MS019345 (gene) Bitter gourd (TR) v1

Overview
NameMS019345
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionpectinesterase inhibitor-like
Locationscaffold611: 510149 .. 510676 (+)
RNA-Seq ExpressionMS019345
SyntenyMS019345
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAACTGCAGTGTCGTGGCATCCTCCCTCTTGGCTTTCGTTTCTTCCCTTGTTTTGTTGTTGCATGTGGCATCCGCAAACGACGTCGCTTCCACCATCTGCCCGAAAACCAAAAACCCGGGCTTCTGCACGACCGAGTTGAAAACCGCGGGCAGCGCAGACCTCAAAGCCCTGGCCACCCACACCCTGAATCTCGCCCGTAAGAAGGCCGGCGAATCCGTGGCTCTGGCCAACGACCTTGCCGCCAAAGCCACCGATCCTCAGCTCAAGGAACGCTACACCTCCTGCTCCAAAAACTTCGACGAAGCCATCGGCAACATCGAAACCGCCGAGAAGCACTTGGCCTCCGGCGACTATAACGGCGTCAACCTTGCCGGCTCCGCCGTCATGTCCACCGTCGACGACTGTCGGGCCAACTTCGAGACTGGCCCGGCCGACACGTCGTCGCTCTCCACTAACGGCAAGACTCTGGATGATATTTCTAGCATTATCTTGGTGATTTCCAATCTTCTCCTTCAGCCCGCG

mRNA sequence

ATGGCAAACTGCAGTGTCGTGGCATCCTCCCTCTTGGCTTTCGTTTCTTCCCTTGTTTTGTTGTTGCATGTGGCATCCGCAAACGACGTCGCTTCCACCATCTGCCCGAAAACCAAAAACCCGGGCTTCTGCACGACCGAGTTGAAAACCGCGGGCAGCGCAGACCTCAAAGCCCTGGCCACCCACACCCTGAATCTCGCCCGTAAGAAGGCCGGCGAATCCGTGGCTCTGGCCAACGACCTTGCCGCCAAAGCCACCGATCCTCAGCTCAAGGAACGCTACACCTCCTGCTCCAAAAACTTCGACGAAGCCATCGGCAACATCGAAACCGCCGAGAAGCACTTGGCCTCCGGCGACTATAACGGCGTCAACCTTGCCGGCTCCGCCGTCATGTCCACCGTCGACGACTGTCGGGCCAACTTCGAGACTGGCCCGGCCGACACGTCGTCGCTCTCCACTAACGGCAAGACTCTGGATGATATTTCTAGCATTATCTTGGTGATTTCCAATCTTCTCCTTCAGCCCGCG

Coding sequence (CDS)

ATGGCAAACTGCAGTGTCGTGGCATCCTCCCTCTTGGCTTTCGTTTCTTCCCTTGTTTTGTTGTTGCATGTGGCATCCGCAAACGACGTCGCTTCCACCATCTGCCCGAAAACCAAAAACCCGGGCTTCTGCACGACCGAGTTGAAAACCGCGGGCAGCGCAGACCTCAAAGCCCTGGCCACCCACACCCTGAATCTCGCCCGTAAGAAGGCCGGCGAATCCGTGGCTCTGGCCAACGACCTTGCCGCCAAAGCCACCGATCCTCAGCTCAAGGAACGCTACACCTCCTGCTCCAAAAACTTCGACGAAGCCATCGGCAACATCGAAACCGCCGAGAAGCACTTGGCCTCCGGCGACTATAACGGCGTCAACCTTGCCGGCTCCGCCGTCATGTCCACCGTCGACGACTGTCGGGCCAACTTCGAGACTGGCCCGGCCGACACGTCGTCGCTCTCCACTAACGGCAAGACTCTGGATGATATTTCTAGCATTATCTTGGTGATTTCCAATCTTCTCCTTCAGCCCGCG

Protein sequence

MANCSVVASSLLAFVSSLVLLLHVASANDVASTICPKTKNPGFCTTELKTAGSADLKALATHTLNLARKKAGESVALANDLAAKATDPQLKERYTSCSKNFDEAIGNIETAEKHLASGDYNGVNLAGSAVMSTVDDCRANFETGPADTSSLSTNGKTLDDISSIILVISNLLLQPA
Homology
BLAST of MS019345 vs. NCBI nr
Match: XP_022138606.1 (pectinesterase inhibitor-like [Momordica charantia])

