MS019614 (gene) Bitter gourd (TR) v1

Overview


Name	MS019614
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	ANK_REP_REGION domain-containing protein
Location	scaffold729: 1014754 .. 1016711 (-)
RNA-Seq Expression	MS019614
Synteny	MS019614

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGTTCCCGGCAGGCGCAACGGCATGGTAGACGAAGACGATTACGAGGAAGATAATGCCCTCTTTGAAGAGGATGGTGTCATGGAATTCGATTCTGATACGCCACCACACCTCCGCCCCCTAGCTACCGCCGCCCAGCTCGGCGACGTTAATGGCCTGCGCTCCGCTCTAGGTACAGCTTATTGCATTCGCTCTCGCACTGCGAAATTTTCTGTCAATCGTACTGTAGGGTCTTCACTATCCTGATTTGGTCGAAAAGGTCCTTTTCAGAAAATTTGATGTGAACACAATTCCGATTGACCTTTTCCTCACTTTTTGAAATTTCAATTTGTAATGTGCTTATACATGTTGTCATAGTTCAATTACTATGCTCTGCAAACTGCGAAGATGGTGTTTATCGTTGTCTACTGAGTCCACCATCCTCATTGGAAAGATATGGTAGTCTAAAGGAATGGTGGTTAGTTTTCTTGATGAGCGAGGGTTGTCTAAATGGGAACTGCTCTTTTGGTGTATGATAATTTAAATCTAAGTTTATGTTTTTAGCTCTGTGTTTATTTTGATGCAGAATCAGTTGCATGAAAAGGAAACTTGTAATATTTTTCATTGAGTGGTTTGCATATATCATTAATGTCATTTACATCCATTCATTGGACTGATTCGAGTGTAAGATATGTGAATCAGGCCTCTGGTTTCAACTTTGTTGAAGCATTTTTCTTGTTTTGAGTTAATTGTTAATGTTTTATTCAAATGTTTTCGGATTTCCTTCAATTCAGATAACTTGACAATGGGAAGTATAGATGATGCTGTGGAAGATGGGGATACAGCTCTCCACCTTACTTGTCTCTACGGCCATCTGCCATGTGTCCAGGTTGGCGACCTATTCTATGCATACTTTGTATATTTTATTTTTGAATTTTTTATGATTTATTATGACTTCTTTTAAAATAAATTAATCTTAATATTAGTTACTGCTGGAAAGAGGGGCAAACTTGGAGGTTGAAGATGAGGATGGTGCCATTCCTTTGCATGATGCTTGTGCAGGGGGTGGGTTGGCTATTATGATTTATGAATAATTTGCCCTTTTTCAGCACCCCATTGTAACATATTATTCTCTTCCAAATGTTATTTTCATTTTCTTTTCTTCTCCTCCCATGATTTGTGCAGGTTTTTTAGAGATTGTTCAACTCCTAATTAACAGTGCTAACAACACAGACTGCCTGAAGAGGATGCTAGAATCAGTCGATGCAGAAGGTGACACTGTAAGTATCTGGTTATAATCAGTATCACTACTTAATTTGTATCAAAATCTTCAGCCAAATACTTACAACAACCCAACAATGTTGTTCCTTCTTCTTGATGCTATAAAATATAGAAAAGACTTCTATTTTCTTTGGCTATATGGTAAAAGAAAAGTACTCCGGAGCATCATTATTCTAGAAAAATTTTTCATGGTGCAGTTGCATTGGCTTCTTCAATAATAGGAACAATAGGCTTTTTAAAGTGTACACAATCTCTTTACACATCATGCATGGTAGGGTATGAAATCTATTGGCTTGCGAAATAGAAAATTACCTTTTTATACAAACTTTTCATCACTCCTAAAAAATCACTTAGTAAAAAATCACCGTTTGTTTTACGGTCACGAATAGCAATCAGTTTCAATAAGCTCATTCCTCTAACATTATCTGTACAGCCTCTTCATCATGCCGCAAGAGGCGAGCATGCTGCAGTTGTAAGGTTACTGCTAGCTTCAGGGGCATCTCCCACCAAGGAAAATACATACGGGAAGGTTTGTATTCGAGTGGATATTGAAACTTCCATCTCCCTTTATATGGCAGCACCCATTTTCTAAGTATTGCTTCTGTTTTCAGGCCCCGACTGAACTTGCCGATCCTGGGACGGAGGCCAGGACGATTTTGGAGGCTGCCTCGGCCCAATTCGCGATGGGGTGTCAG

mRNA sequence

ATGGCGGTTCCCGGCAGGCGCAACGGCATGGTAGACGAAGACGATTACGAGGAAGATAATGCCCTCTTTGAAGAGGATGGTGTCATGGAATTCGATTCTGATACGCCACCACACCTCCGCCCCCTAGCTACCGCCGCCCAGCTCGGCGACGTTAATGGCCTGCGCTCCGCTCTAGATAACTTGACAATGGGAAGTATAGATGATGCTGTGGAAGATGGGGATACAGCTCTCCACCTTACTTGTCTCTACGGCCATCTGCCATGTGTCCAGTTACTGCTGGAAAGAGGGGCAAACTTGGAGGTTGAAGATGAGGATGGTGCCATTCCTTTGCATGATGCTTGTGCAGGGGGTTTTTTAGAGATTGTTCAACTCCTAATTAACAGTGCTAACAACACAGACTGCCTGAAGAGGATGCTAGAATCAGTCGATGCAGAAGGTGACACTCCTCTTCATCATGCCGCAAGAGGCGAGCATGCTGCAGTTGTAAGGTTACTGCTAGCTTCAGGGGCATCTCCCACCAAGGAAAATACATACGGGAAGGCCCCGACTGAACTTGCCGATCCTGGGACGGAGGCCAGGACGATTTTGGAGGCTGCCTCGGCCCAATTCGCGATGGGGTGTCAG

