MS019325 (gene) Bitter gourd (TR) v1

Overview


Name	MS019325
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	AMSH-like ubiquitin thioesterase 3
Location	scaffold611: 380089 .. 384499 (-)
RNA-Seq Expression	MS019325
Synteny	MS019325

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGATCAACATCGATAGGGAGGCGCGAAAAGTTACGGTTGATAGTCGAATTCCCCTCCGGAATTACTATCGGATCGCTGATAATCTATTAAAACAGGTGATCTTTCCGGAACCCTTCTTCTCGATTGTTCCATTCTCACCATTTTAGTTTTTTTTTTTTTTTGGTCCTTTGTTCTGACTGATTTTTTTATGCTGAATTGGTAATGGTTTATTAGAAATTCTGCCAATCTCTATTTATTTCTCTACATTTTTGGTTATTGAAGTGATTTCGACGGTTCATAACTTGTCGAATGGAAATTTTCTATAATGGCGATTTGAGTATAGGAGTGCGTATAGGTTGCGATGGAACCATTTTTTTTTATGTTATTAAGTTGCTTCCTTAAATTGGCAGGCTAGTATCTATCGGGAGGAGAATAATGTCGTGGATTTGTTCATCATACTTCTTAGGTTTTCAAGGTAAGGGACATGTTGATACTTGTATTTCCATGGCCAATGGATGTCGATTGTTAGTGTGGGAAACCCACTCTTTAACTTGTTATTATTTTCCCAGCTTGGTTTCTGAAACTATACCACGGCATCGAGATTACCAGATATTTTTCCCAAAGGAAAAGATATTTTATAAGAAGGTGAGTTAGAATTCAACATCTCTTTCTCCTTTTTGTGCACTGTGTATGAATGTTGTGTCATTTCATCTTCATTTTTTCCTATCAGAAACTGTTAAATGTAGTAGATGAACTCGAGTCCTTGAAGCCCGAATTTGATTGCCGAGTAAATGAACTGGAGAAAGTGCATGCACAACCTCGGCTTCCTCCTCCAGATGATCAGGACAGAAGCTCATATACTCTCGGGCCATCTTCCATGGAATGGCCCTCTGTGAATAGAGATTATTCATTGGTTTTGGACAGAAAGCAGGTATCGACTCAAAATGTTTGAATGATACTTTCTTCGTTTGTAACTATTTGACCCATCAATGGCAATTCCACTAATTATTTGGAATTTTATTTTACTTCTAGTCTTTTGCAGTTTCTATTTTTTGCATTTTCATCTATGAATACGTGATTATTGTTACCATGAACATGTGATTACTGTTATGTTATTCTTAATTTGATTTCACTCTGTTAATCCTCACCCTCCCCTCTCCTCATTATTCTCAGCATGCCAGTTTACAGCCACAATCTTCATGGAGGTATAACCATGACCAGGGTCAAGTTTCACAATCAAGCTCCTTTAAGATTGCGAATAAGTTCCCAAACCTGTGAGTATACTTTTTGTACATGTTTATTATGATCCATTAGCGATGCAACAGTTCACCCCTCTGGGTGGAAAATAACCTTTTGATGATGTATGGGGGTAAATCACCCTAAAACTTTTATTCTAGTGTACACCAGAAGAAAGTGATTTTTTAGTTCAAGTAGCTGGTCAGTGGTCAGGGAGTAGAAGACAAATTGTAATGACTACATCTTGCATTCTGAATATAGCTGACTGAAAAAGACAAAGTATATTGTTGATGTTGAGTTCTTCTGCAACTGGTCTCTAGATTGCATAAAAAGATAGTGTCTTTTGCACAAGGGCTATATATTGTCATATCTTGAATGCCTGTGTCTTCTTCATATGATGCTCCACTTGGATCCTATGGAAGCCATAAGAATACAATTCTAGGCATACCTATACAGTCACCACTTTTCATAAAACATTCACGAAGAATCTGATAATTAGCACTGGGAACGTGCAGGTCTCTTAATGTACCACTTCCGAAACCCGAAACTTTGTCTCGACATTCTATTTTGGGACCCAGTGGTCTTCAGGGCCAATGGAAGGGACCGAGCACAGATAAGGTGAATTTTGCACATTCTCTTATATGTTGTTTCCTGTTGTTTTTGCTTCCATTAACAGGTTAATTCTATGGTTCTGTACAGATTCACTATCCAAGAAATCAGGACTTCACTCCTGAAGATCCAAGGTTAGAGGCGTGTATAACTTGTATTAGTATCTTAAAGTTTACACATATTTTCTCATAAAAAAAGTTGCATATCTTTTGTCATCTGTGTTGAATTTTTACCTTCCTGATGACAAACACTTAATGAGACTTGAGAGAAATATTTGATTTCATTATTTTACTTTTTATCCGATCATGTTTTCTTGAAACATCTATTTGGAACCTTCAAATCCTTACAGACTGAATCAAATTGAACAATGTGATCTAGTGGCACATAAAGATAACGATTCAGGAGATCTTATGTCGATGATGGAGTCGGTGCTTTCACTGGATGATGGTAGATGGCCACATCCTGCAGACATGTCTACTCCTCCGTTGATAACAGAAGTAAGGGAGGATCCTTTCCCATTGGTCAAACAACCTTCTCCTCCTCCTGTTCTTGCTAAGGTTCAGCAGGAGTATGCGACAATTCCCCCATCTAAAGTTGCGGATCCAAGGCCTGGAATGGCAAAACAATCGCAGGATGGGCCTGACTCGTTTCAACGTTTACAAATTGTAGGCCTTTTCACTTTGATTCTCAGTAGATAATTTCAGAAAGCAATTATTTGTTTGCTAATTTCTTTTGTCATGTCCTACCCTTGCAAAATAATGTGTACACCTTGCAATATGATATTATTACTTCTCCTTTATGCTGCAGCCAGTAAAAATGCTGGATGATTTCTTGAGAGTGGCTCGAGAAAATACAAATAAAAACCTGGAGACTTGTGGTGTTTTAGCTGGCTCGCTGGTAAGAGCAAATTAATGATTTAATGTAAAAAATTTAAGATTTTCTAGTTGGCAAGTATCATTCTCATACTTCATCATTAAATTTTTGCAGAAAAACAGGGTTTTTTACATATCTACGCTCATAATCCCAAAGCAAGAATCAACATCAGATTCGGTAAGAATATGATTACTTTTTTTTTCCAAGGTTAAATTAGCCATTTCATCATCCACATTATTGTTTTTACTACTCTTGAATGTTTGGTTCCCATTTGACCGGTGATTATACTAGATTTAAACATGTAACGTGGAAGTAGGTTGTACGTATGTTTGAGTTCTAGTTGTTTTATATTCCGAGTATTTTTCTTATTACTTTAACGTGCATTTCTATGATACTTGTCCATACAATGGTTATAAATTTATAATTGACTTGAAGATTAGTTGATTGAGCTTAGTTAGTTATCACTTATTTTTATTGCCTTTAATTAATTCTTAGAATCATTTTGTAATGCTCAGTGCCAAACGTTGAATGAAGAGGAAATATTTGAAGTTCAAGACAGATTATCACTTTTCCCTCTTGGTTGGATCCATGTAAGCTTTCTGGCCCTCCATGCAATAGATGATTATGCATGCTGAGTTATTGTATCGTTTTGTATTTTACATTCAGTCTAAAGATAAAACTAAAAGAAAAACTTTTTCTTGATAGGAAGTTATGCTAAGTTATTGTGGAATTTTGTATTTAAATCACTCTTTCAGTGGTCATCTCATAGTTCATCTGATTGAGCCTATCTGGTACCTTGTGTTGTTCCTTGGCTAATACTTTCCAATTCTTACACAAGTTTAAACTTCAAGGAATTTGTAAATAGGGGGACTTAAAATTAATACCATACACTTACTATCCTGAACAGCCTTATCTTTATAAATTAATAAGCTTTGGCATTATTTCAGTTAAAAAGTTGACTTGCTATGCAGTGATATACACATGCTCTTTGTAATGCTGGAAAGTAGAAGAGTGAGATGTAACTTTCAATATAGATGTCCTTGAGCAAATATAAATTTGTGACTTCAACATTTTTGTACGGCACGTACCGTTACTGCAATTCAAGAATCTTTTTTTTTTTTTTTTTTTCCAGTCTCTGATTTTTGTTTGGTTCTCAGACACACCCATCACAGACCTGTTTCATGTCATCTGTGGATCTGCACACTCATTACTCCTACCAGGTAATATAACAAAACTGCTTTAGTTACTTTCATAACATAGAAAGTTGCATCATCATGGAATGAAGGTGCCAATTTGTAAAGATATTAACCTCTACAATTTGTGGTTTTTCCTGTTTGCATTGTTTGTTCTTCCTGTTCTAGTTTTTTTACCCTCTTTTAAGTAGTTTTTGGTCTCTTCAGATTATGTTGCCAGAAGCAATTGCAATCGTGATGGCTCCTACTGATACGTCAAGGTATTCATTCAACTTATTAGTAGTTTCAGTATACCCAATACTCAGATCTTGTATAATTTGCTAATGTGTCCCATTGCCATCGCAGTCCATATGGCATATTTCATCTGTCTGATCCGGGTGGCGTGTCAGTCATCCGCAACTGCCAACAGCGTGGATTTCATCCCCACGAGGAGCCTGAAGATGGGAGTCCACTCTATGAGCACTGCTCTCATGTTTTGATGAATCCCAACTTGAAGTTTGACGTCGTTGATCTGAGG

