MS019549 (gene) Bitter gourd (TR) v1

Overview
NameMS019549
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionMonodehydroascorbate reductase
Locationscaffold729: 366733 .. 369680 (-)
RNA-Seq ExpressionMS019549
SyntenyMS019549
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTGCTCCTTATGAACGCCCTGCCCTTAGTAAGGGATATCTCTTCCCTGAGTGTAAGTCTTTTATTTATGTAAAAGTTTTCAACGAGAAAGTTATTGACTTGATCTTATCTTATCTTTTTCTTTTTTTTTTGGGATATCACATTGCAACTTGACAAGTTTGTTTCTTCAGCTCCTGCCAGACTCCCAGGATTCCACGTCTGTGTTGGAAGTGGAGGAGAGAAACTTTTACCAGAGTGGTACAAAGAAAAAGGTAATTGTTGCTTTTTTACCTTCTGCTAACCCATGATGCATGAGTAAGCCATTTCTATAAATAACATTTCTTGCTTATTATATTTTTAACTCTTTAGTTAGGTTTCCTTGTTGACTATTCTTGGTAATTATCTTCTGATTGATAATCTACTTATATTTCAGGGATTGAACTGATCCTTAGCACGGAAATAGTTAAAGCAGATCTTGCTGCCAAGGCTCTCACTAGTGCCTCTGGTCAAACCTTCAAGTATCAAATTTTGATCATTGCAACGGGTTCCACTGTAAACTCACTGACTTACACATAACACTTATGCTGATCTGTCCTCATTTCAGATTGAACTTATGTCAGTTTTGTCGTTTTGCAGGTCATAAGATTGTCAGATTTTGGTGTACAAGGAGCTGACGCCAAAAACATTTTCTATTTGAGAGAGGTTGATGATGCCGACAAGCTTGTAGAAGCAATTAAAGCAAAGAAGAGTGGGAAGGCTGTGATTGTGGGAGGAGGATACATTGGTCTTGAACTTGGTGCAGCATTACAGATGAACAATCTTGATGTTACCATGGTCTACCCTGAGCCATGGTGCAGTAAGTATTAAAATAAAGAGGGTTCTTGAATTTCCATTTTATGCCCTCTGTAGACGTCTTTTTTTTTCTTTTGCCAAAGCCTTAGTTTTCTCTTGTGGTTTGGTTCCTGCAGTGCCCAGGCTTTTCACATCTGGTATAGCTGCCTTCTATGAAGGTTACTACAAAAACAAAGGAATCAATATAATTAAAGGAACAGTAGCAGTTGGTTTTACCTCAGATTCAAATGGAGAGGTTAGTAGCTTGTTTCAATGTTACCTTATGCCTCCTGCCTTGATGATGCTTTATAGAAAAACAATGTCATGAAGCTGATCTTTCAGGTGAAGCAAGTCAAACTCAAAGATGGCAGGGTCCTTGATGCCGACATTGTTGTTGTGGGTGTTGGAGGCAGACCCTTGATATCTTTATTTAAAGGACAGGTCGAAGAAGAGAAGGGAGGCATTAAGGTTAGTAAGGCTAATTTAGTTTTTTCACGATAGTTTTTCCCAACATCAACATTTTTACAATACTGGTAAAACTATATTTTTGTCTTAGGTAAAAATAAACTTTAATTTTGGTCCCTGGTTCCTAAAATTTACAAGTAATGTTATGTTAATACCTATATGGACAATGTTTCCCAGTTGTTCTTTAAACTAATAATGGCCTATCATTAAAATGAATTAAGTGAGACAATTGTAAAGTTCAAATAATGCCAAATTTAAAATTTGACAGACCATACAAAATTTAAAAACATTAGGGACCAAAATGAAAACGACAGTTAATATCTAGCCAAATTTCTTCTAGCATTGCAAAATTCATTTGCCTTTCCACCTGTTTCATTTTCATGTTCTTACAAAGAATGAAGATTCAATAAATTAGCACGAAATGAAAGAGACAATCGCTTGAAGAAAGAAACTTTTATTAGGAAAATATGAATATGGACACTCTTCGAGAGAAGACTCTCTCACACTTTCTAACTTTCCTCACTTTCTTCACTCACTTCACTTGATTTACCTCTGGGGACATCTCTCCTATTTATAGAAGGGTCACTGTAGTCTAAGTAAATAGATTGTTCTAGATCTCTCTACAAAGTATCCTAGATACTTCTTAATGTATCCTTATCCAAAAGAATTCTCTAGAATCCTTTGGAGCCTAAGTTAAAATATTTCTAAAGTTTTTTCTTTAGATCCTTATTTACAATCTTCCAGCAATCTAGAAAGATTAATACTTTAGATATTTATTTACAATCTTCCAGAACAATCTAGAAAGACTAATCCATTTAAGAGAGACACGTAGAAACTCCAAGAAACTTCTAGATAATTAGTGTTGTATTTTAACATCTATATCTTCTTCTTCTAATTCCTCTAGCATGTGTAGATTTGACAGTCAAGTTTTATGAACTCATACATTAGCTTATTGATGTCAGACTGATGGATTCTTCAAAACAAGCATTCCTGGCGTGTATGCTGTGGGAGACGTGGCTACTTACCCCTTGAAGCTATACAACGAGTTGAGAAGAGTTGAACACGTTGACCACGCTCGCAAATCTGCCGAGCAGGCTGTGAAGGTGTTTAACTTCCCTTGACAATTTTTATTTAACAATTGAGAAGATGAGAAGATCCCAATATATCGTTTAAGGAAAATACATAGATCCTTACTCGTGCTGACCATCATTTGAATATTGAAAAATAGAGAATATTTTTAAAAAATAATGAGTGGTCAAATATTCAATAATTATCAGCCGGCAAGGATCTAAAGTATTTTCCATCATTTAATTCCCCACGATATATATCTGACACTTTCTTTTTCTTGATAGGCCATCAAGGCACAGGAAGAGGGCAAATCAACTGATGTGTATGACTACCTCCCATACTTCTATTCTCGCGCCTTCAATCTGTCATGGCAATTCTACGGCGATAACGTCGGTGAAACCATCCTATTTGGAGACAACAACCCGGAATCATCTCAACCCAAGTTCGGAACCTACTGGGTCAAAGATGGAAAAGTAGTTGGAGTTTTCTTGGAGGGTGGAAGCGCGGATGAATACAAGGCCATCGCCAAGCTTGCACGAGTCCAACCATCAGTTCAGAGCTTGGATCAGCTCGCCAAAGAAGGTCTCTCCTTCGCCTCCAAGGTC

mRNA sequence

GTTGCTCCTTATGAACGCCCTGCCCTTAGTAAGGGATATCTCTTCCCTGAGTCTCCTGCCAGACTCCCAGGATTCCACGTCTGTGTTGGAAGTGGAGGAGAGAAACTTTTACCAGAGTGGTACAAAGAAAAAGGGATTGAACTGATCCTTAGCACGGAAATAGTTAAAGCAGATCTTGCTGCCAAGGCTCTCACTAGTGCCTCTGGTCAAACCTTCAAGTATCAAATTTTGATCATTGCAACGGGTTCCACTGTCATAAGATTGTCAGATTTTGGTGTACAAGGAGCTGACGCCAAAAACATTTTCTATTTGAGAGAGGTTGATGATGCCGACAAGCTTGTAGAAGCAATTAAAGCAAAGAAGAGTGGGAAGGCTGTGATTGTGGGAGGAGGATACATTGGTCTTGAACTTGGTGCAGCATTACAGATGAACAATCTTGATGTTACCATGGTCTACCCTGAGCCATGGTGCATGCCCAGGCTTTTCACATCTGGTATAGCTGCCTTCTATGAAGGTTACTACAAAAACAAAGGAATCAATATAATTAAAGGAACAGTAGCAGTTGGTTTTACCTCAGATTCAAATGGAGAGGTGAAGCAAGTCAAACTCAAAGATGGCAGGGTCCTTGATGCCGACATTGTTGTTGTGGGTGTTGGAGGCAGACCCTTGATATCTTTATTTAAAGGACAGGTCGAAGAAGAGAAGGGAGGCATTAAGACTGATGGATTCTTCAAAACAAGCATTCCTGGCGTGTATGCTGTGGGAGACGTGGCTACTTACCCCTTGAAGCTATACAACGAGTTGAGAAGAGTTGAACACGTTGACCACGCTCGCAAATCTGCCGAGCAGGCTGTGAAGGCCATCAAGGCACAGGAAGAGGGCAAATCAACTGATGTGTATGACTACCTCCCATACTTCTATTCTCGCGCCTTCAATCTGTCATGGCAATTCTACGGCGATAACGTCGGTGAAACCATCCTATTTGGAGACAACAACCCGGAATCATCTCAACCCAAGTTCGGAACCTACTGGGTCAAAGATGGAAAAGTAGTTGGAGTTTTCTTGGAGGGTGGAAGCGCGGATGAATACAAGGCCATCGCCAAGCTTGCACGAGTCCAACCATCAGTTCAGAGCTTGGATCAGCTCGCCAAAGAAGGTCTCTCCTTCGCCTCCAAGGTC

Coding sequence (CDS)