Coding sequence (CDS)

ATGGCGGTTCCCGGCAGGCGCAACGGCATGGTAGACGAAGACGATTACGAGGAAGATAATGCCCTCTTTGAAGAGGATGGTGTCATGGAATTCGATTCTGATACGCCACCACACCTCCGCCCCCTAGCTACCGCCGCCCAGCTCGGCGACGTTAATGGCCTGCGCTCCGCTCTAGATAACTTGACAATGGGAAGTATAGATGATGCTGTGGAAGATGGGGATACAGCTCTCCACCTTACTTGTCTCTACGGCCATCTGCCATGTGTCCAGTTACTGCTGGAAAGAGGGGCAAACTTGGAGGTTGAAGATGAGGATGGTGCCATTCCTTTGCATGATGCTTGTGCAGGGGGTTTTTTAGAGATTGTTCAACTCCTAATTAACAGTGCTAACAACACAGACTGCCTGAAGAGGATGCTAGAATCAGTCGATGCAGAAGGTGACACTCCTCTTCATCATGCCGCAAGAGGCGAGCATGCTGCAGTTGTAAGGTTACTGCTAGCTTCAGGGGCATCTCCCACCAAGGAAAATACATACGGGAAGGCCCCGACTGAACTTGCCGATCCTGGGACGGAGGCCAGGACGATTTTGGAGGCTGCCTCGGCCCAATTCGCGATGGGGTGTCAG

Protein sequence

MAVPGRRNGMVDEDDYEEDNALFEEDGVMEFDSDTPPHLRPLATAAQLGDVNGLRSALDNLTMGSIDDAVEDGDTALHLTCLYGHLPCVQLLLERGANLEVEDEDGAIPLHDACAGGFLEIVQLLINSANNTDCLKRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENTYGKAPTELADPGTEARTILEAASAQFAMGCQ

Homology

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BLAST of MS019614 vs. NCBI nr
Match: XP_022144606.1 (tankyrase-2 [Momordica charantia])

HSP 1 Score: 419.1 bits (1076), Expect = 2.3e-113
Identity = 208/208 (100.00%), Postives = 208/208 (100.00%), Query Frame = 0


Query: 1   MAVPGRRNGMVDEDDYEEDNALFEEDGVMEFDSDTPPHLRPLATAAQLGDVNGLRSALDN 60
           MAVPGRRNGMVDEDDYEEDNALFEEDGVMEFDSDTPPHLRPLATAAQLGDVNGLRSALDN
Sbjct: 1   MAVPGRRNGMVDEDDYEEDNALFEEDGVMEFDSDTPPHLRPLATAAQLGDVNGLRSALDN 60

Query: 61  LTMGSIDDAVEDGDTALHLTCLYGHLPCVQLLLERGANLEVEDEDGAIPLHDACAGGFLE 120
           LTMGSIDDAVEDGDTALHLTCLYGHLPCVQLLLERGANLEVEDEDGAIPLHDACAGGFLE
Sbjct: 61  LTMGSIDDAVEDGDTALHLTCLYGHLPCVQLLLERGANLEVEDEDGAIPLHDACAGGFLE 120

Query: 121 IVQLLINSANNTDCLKRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENTYGK 180
           IVQLLINSANNTDCLKRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENTYGK
Sbjct: 121 IVQLLINSANNTDCLKRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENTYGK 180

Query: 181 APTELADPGTEARTILEAASAQFAMGCQ 209
           APTELADPGTEARTILEAASAQFAMGCQ
Sbjct: 181 APTELADPGTEARTILEAASAQFAMGCQ 208

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BLAST of MS019614 vs. NCBI nr
Match: XP_038905294.1 (poly [ADP-ribose] polymerase tankyrase [Benincasa hispida])

HSP 1 Score: 386.0 bits (990), Expect = 2.1e-103
Identity = 190/208 (91.35%), Postives = 201/208 (96.63%), Query Frame = 0


Query: 1   MAVPGRRNGMVDEDDYEEDNALFEEDGVMEFDSDTPPHLRPLATAAQLGDVNGLRSALDN 60
           MAVPGRRNGMVDEDDYEEDNALFEEDGV+EFDSDTPPHLR LATAAQLGDV+GLR+ALDN
Sbjct: 1   MAVPGRRNGMVDEDDYEEDNALFEEDGVIEFDSDTPPHLRALATAAQLGDVDGLRTALDN 60

Query: 61  LTMGSIDDAVEDGDTALHLTCLYGHLPCVQLLLERGANLEVEDEDGAIPLHDACAGGFLE 120
           LT+GSI+DAVEDGDTALHLTCLYGHLPCVQLLLERGA LEV+DEDGAIPLHDACAGGF+E
Sbjct: 61  LTVGSINDAVEDGDTALHLTCLYGHLPCVQLLLERGAALEVQDEDGAIPLHDACAGGFVE 120