mRNA sequence

ATGAAGATCAACATCGATAGGGAGGCGCGAAAAGTTACGGTTGATAGTCGAATTCCCCTCCGGAATTACTATCGGATCGCTGATAATCTATTAAAACAGGCTAGTATCTATCGGGAGGAGAATAATGTCGTGGATTTGTTCATCATACTTCTTAGGTTTTCAAGCTTGGTTTCTGAAACTATACCACGGCATCGAGATTACCAGATATTTTTCCCAAAGGAAAAGATATTTTATAAGAAGAAACTGTTAAATGTAGTAGATGAACTCGAGTCCTTGAAGCCCGAATTTGATTGCCGAGTAAATGAACTGGAGAAAGTGCATGCACAACCTCGGCTTCCTCCTCCAGATGATCAGGACAGAAGCTCATATACTCTCGGGCCATCTTCCATGGAATGGCCCTCTGTGAATAGAGATTATTCATTGGTTTTGGACAGAAAGCAGCATGCCAGTTTACAGCCACAATCTTCATGGAGGTATAACCATGACCAGGGTCAAGTTTCACAATCAAGCTCCTTTAAGATTGCGAATAAGTTCCCAAACCTGTCTCTTAATGTACCACTTCCGAAACCCGAAACTTTGTCTCGACATTCTATTTTGGGACCCAGTGGTCTTCAGGGCCAATGGAAGGGACCGAGCACAGATAAGATTCACTATCCAAGAAATCAGGACTTCACTCCTGAAGATCCAAGACTGAATCAAATTGAACAATGTGATCTAGTGGCACATAAAGATAACGATTCAGGAGATCTTATGTCGATGATGGAGTCGGTGCTTTCACTGGATGATGGTAGATGGCCACATCCTGCAGACATGTCTACTCCTCCGTTGATAACAGAAGTAAGGGAGGATCCTTTCCCATTGGTCAAACAACCTTCTCCTCCTCCTGTTCTTGCTAAGGTTCAGCAGGAGTATGCGACAATTCCCCCATCTAAAGTTGCGGATCCAAGGCCTGGAATGGCAAAACAATCGCAGGATGGGCCTGACTCGTTTCAACGTTTACAAATTCCAGTAAAAATGCTGGATGATTTCTTGAGAGTGGCTCGAGAAAATACAAATAAAAACCTGGAGACTTGTGGTGTTTTAGCTGGCTCGCTGAAAAACAGGGTTTTTTACATATCTACGCTCATAATCCCAAAGCAAGAATCAACATCAGATTCGTGCCAAACGTTGAATGAAGAGGAAATATTTGAAGTTCAAGACAGATTATCACTTTTCCCTCTTGGTTGGATCCATACACACCCATCACAGACCTGTTTCATGTCATCTGTGGATCTGCACACTCATTACTCCTACCAGATTATGTTGCCAGAAGCAATTGCAATCGTGATGGCTCCTACTGATACGTCAAGTCCATATGGCATATTTCATCTGTCTGATCCGGGTGGCGTGTCAGTCATCCGCAACTGCCAACAGCGTGGATTTCATCCCCACGAGGAGCCTGAAGATGGGAGTCCACTCTATGAGCACTGCTCTCATGTTTTGATGAATCCCAACTTGAAGTTTGACGTCGTTGATCTGAGG

Coding sequence (CDS)

ATGAAGATCAACATCGATAGGGAGGCGCGAAAAGTTACGGTTGATAGTCGAATTCCCCTCCGGAATTACTATCGGATCGCTGATAATCTATTAAAACAGGCTAGTATCTATCGGGAGGAGAATAATGTCGTGGATTTGTTCATCATACTTCTTAGGTTTTCAAGCTTGGTTTCTGAAACTATACCACGGCATCGAGATTACCAGATATTTTTCCCAAAGGAAAAGATATTTTATAAGAAGAAACTGTTAAATGTAGTAGATGAACTCGAGTCCTTGAAGCCCGAATTTGATTGCCGAGTAAATGAACTGGAGAAAGTGCATGCACAACCTCGGCTTCCTCCTCCAGATGATCAGGACAGAAGCTCATATACTCTCGGGCCATCTTCCATGGAATGGCCCTCTGTGAATAGAGATTATTCATTGGTTTTGGACAGAAAGCAGCATGCCAGTTTACAGCCACAATCTTCATGGAGGTATAACCATGACCAGGGTCAAGTTTCACAATCAAGCTCCTTTAAGATTGCGAATAAGTTCCCAAACCTGTCTCTTAATGTACCACTTCCGAAACCCGAAACTTTGTCTCGACATTCTATTTTGGGACCCAGTGGTCTTCAGGGCCAATGGAAGGGACCGAGCACAGATAAGATTCACTATCCAAGAAATCAGGACTTCACTCCTGAAGATCCAAGACTGAATCAAATTGAACAATGTGATCTAGTGGCACATAAAGATAACGATTCAGGAGATCTTATGTCGATGATGGAGTCGGTGCTTTCACTGGATGATGGTAGATGGCCACATCCTGCAGACATGTCTACTCCTCCGTTGATAACAGAAGTAAGGGAGGATCCTTTCCCATTGGTCAAACAACCTTCTCCTCCTCCTGTTCTTGCTAAGGTTCAGCAGGAGTATGCGACAATTCCCCCATCTAAAGTTGCGGATCCAAGGCCTGGAATGGCAAAACAATCGCAGGATGGGCCTGACTCGTTTCAACGTTTACAAATTCCAGTAAAAATGCTGGATGATTTCTTGAGAGTGGCTCGAGAAAATACAAATAAAAACCTGGAGACTTGTGGTGTTTTAGCTGGCTCGCTGAAAAACAGGGTTTTTTACATATCTACGCTCATAATCCCAAAGCAAGAATCAACATCAGATTCGTGCCAAACGTTGAATGAAGAGGAAATATTTGAAGTTCAAGACAGATTATCACTTTTCCCTCTTGGTTGGATCCATACACACCCATCACAGACCTGTTTCATGTCATCTGTGGATCTGCACACTCATTACTCCTACCAGATTATGTTGCCAGAAGCAATTGCAATCGTGATGGCTCCTACTGATACGTCAAGTCCATATGGCATATTTCATCTGTCTGATCCGGGTGGCGTGTCAGTCATCCGCAACTGCCAACAGCGTGGATTTCATCCCCACGAGGAGCCTGAAGATGGGAGTCCACTCTATGAGCACTGCTCTCATGTTTTGATGAATCCCAACTTGAAGTTTGACGTCGTTGATCTGAGG

Protein sequence

MKINIDREARKVTVDSRIPLRNYYRIADNLLKQASIYREENNVVDLFIILLRFSSLVSETIPRHRDYQIFFPKEKIFYKKKLLNVVDELESLKPEFDCRVNELEKVHAQPRLPPPDDQDRSSYTLGPSSMEWPSVNRDYSLVLDRKQHASLQPQSSWRYNHDQGQVSQSSSFKIANKFPNLSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIHYPRNQDFTPEDPRLNQIEQCDLVAHKDNDSGDLMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGMAKQSQDGPDSFQRLQIPVKMLDDFLRVARENTNKNLETCGVLAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNLKFDVVDLR

Homology

[Close]

BLAST of MS019325 vs. NCBI nr
Match: XP_022138746.1 (AMSH-like ubiquitin thioesterase 3 [Momordica charantia])

HSP 1 Score: 1027.7 bits (2656), Expect = 3.4e-296
Identity = 505/507 (99.61%), Postives = 505/507 (99.61%), Query Frame = 0


Query: 1   MKINIDREARKVTVDSRIPLRNYYRIADNLLKQASIYREENNVVDLFIILLRFSSLVSET 60
           MKINIDREARKVTVDSRIPLRNYYRIADNLLKQASIYREENNVVDLFIILLRFSSLVSET
Sbjct: 1   MKINIDREARKVTVDSRIPLRNYYRIADNLLKQASIYREENNVVDLFIILLRFSSLVSET 60

Query: 61  IPRHRDYQIFFPKEKIFYKKKLLNVVDELESLKPEFDCRVNELEKVHAQPRLPPPDDQDR 120
           IPRHRDYQIFFPKEKIFYKKKLLNVVDELESLKPEFDCRVNELEKVHAQPRLPPPDDQDR
Sbjct: 61  IPRHRDYQIFFPKEKIFYKKKLLNVVDELESLKPEFDCRVNELEKVHAQPRLPPPDDQDR 120

Query: 121 SSYTLGPSSMEWPSVNRDYSLVLDRKQHASLQPQSSWRYNHDQGQVSQSSSFKIANKFPN 180
           SSYTLGPSSMEWPSVNRDYSLVLDRKQHASLQPQSSWRYNHDQGQVSQSSSFKIANKFPN
Sbjct: 121 SSYTLGPSSMEWPSVNRDYSLVLDRKQHASLQPQSSWRYNHDQGQVSQSSSFKIANKFPN 180

Query: 181 LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIHYPRNQDFTPEDPRLNQIEQCDLV 240
           LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIHYPRNQDFTPEDPRLNQIEQCDLV
Sbjct: 181 LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIHYPRNQDFTPEDPRLNQIEQCDLV 240

Query: 241 AHKDNDSGDLMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 300
           AHKDN SG LMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV
Sbjct: 241 AHKDNYSGGLMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 300

Query: 301 QQEYATIPPSKVADPRPGMAKQSQDGPDSFQRLQIPVKMLDDFLRVARENTNKNLETCGV 360
           QQEYATIPPSKVADPRPGMAKQSQDGPDSFQRLQIPVKMLDDFLRVARENTNKNLETCGV
Sbjct: 301 QQEYATIPPSKVADPRPGMAKQSQDGPDSFQRLQIPVKMLDDFLRVARENTNKNLETCGV 360

Query: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420
           LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM
Sbjct: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420

Query: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480
           SSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP
Sbjct: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480

Query: 481 EDGSPLYEHCSHVLMNPNLKFDVVDLR 508
           EDGSPLYEHCSHVLMNPNLKFDVVDLR
Sbjct: 481 EDGSPLYEHCSHVLMNPNLKFDVVDLR 507

[Close]