GTTGCTCCTTATGAACGCCCTGCCCTTAGTAAGGGATATCTCTTCCCTGAGTCTCCTGCCAGACTCCCAGGATTCCACGTCTGTGTTGGAAGTGGAGGAGAGAAACTTTTACCAGAGTGGTACAAAGAAAAAGGGATTGAACTGATCCTTAGCACGGAAATAGTTAAAGCAGATCTTGCTGCCAAGGCTCTCACTAGTGCCTCTGGTCAAACCTTCAAGTATCAAATTTTGATCATTGCAACGGGTTCCACTGTCATAAGATTGTCAGATTTTGGTGTACAAGGAGCTGACGCCAAAAACATTTTCTATTTGAGAGAGGTTGATGATGCCGACAAGCTTGTAGAAGCAATTAAAGCAAAGAAGAGTGGGAAGGCTGTGATTGTGGGAGGAGGATACATTGGTCTTGAACTTGGTGCAGCATTACAGATGAACAATCTTGATGTTACCATGGTCTACCCTGAGCCATGGTGCATGCCCAGGCTTTTCACATCTGGTATAGCTGCCTTCTATGAAGGTTACTACAAAAACAAAGGAATCAATATAATTAAAGGAACAGTAGCAGTTGGTTTTACCTCAGATTCAAATGGAGAGGTGAAGCAAGTCAAACTCAAAGATGGCAGGGTCCTTGATGCCGACATTGTTGTTGTGGGTGTTGGAGGCAGACCCTTGATATCTTTATTTAAAGGACAGGTCGAAGAAGAGAAGGGAGGCATTAAGACTGATGGATTCTTCAAAACAAGCATTCCTGGCGTGTATGCTGTGGGAGACGTGGCTACTTACCCCTTGAAGCTATACAACGAGTTGAGAAGAGTTGAACACGTTGACCACGCTCGCAAATCTGCCGAGCAGGCTGTGAAGGCCATCAAGGCACAGGAAGAGGGCAAATCAACTGATGTGTATGACTACCTCCCATACTTCTATTCTCGCGCCTTCAATCTGTCATGGCAATTCTACGGCGATAACGTCGGTGAAACCATCCTATTTGGAGACAACAACCCGGAATCATCTCAACCCAAGTTCGGAACCTACTGGGTCAAAGATGGAAAAGTAGTTGGAGTTTTCTTGGAGGGTGGAAGCGCGGATGAATACAAGGCCATCGCCAAGCTTGCACGAGTCCAACCATCAGTTCAGAGCTTGGATCAGCTCGCCAAAGAAGGTCTCTCCTTCGCCTCCAAGGTC

Protein sequence

VAPYERPALSKGYLFPESPARLPGFHVCVGSGGEKLLPEWYKEKGIELILSTEIVKADLAAKALTSASGQTFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAKKSGKAVIVGGGYIGLELGAALQMNNLDVTMVYPEPWCMPRLFTSGIAAFYEGYYKNKGINIIKGTVAVGFTSDSNGEVKQVKLKDGRVLDADIVVVGVGGRPLISLFKGQVEEEKGGIKTDGFFKTSIPGVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAQEEGKSTDVYDYLPYFYSRAFNLSWQFYGDNVGETILFGDNNPESSQPKFGTYWVKDGKVVGVFLEGGSADEYKAIAKLARVQPSVQSLDQLAKEGLSFASKV
Homology
BLAST of MS019549 vs. NCBI nr
Match: XP_022144639.1 (monodehydroascorbate reductase, seedling isozyme-like [Momordica charantia])

HSP 1 Score: 784.6 bits (2025), Expect = 3.9e-223
Identity = 393/393 (100.00%), Postives = 393/393 (100.00%), Query Frame = 0

Query: 1   VAPYERPALSKGYLFPESPARLPGFHVCVGSGGEKLLPEWYKEKGIELILSTEIVKADLA 60
           VAPYERPALSKGYLFPESPARLPGFHVCVGSGGEKLLPEWYKEKGIELILSTEIVKADLA
Sbjct: 42  VAPYERPALSKGYLFPESPARLPGFHVCVGSGGEKLLPEWYKEKGIELILSTEIVKADLA 101

Query: 61  AKALTSASGQTFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK 120
           AKALTSASGQTFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK
Sbjct: 102 AKALTSASGQTFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK 161

Query: 121 KSGKAVIVGGGYIGLELGAALQMNNLDVTMVYPEPWCMPRLFTSGIAAFYEGYYKNKGIN 180
           KSGKAVIVGGGYIGLELGAALQMNNLDVTMVYPEPWCMPRLFTSGIAAFYEGYYKNKGIN
Sbjct: 162 KSGKAVIVGGGYIGLELGAALQMNNLDVTMVYPEPWCMPRLFTSGIAAFYEGYYKNKGIN 221

Query: 181 IIKGTVAVGFTSDSNGEVKQVKLKDGRVLDADIVVVGVGGRPLISLFKGQVEEEKGGIKT 240
           IIKGTVAVGFTSDSNGEVKQVKLKDGRVLDADIVVVGVGGRPLISLFKGQVEEEKGGIKT
Sbjct: 222 IIKGTVAVGFTSDSNGEVKQVKLKDGRVLDADIVVVGVGGRPLISLFKGQVEEEKGGIKT 281

Query: 241 DGFFKTSIPGVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAQEEGKSTDVY 300
           DGFFKTSIPGVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAQEEGKSTDVY
Sbjct: 282 DGFFKTSIPGVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAQEEGKSTDVY 341

Query: 301 DYLPYFYSRAFNLSWQFYGDNVGETILFGDNNPESSQPKFGTYWVKDGKVVGVFLEGGSA 360
           DYLPYFYSRAFNLSWQFYGDNVGETILFGDNNPESSQPKFGTYWVKDGKVVGVFLEGGSA
Sbjct: 342 DYLPYFYSRAFNLSWQFYGDNVGETILFGDNNPESSQPKFGTYWVKDGKVVGVFLEGGSA 401

Query: 361 DEYKAIAKLARVQPSVQSLDQLAKEGLSFASKV 394
           DEYKAIAKLARVQPSVQSLDQLAKEGLSFASKV
Sbjct: 402 DEYKAIAKLARVQPSVQSLDQLAKEGLSFASKV 434

BLAST of MS019549 vs. NCBI nr
Match: XP_022934571.1 (monodehydroascorbate reductase, seedling isozyme-like [Cucurbita moschata] >KAG6580684.1 Monodehydroascorbate reductase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017439.1 Monodehydroascorbate reductase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 745.0 bits (1922), Expect = 3.4e-211
Identity = 368/393 (93.64%), Postives = 385/393 (97.96%), Query Frame = 0

Query: 1   VAPYERPALSKGYLFPESPARLPGFHVCVGSGGEKLLPEWYKEKGIELILSTEIVKADLA 60
           VAPYERPALSKGYLFPESPARLPGFHVCVGSGGE+LLPEWYKE GIELILSTEIVKADLA
Sbjct: 42  VAPYERPALSKGYLFPESPARLPGFHVCVGSGGERLLPEWYKENGIELILSTEIVKADLA 101

Query: 61  AKALTSASGQTFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK 120
           AKAL+++SG TFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK
Sbjct: 102 AKALSTSSGDTFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK 161

Query: 121 KSGKAVIVGGGYIGLELGAALQMNNLDVTMVYPEPWCMPRLFTSGIAAFYEGYYKNKGIN 180
           K+GKAVIVGGGYIGLELGAAL++NNLDVTMVYPEPWCMPRLFT+GIAAFYEG+YKNKGIN
Sbjct: 162 KNGKAVIVGGGYIGLELGAALKINNLDVTMVYPEPWCMPRLFTAGIAAFYEGFYKNKGIN 221

Query: 181 IIKGTVAVGFTSDSNGEVKQVKLKDGRVLDADIVVVGVGGRPLISLFKGQVEEEKGGIKT 240
           IIKGTVAVGFTSDSNGEVKQV+LKDGR LDADIVVVGVGGRPLISLFKGQVEEEKGGIKT
Sbjct: 222 IIKGTVAVGFTSDSNGEVKQVELKDGRSLDADIVVVGVGGRPLISLFKGQVEEEKGGIKT 281

Query: 241 DGFFKTSIPGVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAQEEGKSTDVY 300
           DGFFKTSIP VYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKA+EEG S + Y
Sbjct: 282 DGFFKTSIPDVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAKEEGNSIEEY 341

Query: 301 DYLPYFYSRAFNLSWQFYGDNVGETILFGDNNPESSQPKFGTYWVKDGKVVGVFLEGGSA 360
           DYLPYFYSR+FNLSWQFYGDNVGE ILFGDNNPES++PKFGTYWVKDGKVVGVFLEGG+A
Sbjct: 342 DYLPYFYSRSFNLSWQFYGDNVGEAILFGDNNPESAEPKFGTYWVKDGKVVGVFLEGGTA 401

Query: 361 DEYKAIAKLARVQPSVQSLDQLAKEGLSFASKV 394
           DEYKAIAKLARVQPSV+SLDQLAKEGLSFASKV
Sbjct: 402 DEYKAIAKLARVQPSVESLDQLAKEGLSFASKV 434

BLAST of MS019549 vs. NCBI nr
Match: XP_022982953.1 (monodehydroascorbate reductase, seedling isozyme-like [Cucurbita maxima])

HSP 1 Score: 743.4 bits (1918), Expect = 9.9e-211
Identity = 367/393 (93.38%), Postives = 384/393 (97.71%), Query Frame = 0

Query: 1   VAPYERPALSKGYLFPESPARLPGFHVCVGSGGEKLLPEWYKEKGIELILSTEIVKADLA 60
           VAPYERPALSKGYLFPESPARLPGFHVCVGSGGE+LLPEWYKE GIELILSTEIVKADLA
Sbjct: 42  VAPYERPALSKGYLFPESPARLPGFHVCVGSGGERLLPEWYKENGIELILSTEIVKADLA 101

Query: 61  AKALTSASGQTFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK 120
           AKAL+++SG TFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK
Sbjct: 102 AKALSTSSGDTFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK 161

