MS019525 (gene) Bitter gourd (TR) v1

Overview


Name	MS019525
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Carbonic anhydrase
Location	scaffold729: 188777 .. 191706 (-)
RNA-Seq Expression	MS019525
Synteny	MS019525

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCCTCCATTCTCCTCTATTTTTGTTTTCTCTCTTTTGTTTATTGGTGTTTTCGTGCAAGCCGACCGTAAGCCCTTTCCCTAACTTCTCCAATTTTCCCATTTCATTTTTCTCTTCTACTTTCCGGGAAAAAAAACACCATGGGTTTGTTTGTTTGTTTGTTTGTTTTCTGGGAAAATGAAAATGGCTGCAGAAGAGGAAGGAAATGAAGTACTCCTAGCTAGCTAATCCTTTTATTCAAGAAATGATTCTCTTCTAAACTGTGTGTTTTTCCTATTATTTTTGGGTGGACAGAGGTGGGGCAATCCGCTGCATTTACTTATGATGGCTCGCATGGACCTCAATACTGGGGAGAGTTGAGCCCAAGTTTTGCAGCATGCAAAAATGGAAAATCACAGTCACCAATAGACATTGTGAAGGACAAGACCTCGTTTGGCAAGCATCTTCAAACACTAATCAGAAAATATACTTCTGCTAATGCCACACTTGTCAACAACGGCTTTAACATTGGGGTTTGCCCTTCAAACCTATAACTTTTACCTCTCTTTTATTCCCCCATGCCCACATCATCAACATGCATACATACCTACATATGCATATGCATGCTTATCTATTTGCTAAGGTCTCATTTGCCAACTATTTTCATAAGTTGGATAACTATTTTAATTTTGTTTTTCTGTTTTTAAAAGTTATATATACTTGATTTCTCACAATTTATTCACATGTTTTAGAAACACATTTGAATTCTTTGTCAAATTTTAAAAACAGAAGTAGGTTTTTAGGTCTTTCAAATTTTGGCTTGAGTTTTAAAAACGTTTTAGAAAGTAGATAACAAAGAAACACAAGGTGGAAGTAGTGTTTTTAGACTTAATTTTTAAAAGTCAAATGGTTATCAATTGGGACCTTCGTTTTTAGTTTTATGTTTTTAAAAATTATGCTTTATTTTATACAATTTCATGAGTAAGTTTTTTGTATCTCTTAAAAACATATTTGAATTCTTAGCCAAATTTCAAAAACAAAAACAAGTTTTTGAAAAAAAAAAATATATATATATATATANNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGATTTTTAAATTTTTTATAAAAATTATATAACAAAATAAAGGAACGTATAAGGGAAAATAATGTTTCTAAACTTACTTTTCAAAAATGAAAAACTAAGAACAAACTTCCTTATTTCCTATTTTTTGTTTCTAGCTTGTGAAAAATCAACAGTTTCACATTGGCCTTTTTTATCCTTCTAATATCTTATTGCTTGTTCGTGTAGAAATACTTTTAAAATTATATACAATTTTATGTAAAAAGGGCTAATATTTTGGTCTTAATTTTTAAAAACATCAATATATCTTTTATCTTGTTCTTTCCCCTTCCTAAATTACATATCAGACCACCAAAATGAAAATGAATGTTCTATTCTTAAAAATTTGATTTTCCAGTGAAAATCTCTCTTAATTTAAAATTCTAAAGAATAAGTCAAAAACAATTTGTTTAGGTAAAACATAGAAGAACAAACCAATAAAAGGAATAATAAAAAGTTATGAGACACATTTATATTTCATTTTTCAAAAAGAGAGAGAGAGAGCCAATTGGCCAAACCTACGGATAATTTCTAATTCTTTTTCCGCCTTCAGATGCAATACTGTTCTATTAATACTCTGATAGGTTCATACATATTTATGCATTATATTTCAGAAAATGTGATAAATCAATAAATAAAAATGTGAGCCATATTGACATTTTTCTTTTCTTCCAAAAAATCAGTTACATTTCGGGGAGAATTCAGGTGGAGTAGCCGTAATAGATGGTAAAAACTACAGCCTCAAACAAATGCATTGGCATTCTCCTTCCGAGCACCGCCTCAACGGCGAGCAGTATGTTCACCTTCACCCCATCTCTCTCTCTCTGGGCCGGCCCATTATAGGCCCAAGCCCATCTATTATCAAGCTGACATGAAGCTTTTTTCCTAAATATTTTATTTCAAACTTATTATACATTCGAAAATTAATTTTACAAAAACATTAATACTTTGAAATTTGAAATATGTTCCAAGAATATGAAATCTAAAGTGAAAAGAAGATTTCAGAAAATTTTTAAGTTCTCTTAAACAATCGATTTGTATGAAAATCATGAAACTCATCAATCTCGACAGTTCATCTAAATCCAAACGGCCTAAAAAAGAACTGATTCTAGTTTTATCCTCGAATCAATCTAATCGGATCAAGTTCATCTGTAATACTAATTTCAAGGAGTTCTGGATTTGAAAATTACCTCCGTACTTTCCGATTGGAACAGATTCGCAGCCGAGCTTCATCTACTCCATCAGGCAGACGACGGCAGCCTCTCCGTCATAGCAATCCTCCTCCAATACGGCGATCCAGATCTCCTACTCCAGAAGGTACATCAATCAATCAGTCAATTTCACTCTCTCAAAACGAATCGAAAATTTTCGGAAACATACATTTTACAAATTACAATCACCGCAATCCGGCAAAATTGCAGATCCAAGACAAATTGGGGGAGTTAGCCAAGGAGCGATGTGGCGCCGATGAAGAATCTCACATTACAATCGAAAACCTAGACACGAGGCAATTGAGGAAGAAGACTCGCAAATATTACAGATACGTCGGCTCTCTCACCACTCCTCCCTGCTCCGAGAACGTCATCTGGAGCATTCTCGGCAAGGTAAATCAAATCTCCATTTATCAAGCTCCTCACGGCGGAACGGATTACCTTAGGGCTTAAGCATATAACATTCAAAATTACCTCGCAGGTGAGGACTATTTCGAAGGAGCAAGTTGAAGCACTGAAGGCGCCATTGGATTCGGCGTACAAAAACAACGCCCGGCCGGCGCAGGCGCTGAATGGCCGGAAGATTGAAATCTACGACGAACTCAGCGAGTAC

mRNA sequence

ATGGCTCCTCCATTCTCCTCTATTTTTGTTTTCTCTCTTTTGTTTATTGGTGTTTTCGTGCAAGCCGACCAGGTGGGGCAATCCGCTGCATTTACTTATGATGGCTCGCATGGACCTCAATACTGGGGAGAGTTGAGCCCAAGTTTTGCAGCATGCAAAAATGGAAAATCACAGTCACCAATAGACATTGTGAAGGACAAGACCTCGTTTGGCAAGCATCTTCAAACACTAATCAGAAAATATACTTCTGCTAATGCCACACTTGTCAACAACGGCTTTAACATTGGGTTACATTTCGGGGAGAATTCAGGTGGAGTAGCCGTAATAGATGGTAAAAACTACAGCCTCAAACAAATGCATTGGCATTCTCCTTCCGAGCACCGCCTCAACGGCGAGCAATTCGCAGCCGAGCTTCATCTACTCCATCAGGCAGACGACGGCAGCCTCTCCGTCATAGCAATCCTCCTCCAATACGGCGATCCAGATCTCCTACTCCAGAAGATCCAAGACAAATTGGGGGAGTTAGCCAAGGAGCGATGTGGCGCCGATGAAGAATCTCACATTACAATCGAAAACCTAGACACGAGGCAATTGAGGAAGAAGACTCGCAAATATTACAGATACGTCGGCTCTCTCACCACTCCTCCCTGCTCCGAGAACGTCATCTGGAGCATTCTCGGCAAGGTGAGGACTATTTCGAAGGAGCAAGTTGAAGCACTGAAGGCGCCATTGGATTCGGCGTACAAAAACAACGCCCGGCCGGCGCAGGCGCTGAATGGCCGGAAGATTGAAATCTACGACGAACTCAGCGAGTAC