BLAST of MS019325 vs. NCBI nr
Match: XP_038875747.1 (AMSH-like ubiquitin thioesterase 3 [Benincasa hispida])

HSP 1 Score: 917.5 bits (2370), Expect = 4.9e-263
Identity = 452/507 (89.15%), Postives = 474/507 (93.49%), Query Frame = 0


Query: 1   MKINIDREARKVTVDSRIPLRNYYRIADNLLKQASIYREENNVVDLFIILLRFSSLVSET 60
           MKINIDREARKV VD+RIPLRNY+RIADNLLKQA IYREENNVVDLFIILLRFSSLVSET
Sbjct: 1   MKINIDREARKVAVDNRIPLRNYFRIADNLLKQADIYREENNVVDLFIILLRFSSLVSET 60

Query: 61  IPRHRDYQIFFPKEKIFYKKKLLNVVDELESLKPEFDCRVNELEKVHAQPRLPPPDDQDR 120
           IPRHRDYQ FFPKEKIFYKKKLL+VVDELESLKPEFD RVNELEKV+ +PRLPPPD+Q  
Sbjct: 61  IPRHRDYQAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRPRLPPPDEQGS 120

Query: 121 SSYTLGPSSMEWPSVNRDYSLVLDRKQHASLQPQSSWRYNHDQGQVSQSSSFKIANKFPN 180
            S +L PSSME P  N +YSLVLD+KQH++ +PQSSWRY  D+G VSQS+S +I NKF +
Sbjct: 121 VSNSLRPSSMEQPYANGNYSLVLDQKQHSNSRPQSSWRYGDDRGHVSQSNSLRIENKFSS 180

Query: 181 LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIHYPRNQDFTPEDPRLNQIEQCDLV 240
           LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKI YPRNQDFTPED RLNQIEQCDLV
Sbjct: 181 LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIQYPRNQDFTPEDSRLNQIEQCDLV 240

Query: 241 AHKDNDSGDLMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 300
           AHKD+  G +MSMMESVLSLDDGRWPHPADMSTPPLITE REDPFPLVKQPSPPPVLAKV
Sbjct: 241 AHKDSGPGGVMSMMESVLSLDDGRWPHPADMSTPPLITEAREDPFPLVKQPSPPPVLAKV 300

Query: 301 QQEYATIPPSKVADPRPGMAKQSQDGPDSFQRLQIPVKMLDDFLRVARENTNKNLETCGV 360
           QQEYATIPPSKVADPRPG+AKQS D  +SFQRL IPVKMLDDFLR+ARENTNKNLETCGV
Sbjct: 301 QQEYATIPPSKVADPRPGVAKQSLDESESFQRLHIPVKMLDDFLRLARENTNKNLETCGV 360

Query: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420
           LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM
Sbjct: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420

Query: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480
           SSVDLHTHYSYQIMLPEAIAIVMAPTD SSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP
Sbjct: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480

Query: 481 EDGSPLYEHCSHVLMNPNLKFDVVDLR 508
           EDGSPLYEHCSHVLMNPN KFDV+DLR
Sbjct: 481 EDGSPLYEHCSHVLMNPNAKFDVIDLR 507

[Close]

BLAST of MS019325 vs. NCBI nr
Match: TYK02941.1 (AMSH-like ubiquitin thioesterase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 902.5 bits (2331), Expect = 1.6e-258
Identity = 443/507 (87.38%), Postives = 472/507 (93.10%), Query Frame = 0


Query: 1   MKINIDREARKVTVDSRIPLRNYYRIADNLLKQASIYREENNVVDLFIILLRFSSLVSET 60
           MKINIDREARKV VD+RIPLRNY+RIADNLLKQA+IYREENNVVDLFIILLRFSSLVSET
Sbjct: 54  MKINIDREARKVAVDNRIPLRNYFRIADNLLKQANIYREENNVVDLFIILLRFSSLVSET 113

Query: 61  IPRHRDYQIFFPKEKIFYKKKLLNVVDELESLKPEFDCRVNELEKVHAQPRLPPPDDQDR 120
           IPRHRDYQ FFPKEKI+YKKKLLNVVDELESLKPEFD RVNELEKV+ +PRLP PD+Q  
Sbjct: 114 IPRHRDYQAFFPKEKIYYKKKLLNVVDELESLKPEFDRRVNELEKVYGRPRLPLPDEQGS 173

Query: 121 SSYTLGPSSMEWPSVNRDYSLVLDRKQHASLQPQSSWRYNHDQGQVSQSSSFKIANKFPN 180
            S +L PS  E P  N +YSL+LD+KQH + +PQSSWRY+ D+GQVSQSSS ++ NKF  
Sbjct: 174 VSNSLRPSPKEQPYANGNYSLMLDQKQHVNSRPQSSWRYSDDRGQVSQSSSLRLENKFST 233

Query: 181 LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIHYPRNQDFTPEDPRLNQIEQCDLV 240
           +SLNVPLPKPETLSRHSILGPSGLQ QW GPSTDKI YPRNQDFTPED RLNQ E CDLV
Sbjct: 234 MSLNVPLPKPETLSRHSILGPSGLQCQWMGPSTDKIQYPRNQDFTPEDSRLNQSEHCDLV 293

Query: 241 AHKDNDSGDLMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 300
           AHKD+ SG +MSMMESVLSLDDGRWP+PADMSTPPLITEVREDPFPLVKQPSPPPVLAKV
Sbjct: 294 AHKDSGSGGVMSMMESVLSLDDGRWPNPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 353

Query: 301 QQEYATIPPSKVADPRPGMAKQSQDGPDSFQRLQIPVKMLDDFLRVARENTNKNLETCGV 360
           QQEYATIPPSKVADPRPG+A+QSQD  +SFQRL IPVKMLDDFLR+ARENTNKNLETCGV
Sbjct: 354 QQEYATIPPSKVADPRPGVARQSQDESESFQRLHIPVKMLDDFLRLARENTNKNLETCGV 413

Query: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420
           LAGSLKNR+FYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM
Sbjct: 414 LAGSLKNRIFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 473

Query: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480
           SSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP
Sbjct: 474 SSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 533

Query: 481 EDGSPLYEHCSHVLMNPNLKFDVVDLR 508
           +DGSPLYEHCSHV+MNPN KFDV+DLR
Sbjct: 534 DDGSPLYEHCSHVMMNPNAKFDVIDLR 560

[Close]

BLAST of MS019325 vs. NCBI nr
Match: XP_008458380.1 (PREDICTED: LOW QUALITY PROTEIN: AMSH-like ubiquitin thioesterase 3 [Cucumis melo])

HSP 1 Score: 902.5 bits (2331), Expect = 1.6e-258
Identity = 443/507 (87.38%), Postives = 472/507 (93.10%), Query Frame = 0


Query: 1   MKINIDREARKVTVDSRIPLRNYYRIADNLLKQASIYREENNVVDLFIILLRFSSLVSET 60
           MKINIDREARKV VD+RIPLRNY+RIADNLLKQA+IYREENNVVDLFIILLRFSSLVSET
Sbjct: 1   MKINIDREARKVAVDNRIPLRNYFRIADNLLKQANIYREENNVVDLFIILLRFSSLVSET 60

Query: 61  IPRHRDYQIFFPKEKIFYKKKLLNVVDELESLKPEFDCRVNELEKVHAQPRLPPPDDQDR 120
           IPRHRDYQ FFPKEKI+YKKKLLNVVDELESLKPEFD RVNELEKV+ +PRLP PD+Q  
Sbjct: 61  IPRHRDYQAFFPKEKIYYKKKLLNVVDELESLKPEFDRRVNELEKVYGRPRLPLPDEQGS 120

Query: 121 SSYTLGPSSMEWPSVNRDYSLVLDRKQHASLQPQSSWRYNHDQGQVSQSSSFKIANKFPN 180
            S +L PS  E P  N +YSL+LD+KQH + +PQSSWRY+ D+GQVSQSSS ++ NKF  
Sbjct: 121 VSNSLRPSPKEQPYANGNYSLMLDQKQHVNSRPQSSWRYSDDRGQVSQSSSLRLENKFST 180

Query: 181 LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIHYPRNQDFTPEDPRLNQIEQCDLV 240
           +SLNVPLPKPETLSRHSILGPSGLQ QW GPSTDKI YPRNQDFTPED RLNQ E CDLV
Sbjct: 181 MSLNVPLPKPETLSRHSILGPSGLQCQWMGPSTDKIQYPRNQDFTPEDSRLNQSEHCDLV 240

Query: 241 AHKDNDSGDLMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 300
           AHKD+ SG +MSMMESVLSLDDGRWP+PADMSTPPLITEVREDPFPLVKQPSPPPVLAKV
Sbjct: 241 AHKDSGSGGVMSMMESVLSLDDGRWPNPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 300

Query: 301 QQEYATIPPSKVADPRPGMAKQSQDGPDSFQRLQIPVKMLDDFLRVARENTNKNLETCGV 360
           QQEYATIPPSKVADPRPG+A+QSQD  +SFQRL IPVKMLDDFLR+ARENTNKNLETCGV
Sbjct: 301 QQEYATIPPSKVADPRPGVARQSQDESESFQRLHIPVKMLDDFLRLARENTNKNLETCGV 360

Query: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420
           LAGSLKNR+FYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM
Sbjct: 361 LAGSLKNRIFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420

Query: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480
           SSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP
Sbjct: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480

Query: 481 EDGSPLYEHCSHVLMNPNLKFDVVDLR 508
           +DGSPLYEHCSHV+MNPN KFDV+DLR
Sbjct: 481 DDGSPLYEHCSHVMMNPNAKFDVIDLR 507

[Close]

BLAST of MS019325 vs. NCBI nr
Match: XP_023006405.1 (AMSH-like ubiquitin thioesterase 3 isoform X1 [Cucurbita maxima])

