MS019471 (gene) Bitter gourd (TR) v1

Overview
NameMS019471
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionUbiquitin-conjugating enzyme E2 variant 1D
Locationscaffold28: 747520 .. 748512 (-)
RNA-Seq ExpressionMS019471
SyntenyMS019471
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GCATCTAACAAGTGTTTACTTTCCATCACAGTTCCTAGGAACTTCAGGTTGCTGGAGGAACTTGAACGTGGAGAAAAGGGAATTGGCGATGGCACTGTGAGCTATGGAATGGATGATGGAGATGACATTTATATGCGTTCATGGACTGGCACCATCATTGGCCCACACAATGTAATGGCTTCTGCTCTTCTCTTCAGGAGTTTTAATCGCTTGCTCATGAAGAAAATTTGAAAATTTACACTACATTAACTACCACTCAAATTAAACTTGCTCTTTTCCGTTTGTAATTAGCATAATATTTTTTTTGCCTTGAAGTCTGGCGAAATGCTCTTACTTTTAAATGGTTGATAAAGAGGTGCTGTTAGGGAATCTTATGCTAAAAATTGGAAATGTCTACTTTATTTTACAATTCACAATTCCTATTATAAGGCGACACCTACAGTGCATTAAAAATTCATAATGCTTCTTTTTTCCTTGCAGACGGTACATGAAGGTAGAATTTATCAGTTGAAGCTGTTCTGTGATAAAGATTACCCTGATAAGCCACCAAGTGTTCGTTTTCATTCCCGAGTCAACATGACATGCGTGAACCATGAAACTGGAGTGGTATCTCTGATTTCACTCCCTCAGTTGATAATATGCATATCTTAGTACTTTATGTTGAACTAAAAGTTGACGTTTATAAGAAGACCAAAAGTTTTCATTTACCTGCAAATACATAGATTACCTCTGACAGCAGCCCACCGTCATGGATATCATTTACCTACAACTACATAGATGATTTTATCAACCAATCTTGCTCTTCTTCTCTTTGAAACTGATGAATTTCTACGTTATTAATTGATTTCCCAACAGGTCGAGTCGAAGAAATTTGGACTTCTTGCAAATTGGCAGCGAGAGTACACCATGGAGGATATACTCACACAGCTGAAAAAGGAAATGGCTGCCCCACATAACCGAAAGCTCGTCCAACCTCCAGAGGGCACCTACTTC

mRNA sequence

GCATCTAACAAGTGTTTACTTTCCATCACAGTTCCTAGGAACTTCAGGTTGCTGGAGGAACTTGAACGTGGAGAAAAGGGAATTGGCGATGGCACTGTGAGCTATGGAATGGATGATGGAGATGACATTTATATGCGTTCATGGACTGGCACCATCATTGGCCCACACAATACGGTACATGAAGGTAGAATTTATCAGTTGAAGCTGTTCTGTGATAAAGATTACCCTGATAAGCCACCAAGTGTTCGTTTTCATTCCCGAGTCAACATGACATGCGTGAACCATGAAACTGGAGTGGTCGAGTCGAAGAAATTTGGACTTCTTGCAAATTGGCAGCGAGAGTACACCATGGAGGATATACTCACACAGCTGAAAAAGGAAATGGCTGCCCCACATAACCGAAAGCTCGTCCAACCTCCAGAGGGCACCTACTTC

Coding sequence (CDS)

GCATCTAACAAGTGTTTACTTTCCATCACAGTTCCTAGGAACTTCAGGTTGCTGGAGGAACTTGAACGTGGAGAAAAGGGAATTGGCGATGGCACTGTGAGCTATGGAATGGATGATGGAGATGACATTTATATGCGTTCATGGACTGGCACCATCATTGGCCCACACAATACGGTACATGAAGGTAGAATTTATCAGTTGAAGCTGTTCTGTGATAAAGATTACCCTGATAAGCCACCAAGTGTTCGTTTTCATTCCCGAGTCAACATGACATGCGTGAACCATGAAACTGGAGTGGTCGAGTCGAAGAAATTTGGACTTCTTGCAAATTGGCAGCGAGAGTACACCATGGAGGATATACTCACACAGCTGAAAAAGGAAATGGCTGCCCCACATAACCGAAAGCTCGTCCAACCTCCAGAGGGCACCTACTTC