HSP 1 Score: 328.9 bits (842), Expect = 2.6e-86
Identity = 176/176 (100.00%), Postives = 176/176 (100.00%), Query Frame = 0

Query: 1   MANCSVVASSLLAFVSSLVLLLHVASANDVASTICPKTKNPGFCTTELKTAGSADLKALA 60
           MANCSVVASSLLAFVSSLVLLLHVASANDVASTICPKTKNPGFCTTELKTAGSADLKALA
Sbjct: 1   MANCSVVASSLLAFVSSLVLLLHVASANDVASTICPKTKNPGFCTTELKTAGSADLKALA 60

Query: 61  THTLNLARKKAGESVALANDLAAKATDPQLKERYTSCSKNFDEAIGNIETAEKHLASGDY 120
           THTLNLARKKAGESVALANDLAAKATDPQLKERYTSCSKNFDEAIGNIETAEKHLASGDY
Sbjct: 61  THTLNLARKKAGESVALANDLAAKATDPQLKERYTSCSKNFDEAIGNIETAEKHLASGDY 120

Query: 121 NGVNLAGSAVMSTVDDCRANFETGPADTSSLSTNGKTLDDISSIILVISNLLLQPA 177
           NGVNLAGSAVMSTVDDCRANFETGPADTSSLSTNGKTLDDISSIILVISNLLLQPA
Sbjct: 121 NGVNLAGSAVMSTVDDCRANFETGPADTSSLSTNGKTLDDISSIILVISNLLLQPA 176

BLAST of MS019345 vs. NCBI nr
Match: XP_022138711.1 (pectinesterase inhibitor-like [Momordica charantia])

HSP 1 Score: 325.1 bits (832), Expect = 3.7e-85
Identity = 175/176 (99.43%), Postives = 175/176 (99.43%), Query Frame = 0

Query: 1   MANCSVVASSLLAFVSSLVLLLHVASANDVASTICPKTKNPGFCTTELKTAGSADLKALA 60
           MAN SVVASSLLAFVSSLVLLLHVASANDVASTICPKTKNPGFCTTELKTAGSADLKALA
Sbjct: 1   MANSSVVASSLLAFVSSLVLLLHVASANDVASTICPKTKNPGFCTTELKTAGSADLKALA 60

Query: 61  THTLNLARKKAGESVALANDLAAKATDPQLKERYTSCSKNFDEAIGNIETAEKHLASGDY 120
           THTLNLARKKAGESVALANDLAAKATDPQLKERYTSCSKNFDEAIGNIETAEKHLASGDY
Sbjct: 61  THTLNLARKKAGESVALANDLAAKATDPQLKERYTSCSKNFDEAIGNIETAEKHLASGDY 120

Query: 121 NGVNLAGSAVMSTVDDCRANFETGPADTSSLSTNGKTLDDISSIILVISNLLLQPA 177
           NGVNLAGSAVMSTVDDCRANFETGPADTSSLSTNGKTLDDISSIILVISNLLLQPA
Sbjct: 121 NGVNLAGSAVMSTVDDCRANFETGPADTSSLSTNGKTLDDISSIILVISNLLLQPA 176

BLAST of MS019345 vs. NCBI nr
Match: XP_038907188.1 (pectinesterase inhibitor-like [Benincasa hispida])

HSP 1 Score: 181.0 bits (458), Expect = 8.7e-42
Identity = 99/172 (57.56%), Postives = 130/172 (75.58%), Query Frame = 0

Query: 1   MANCSVVASSLLAFVSSLVLLLHVASANDVASTICPKTKNPGFCTTELKTAGSADLKALA 60
           MAN +++ SSLLA    L  ++  A++NDV STICPKT NP FC + LK+AG+ D+K LA
Sbjct: 1   MANSNLILSSLLA----LFTVVSHAASNDVVSTICPKTMNPPFCASVLKSAGTTDMKRLA 60

Query: 61  THTLNLARKKAGESVALANDLAAKATDPQLKERYTSCSKNFDEAIGNIETAEKHLASGDY 120
            +TL LA   A + VALA  LAA AT+ QLK+RY+SCS++FDEA+G+IETA++ LA+GD+
Sbjct: 61  VYTLKLAHTNAAKCVALAKSLAATATNTQLKQRYSSCSESFDEAVGDIETAQEDLAAGDF 120

