W A + +  G  L RNH PAN
Sbjct: 8   EEFSYDPCSEKGPKFWGELNIEWAKCGNGNIQSPIALSKWTADWNRGLG-CLIRNHKPAN 67

Query: 102 AVLKNNGHGVVVEW-GGDAGSIKINDIYYNLRNYHWHHPSEHTKDGKTYPLELHMVHTNM 161
           A+L+N+GH ++V+W G +AGSIKIN++ + L+NYHWHHPSEHT+DGKTYPLELHMVH N 
Sbjct: 68  AILRNDGHEIIVDWKGNNAGSIKINNVDFQLQNYHWHHPSEHTQDGKTYPLELHMVHFNN 127

Query: 162 VTGNIAVIGVLYEFGPSDPLLSLIEKDLKRLNIQGGEIWLGPVDPRLVWLRSLSDRYAGY 221
           VT  IAV+GVL+E+GP DP +S+IE+D+K LNI+GGE +LG VDPR+   R    +Y  Y
Sbjct: 128 VTQKIAVVGVLFEYGPRDPFISMIERDIKYLNIEGGEEFLGKVDPRVA--RLDGRKYFRY 187

Query: 222 MGSLTTPPCTEGVIWTVMERVHTVSPDQLELLKQAVVEENIARPLQDVNGRPVWYYNPLL 281
           +GSLTTPPCTEGVIWTVME+V TV+P Q+ELLKQA+VEE  ARPLQ +NGR V+Y++P  
Sbjct: 188 IGSLTTPPCTEGVIWTVMEKVQTVAPYQVELLKQALVEEKNARPLQKINGRNVFYFDPFS 247

Query: 282 RKSAA 286
            +S A
Sbjct: 248 WQSVA 249

[Close]

BLAST of MS019618 vs. TAIR 10
Match: AT4G20990.1 (alpha carbonic anhydrase 4 )

HSP 1 Score: 215.3 bits (547), Expect = 6.4e-56
Identity = 113/260 (43.46%), Postives = 161/260 (61.92%), Query Frame = 0


Query: 21  CFLCLSSSDSVSAILLSDDGDEGFSYDPCSERGPKHWGDLKGEWAMCKNGKMQSPVALSF 80
           CF+ L S  ++S      D +  F+Y+  +E+GP+ WG +   W +C  G+ QSP+ L+ 
Sbjct: 14  CFIYL-SFPNISHAHSEVDDETPFTYEQKTEKGPEGWGKINPHWKVCNTGRYQSPIDLT- 73

Query: 81  WNARFTKVFGHQLTRNHWPANAVLKNNGHGVVVEWGGDAGSIKINDIYYNLRNYHWHHPS 140
            N R + +     TR + PA AV+ N GH ++V W GDAG + I    +NL   HWH PS
Sbjct: 74  -NERVSLIHDQAWTRQYKPAPAVITNRGHDIMVSWKGDAGKMTIRKTDFNLVQCHWHSPS 133

Query: 141 EHTKDGKTYPLELHMVHTNMVTGNIAVIGVLYEFGPSDPLLSLIEKDLKRLNIQGGEIWL 200
           EHT +G  Y LELHMVHT+   G  AVIGVLY+ G  +  L+ +   +K   +   EI L
Sbjct: 134 EHTVNGTRYDLELHMVHTS-ARGRTAVIGVLYKLGEPNEFLTKLLNGIKA--VGNKEINL 193

Query: 201 GPVDPRLVWLRSLSDRYAGYMGSLTTPPCTEGVIWTVMERVHTVSPDQLELLKQAVVE-- 260
           G +DPR +  R  + ++  Y+GSLT PPCTEGVIWTV++RV+T+S +Q+  L+QAV +  
Sbjct: 194 GMIDPREI--RFQTRKFYRYIGSLTVPPCTEGVIWTVVKRVNTISMEQITALRQAVDDGF 253

Query: 261 ENIARPLQDVNGRPVWYYNP 279
           E  +RP+QD  GR VW+Y+P
Sbjct: 254 ETNSRPVQDSKGRSVWFYDP 265

[Close]

BLAST of MS019618 vs. TAIR 10
Match: AT1G08080.1 (alpha carbonic anhydrase 7 )