Protein sequence

ASNKCLLSITVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLFCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAAPHNRKLVQPPEGTYF
Homology
BLAST of MS019471 vs. NCBI nr
Match: KAG7028134.1 (Ubiquitin-conjugating enzyme E2 variant 1D [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 302.0 bits (772), Expect = 2.8e-78
Identity = 140/145 (96.55%), Postives = 142/145 (97.93%), Query Frame = 0

Query: 1   ASNKCLLSITVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVH 60
           AS KCLLSITVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVH
Sbjct: 12  ASFKCLLSITVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVH 71

Query: 61  EGRIYQLKLFCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDI 120
           EGRIYQLKLFCDKDYP+KPPSVRFHSRVNMTCVNHETGVVE KKFGLLANWQREY+MEDI
Sbjct: 72  EGRIYQLKLFCDKDYPEKPPSVRFHSRVNMTCVNHETGVVEPKKFGLLANWQREYSMEDI 131

Query: 121 LTQLKKEMAAPHNRKLVQPPEGTYF 146
           LTQLKKEMAAP NRKLVQPPEGTYF
Sbjct: 132 LTQLKKEMAAPQNRKLVQPPEGTYF 156

BLAST of MS019471 vs. NCBI nr
Match: XP_022144653.1 (ubiquitin-conjugating enzyme E2 variant 1C [Momordica charantia] >XP_038904037.1 ubiquitin-conjugating enzyme E2 variant 1C [Benincasa hispida])

HSP 1 Score: 297.0 bits (759), Expect = 9.0e-77
Identity = 136/138 (98.55%), Postives = 137/138 (99.28%), Query Frame = 0

Query: 8   SITVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL 67
           S+ VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL
Sbjct: 9   SVVVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL 68

Query: 68  KLFCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKE 127
           KLFCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKE
Sbjct: 69  KLFCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKE 128

Query: 128 MAAPHNRKLVQPPEGTYF 146
           MAAPHNRKLVQPPEGTYF
Sbjct: 129 MAAPHNRKLVQPPEGTYF 146

BLAST of MS019471 vs. NCBI nr
Match: XP_004136503.1 (ubiquitin-conjugating enzyme E2 variant 1C [Cucumis sativus] >XP_008442892.1 PREDICTED: ubiquitin-conjugating enzyme E2 variant 1C [Cucumis melo] >KGN59179.1 hypothetical protein Csa_001903 [Cucumis sativus])

HSP 1 Score: 295.4 bits (755), Expect = 2.6e-76
Identity = 135/138 (97.83%), Postives = 137/138 (99.28%), Query Frame = 0

Query: 8   SITVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL 67
           S+ VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL
Sbjct: 9   SVVVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL 68

Query: 68  KLFCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKE 127
           KLFCDKDYP+KPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKE
Sbjct: 69  KLFCDKDYPEKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKE 128

Query: 128 MAAPHNRKLVQPPEGTYF 146
           MAAPHNRKLVQPPEGTYF
Sbjct: 129 MAAPHNRKLVQPPEGTYF 146

BLAST of MS019471 vs. NCBI nr
Match: XP_022983154.1 (ubiquitin-conjugating enzyme E2 variant 1C [Cucurbita maxima])

HSP 1 Score: 295.4 bits (755), Expect = 2.6e-76
Identity = 135/138 (97.83%), Postives = 137/138 (99.28%), Query Frame = 0

Query: 8   SITVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL 67
           S+ VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL
Sbjct: 9   SVVVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL 68

Query: 68  KLFCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKE 127
           KLFCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREY+MEDILTQLKKE
Sbjct: 69  KLFCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYSMEDILTQLKKE 128

Query: 128 MAAPHNRKLVQPPEGTYF 146
           MAAPHNRKLVQPPEGTYF
Sbjct: 129 MAAPHNRKLVQPPEGTYF 146

BLAST of MS019471 vs. NCBI nr
Match: XP_021636663.1 (ubiquitin-conjugating enzyme E2 variant 1C isoform X2 [Hevea brasiliensis] >XP_022766996.1 ubiquitin-conjugating enzyme E2 variant 1C [Durio zibethinus])

HSP 1 Score: 295.0 bits (754), Expect = 3.4e-76
Identity = 134/138 (97.10%), Postives = 137/138 (99.28%), Query Frame = 0

Query: 8   SITVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL 67
           S+ VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL
Sbjct: 9   SVVVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL 68

Query: 68  KLFCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKE 127
           KLFCDKDYP+KPPSVRFHSR+NMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKE
Sbjct: 69  KLFCDKDYPEKPPSVRFHSRINMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKE 128