Query: 121 NGVNLAGSAVMSTVDDCRANFETGPADTSSLSTNGKTLDDISSIILVISNLL 173
           N VN+A S  M+ +DDC+ NF+  P DTS L  NG+TL+DI SIILVISNLL
Sbjct: 121 NAVNIATSGAMTEIDDCQDNFKQPPTDTSMLLKNGRTLNDICSIILVISNLL 168

BLAST of MS019345 vs. NCBI nr
Match: KAE8637390.1 (hypothetical protein CSA_018333, partial [Cucumis sativus])

HSP 1 Score: 177.9 bits (450), Expect = 7.4e-41
Identity = 98/172 (56.98%), Postives = 126/172 (73.26%), Query Frame = 0

Query: 1   MANCSVVASSLLAFVSSLVLLLHVASANDVASTICPKTKNPGFCTTELKTAGSADLKALA 60
           MAN S +    L  V S  ++ +VAS+NDV STICPKT NP FC++ LK+AG+ +LK LA
Sbjct: 18  MANNSCLIIISLVGVLSFTIISNVASSNDVVSTICPKTSNPQFCSSVLKSAGTTNLKGLA 77

Query: 61  THTLNLARKKAGESVALANDLAAKATDPQLKERYTSCSKNFDEAIGNIETAEKHLASGDY 120
            +TLNLA   A +S+ LAN LA  AT+PQLK+RY+SC++++DEAIG+IE A+K LA GD+
Sbjct: 78  VYTLNLAHTNAEKSLTLANSLAKTATNPQLKQRYSSCAESYDEAIGDIENAQKDLALGDF 137

Query: 121 NGVNLAGSAVMSTVDDCRANFETGPADTSSLSTNGKTLDDISSIILVISNLL 173
           NGVN+  S  M+ + DC+  F   P DTS L  NGKTL+DI SIILVISNLL
Sbjct: 138 NGVNIVTSGAMTNIGDCQDKFAQPPKDTSLLLKNGKTLNDICSIILVISNLL 189

BLAST of MS019345 vs. NCBI nr
Match: XP_031740455.1 (pectinesterase inhibitor-like [Cucumis sativus] >XP_031742875.1 pectinesterase inhibitor-like [Cucumis sativus] >XP_031745315.1 pectinesterase inhibitor-like [Cucumis sativus] >XP_031745337.1 pectinesterase inhibitor-like [Cucumis sativus] >XP_031745394.1 pectinesterase inhibitor-like [Cucumis sativus] >XP_031745788.1 pectinesterase inhibitor-like [Cucumis sativus] >XP_031745822.1 pectinesterase inhibitor-like [Cucumis sativus] >XP_031745833.1 pectinesterase inhibitor-like [Cucumis sativus] >XP_031745889.1 pectinesterase inhibitor-like [Cucumis sativus])

HSP 1 Score: 177.9 bits (450), Expect = 7.4e-41
Identity = 98/172 (56.98%), Postives = 126/172 (73.26%), Query Frame = 0

Query: 1   MANCSVVASSLLAFVSSLVLLLHVASANDVASTICPKTKNPGFCTTELKTAGSADLKALA 60
           MAN S +    L  V S  ++ +VAS+NDV STICPKT NP FC++ LK+AG+ +LK LA
Sbjct: 1   MANNSCLIIISLVGVLSFTIISNVASSNDVVSTICPKTSNPQFCSSVLKSAGTTNLKGLA 60

Query: 61  THTLNLARKKAGESVALANDLAAKATDPQLKERYTSCSKNFDEAIGNIETAEKHLASGDY 120
            +TLNLA   A +S+ LAN LA  AT+PQLK+RY+SC++++DEAIG+IE A+K LA GD+
Sbjct: 61  VYTLNLAHTNAEKSLTLANSLAKTATNPQLKQRYSSCAESYDEAIGDIENAQKDLALGDF 120

Query: 121 NGVNLAGSAVMSTVDDCRANFETGPADTSSLSTNGKTLDDISSIILVISNLL 173
           NGVN+  S  M+ + DC+  F   P DTS L  NGKTL+DI SIILVISNLL
Sbjct: 121 NGVNIVTSGAMTNIGDCQDKFAQPPKDTSLLLKNGKTLNDICSIILVISNLL 172

BLAST of MS019345 vs. ExPASy Swiss-Prot
Match: P83326 (Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2)