HSP 1 Score: 196.8 bits (499), Expect = 2.3e-50
Identity = 109/278 (39.21%), Postives = 167/278 (60.07%), Query Frame = 0


Query: 6   NKTHLLILIFAIIISCFLCLSSSDSVSAILLSDDGDEGFSYDPCSERGPKHWGDLKGEWA 65
           N + +  + F  + S F  +S S + S+    +D  E F+Y    E+GP+ WG+LK EW 
Sbjct: 3   NYSSISCIFFVALFSIFTIVSISSAASSHGEVEDERE-FNYKKNDEKGPERWGELKPEWE 62

Query: 66  MCKNGKMQSPVALSFWNARFTKVFGHQLTRNHWPANAVLKNNGHGVVVEWGGDAGSIKIN 125
           MC  G+MQSP+ L           G +L R++ P+NA LKN GH +++++   AG+IKIN
Sbjct: 63  MCGKGEMQSPIDLMNERVNIVSHLG-RLNRDYNPSNATLKNRGHDIMLKFEDGAGTIKIN 122

Query: 126 DIYYNLRNYHWHHPSEHTKDGKTYPLELHMVHTNMVTGNIAVIGVLYEFGPSDPLLSLIE 185
              Y L+  HWH PSEHT +G+ + LELHMVH       +AV+ VLY+ G +D  +  +E
Sbjct: 123 GFEYELQQLHWHSPSEHTINGRRFALELHMVHEGR-NRRMAVVTVLYKIGRADTFIRSLE 182

Query: 186 KDLKRL-NIQGGEIWLGPVDPRLVWLRSLSDRYAGYMGSLTTPPCTEGVIWTVMERVHTV 245
           K+L+ +  ++  E  +G +DP  + +   S +Y  Y GSLTTPPCT+ V W+V+ +V TV
Sbjct: 183 KELEGIAEMEEAEKNVGMIDPTKIKIG--SRKYYRYTGSLTTPPCTQNVTWSVVRKVRTV 242

Query: 246 SPDQLELLKQAVVEE--NIARPLQDVNGRPVWYYNPLL 281
           +  Q++LL+ AV ++  + ARP+Q  N R V  Y P++
Sbjct: 243 TRKQVKLLRVAVHDDANSNARPVQPTNKRIVHLYRPIV 275

[Close]

BLAST of MS019618 vs. TAIR 10
Match: AT5G04180.1 (alpha carbonic anhydrase 3 )

HSP 1 Score: 185.3 bits (469), Expect = 7.0e-47
Identity = 109/263 (41.44%), Postives = 153/263 (58.17%), Query Frame = 0


Query: 17  IIISCFLCLSSSDSVSAILLSDDGDEGFSYDPCSERGPKHWGDLKGEWAMCKNGKMQSPV 76
           I+   FL LSSS       L+D+ +  F Y P     P  W  +K EW +C  GK QSP+
Sbjct: 5   ILFVTFLALSSSS------LADETETEFHYKPGEIADPSKWSSIKAEWKICGTGKRQSPI 64

Query: 77  ALSFWNARFTKVFGHQLTRNHWPANAVLKNNGHGVVVEWGGDAGSIKINDIYYNLRNYHW 136
            L+   AR        L   + P  A+LKN G  + V+W  DAG I IND  Y L   HW
Sbjct: 65  NLTPKIARIVHNSTEILQTYYKPVEAILKNRGFDMKVKWEDDAGKIVINDTDYKLVQSHW 124

Query: 137 HHPSEHTKDGKTYPLELHMVHTNMVTGNIAVIGVLYEFGPSDPLLS-LIEKDLKRLNIQG 196
           H PSEH  DG+   +ELHMVH + V G++AVIGVL+  G  +  +S +++K  K  ++Q 
Sbjct: 125 HAPSEHFLDGQRLAMELHMVHKS-VEGHLAVIGVLFREGEPNAFISRIMDKIHKIADVQD 184

Query: 197 GEIWLGPVDPR-LVWLRSLSDRYAGYMGSLTTPPCTEGVIWTVMERVHTVSPDQLELL-- 256
           GE+ +G +DPR   W  +   ++  Y GSLTTPPCTE V+WT++ +V TVS +Q+++L  
Sbjct: 185 GEVSIGKIDPREFGWDLT---KFYEYRGSLTTPPCTEDVMWTIINKVGTVSREQIDVLTD 244