Query: 128 MAAPHNRKLVQPPEGTYF 146
           MAAPHNRKLVQPPEGTYF
Sbjct: 129 MAAPHNRKLVQPPEGTYF 146

BLAST of MS019471 vs. ExPASy Swiss-Prot
Match: Q9SJ44 (Ubiquitin-conjugating enzyme E2 variant 1C OS=Arabidopsis thaliana OX=3702 GN=UEV1C PE=1 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 6.1e-76
Identity = 127/138 (92.03%), Postives = 136/138 (98.55%), Query Frame = 0

Query: 8   SITVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL 67
           S+ VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL
Sbjct: 8   SVVVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL 67

Query: 68  KLFCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKE 127
           KLFCDKDYP+KPP+VRFHSR+NMTCVNH+TGVV+SKKFG+LANWQR+YTMEDILTQLKKE
Sbjct: 68  KLFCDKDYPEKPPTVRFHSRINMTCVNHDTGVVDSKKFGVLANWQRQYTMEDILTQLKKE 127

Query: 128 MAAPHNRKLVQPPEGTYF 146
           MAA HNRKLVQPPEGT+F
Sbjct: 128 MAASHNRKLVQPPEGTFF 145

BLAST of MS019471 vs. ExPASy Swiss-Prot
Match: Q9SVD7 (Ubiquitin-conjugating enzyme E2 variant 1D OS=Arabidopsis thaliana OX=3702 GN=UEV1D PE=1 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 5.7e-74
Identity = 126/138 (91.30%), Postives = 131/138 (94.93%), Query Frame = 0

Query: 8   SITVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL 67
           S+ VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL
Sbjct: 9   SVVVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL 68

Query: 68  KLFCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKE 127
           KLFCDKDYP+KPP+VRFHSRVNM CVNHETGVV+ KKFGLLANWQREYTMEDIL QLKKE
Sbjct: 69  KLFCDKDYPEKPPTVRFHSRVNMACVNHETGVVDPKKFGLLANWQREYTMEDILVQLKKE 128

Query: 128 MAAPHNRKLVQPPEGTYF 146
           M+  HNRKLVQPPEGT F
Sbjct: 129 MSTSHNRKLVQPPEGTCF 146

BLAST of MS019471 vs. ExPASy Swiss-Prot
Match: Q93YP0 (Ubiquitin-conjugating enzyme E2 variant 1A OS=Arabidopsis thaliana OX=3702 GN=UEV1A PE=1 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 5.2e-59
Identity = 101/134 (75.37%), Postives = 115/134 (85.82%), Query Frame = 0

Query: 9   ITVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLK 68
           + VPRNFRLLEELERGEKGIGDGTVSYGMDD DDIYM+SWTGTI+GP NT +EG+I+QLK
Sbjct: 8   VVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDIYMQSWTGTILGPPNTAYEGKIFQLK 67

Query: 69  LFCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEM 128
           LFC K+YP+ PP+VRF +R+NM CVN ETGVVE   F +L NW+REYTMEDIL +LKKEM
Sbjct: 68  LFCGKEYPESPPTVRFQTRINMACVNPETGVVEPSLFPMLTNWRREYTMEDILVKLKKEM 127

Query: 129 AAPHNRKLVQPPEG 143
              HNRKL QPPEG
Sbjct: 128 MTSHNRKLAQPPEG 141

BLAST of MS019471 vs. ExPASy Swiss-Prot
Match: Q9CAB6 (Ubiquitin-conjugating enzyme E2 variant 1B OS=Arabidopsis thaliana OX=3702 GN=UEV1B PE=1 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 4.8e-57
Identity = 98/134 (73.13%), Postives = 114/134 (85.07%), Query Frame = 0

Query: 9   ITVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLK 68
           + VPRNFRLLEELERGEKGIGDGTVSYGMDD DDI M+SWTGTI+GPHNT +EG+I+QLK
Sbjct: 8   VVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDILMQSWTGTILGPHNTAYEGKIFQLK 67

Query: 69  LFCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEM 128
           LFC KDYP+ PP+VRF SR+NM CVN E GVV+   F +L+NW+RE+TMED+L QLKKEM
Sbjct: 68  LFCGKDYPESPPTVRFQSRINMACVNPENGVVDPSHFPMLSNWRREFTMEDLLIQLKKEM 127