Query: 121 IVQLLINSANNTDCLKRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENTYGK 180
           IVQLLINSAN+T+C+KRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKEN YGK
Sbjct: 121 IVQLLINSANDTECVKRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENVYGK 180

Query: 181 APTELADPGTEARTILEAASAQFAMGCQ 209
           APTEL DPGTEAR ILEAASA  A+GCQ
Sbjct: 181 APTELPDPGTEARMILEAASAHMAVGCQ 208

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BLAST of MS019614 vs. NCBI nr
Match: XP_022934112.1 (tankyrase-2 [Cucurbita moschata] >KAG7017388.1 BRCA1-associated RING domain protein 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 386.0 bits (990), Expect = 2.1e-103
Identity = 189/208 (90.87%), Postives = 201/208 (96.63%), Query Frame = 0


Query: 1   MAVPGRRNGMVDEDDYEEDNALFEEDGVMEFDSDTPPHLRPLATAAQLGDVNGLRSALDN 60
           MAVPGRRNG+VDEDDYEEDNALFEE+GV+EFDSDTPPHLR LATAAQLGDVNGLR+A+DN
Sbjct: 1   MAVPGRRNGVVDEDDYEEDNALFEENGVIEFDSDTPPHLRALATAAQLGDVNGLRNAIDN 60

Query: 61  LTMGSIDDAVEDGDTALHLTCLYGHLPCVQLLLERGANLEVEDEDGAIPLHDACAGGFLE 120
           LTMGSI+DAVEDGDT LHLTCLYGHLPCVQLL+ERGANLEV+DEDGAIPLHDACAGG++E
Sbjct: 61  LTMGSINDAVEDGDTVLHLTCLYGHLPCVQLLVERGANLEVQDEDGAIPLHDACAGGYVE 120

Query: 121 IVQLLINSANNTDCLKRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENTYGK 180
           IVQ LINSAN TD +KRM+ESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENTYGK
Sbjct: 121 IVQFLINSANGTDGVKRMVESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENTYGK 180

Query: 181 APTELADPGTEARTILEAASAQFAMGCQ 209
           APTELADPGTEARTILEAASA  AMGCQ
Sbjct: 181 APTELADPGTEARTILEAASAHMAMGCQ 208

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BLAST of MS019614 vs. NCBI nr
Match: XP_022983109.1 (tankyrase-2 [Cucurbita maxima])

HSP 1 Score: 386.0 bits (990), Expect = 2.1e-103
Identity = 189/208 (90.87%), Postives = 201/208 (96.63%), Query Frame = 0


Query: 1   MAVPGRRNGMVDEDDYEEDNALFEEDGVMEFDSDTPPHLRPLATAAQLGDVNGLRSALDN 60
           MAVPGRRNG+VDEDDYEEDNALFEE+GV+EFDSDTPPHLR LATAAQLGDVNGLR+A+DN
Sbjct: 1   MAVPGRRNGVVDEDDYEEDNALFEENGVIEFDSDTPPHLRALATAAQLGDVNGLRTAIDN 60

Query: 61  LTMGSIDDAVEDGDTALHLTCLYGHLPCVQLLLERGANLEVEDEDGAIPLHDACAGGFLE 120
           LTMGSI+DAVEDGDT LHLTCLYGHLPCVQLL+ERGANLEV+DEDGAIPLHDACAGG++E
Sbjct: 61  LTMGSINDAVEDGDTVLHLTCLYGHLPCVQLLVERGANLEVQDEDGAIPLHDACAGGYVE 120

Query: 121 IVQLLINSANNTDCLKRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENTYGK 180
           IVQ LINSAN TD +KRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENTYGK
Sbjct: 121 IVQFLINSANGTDGVKRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENTYGK 180

Query: 181 APTELADPGTEARTILEAASAQFAMGCQ 209
           APTELADPGTEARTILEAASA  AMGC+
Sbjct: 181 APTELADPGTEARTILEAASAHMAMGCE 208

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BLAST of MS019614 vs. NCBI nr
Match: XP_023526415.1 (tankyrase-1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 381.7 bits (979), Expect = 4.0e-102
Identity = 187/208 (89.90%), Postives = 200/208 (96.15%), Query Frame = 0


Query: 1   MAVPGRRNGMVDEDDYEEDNALFEEDGVMEFDSDTPPHLRPLATAAQLGDVNGLRSALDN 60
           MAVPGRRNG+VDEDDYEEDNALFEE+GV+EFDSDTPPHLR LATAAQLGDVNGLR+A+DN
Sbjct: 1   MAVPGRRNGVVDEDDYEEDNALFEENGVIEFDSDTPPHLRALATAAQLGDVNGLRNAIDN 60

Query: 61  LTMGSIDDAVEDGDTALHLTCLYGHLPCVQLLLERGANLEVEDEDGAIPLHDACAGGFLE 120
           LTMGSI+DAVEDGDT LHLTCLYGHLPCVQLL+ERGANLEV+DEDGAIPLHDA AGG++E
Sbjct: 61  LTMGSINDAVEDGDTVLHLTCLYGHLPCVQLLVERGANLEVQDEDGAIPLHDASAGGYVE 120

Query: 121 IVQLLINSANNTDCLKRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENTYGK 180
           IVQ LINSAN TD +KRM+ESVDAEGDTPLHHAARGEHAAV+RLLLASGASPTKENTYGK
Sbjct: 121 IVQFLINSANGTDGVKRMVESVDAEGDTPLHHAARGEHAAVIRLLLASGASPTKENTYGK 180