HSP 1 Score: 901.7 bits (2329), Expect = 2.8e-258
Identity = 448/507 (88.36%), Postives = 468/507 (92.31%), Query Frame = 0


Query: 1   MKINIDREARKVTVDSRIPLRNYYRIADNLLKQASIYREENNVVDLFIILLRFSSLVSET 60
           MKINIDREARKV VD+RIPLRNY+RIADNLLKQA IYREENNVVDLFIILLRFSSLVSET
Sbjct: 1   MKINIDREARKVAVDNRIPLRNYFRIADNLLKQADIYREENNVVDLFIILLRFSSLVSET 60

Query: 61  IPRHRDYQIFFPKEKIFYKKKLLNVVDELESLKPEFDCRVNELEKVHAQPRLPPPDDQDR 120
           IPRHRDYQ FFPKEKIFYKKKLL+VVDELESLKPEFD RVNELEKV  +PRLPP D+Q  
Sbjct: 61  IPRHRDYQAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVSGRPRLPPSDEQGG 120

Query: 121 SSYTLGPSSMEWPSVNRDYSLVLDRKQHASLQPQSSWRYNHDQGQVSQSSSFKIANKFPN 180
            S +L PSSM  P  N +YSLVLD+KQHA+ QPQSSWRY+  +GQVSQSS  +I N+  N
Sbjct: 121 VSNSLRPSSMIQPYANGNYSLVLDQKQHANSQPQSSWRYHDGRGQVSQSSYSRIENRLSN 180

Query: 181 LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIHYPRNQDFTPEDPRLNQIEQCDLV 240
           LSLNVPLPKPETLSRHSILGPSG QGQWKGPSTDKIHYPRNQDFTP D RLNQIEQCDLV
Sbjct: 181 LSLNVPLPKPETLSRHSILGPSGFQGQWKGPSTDKIHYPRNQDFTPLDSRLNQIEQCDLV 240

Query: 241 AHKDNDSGDLMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 300
           AHKD+  G +MSMMESVLSLDDGRWPHPAD+S  PLITEVREDPFPLVKQPSPPPVLAKV
Sbjct: 241 AHKDSGPGGVMSMMESVLSLDDGRWPHPADISITPLITEVREDPFPLVKQPSPPPVLAKV 300

Query: 301 QQEYATIPPSKVADPRPGMAKQSQDGPDSFQRLQIPVKMLDDFLRVARENTNKNLETCGV 360
           QQEYATIPPSKVADPRPG+AKQS D  +SFQRL IPVKMLDDFLR+ARENTNKNLETCGV
Sbjct: 301 QQEYATIPPSKVADPRPGVAKQSLDESESFQRLHIPVKMLDDFLRLARENTNKNLETCGV 360

Query: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420
           LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM
Sbjct: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420

Query: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480
           SSVDLHTHYSYQIMLPEAIAIVMAPTD SSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP
Sbjct: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480

Query: 481 EDGSPLYEHCSHVLMNPNLKFDVVDLR 508
           EDGSPLYEHCSHVLMNPN KFDV+DLR
Sbjct: 481 EDGSPLYEHCSHVLMNPNAKFDVIDLR 507

[Close]

BLAST of MS019325 vs. ExPASy Swiss-Prot
Match: Q5PNU3 (AMSH-like ubiquitin thioesterase 3 OS=Arabidopsis thaliana OX=3702 GN=AMSH3 PE=1 SV=2)

HSP 1 Score: 580.1 bits (1494), Expect = 2.4e-164
Identity = 307/515 (59.61%), Postives = 385/515 (74.76%), Query Frame = 0


Query: 1   MKINIDREARKVTVDSRIPLRNYYRIADNLLKQASIYREENNVVDLFIILLRFSSLVSET 60
           MKI++++ AR++ VD+RIPLRNYYRIADNLL+QASIYREE NVVDL+I+LLR+SSL+SET
Sbjct: 1   MKIDLNKVAREIEVDNRIPLRNYYRIADNLLRQASIYREEKNVVDLYIMLLRYSSLISET 60

Query: 61  IPRHRDYQIFFPKEKIFYKKKLLNVVDELESLKPEFDCRVNELEKVHAQPRLPPPDDQDR 120
           IP HRDYQ   P+E++  +K+L  V++ELESLKPEF+  V++L +V  + R    D  D 
Sbjct: 61  IPFHRDYQASLPQERLGSRKRLRAVINELESLKPEFNQLVDKLNRVEDESR---QDGSDL 120

Query: 121 SSYTLGPSSMEWPSVNR-DYSLVLDRKQHASLQPQSSWRYNHDQGQVSQSSSFKIANKFP 180
              +    ++EWP  ++  YS     K   + QP  SW YN++    S S+  +I  +F 
Sbjct: 121 PVVSYSSDAVEWPPAHKASYSRPDINKPLPTSQP--SWTYNNN--LTSSSNRTQIDQQFQ 180

Query: 181 NLSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTD-KIHYPRNQDF-TPEDPRLNQIEQC 240
            LS +   P   TLSRHS LGP+GL+ Q   P ++ K+ YP N D+ + ++  L +    
Sbjct: 181 KLSFDFLPPNQATLSRHSFLGPNGLKRQMVAPKSEIKVQYPSNTDWGSADNSGLIEAGPS 240

Query: 241 DLVAHKDNDSGDLMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLV--KQPSPPP 300
              A  + DS + +S + SVLSLDDGRW   ++      I++  EDPF  V  KQPSPPP
Sbjct: 241 SSSASLNGDSQE-VSTLNSVLSLDDGRWQRHSEAVNSQFISDATEDPFQFVGMKQPSPPP 300

Query: 301 VLAKVQQEYATIPPSKVADPRPGMA---KQSQDGPDSFQRLQIPVKMLDDFLRVARENTN 360
           VLA+V QE A I PSKVADPRPG A    + ++G +S+Q L +PV+++DDFLR+AR NT 
Sbjct: 301 VLAQVHQELAQICPSKVADPRPGPAIPSLEGKEGSNSYQHLHVPVRIMDDFLRLARSNTE 360

Query: 361 KNLETCGVLAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHT 420
           +NLETCGVLAGSLKNRVF+I+TLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHT
Sbjct: 361 RNLETCGVLAGSLKNRVFHITTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHT 420

Query: 421 HPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQR 480
           HP+QTCFMSSVDLHTHYSYQIMLPEA+AIVMAPTD S+P+GIFHLSDP GVSVIRNCQQR
Sbjct: 421 HPTQTCFMSSVDLHTHYSYQIMLPEAVAIVMAPTDESTPHGIFHLSDPSGVSVIRNCQQR 480

Query: 481 GFHPHEEPEDGSPLYEHCSHVLMNPNLKFDVVDLR 508
           GFHPHEE EDG+P+YEHCSHV +N  LK++V+DLR
Sbjct: 481 GFHPHEESEDGNPIYEHCSHVFLNAKLKYEVLDLR 507

[Close]

BLAST of MS019325 vs. ExPASy Swiss-Prot
Match: Q8VYB5 (AMSH-like ubiquitin thioesterase 1 OS=Arabidopsis thaliana OX=3702 GN=AMSH1 PE=2 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 1.5e-121
Identity = 252/515 (48.93%), Postives = 335/515 (65.05%), Query Frame = 0


Query: 3   INIDREARKVTVDSRIPLRNYYRIADNLLKQASIYREENNVVDLFIILLRFSSLVSETIP 62
           I+I   AR++ VD+RI L+ Y+RIADN+LKQA+I+R E NV+DL+++LLRFSSL  ETIP
Sbjct: 8   IDIATSARRIGVDNRISLKFYFRIADNILKQANIFRAEKNVIDLYVMLLRFSSLALETIP 67

Query: 63  RHRDYQIFFPKEKIFYKKKLLNVVDELESLKPEFDCRVNELEKVHAQPRLPPPDDQDR-- 122
            HRDY+      K + + +LL+V+ ELE LKP    R++EL      P+L P  +     
Sbjct: 68  SHRDYRTSLKSNKEYLRMRLLDVLTELEKLKPVVQQRIDEL-----YPKLKPRYNVQAHP 127

Query: 123 SSYTLGPSSMEWPSVNR-DYSLVLDRKQHASLQPQSSWRYNHDQGQVSQSSSFKIANKFP 182
           ++ +LG SS   PS N  D++ V +   H S        Y   +GQ   +++  +  +F 
Sbjct: 128 ANGSLGWSSAVKPSFNSYDHAKVRNPPGHNS-------GYMGSRGQQFLNAA-PLEERFR 187

Query: 183 NLSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTD-KIHYPRNQDFTP-EDPRLNQ-IEQ 242
            +S+N   P  ETLS+HSILGP GL  QW+ P  D K+ YP N DF+P   P   Q ++ 
Sbjct: 188 KMSVNF-RPNEETLSKHSILGPGGLSAQWQPPKYDTKVQYPSNIDFSPVVIPSFQQLVDS 247

Query: 243 CDLVAHKDNDSGDLMSMMESVLSLDDGRWPHPADMSTPPLITEVRE-DPFPLVKQPSPPP 302
             ++ +  ND  +   +  SV S +  +  +  ++S+     E    +   L++QPSPPP
Sbjct: 248 KPMITNGSNDEPEKPIVEPSVASNEKIQKNYTEELSSMISFEEPESVNENNLIRQPSPPP 307

Query: 303 VLAKVQQEYATIPPSKVADPRPGMAKQSQD---GPDSFQRLQIPVKMLDDFLRVARENTN 362
           VLA+VQ     + P +V +P   +     D     +S   L I   M+D F+R+A+ NT 
Sbjct: 308 VLAEVQDLVPALCP-EVREPECMIENSLPDESLRSESPLELHIATSMMDTFMRLAKSNTK 367