Query: 257 -KQAVVEENIARPLQDVNGRPVW 275
            ++   E+N ARP Q +NGR V+
Sbjct: 245 ARRGGYEKN-ARPAQPLNGRLVY 256

[Close]

BLAST of MS019618 vs. TAIR 10
Match: AT4G21000.1 (alpha carbonic anhydrase 6 )

HSP 1 Score: 178.3 bits (451), Expect = 8.6e-45
Identity = 90/198 (45.45%), Postives = 126/198 (63.64%), Query Frame = 0


Query: 44  FSYDPCSERGPKHWGDLKGEWAMCKNGKMQSPVALSFWNARFTKVFGHQLTRNHWPANAV 103
           F+Y   +E+GP  WG L  +W +C  GK+QSP+ L+  + R + +    L++++ PA+AV
Sbjct: 37  FTYKQKTEKGPAEWGKLDPQWKVCSTGKIQSPIDLT--DERVSLIHDQALSKHYKPASAV 96

Query: 104 LKNNGHGVVVEWGGDAGSIKINDIYYNLRNYHWHHPSEHTKDGKTYPLELHMVHTNMVTG 163
           +++ GH V+V W GD G I I+   Y L   HWH PSEHT +G +Y LELHMVHT+  +G
Sbjct: 97  IQSRGHDVMVSWKGDGGKITIHQTDYKLVQCHWHSPSEHTINGTSYDLELHMVHTS-ASG 156

Query: 164 NIAVIGVLYEFGPSDPLLSLIEKDLKRLNIQGGEIWLGPVDPRLVWLRSLSDRYAGYMGS 223
              V+GVLY+ G  D  L+ I   +K   +   EI LG VDPR +  R  ++ +  Y+GS
Sbjct: 157 KTTVVGVLYKLGEPDEFLTKILNGIK--GVGKKEIDLGIVDPRDI--RFETNNFYRYIGS 216

Query: 224 LTTPPCTEGVIWTVMERV 242
           LT PPCTEGVIWTV +RV
Sbjct: 217 LTIPPCTEGVIWTVQKRV 227

[Close]

BLAST of MS019618 vs. TAIR 10
Match: AT2G28210.1 (alpha carbonic anhydrase 2 )

HSP 1 Score: 176.4 bits (446), Expect = 3.3e-44
Identity = 90/207 (43.48%), Postives = 130/207 (62.80%), Query Frame = 0


Query: 39  DGDEGFSYDPCSERGPKHWGDLKGEWAMCKNGKMQSPVALSFWNARFTKVFGHQLTRNHW 98
           + +  FSY+   E GP  WG L+ EW MC  G+MQSP+ L     R       +LTR++ 
Sbjct: 6   EDEHEFSYEWNQENGPAKWGKLRPEWKMCGKGEMQSPIDLMNKRVRLVTHL-KKLTRHYK 65

Query: 99  PANAVLKNNGHGVVVEWGGD-AGSIKINDIYYNLRNYHWHHPSEHTKDGKTYPLELHMVH 158
           P NA LKN GH +++++G + +GSI +N   Y L   HWH PSEHT +G+ + LELHMVH
Sbjct: 66  PCNATLKNRGHDMMLKFGEEGSGSITVNGTEYKLLQLHWHSPSEHTMNGRRFALELHMVH 125

Query: 159 TNMVTGNIAVIGVLYEFGPSDPLLSLIEKDLKRLNIQG-GEIWLGPVDPRLVWLRSLSDR 218
            N + G++AV+ VLY+ G  D  L L+E  L  +  Q   E ++  +DPR + +   S +
Sbjct: 126 EN-INGSLAVVTVLYKIGRPDSFLGLLENKLSAITDQNEAEKYVDVIDPRDIKIG--SRK 185

Query: 219 YAGYMGSLTTPPCTEGVIWTVMERVHT 244
           +  Y+GSLTTPPCT+ VIWTV+++V+T
Sbjct: 186 FYRYIGSLTTPPCTQNVIWTVVKKVNT 208

The following BLAST results are available for this feature:

BLAST of MS019618 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022144730.1	4.0e-169	98.95	alpha carbonic anhydrase 4-like [Momordica charantia]	[more]
XP_022144735.1	1.2e-144	87.41	alpha carbonic anhydrase 4-like [Momordica charantia]	[more]
XP_022158720.1	5.7e-107	68.62	alpha carbonic anhydrase 7-like [Momordica charantia]	[more]
XP_008442729.2	7.7e-88	57.44	PREDICTED: alpha carbonic anhydrase 7-like [Cucumis melo]	[more]
KAE8651051.1	1.3e-87	57.09	hypothetical protein Csa_001377 [Cucumis sativus]	[more]

BLAST of MS019618 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JIK2	8.9e-55	43.46	Alpha carbonic anhydrase 4 OS=Arabidopsis thaliana OX=3702 GN=ACA4 PE=3 SV=1	[more]
A7MAQ2	1.1e-49	41.82	Dioscorin dioA3 OS=Dioscorea japonica OX=4673 GN=dioA3 PE=1 SV=1	[more]
Q8L817	3.3e-49	39.21	Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana OX=3702 GN=ACA7 PE=2 SV=1	[more]
Q75N34	7.3e-49	42.80	Dioscorin DB3S OS=Dioscorea polystachya OX=55575 GN=DB3S PE=1 SV=1	[more]
Q9FYE3	9.9e-46	41.44	Alpha carbonic anhydrase 3 OS=Arabidopsis thaliana OX=3702 GN=ACA3 PE=2 SV=1	[more]

BLAST of MS019618 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CSG4	1.9e-169	98.95	alpha carbonic anhydrase 4-like OS=Momordica charantia OX=3673 GN=LOC111014349 P...	[more]
A0A6J1CSH0	5.7e-145	87.41	alpha carbonic anhydrase 4-like OS=Momordica charantia OX=3673 GN=LOC111014353 P...	[more]
A0A6J1DWL5	2.8e-107	68.62	alpha carbonic anhydrase 7-like OS=Momordica charantia OX=3673 GN=LOC111025178 P...	[more]
A0A1S3B753	3.7e-88	57.44	alpha carbonic anhydrase 7-like OS=Cucumis melo OX=3656 GN=LOC103486518 PE=3 SV=...	[more]
A0A5A7TPG5	1.4e-87	63.67	Alpha carbonic anhydrase 7-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]

BLAST of MS019618 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G20990.1	6.4e-56	43.46	alpha carbonic anhydrase 4	[more]
AT1G08080.1	2.3e-50	39.21	alpha carbonic anhydrase 7	[more]
AT5G04180.1	7.0e-47	41.44	alpha carbonic anhydrase 3	[more]
AT4G21000.1	8.6e-45	45.45	alpha carbonic anhydrase 6	[more]
AT2G28210.1	3.3e-44	43.48	alpha carbonic anhydrase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001148	Alpha carbonic anhydrase domain	SMART	SM01057	Carb_anhydrase_2a	coord: 44..277 e-value: 6.0E-54 score: 195.2
IPR001148	Alpha carbonic anhydrase domain	PFAM	PF00194	Carb_anhydrase	coord: 50..275 e-value: 6.6E-36 score: 124.2
IPR001148	Alpha carbonic anhydrase domain	PROSITE	PS51144	ALPHA_CA_2	coord: 42..277 score: 41.939766
IPR036398	Alpha carbonic anhydrase domain superfamily	GENE3D	3.10.200.10	Alpha carbonic anhydrase	coord: 41..283 e-value: 1.1E-64 score: 220.2
IPR036398	Alpha carbonic anhydrase domain superfamily	SUPERFAMILY	51069	Carbonic anhydrase	coord: 44..274
None	No IPR available	PANTHER	PTHR18952:SF201	ALPHA CARBONIC ANHYDRASE 3	coord: 15..278
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..21 score: 5.0
IPR023561	Carbonic anhydrase, alpha-class	PANTHER	PTHR18952	CARBONIC ANHYDRASE	coord: 15..278
IPR041891	Carbonic anhydrase, prokaryotic-like	CDD	cd03124	alpha_CA_prokaryotic_like	coord: 53..275 e-value: 1.13878E-83 score: 248.726

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS019618.1	MS019618.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004089	carbonate dehydratase activity
molecular_function	GO:0008270	zinc ion binding