Query: 181 APTELADPGTEARTILEAASAQFAMGCQ 209
           APTELADPGTEARTILEAASA  AMGCQ
Sbjct: 181 APTELADPGTEARTILEAASAHMAMGCQ 208

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BLAST of MS019614 vs. ExPASy Swiss-Prot
Match: Q99728 (BRCA1-associated RING domain protein 1 OS=Homo sapiens OX=9606 GN=BARD1 PE=1 SV=2)

HSP 1 Score: 85.9 bits (211), Expect = 5.9e-16
Identity = 42/115 (36.52%), Postives = 67/115 (58.26%), Query Frame = 0


Query: 73  GDTALHLTCLYGHLPCVQLLLERGANLEVEDEDGAIPLHDACAGGFLEIVQLLINSANNT 132
           G+T LH+  + G +P V+ LL+ G++  V+D  G  PLH+AC  G L++V+LL+      
Sbjct: 428 GETLLHIASIKGDIPSVEYLLQNGSDPNVKDHAGWTPLHEACNHGHLKVVELLLQH---- 487

Query: 133 DCLKRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENTYGKAPTELAD 188
              K ++ +   + D+PLH AA+  H  +V+LLL+ GAS    N +G  P +  D
Sbjct: 488 ---KALVNTTGYQNDSPLHDAAKNGHVDIVKLLLSYGASRNAVNIFGLRPVDYTD 535

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BLAST of MS019614 vs. ExPASy Swiss-Prot
Match: Q9VBP3 (Poly [ADP-ribose] polymerase tankyrase OS=Drosophila melanogaster OX=7227 GN=Tnks PE=1 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 5.0e-15
Identity = 54/166 (32.53%), Postives = 78/166 (46.99%), Query Frame = 0


Query: 21  ALFEEDGVMEFDSDTPPHLRPLATAAQLGDVNGLRS-ALDNLTMGSIDDAVEDGDTALHL 80
           A    D V++   + P     L  AA+ GD++ +R   L+N    +  D      T LH 
Sbjct: 467 AQLASDSVLKLLKNPPDSETHLLEAAKAGDLDTVRRIVLNNPISVNCRDLDGRHSTPLHF 526

Query: 81  TCLYGHLPCVQLLLERGANLEVEDEDGAIPLHDACAGGFLEIVQLLINSANNTDCLKRML 140
              +  +P VQ LLE GA +   D+ G +PLH+AC+ G  E+ +LL+    N +      
Sbjct: 527 AAGFNRVPVVQFLLEHGAEVYAADKGGLVPLHNACSYGHYEVTELLVKHGANVNV----- 586

Query: 141 ESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENTYGKAPTEL 186
              D    TPLH AA      + +LLL  GA P K+N  G  P +L
Sbjct: 587 --SDLWKFTPLHEAAAKGKYDICKLLLKHGADPMKKNRDGATPADL 625

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BLAST of MS019614 vs. ExPASy Swiss-Prot
Match: Q9TU71 (Ankyrin repeat domain-containing protein 1 OS=Oryctolagus cuniculus OX=9986 GN=ANKRD1 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 8.6e-15
Identity = 49/137 (35.77%), Postives = 69/137 (50.36%), Query Frame = 0


Query: 75  TALHLTCLYGHLPCVQLLLERGANLEVEDEDGAIPLHDACAGGFLEIVQLLINS------ 134
           TALH  CL GHL  V+ L+E GA +E  D   +  +H AC GG LE+++LL+N       
Sbjct: 155 TALHRACLEGHLAIVEKLMEAGAQIEFRDMLESTAIHWACRGGNLEVLKLLLNKGAKISA 214

Query: 135 --------------ANNTDCLKRM------LESVDAEGDTPLHHAARGEHAAVVRLLLAS 186
                           + +C + +      L + D EGDTPLH A R     ++RLL+  
Sbjct: 215 RDKLLSTALHVAVRTGHYECAEHLIACEADLNAKDREGDTPLHDAVRLNRYKMIRLLIMY 274

[Close]

BLAST of MS019614 vs. ExPASy Swiss-Prot
Match: O70445 (BRCA1-associated RING domain protein 1 OS=Mus musculus OX=10090 GN=Bard1 PE=2 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 2.5e-14
Identity = 39/115 (33.91%), Postives = 65/115 (56.52%), Query Frame = 0


Query: 73  GDTALHLTCLYGHLPCVQLLLERGANLEVEDEDGAIPLHDACAGGFLEIVQLLINSANNT 132
           G+T LH+  + G +P V+ LL+ G +  V+D  G  PLH+AC+ G L++V+LL+      
Sbjct: 416 GETLLHIASIKGDIPSVEYLLQNGNDPNVKDHAGWTPLHEACSHGHLKVVELLLQH---- 475

Query: 133 DCLKRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENTYGKAPTELAD 188
                ++ +   + D+PLH A +  H  +V++LL+ GAS    N +G  P +  D
Sbjct: 476 ---NALVNTPGYQNDSPLHDAVKSGHIDIVKVLLSHGASRNAVNIFGVRPVDYTD 523

[Close]

BLAST of MS019614 vs. ExPASy Swiss-Prot
Match: Q9QZH2 (BRCA1-associated RING domain protein 1 OS=Rattus norvegicus OX=10116 GN=Bard1 PE=2 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 2.5e-14
Identity = 40/115 (34.78%), Postives = 65/115 (56.52%), Query Frame = 0