Query: 363 KNLETCGVLAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHT 422
           KNLETCG+LAGSLKNR FYI+ LIIPKQESTSDSCQ  NEEEIFEVQD+ SLFPLGWIHT
Sbjct: 368 KNLETCGILAGSLKNRKFYITALIIPKQESTSDSCQATNEEEIFEVQDKQSLFPLGWIHT 427

Query: 423 HPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQR 482
           HP+Q+CFMSS+D+HTHYSYQIMLPEA+AIVMAP D+S  +GIF L+ PGG++VIRNC +R
Sbjct: 428 HPTQSCFMSSIDVHTHYSYQIMLPEAVAIVMAPQDSSRNHGIFRLTTPGGMTVIRNCDRR 487

Query: 483 GFHPHEEPEDGSPLYEHCSHVLMNPNLKFDVVDLR 508
           GFH H  PEDG P+Y  C  V MNPNLKFDV+DLR
Sbjct: 488 GFHAHSSPEDGGPIYNTCKEVYMNPNLKFDVIDLR 507

[Close]

BLAST of MS019325 vs. ExPASy Swiss-Prot
Match: Q6NKP9 (AMSH-like ubiquitin thioesterase 2 OS=Arabidopsis thaliana OX=3702 GN=AMSH2 PE=2 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 3.9e-69
Identity = 113/175 (64.57%), Postives = 145/175 (82.86%), Query Frame = 0


Query: 333 LQIPVKMLDDFLRVARENTNKNLETCGVLAGSLKNRVFYISTLIIPKQESTSDSCQTLNE 392
           + I  ++L+DF  +ARENT K+LETCG LA  L+  +FY++TLIIPKQESTS+SCQ +NE
Sbjct: 49  VHISERLLEDFTELARENTEKDLETCGTLAAFLERGIFYVTTLIIPKQESTSNSCQAMNE 108

Query: 393 EEIFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPY 452
            E+F +Q+   L+P+GWIHTHPSQ CFMSSVDLHTHYSYQ+M+PEA AIV+APTD+S  Y
Sbjct: 109 VEVFSIQNERELYPVGWIHTHPSQGCFMSSVDLHTHYSYQVMVPEAFAIVVAPTDSSKSY 168

Query: 453 GIFHLSDPGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNLKFDVVDLR 508
           GIF L+DPGG+ V+R C + GFHPH+EPEDG+P+YEHCS+V  N NL+F++ DLR
Sbjct: 169 GIFKLTDPGGMEVLRGCSETGFHPHKEPEDGNPVYEHCSNVYKNSNLRFEIFDLR 223

[Close]

BLAST of MS019325 vs. ExPASy Swiss-Prot
Match: O95630 (STAM-binding protein OS=Homo sapiens OX=9606 GN=STAMBP PE=1 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 2.3e-45
Identity = 149/485 (30.72%), Postives = 223/485 (45.98%), Query Frame = 0


Query: 12  VTVDSRIPLRNYYRIADNLLKQASIYREENNVVDLFIILLRFSSLVSETIPRHRDYQIFF 71
           V V+  IP R Y+R    +++ ASIY EE N+   FI+  ++ +L  E +P+HRDY+   
Sbjct: 25  VEVNEDIPPRRYFRSGVEIIRMASIYSEEGNIEHAFILYNKYITLFIEKLPKHRDYKSAV 84

Query: 72  PKEKIFYKKKLLNVV-DELESLKPEFDCRVNELEKVHAQPRLPPPDDQDRSSYTLGPSSM 131
             EK    KKL  +   + E LK        EL K + +      +++ + +  L  +  
Sbjct: 85  IPEKKDTVKKLKEIAFPKAEELKA-------ELLKRYTKEYTEYNEEKKKEAEELARNMA 144

Query: 132 EWPSVNRDYSLVLDRKQHASLQPQSSWRYNHDQGQVSQSSSFKIANKFPNLSLNVPLPKP 191
               + ++   V  +KQ    Q Q                     + F  +  N  L K 
Sbjct: 145 IQQELEKEKQRVAQQKQQQLEQEQ--------------------FHAFEEMIRNQELEK- 204

Query: 192 ETLSRHSILGPSGLQGQWKGPSTDKIHYPRNQDFTPEDPRLNQIEQCDLVAHKDNDSGDL 251
               R  I+                      Q+F   DP L       LV   +  S D+
Sbjct: 205 ---ERLKIV----------------------QEFGKVDPGLGG----PLVPDLEKPSLDV 264

Query: 252 MSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLVKQPSPPPVLAKVQQEYATIPPS 311
              + +V S+       P+D  T                +P+ PPV+ +           
Sbjct: 265 FPTL-TVSSI------QPSDCHT--------------TVRPAKPPVVDR----------- 324

Query: 312 KVADPRPGMAKQSQDGP--DSFQRLQIPVKMLDDFLRVARENTNKNLETCGVLAGSLKNR 371
                +PG    S+  P  D  + + +P ++   FL++A  NT + +ETCG+L G L   
Sbjct: 325 ---SLKPGALSNSESIPTIDGLRHVVVPGRLCPQFLQLASANTARGVETCGILCGKLMRN 384

Query: 372 VFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTH 431
            F I+ ++IPKQ + SD C T NEEE+F +QD+  L  LGWIHTHP+QT F+SSVDLHTH
Sbjct: 385 EFTITHVLIPKQSAGSDYCNTENEEELFLIQDQQGLITLGWIHTHPTQTAFLSSVDLHTH 411

Query: 432 YSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEPEDGSPLYE 491
            SYQ+MLPE++AIV +P    +  G F L+D  G+  I +C+Q+GFHPH +     PL+ 
Sbjct: 445 CSYQMMLPESVAIVCSPKFQET--GFFKLTD-HGLEEISSCRQKGFHPHSK---DPPLFC 411

Query: 492 HCSHV 494
            CSHV
Sbjct: 505 SCSHV 411

[Close]

BLAST of MS019325 vs. ExPASy Swiss-Prot
Match: Q9P371 (AMSH-like protease sst2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sst2 PE=1 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 3.0e-45
Identity = 100/204 (49.02%), Postives = 130/204 (63.73%), Query Frame = 0


Query: 310 SKVADPRPGMAKQSQ-----DGPDSFQRLQIPVKMLDDFLRVARENTNKNLETCGVLAGS 369
           ++ ++PRP  A   +     +G    + + +P  +   FL V + NT KNLETCG+L G 
Sbjct: 235 TRTSEPRPKPAGTFKIHAYTEGGKPLRTIYLPKLLKKVFLDVVKPNTKKNLETCGILCGK 294

Query: 370 LKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFMSSVD 429
           L+   F+I+ L+IP QE+TSD+C T +E  +FE QD+ +L  LGWIHTHP+QTCFMSSVD
Sbjct: 295 LRQNAFFITHLVIPLQEATSDTCGTTDEASLFEFQDKHNLLTLGWIHTHPTQTCFMSSVD 354

Query: 430 LHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRG-FHPHEEPEDG 489
           LHTH SYQ+MLPEAIAIVMAP+  +S  GIF L DP G+  I  C++ G FHPHE     
Sbjct: 355 LHTHCSYQLMLPEAIAIVMAPSKNTS--GIFRLLDPEGLQTIVKCRKPGLFHPHEGKV-- 414

Query: 490 SPLYEHCSHVLMNPNLKFDVVDLR 508
             +     HV    N K  VVDLR
Sbjct: 415 YTMVAQPGHV-REINSKLQVVDLR 433

[Close]

BLAST of MS019325 vs. ExPASy TrEMBL
Match: A0A6J1CAL3 (AMSH-like ubiquitin thioesterase 3 OS=Momordica charantia OX=3673 GN=LOC111009837 PE=3 SV=1)

HSP 1 Score: 1027.7 bits (2656), Expect = 1.6e-296
Identity = 505/507 (99.61%), Postives = 505/507 (99.61%), Query Frame = 0


Query: 1   MKINIDREARKVTVDSRIPLRNYYRIADNLLKQASIYREENNVVDLFIILLRFSSLVSET 60
           MKINIDREARKVTVDSRIPLRNYYRIADNLLKQASIYREENNVVDLFIILLRFSSLVSET
Sbjct: 1   MKINIDREARKVTVDSRIPLRNYYRIADNLLKQASIYREENNVVDLFIILLRFSSLVSET 60

Query: 61  IPRHRDYQIFFPKEKIFYKKKLLNVVDELESLKPEFDCRVNELEKVHAQPRLPPPDDQDR 120
           IPRHRDYQIFFPKEKIFYKKKLLNVVDELESLKPEFDCRVNELEKVHAQPRLPPPDDQDR
Sbjct: 61  IPRHRDYQIFFPKEKIFYKKKLLNVVDELESLKPEFDCRVNELEKVHAQPRLPPPDDQDR 120

Query: 121 SSYTLGPSSMEWPSVNRDYSLVLDRKQHASLQPQSSWRYNHDQGQVSQSSSFKIANKFPN 180
           SSYTLGPSSMEWPSVNRDYSLVLDRKQHASLQPQSSWRYNHDQGQVSQSSSFKIANKFPN
Sbjct: 121 SSYTLGPSSMEWPSVNRDYSLVLDRKQHASLQPQSSWRYNHDQGQVSQSSSFKIANKFPN 180

Query: 181 LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIHYPRNQDFTPEDPRLNQIEQCDLV 240
           LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIHYPRNQDFTPEDPRLNQIEQCDLV
Sbjct: 181 LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIHYPRNQDFTPEDPRLNQIEQCDLV 240

Query: 241 AHKDNDSGDLMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 300
           AHKDN SG LMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV
Sbjct: 241 AHKDNYSGGLMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 300

Query: 301 QQEYATIPPSKVADPRPGMAKQSQDGPDSFQRLQIPVKMLDDFLRVARENTNKNLETCGV 360
           QQEYATIPPSKVADPRPGMAKQSQDGPDSFQRLQIPVKMLDDFLRVARENTNKNLETCGV
Sbjct: 301 QQEYATIPPSKVADPRPGMAKQSQDGPDSFQRLQIPVKMLDDFLRVARENTNKNLETCGV 360