HSP 1 Score: 101.7 bits (252), Expect = 8.8e-21
Identity = 61/165 (36.97%), Postives = 95/165 (57.58%), Query Frame = 0

Query: 11  LLAFVSSLVLLLHVASANDVASTICPKTKNPGFCTTELKT---AGSADLKALATHTLNLA 70
           ++ F+S L     V + N + S ICPKT+NP  C   L++   + S DLK L   ++++A
Sbjct: 17  VILFISPLSQRPSVKAENHLISEICPKTRNPSLCLQALESDPRSASKDLKGLGQFSIDIA 76

Query: 71  RKKAGESVALANDLAAKATDPQLKERYTSCSKNFDEAIGNIETAEKHLASGDYNGVNLAG 130
           +  A ++  +   L  +ATDP+LK RY +CS+N+ +AI ++  A++ L SGDYN +N+  
Sbjct: 77  QASAKQTSKIIASLTNQATDPKLKGRYETCSENYADAIDSLGQAKQFLTSGDYNSLNIYA 136

Query: 131 SAVMSTVDDCRANFETGPADTSSLSTNGKTLDDISSIILVISNLL 173
           SA       C  +FE  P   + L      L+D+  I+LVISNLL
Sbjct: 137 SAAFDGAGTCEDSFEGPPNIPTQLHQADLKLEDLCDIVLVISNLL 181

BLAST of MS019345 vs. ExPASy Swiss-Prot
Match: Q9LNF2 (Pectinesterase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PMEI1 PE=1 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 8.3e-19
Identity = 64/165 (38.79%), Postives = 94/165 (56.97%), Query Frame = 0

Query: 13  AFVSSLVLLLHVASANDVA----STICPKTKNPGFCTTELKTA-GSADLKALATHTLNLA 72
           AF+SSL+ LL + S+  +     STIC KT NP FC   L T   S +L+ALA  TL+  
Sbjct: 9   AFLSSLMFLLLIGSSYAITSSEMSTICDKTLNPSFCLKFLNTKFASPNLQALAKTTLDST 68

Query: 73  RKKAGESVALANDLAAKATDPQLKERYTSCSKNFDEAIGNIETAEKHLASGDYNGVNLAG 132
           + +A +++     +     DP+ K  Y SC   ++ AIGN+E A +HLASGD  G+N+  
Sbjct: 69  QARATQTLKKLQSIIDGGVDPRSKLAYRSCVDEYESAIGNLEEAFEHLASGDGMGMNMKV 128

Query: 133 SAVMSTVDDCRANFETGPADTSSLSTNGKTLDDISSIILVISNLL 173
           SA +   D C  + +   +  SS+  N KT+ ++  I LVISN+L
Sbjct: 129 SAALDGADTCLDDVKRLRSVDSSVVNNSKTIKNLCGIALVISNML 173

BLAST of MS019345 vs. ExPASy Swiss-Prot
Match: Q9LUV1 (Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 5.6e-15
Identity = 60/170 (35.29%), Postives = 88/170 (51.76%), Query Frame = 0

Query: 6   VVASSLLAFVSSLVLLLHVASANDVASTICPKTKNPGFCTTELKT---AGSADLKALATH 65
           V+ SSL+ FV +  L   VA        IC K KN  FCT+ +K+      ADL+ LA  
Sbjct: 9   VLMSSLMFFVMTGSLNAQVAD----IKAICGKAKNQSFCTSYMKSNPKTSGADLQTLANI 68

Query: 66  TLNLARKKAGESVALANDLAAKATDPQLKERYTSCSKNFDEAIGNIETAEKHLASGDYNG 125
           T   A+  A E       L   AT+P +K+ YTSC +++  AI ++  A++ LASGD  G
Sbjct: 69  TFGSAQTSASEGFRKIQSLVKTATNPTMKKAYTSCVQHYKSAISSLNDAKQSLASGDGKG 128

Query: 126 VNLAGSAVMSTVDDCRANFETGPADTSSLSTNGKTLDDISSIILVISNLL 173
           +N+  SA M     C  +      D S++  +G    +I  I+LVISN++
Sbjct: 129 LNIKVSAAMEGPSTCEQDMADFKVDPSAVKNSG-DFQNICGIVLVISNMM 173