Coding sequence (CDS)

ATGGCTCCTCCATTCTCCTCTATTTTTGTTTTCTCTCTTTTGTTTATTGGTGTTTTCGTGCAAGCCGACCAGGTGGGGCAATCCGCTGCATTTACTTATGATGGCTCGCATGGACCTCAATACTGGGGAGAGTTGAGCCCAAGTTTTGCAGCATGCAAAAATGGAAAATCACAGTCACCAATAGACATTGTGAAGGACAAGACCTCGTTTGGCAAGCATCTTCAAACACTAATCAGAAAATATACTTCTGCTAATGCCACACTTGTCAACAACGGCTTTAACATTGGGTTACATTTCGGGGAGAATTCAGGTGGAGTAGCCGTAATAGATGGTAAAAACTACAGCCTCAAACAAATGCATTGGCATTCTCCTTCCGAGCACCGCCTCAACGGCGAGCAATTCGCAGCCGAGCTTCATCTACTCCATCAGGCAGACGACGGCAGCCTCTCCGTCATAGCAATCCTCCTCCAATACGGCGATCCAGATCTCCTACTCCAGAAGATCCAAGACAAATTGGGGGAGTTAGCCAAGGAGCGATGTGGCGCCGATGAAGAATCTCACATTACAATCGAAAACCTAGACACGAGGCAATTGAGGAAGAAGACTCGCAAATATTACAGATACGTCGGCTCTCTCACCACTCCTCCCTGCTCCGAGAACGTCATCTGGAGCATTCTCGGCAAGGTGAGGACTATTTCGAAGGAGCAAGTTGAAGCACTGAAGGCGCCATTGGATTCGGCGTACAAAAACAACGCCCGGCCGGCGCAGGCGCTGAATGGCCGGAAGATTGAAATCTACGACGAACTCAGCGAGTAC

Protein sequence

MAPPFSSIFVFSLLFIGVFVQADQVGQSAAFTYDGSHGPQYWGELSPSFAACKNGKSQSPIDIVKDKTSFGKHLQTLIRKYTSANATLVNNGFNIGLHFGENSGGVAVIDGKNYSLKQMHWHSPSEHRLNGEQFAAELHLLHQADDGSLSVIAILLQYGDPDLLLQKIQDKLGELAKERCGADEESHITIENLDTRQLRKKTRKYYRYVGSLTTPPCSENVIWSILGKVRTISKEQVEALKAPLDSAYKNNARPAQALNGRKIEIYDELSEY

Homology

[Close]

BLAST of MS019525 vs. NCBI nr
Match: XP_022144656.1 (alpha carbonic anhydrase 1, chloroplastic [Momordica charantia])

HSP 1 Score: 547.7 bits (1410), Expect = 5.5e-152
Identity = 272/272 (100.00%), Postives = 272/272 (100.00%), Query Frame = 0


Query: 1   MAPPFSSIFVFSLLFIGVFVQADQVGQSAAFTYDGSHGPQYWGELSPSFAACKNGKSQSP 60
           MAPPFSSIFVFSLLFIGVFVQADQVGQSAAFTYDGSHGPQYWGELSPSFAACKNGKSQSP
Sbjct: 1   MAPPFSSIFVFSLLFIGVFVQADQVGQSAAFTYDGSHGPQYWGELSPSFAACKNGKSQSP 60

Query: 61  IDIVKDKTSFGKHLQTLIRKYTSANATLVNNGFNIGLHFGENSGGVAVIDGKNYSLKQMH 120
           IDIVKDKTSFGKHLQTLIRKYTSANATLVNNGFNIGLHFGENSGGVAVIDGKNYSLKQMH
Sbjct: 61  IDIVKDKTSFGKHLQTLIRKYTSANATLVNNGFNIGLHFGENSGGVAVIDGKNYSLKQMH 120

Query: 121 WHSPSEHRLNGEQFAAELHLLHQADDGSLSVIAILLQYGDPDLLLQKIQDKLGELAKERC 180
           WHSPSEHRLNGEQFAAELHLLHQADDGSLSVIAILLQYGDPDLLLQKIQDKLGELAKERC
Sbjct: 121 WHSPSEHRLNGEQFAAELHLLHQADDGSLSVIAILLQYGDPDLLLQKIQDKLGELAKERC 180

Query: 181 GADEESHITIENLDTRQLRKKTRKYYRYVGSLTTPPCSENVIWSILGKVRTISKEQVEAL 240
           GADEESHITIENLDTRQLRKKTRKYYRYVGSLTTPPCSENVIWSILGKVRTISKEQVEAL
Sbjct: 181 GADEESHITIENLDTRQLRKKTRKYYRYVGSLTTPPCSENVIWSILGKVRTISKEQVEAL 240

Query: 241 KAPLDSAYKNNARPAQALNGRKIEIYDELSEY 273
           KAPLDSAYKNNARPAQALNGRKIEIYDELSEY
Sbjct: 241 KAPLDSAYKNNARPAQALNGRKIEIYDELSEY 272

[Close]

BLAST of MS019525 vs. NCBI nr
Match: XP_004149223.1 (alpha carbonic anhydrase 1, chloroplastic isoform X2 [Cucumis sativus])

HSP 1 Score: 435.6 bits (1119), Expect = 3.0e-118
Identity = 208/272 (76.47%), Postives = 238/272 (87.50%), Query Frame = 0


Query: 1   MAPPFSSIFVFSLLFIGVFVQADQVGQSAAFTYDGSHGPQYWGELSPSFAACKNGKSQSP 60
           MAPPFSSIFV SLLF+G FVQA    QSAAFTY+GSHGPQ WG LSPS+AAC NGK QSP
Sbjct: 1   MAPPFSSIFVVSLLFLGFFVQAHDGVQSAAFTYEGSHGPQKWGSLSPSYAACSNGKLQSP 60

Query: 61  IDIVKDKTSFGKHLQTLIRKYTSANATLVNNGFNIGLHFGENSGGVAVIDGKNYSLKQMH 120
           +DI KD + FGK LQTL RKY+ ANATL NNGFNIG+HF ENSGG A+IDGKNY L+Q+H
Sbjct: 61  VDISKDHSVFGKELQTLARKYSIANATLTNNGFNIGVHFWENSGGTAIIDGKNYILRQLH 120

Query: 121 WHSPSEHRLNGEQFAAELHLLHQADDGSLSVIAILLQYGDPDLLLQKIQDKLGELAKERC 180
           WHSP+EHRLNGEQ+AAELHL+HQA+DG+LSVIAILLQ G+P+ LL KIQDKL ELA E+C
Sbjct: 121 WHSPAEHRLNGEQYAAELHLIHQAEDGTLSVIAILLQIGEPEPLLAKIQDKLVELANEKC 180

Query: 181 GADEESHITIENLDTRQLRKKTRKYYRYVGSLTTPPCSENVIWSILGKVRTISKEQVEAL 240
           G DE +H+ + +LDT+ LRKKTRKYYRY+GSLTTPPC+ENVIW++LGKVRTIS++Q+EAL
Sbjct: 181 GGDEVAHVALGDLDTKHLRKKTRKYYRYIGSLTTPPCTENVIWNVLGKVRTISQQQLEAL 240