Query: 121 KSGKAVIVGGGYIGLELGAALQMNNLDVTMVYPEPWCMPRLFTSGIAAFYEGYYKNKGIN 180
           K+GKAVIVGGGYIGLELGAAL++NNLDVTMVYPEPWCMPRLFT+GIAAFYEG+YKNKGIN
Sbjct: 162 KNGKAVIVGGGYIGLELGAALKINNLDVTMVYPEPWCMPRLFTAGIAAFYEGFYKNKGIN 221

Query: 181 IIKGTVAVGFTSDSNGEVKQVKLKDGRVLDADIVVVGVGGRPLISLFKGQVEEEKGGIKT 240
           IIKGTVAVGFTSDSNGEVKQV+LKDGR LDADIVVVGVGGRPLISLFKGQVEEEKGGIKT
Sbjct: 222 IIKGTVAVGFTSDSNGEVKQVELKDGRSLDADIVVVGVGGRPLISLFKGQVEEEKGGIKT 281

Query: 241 DGFFKTSIPGVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAQEEGKSTDVY 300
           DGFFKTSIP VYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVK IKA+EEG S + Y
Sbjct: 282 DGFFKTSIPDVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKVIKAKEEGNSIEEY 341

Query: 301 DYLPYFYSRAFNLSWQFYGDNVGETILFGDNNPESSQPKFGTYWVKDGKVVGVFLEGGSA 360
           DYLPYFYSR+FNLSWQFYGDNVGE ILFGDNNPES++PKFGTYWVKDGKVVGVFLEGG+A
Sbjct: 342 DYLPYFYSRSFNLSWQFYGDNVGEAILFGDNNPESAEPKFGTYWVKDGKVVGVFLEGGTA 401

Query: 361 DEYKAIAKLARVQPSVQSLDQLAKEGLSFASKV 394
           DEYKAIAKLARVQPSV+SLDQLAKEGLSFASKV
Sbjct: 402 DEYKAIAKLARVQPSVESLDQLAKEGLSFASKV 434

BLAST of MS019549 vs. NCBI nr
Match: XP_023526834.1 (monodehydroascorbate reductase, seedling isozyme-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 743.4 bits (1918), Expect = 9.9e-211
Identity = 367/393 (93.38%), Postives = 385/393 (97.96%), Query Frame = 0

Query: 1   VAPYERPALSKGYLFPESPARLPGFHVCVGSGGEKLLPEWYKEKGIELILSTEIVKADLA 60
           VAPYERPALSKGYLFPESPARLPGFHVCVGSGGE+LLPEWYKE GIELILSTEIVKADLA
Sbjct: 42  VAPYERPALSKGYLFPESPARLPGFHVCVGSGGERLLPEWYKENGIELILSTEIVKADLA 101

Query: 61  AKALTSASGQTFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK 120
           AKAL+++SG +FKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK
Sbjct: 102 AKALSTSSGDSFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK 161

Query: 121 KSGKAVIVGGGYIGLELGAALQMNNLDVTMVYPEPWCMPRLFTSGIAAFYEGYYKNKGIN 180
           K+GKAVIVGGGYIGLELGAAL++NNLDVTMVYPEPWCMPRLFT+GIAAFYEG+YKNKGIN
Sbjct: 162 KNGKAVIVGGGYIGLELGAALKINNLDVTMVYPEPWCMPRLFTAGIAAFYEGFYKNKGIN 221

Query: 181 IIKGTVAVGFTSDSNGEVKQVKLKDGRVLDADIVVVGVGGRPLISLFKGQVEEEKGGIKT 240
           IIKGTVAVGFTSDSNGEVKQV+LKDGR LDADIVVVGVGGRPLISLFKGQVEEEKGGIKT
Sbjct: 222 IIKGTVAVGFTSDSNGEVKQVELKDGRSLDADIVVVGVGGRPLISLFKGQVEEEKGGIKT 281

Query: 241 DGFFKTSIPGVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAQEEGKSTDVY 300
           DGFFKTSIP VYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKA+EEG S + Y
Sbjct: 282 DGFFKTSIPDVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAKEEGNSIEEY 341

Query: 301 DYLPYFYSRAFNLSWQFYGDNVGETILFGDNNPESSQPKFGTYWVKDGKVVGVFLEGGSA 360
           DYLPYFYSR+FNLSWQFYGDNVGE ILFGDNNPES++PKFGTYWVKDGKVVGVFLEGG+A
Sbjct: 342 DYLPYFYSRSFNLSWQFYGDNVGEAILFGDNNPESAEPKFGTYWVKDGKVVGVFLEGGTA 401

Query: 361 DEYKAIAKLARVQPSVQSLDQLAKEGLSFASKV 394
           DEYKAIAKLARVQPSV+SLDQLAKEGLSFASKV
Sbjct: 402 DEYKAIAKLARVQPSVESLDQLAKEGLSFASKV 434

BLAST of MS019549 vs. NCBI nr
Match: XP_038904751.1 (monodehydroascorbate reductase, seedling isozyme-like [Benincasa hispida])

HSP 1 Score: 734.6 bits (1895), Expect = 4.6e-208
Identity = 363/393 (92.37%), Postives = 378/393 (96.18%), Query Frame = 0

Query: 1   VAPYERPALSKGYLFPESPARLPGFHVCVGSGGEKLLPEWYKEKGIELILSTEIVKADLA 60
           VAPYERPALSKGYLFPESPARLPGFHVCVGSGGE+LLPEWYKE GIELILSTEIV ADLA
Sbjct: 42  VAPYERPALSKGYLFPESPARLPGFHVCVGSGGERLLPEWYKENGIELILSTEIVNADLA 101

Query: 61  AKALTSASGQTFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK 120
           AKAL SA G T+KYQILIIATGSTVIRLSDFGV+GADAKNIFYLREVDDADKLVEAIK+K
Sbjct: 102 AKALISACGDTYKYQILIIATGSTVIRLSDFGVEGADAKNIFYLREVDDADKLVEAIKSK 161

Query: 121 KSGKAVIVGGGYIGLELGAALQMNNLDVTMVYPEPWCMPRLFTSGIAAFYEGYYKNKGIN 180
           K+GKAVIVGGGYIGLELGAALQ+NNLDVTMVYPEPWCMPRLFTSGIA+FYEG+YKNKGIN
Sbjct: 162 KNGKAVIVGGGYIGLELGAALQINNLDVTMVYPEPWCMPRLFTSGIASFYEGFYKNKGIN 221

Query: 181 IIKGTVAVGFTSDSNGEVKQVKLKDGRVLDADIVVVGVGGRPLISLFKGQVEEEKGGIKT 240
           IIKGTVA GFTS+S+GEVKQVKLKDGR+LDADIVVVGVGGRPLISLFKGQVEEEKGGIKT
Sbjct: 222 IIKGTVATGFTSNSDGEVKQVKLKDGRILDADIVVVGVGGRPLISLFKGQVEEEKGGIKT 281

Query: 241 DGFFKTSIPGVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAQEEGKSTDVY 300
           DGFFKTSIP VYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKA+EEGKS + Y
Sbjct: 282 DGFFKTSIPDVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAEEEGKSIEEY 341

Query: 301 DYLPYFYSRAFNLSWQFYGDNVGETILFGDNNPESSQPKFGTYWVKDGKVVGVFLEGGSA 360
           DYLPYFYSRAF LSWQFYGDNVGETILFGDNNPES QPKFGTYWVKDGKVVG FLEGG+ 
Sbjct: 342 DYLPYFYSRAFTLSWQFYGDNVGETILFGDNNPESEQPKFGTYWVKDGKVVGAFLEGGTP 401

Query: 361 DEYKAIAKLARVQPSVQSLDQLAKEGLSFASKV 394
           DEYKAIAKLAR QPSV+SLDQLAKEGL FASKV
Sbjct: 402 DEYKAIAKLARDQPSVESLDQLAKEGLCFASKV 434

BLAST of MS019549 vs. ExPASy Swiss-Prot
Match: Q42711 (Monodehydroascorbate reductase, seedling isozyme OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 664.5 bits (1713), Expect = 7.6e-190
Identity = 322/393 (81.93%), Postives = 359/393 (91.35%), Query Frame = 0

Query: 1   VAPYERPALSKGYLFPESPARLPGFHVCVGSGGEKLLPEWYKEKGIELILSTEIVKADLA 60
           VAPYERPALSK YLFPESPARLPGFHVCVGSGGE+LLP+WYKEKGIELILSTEIV+ADL 
Sbjct: 42  VAPYERPALSKAYLFPESPARLPGFHVCVGSGGERLLPDWYKEKGIELILSTEIVEADLP 101

Query: 61  AKALTSASGQTFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK 120
           AK L SA G+ + YQ LIIATGSTVI+LSDFGVQGADAKNIFYLRE+DDAD+LVEAIKAK
Sbjct: 102 AKRLRSAHGKIYNYQTLIIATGSTVIKLSDFGVQGADAKNIFYLREIDDADQLVEAIKAK 161

Query: 121 KSGKAVIVGGGYIGLELGAALQMNNLDVTMVYPEPWCMPRLFTSGIAAFYEGYYKNKGIN 180
           ++GK V+VGGGYIGLELGAAL++NN DV+MVYPEPWCMPRLFT  IAAFYEGYY  KGI 
Sbjct: 162 ENGKVVVVGGGYIGLELGAALRINNFDVSMVYPEPWCMPRLFTPEIAAFYEGYYAQKGIT 221

Query: 181 IIKGTVAVGFTSDSNGEVKQVKLKDGRVLDADIVVVGVGGRPLISLFKGQVEEEKGGIKT 240
           IIKGTVAVGFT D+NGEVK+VKLKDGRVL+ADIVVVGVG RPL SLFKGQ+ EEKGGIKT
Sbjct: 222 IIKGTVAVGFTVDTNGEVKEVKLKDGRVLEADIVVVGVGARPLTSLFKGQIVEEKGGIKT 281