BLAST of MS019345 vs. ExPASy TrEMBL
Match: A0A6J1CA68 (pectinesterase inhibitor-like OS=Momordica charantia OX=3673 GN=LOC111009723 PE=4 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 1.3e-86
Identity = 176/176 (100.00%), Postives = 176/176 (100.00%), Query Frame = 0

Query: 1   MANCSVVASSLLAFVSSLVLLLHVASANDVASTICPKTKNPGFCTTELKTAGSADLKALA 60
           MANCSVVASSLLAFVSSLVLLLHVASANDVASTICPKTKNPGFCTTELKTAGSADLKALA
Sbjct: 1   MANCSVVASSLLAFVSSLVLLLHVASANDVASTICPKTKNPGFCTTELKTAGSADLKALA 60

Query: 61  THTLNLARKKAGESVALANDLAAKATDPQLKERYTSCSKNFDEAIGNIETAEKHLASGDY 120
           THTLNLARKKAGESVALANDLAAKATDPQLKERYTSCSKNFDEAIGNIETAEKHLASGDY
Sbjct: 61  THTLNLARKKAGESVALANDLAAKATDPQLKERYTSCSKNFDEAIGNIETAEKHLASGDY 120

Query: 121 NGVNLAGSAVMSTVDDCRANFETGPADTSSLSTNGKTLDDISSIILVISNLLLQPA 177
           NGVNLAGSAVMSTVDDCRANFETGPADTSSLSTNGKTLDDISSIILVISNLLLQPA
Sbjct: 121 NGVNLAGSAVMSTVDDCRANFETGPADTSSLSTNGKTLDDISSIILVISNLLLQPA 176

BLAST of MS019345 vs. ExPASy TrEMBL
Match: A0A6J1CAH7 (pectinesterase inhibitor-like OS=Momordica charantia OX=3673 GN=LOC111009811 PE=4 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 1.8e-85
Identity = 175/176 (99.43%), Postives = 175/176 (99.43%), Query Frame = 0

Query: 1   MANCSVVASSLLAFVSSLVLLLHVASANDVASTICPKTKNPGFCTTELKTAGSADLKALA 60
           MAN SVVASSLLAFVSSLVLLLHVASANDVASTICPKTKNPGFCTTELKTAGSADLKALA
Sbjct: 1   MANSSVVASSLLAFVSSLVLLLHVASANDVASTICPKTKNPGFCTTELKTAGSADLKALA 60

Query: 61  THTLNLARKKAGESVALANDLAAKATDPQLKERYTSCSKNFDEAIGNIETAEKHLASGDY 120
           THTLNLARKKAGESVALANDLAAKATDPQLKERYTSCSKNFDEAIGNIETAEKHLASGDY
Sbjct: 61  THTLNLARKKAGESVALANDLAAKATDPQLKERYTSCSKNFDEAIGNIETAEKHLASGDY 120

Query: 121 NGVNLAGSAVMSTVDDCRANFETGPADTSSLSTNGKTLDDISSIILVISNLLLQPA 177
           NGVNLAGSAVMSTVDDCRANFETGPADTSSLSTNGKTLDDISSIILVISNLLLQPA
Sbjct: 121 NGVNLAGSAVMSTVDDCRANFETGPADTSSLSTNGKTLDDISSIILVISNLLLQPA 176

BLAST of MS019345 vs. ExPASy TrEMBL
Match: A0A6J1JS95 (pectinesterase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111487936 PE=4 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 4.0e-40
Identity = 96/173 (55.49%), Postives = 125/173 (72.25%), Query Frame = 0

Query: 1   MANCSVVASSLLAFVSSLVLLLHVASANDVASTICPKTKNPGFCTTELKTAGSADLKALA 60
           MAN  V+  S L  +S +    + AS NDV STICPKT+NP FC+  LK+AG++DLK LA
Sbjct: 1   MANSIVLVVSSLFVLSVVFSNGYAASPNDVVSTICPKTRNPSFCSNVLKSAGTSDLKGLA 60

Query: 61  THTLNLARKKAGESVALANDLAAKATDPQLKERYTSCSKNFDEAIGNIETAEKHLASGDY 120
           T TLNLA  KA +S ALA  LA+KATDP+LKE Y +C + FD+A+ +IE A+ +L +GDY
Sbjct: 61  TFTLNLAHDKATDSRALAQSLASKATDPKLKEHYDTCGERFDDAVDDIEGAKSNLGNGDY 120