Query: 241 KAPLDSAYKNNARPAQALNGRKIEIYDELSEY 273
           KAPLD  YKNNARP Q LNGRKIEIYDELSEY
Sbjct: 241 KAPLDPVYKNNARPVQPLNGRKIEIYDELSEY 272

[Close]

BLAST of MS019525 vs. NCBI nr
Match: XP_038903044.1 (alpha carbonic anhydrase 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 434.9 bits (1117), Expect = 5.2e-118
Identity = 209/273 (76.56%), Postives = 239/273 (87.55%), Query Frame = 0


Query: 1   MAPPFSSIFVFSLLFIGVFVQA-DQVGQSAAFTYDGSHGPQYWGELSPSFAACKNGKSQS 60
           MAPPFSSIFVFSLLFIG FVQA +Q GQSA FTY+GSHGP+ WG LSPS+A C NGKSQS
Sbjct: 1   MAPPFSSIFVFSLLFIGAFVQAYEQAGQSAEFTYEGSHGPENWGSLSPSYATCSNGKSQS 60

Query: 61  PIDIVKDKTSFGKHLQTLIRKYTSANATLVNNGFNIGLHFGENSGGVAVIDGKNYSLKQM 120
           PI+IVKD    GKHLQTL+R Y  ANATL+NNGFNIG+HFGENSGGVAVIDGKNYSL+Q+
Sbjct: 61  PINIVKDNAVIGKHLQTLVRGYHVANATLINNGFNIGMHFGENSGGVAVIDGKNYSLQQL 120

Query: 121 HWHSPSEHRLNGEQFAAELHLLHQADDGSLSVIAILLQYGDPDLLLQKIQDKLGELAKER 180
           HWHSPSEHRL+GEQFAAELHLLH+ADDGSL+V+ ILLQ G+ D LL  I DKL ELA E 
Sbjct: 121 HWHSPSEHRLDGEQFAAELHLLHRADDGSLAVVGILLQIGEADPLLADIHDKLVELANES 180

Query: 181 CGADEESHITIENLDTRQLRKKTRKYYRYVGSLTTPPCSENVIWSILGKVRTISKEQVEA 240
           CG++EE+HI +  LD + LRKKTRKY+RY+GSLTTPPC+ENVIWS+LGKVRTIS++Q+E+
Sbjct: 181 CGSNEEAHIAVGGLDIKHLRKKTRKYFRYIGSLTTPPCTENVIWSVLGKVRTISQQQIES 240

Query: 241 LKAPLDSAYKNNARPAQALNGRKIEIYDELSEY 273
           LKAPL+  YKNNARP Q LNGRKIEIYDELSE+
Sbjct: 241 LKAPLNPVYKNNARPVQPLNGRKIEIYDELSEH 273

[Close]

BLAST of MS019525 vs. NCBI nr
Match: XP_011652015.1 (alpha carbonic anhydrase 1, chloroplastic isoform X1 [Cucumis sativus] >KGN59137.1 hypothetical protein Csa_002208 [Cucumis sativus])

HSP 1 Score: 433.7 bits (1114), Expect = 1.2e-117
Identity = 209/273 (76.56%), Postives = 239/273 (87.55%), Query Frame = 0


Query: 1   MAPPFSSIFVFSLLFIGVFVQADQVG-QSAAFTYDGSHGPQYWGELSPSFAACKNGKSQS 60
           MAPPFSSIFV SLLF+G FVQA   G QSAAFTY+GSHGPQ WG LSPS+AAC NGK QS
Sbjct: 1   MAPPFSSIFVVSLLFLGFFVQAHGHGVQSAAFTYEGSHGPQKWGSLSPSYAACSNGKLQS 60

Query: 61  PIDIVKDKTSFGKHLQTLIRKYTSANATLVNNGFNIGLHFGENSGGVAVIDGKNYSLKQM 120
           P+DI KD + FGK LQTL RKY+ ANATL NNGFNIG+HF ENSGG A+IDGKNY L+Q+
Sbjct: 61  PVDISKDHSVFGKELQTLARKYSIANATLTNNGFNIGVHFWENSGGTAIIDGKNYILRQL 120

Query: 121 HWHSPSEHRLNGEQFAAELHLLHQADDGSLSVIAILLQYGDPDLLLQKIQDKLGELAKER 180
           HWHSP+EHRLNGEQ+AAELHL+HQA+DG+LSVIAILLQ G+P+ LL KIQDKL ELA E+
Sbjct: 121 HWHSPAEHRLNGEQYAAELHLIHQAEDGTLSVIAILLQIGEPEPLLAKIQDKLVELANEK 180

Query: 181 CGADEESHITIENLDTRQLRKKTRKYYRYVGSLTTPPCSENVIWSILGKVRTISKEQVEA 240
           CG DE +H+ + +LDT+ LRKKTRKYYRY+GSLTTPPC+ENVIW++LGKVRTIS++Q+EA
Sbjct: 181 CGGDEVAHVALGDLDTKHLRKKTRKYYRYIGSLTTPPCTENVIWNVLGKVRTISQQQLEA 240

Query: 241 LKAPLDSAYKNNARPAQALNGRKIEIYDELSEY 273
           LKAPLD  YKNNARP Q LNGRKIEIYDELSEY
Sbjct: 241 LKAPLDPVYKNNARPVQPLNGRKIEIYDELSEY 273

[Close]

BLAST of MS019525 vs. NCBI nr
Match: XP_008442846.1 (PREDICTED: alpha carbonic anhydrase 1, chloroplastic [Cucumis melo] >KAA0043878.1 alpha carbonic anhydrase 1 [Cucumis melo var. makuwa] >TYK25259.1 alpha carbonic anhydrase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 428.3 bits (1100), Expect = 4.9e-116
Identity = 204/273 (74.73%), Postives = 240/273 (87.91%), Query Frame = 0


Query: 1   MAPPFSSIFVFSLLFIGVFVQA-DQVGQSAAFTYDGSHGPQYWGELSPSFAACKNGKSQS 60
           MAPPFSSIF FSLLF+GV VQA DQVGQSA FTY+GSHGP+ WG LSPS+A C NGK QS
Sbjct: 1   MAPPFSSIFAFSLLFVGVLVQAHDQVGQSAVFTYEGSHGPEKWGSLSPSYATCSNGKFQS 60

Query: 61  PIDIVKDKTSFGKHLQTLIRKYTSANATLVNNGFNIGLHFGENSGGVAVIDGKNYSLKQM 120
           P+DI KD + FGK LQTL+RKY  ANATL NNG NIG+HF +NSGGVA+IDGKNY+LKQ+
Sbjct: 61  PVDISKDNSVFGKKLQTLVRKYNIANATLTNNGVNIGVHFWKNSGGVAIIDGKNYTLKQL 120

Query: 121 HWHSPSEHRLNGEQFAAELHLLHQADDGSLSVIAILLQYGDPDLLLQKIQDKLGELAKER 180
           HWHSP+EHRLNG+Q+AAELHL+HQADDG+ SVIAILLQ GDP+ LL KI DKL ELA E+
Sbjct: 121 HWHSPAEHRLNGKQYAAELHLVHQADDGTYSVIAILLQIGDPEPLLAKIHDKLVELANEK 180

Query: 181 CGADEESHITIENLDTRQLRKKTRKYYRYVGSLTTPPCSENVIWSILGKVRTISKEQVEA 240
           CG++EE+HI + +LDT+ LRKKTRKY+RY+GSLTTPPC+ENVIW++LGKVRTIS++QV+A
Sbjct: 181 CGSNEEAHIALGDLDTKHLRKKTRKYFRYIGSLTTPPCTENVIWNVLGKVRTISQQQVDA 240