Query: 241 DGFFKTSIPGVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAQEEGKSTDVY 300
           D FFKTS+P VYAVGDVAT+PLKLYNELRRVEHVDH+RKSAEQAVKAIKA EEGK+ + Y
Sbjct: 282 DEFFKTSVPDVYAVGDVATFPLKLYNELRRVEHVDHSRKSAEQAVKAIKASEEGKAIEEY 341

Query: 301 DYLPYFYSRAFNLSWQFYGDNVGETILFGDNNPESSQPKFGTYWVKDGKVVGVFLEGGSA 360
           DYLPYFYSR+F+LSWQFYGDNVG+ +LFGDN+P+S+  KFG+YW+KDGKVVG FLE GS 
Sbjct: 342 DYLPYFYSRSFDLSWQFYGDNVGDAVLFGDNSPDSATHKFGSYWIKDGKVVGAFLESGSP 401

Query: 361 DEYKAIAKLARVQPSVQSLDQLAKEGLSFASKV 394
           +E KAIAK+AR+QPSV+S D L KEG+SFASKV
Sbjct: 402 EENKAIAKVARIQPSVESSDLLLKEGISFASKV 434

BLAST of MS019549 vs. ExPASy Swiss-Prot
Match: Q43497 (Monodehydroascorbate reductase OS=Solanum lycopersicum OX=4081 GN=AFRR PE=1 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 3.5e-187
Identity = 314/393 (79.90%), Postives = 357/393 (90.84%), Query Frame = 0

Query: 1   VAPYERPALSKGYLFPESPARLPGFHVCVGSGGEKLLPEWYKEKGIELILSTEIVKADLA 60
           VAPYERPALSK YLFPE  ARLPGFHVCVGSGGE+ LPEWY EKGI LILSTEIVKADLA
Sbjct: 42  VAPYERPALSKAYLFPEGAARLPGFHVCVGSGGERQLPEWYAEKGISLILSTEIVKADLA 101

Query: 61  AKALTSASGQTFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK 120
           +K L SA+G++FKYQ L+IATG+TV++LSDFGVQGAD+KNIFYLRE+DDAD+LVEA+KAK
Sbjct: 102 SKTLVSAAGESFKYQTLVIATGTTVLKLSDFGVQGADSKNIFYLREIDDADQLVEALKAK 161

Query: 121 KSGKAVIVGGGYIGLELGAALQMNNLDVTMVYPEPWCMPRLFTSGIAAFYEGYYKNKGIN 180
           K+GKAV+VGGGYIGLEL A L++NN++V MVYPEPWCMPRLFT GIAAFYEGYYKNKG+N
Sbjct: 162 KNGKAVVVGGGYIGLELSAVLRLNNIEVNMVYPEPWCMPRLFTEGIAAFYEGYYKNKGVN 221

Query: 181 IIKGTVAVGFTSDSNGEVKQVKLKDGRVLDADIVVVGVGGRPLISLFKGQVEEEKGGIKT 240
           IIKGTVAVGF +  NGEVK+VKLKDGRVL+ADIVVVGVG RPL +LFKGQVEEEKGGIKT
Sbjct: 222 IIKGTVAVGFDTHPNGEVKEVKLKDGRVLEADIVVVGVGARPLTTLFKGQVEEEKGGIKT 281

Query: 241 DGFFKTSIPGVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAQEEGKSTDVY 300
           D FFKTS+P VYAVGDVAT+PLK+YNE+RRVEHVDH+RKSAEQAVKAI A E+GKS D Y
Sbjct: 282 DAFFKTSVPDVYAVGDVATFPLKMYNEIRRVEHVDHSRKSAEQAVKAIFASEQGKSVDEY 341

Query: 301 DYLPYFYSRAFNLSWQFYGDNVGETILFGDNNPESSQPKFGTYWVKDGKVVGVFLEGGSA 360
           DYLPYFYSRAF+LSWQFYGDNVGET+LFGD +P S+  KFG YW+KDGK+VG FLE GS 
Sbjct: 342 DYLPYFYSRAFDLSWQFYGDNVGETVLFGDADPNSATHKFGQYWIKDGKIVGAFLESGSP 401

Query: 361 DEYKAIAKLARVQPSVQSLDQLAKEGLSFASKV 394
           +E KAIAK+A+VQP   +LDQLA+EG+SFASK+
Sbjct: 402 EENKAIAKVAKVQPPA-TLDQLAQEGISFASKI 433

BLAST of MS019549 vs. ExPASy Swiss-Prot
Match: Q40977 (Monodehydroascorbate reductase OS=Pisum sativum OX=3888 PE=1 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 6.0e-187
Identity = 316/393 (80.41%), Postives = 357/393 (90.84%), Query Frame = 0

Query: 1   VAPYERPALSKGYLFPESPARLPGFHVCVGSGGEKLLPEWYKEKGIELILSTEIVKADLA 60
           VAPYERPALSK YLFPESPARLPGFH CVGSGGE+LLPEWY EKGI+L LSTEIV ADLA
Sbjct: 41  VAPYERPALSKAYLFPESPARLPGFHTCVGSGGERLLPEWYSEKGIQLYLSTEIVSADLA 100

Query: 61  AKALTSASGQTFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK 120
           AK L SA+G+ F YQ L+IATGS VIRL+DFGV GA+AKNIFYLREVDDADKL EAIK K
Sbjct: 101 AKFLKSANGEHFDYQTLVIATGSAVIRLTDFGVIGANAKNIFYLREVDDADKLYEAIKRK 160

Query: 121 KSGKAVIVGGGYIGLELGAALQMNNLDVTMVYPEPWCMPRLFTSGIAAFYEGYYKNKGIN 180
           K+ K V+VGGGYIGLEL A L++N+LDVTMVYPEPWCMPRLFTS IAAFYEGYY NKGIN
Sbjct: 161 KNAKRVVVGGGYIGLELSAVLKLNDLDVTMVYPEPWCMPRLFTSEIAAFYEGYYANKGIN 220

Query: 181 IIKGTVAVGFTSDSNGEVKQVKLKDGRVLDADIVVVGVGGRPLISLFKGQVEEEKGGIKT 240
           IIKGTVAVGFT++S+GEVK+VKLKDGRVL+ADIV+VGVGGRP ISLFKGQVEE+ GGIKT
Sbjct: 221 IIKGTVAVGFTANSDGEVKEVKLKDGRVLEADIVIVGVGGRPQISLFKGQVEEQHGGIKT 280

Query: 241 DGFFKTSIPGVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAQEEGKSTDVY 300
           D FFKTS+P VYAVGDVAT+PLKLYN++RRVEHVDHARKSAEQA KAI A + GKS + Y
Sbjct: 281 DSFFKTSVPDVYAVGDVATFPLKLYNDVRRVEHVDHARKSAEQAAKAIFAADVGKSVEEY 340

Query: 301 DYLPYFYSRAFNLSWQFYGDNVGETILFGDNNPESSQPKFGTYWVKDGKVVGVFLEGGSA 360
           DYLPYFYSR+F+LSWQFYGDNVGET+LFGDN+P SS+PKFGTYW+K+GKVVG FLEGG+ 
Sbjct: 341 DYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTP 400

Query: 361 DEYKAIAKLARVQPSVQSLDQLAKEGLSFASKV 394
           DE KAIAK+AR +P+V+ ++QLA+EGLSFASK+
Sbjct: 401 DENKAIAKVARAKPAVEDVNQLAEEGLSFASKI 433

BLAST of MS019549 vs. ExPASy Swiss-Prot
Match: Q652L6 (Monodehydroascorbate reductase 3, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=MDAR3 PE=1 SV=1)

HSP 1 Score: 642.1 bits (1655), Expect = 4.1e-183
Identity = 307/393 (78.12%), Postives = 353/393 (89.82%), Query Frame = 0

Query: 1   VAPYERPALSKGYLFPESPARLPGFHVCVGSGGEKLLPEWYKEKGIELILSTEIVKADLA 60
           VAPYERPALSKGYLFP++ ARLPGFHVCVGSGGE+LLPEWY EKGIELILSTEIVKADLA
Sbjct: 43  VAPYERPALSKGYLFPQNAARLPGFHVCVGSGGERLLPEWYSEKGIELILSTEIVKADLA 102

Query: 61  AKALTSASGQTFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK 120
           +K LTSA G TF Y+ILIIATGS+VI+LSDFG QGAD+ NI YLREVDDADKLV AI+AK
Sbjct: 103 SKTLTSAVGATFTYEILIIATGSSVIKLSDFGTQGADSNNILYLREVDDADKLVAAIQAK 162

Query: 121 KSGKAVIVGGGYIGLELGAALQMNNLDVTMVYPEPWCMPRLFTSGIAAFYEGYYKNKGIN 180
           K GKAVIVGGGYIGLEL AAL++N+ DVTMV+PEPWCMPRLFT+ IAAFYE YY NKG+ 
Sbjct: 163 KGGKAVIVGGGYIGLELSAALKINDFDVTMVFPEPWCMPRLFTADIAAFYESYYTNKGVK 222

Query: 181 IIKGTVAVGFTSDSNGEVKQVKLKDGRVLDADIVVVGVGGRPLISLFKGQVEEEKGGIKT 240
           I+KGTVAVGF +D+NG+V  V LK+G VL+ADIVVVGVGGRPL +LFKGQV EEKGGIKT
Sbjct: 223 IVKGTVAVGFDADANGDVTAVNLKNGSVLEADIVVVGVGGRPLTTLFKGQVAEEKGGIKT 282

Query: 241 DGFFKTSIPGVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAQEEGKSTDVY 300
           D FF+TS+PGVYAVGDVAT+P+K+YNELRRVEHVDHARKSAEQAVKAIK +E G+S   Y
Sbjct: 283 DAFFETSVPGVYAVGDVATFPMKMYNELRRVEHVDHARKSAEQAVKAIKGKESGESVVEY 342