Query: 129 AAPHNRKLVQPPEG 143
            +  NRKL QP EG
Sbjct: 128 MSSQNRKLAQPLEG 141

BLAST of MS019471 vs. ExPASy Swiss-Prot
Match: Q54D06 (Probable ubiquitin-conjugating enzyme E2 variant OS=Dictyostelium discoideum OX=44689 GN=ube2v PE=3 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 4.2e-45
Identity = 77/136 (56.62%), Postives = 105/136 (77.21%), Query Frame = 0

Query: 10  TVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKL 69
           T+PRNF LLEELE GEK  GDGT+SYG++  DDI + SW GTIIGP NT  + R Y LKL
Sbjct: 3   TIPRNFVLLEELESGEKSTGDGTISYGLESPDDILLSSWIGTIIGPQNTPFDNRFYSLKL 62

Query: 70  FCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMA 129
           +CDKDYP+KPP+VRF +++N+ CVN + GVV+ K   +L+NW  +  +++IL++L++EM+
Sbjct: 63  YCDKDYPNKPPTVRFTTKINIGCVNQQNGVVDPKNLPILSNWTSKNRIQNILSELRREMS 122

Query: 130 APHNRKLVQPPEGTYF 146
           +  N+KLVQPPEG  F
Sbjct: 123 SSSNKKLVQPPEGANF 138

BLAST of MS019471 vs. ExPASy TrEMBL
Match: A0A6J1CTV6 (ubiquitin-conjugating enzyme E2 variant 1C OS=Momordica charantia OX=3673 GN=LOC111014288 PE=4 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 4.4e-77
Identity = 136/138 (98.55%), Postives = 137/138 (99.28%), Query Frame = 0

Query: 8   SITVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL 67
           S+ VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL
Sbjct: 9   SVVVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL 68

Query: 68  KLFCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKE 127
           KLFCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKE
Sbjct: 69  KLFCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKE 128

Query: 128 MAAPHNRKLVQPPEGTYF 146
           MAAPHNRKLVQPPEGTYF
Sbjct: 129 MAAPHNRKLVQPPEGTYF 146

BLAST of MS019471 vs. ExPASy TrEMBL
Match: A0A6J1J6I4 (ubiquitin-conjugating enzyme E2 variant 1C OS=Cucurbita maxima OX=3661 GN=LOC111481792 PE=4 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 1.3e-76
Identity = 135/138 (97.83%), Postives = 137/138 (99.28%), Query Frame = 0

Query: 8   SITVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL 67
           S+ VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL
Sbjct: 9   SVVVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL 68

Query: 68  KLFCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKE 127
           KLFCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREY+MEDILTQLKKE
Sbjct: 69  KLFCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYSMEDILTQLKKE 128

Query: 128 MAAPHNRKLVQPPEGTYF 146
           MAAPHNRKLVQPPEGTYF
Sbjct: 129 MAAPHNRKLVQPPEGTYF 146

BLAST of MS019471 vs. ExPASy TrEMBL
Match: A0A0A0LE42 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G778350 PE=4 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 1.3e-76
Identity = 135/138 (97.83%), Postives = 137/138 (99.28%), Query Frame = 0

Query: 8   SITVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL 67
           S+ VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL
Sbjct: 9   SVVVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL 68

Query: 68  KLFCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKE 127
           KLFCDKDYP+KPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKE
Sbjct: 69  KLFCDKDYPEKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKE 128

Query: 128 MAAPHNRKLVQPPEGTYF 146
           MAAPHNRKLVQPPEGTYF
Sbjct: 129 MAAPHNRKLVQPPEGTYF 146

BLAST of MS019471 vs. ExPASy TrEMBL
Match: A0A1S3B6A6 (ubiquitin-conjugating enzyme E2 variant 1C OS=Cucumis melo OX=3656 GN=LOC103486656 PE=4 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 1.3e-76
Identity = 135/138 (97.83%), Postives = 137/138 (99.28%), Query Frame = 0

Query: 8   SITVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL 67
           S+ VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL
Sbjct: 9   SVVVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL 68

Query: 68  KLFCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKE 127
           KLFCDKDYP+KPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKE
Sbjct: 69  KLFCDKDYPEKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKE 128

Query: 128 MAAPHNRKLVQPPEGTYF 146
           MAAPHNRKLVQPPEGTYF
Sbjct: 129 MAAPHNRKLVQPPEGTYF 146

BLAST of MS019471 vs. ExPASy TrEMBL
Match: A0A6P6AQA1 (ubiquitin-conjugating enzyme E2 variant 1C OS=Durio zibethinus OX=66656 GN=LOC111311670 PE=4 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.7e-76
Identity = 134/138 (97.10%), Postives = 137/138 (99.28%), Query Frame = 0