Query: 73  GDTALHLTCLYGHLPCVQLLLERGANLEVEDEDGAIPLHDACAGGFLEIVQLLINSANNT 132
           G+T LH+  + G +  V+ LL+ G +  V+D  G  PLH+AC+ G L+IV+LL+      
Sbjct: 418 GETLLHIASIKGDISSVEYLLQNGNDPNVKDHAGWTPLHEACSHGHLKIVELLLQH---- 477

Query: 133 DCLKRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENTYGKAPTELAD 188
                ++ +     D+PLH AA+  H  +V++LL+ GAS    N +G+ P +  D
Sbjct: 478 ---NALVNTTGYHNDSPLHDAAKNGHIDIVKVLLSHGASRNAVNIFGERPVDYTD 525

[Close]

BLAST of MS019614 vs. ExPASy TrEMBL
Match: A0A6J1CTX1 (tankyrase-2 OS=Momordica charantia OX=3673 GN=LOC111014248 PE=4 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 1.1e-113
Identity = 208/208 (100.00%), Postives = 208/208 (100.00%), Query Frame = 0


Query: 1   MAVPGRRNGMVDEDDYEEDNALFEEDGVMEFDSDTPPHLRPLATAAQLGDVNGLRSALDN 60
           MAVPGRRNGMVDEDDYEEDNALFEEDGVMEFDSDTPPHLRPLATAAQLGDVNGLRSALDN
Sbjct: 1   MAVPGRRNGMVDEDDYEEDNALFEEDGVMEFDSDTPPHLRPLATAAQLGDVNGLRSALDN 60

Query: 61  LTMGSIDDAVEDGDTALHLTCLYGHLPCVQLLLERGANLEVEDEDGAIPLHDACAGGFLE 120
           LTMGSIDDAVEDGDTALHLTCLYGHLPCVQLLLERGANLEVEDEDGAIPLHDACAGGFLE
Sbjct: 61  LTMGSIDDAVEDGDTALHLTCLYGHLPCVQLLLERGANLEVEDEDGAIPLHDACAGGFLE 120

Query: 121 IVQLLINSANNTDCLKRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENTYGK 180
           IVQLLINSANNTDCLKRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENTYGK
Sbjct: 121 IVQLLINSANNTDCLKRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENTYGK 180

Query: 181 APTELADPGTEARTILEAASAQFAMGCQ 209
           APTELADPGTEARTILEAASAQFAMGCQ
Sbjct: 181 APTELADPGTEARTILEAASAQFAMGCQ 208

[Close]

BLAST of MS019614 vs. ExPASy TrEMBL
Match: A0A6J1J6D4 (tankyrase-2 OS=Cucurbita maxima OX=3661 GN=LOC111481754 PE=4 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 1.0e-103
Identity = 189/208 (90.87%), Postives = 201/208 (96.63%), Query Frame = 0


Query: 1   MAVPGRRNGMVDEDDYEEDNALFEEDGVMEFDSDTPPHLRPLATAAQLGDVNGLRSALDN 60
           MAVPGRRNG+VDEDDYEEDNALFEE+GV+EFDSDTPPHLR LATAAQLGDVNGLR+A+DN
Sbjct: 1   MAVPGRRNGVVDEDDYEEDNALFEENGVIEFDSDTPPHLRALATAAQLGDVNGLRTAIDN 60

Query: 61  LTMGSIDDAVEDGDTALHLTCLYGHLPCVQLLLERGANLEVEDEDGAIPLHDACAGGFLE 120
           LTMGSI+DAVEDGDT LHLTCLYGHLPCVQLL+ERGANLEV+DEDGAIPLHDACAGG++E
Sbjct: 61  LTMGSINDAVEDGDTVLHLTCLYGHLPCVQLLVERGANLEVQDEDGAIPLHDACAGGYVE 120

Query: 121 IVQLLINSANNTDCLKRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENTYGK 180
           IVQ LINSAN TD +KRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENTYGK
Sbjct: 121 IVQFLINSANGTDGVKRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENTYGK 180

Query: 181 APTELADPGTEARTILEAASAQFAMGCQ 209
           APTELADPGTEARTILEAASA  AMGC+
Sbjct: 181 APTELADPGTEARTILEAASAHMAMGCE 208

[Close]

BLAST of MS019614 vs. ExPASy TrEMBL
Match: A0A6J1F1S2 (tankyrase-2 OS=Cucurbita moschata OX=3662 GN=LOC111441383 PE=4 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 1.0e-103
Identity = 189/208 (90.87%), Postives = 201/208 (96.63%), Query Frame = 0


Query: 1   MAVPGRRNGMVDEDDYEEDNALFEEDGVMEFDSDTPPHLRPLATAAQLGDVNGLRSALDN 60
           MAVPGRRNG+VDEDDYEEDNALFEE+GV+EFDSDTPPHLR LATAAQLGDVNGLR+A+DN
Sbjct: 1   MAVPGRRNGVVDEDDYEEDNALFEENGVIEFDSDTPPHLRALATAAQLGDVNGLRNAIDN 60