Query: 301 DYLPYFYSRAFNLSWQFYGDNVGETILFGDNNPESSQPKFGTYWVKDGKVVGVFLEGGSA 360
           DYLPYFYSR+F+L WQFYGDNVG+TILFGD++P S++PKFG+YW+KDGKV+G FLEGGS 
Sbjct: 343 DYLPYFYSRSFDLGWQFYGDNVGDTILFGDSDPTSAKPKFGSYWIKDGKVLGAFLEGGSP 402

Query: 361 DEYKAIAKLARVQPSVQSLDQLAKEGLSFASKV 394
           DE KAIAK+A+ QP V ++++L KEGL FASK+
Sbjct: 403 DENKAIAKVAKTQPPVANIEELKKEGLQFASKI 435

BLAST of MS019549 vs. ExPASy Swiss-Prot
Match: Q9LFA3 (Monodehydroascorbate reductase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=MDAR1 PE=1 SV=1)

HSP 1 Score: 636.7 bits (1641), Expect = 1.7e-181
Identity = 301/393 (76.59%), Postives = 353/393 (89.82%), Query Frame = 0

Query: 1   VAPYERPALSKGYLFPESPARLPGFHVCVGSGGEKLLPEWYKEKGIELILSTEIVKADLA 60
           VAPYERPALSKGYLFPE  ARLPGFH CVGSGGEKLLPE YK+KGIELILSTEIVKADL+
Sbjct: 42  VAPYERPALSKGYLFPEGAARLPGFHCCVGSGGEKLLPESYKQKGIELILSTEIVKADLS 101

Query: 61  AKALTSASGQTFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK 120
           AK+L SA+G  FKYQ LIIATGSTV+RL+DFGV+GAD+KNI YLRE+DDADKLVEAIKAK
Sbjct: 102 AKSLVSATGDVFKYQTLIIATGSTVLRLTDFGVKGADSKNILYLREIDDADKLVEAIKAK 161

Query: 121 KSGKAVIVGGGYIGLELGAALQMNNLDVTMVYPEPWCMPRLFTSGIAAFYEGYYKNKGIN 180
           K GKAV+VGGGYIGLEL A L++NNLDVTMV+PEPWCMPRLFT+ IAAFYE YY NKG+ 
Sbjct: 162 KGGKAVVVGGGYIGLELSAVLRINNLDVTMVFPEPWCMPRLFTADIAAFYETYYTNKGVK 221

Query: 181 IIKGTVAVGFTSDSNGEVKQVKLKDGRVLDADIVVVGVGGRPLISLFKGQVEEEKGGIKT 240
           IIKGTVA GFT+  NGEVK+V+LKDGR L+ADIV+VGVG +PL SLFKGQVEE+KGGIKT
Sbjct: 222 IIKGTVASGFTAQPNGEVKEVQLKDGRTLEADIVIVGVGAKPLTSLFKGQVEEDKGGIKT 281

Query: 241 DGFFKTSIPGVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAQEEGKSTDVY 300
           D FFKTS+P VYAVGDVAT+PLK+Y ++RRVEHVDH+RKSAEQAVKAIKA E G + + Y
Sbjct: 282 DAFFKTSVPDVYAVGDVATFPLKMYGDVRRVEHVDHSRKSAEQAVKAIKAAEGGAAVEEY 341

Query: 301 DYLPYFYSRAFNLSWQFYGDNVGETILFGDNNPESSQPKFGTYWVKDGKVVGVFLEGGSA 360
           DYLP+FYSR+F+LSWQFYGDNVG+++LFGD+NP + +P+FG YWV+ GKVVG F+EGGS 
Sbjct: 342 DYLPFFYSRSFDLSWQFYGDNVGDSVLFGDSNPSNPKPRFGAYWVQGGKVVGAFMEGGSG 401

Query: 361 DEYKAIAKLARVQPSVQSLDQLAKEGLSFASKV 394
           DE KA+AK+A+ +PS +SLD+L K+G+SFA+K+
Sbjct: 402 DENKALAKVAKARPSAESLDELVKQGISFAAKI 434

BLAST of MS019549 vs. ExPASy TrEMBL
Match: A0A6J1CSV7 (monodehydroascorbate reductase, seedling isozyme-like OS=Momordica charantia OX=3673 GN=LOC111014281 PE=4 SV=1)

HSP 1 Score: 784.6 bits (2025), Expect = 1.9e-223
Identity = 393/393 (100.00%), Postives = 393/393 (100.00%), Query Frame = 0

Query: 1   VAPYERPALSKGYLFPESPARLPGFHVCVGSGGEKLLPEWYKEKGIELILSTEIVKADLA 60
           VAPYERPALSKGYLFPESPARLPGFHVCVGSGGEKLLPEWYKEKGIELILSTEIVKADLA
Sbjct: 42  VAPYERPALSKGYLFPESPARLPGFHVCVGSGGEKLLPEWYKEKGIELILSTEIVKADLA 101

Query: 61  AKALTSASGQTFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK 120
           AKALTSASGQTFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK
Sbjct: 102 AKALTSASGQTFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK 161

Query: 121 KSGKAVIVGGGYIGLELGAALQMNNLDVTMVYPEPWCMPRLFTSGIAAFYEGYYKNKGIN 180
           KSGKAVIVGGGYIGLELGAALQMNNLDVTMVYPEPWCMPRLFTSGIAAFYEGYYKNKGIN
Sbjct: 162 KSGKAVIVGGGYIGLELGAALQMNNLDVTMVYPEPWCMPRLFTSGIAAFYEGYYKNKGIN 221

Query: 181 IIKGTVAVGFTSDSNGEVKQVKLKDGRVLDADIVVVGVGGRPLISLFKGQVEEEKGGIKT 240
           IIKGTVAVGFTSDSNGEVKQVKLKDGRVLDADIVVVGVGGRPLISLFKGQVEEEKGGIKT
Sbjct: 222 IIKGTVAVGFTSDSNGEVKQVKLKDGRVLDADIVVVGVGGRPLISLFKGQVEEEKGGIKT 281

Query: 241 DGFFKTSIPGVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAQEEGKSTDVY 300
           DGFFKTSIPGVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAQEEGKSTDVY
Sbjct: 282 DGFFKTSIPGVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAQEEGKSTDVY 341

Query: 301 DYLPYFYSRAFNLSWQFYGDNVGETILFGDNNPESSQPKFGTYWVKDGKVVGVFLEGGSA 360
           DYLPYFYSRAFNLSWQFYGDNVGETILFGDNNPESSQPKFGTYWVKDGKVVGVFLEGGSA
Sbjct: 342 DYLPYFYSRAFNLSWQFYGDNVGETILFGDNNPESSQPKFGTYWVKDGKVVGVFLEGGSA 401

Query: 361 DEYKAIAKLARVQPSVQSLDQLAKEGLSFASKV 394
           DEYKAIAKLARVQPSVQSLDQLAKEGLSFASKV
Sbjct: 402 DEYKAIAKLARVQPSVQSLDQLAKEGLSFASKV 434

BLAST of MS019549 vs. ExPASy TrEMBL
Match: A0A6J1F265 (monodehydroascorbate reductase, seedling isozyme-like OS=Cucurbita moschata OX=3662 GN=LOC111441711 PE=4 SV=1)

HSP 1 Score: 745.0 bits (1922), Expect = 1.6e-211
Identity = 368/393 (93.64%), Postives = 385/393 (97.96%), Query Frame = 0

Query: 1   VAPYERPALSKGYLFPESPARLPGFHVCVGSGGEKLLPEWYKEKGIELILSTEIVKADLA 60
           VAPYERPALSKGYLFPESPARLPGFHVCVGSGGE+LLPEWYKE GIELILSTEIVKADLA
Sbjct: 42  VAPYERPALSKGYLFPESPARLPGFHVCVGSGGERLLPEWYKENGIELILSTEIVKADLA 101

Query: 61  AKALTSASGQTFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK 120
           AKAL+++SG TFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK
Sbjct: 102 AKALSTSSGDTFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK 161

Query: 121 KSGKAVIVGGGYIGLELGAALQMNNLDVTMVYPEPWCMPRLFTSGIAAFYEGYYKNKGIN 180
           K+GKAVIVGGGYIGLELGAAL++NNLDVTMVYPEPWCMPRLFT+GIAAFYEG+YKNKGIN
Sbjct: 162 KNGKAVIVGGGYIGLELGAALKINNLDVTMVYPEPWCMPRLFTAGIAAFYEGFYKNKGIN 221

Query: 181 IIKGTVAVGFTSDSNGEVKQVKLKDGRVLDADIVVVGVGGRPLISLFKGQVEEEKGGIKT 240
           IIKGTVAVGFTSDSNGEVKQV+LKDGR LDADIVVVGVGGRPLISLFKGQVEEEKGGIKT
Sbjct: 222 IIKGTVAVGFTSDSNGEVKQVELKDGRSLDADIVVVGVGGRPLISLFKGQVEEEKGGIKT 281

Query: 241 DGFFKTSIPGVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAQEEGKSTDVY 300
           DGFFKTSIP VYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKA+EEG S + Y
Sbjct: 282 DGFFKTSIPDVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAKEEGNSIEEY 341

Query: 301 DYLPYFYSRAFNLSWQFYGDNVGETILFGDNNPESSQPKFGTYWVKDGKVVGVFLEGGSA 360
           DYLPYFYSR+FNLSWQFYGDNVGE ILFGDNNPES++PKFGTYWVKDGKVVGVFLEGG+A
Sbjct: 342 DYLPYFYSRSFNLSWQFYGDNVGEAILFGDNNPESAEPKFGTYWVKDGKVVGVFLEGGTA 401