Query: 8   SITVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL 67
           S+ VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL
Sbjct: 9   SVVVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL 68

Query: 68  KLFCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKE 127
           KLFCDKDYP+KPPSVRFHSR+NMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKE
Sbjct: 69  KLFCDKDYPEKPPSVRFHSRINMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKE 128

Query: 128 MAAPHNRKLVQPPEGTYF 146
           MAAPHNRKLVQPPEGTYF
Sbjct: 129 MAAPHNRKLVQPPEGTYF 146

BLAST of MS019471 vs. TAIR 10
Match: AT2G36060.1 (MMS ZWEI homologue 3 )

HSP 1 Score: 284.6 bits (727), Expect = 4.3e-77
Identity = 127/138 (92.03%), Postives = 136/138 (98.55%), Query Frame = 0

Query: 8   SITVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL 67
           S+ VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL
Sbjct: 8   SVVVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL 67

Query: 68  KLFCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKE 127
           KLFCDKDYP+KPP+VRFHSR+NMTCVNH+TGVV+SKKFG+LANWQR+YTMEDILTQLKKE
Sbjct: 68  KLFCDKDYPEKPPTVRFHSRINMTCVNHDTGVVDSKKFGVLANWQRQYTMEDILTQLKKE 127

Query: 128 MAAPHNRKLVQPPEGTYF 146
           MAA HNRKLVQPPEGT+F
Sbjct: 128 MAASHNRKLVQPPEGTFF 145

BLAST of MS019471 vs. TAIR 10
Match: AT2G36060.2 (MMS ZWEI homologue 3 )

HSP 1 Score: 280.0 bits (715), Expect = 1.1e-75
Identity = 127/139 (91.37%), Postives = 136/139 (97.84%), Query Frame = 0

Query: 8   SITVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHN-TVHEGRIYQ 67
           S+ VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHN TVHEGRIYQ
Sbjct: 8   SVVVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNVTVHEGRIYQ 67

Query: 68  LKLFCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKK 127
           LKLFCDKDYP+KPP+VRFHSR+NMTCVNH+TGVV+SKKFG+LANWQR+YTMEDILTQLKK
Sbjct: 68  LKLFCDKDYPEKPPTVRFHSRINMTCVNHDTGVVDSKKFGVLANWQRQYTMEDILTQLKK 127

Query: 128 EMAAPHNRKLVQPPEGTYF 146
           EMAA HNRKLVQPPEGT+F
Sbjct: 128 EMAASHNRKLVQPPEGTFF 146

BLAST of MS019471 vs. TAIR 10
Match: AT2G36060.3 (MMS ZWEI homologue 3 )

HSP 1 Score: 278.1 bits (710), Expect = 4.0e-75
Identity = 126/138 (91.30%), Postives = 135/138 (97.83%), Query Frame = 0

Query: 8   SITVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL 67
           S+ VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL
Sbjct: 8   SVVVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL 67

Query: 68  KLFCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKE 127
           KLFCDKDYP+KPP+VRFHSR+NMTCVNH+TG V+SKKFG+LANWQR+YTMEDILTQLKKE
Sbjct: 68  KLFCDKDYPEKPPTVRFHSRINMTCVNHDTG-VDSKKFGVLANWQRQYTMEDILTQLKKE 127

Query: 128 MAAPHNRKLVQPPEGTYF 146
           MAA HNRKLVQPPEGT+F
Sbjct: 128 MAASHNRKLVQPPEGTFF 144

BLAST of MS019471 vs. TAIR 10
Match: AT3G52560.1 (ubiquitin E2 variant 1D-4 )

HSP 1 Score: 278.1 bits (710), Expect = 4.0e-75
Identity = 126/138 (91.30%), Postives = 131/138 (94.93%), Query Frame = 0

Query: 8   SITVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL 67
           S+ VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL
Sbjct: 9   SVVVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQL 68

Query: 68  KLFCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKE 127
           KLFCDKDYP+KPP+VRFHSRVNM CVNHETGVV+ KKFGLLANWQREYTMEDIL QLKKE
Sbjct: 69  KLFCDKDYPEKPPTVRFHSRVNMACVNHETGVVDPKKFGLLANWQREYTMEDILVQLKKE 128