Query: 121 NGVNLAGSAVMSTVDDCRANFETGPADTSSLSTNGKTLDDISSIILVISNLLL 174
           NGVN + SA M+  DDC  +    P D+S+LS NGKT++DI SIILV+SNLL+
Sbjct: 121 NGVNTSASAAMTEADDCLDSLTKPPKDSSALSDNGKTVEDICSIILVVSNLLI 173

BLAST of MS019345 vs. ExPASy TrEMBL
Match: A0A5D3CV87 (Pectinesterase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold571G00850 PE=4 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 5.2e-40
Identity = 90/162 (55.56%), Postives = 120/162 (74.07%), Query Frame = 0

Query: 11  LLAFVSSLVLLLHVASANDVASTICPKTKNPGFCTTELKTAGSADLKALATHTLNLARKK 70
           +++ +  L+  ++VAS+ DV STICPKT NP FC++ LK+AG+ DLK LA +TLNLA   
Sbjct: 17  IVSLIGVLLFTINVASSTDVVSTICPKTSNPQFCSSVLKSAGTTDLKGLAVYTLNLAHTN 76

Query: 71  AGESVALANDLAAKATDPQLKERYTSCSKNFDEAIGNIETAEKHLASGDYNGVNLAGSAV 130
           A +S+ LAN LA   T+PQLK+RY+SC +++DEA+G+IE  +K LA GD+NGVN+  S  
Sbjct: 77  ARKSLTLANSLAKTTTNPQLKQRYSSCVESYDEAVGDIENVQKDLALGDFNGVNIVTSGA 136

Query: 131 MSTVDDCRANFETGPADTSSLSTNGKTLDDISSIILVISNLL 173
           M+ +DDC+  F   P DTS L  NGKTL+DI SIILVISNLL
Sbjct: 137 MTEIDDCQDKFAQPPKDTSLLLKNGKTLNDICSIILVISNLL 178

BLAST of MS019345 vs. ExPASy TrEMBL
Match: A0A5A7V7V7 (Pectinesterase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold89G00500 PE=4 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 5.2e-40
Identity = 90/162 (55.56%), Postives = 120/162 (74.07%), Query Frame = 0

Query: 11  LLAFVSSLVLLLHVASANDVASTICPKTKNPGFCTTELKTAGSADLKALATHTLNLARKK 70
           +++ +  L+  ++VAS+ DV STICPKT NP FC++ LK+AG+ DLK LA +TLNLA   
Sbjct: 9   IVSLIGVLLFTINVASSTDVVSTICPKTSNPQFCSSVLKSAGTTDLKGLAVYTLNLAHTN 68

Query: 71  AGESVALANDLAAKATDPQLKERYTSCSKNFDEAIGNIETAEKHLASGDYNGVNLAGSAV 130
           A +S+ LAN LA   T+PQLK+RY+SC +++DEA+G+IE  +K LA GD+NGVN+  S  
Sbjct: 69  ARKSLTLANSLAKTTTNPQLKQRYSSCVESYDEAVGDIENVQKDLALGDFNGVNIVTSGA 128

Query: 131 MSTVDDCRANFETGPADTSSLSTNGKTLDDISSIILVISNLL 173
           M+ +DDC+  F   P DTS L  NGKTL+DI SIILVISNLL
Sbjct: 129 MTEIDDCQDKFAQPPKDTSLLLKNGKTLNDICSIILVISNLL 170

BLAST of MS019345 vs. TAIR 10
Match: AT1G48020.1 (pectin methylesterase inhibitor 1 )

HSP 1 Score: 95.1 bits (235), Expect = 5.9e-20
Identity = 64/165 (38.79%), Postives = 94/165 (56.97%), Query Frame = 0

Query: 13  AFVSSLVLLLHVASANDVA----STICPKTKNPGFCTTELKTA-GSADLKALATHTLNLA 72
           AF+SSL+ LL + S+  +     STIC KT NP FC   L T   S +L+ALA  TL+  
Sbjct: 9   AFLSSLMFLLLIGSSYAITSSEMSTICDKTLNPSFCLKFLNTKFASPNLQALAKTTLDST 68

Query: 73  RKKAGESVALANDLAAKATDPQLKERYTSCSKNFDEAIGNIETAEKHLASGDYNGVNLAG 132
           + +A +++     +     DP+ K  Y SC   ++ AIGN+E A +HLASGD  G+N+  
Sbjct: 69  QARATQTLKKLQSIIDGGVDPRSKLAYRSCVDEYESAIGNLEEAFEHLASGDGMGMNMKV 128