Query: 361 DEYKAIAKLARVQPSVQSLDQLAKEGLSFASKV 394
           DEYKAIAKLARVQPSV+SLDQLAKEGLSFASKV
Sbjct: 402 DEYKAIAKLARVQPSVESLDQLAKEGLSFASKV 434

BLAST of MS019549 vs. ExPASy TrEMBL
Match: A0A6J1J6E3 (monodehydroascorbate reductase, seedling isozyme-like OS=Cucurbita maxima OX=3661 GN=LOC111481646 PE=4 SV=1)

HSP 1 Score: 743.4 bits (1918), Expect = 4.8e-211
Identity = 367/393 (93.38%), Postives = 384/393 (97.71%), Query Frame = 0

Query: 1   VAPYERPALSKGYLFPESPARLPGFHVCVGSGGEKLLPEWYKEKGIELILSTEIVKADLA 60
           VAPYERPALSKGYLFPESPARLPGFHVCVGSGGE+LLPEWYKE GIELILSTEIVKADLA
Sbjct: 42  VAPYERPALSKGYLFPESPARLPGFHVCVGSGGERLLPEWYKENGIELILSTEIVKADLA 101

Query: 61  AKALTSASGQTFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK 120
           AKAL+++SG TFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK
Sbjct: 102 AKALSTSSGDTFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK 161

Query: 121 KSGKAVIVGGGYIGLELGAALQMNNLDVTMVYPEPWCMPRLFTSGIAAFYEGYYKNKGIN 180
           K+GKAVIVGGGYIGLELGAAL++NNLDVTMVYPEPWCMPRLFT+GIAAFYEG+YKNKGIN
Sbjct: 162 KNGKAVIVGGGYIGLELGAALKINNLDVTMVYPEPWCMPRLFTAGIAAFYEGFYKNKGIN 221

Query: 181 IIKGTVAVGFTSDSNGEVKQVKLKDGRVLDADIVVVGVGGRPLISLFKGQVEEEKGGIKT 240
           IIKGTVAVGFTSDSNGEVKQV+LKDGR LDADIVVVGVGGRPLISLFKGQVEEEKGGIKT
Sbjct: 222 IIKGTVAVGFTSDSNGEVKQVELKDGRSLDADIVVVGVGGRPLISLFKGQVEEEKGGIKT 281

Query: 241 DGFFKTSIPGVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAQEEGKSTDVY 300
           DGFFKTSIP VYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVK IKA+EEG S + Y
Sbjct: 282 DGFFKTSIPDVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKVIKAKEEGNSIEEY 341

Query: 301 DYLPYFYSRAFNLSWQFYGDNVGETILFGDNNPESSQPKFGTYWVKDGKVVGVFLEGGSA 360
           DYLPYFYSR+FNLSWQFYGDNVGE ILFGDNNPES++PKFGTYWVKDGKVVGVFLEGG+A
Sbjct: 342 DYLPYFYSRSFNLSWQFYGDNVGEAILFGDNNPESAEPKFGTYWVKDGKVVGVFLEGGTA 401

Query: 361 DEYKAIAKLARVQPSVQSLDQLAKEGLSFASKV 394
           DEYKAIAKLARVQPSV+SLDQLAKEGLSFASKV
Sbjct: 402 DEYKAIAKLARVQPSVESLDQLAKEGLSFASKV 434

BLAST of MS019549 vs. ExPASy TrEMBL
Match: A0A0A0LGL9 (Pyr_redox_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G775240 PE=4 SV=1)

HSP 1 Score: 722.2 bits (1863), Expect = 1.1e-204
Identity = 349/393 (88.80%), Postives = 379/393 (96.44%), Query Frame = 0

Query: 1   VAPYERPALSKGYLFPESPARLPGFHVCVGSGGEKLLPEWYKEKGIELILSTEIVKADLA 60
           VAPYERPALSK YLFPESPARLPGFHVCVGSGG++LLP WYKE GIELILSTEIVKADLA
Sbjct: 42  VAPYERPALSKAYLFPESPARLPGFHVCVGSGGQRLLPGWYKENGIELILSTEIVKADLA 101

Query: 61  AKALTSASGQTFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK 120
           AK+LT+ASG+TFKYQIL+IATGSTVI+LSDFGV+GADAKNIFYLRE+ DAD+LVEAIK+K
Sbjct: 102 AKSLTTASGETFKYQILVIATGSTVIKLSDFGVEGADAKNIFYLREIADADQLVEAIKSK 161

Query: 121 KSGKAVIVGGGYIGLELGAALQMNNLDVTMVYPEPWCMPRLFTSGIAAFYEGYYKNKGIN 180
           K+GKAV+VGGGYIGLELGAAL++N+LDVTM+YPEPWCMPRLFTSGIA+FYEG+YKNKGIN
Sbjct: 162 KNGKAVLVGGGYIGLELGAALKINDLDVTMIYPEPWCMPRLFTSGIASFYEGFYKNKGIN 221

Query: 181 IIKGTVAVGFTSDSNGEVKQVKLKDGRVLDADIVVVGVGGRPLISLFKGQVEEEKGGIKT 240
           IIKGTVA GFTSDSNGEVKQVKLKDGR LDADIVVVGVGGRPL+SLFKGQVEE+KGGIKT
Sbjct: 222 IIKGTVATGFTSDSNGEVKQVKLKDGRTLDADIVVVGVGGRPLVSLFKGQVEEDKGGIKT 281

Query: 241 DGFFKTSIPGVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAQEEGKSTDVY 300
           DGFFKTSIP VYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAQEEGKS + Y
Sbjct: 282 DGFFKTSIPDVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAQEEGKSIEEY 341

Query: 301 DYLPYFYSRAFNLSWQFYGDNVGETILFGDNNPESSQPKFGTYWVKDGKVVGVFLEGGSA 360
           DYLPYFYSR FNL+WQFYGDNVGET+LFGDNNPES +PKFGTYW+KDGKVVGVFLEGG+ 
Sbjct: 342 DYLPYFYSRTFNLAWQFYGDNVGETVLFGDNNPESPKPKFGTYWIKDGKVVGVFLEGGTP 401

Query: 361 DEYKAIAKLARVQPSVQSLDQLAKEGLSFASKV 394
           DEYKAIAK+ARVQP V+SLDQLAK+GL FASKV
Sbjct: 402 DEYKAIAKVARVQPPVESLDQLAKDGLCFASKV 434

BLAST of MS019549 vs. ExPASy TrEMBL
Match: A0A5D3DNM4 (Monodehydroascorbate reductase, seedling isozyme-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G003660 PE=4 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 6.3e-203
Identity = 349/393 (88.80%), Postives = 377/393 (95.93%), Query Frame = 0

Query: 1   VAPYERPALSKGYLFPESPARLPGFHVCVGSGGEKLLPEWYKEKGIELILSTEIVKADLA 60
           VAPYERPALSK YLFPESPARLPGFHVCVGSGGE+LLP WYKE GIELILSTEIVKADLA
Sbjct: 42  VAPYERPALSKAYLFPESPARLPGFHVCVGSGGERLLPGWYKENGIELILSTEIVKADLA 101

Query: 61  AKALTSASGQTFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK 120
           AKALT+A G TF+YQ LIIATGSTVI+LSDFGV+GADAKNIFYLRE+ DAD+LVEAIK+K
Sbjct: 102 AKALTTACGATFEYQNLIIATGSTVIKLSDFGVEGADAKNIFYLREIADADQLVEAIKSK 161

Query: 121 KSGKAVIVGGGYIGLELGAALQMNNLDVTMVYPEPWCMPRLFTSGIAAFYEGYYKNKGIN 180
           K+GKAVIVGGGYIGLELGAAL++N+LDVTMVYPEPWCMPRLFTSGIAAFYEG+YKNKGIN
Sbjct: 162 KNGKAVIVGGGYIGLELGAALKINDLDVTMVYPEPWCMPRLFTSGIAAFYEGFYKNKGIN 221

Query: 181 IIKGTVAVGFTSDSNGEVKQVKLKDGRVLDADIVVVGVGGRPLISLFKGQVEEEKGGIKT 240
           IIKGTVA GFT+DSNGEVKQVKLKDGR LDADIVVVGVGGRPL+SLFKGQVEE+KGGIKT
Sbjct: 222 IIKGTVATGFTTDSNGEVKQVKLKDGRTLDADIVVVGVGGRPLVSLFKGQVEEDKGGIKT 281

Query: 241 DGFFKTSIPGVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAQEEGKSTDVY 300
           DGFFKTSIP VYAVGDVAT+PLKLYNELRRVEHVDHARKSAEQAVKAIKA+EEGKS + Y
Sbjct: 282 DGFFKTSIPDVYAVGDVATFPLKLYNELRRVEHVDHARKSAEQAVKAIKAKEEGKSIEEY 341

Query: 301 DYLPYFYSRAFNLSWQFYGDNVGETILFGDNNPESSQPKFGTYWVKDGKVVGVFLEGGSA 360
           DYLPYFYSR+FNL+WQFYGDNVGET+LFGDNNPES++PKFGTYW+KDGKVVG FLEGG+ 
Sbjct: 342 DYLPYFYSRSFNLAWQFYGDNVGETVLFGDNNPESAKPKFGTYWIKDGKVVGAFLEGGTP 401

Query: 361 DEYKAIAKLARVQPSVQSLDQLAKEGLSFASKV 394
           DEYKAIAKLARVQP V+SLDQLAK+GL FASKV
Sbjct: 402 DEYKAIAKLARVQPPVESLDQLAKDGLCFASKV 434

BLAST of MS019549 vs. TAIR 10
Match: AT3G52880.1 (monodehydroascorbate reductase 1 )

HSP 1 Score: 636.7 bits (1641), Expect = 1.2e-182
Identity = 301/393 (76.59%), Postives = 353/393 (89.82%), Query Frame = 0