Query: 128 MAAPHNRKLVQPPEGTYF 146
           M+  HNRKLVQPPEGT F
Sbjct: 129 MSTSHNRKLVQPPEGTCF 146

BLAST of MS019471 vs. TAIR 10
Match: AT3G52560.2 (ubiquitin E2 variant 1D-4 )

HSP 1 Score: 273.5 bits (698), Expect = 9.9e-74
Identity = 126/139 (90.65%), Postives = 131/139 (94.24%), Query Frame = 0

Query: 8   SITVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHN-TVHEGRIYQ 67
           S+ VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHN TVHEGRIYQ
Sbjct: 9   SVVVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNVTVHEGRIYQ 68

Query: 68  LKLFCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKK 127
           LKLFCDKDYP+KPP+VRFHSRVNM CVNHETGVV+ KKFGLLANWQREYTMEDIL QLKK
Sbjct: 69  LKLFCDKDYPEKPPTVRFHSRVNMACVNHETGVVDPKKFGLLANWQREYTMEDILVQLKK 128

Query: 128 EMAAPHNRKLVQPPEGTYF 146
           EM+  HNRKLVQPPEGT F
Sbjct: 129 EMSTSHNRKLVQPPEGTCF 147

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7028134.12.8e-7896.55Ubiquitin-conjugating enzyme E2 variant 1D [Cucurbita argyrosperma subsp. argyro... [more]
XP_022144653.19.0e-7798.55ubiquitin-conjugating enzyme E2 variant 1C [Momordica charantia] >XP_038904037.1... [more]
XP_004136503.12.6e-7697.83ubiquitin-conjugating enzyme E2 variant 1C [Cucumis sativus] >XP_008442892.1 PRE... [more]
XP_022983154.12.6e-7697.83ubiquitin-conjugating enzyme E2 variant 1C [Cucurbita maxima][more]
XP_021636663.13.4e-7697.10ubiquitin-conjugating enzyme E2 variant 1C isoform X2 [Hevea brasiliensis] >XP_0... [more]
Match NameE-valueIdentityDescription
Q9SJ446.1e-7692.03Ubiquitin-conjugating enzyme E2 variant 1C OS=Arabidopsis thaliana OX=3702 GN=UE... [more]
Q9SVD75.7e-7491.30Ubiquitin-conjugating enzyme E2 variant 1D OS=Arabidopsis thaliana OX=3702 GN=UE... [more]
Q93YP05.2e-5975.37Ubiquitin-conjugating enzyme E2 variant 1A OS=Arabidopsis thaliana OX=3702 GN=UE... [more]
Q9CAB64.8e-5773.13Ubiquitin-conjugating enzyme E2 variant 1B OS=Arabidopsis thaliana OX=3702 GN=UE... [more]
Q54D064.2e-4556.62Probable ubiquitin-conjugating enzyme E2 variant OS=Dictyostelium discoideum OX=... [more]
Match NameE-valueIdentityDescription
A0A6J1CTV64.4e-7798.55ubiquitin-conjugating enzyme E2 variant 1C OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1J6I41.3e-7697.83ubiquitin-conjugating enzyme E2 variant 1C OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A0A0LE421.3e-7697.83UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G778350 PE... [more]
A0A1S3B6A61.3e-7697.83ubiquitin-conjugating enzyme E2 variant 1C OS=Cucumis melo OX=3656 GN=LOC1034866... [more]
A0A6P6AQA11.7e-7697.10ubiquitin-conjugating enzyme E2 variant 1C OS=Durio zibethinus OX=66656 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT2G36060.14.3e-7792.03MMS ZWEI homologue 3 [more]
AT2G36060.21.1e-7591.37MMS ZWEI homologue 3 [more]
AT2G36060.34.0e-7591.30MMS ZWEI homologue 3 [more]
AT3G52560.14.0e-7591.30ubiquitin E2 variant 1D-4 [more]
AT3G52560.29.9e-7490.65ubiquitin E2 variant 1D-4 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00212ubc_7coord: 15..143
e-value: 9.3E-5
score: 31.2
NoneNo IPR availablePANTHERPTHR24068UBIQUITIN-CONJUGATING ENZYME E2coord: 9..139
NoneNo IPR availablePANTHERPTHR24068:SF318UBIQUITIN-CONJUGATING ENZYME/RWD-LIKE PROTEIN-RELATEDcoord: 9..139
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 2..145
e-value: 7.3E-43
score: 147.6
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 10..145
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 42..135
e-value: 2.0E-14
score: 53.5
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 12..145
score: 31.934349

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS019471.1MS019471.1mRNA