Query: 241 LKAPLDSAYKNNARPAQALNGRKIEIYDELSEY 273
           LKAPL+  YKNNARP Q L GRKIEIYDELS++
Sbjct: 241 LKAPLNPVYKNNARPVQPLYGRKIEIYDELSKH 273

[Close]

BLAST of MS019525 vs. ExPASy Swiss-Prot
Match: O04846 (Alpha carbonic anhydrase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ACA1 PE=1 SV=2)

HSP 1 Score: 270.0 bits (689), Expect = 2.9e-71
Identity = 128/258 (49.61%), Postives = 183/258 (70.93%), Query Frame = 0


Query: 11  FSLLFIGVFVQADQVGQSAAFTYDGSHGPQYWGELSPSFAACKNGKSQSPIDIVKDKTSF 70
           FS+  I +   AD   +   F Y G +GP  WG L+P F  C  GK QSPIDI + +  +
Sbjct: 12  FSMSLICI-APADAQTEGVVFGYKGKNGPNQWGHLNPHFTTCAVGKLQSPIDIQRRQIFY 71

Query: 71  GKHLQTLIRKYTSANATLVNNGFNIGLHFGENSGGVAVIDGKNYSLKQMHWHSPSEHRLN 130
              L ++ R+Y   NATLVN+  N+ + FGE +G V +I+ KNY+L QMHWH+PSEH L+
Sbjct: 72  NHKLNSIHREYYFTNATLVNHVCNVAMFFGEGAGDV-IIENKNYTLLQMHWHTPSEHHLH 131

Query: 131 GEQFAAELHLLHQADDGSLSVIAILLQYGDPDLLLQKIQDKLGELAKERCGADEESHITI 190
           G Q+AAELH++HQA DGS +V+A L + G  +  L ++++KL +L +ER   +  + + +
Sbjct: 132 GVQYAAELHMVHQAKDGSFAVVASLFKIGTEEPFLSQMKEKLVKLKEERLKGNHTAQVEV 191

Query: 191 ENLDTRQLRKKTRKYYRYVGSLTTPPCSENVIWSILGKVRTISKEQVEALKAPLDSAYKN 250
             +DTR + +KTRKYYRY+GSLTTPPCSENV W+ILGKVR++SKEQVE L++PLD+++KN
Sbjct: 192 GRIDTRHIERKTRKYYRYIGSLTTPPCSENVSWTILGKVRSMSKEQVELLRSPLDTSFKN 251

Query: 251 NARPAQALNGRKIEIYDE 269
           N+RP Q LNGR++E++ +
Sbjct: 252 NSRPCQPLNGRRVEMFHD 267

[Close]

BLAST of MS019525 vs. ExPASy Swiss-Prot
Match: Q8L817 (Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana OX=3702 GN=ACA7 PE=2 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 2.4e-49
Identity = 96/245 (39.18%), Postives = 151/245 (61.63%), Query Frame = 0


Query: 24  QVGQSAAFTY--DGSHGPQYWGELSPSFAACKNGKSQSPIDIVKDKTSFGKHLQTLIRKY 83
           +V     F Y  +   GP+ WGEL P +  C  G+ QSPID++ ++ +   HL  L R Y
Sbjct: 33  EVEDEREFNYKKNDEKGPERWGELKPEWEMCGKGEMQSPIDLMNERVNIVSHLGRLNRDY 92

Query: 84  TSANATLVNNGFNIGLHFGENSGGVAVIDGKNYSLKQMHWHSPSEHRLNGEQFAAELHLL 143
             +NATL N G +I L F E+  G   I+G  Y L+Q+HWHSPSEH +NG +FA ELH++
Sbjct: 93  NPSNATLKNRGHDIMLKF-EDGAGTIKINGFEYELQQLHWHSPSEHTINGRRFALELHMV 152

Query: 144 HQADDGSLSVIAILLQYGDPDLLLQKIQDKLGELAKERCGADEESHITIENLDTRQLRKK 203
           H+  +  ++V+ +L + G  D  ++ ++ +L  +A+      EE+   +  +D  +++  
Sbjct: 153 HEGRNRRMAVVTVLYKIGRADTFIRSLEKELEGIAEM-----EEAEKNVGMIDPTKIKIG 212

Query: 204 TRKYYRYVGSLTTPPCSENVIWSILGKVRTISKEQVEALKAPLDSAYKNNARPAQALNGR 263
           +RKYYRY GSLTTPPC++NV WS++ KVRT++++QV+ L+  +     +NARP Q  N R
Sbjct: 213 SRKYYRYTGSLTTPPCTQNVTWSVVRKVRTVTRKQVKLLRVAVHDDANSNARPVQPTNKR 271

Query: 264 KIEIY 267
            + +Y
Sbjct: 273 IVHLY 271

[Close]

BLAST of MS019525 vs. ExPASy Swiss-Prot
Match: F4HUC4 (Alpha carbonic anhydrase 5 OS=Arabidopsis thaliana OX=3702 GN=ACA5 PE=3 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 3.2e-46
Identity = 101/266 (37.97%), Postives = 152/266 (57.14%), Query Frame = 0


Query: 9   FVFSLLFIGVFVQAD-----QVGQSAAFTYD--GSHGPQYWGELSPSFAACKNGKSQSPI 68
           +VF L+FI + + +      +V     F Y+  G  GP+ WG L P +A C  G  QSPI
Sbjct: 8   YVFFLIFISITIVSSSPDHGEVEDETQFNYEKKGEKGPENWGRLKPEWAMCGKGNMQSPI 67

Query: 69  DIVKDKTSFGKHLQTLIRKYTSANATLVNNGFNIGLHF-GENSGGVAVIDGKNYSLKQMH 128
           D+   +     +L  L  +Y  +NAT+ N G +I + F G N+G    I+G  Y L+Q+H
Sbjct: 68  DLTDKRVLIDHNLGYLRSQYLPSNATIKNRGHDIMMKFEGGNAGLGITINGTEYKLQQIH 127

Query: 129 WHSPSEHRLNGEQFAAELHLLHQADDGSLSVIAILLQYGDPDLLLQKIQDKLGELAKERC 188
           WHSPSEH LNG++F  E H++HQ+ DG  +V+A   + G PD  L  ++  L     +R 
Sbjct: 128 WHSPSEHTLNGKRFVLEEHMVHQSKDGRNAVVAFFYKLGKPDYFLLTLERYL-----KRI 187

Query: 189 GADEESHITIENLDTRQLRKKTRKYYRYVGSLTTPPCSENVIWSILGKVRTISKEQVEAL 248
               ES   +E +  R    +++ YYR++GSLTTPPCSENVIW+I  ++RT++ +Q+  L
Sbjct: 188 TDTHESQEFVEMVHPRTFGFESKHYYRFIGSLTTPPCSENVIWTISKEMRTVTLKQLIML 247

Query: 249 KAPLDSAYKNNARPAQALNGRKIEIY 267
           +  +     +NARP Q  N R + +Y
Sbjct: 248 RVTVHDQSNSNARPLQRKNERPVALY 268

[Close]

BLAST of MS019525 vs. ExPASy Swiss-Prot
Match: Q9FM99 (Alpha carbonic anhydrase 8 OS=Arabidopsis thaliana OX=3702 GN=ACA8 PE=3 SV=3)

HSP 1 Score: 183.0 bits (463), Expect = 4.7e-45
Identity = 98/246 (39.84%), Postives = 142/246 (57.72%), Query Frame = 0


Query: 24  QVGQSAAFTYD--GSHGPQYWGELSPSFAACKNGKSQSPIDIVKDKTSFGKHLQTLIRKY 83
           +V     F+Y+  G+ GP  WG L   +  C  GK QSPID+             L  +Y
Sbjct: 133 EVEDETEFSYETKGNKGPAKWGTLDAEWKMCGIGKMQSPIDLRDKNVVVSNKFGLLRSQY 192