Query: 1   VAPYERPALSKGYLFPESPARLPGFHVCVGSGGEKLLPEWYKEKGIELILSTEIVKADLA 60
           VAPYERPALSKGYLFPE  ARLPGFH CVGSGGEKLLPE YK+KGIELILSTEIVKADL+
Sbjct: 42  VAPYERPALSKGYLFPEGAARLPGFHCCVGSGGEKLLPESYKQKGIELILSTEIVKADLS 101

Query: 61  AKALTSASGQTFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK 120
           AK+L SA+G  FKYQ LIIATGSTV+RL+DFGV+GAD+KNI YLRE+DDADKLVEAIKAK
Sbjct: 102 AKSLVSATGDVFKYQTLIIATGSTVLRLTDFGVKGADSKNILYLREIDDADKLVEAIKAK 161

Query: 121 KSGKAVIVGGGYIGLELGAALQMNNLDVTMVYPEPWCMPRLFTSGIAAFYEGYYKNKGIN 180
           K GKAV+VGGGYIGLEL A L++NNLDVTMV+PEPWCMPRLFT+ IAAFYE YY NKG+ 
Sbjct: 162 KGGKAVVVGGGYIGLELSAVLRINNLDVTMVFPEPWCMPRLFTADIAAFYETYYTNKGVK 221

Query: 181 IIKGTVAVGFTSDSNGEVKQVKLKDGRVLDADIVVVGVGGRPLISLFKGQVEEEKGGIKT 240
           IIKGTVA GFT+  NGEVK+V+LKDGR L+ADIV+VGVG +PL SLFKGQVEE+KGGIKT
Sbjct: 222 IIKGTVASGFTAQPNGEVKEVQLKDGRTLEADIVIVGVGAKPLTSLFKGQVEEDKGGIKT 281

Query: 241 DGFFKTSIPGVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAQEEGKSTDVY 300
           D FFKTS+P VYAVGDVAT+PLK+Y ++RRVEHVDH+RKSAEQAVKAIKA E G + + Y
Sbjct: 282 DAFFKTSVPDVYAVGDVATFPLKMYGDVRRVEHVDHSRKSAEQAVKAIKAAEGGAAVEEY 341

Query: 301 DYLPYFYSRAFNLSWQFYGDNVGETILFGDNNPESSQPKFGTYWVKDGKVVGVFLEGGSA 360
           DYLP+FYSR+F+LSWQFYGDNVG+++LFGD+NP + +P+FG YWV+ GKVVG F+EGGS 
Sbjct: 342 DYLPFFYSRSFDLSWQFYGDNVGDSVLFGDSNPSNPKPRFGAYWVQGGKVVGAFMEGGSG 401

Query: 361 DEYKAIAKLARVQPSVQSLDQLAKEGLSFASKV 394
           DE KA+AK+A+ +PS +SLD+L K+G+SFA+K+
Sbjct: 402 DENKALAKVAKARPSAESLDELVKQGISFAAKI 434

BLAST of MS019549 vs. TAIR 10
Match: AT3G52880.2 (monodehydroascorbate reductase 1 )

HSP 1 Score: 636.7 bits (1641), Expect = 1.2e-182
Identity = 301/393 (76.59%), Postives = 353/393 (89.82%), Query Frame = 0

Query: 1   VAPYERPALSKGYLFPESPARLPGFHVCVGSGGEKLLPEWYKEKGIELILSTEIVKADLA 60
           VAPYERPALSKGYLFPE  ARLPGFH CVGSGGEKLLPE YK+KGIELILSTEIVKADL+
Sbjct: 74  VAPYERPALSKGYLFPEGAARLPGFHCCVGSGGEKLLPESYKQKGIELILSTEIVKADLS 133

Query: 61  AKALTSASGQTFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK 120
           AK+L SA+G  FKYQ LIIATGSTV+RL+DFGV+GAD+KNI YLRE+DDADKLVEAIKAK
Sbjct: 134 AKSLVSATGDVFKYQTLIIATGSTVLRLTDFGVKGADSKNILYLREIDDADKLVEAIKAK 193

Query: 121 KSGKAVIVGGGYIGLELGAALQMNNLDVTMVYPEPWCMPRLFTSGIAAFYEGYYKNKGIN 180
           K GKAV+VGGGYIGLEL A L++NNLDVTMV+PEPWCMPRLFT+ IAAFYE YY NKG+ 
Sbjct: 194 KGGKAVVVGGGYIGLELSAVLRINNLDVTMVFPEPWCMPRLFTADIAAFYETYYTNKGVK 253

Query: 181 IIKGTVAVGFTSDSNGEVKQVKLKDGRVLDADIVVVGVGGRPLISLFKGQVEEEKGGIKT 240
           IIKGTVA GFT+  NGEVK+V+LKDGR L+ADIV+VGVG +PL SLFKGQVEE+KGGIKT
Sbjct: 254 IIKGTVASGFTAQPNGEVKEVQLKDGRTLEADIVIVGVGAKPLTSLFKGQVEEDKGGIKT 313

Query: 241 DGFFKTSIPGVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAQEEGKSTDVY 300
           D FFKTS+P VYAVGDVAT+PLK+Y ++RRVEHVDH+RKSAEQAVKAIKA E G + + Y
Sbjct: 314 DAFFKTSVPDVYAVGDVATFPLKMYGDVRRVEHVDHSRKSAEQAVKAIKAAEGGAAVEEY 373

Query: 301 DYLPYFYSRAFNLSWQFYGDNVGETILFGDNNPESSQPKFGTYWVKDGKVVGVFLEGGSA 360
           DYLP+FYSR+F+LSWQFYGDNVG+++LFGD+NP + +P+FG YWV+ GKVVG F+EGGS 
Sbjct: 374 DYLPFFYSRSFDLSWQFYGDNVGDSVLFGDSNPSNPKPRFGAYWVQGGKVVGAFMEGGSG 433

Query: 361 DEYKAIAKLARVQPSVQSLDQLAKEGLSFASKV 394
           DE KA+AK+A+ +PS +SLD+L K+G+SFA+K+
Sbjct: 434 DENKALAKVAKARPSAESLDELVKQGISFAAKI 466

BLAST of MS019549 vs. TAIR 10
Match: AT5G03630.1 (Pyridine nucleotide-disulphide oxidoreductase family protein )

HSP 1 Score: 633.6 bits (1633), Expect = 1.0e-181
Identity = 304/393 (77.35%), Postives = 348/393 (88.55%), Query Frame = 0

Query: 1   VAPYERPALSKGYLFPESPARLPGFHVCVGSGGEKLLPEWYKEKGIELILSTEIVKADLA 60
           V PYERPALSKGY+  E+ A LP F+V  G GGE+  P+WYKEKGIELIL TEIVKADLA
Sbjct: 43  VPPYERPALSKGYIHLENKATLPNFYVAAGIGGERQFPQWYKEKGIELILGTEIVKADLA 102

Query: 61  AKALTSASGQTFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKAK 120
           AK L S +GQ FKYQ L+ ATGS+VIRLSDFGV GADAKNIFYLRE++DAD L  A++ K
Sbjct: 103 AKTLVSGTGQVFKYQTLLAATGSSVIRLSDFGVPGADAKNIFYLRELEDADYLAYAMETK 162

Query: 121 KSGKAVIVGGGYIGLELGAALQMNNLDVTMVYPEPWCMPRLFTSGIAAFYEGYYKNKGIN 180
           + GKAV+VGGGYIGLELGAAL+ NNLDVTMVYPEPWCMPRLFT+GIA+FYEGYY NKGIN
Sbjct: 163 EKGKAVVVGGGYIGLELGAALKANNLDVTMVYPEPWCMPRLFTAGIASFYEGYYANKGIN 222

Query: 181 IIKGTVAVGFTSDSNGEVKQVKLKDGRVLDADIVVVGVGGRPLISLFKGQVEEEKGGIKT 240
           I+KGTVA GFT++SNGEV +VKLKDGR L+ADIV+VGVGGRP+ISLFK QVEEEKGG+KT
Sbjct: 223 IVKGTVASGFTTNSNGEVTEVKLKDGRTLEADIVIVGVGGRPIISLFKDQVEEEKGGLKT 282

Query: 241 DGFFKTSIPGVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAQEEGKSTDVY 300
           DGFFKTS+P VYA+GDVAT+P+KLYNE+RRVEHVDHARKSAEQAVKAIKA EEG S   Y
Sbjct: 283 DGFFKTSLPDVYAIGDVATFPMKLYNEMRRVEHVDHARKSAEQAVKAIKAAEEGNSIPEY 342

Query: 301 DYLPYFYSRAFNLSWQFYGDNVGETILFGDNNPESSQPKFGTYWVKDGKVVGVFLEGGSA 360
           DYLPYFYSRAF+LSWQFYGDNVGE++LFGDN+PES +PKFG+YW+K+ KVVG FLEGGS 
Sbjct: 343 DYLPYFYSRAFDLSWQFYGDNVGESVLFGDNDPESPKPKFGSYWIKERKVVGAFLEGGSP 402

Query: 361 DEYKAIAKLARVQPSVQSLDQLAKEGLSFASKV 394
           +E  AIAKLAR QPSV+SL+ L+KEGLSFA+ +
Sbjct: 403 EENNAIAKLARAQPSVESLEVLSKEGLSFATNI 435

BLAST of MS019549 vs. TAIR 10
Match: AT3G09940.1 (monodehydroascorbate reductase )

HSP 1 Score: 542.7 bits (1397), Expect = 2.4e-154
Identity = 264/393 (67.18%), Postives = 325/393 (82.70%), Query Frame = 0