Query: 133 SAVMSTVDDCRANFETGPADTSSLSTNGKTLDDISSIILVISNLL 173
           SA +   D C  + +   +  SS+  N KT+ ++  I LVISN+L
Sbjct: 129 SAALDGADTCLDDVKRLRSVDSSVVNNSKTIKNLCGIALVISNML 173

BLAST of MS019345 vs. TAIR 10
Match: AT3G17220.1 (pectin methylesterase inhibitor 2 )

HSP 1 Score: 82.4 bits (202), Expect = 3.9e-16
Identity = 60/170 (35.29%), Postives = 88/170 (51.76%), Query Frame = 0

Query: 6   VVASSLLAFVSSLVLLLHVASANDVASTICPKTKNPGFCTTELKT---AGSADLKALATH 65
           V+ SSL+ FV +  L   VA        IC K KN  FCT+ +K+      ADL+ LA  
Sbjct: 9   VLMSSLMFFVMTGSLNAQVAD----IKAICGKAKNQSFCTSYMKSNPKTSGADLQTLANI 68

Query: 66  TLNLARKKAGESVALANDLAAKATDPQLKERYTSCSKNFDEAIGNIETAEKHLASGDYNG 125
           T   A+  A E       L   AT+P +K+ YTSC +++  AI ++  A++ LASGD  G
Sbjct: 69  TFGSAQTSASEGFRKIQSLVKTATNPTMKKAYTSCVQHYKSAISSLNDAKQSLASGDGKG 128

Query: 126 VNLAGSAVMSTVDDCRANFETGPADTSSLSTNGKTLDDISSIILVISNLL 173
           +N+  SA M     C  +      D S++  +G    +I  I+LVISN++
Sbjct: 129 LNIKVSAAMEGPSTCEQDMADFKVDPSAVKNSG-DFQNICGIVLVISNMM 173

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022138606.12.6e-86100.00pectinesterase inhibitor-like [Momordica charantia][more]
XP_022138711.13.7e-8599.43pectinesterase inhibitor-like [Momordica charantia][more]
XP_038907188.18.7e-4257.56pectinesterase inhibitor-like [Benincasa hispida][more]
KAE8637390.17.4e-4156.98hypothetical protein CSA_018333, partial [Cucumis sativus][more]
XP_031740455.17.4e-4156.98pectinesterase inhibitor-like [Cucumis sativus] >XP_031742875.1 pectinesterase i... [more]
Match NameE-valueIdentityDescription
P833268.8e-2136.97Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2[more]
Q9LNF28.3e-1938.79Pectinesterase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PMEI1 PE=1 SV=1[more]
Q9LUV15.6e-1535.29Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CA681.3e-86100.00pectinesterase inhibitor-like OS=Momordica charantia OX=3673 GN=LOC111009723 PE=... [more]
A0A6J1CAH71.8e-8599.43pectinesterase inhibitor-like OS=Momordica charantia OX=3673 GN=LOC111009811 PE=... [more]
A0A6J1JS954.0e-4055.49pectinesterase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111487936 PE=4 S... [more]
A0A5D3CV875.2e-4055.56Pectinesterase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A5A7V7V75.2e-4055.56Pectinesterase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc... [more]
Match NameE-valueIdentityDescription
AT1G48020.15.9e-2038.79pectin methylesterase inhibitor 1 [more]
AT3G17220.13.9e-1635.29pectin methylesterase inhibitor 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 26..168
e-value: 3.4E-15
score: 66.5
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 32..167
e-value: 3.6E-13
score: 50.1
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 9..172
e-value: 5.3E-24
score: 82.5
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 26..173
e-value: 7.0E-28
score: 99.5
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 32..172
NoneNo IPR availablePANTHERPTHR36710PECTINESTERASE INHIBITOR-LIKEcoord: 6..172
NoneNo IPR availablePANTHERPTHR36710:SF6PECTINESTERASE INHIBITOR-LIKEcoord: 6..172
IPR034086Pectinesterase inhibitor, plantCDDcd15797PMEIcoord: 32..172
e-value: 4.52721E-39
score: 128.694

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS019345.1MS019345.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0046910 pectinesterase inhibitor activity