Query: 84  TSANATLVNNGFNIGLHF-GENSGGVAVIDGKNYSLKQMHWHSPSEHRLNGEQFAAELHL 143
             +N T+ N G +I L F G N G    I G  Y L+Q+HWHSPSEH +NG++FA E HL
Sbjct: 193 LPSNTTIKNRGHDIMLKFKGGNKGIGVTIRGTRYQLQQLHWHSPSEHTINGKRFALEEHL 252

Query: 144 LHQADDGSLSVIAILLQYGDPDLLLQKIQDKLGELAKERCGADEESHITIENLDTRQLRK 203
           +H++ D   +V+A L   G  D  L  ++ +L ++         E H+ I  +D ++L  
Sbjct: 253 VHESKDKRYAVVAFLYNLGASDPFLFSLEKQLKKITDTHA---SEEHVGI--IDPKKLSF 312

Query: 204 KTRKYYRYVGSLTTPPCSENVIWSILGKVRTISKEQVEALKAPLDSAYKNNARPAQALNG 263
           +++ YYRY GSLT PPCSENVIWS+  ++RT+S +QV+ L+  +  A  +NARP QA+N 
Sbjct: 313 ESKHYYRYSGSLTAPPCSENVIWSVSKEIRTVSSKQVKLLRVAVHDASDSNARPLQAVNK 372

Query: 264 RKIEIY 267
           RK+ +Y
Sbjct: 373 RKVYLY 373

[Close]

BLAST of MS019525 vs. ExPASy Swiss-Prot
Match: F4IHR4 (Alpha carbonic anhydrase 2 OS=Arabidopsis thaliana OX=3702 GN=ACA2 PE=2 SV=2)

HSP 1 Score: 182.2 bits (461), Expect = 8.0e-45
Identity = 88/225 (39.11%), Postives = 137/225 (60.89%), Query Frame = 0


Query: 9   FVFSLLFIGVFVQADQVGQSAAFTYDGS--HGPQYWGELSPSFAACKNGKSQSPIDIVKD 68
           FV ++  +       +V     F+Y+ +  +GP  WG+L P +  C  G+ QSPID++  
Sbjct: 17  FVTTVSCLSAATDYREVEDEHEFSYEWNQENGPAKWGKLRPEWKMCGKGEMQSPIDLMNK 76

Query: 69  KTSFGKHLQTLIRKYTSANATLVNNGFNIGLHFGENSGGVAVIDGKNYSLKQMHWHSPSE 128
           +     HL+ L R Y   NATL N G ++ L FGE   G   ++G  Y L Q+HWHSPSE
Sbjct: 77  RVRLVTHLKKLTRHYKPCNATLKNRGHDMMLKFGEEGSGSITVNGTEYKLLQLHWHSPSE 136

Query: 129 HRLNGEQFAAELHLLHQADDGSLSVIAILLQYGDPDLLLQKIQDKLGELAKERCGADEES 188
           H +NG +FA ELH++H+  +GSL+V+ +L + G PD  L  +++KL  +  +      E+
Sbjct: 137 HTMNGRRFALELHMVHENINGSLAVVTVLYKIGRPDSFLGLLENKLSAITDQ-----NEA 196

Query: 189 HITIENLDTRQLRKKTRKYYRYVGSLTTPPCSENVIWSILGKVRT 232
              ++ +D R ++  +RK+YRY+GSLTTPPC++NVIW+++ KV T
Sbjct: 197 EKYVDVIDPRDIKIGSRKFYRYIGSLTTPPCTQNVIWTVVKKVNT 236

[Close]

BLAST of MS019525 vs. ExPASy TrEMBL
Match: A0A6J1CU20 (Carbonic anhydrase OS=Momordica charantia OX=3673 GN=LOC111014291 PE=3 SV=1)

HSP 1 Score: 547.7 bits (1410), Expect = 2.7e-152
Identity = 272/272 (100.00%), Postives = 272/272 (100.00%), Query Frame = 0


Query: 1   MAPPFSSIFVFSLLFIGVFVQADQVGQSAAFTYDGSHGPQYWGELSPSFAACKNGKSQSP 60
           MAPPFSSIFVFSLLFIGVFVQADQVGQSAAFTYDGSHGPQYWGELSPSFAACKNGKSQSP
Sbjct: 1   MAPPFSSIFVFSLLFIGVFVQADQVGQSAAFTYDGSHGPQYWGELSPSFAACKNGKSQSP 60

Query: 61  IDIVKDKTSFGKHLQTLIRKYTSANATLVNNGFNIGLHFGENSGGVAVIDGKNYSLKQMH 120
           IDIVKDKTSFGKHLQTLIRKYTSANATLVNNGFNIGLHFGENSGGVAVIDGKNYSLKQMH
Sbjct: 61  IDIVKDKTSFGKHLQTLIRKYTSANATLVNNGFNIGLHFGENSGGVAVIDGKNYSLKQMH 120

Query: 121 WHSPSEHRLNGEQFAAELHLLHQADDGSLSVIAILLQYGDPDLLLQKIQDKLGELAKERC 180
           WHSPSEHRLNGEQFAAELHLLHQADDGSLSVIAILLQYGDPDLLLQKIQDKLGELAKERC
Sbjct: 121 WHSPSEHRLNGEQFAAELHLLHQADDGSLSVIAILLQYGDPDLLLQKIQDKLGELAKERC 180

Query: 181 GADEESHITIENLDTRQLRKKTRKYYRYVGSLTTPPCSENVIWSILGKVRTISKEQVEAL 240
           GADEESHITIENLDTRQLRKKTRKYYRYVGSLTTPPCSENVIWSILGKVRTISKEQVEAL
Sbjct: 181 GADEESHITIENLDTRQLRKKTRKYYRYVGSLTTPPCSENVIWSILGKVRTISKEQVEAL 240

Query: 241 KAPLDSAYKNNARPAQALNGRKIEIYDELSEY 273
           KAPLDSAYKNNARPAQALNGRKIEIYDELSEY
Sbjct: 241 KAPLDSAYKNNARPAQALNGRKIEIYDELSEY 272

[Close]

BLAST of MS019525 vs. ExPASy TrEMBL
Match: A0A0A0LEL1 (Alpha-carbonic anhydrase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G776970 PE=3 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 5.6e-118
Identity = 209/273 (76.56%), Postives = 239/273 (87.55%), Query Frame = 0


Query: 1   MAPPFSSIFVFSLLFIGVFVQADQVG-QSAAFTYDGSHGPQYWGELSPSFAACKNGKSQS 60
           MAPPFSSIFV SLLF+G FVQA   G QSAAFTY+GSHGPQ WG LSPS+AAC NGK QS
Sbjct: 1   MAPPFSSIFVVSLLFLGFFVQAHGHGVQSAAFTYEGSHGPQKWGSLSPSYAACSNGKLQS 60

Query: 61  PIDIVKDKTSFGKHLQTLIRKYTSANATLVNNGFNIGLHFGENSGGVAVIDGKNYSLKQM 120
           P+DI KD + FGK LQTL RKY+ ANATL NNGFNIG+HF ENSGG A+IDGKNY L+Q+
Sbjct: 61  PVDISKDHSVFGKELQTLARKYSIANATLTNNGFNIGVHFWENSGGTAIIDGKNYILRQL 120

Query: 121 HWHSPSEHRLNGEQFAAELHLLHQADDGSLSVIAILLQYGDPDLLLQKIQDKLGELAKER 180
           HWHSP+EHRLNGEQ+AAELHL+HQA+DG+LSVIAILLQ G+P+ LL KIQDKL ELA E+
Sbjct: 121 HWHSPAEHRLNGEQYAAELHLIHQAEDGTLSVIAILLQIGEPEPLLAKIQDKLVELANEK 180