Query: 61  LTMGSIDDAVEDGDTALHLTCLYGHLPCVQLLLERGANLEVEDEDGAIPLHDACAGGFLE 120
           LTMGSI+DAVEDGDT LHLTCLYGHLPCVQLL+ERGANLEV+DEDGAIPLHDACAGG++E
Sbjct: 61  LTMGSINDAVEDGDTVLHLTCLYGHLPCVQLLVERGANLEVQDEDGAIPLHDACAGGYVE 120

Query: 121 IVQLLINSANNTDCLKRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENTYGK 180
           IVQ LINSAN TD +KRM+ESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENTYGK
Sbjct: 121 IVQFLINSANGTDGVKRMVESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENTYGK 180

Query: 181 APTELADPGTEARTILEAASAQFAMGCQ 209
           APTELADPGTEARTILEAASA  AMGCQ
Sbjct: 181 APTELADPGTEARTILEAASAHMAMGCQ 208

[Close]

BLAST of MS019614 vs. ExPASy TrEMBL
Match: A0A5D3DNG9 (Tankyrase-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002930 PE=4 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 5.6e-102
Identity = 187/209 (89.47%), Postives = 202/209 (96.65%), Query Frame = 0


Query: 1   MAVPGRRNGMVDEDDYEEDNALFEEDGVMEFDSDTPPHLRPLATAAQLGDVNGLRSALDN 60
           MAVPGRRNG+VDEDDYEEDNALFEEDGV+EFDSDTPPHLRPLA+A+QLGD++GLR+ALDN
Sbjct: 1   MAVPGRRNGVVDEDDYEEDNALFEEDGVIEFDSDTPPHLRPLASASQLGDIDGLRTALDN 60

Query: 61  LTMGSIDDAVEDGDTALHLTCLYGHLPCVQLLLERGANLEVEDEDGAIPLHDACAGGFLE 120
           LT+GSI+DAVEDGDTALHLTCLYGHLPCV+LLLERGA LEV+DEDGAIPLHDACAGGF+E
Sbjct: 61  LTVGSINDAVEDGDTALHLTCLYGHLPCVRLLLERGAALEVQDEDGAIPLHDACAGGFVE 120

Query: 121 IVQLLINSANNTDCLKRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENTYGK 180
           IVQLLINSAN+T+C+KRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTK NTYGK
Sbjct: 121 IVQLLINSANDTECVKRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKRNTYGK 180

Query: 181 APTELADPGTEARTILE-AASAQFAMGCQ 209
           APTEL DPGTEAR ILE AASA  AMGCQ
Sbjct: 181 APTELPDPGTEARAILEAAASAHMAMGCQ 209

[Close]

BLAST of MS019614 vs. ExPASy TrEMBL
Match: A0A1S3B6E0 (tankyrase-2 OS=Cucumis melo OX=3656 GN=LOC103486520 PE=4 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 5.6e-102
Identity = 187/209 (89.47%), Postives = 202/209 (96.65%), Query Frame = 0


Query: 1   MAVPGRRNGMVDEDDYEEDNALFEEDGVMEFDSDTPPHLRPLATAAQLGDVNGLRSALDN 60
           MAVPGRRNG+VDEDDYEEDNALFEEDGV+EFDSDTPPHLRPLA+A+QLGD++GLR+ALDN
Sbjct: 1   MAVPGRRNGVVDEDDYEEDNALFEEDGVIEFDSDTPPHLRPLASASQLGDIDGLRTALDN 60

Query: 61  LTMGSIDDAVEDGDTALHLTCLYGHLPCVQLLLERGANLEVEDEDGAIPLHDACAGGFLE 120
           LT+GSI+DAVEDGDTALHLTCLYGHLPCV+LLLERGA LEV+DEDGAIPLHDACAGGF+E
Sbjct: 61  LTVGSINDAVEDGDTALHLTCLYGHLPCVRLLLERGAALEVQDEDGAIPLHDACAGGFVE 120

Query: 121 IVQLLINSANNTDCLKRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENTYGK 180
           IVQLLINSAN+T+C+KRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTK NTYGK
Sbjct: 121 IVQLLINSANDTECVKRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKRNTYGK 180

Query: 181 APTELADPGTEARTILE-AASAQFAMGCQ 209
           APTEL DPGTEAR ILE AASA  AMGCQ
Sbjct: 181 APTELPDPGTEARAILEAAASAHMAMGCQ 209

[Close]

BLAST of MS019614 vs. TAIR 10
Match: AT3G09890.1 (Ankyrin repeat family protein )

HSP 1 Score: 263.8 bits (673), Expect = 1.1e-70
Identity = 131/200 (65.50%), Postives = 161/200 (80.50%), Query Frame = 0


Query: 1   MAVPGRRNGMVDEDDYEEDNALFEEDGV-MEFDSDTPPHLRPLATAAQLGDVNGLRSALD 60
           MAVP   NG+ +E+D + +NA+FEE+GV  + DS+ P HLR LA AAQ GDV  LR+A+D
Sbjct: 1   MAVPRGGNGLNEEEDVDGENAIFEENGVDYDTDSELPSHLRDLAAAAQAGDVAALRTAID 60

Query: 61  NLTMGSIDDAVEDGDTALHLTCLYGHLPCVQLLLERGANLEVEDEDGAIPLHDACAGGFL 120
           NL  G +D+ +ED D+ALHL CLYGHLPCVQLLLERGA++EV+DED AIPLHDACAGG+L
Sbjct: 61  NLN-GRVDEPLEDNDSALHLACLYGHLPCVQLLLERGADMEVKDEDEAIPLHDACAGGYL 120