Query: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420
           LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM
Sbjct: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420

Query: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480
           SSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP
Sbjct: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480

Query: 481 EDGSPLYEHCSHVLMNPNLKFDVVDLR 508
           EDGSPLYEHCSHVLMNPNLKFDVVDLR
Sbjct: 481 EDGSPLYEHCSHVLMNPNLKFDVVDLR 507

[Close]

BLAST of MS019325 vs. ExPASy TrEMBL
Match: A0A5D3BT78 (AMSH-like ubiquitin thioesterase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00210 PE=3 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 7.9e-259
Identity = 443/507 (87.38%), Postives = 472/507 (93.10%), Query Frame = 0


Query: 1   MKINIDREARKVTVDSRIPLRNYYRIADNLLKQASIYREENNVVDLFIILLRFSSLVSET 60
           MKINIDREARKV VD+RIPLRNY+RIADNLLKQA+IYREENNVVDLFIILLRFSSLVSET
Sbjct: 54  MKINIDREARKVAVDNRIPLRNYFRIADNLLKQANIYREENNVVDLFIILLRFSSLVSET 113

Query: 61  IPRHRDYQIFFPKEKIFYKKKLLNVVDELESLKPEFDCRVNELEKVHAQPRLPPPDDQDR 120
           IPRHRDYQ FFPKEKI+YKKKLLNVVDELESLKPEFD RVNELEKV+ +PRLP PD+Q  
Sbjct: 114 IPRHRDYQAFFPKEKIYYKKKLLNVVDELESLKPEFDRRVNELEKVYGRPRLPLPDEQGS 173

Query: 121 SSYTLGPSSMEWPSVNRDYSLVLDRKQHASLQPQSSWRYNHDQGQVSQSSSFKIANKFPN 180
            S +L PS  E P  N +YSL+LD+KQH + +PQSSWRY+ D+GQVSQSSS ++ NKF  
Sbjct: 174 VSNSLRPSPKEQPYANGNYSLMLDQKQHVNSRPQSSWRYSDDRGQVSQSSSLRLENKFST 233

Query: 181 LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIHYPRNQDFTPEDPRLNQIEQCDLV 240
           +SLNVPLPKPETLSRHSILGPSGLQ QW GPSTDKI YPRNQDFTPED RLNQ E CDLV
Sbjct: 234 MSLNVPLPKPETLSRHSILGPSGLQCQWMGPSTDKIQYPRNQDFTPEDSRLNQSEHCDLV 293

Query: 241 AHKDNDSGDLMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 300
           AHKD+ SG +MSMMESVLSLDDGRWP+PADMSTPPLITEVREDPFPLVKQPSPPPVLAKV
Sbjct: 294 AHKDSGSGGVMSMMESVLSLDDGRWPNPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 353

Query: 301 QQEYATIPPSKVADPRPGMAKQSQDGPDSFQRLQIPVKMLDDFLRVARENTNKNLETCGV 360
           QQEYATIPPSKVADPRPG+A+QSQD  +SFQRL IPVKMLDDFLR+ARENTNKNLETCGV
Sbjct: 354 QQEYATIPPSKVADPRPGVARQSQDESESFQRLHIPVKMLDDFLRLARENTNKNLETCGV 413

Query: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420
           LAGSLKNR+FYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM
Sbjct: 414 LAGSLKNRIFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 473

Query: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480
           SSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP
Sbjct: 474 SSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 533

Query: 481 EDGSPLYEHCSHVLMNPNLKFDVVDLR 508
           +DGSPLYEHCSHV+MNPN KFDV+DLR
Sbjct: 534 DDGSPLYEHCSHVMMNPNAKFDVIDLR 560

[Close]

BLAST of MS019325 vs. ExPASy TrEMBL
Match: A0A1S3C8Z8 (LOW QUALITY PROTEIN: AMSH-like ubiquitin thioesterase 3 OS=Cucumis melo OX=3656 GN=LOC103497805 PE=3 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 7.9e-259
Identity = 443/507 (87.38%), Postives = 472/507 (93.10%), Query Frame = 0


Query: 1   MKINIDREARKVTVDSRIPLRNYYRIADNLLKQASIYREENNVVDLFIILLRFSSLVSET 60
           MKINIDREARKV VD+RIPLRNY+RIADNLLKQA+IYREENNVVDLFIILLRFSSLVSET
Sbjct: 1   MKINIDREARKVAVDNRIPLRNYFRIADNLLKQANIYREENNVVDLFIILLRFSSLVSET 60

Query: 61  IPRHRDYQIFFPKEKIFYKKKLLNVVDELESLKPEFDCRVNELEKVHAQPRLPPPDDQDR 120
           IPRHRDYQ FFPKEKI+YKKKLLNVVDELESLKPEFD RVNELEKV+ +PRLP PD+Q  
Sbjct: 61  IPRHRDYQAFFPKEKIYYKKKLLNVVDELESLKPEFDRRVNELEKVYGRPRLPLPDEQGS 120

Query: 121 SSYTLGPSSMEWPSVNRDYSLVLDRKQHASLQPQSSWRYNHDQGQVSQSSSFKIANKFPN 180
            S +L PS  E P  N +YSL+LD+KQH + +PQSSWRY+ D+GQVSQSSS ++ NKF  
Sbjct: 121 VSNSLRPSPKEQPYANGNYSLMLDQKQHVNSRPQSSWRYSDDRGQVSQSSSLRLENKFST 180

Query: 181 LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIHYPRNQDFTPEDPRLNQIEQCDLV 240
           +SLNVPLPKPETLSRHSILGPSGLQ QW GPSTDKI YPRNQDFTPED RLNQ E CDLV
Sbjct: 181 MSLNVPLPKPETLSRHSILGPSGLQCQWMGPSTDKIQYPRNQDFTPEDSRLNQSEHCDLV 240

Query: 241 AHKDNDSGDLMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 300
           AHKD+ SG +MSMMESVLSLDDGRWP+PADMSTPPLITEVREDPFPLVKQPSPPPVLAKV
Sbjct: 241 AHKDSGSGGVMSMMESVLSLDDGRWPNPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 300

Query: 301 QQEYATIPPSKVADPRPGMAKQSQDGPDSFQRLQIPVKMLDDFLRVARENTNKNLETCGV 360
           QQEYATIPPSKVADPRPG+A+QSQD  +SFQRL IPVKMLDDFLR+ARENTNKNLETCGV
Sbjct: 301 QQEYATIPPSKVADPRPGVARQSQDESESFQRLHIPVKMLDDFLRLARENTNKNLETCGV 360

Query: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420
           LAGSLKNR+FYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM
Sbjct: 361 LAGSLKNRIFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420

Query: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480
           SSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP
Sbjct: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480

Query: 481 EDGSPLYEHCSHVLMNPNLKFDVVDLR 508
           +DGSPLYEHCSHV+MNPN KFDV+DLR
Sbjct: 481 DDGSPLYEHCSHVMMNPNAKFDVIDLR 507

[Close]

BLAST of MS019325 vs. ExPASy TrEMBL
Match: A0A6J1L013 (AMSH-like ubiquitin thioesterase 3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499142 PE=3 SV=1)

HSP 1 Score: 901.7 bits (2329), Expect = 1.4e-258
Identity = 448/507 (88.36%), Postives = 468/507 (92.31%), Query Frame = 0


Query: 1   MKINIDREARKVTVDSRIPLRNYYRIADNLLKQASIYREENNVVDLFIILLRFSSLVSET 60
           MKINIDREARKV VD+RIPLRNY+RIADNLLKQA IYREENNVVDLFIILLRFSSLVSET
Sbjct: 1   MKINIDREARKVAVDNRIPLRNYFRIADNLLKQADIYREENNVVDLFIILLRFSSLVSET 60

Query: 61  IPRHRDYQIFFPKEKIFYKKKLLNVVDELESLKPEFDCRVNELEKVHAQPRLPPPDDQDR 120
           IPRHRDYQ FFPKEKIFYKKKLL+VVDELESLKPEFD RVNELEKV  +PRLPP D+Q  
Sbjct: 61  IPRHRDYQAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVSGRPRLPPSDEQGG 120

Query: 121 SSYTLGPSSMEWPSVNRDYSLVLDRKQHASLQPQSSWRYNHDQGQVSQSSSFKIANKFPN 180
            S +L PSSM  P  N +YSLVLD+KQHA+ QPQSSWRY+  +GQVSQSS  +I N+  N
Sbjct: 121 VSNSLRPSSMIQPYANGNYSLVLDQKQHANSQPQSSWRYHDGRGQVSQSSYSRIENRLSN 180

Query: 181 LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIHYPRNQDFTPEDPRLNQIEQCDLV 240
           LSLNVPLPKPETLSRHSILGPSG QGQWKGPSTDKIHYPRNQDFTP D RLNQIEQCDLV
Sbjct: 181 LSLNVPLPKPETLSRHSILGPSGFQGQWKGPSTDKIHYPRNQDFTPLDSRLNQIEQCDLV 240

Query: 241 AHKDNDSGDLMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 300
           AHKD+  G +MSMMESVLSLDDGRWPHPAD+S  PLITEVREDPFPLVKQPSPPPVLAKV
Sbjct: 241 AHKDSGPGGVMSMMESVLSLDDGRWPHPADISITPLITEVREDPFPLVKQPSPPPVLAKV 300

Query: 301 QQEYATIPPSKVADPRPGMAKQSQDGPDSFQRLQIPVKMLDDFLRVARENTNKNLETCGV 360
           QQEYATIPPSKVADPRPG+AKQS D  +SFQRL IPVKMLDDFLR+ARENTNKNLETCGV
Sbjct: 301 QQEYATIPPSKVADPRPGVAKQSLDESESFQRLHIPVKMLDDFLRLARENTNKNLETCGV 360

Query: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420
           LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM
Sbjct: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420