Query: 181 CGADEESHITIENLDTRQLRKKTRKYYRYVGSLTTPPCSENVIWSILGKVRTISKEQVEA 240
           CG DE +H+ + +LDT+ LRKKTRKYYRY+GSLTTPPC+ENVIW++LGKVRTIS++Q+EA
Sbjct: 181 CGGDEVAHVALGDLDTKHLRKKTRKYYRYIGSLTTPPCTENVIWNVLGKVRTISQQQLEA 240

Query: 241 LKAPLDSAYKNNARPAQALNGRKIEIYDELSEY 273
           LKAPLD  YKNNARP Q LNGRKIEIYDELSEY
Sbjct: 241 LKAPLDPVYKNNARPVQPLNGRKIEIYDELSEY 273

[Close]

BLAST of MS019525 vs. ExPASy TrEMBL
Match: A0A5A7TLZ2 (Alpha carbonic anhydrase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G003920 PE=3 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 2.4e-116
Identity = 204/273 (74.73%), Postives = 240/273 (87.91%), Query Frame = 0


Query: 1   MAPPFSSIFVFSLLFIGVFVQA-DQVGQSAAFTYDGSHGPQYWGELSPSFAACKNGKSQS 60
           MAPPFSSIF FSLLF+GV VQA DQVGQSA FTY+GSHGP+ WG LSPS+A C NGK QS
Sbjct: 1   MAPPFSSIFAFSLLFVGVLVQAHDQVGQSAVFTYEGSHGPEKWGSLSPSYATCSNGKFQS 60

Query: 61  PIDIVKDKTSFGKHLQTLIRKYTSANATLVNNGFNIGLHFGENSGGVAVIDGKNYSLKQM 120
           P+DI KD + FGK LQTL+RKY  ANATL NNG NIG+HF +NSGGVA+IDGKNY+LKQ+
Sbjct: 61  PVDISKDNSVFGKKLQTLVRKYNIANATLTNNGVNIGVHFWKNSGGVAIIDGKNYTLKQL 120

Query: 121 HWHSPSEHRLNGEQFAAELHLLHQADDGSLSVIAILLQYGDPDLLLQKIQDKLGELAKER 180
           HWHSP+EHRLNG+Q+AAELHL+HQADDG+ SVIAILLQ GDP+ LL KI DKL ELA E+
Sbjct: 121 HWHSPAEHRLNGKQYAAELHLVHQADDGTYSVIAILLQIGDPEPLLAKIHDKLVELANEK 180

Query: 181 CGADEESHITIENLDTRQLRKKTRKYYRYVGSLTTPPCSENVIWSILGKVRTISKEQVEA 240
           CG++EE+HI + +LDT+ LRKKTRKY+RY+GSLTTPPC+ENVIW++LGKVRTIS++QV+A
Sbjct: 181 CGSNEEAHIALGDLDTKHLRKKTRKYFRYIGSLTTPPCTENVIWNVLGKVRTISQQQVDA 240

Query: 241 LKAPLDSAYKNNARPAQALNGRKIEIYDELSEY 273
           LKAPL+  YKNNARP Q L GRKIEIYDELS++
Sbjct: 241 LKAPLNPVYKNNARPVQPLYGRKIEIYDELSKH 273

[Close]

BLAST of MS019525 vs. ExPASy TrEMBL
Match: A0A1S3B6Q0 (alpha carbonic anhydrase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103486616 PE=3 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 2.4e-116
Identity = 204/273 (74.73%), Postives = 240/273 (87.91%), Query Frame = 0


Query: 1   MAPPFSSIFVFSLLFIGVFVQA-DQVGQSAAFTYDGSHGPQYWGELSPSFAACKNGKSQS 60
           MAPPFSSIF FSLLF+GV VQA DQVGQSA FTY+GSHGP+ WG LSPS+A C NGK QS
Sbjct: 1   MAPPFSSIFAFSLLFVGVLVQAHDQVGQSAVFTYEGSHGPEKWGSLSPSYATCSNGKFQS 60

Query: 61  PIDIVKDKTSFGKHLQTLIRKYTSANATLVNNGFNIGLHFGENSGGVAVIDGKNYSLKQM 120
           P+DI KD + FGK LQTL+RKY  ANATL NNG NIG+HF +NSGGVA+IDGKNY+LKQ+
Sbjct: 61  PVDISKDNSVFGKKLQTLVRKYNIANATLTNNGVNIGVHFWKNSGGVAIIDGKNYTLKQL 120

Query: 121 HWHSPSEHRLNGEQFAAELHLLHQADDGSLSVIAILLQYGDPDLLLQKIQDKLGELAKER 180
           HWHSP+EHRLNG+Q+AAELHL+HQADDG+ SVIAILLQ GDP+ LL KI DKL ELA E+
Sbjct: 121 HWHSPAEHRLNGKQYAAELHLVHQADDGTYSVIAILLQIGDPEPLLAKIHDKLVELANEK 180

Query: 181 CGADEESHITIENLDTRQLRKKTRKYYRYVGSLTTPPCSENVIWSILGKVRTISKEQVEA 240
           CG++EE+HI + +LDT+ LRKKTRKY+RY+GSLTTPPC+ENVIW++LGKVRTIS++QV+A
Sbjct: 181 CGSNEEAHIALGDLDTKHLRKKTRKYFRYIGSLTTPPCTENVIWNVLGKVRTISQQQVDA 240

Query: 241 LKAPLDSAYKNNARPAQALNGRKIEIYDELSEY 273
           LKAPL+  YKNNARP Q L GRKIEIYDELS++
Sbjct: 241 LKAPLNPVYKNNARPVQPLYGRKIEIYDELSKH 273

[Close]

BLAST of MS019525 vs. ExPASy TrEMBL
Match: A0A6J1J129 (Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111481722 PE=3 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 1.3e-111
Identity = 202/273 (73.99%), Postives = 231/273 (84.62%), Query Frame = 0


Query: 1   MAPPFSSIFVFSLLFIGVFVQADQVG-QSAAFTYDGSHGPQYWGELSPSFAACKNGKSQS 60
           MAP  SSIFV SLL IGVFVQ      QSA F+Y+GS+GP++WG +S  FA C NGKSQS
Sbjct: 1   MAPSVSSIFVLSLLLIGVFVQTHAKNEQSATFSYEGSNGPEHWGSMSSKFATCSNGKSQS 60

Query: 61  PIDIVKDKTSFGKHLQTLIRKYTSANATLVNNGFNIGLHFGENSGGVAVIDGKNYSLKQM 120
           PIDIVKD   FGKHLQ L+R+Y +ANATLVNNGFNIG+ FGE +GGVAVID KNY+L+Q+
Sbjct: 61  PIDIVKDGAVFGKHLQKLVRRYNTANATLVNNGFNIGVDFGEKTGGVAVIDDKNYTLRQL 120

Query: 121 HWHSPSEHRLNGEQFAAELHLLHQADDGSLSVIAILLQYGDPDLLLQKIQDKLGELAKER 180
           HWHSPSEH LNGE+FAAELHL+H+ADDG LSVIAILLQ GD D LL+KIQDKL EL KE+
Sbjct: 121 HWHSPSEHLLNGERFAAELHLIHKADDGGLSVIAILLQDGDSDPLLEKIQDKLRELTKEK 180