Query: 121 EIVQLLINSANNTDCLKRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENTYG 180
           EIVQLL + A++ +C+KRM+E+ D EGDTPLHHAARGEH  VVR LL SGASPT +N+YG
Sbjct: 121 EIVQLLFSRASSPECVKRMIETADIEGDTPLHHAARGEHVDVVRFLLGSGASPTTQNSYG 180

Query: 181 KAPTELADPGTEARTILEAA 200
           K P ELAD  T+A+ ILE A
Sbjct: 181 KTPGELADLNTDAKRILEEA 199

[Close]

BLAST of MS019614 vs. TAIR 10
Match: AT3G09890.2 (Ankyrin repeat family protein )

HSP 1 Score: 194.9 bits (494), Expect = 6.4e-50
Identity = 95/149 (63.76%), Postives = 121/149 (81.21%), Query Frame = 0


Query: 1   MAVPGRRNGMVDEDDYEEDNALFEEDGV-MEFDSDTPPHLRPLATAAQLGDVNGLRSALD 60
           MAVP   NG+ +E+D + +NA+FEE+GV  + DS+ P HLR LA AAQ GDV  LR+A+D
Sbjct: 1   MAVPRGGNGLNEEEDVDGENAIFEENGVDYDTDSELPSHLRDLAAAAQAGDVAALRTAID 60

Query: 61  NLTMGSIDDAVEDGDTALHLTCLYGHLPCVQLLLERGANLEVEDEDGAIPLHDACAGGFL 120
           NL  G +D+ +ED D+ALHL CLYGHLPCVQLLLERGA++EV+DED AIPLHDACAGG+L
Sbjct: 61  NLN-GRVDEPLEDNDSALHLACLYGHLPCVQLLLERGADMEVKDEDEAIPLHDACAGGYL 120

Query: 121 EIVQLLINSANNTDCLKRMLESVDAEGDT 149
           EIVQLL + A++ +C+KRM+E+ D EGDT
Sbjct: 121 EIVQLLFSRASSPECVKRMIETADIEGDT 148

[Close]

BLAST of MS019614 vs. TAIR 10
Match: AT5G40160.1 (Ankyrin repeat family protein )

HSP 1 Score: 73.9 bits (180), Expect = 1.7e-13
Identity = 58/184 (31.52%), Postives = 89/184 (48.37%), Query Frame = 0


Query: 14  DDYEEDNA-----LFEEDGVMEFDSDTPPHLRPLATAA----QLGDVNGLRSALDNLTMG 73
           D+YEE+ A     L E +  +    +  P+L+ ++T +    Q   ++     +DNL   
Sbjct: 115 DNYEEELAKEVEQLLEPEERVILQQNEKPNLKMISTKSWKPLQTLALSMQIQLMDNLIEN 174

Query: 74  --SIDDAVEDGDTALHLTCLYGHLPCVQLLLERGANLEVEDEDGAIPLHDACAGGFLEIV 133
              IDD  +D  TALH   +      +  LL +GAN  ++D DGA P+H A   G L+ V
Sbjct: 175 GLDIDDVDKDNQTALHKAIIGKKEAVISHLLRKGANPHLQDRDGAAPIHYAVQVGALQTV 234

Query: 134 QLLINSANNTDCLKRMLESVDAEGDTPLHHAARGEHAAVVRLLLASGASPTKENTYGKAP 187
           +LL     N D     +   D EG TPLH A +  +  + ++LL +GA  T+    GK  
Sbjct: 235 KLLFK--YNVD-----VNVADNEGWTPLHIAVQSRNRDITKILLTNGADKTRRTKDGKLA 291

[Close]

BLAST of MS019614 vs. TAIR 10
Match: AT4G35450.3 (ankyrin repeat-containing protein 2 )

HSP 1 Score: 70.1 bits (170), Expect = 2.4e-12
Identity = 47/121 (38.84%), Postives = 67/121 (55.37%), Query Frame = 0


Query: 46  AQLGDVNGLRSALDNLTMGSIDDAVEDGDTALHLTCLYGHLPCVQLLLERGANLEVEDED 105
           A LGDV GL++AL   + G+ D+   +G TALH  C YG L C Q+L++ GA++   D++
Sbjct: 226 ASLGDVEGLKAAL--ASGGNKDEEDSEGRTALHFACGYGELKCAQVLIDAGASVNAVDKN 285

Query: 106 GAIPLHDACAGGFLEIVQLLI-NSANNTDCLKRMLESVDAEGDTPLHHAARGEHAAVVRL 165
              PLH A   G  E V LL+ N A  T      L+++D +  TP+  A       VV+L
Sbjct: 286 KNTPLHYAAGYGRKECVSLLLENGAAVT------LQNLDEK--TPIDVAKLNSQLEVVKL 336

[Close]

BLAST of MS019614 vs. TAIR 10
Match: AT4G35450.4 (ankyrin repeat-containing protein 2 )

HSP 1 Score: 70.1 bits (170), Expect = 2.4e-12
Identity = 47/121 (38.84%), Postives = 67/121 (55.37%), Query Frame = 0


Query: 46  AQLGDVNGLRSALDNLTMGSIDDAVEDGDTALHLTCLYGHLPCVQLLLERGANLEVEDED 105
           A LGDV GL++AL   + G+ D+   +G TALH  C YG L C Q+L++ GA++   D++
Sbjct: 188 ASLGDVEGLKAAL--ASGGNKDEEDSEGRTALHFACGYGELKCAQVLIDAGASVNAVDKN 247