Query: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480
           SSVDLHTHYSYQIMLPEAIAIVMAPTD SSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP
Sbjct: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480

Query: 481 EDGSPLYEHCSHVLMNPNLKFDVVDLR 508
           EDGSPLYEHCSHVLMNPN KFDV+DLR
Sbjct: 481 EDGSPLYEHCSHVLMNPNAKFDVIDLR 507

[Close]

BLAST of MS019325 vs. ExPASy TrEMBL
Match: A0A0A0KG71 (MPN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G311020 PE=3 SV=1)

HSP 1 Score: 890.6 bits (2300), Expect = 3.1e-255
Identity = 440/507 (86.79%), Postives = 467/507 (92.11%), Query Frame = 0


Query: 1   MKINIDREARKVTVDSRIPLRNYYRIADNLLKQASIYREENNVVDLFIILLRFSSLVSET 60
           MKINIDREARKV VD+RIPLRNY+RIADNLLKQA+IYREENNVVDLFIILLRFSSLVSET
Sbjct: 1   MKINIDREARKVAVDNRIPLRNYFRIADNLLKQANIYREENNVVDLFIILLRFSSLVSET 60

Query: 61  IPRHRDYQIFFPKEKIFYKKKLLNVVDELESLKPEFDCRVNELEKVHAQPRLPPPDDQDR 120
           IPRHRDY  FFP EKI+YKKKLLNVVDELESLKPEFD RVNELEKV+ +PRLPPPD+Q  
Sbjct: 61  IPRHRDYPAFFPNEKIYYKKKLLNVVDELESLKPEFDRRVNELEKVYGRPRLPPPDEQGS 120

Query: 121 SSYTLGPSSMEWPSVNRDYSLVLDRKQHASLQPQSSWRYNHDQGQVSQSSSFKIANKFPN 180
            S +  PS  E P VN +YSL+LD+KQH   +PQSS RY+ D+GQVS+SSS ++ NKF  
Sbjct: 121 VSNSFRPSPKEQPYVNGNYSLMLDQKQHVHSRPQSSRRYSDDRGQVSESSSLRLENKFST 180

Query: 181 LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIHYPRNQDFTPEDPRLNQIEQCDLV 240
           LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKI YP NQDFT ED RLNQ E CDLV
Sbjct: 181 LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIQYPINQDFTLEDSRLNQNEHCDLV 240

Query: 241 AHKDNDSGDLMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 300
           AHKD+  G +MS+MESVLSLDDGRWPHPADMS PPLITEVREDPFPLVKQPSPPPVLAKV
Sbjct: 241 AHKDSGPGGVMSVMESVLSLDDGRWPHPADMSIPPLITEVREDPFPLVKQPSPPPVLAKV 300

Query: 301 QQEYATIPPSKVADPRPGMAKQSQDGPDSFQRLQIPVKMLDDFLRVARENTNKNLETCGV 360
           QQEYATIPPSKVADPRPG+A+QSQD  +SFQRL IPVKMLDDFLR+ARENTNKNLETCGV
Sbjct: 301 QQEYATIPPSKVADPRPGVARQSQDESESFQRLHIPVKMLDDFLRLARENTNKNLETCGV 360

Query: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420
           LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM
Sbjct: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420

Query: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480
           SSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP
Sbjct: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480

Query: 481 EDGSPLYEHCSHVLMNPNLKFDVVDLR 508
           +DGSPLYEHCSHV+MNPN KFDV+DLR
Sbjct: 481 DDGSPLYEHCSHVMMNPNAKFDVMDLR 507

[Close]

BLAST of MS019325 vs. TAIR 10
Match: AT4G16144.1 (associated molecule with the SH3 domain of STAM 3 )

HSP 1 Score: 580.1 bits (1494), Expect = 1.7e-165
Identity = 307/515 (59.61%), Postives = 385/515 (74.76%), Query Frame = 0


Query: 1   MKINIDREARKVTVDSRIPLRNYYRIADNLLKQASIYREENNVVDLFIILLRFSSLVSET 60
           MKI++++ AR++ VD+RIPLRNYYRIADNLL+QASIYREE NVVDL+I+LLR+SSL+SET
Sbjct: 1   MKIDLNKVAREIEVDNRIPLRNYYRIADNLLRQASIYREEKNVVDLYIMLLRYSSLISET 60

Query: 61  IPRHRDYQIFFPKEKIFYKKKLLNVVDELESLKPEFDCRVNELEKVHAQPRLPPPDDQDR 120
           IP HRDYQ   P+E++  +K+L  V++ELESLKPEF+  V++L +V  + R    D  D 
Sbjct: 61  IPFHRDYQASLPQERLGSRKRLRAVINELESLKPEFNQLVDKLNRVEDESR---QDGSDL 120

Query: 121 SSYTLGPSSMEWPSVNR-DYSLVLDRKQHASLQPQSSWRYNHDQGQVSQSSSFKIANKFP 180
              +    ++EWP  ++  YS     K   + QP  SW YN++    S S+  +I  +F 
Sbjct: 121 PVVSYSSDAVEWPPAHKASYSRPDINKPLPTSQP--SWTYNNN--LTSSSNRTQIDQQFQ 180

Query: 181 NLSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTD-KIHYPRNQDF-TPEDPRLNQIEQC 240
            LS +   P   TLSRHS LGP+GL+ Q   P ++ K+ YP N D+ + ++  L +    
Sbjct: 181 KLSFDFLPPNQATLSRHSFLGPNGLKRQMVAPKSEIKVQYPSNTDWGSADNSGLIEAGPS 240

Query: 241 DLVAHKDNDSGDLMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLV--KQPSPPP 300
              A  + DS + +S + SVLSLDDGRW   ++      I++  EDPF  V  KQPSPPP
Sbjct: 241 SSSASLNGDSQE-VSTLNSVLSLDDGRWQRHSEAVNSQFISDATEDPFQFVGMKQPSPPP 300

Query: 301 VLAKVQQEYATIPPSKVADPRPGMA---KQSQDGPDSFQRLQIPVKMLDDFLRVARENTN 360
           VLA+V QE A I PSKVADPRPG A    + ++G +S+Q L +PV+++DDFLR+AR NT 
Sbjct: 301 VLAQVHQELAQICPSKVADPRPGPAIPSLEGKEGSNSYQHLHVPVRIMDDFLRLARSNTE 360

Query: 361 KNLETCGVLAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHT 420
           +NLETCGVLAGSLKNRVF+I+TLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHT
Sbjct: 361 RNLETCGVLAGSLKNRVFHITTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHT 420

Query: 421 HPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQR 480
           HP+QTCFMSSVDLHTHYSYQIMLPEA+AIVMAPTD S+P+GIFHLSDP GVSVIRNCQQR
Sbjct: 421 HPTQTCFMSSVDLHTHYSYQIMLPEAVAIVMAPTDESTPHGIFHLSDPSGVSVIRNCQQR 480

Query: 481 GFHPHEEPEDGSPLYEHCSHVLMNPNLKFDVVDLR 508
           GFHPHEE EDG+P+YEHCSHV +N  LK++V+DLR
Sbjct: 481 GFHPHEESEDGNPIYEHCSHVFLNAKLKYEVLDLR 507

[Close]

BLAST of MS019325 vs. TAIR 10
Match: AT1G48790.1 (associated molecule with the SH3 domain of STAM 1 )

HSP 1 Score: 438.0 bits (1125), Expect = 1.1e-122
Identity = 252/515 (48.93%), Postives = 335/515 (65.05%), Query Frame = 0


Query: 3   INIDREARKVTVDSRIPLRNYYRIADNLLKQASIYREENNVVDLFIILLRFSSLVSETIP 62
           I+I   AR++ VD+RI L+ Y+RIADN+LKQA+I+R E NV+DL+++LLRFSSL  ETIP
Sbjct: 8   IDIATSARRIGVDNRISLKFYFRIADNILKQANIFRAEKNVIDLYVMLLRFSSLALETIP 67

Query: 63  RHRDYQIFFPKEKIFYKKKLLNVVDELESLKPEFDCRVNELEKVHAQPRLPPPDDQDR-- 122
            HRDY+      K + + +LL+V+ ELE LKP    R++EL      P+L P  +     
Sbjct: 68  SHRDYRTSLKSNKEYLRMRLLDVLTELEKLKPVVQQRIDEL-----YPKLKPRYNVQAHP 127

Query: 123 SSYTLGPSSMEWPSVNR-DYSLVLDRKQHASLQPQSSWRYNHDQGQVSQSSSFKIANKFP 182
           ++ +LG SS   PS N  D++ V +   H S        Y   +GQ   +++  +  +F 
Sbjct: 128 ANGSLGWSSAVKPSFNSYDHAKVRNPPGHNS-------GYMGSRGQQFLNAA-PLEERFR 187

Query: 183 NLSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTD-KIHYPRNQDFTP-EDPRLNQ-IEQ 242
            +S+N   P  ETLS+HSILGP GL  QW+ P  D K+ YP N DF+P   P   Q ++ 
Sbjct: 188 KMSVNF-RPNEETLSKHSILGPGGLSAQWQPPKYDTKVQYPSNIDFSPVVIPSFQQLVDS 247

Query: 243 CDLVAHKDNDSGDLMSMMESVLSLDDGRWPHPADMSTPPLITEVRE-DPFPLVKQPSPPP 302
             ++ +  ND  +   +  SV S +  +  +  ++S+     E    +   L++QPSPPP
Sbjct: 248 KPMITNGSNDEPEKPIVEPSVASNEKIQKNYTEELSSMISFEEPESVNENNLIRQPSPPP 307

Query: 303 VLAKVQQEYATIPPSKVADPRPGMAKQSQD---GPDSFQRLQIPVKMLDDFLRVARENTN 362
           VLA+VQ     + P +V +P   +     D     +S   L I   M+D F+R+A+ NT 
Sbjct: 308 VLAEVQDLVPALCP-EVREPECMIENSLPDESLRSESPLELHIATSMMDTFMRLAKSNTK 367