Query: 181 CGADEESHITIENLDTRQLRKKTRKYYRYVGSLTTPPCSENVIWSILGKVRTISKEQVEA 240
           CG++EE+HI +  L+ + LRKKTRKYYRY+GS T PPC+ENVIW+ILGKVRTISKEQVEA
Sbjct: 181 CGSNEEAHIALGELNLKHLRKKTRKYYRYIGSFTVPPCTENVIWTILGKVRTISKEQVEA 240

Query: 241 LKAPLDSAYKNNARPAQALNGRKIEIYDELSEY 273
           LKAPL+  YK NARPAQ LNGRKIEIYDELSEY
Sbjct: 241 LKAPLEPPYKKNARPAQPLNGRKIEIYDELSEY 273

[Close]

BLAST of MS019525 vs. TAIR 10
Match: AT3G52720.1 (alpha carbonic anhydrase 1 )

HSP 1 Score: 270.0 bits (689), Expect = 2.1e-72
Identity = 128/258 (49.61%), Postives = 183/258 (70.93%), Query Frame = 0


Query: 11  FSLLFIGVFVQADQVGQSAAFTYDGSHGPQYWGELSPSFAACKNGKSQSPIDIVKDKTSF 70
           FS+  I +   AD   +   F Y G +GP  WG L+P F  C  GK QSPIDI + +  +
Sbjct: 12  FSMSLICI-APADAQTEGVVFGYKGKNGPNQWGHLNPHFTTCAVGKLQSPIDIQRRQIFY 71

Query: 71  GKHLQTLIRKYTSANATLVNNGFNIGLHFGENSGGVAVIDGKNYSLKQMHWHSPSEHRLN 130
              L ++ R+Y   NATLVN+  N+ + FGE +G V +I+ KNY+L QMHWH+PSEH L+
Sbjct: 72  NHKLNSIHREYYFTNATLVNHVCNVAMFFGEGAGDV-IIENKNYTLLQMHWHTPSEHHLH 131

Query: 131 GEQFAAELHLLHQADDGSLSVIAILLQYGDPDLLLQKIQDKLGELAKERCGADEESHITI 190
           G Q+AAELH++HQA DGS +V+A L + G  +  L ++++KL +L +ER   +  + + +
Sbjct: 132 GVQYAAELHMVHQAKDGSFAVVASLFKIGTEEPFLSQMKEKLVKLKEERLKGNHTAQVEV 191

Query: 191 ENLDTRQLRKKTRKYYRYVGSLTTPPCSENVIWSILGKVRTISKEQVEALKAPLDSAYKN 250
             +DTR + +KTRKYYRY+GSLTTPPCSENV W+ILGKVR++SKEQVE L++PLD+++KN
Sbjct: 192 GRIDTRHIERKTRKYYRYIGSLTTPPCSENVSWTILGKVRSMSKEQVELLRSPLDTSFKN 251

Query: 251 NARPAQALNGRKIEIYDE 269
           N+RP Q LNGR++E++ +
Sbjct: 252 NSRPCQPLNGRRVEMFHD 267

[Close]

BLAST of MS019525 vs. TAIR 10
Match: AT3G52720.2 (alpha carbonic anhydrase 1 )

HSP 1 Score: 219.2 bits (557), Expect = 4.2e-57
Identity = 106/219 (48.40%), Postives = 148/219 (67.58%), Query Frame = 0


Query: 11  FSLLFIGVFVQADQVGQSAAFTYDGSHGPQYWGELSPSFAACKNGKSQSPIDIVKDKTSF 70
           FS+  I +   AD   +   F Y G +GP  WG L+P F  C  GK QSPIDI + +  +
Sbjct: 12  FSMSLICI-APADAQTEGVVFGYKGKNGPNQWGHLNPHFTTCAVGKLQSPIDIQRRQIFY 71

Query: 71  GKHLQTLIRKYTSANATLVNNGFNIGLHFGENSGGVAVIDGKNYSLKQMHWHSPSEHRLN 130
              L ++ R+Y   NATLVN+  N+ + FGE +G V +I+ KNY+L QMHWH+PSEH L+
Sbjct: 72  NHKLNSIHREYYFTNATLVNHVCNVAMFFGEGAGDV-IIENKNYTLLQMHWHTPSEHHLH 131

Query: 131 GEQFAAELHLLHQADDGSLSVIAILLQYGDPDLLLQKIQDKLGELAKERCGADEESHITI 190
           G Q+AAELH++HQA DGS +V+A L + G  +  L ++++KL +L +ER   +  + + +
Sbjct: 132 GVQYAAELHMVHQAKDGSFAVVASLFKIGTEEPFLSQMKEKLVKLKEERLKGNHTAQVEV 191

Query: 191 ENLDTRQLRKKTRKYYRYVGSLTTPPCSENVIWSILGKV 230
             +DTR + +KTRKYYRY+GSLTTPPCSENV W+ILGKV
Sbjct: 192 GRIDTRHIERKTRKYYRYIGSLTTPPCSENVSWTILGKV 228

[Close]

BLAST of MS019525 vs. TAIR 10
Match: AT1G08080.1 (alpha carbonic anhydrase 7 )

HSP 1 Score: 197.2 bits (500), Expect = 1.7e-50
Identity = 96/245 (39.18%), Postives = 151/245 (61.63%), Query Frame = 0


Query: 24  QVGQSAAFTY--DGSHGPQYWGELSPSFAACKNGKSQSPIDIVKDKTSFGKHLQTLIRKY 83
           +V     F Y  +   GP+ WGEL P +  C  G+ QSPID++ ++ +   HL  L R Y
Sbjct: 33  EVEDEREFNYKKNDEKGPERWGELKPEWEMCGKGEMQSPIDLMNERVNIVSHLGRLNRDY 92

Query: 84  TSANATLVNNGFNIGLHFGENSGGVAVIDGKNYSLKQMHWHSPSEHRLNGEQFAAELHLL 143
             +NATL N G +I L F E+  G   I+G  Y L+Q+HWHSPSEH +NG +FA ELH++
Sbjct: 93  NPSNATLKNRGHDIMLKF-EDGAGTIKINGFEYELQQLHWHSPSEHTINGRRFALELHMV 152

Query: 144 HQADDGSLSVIAILLQYGDPDLLLQKIQDKLGELAKERCGADEESHITIENLDTRQLRKK 203
           H+  +  ++V+ +L + G  D  ++ ++ +L  +A+      EE+   +  +D  +++  
Sbjct: 153 HEGRNRRMAVVTVLYKIGRADTFIRSLEKELEGIAEM-----EEAEKNVGMIDPTKIKIG 212

Query: 204 TRKYYRYVGSLTTPPCSENVIWSILGKVRTISKEQVEALKAPLDSAYKNNARPAQALNGR 263
           +RKYYRY GSLTTPPC++NV WS++ KVRT++++QV+ L+  +     +NARP Q  N R
Sbjct: 213 SRKYYRYTGSLTTPPCTQNVTWSVVRKVRTVTRKQVKLLRVAVHDDANSNARPVQPTNKR 271

Query: 264 KIEIY 267
            + +Y
Sbjct: 273 IVHLY 271

[Close]

BLAST of MS019525 vs. TAIR 10
Match: AT1G08065.1 (alpha carbonic anhydrase 5 )

HSP 1 Score: 186.8 bits (473), Expect = 2.3e-47
Identity = 101/266 (37.97%), Postives = 152/266 (57.14%), Query Frame = 0


Query: 9   FVFSLLFIGVFVQAD-----QVGQSAAFTYD--GSHGPQYWGELSPSFAACKNGKSQSPI 68
           +VF L+FI + + +      +V     F Y+  G  GP+ WG L P +A C  G  QSPI
Sbjct: 8   YVFFLIFISITIVSSSPDHGEVEDETQFNYEKKGEKGPENWGRLKPEWAMCGKGNMQSPI 67