Query: 106 GAIPLHDACAGGFLEIVQLLI-NSANNTDCLKRMLESVDAEGDTPLHHAARGEHAAVVRL 165
              PLH A   G  E V LL+ N A  T      L+++D +  TP+  A       VV+L
Sbjct: 248 KNTPLHYAAGYGRKECVSLLLENGAAVT------LQNLDEK--TPIDVAKLNSQLEVVKL 298

The following BLAST results are available for this feature:

BLAST of MS019614 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022144606.1	2.3e-113	100.00	tankyrase-2 [Momordica charantia]	[more]
XP_038905294.1	2.1e-103	91.35	poly [ADP-ribose] polymerase tankyrase [Benincasa hispida]	[more]
XP_022934112.1	2.1e-103	90.87	tankyrase-2 [Cucurbita moschata] >KAG7017388.1 BRCA1-associated RING domain prot...	[more]
XP_022983109.1	2.1e-103	90.87	tankyrase-2 [Cucurbita maxima]	[more]
XP_023526415.1	4.0e-102	89.90	tankyrase-1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS019614 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q99728	5.9e-16	36.52	BRCA1-associated RING domain protein 1 OS=Homo sapiens OX=9606 GN=BARD1 PE=1 SV=...	[more]
Q9VBP3	5.0e-15	32.53	Poly [ADP-ribose] polymerase tankyrase OS=Drosophila melanogaster OX=7227 GN=Tnk...	[more]
Q9TU71	8.6e-15	35.77	Ankyrin repeat domain-containing protein 1 OS=Oryctolagus cuniculus OX=9986 GN=A...	[more]
O70445	2.5e-14	33.91	BRCA1-associated RING domain protein 1 OS=Mus musculus OX=10090 GN=Bard1 PE=2 SV...	[more]
Q9QZH2	2.5e-14	34.78	BRCA1-associated RING domain protein 1 OS=Rattus norvegicus OX=10116 GN=Bard1 PE...	[more]

BLAST of MS019614 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CTX1	1.1e-113	100.00	tankyrase-2 OS=Momordica charantia OX=3673 GN=LOC111014248 PE=4 SV=1	[more]
A0A6J1J6D4	1.0e-103	90.87	tankyrase-2 OS=Cucurbita maxima OX=3661 GN=LOC111481754 PE=4 SV=1	[more]
A0A6J1F1S2	1.0e-103	90.87	tankyrase-2 OS=Cucurbita moschata OX=3662 GN=LOC111441383 PE=4 SV=1	[more]
A0A5D3DNG9	5.6e-102	89.47	Tankyrase-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002930 P...	[more]
A0A1S3B6E0	5.6e-102	89.47	tankyrase-2 OS=Cucumis melo OX=3656 GN=LOC103486520 PE=4 SV=1	[more]

BLAST of MS019614 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G09890.1	1.1e-70	65.50	Ankyrin repeat family protein	[more]
AT3G09890.2	6.4e-50	63.76	Ankyrin repeat family protein	[more]
AT5G40160.1	1.7e-13	31.52	Ankyrin repeat family protein	[more]
AT4G35450.3	2.4e-12	38.84	ankyrin repeat-containing protein 2	[more]
AT4G35450.4	2.4e-12	38.84	ankyrin repeat-containing protein 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002110	Ankyrin repeat	SMART	SM00248	ANK_2a	coord: 145..174 e-value: 4.4E-5 score: 32.9 coord: 105..134 e-value: 0.014 score: 24.6 coord: 72..101 e-value: 3.3E-6 score: 36.6
IPR002110	Ankyrin repeat	PFAM	PF00023	Ank	coord: 72..103 e-value: 3.4E-7 score: 30.4
IPR002110	Ankyrin repeat	PROSITE	PS50088	ANK_REPEAT	coord: 72..104 score: 14.05163
IPR002110	Ankyrin repeat	PROSITE	PS50088	ANK_REPEAT	coord: 105..137 score: 9.61777
IPR002110	Ankyrin repeat	PROSITE	PS50088	ANK_REPEAT	coord: 145..177 score: 12.84968
IPR036770	Ankyrin repeat-containing domain superfamily	GENE3D	1.25.40.20		coord: 36..205 e-value: 3.3E-36 score: 126.3
IPR036770	Ankyrin repeat-containing domain superfamily	SUPERFAMILY	48403	Ankyrin repeat	coord: 32..186
IPR020683	Ankyrin repeat-containing domain	PFAM	PF12796	Ank_2	coord: 105..175 e-value: 5.2E-11 score: 43.0
None	No IPR available	PANTHER	PTHR24136:SF15	SOWAH (DROSOPHILA) HOMOLOG	coord: 1..201
None	No IPR available	PANTHER	PTHR24136	SOWAH (DROSOPHILA) HOMOLOG	coord: 1..201
None	No IPR available	PROSITE	PS50297	ANK_REP_REGION	coord: 145..171 score: 11.665081
None	No IPR available	PROSITE	PS50297	ANK_REP_REGION	coord: 105..133 score: 9.011944
None	No IPR available	PROSITE	PS50297	ANK_REP_REGION	coord: 72..104 score: 12.673273

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS019614.1	MS019614.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005515	protein binding