Query: 363 KNLETCGVLAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHT 422
           KNLETCG+LAGSLKNR FYI+ LIIPKQESTSDSCQ  NEEEIFEVQD+ SLFPLGWIHT
Sbjct: 368 KNLETCGILAGSLKNRKFYITALIIPKQESTSDSCQATNEEEIFEVQDKQSLFPLGWIHT 427

Query: 423 HPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQR 482
           HP+Q+CFMSS+D+HTHYSYQIMLPEA+AIVMAP D+S  +GIF L+ PGG++VIRNC +R
Sbjct: 428 HPTQSCFMSSIDVHTHYSYQIMLPEAVAIVMAPQDSSRNHGIFRLTTPGGMTVIRNCDRR 487

Query: 483 GFHPHEEPEDGSPLYEHCSHVLMNPNLKFDVVDLR 508
           GFH H  PEDG P+Y  C  V MNPNLKFDV+DLR
Sbjct: 488 GFHAHSSPEDGGPIYNTCKEVYMNPNLKFDVIDLR 507

[Close]

BLAST of MS019325 vs. TAIR 10
Match: AT1G10600.1 (associated molecule with the SH3 domain of STAM 2 )

HSP 1 Score: 263.8 bits (673), Expect = 2.8e-70
Identity = 113/175 (64.57%), Postives = 145/175 (82.86%), Query Frame = 0


Query: 333 LQIPVKMLDDFLRVARENTNKNLETCGVLAGSLKNRVFYISTLIIPKQESTSDSCQTLNE 392
           + I  ++L+DF  +ARENT K+LETCG LA  L+  +FY++TLIIPKQESTS+SCQ +NE
Sbjct: 49  VHISERLLEDFTELARENTEKDLETCGTLAAFLERGIFYVTTLIIPKQESTSNSCQAMNE 108

Query: 393 EEIFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPY 452
            E+F +Q+   L+P+GWIHTHPSQ CFMSSVDLHTHYSYQ+M+PEA AIV+APTD+S  Y
Sbjct: 109 VEVFSIQNERELYPVGWIHTHPSQGCFMSSVDLHTHYSYQVMVPEAFAIVVAPTDSSKSY 168

Query: 453 GIFHLSDPGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNLKFDVVDLR 508
           GIF L+DPGG+ V+R C + GFHPH+EPEDG+P+YEHCS+V  N NL+F++ DLR
Sbjct: 169 GIFKLTDPGGMEVLRGCSETGFHPHKEPEDGNPVYEHCSNVYKNSNLRFEIFDLR 223

[Close]

BLAST of MS019325 vs. TAIR 10
Match: AT1G10600.3 (associated molecule with the SH3 domain of STAM 2 )

HSP 1 Score: 260.0 bits (663), Expect = 4.0e-69
Identity = 114/175 (65.14%), Postives = 145/175 (82.86%), Query Frame = 0


Query: 333 LQIPVKMLDDFLRVARENTNKNLETCGVLAGSLKNRVFYISTLIIPKQESTSDSCQTLNE 392
           + I  ++L+DF  +ARENT K+LETCG LA  L+  +FY++TLIIPKQESTS+SCQ +NE
Sbjct: 49  VHISERLLEDFTELARENTEKDLETCGTLAAFLERGIFYVTTLIIPKQESTSNSCQAMNE 108

Query: 393 EEIFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPY 452
            E+F +Q+   L+P+GWIHTHPSQ CFMSSVDLHTHYSYQ+M+PEA AIV+APTD SS Y
Sbjct: 109 VEVFSIQNERELYPVGWIHTHPSQGCFMSSVDLHTHYSYQVMVPEAFAIVVAPTD-SSNY 168

Query: 453 GIFHLSDPGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNLKFDVVDLR 508
           GIF L+DPGG+ V+R C + GFHPH+EPEDG+P+YEHCS+V  N NL+F++ DLR
Sbjct: 169 GIFKLTDPGGMEVLRGCSETGFHPHKEPEDGNPVYEHCSNVYKNSNLRFEIFDLR 222

[Close]

BLAST of MS019325 vs. TAIR 10
Match: AT1G10600.2 (associated molecule with the SH3 domain of STAM 2 )

HSP 1 Score: 197.2 bits (500), Expect = 3.2e-50
Identity = 81/121 (66.94%), Postives = 102/121 (84.30%), Query Frame = 0


Query: 387 CQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPT 446
           CQ +NE E+F +Q+   L+P+GWIHTHPSQ CFMSSVDLHTHYSYQ+M+PEA AIV+APT
Sbjct: 46  CQAMNEVEVFSIQNERELYPVGWIHTHPSQGCFMSSVDLHTHYSYQVMVPEAFAIVVAPT 105

Query: 447 DTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNLKFDVVDL 506
           D+S  YGIF L+DPGG+ V+R C + GFHPH+EPEDG+P+YEHCS+V  N NL+F++ DL
Sbjct: 106 DSSKSYGIFKLTDPGGMEVLRGCSETGFHPHKEPEDGNPVYEHCSNVYKNSNLRFEIFDL 165

Query: 507 R 508
           R
Sbjct: 166 R 166

The following BLAST results are available for this feature:

BLAST of MS019325 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022138746.1	3.4e-296	99.61	AMSH-like ubiquitin thioesterase 3 [Momordica charantia]	[more]
XP_038875747.1	4.9e-263	89.15	AMSH-like ubiquitin thioesterase 3 [Benincasa hispida]	[more]
TYK02941.1	1.6e-258	87.38	AMSH-like ubiquitin thioesterase 3 [Cucumis melo var. makuwa]	[more]
XP_008458380.1	1.6e-258	87.38	PREDICTED: LOW QUALITY PROTEIN: AMSH-like ubiquitin thioesterase 3 [Cucumis melo...	[more]
XP_023006405.1	2.8e-258	88.36	AMSH-like ubiquitin thioesterase 3 isoform X1 [Cucurbita maxima]	[more]

BLAST of MS019325 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5PNU3	2.4e-164	59.61	AMSH-like ubiquitin thioesterase 3 OS=Arabidopsis thaliana OX=3702 GN=AMSH3 PE=1...	[more]
Q8VYB5	1.5e-121	48.93	AMSH-like ubiquitin thioesterase 1 OS=Arabidopsis thaliana OX=3702 GN=AMSH1 PE=2...	[more]
Q6NKP9	3.9e-69	64.57	AMSH-like ubiquitin thioesterase 2 OS=Arabidopsis thaliana OX=3702 GN=AMSH2 PE=2...	[more]
O95630	2.3e-45	30.72	STAM-binding protein OS=Homo sapiens OX=9606 GN=STAMBP PE=1 SV=1	[more]
Q9P371	3.0e-45	49.02	AMSH-like protease sst2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) O...	[more]

BLAST of MS019325 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CAL3	1.6e-296	99.61	AMSH-like ubiquitin thioesterase 3 OS=Momordica charantia OX=3673 GN=LOC11100983...	[more]
A0A5D3BT78	7.9e-259	87.38	AMSH-like ubiquitin thioesterase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3C8Z8	7.9e-259	87.38	LOW QUALITY PROTEIN: AMSH-like ubiquitin thioesterase 3 OS=Cucumis melo OX=3656 ...	[more]
A0A6J1L013	1.4e-258	88.36	AMSH-like ubiquitin thioesterase 3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A0A0KG71	3.1e-255	86.79	MPN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G311020 PE=3 SV...	[more]

BLAST of MS019325 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16144.1	1.7e-165	59.61	associated molecule with the SH3 domain of STAM 3	[more]
AT1G48790.1	1.1e-122	48.93	associated molecule with the SH3 domain of STAM 1	[more]
AT1G10600.1	2.8e-70	64.57	associated molecule with the SH3 domain of STAM 2	[more]
AT1G10600.3	4.0e-69	65.14	associated molecule with the SH3 domain of STAM 2	[more]
AT1G10600.2	3.2e-50	66.94	associated molecule with the SH3 domain of STAM 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000555	JAB1/MPN/MOV34 metalloenzyme domain	SMART	SM00232	pad1_6	coord: 332..460 e-value: 4.9E-13 score: 59.3
IPR000555	JAB1/MPN/MOV34 metalloenzyme domain	PFAM	PF01398	JAB	coord: 329..436 e-value: 6.8E-16 score: 58.3
IPR015063	USP8 dimerisation domain	PFAM	PF08969	USP8_dimer	coord: 8..95 e-value: 7.0E-11 score: 42.3
None	No IPR available	GENE3D	1.20.58.80		coord: 3..112 e-value: 7.5E-32 score: 111.8
None	No IPR available	GENE3D	3.40.140.10	Cytidine Deaminase, domain 2	coord: 317..507 e-value: 1.7E-71 score: 241.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 107..129
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 309..330
None	No IPR available	PANTHER	PTHR12947:SF18	AMSH-LIKE UBIQUITIN THIOESTERASE 3	coord: 2..507
None	No IPR available	PANTHER	PTHR12947	AMSH-LIKE PROTEASE	coord: 2..507
None	No IPR available	SUPERFAMILY	102712	JAB1/MPN domain	coord: 332..463
IPR037518	MPN domain	PROSITE	PS50249	MPN	coord: 333..463 score: 30.190849
IPR044098	STAMBP/STALP-like, MPN domain	CDD	cd08066	MPN_AMSH_like	coord: 335..507 e-value: 3.21807E-113 score: 330.326

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS019325.1	MS019325.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016579	protein deubiquitination
biological_process	GO:0070536	protein K63-linked deubiquitination
molecular_function	GO:0070122	isopeptidase activity
molecular_function	GO:0061578	Lys63-specific deubiquitinase activity
molecular_function	GO:0008237	metallopeptidase activity
molecular_function	GO:0005515	protein binding