Query: 1   VAPYERPALSKGYLFPESPARLPGFHVCVGSGGEKLLPEWYKEKGIELILSTEIVKADLA 60
           V P+ERP L+K Y+  E    L   +VC G+G  K  P WYKEKGI+LI+ TEIVKADLA
Sbjct: 43  VPPFERPELTKVYIDLEVNPTLANIYVCAGTGEAKQYPNWYKEKGIDLIVGTEIVKADLA 102

Query: 61  AKALTSASGQTFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKA- 120
           +K L S  G+ +KYQ L+IATGST IRLS+ GVQ AD KNIFYLRE++D+D+L  A++  
Sbjct: 103 SKTLVSDDGKIYKYQTLLIATGSTNIRLSEIGVQEADVKNIFYLREIEDSDELALAMELY 162

Query: 121 KKSGKAVIVGGGYIGLELGAALQMNNLDVTMVYPEPWCMPRLFTSGIAAFYEGYYKNKGI 180
            + GKAVI+GGG++GLE+ +AL+ NN +VTMV+PEPW + R FT+ IA+FYE YY NKGI
Sbjct: 163 VQRGKAVIIGGGFLGLEISSALRANNHEVTMVFPEPWLVHRFFTAEIASFYESYYANKGI 222

Query: 181 NIIKGTVAVGFTSDSNGEVKQVKLKDGRVLDADIVVVGVGGRPLISLFKGQVEEEKGGIK 240
            IIKGTVA GF+++S+GEV +VKL+DGR L+A+IVV GVG RP  SLFKGQ+EEEKGGIK
Sbjct: 223 KIIKGTVATGFSTNSDGEVTEVKLEDGRTLEANIVVAGVGARPATSLFKGQLEEEKGGIK 282

Query: 241 TDGFFKTSIPGVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAQEEGKSTDV 300
           TDGFFKTS+P VYA+GDVAT+P+K+Y   RRVEH D+ARKSA QAVKAIKA EEGK+   
Sbjct: 283 TDGFFKTSVPDVYALGDVATFPMKMYGGTRRVEHADNARKSAAQAVKAIKAGEEGKTIPD 342

Query: 301 YDYLPYFYSRAFNLSWQFYGDNVGETILFGDNNPESSQPKFGTYWVKDGKVVGVFLEGGS 360
           YDYLPYFYSR F LSW+FYG+NVGE++LFGDN+P+S +PKFGTYWVKDGKVVGVFLEGG+
Sbjct: 343 YDYLPYFYSRFFKLSWEFYGENVGESVLFGDNDPKSPKPKFGTYWVKDGKVVGVFLEGGT 402

Query: 361 ADEYKAIAKLARVQPSVQSLDQLAKEGLSFASK 393
            +E+KAIAK+AR QPSV+SLD L++EGLSFA+K
Sbjct: 403 QEEHKAIAKVARAQPSVESLDVLSEEGLSFATK 435

BLAST of MS019549 vs. TAIR 10
Match: AT3G09940.2 (monodehydroascorbate reductase )

HSP 1 Score: 525.4 bits (1352), Expect = 3.9e-149
Identity = 259/393 (65.90%), Postives = 318/393 (80.92%), Query Frame = 0

Query: 1   VAPYERPALSKGYLFPESPARLPGFHVCVGSGGEKLLPEWYKEKGIELILSTEIVKADLA 60
           V P+ERP L+K Y+  E    L   +VC G+G  K  P WYKEK        EIVKADLA
Sbjct: 43  VPPFERPELTKVYIDLEVNPTLANIYVCAGTGEAKQYPNWYKEK--------EIVKADLA 102

Query: 61  AKALTSASGQTFKYQILIIATGSTVIRLSDFGVQGADAKNIFYLREVDDADKLVEAIKA- 120
           +K L S  G+ +KYQ L+IATGST IRLS+ GVQ AD KNIFYLRE++D+D+L  A++  
Sbjct: 103 SKTLVSDDGKIYKYQTLLIATGSTNIRLSEIGVQEADVKNIFYLREIEDSDELALAMELY 162

Query: 121 KKSGKAVIVGGGYIGLELGAALQMNNLDVTMVYPEPWCMPRLFTSGIAAFYEGYYKNKGI 180
            + GKAVI+GGG++GLE+ +AL+ NN +VTMV+PEPW + R FT+ IA+FYE YY NKGI
Sbjct: 163 VQRGKAVIIGGGFLGLEISSALRANNHEVTMVFPEPWLVHRFFTAEIASFYESYYANKGI 222

Query: 181 NIIKGTVAVGFTSDSNGEVKQVKLKDGRVLDADIVVVGVGGRPLISLFKGQVEEEKGGIK 240
            IIKGTVA GF+++S+GEV +VKL+DGR L+A+IVV GVG RP  SLFKGQ+EEEKGGIK
Sbjct: 223 KIIKGTVATGFSTNSDGEVTEVKLEDGRTLEANIVVAGVGARPATSLFKGQLEEEKGGIK 282

Query: 241 TDGFFKTSIPGVYAVGDVATYPLKLYNELRRVEHVDHARKSAEQAVKAIKAQEEGKSTDV 300
           TDGFFKTS+P VYA+GDVAT+P+K+Y   RRVEH D+ARKSA QAVKAIKA EEGK+   
Sbjct: 283 TDGFFKTSVPDVYALGDVATFPMKMYGGTRRVEHADNARKSAAQAVKAIKAGEEGKTIPD 342

Query: 301 YDYLPYFYSRAFNLSWQFYGDNVGETILFGDNNPESSQPKFGTYWVKDGKVVGVFLEGGS 360
           YDYLPYFYSR F LSW+FYG+NVGE++LFGDN+P+S +PKFGTYWVKDGKVVGVFLEGG+
Sbjct: 343 YDYLPYFYSRFFKLSWEFYGENVGESVLFGDNDPKSPKPKFGTYWVKDGKVVGVFLEGGT 402

Query: 361 ADEYKAIAKLARVQPSVQSLDQLAKEGLSFASK 393
            +E+KAIAK+AR QPSV+SLD L++EGLSFA+K
Sbjct: 403 QEEHKAIAKVARAQPSVESLDVLSEEGLSFATK 427

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022144639.13.9e-223100.00monodehydroascorbate reductase, seedling isozyme-like [Momordica charantia][more]
XP_022934571.13.4e-21193.64monodehydroascorbate reductase, seedling isozyme-like [Cucurbita moschata] >KAG6... [more]
XP_022982953.19.9e-21193.38monodehydroascorbate reductase, seedling isozyme-like [Cucurbita maxima][more]
XP_023526834.19.9e-21193.38monodehydroascorbate reductase, seedling isozyme-like [Cucurbita pepo subsp. pep... [more]
XP_038904751.14.6e-20892.37monodehydroascorbate reductase, seedling isozyme-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q427117.6e-19081.93Monodehydroascorbate reductase, seedling isozyme OS=Cucumis sativus OX=3659 PE=2... [more]
Q434973.5e-18779.90Monodehydroascorbate reductase OS=Solanum lycopersicum OX=4081 GN=AFRR PE=1 SV=1[more]
Q409776.0e-18780.41Monodehydroascorbate reductase OS=Pisum sativum OX=3888 PE=1 SV=1[more]
Q652L64.1e-18378.12Monodehydroascorbate reductase 3, cytosolic OS=Oryza sativa subsp. japonica OX=3... [more]
Q9LFA31.7e-18176.59Monodehydroascorbate reductase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1CSV71.9e-223100.00monodehydroascorbate reductase, seedling isozyme-like OS=Momordica charantia OX=... [more]
A0A6J1F2651.6e-21193.64monodehydroascorbate reductase, seedling isozyme-like OS=Cucurbita moschata OX=3... [more]
A0A6J1J6E34.8e-21193.38monodehydroascorbate reductase, seedling isozyme-like OS=Cucurbita maxima OX=366... [more]
A0A0A0LGL91.1e-20488.80Pyr_redox_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G775240... [more]
A0A5D3DNM46.3e-20388.80Monodehydroascorbate reductase, seedling isozyme-like OS=Cucumis melo var. makuw... [more]
Match NameE-valueIdentityDescription
AT3G52880.11.2e-18276.59monodehydroascorbate reductase 1 [more]
AT3G52880.21.2e-18276.59monodehydroascorbate reductase 1 [more]
AT5G03630.11.0e-18177.35Pyridine nucleotide-disulphide oxidoreductase family protein [more]
AT3G09940.12.4e-15467.18monodehydroascorbate reductase [more]
AT3G09940.23.9e-14965.90monodehydroascorbate reductase [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00368FADPNRcoord: 236..258
score: 42.46
coord: 74..92
score: 32.2
coord: 211..227
score: 29.53
coord: 124..142
score: 40.11
NoneNo IPR availablePRINTSPR00469PNDRDTASEIIcoord: 120..144
score: 24.59
coord: 246..264
score: 41.34
coord: 213..234
score: 26.27
coord: 75..83
score: 45.53
NoneNo IPR availablePANTHERPTHR43557:SF11MONODEHYDROASCORBATE REDUCTASE-LIKE ISOFORM X1coord: 1..393
NoneNo IPR availablePANTHERPTHR43557APOPTOSIS-INDUCING FACTOR 1coord: 1..393
IPR023753FAD/NAD(P)-binding domainPFAMPF07992Pyr_redox_2coord: 3..281
e-value: 9.1E-48
score: 163.0
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 88..219
e-value: 7.1E-99
score: 332.7
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 2..284
e-value: 7.1E-99
score: 332.7
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 35..289
IPR016156FAD/NAD-linked reductase, dimerisation domain superfamilyGENE3D3.30.390.30coord: 305..393
e-value: 7.7E-39
score: 133.7
IPR016156FAD/NAD-linked reductase, dimerisation domain superfamilySUPERFAMILY55424FAD/NAD-linked reductases, dimerisation (C-terminal) domaincoord: 300..391

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS019549.1MS019549.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016491 oxidoreductase activity