Query: 69  DIVKDKTSFGKHLQTLIRKYTSANATLVNNGFNIGLHF-GENSGGVAVIDGKNYSLKQMH 128
           D+   +     +L  L  +Y  +NAT+ N G +I + F G N+G    I+G  Y L+Q+H
Sbjct: 68  DLTDKRVLIDHNLGYLRSQYLPSNATIKNRGHDIMMKFEGGNAGLGITINGTEYKLQQIH 127

Query: 129 WHSPSEHRLNGEQFAAELHLLHQADDGSLSVIAILLQYGDPDLLLQKIQDKLGELAKERC 188
           WHSPSEH LNG++F  E H++HQ+ DG  +V+A   + G PD  L  ++  L     +R 
Sbjct: 128 WHSPSEHTLNGKRFVLEEHMVHQSKDGRNAVVAFFYKLGKPDYFLLTLERYL-----KRI 187

Query: 189 GADEESHITIENLDTRQLRKKTRKYYRYVGSLTTPPCSENVIWSILGKVRTISKEQVEAL 248
               ES   +E +  R    +++ YYR++GSLTTPPCSENVIW+I  ++RT++ +Q+  L
Sbjct: 188 TDTHESQEFVEMVHPRTFGFESKHYYRFIGSLTTPPCSENVIWTISKEMRTVTLKQLIML 247

Query: 249 KAPLDSAYKNNARPAQALNGRKIEIY 267
           +  +     +NARP Q  N R + +Y
Sbjct: 248 RVTVHDQSNSNARPLQRKNERPVALY 268

[Close]

BLAST of MS019525 vs. TAIR 10
Match: AT2G28210.1 (alpha carbonic anhydrase 2 )

HSP 1 Score: 183.0 bits (463), Expect = 3.3e-46
Identity = 83/202 (41.09%), Postives = 129/202 (63.86%), Query Frame = 0


Query: 30  AFTYDGSHGPQYWGELSPSFAACKNGKSQSPIDIVKDKTSFGKHLQTLIRKYTSANATLV 89
           ++ ++  +GP  WG+L P +  C  G+ QSPID++  +     HL+ L R Y   NATL 
Sbjct: 12  SYEWNQENGPAKWGKLRPEWKMCGKGEMQSPIDLMNKRVRLVTHLKKLTRHYKPCNATLK 71

Query: 90  NNGFNIGLHFGENSGGVAVIDGKNYSLKQMHWHSPSEHRLNGEQFAAELHLLHQADDGSL 149
           N G ++ L FGE   G   ++G  Y L Q+HWHSPSEH +NG +FA ELH++H+  +GSL
Sbjct: 72  NRGHDMMLKFGEEGSGSITVNGTEYKLLQLHWHSPSEHTMNGRRFALELHMVHENINGSL 131

Query: 150 SVIAILLQYGDPDLLLQKIQDKLGELAKERCGADEESHITIENLDTRQLRKKTRKYYRYV 209
           +V+ +L + G PD  L  +++KL  +  +      E+   ++ +D R ++  +RK+YRY+
Sbjct: 132 AVVTVLYKIGRPDSFLGLLENKLSAITDQ-----NEAEKYVDVIDPRDIKIGSRKFYRYI 191

Query: 210 GSLTTPPCSENVIWSILGKVRT 232
           GSLTTPPC++NVIW+++ KV T
Sbjct: 192 GSLTTPPCTQNVIWTVVKKVNT 208

The following BLAST results are available for this feature:

BLAST of MS019525 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022144656.1	5.5e-152	100.00	alpha carbonic anhydrase 1, chloroplastic [Momordica charantia]	[more]
XP_004149223.1	3.0e-118	76.47	alpha carbonic anhydrase 1, chloroplastic isoform X2 [Cucumis sativus]	[more]
XP_038903044.1	5.2e-118	76.56	alpha carbonic anhydrase 1, chloroplastic [Benincasa hispida]	[more]
XP_011652015.1	1.2e-117	76.56	alpha carbonic anhydrase 1, chloroplastic isoform X1 [Cucumis sativus] >KGN59137...	[more]
XP_008442846.1	4.9e-116	74.73	PREDICTED: alpha carbonic anhydrase 1, chloroplastic [Cucumis melo] >KAA0043878....	[more]

BLAST of MS019525 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04846	2.9e-71	49.61	Alpha carbonic anhydrase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ACA...	[more]
Q8L817	2.4e-49	39.18	Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana OX=3702 GN=ACA7 PE=2 SV=1	[more]
F4HUC4	3.2e-46	37.97	Alpha carbonic anhydrase 5 OS=Arabidopsis thaliana OX=3702 GN=ACA5 PE=3 SV=1	[more]
Q9FM99	4.7e-45	39.84	Alpha carbonic anhydrase 8 OS=Arabidopsis thaliana OX=3702 GN=ACA8 PE=3 SV=3	[more]
F4IHR4	8.0e-45	39.11	Alpha carbonic anhydrase 2 OS=Arabidopsis thaliana OX=3702 GN=ACA2 PE=2 SV=2	[more]

BLAST of MS019525 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CU20	2.7e-152	100.00	Carbonic anhydrase OS=Momordica charantia OX=3673 GN=LOC111014291 PE=3 SV=1	[more]
A0A0A0LEL1	5.6e-118	76.56	Alpha-carbonic anhydrase domain-containing protein OS=Cucumis sativus OX=3659 GN...	[more]
A0A5A7TLZ2	2.4e-116	74.73	Alpha carbonic anhydrase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A1S3B6Q0	2.4e-116	74.73	alpha carbonic anhydrase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10348661...	[more]
A0A6J1J129	1.3e-111	73.99	Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111481722 PE=3 SV=1	[more]

BLAST of MS019525 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G52720.1	2.1e-72	49.61	alpha carbonic anhydrase 1	[more]
AT3G52720.2	4.2e-57	48.40	alpha carbonic anhydrase 1	[more]
AT1G08080.1	1.7e-50	39.18	alpha carbonic anhydrase 7	[more]
AT1G08065.1	2.3e-47	37.97	alpha carbonic anhydrase 5	[more]
AT2G28210.1	3.3e-46	41.09	alpha carbonic anhydrase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001148	Alpha carbonic anhydrase domain	SMART	SM01057	Carb_anhydrase_2a	coord: 31..267 e-value: 7.0E-73 score: 258.1
IPR001148	Alpha carbonic anhydrase domain	PFAM	PF00194	Carb_anhydrase	coord: 31..264 e-value: 1.2E-44 score: 152.8
IPR001148	Alpha carbonic anhydrase domain	PROSITE	PS51144	ALPHA_CA_2	coord: 29..267 score: 49.285942
IPR036398	Alpha carbonic anhydrase domain superfamily	GENE3D	3.10.200.10	Alpha carbonic anhydrase	coord: 27..271 e-value: 3.3E-72 score: 244.8
IPR036398	Alpha carbonic anhydrase domain superfamily	SUPERFAMILY	51069	Carbonic anhydrase	coord: 30..263
None	No IPR available	PANTHER	PTHR18952:SF243	OS04G0412500 PROTEIN	coord: 13..269
IPR023561	Carbonic anhydrase, alpha-class	PANTHER	PTHR18952	CARBONIC ANHYDRASE	coord: 13..269
IPR018338	Carbonic anhydrase, alpha-class, conserved site	PROSITE	PS00162	ALPHA_CA_1	coord: 125..141
IPR041891	Carbonic anhydrase, prokaryotic-like	CDD	cd03124	alpha_CA_prokaryotic_like	coord: 38..264 e-value: 3.23257E-96 score: 279.927

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS019525.1	MS019525.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004089	carbonate dehydratase activity
molecular_function	GO:0008270	zinc ion binding