MS019313 (gene) Bitter gourd (TR) v1

Overview
NameMS019313
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionMono-/di-acylglycerol lipase
Locationscaffold611: 294545 .. 300796 (-)
RNA-Seq ExpressionMS019313
SyntenyMS019313
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCGGGGGCAATGGCAACCTACGCGGGAGCCGCCTTGATTATTTACTATCTGCTGAGCCGGAGATTAGCATCGAAAGGCAACGACGATGATCGGAGTGGTAATATGTCCAAATCGATTAGATCGGTTAGGAGAAGGATCTCTCGGCGGCCGGCTCAAGCGCCGGCGACGTGGTTTGAGACGATTACTACTCTATCAGAGACTCTTAGGTTTACGTACGCCGAAACCTTGGGGAAATGGCCGATCGGAGATTTGGCATTCGGCATTAATTACTTGATGCGGAGGCAGGTGCGCTCCTTTTGCTTTATGATTTTTTGTTCGTTCGATTTCTCAGCCACGACTCACGATCTTTCACCGTTCGACATGCATTCGCCTGGGGCTGTTTTTTTGTTTTTGTTTTCTTTCCTTCCGTTCCTATTCGTATCGAGAGTGTAGTTTAGAGATTTTATTTCTTGAATGTGCGCATAGGACGTTGTCTTTGATTTACGAGTCTTGATATGTCGTAATTTGTTACTGTTTTAGAATTTTTGGTGCTTTTTTGCGAATGCTTGTTTTCCTTTTACAGAACTCGAAAGAGAATTTAGTCCTAAGGTCATTTATATTTTTTAATTTGGATCGGCGATATTACTCTGCCATCACTGTTCCACGTACATTCGCTTGTGGTCATTGTCTTTGGTTTTCCTCATTCCTATTCGTATCAAGAGTGTGAATCACAAAATTCCTGTTGTCGGTTTACAACATCCATTTCTTGAATGTGGATGAGGAAATTGTTTTTGATTTGAGATACTTGATATGTCGTAATTTATCGTTACTGTTTTAGATTTTTCTGTGTTTTTAGTTTGTGAATGCTTGTCATGTCCTTGTACCGGAACTCAAAAGTGAATTCAGTTTTGATGTTATGTATTTTACATATTTCGGACTTAGCGATGTTACTCTGACATGAATTACGATCGTTCAATGTTTGAGTGCATTCGCTTGTGGTTGTCGTTTTTGGTTTTCTATGCTCATGTGCGTGTCAAGAATTTGAACTACACAAAATTCCTGTGGTTGTTTAAGAGCCTCCGCCTCTCGAATGTGGATACAGGACGGTTTTTATATTTGAGAGTCATGATATGTCGTGATTAATTGTTACTGTTTTAGGTTTTTGTTGGATTTTCTGCAATTCGGATTTACTGACATTACTCTTCTTGCAGGGTAACTTACAAGTTGCTAACGTGTATGCGGGCAACGATAGTGTGCAACTTAAAGGCCCTGAAATTATCGCGGAGTTGAGTAGTTTTTTGCGGTTGCTCACCTTCTGCATGCTTTTCTCCAAGAAGCCGTTTCCAATCTTTCTAGAATCAGCCGGTTATTCTCTGGAGGACGTGCTTATTCAGAAACCAAAAGCAGGGGTATTTCACCTTTTAGAACTTGTTTAAATTTTTGCCATGATTTAGATCCATTTCAATTGTGCTCTCCTTGGGATTTATGTTTCACGGAAAGCTTGATTGTCGTTCTTAGTTATCCTCCTCAATAGGCTTAATCACTGTCTATTATCTCTTCTTAGCAATTCTAAAACAATGAAGTGCCTTCTTTACGTCGATTGGATAGCATTATCGATATTTTCGAAGAAAAATTGCATTATCAACGACATTGTAATAAAATCAGTCAGGAAAAAAAAAATGGCCCTAGTAGTTGAAGACTTGCTAATTCCACAATAATCTAAACATATGAATGTCCCCTAGATTTTATGCTTGCCTTTACTCGCAATATTTGAATATCTTCTTTTATCATCGTGTGAGTCTTTTTTTTCTCTCTTTTTTTAATCCTAACCTTTATTGTTTCCGGTGATTAGAATTCTTCATCTGGGTTGAGTTTTTGACCGTCATATTTGATTACTTCCTTTGTTAATTGCAGATTTTGAAACCTGCTTTTACAATTATTCGTGATAGTAATTCAAAATGCTTCCTTCTTCTGATTCGGGGCACTCATAGTATCAAAGATACATTAACAGCAGTAACTGGTGCAGTGGTTCCTTTTCATCATTCAGTTTTACATGATGGTGGGATAAGTAATTTAGTTTTAGGATACGCACACTTTGGGATGGTTGCTGCAGCCCGTTGGATCGCGAAGCTAAGCACTCCTTTCTTACTCAAAGCTCTCGATGATTTTCCAGACTACAAAATCAAGGTATGTTATGTTTTTTTAATTTATTTTTCTTAGTGATTGGAAGGCCTTGCTTTTGTTTGCTTTTTGCTTTTGTCTGAATATATTCATCGTTTCTTATAAAGAAAAAAGTATAACTTGAATTAATTATTATTTTTTTCTCACGCTGTCTTGTTTCCTTCTATTAACCTGACCCTCCTGTCTGATGAAAAACTTCTGTTTCGCCTACTATATACTTCACATCATAATCACGTTTTAATCAATAAATTGTTTTTTCTTAGATGTTTCCGAGATCTCTCCTTCTATTAACTGAACTCTATTGGCTGAAGAAAAACTTCTGTTTTGCCTACTATACTTCATCATAATCATGTCTTAATCAATAAATTAATCTATCTGTACATATGTAAGCTGAATTAATTTATTATTTTTCCTCTCACTGTTCTTGTTTCATTCTAGTAACCTGACTCTGCTGGCTGATGATAAACTTCTGTTTTACCTACTACCTACTTCATCATAATCAATAAATTAATTGTTCGAGATGCATGAGTCTCACAAGATAGGAAAGCTTTGTTTCCTTCTGTAATCACGTTTAATTCGACAAATTGTTCGAGATGCATGCTGTTATTCTTTGTGGCTTTTATTAGCTGTTAGATCTTTTAGTTTTGTGAAGCTTTTAATTATATATTTGACGAAAATTGGCATGTGGAGTTGATGGATATTGTGAATAACAGAAGGGATATTCATTTCTTGAAAATTAGGCACGATATTCTGTTATCATGGGATTTATCATATTAATATTTTGAACTTTTAGATAAGTGATGTTGTTTCCTATGTTATTCACCGCTAAGTCGCTGTACTGATCTGCTTCAATATATATCCAAAATCTCCTACTTAATACAAAAATCACCCTCCTTATGGGCCCACCACGAGGGGAAAGAAATCTTGGAAGAAAAATGTTGAAGCCACGAACATAATCAAACGAACTTGAAATACACTTCAATTCCACTGCTTTAACTTTGGCTAGCATCAAGGCTTTCCCTAACACCCCACCCCCATCCACCCTAGTTGGTCTTCGCCCATCATGAAAGATAAATTCATCTCGCTTGATCATCTTGGGTTGACCGTCATTAAAATTTTTACAGATAGAAAAGTGAAAGATAGAAAATAGATTTTTTTTTTTCTAATTTTTTTGGGATGACCTAGTTGTCGATAAGGGTCGTAAATAGTAAAAGACTTAGAGGGAATGTGTTCAAACATGGTGGCCACCTACTTTGGATTTAACATCCTACGAGTTTCTTTGTCAACCAAAATGTAGTAGGATCAGGCCAACGACTCGTGATTAGTCAAGGTGCGCGCAAGTTGGTCGGACACACAGATATATAGTAAGATGGGTGCAGATCAATCATGCTTTTAAGTACCTAAGTATTTTTAGTTTATGATGAGACCTTACATCCCGAGTTAGCTTGGTCAAAAGTGAAGCAAAAGATCAAGAACAATTTTTTGAACTGTTTTTCTGCTTGAAAAGAATGTTTGAATATGTATCTATTTTCCCCGGAAGCACATTTCTAATGCTTAGATTTTGATACCCAGATTGTTGGGCATTCTCTTGGTGGTGGAACCGCTGCTTTATTGACATATATTCTTCGAGAACAGAAGGAGTTCTCTTCTAGTACTTGCATCACATTTGCCCCAGGTTTGTGAATGAAAACTTCCGTTCTTTCCTTTCAAAAGATGGGCTGATTTACTGAGAAAAAAGCATGCATTCATTTCATCCATGTTTTTTCTTTAGTCATCCGAAAGTTTTCCGTTTAAAAGATGGACCGATTGACCATTTCTTAAGGAAAAAAAGAAAGAAAGACTTTTATAAAAGATAAAAATGCTATGGATTAGCAATCATGCTTGATTACATTTCATTTGCTGCATCTTCGGCTTAAGATTTGAATGGAGTTCTGATTCAGTGCGGTCGTTAACACCTTAGTTGGTTCTGAGACATGATAATTATAATAAATCAGAATAAATCGAGTTCTATTAGTTTGGATCTTATCTTCAATGATTCGTATCTTATCGCCATTTTCCTCTGTTTTCTGACGTGTATTACCATTACAACTTTATGTTCTGTTTGTGTAGCTGCGTGTATGACATGGGAGTTGGCAGAATCAGGTAAGCAGTTCATCACTACAATTATTAATGGTTCAGATCTGGTGCCCAGCTTCTCAGCTGCTTCTATAGATGACCTGCGATATGAGGTAATTAACCCATCACTGAATGGCACAGTGAAATAATATATACTTTTATATTTTTATAAAAATAACATTGTGTGATTTTTCAAATACATATATACATTTTCTTAAAAAGAAAATTTTTTTAAACAAAAAGGAAAATAAATTACTTTTATGTATTTATGGAAGTAACATTTCAAATACATATATAACAGGCAACTTGTAAGGTCCCGTTTACGGTTTTTTTTTTTTTGCCTTTGAATTATTGATTTTGAGAAATTAAAGTTTTGTTAAATAATTATTTATTTTTGGTTTATACAATAATTTCAACATTATGATTTTATTTCAAGAACTTTTCTATTCTCTCTCTAAAATCTCCCTCAGAGAGAACTCTCAAAACTCTCTCATACTTAAGTGTCAAGGACTGTAAGCTTTTAATTTTGTGATTTTTTAGTAACCTATTTAATACAAAAACACAAAATTCTCAAATTATCAGACATAGAATATTAAAAGTTTTTTTTAACTTTTTAATTTTATGTTTAGTACATGTATAAATTTTAAAATGTTTAGGAATACACAAAATTAATGACAATTAAAAGTTTAAATAATTTGTATTTTAACATACTCTCAAAATTTCCTTCTATCTGTTTTCTATTCAAATTCTCTCTCTTAAAAGGGCTCCTCTTAACTCTCCTTTTTAAGTGCCCTTTTACTTTTTCCTCTAAACTTTTACCTTCACCCTTCTCTCTCTTGCGAACTTTTTTTTTTTTTTTTTATTTTCAATCTATAGTTTGTTTCTTTCTAACTTTTATACGTCTTATCTAAAATGACACGGTTTCTACTTTTAAATTTTATTATTATTCCCAAATATGCATTTCAATCCCATATGAAGATCCTTACGATTGATGGGTATAATTAATTAGGTGACAGCATCATCGTGGCTGAATGATTTACGAGATCAGGTAGAGCGTACCAGGGTCCTCAACGTTGTTTATCGCTCTGCAAGCGCTCTCGGCTCACGCCTTCCATCTATGGCTACTGCCAAAGCTAAGGTCGCCGGTGCAGGCGCCCTTCTCCGCCCAGTCTCTAGCAGCACACAGGTTCCTTCCTTTCCTTAACTTTTTTCTATTTCTAGTTCGTTCACTCTTTAATTCTCGATTTCGCATTTGATTTTTAGGATGTCGTGAAACGTGCGGTTGTGCGAACTCGCTCCTCTCTGTCTTCCTGGTCTTGCATTGGCGCCCGCAGACGAATATCCAATCCCACCGAGTTGCCCGAAGCTCCTCTCATAGCTGAAGAAGTTACTATTAATGCAACTGAAAAGAAGAAGCTACGCTCTGGTTCTTCAAGTGATGATAGTTCAGATCATGACACAGACGAGGAACGGCACCTTATTCATGAAGAAAGAATCGTTACCTCAACCGACGTCGAAGACATTACGGATGGTGAGTTGTGGTATGAACTGGAGAAGGAACTTCAGCGGCAAGAGAAAAAAGTCGATGTTATTACCCGAGAAGTTGGTGCCGCTGCAGCTAAAGAAATCGAAGAAGAAGAAGAAGAAGAAGAAGAGAGATTGCTTACAGATGCTGAGGGAAGCAGTGAGAAGCCATTGTCTTCTCTGGATGCTTCGGAAAACATACGTTTCTATCCTCCCGGCAGAACCATGCATATTGTTTCAGTGCTCTCGCCGGATTCTGATAATTTAGCTCACGATGAGGAGATTGTGCAGGAAAAAGTTGGTATATATGAGACGCCTAGAGAATTGTACAGTAAGCTGCGTCTCTCTAGAACAATGATTAATGATCATTATATGCCCATGTACAAAAAGATGATGGAATTGTTGATCAAGGAACTTGAAGAAGATGTATCAAGTAGTTACGAATTG

mRNA sequence

ATGGCGGCGGGGGCAATGGCAACCTACGCGGGAGCCGCCTTGATTATTTACTATCTGCTGAGCCGGAGATTAGCATCGAAAGGCAACGACGATGATCGGAGTGGTAATATGTCCAAATCGATTAGATCGGTTAGGAGAAGGATCTCTCGGCGGCCGGCTCAAGCGCCGGCGACGTGGTTTGAGACGATTACTACTCTATCAGAGACTCTTAGGTTTACGTACGCCGAAACCTTGGGGAAATGGCCGATCGGAGATTTGGCATTCGGCATTAATTACTTGATGCGGAGGCAGGGTAACTTACAAGTTGCTAACGTGTATGCGGGCAACGATAGTGTGCAACTTAAAGGCCCTGAAATTATCGCGGAGTTGAGTAGTTTTTTGCGGTTGCTCACCTTCTGCATGCTTTTCTCCAAGAAGCCGTTTCCAATCTTTCTAGAATCAGCCGGTTATTCTCTGGAGGACGTGCTTATTCAGAAACCAAAAGCAGGGATTTTGAAACCTGCTTTTACAATTATTCGTGATAGTAATTCAAAATGCTTCCTTCTTCTGATTCGGGGCACTCATAGTATCAAAGATACATTAACAGCAGTAACTGGTGCAGTGGTTCCTTTTCATCATTCAGTTTTACATGATGGTGGGATAAGTAATTTAGTTTTAGGATACGCACACTTTGGGATGGTTGCTGCAGCCCGTTGGATCGCGAAGCTAAGCACTCCTTTCTTACTCAAAGCTCTCGATGATTTTCCAGACTACAAAATCAAGATTGTTGGGCATTCTCTTGGTGGTGGAACCGCTGCTTTATTGACATATATTCTTCGAGAACAGAAGGAGTTCTCTTCTAGTACTTGCATCACATTTGCCCCAGCTGCGTGTATGACATGGGAGTTGGCAGAATCAGGTAAGCAGTTCATCACTACAATTATTAATGGTTCAGATCTGGTGCCCAGCTTCTCAGCTGCTTCTATAGATGACCTGCGATATGAGGTGACAGCATCATCGTGGCTGAATGATTTACGAGATCAGGTAGAGCGTACCAGGGTCCTCAACGTTGTTTATCGCTCTGCAAGCGCTCTCGGCTCACGCCTTCCATCTATGGCTACTGCCAAAGCTAAGGTCGCCGGTGCAGGCGCCCTTCTCCGCCCAGTCTCTAGCAGCACACAGGATGTCGTGAAACGTGCGGTTGTGCGAACTCGCTCCTCTCTGTCTTCCTGGTCTTGCATTGGCGCCCGCAGACGAATATCCAATCCCACCGAGTTGCCCGAAGCTCCTCTCATAGCTGAAGAAGTTACTATTAATGCAACTGAAAAGAAGAAGCTACGCTCTGGTTCTTCAAGTGATGATAGTTCAGATCATGACACAGACGAGGAACGGCACCTTATTCATGAAGAAAGAATCGTTACCTCAACCGACGTCGAAGACATTACGGATGGTGAGTTGTGGTATGAACTGGAGAAGGAACTTCAGCGGCAAGAGAAAAAAGTCGATGTTATTACCCGAGAAGTTGGTGCCGCTGCAGCTAAAGAAATCGAAGAAGAAGAAGAAGAAGAAGAAGAGAGATTGCTTACAGATGCTGAGGGAAGCAGTGAGAAGCCATTGTCTTCTCTGGATGCTTCGGAAAACATACGTTTCTATCCTCCCGGCAGAACCATGCATATTGTTTCAGTGCTCTCGCCGGATTCTGATAATTTAGCTCACGATGAGGAGATTGTGCAGGAAAAAGTTGGTATATATGAGACGCCTAGAGAATTGTACAGTAAGCTGCGTCTCTCTAGAACAATGATTAATGATCATTATATGCCCATGTACAAAAAGATGATGGAATTGTTGATCAAGGAACTTGAAGAAGATGTATCAAGTAGTTACGAATTG

Coding sequence (CDS)

ATGGCGGCGGGGGCAATGGCAACCTACGCGGGAGCCGCCTTGATTATTTACTATCTGCTGAGCCGGAGATTAGCATCGAAAGGCAACGACGATGATCGGAGTGGTAATATGTCCAAATCGATTAGATCGGTTAGGAGAAGGATCTCTCGGCGGCCGGCTCAAGCGCCGGCGACGTGGTTTGAGACGATTACTACTCTATCAGAGACTCTTAGGTTTACGTACGCCGAAACCTTGGGGAAATGGCCGATCGGAGATTTGGCATTCGGCATTAATTACTTGATGCGGAGGCAGGGTAACTTACAAGTTGCTAACGTGTATGCGGGCAACGATAGTGTGCAACTTAAAGGCCCTGAAATTATCGCGGAGTTGAGTAGTTTTTTGCGGTTGCTCACCTTCTGCATGCTTTTCTCCAAGAAGCCGTTTCCAATCTTTCTAGAATCAGCCGGTTATTCTCTGGAGGACGTGCTTATTCAGAAACCAAAAGCAGGGATTTTGAAACCTGCTTTTACAATTATTCGTGATAGTAATTCAAAATGCTTCCTTCTTCTGATTCGGGGCACTCATAGTATCAAAGATACATTAACAGCAGTAACTGGTGCAGTGGTTCCTTTTCATCATTCAGTTTTACATGATGGTGGGATAAGTAATTTAGTTTTAGGATACGCACACTTTGGGATGGTTGCTGCAGCCCGTTGGATCGCGAAGCTAAGCACTCCTTTCTTACTCAAAGCTCTCGATGATTTTCCAGACTACAAAATCAAGATTGTTGGGCATTCTCTTGGTGGTGGAACCGCTGCTTTATTGACATATATTCTTCGAGAACAGAAGGAGTTCTCTTCTAGTACTTGCATCACATTTGCCCCAGCTGCGTGTATGACATGGGAGTTGGCAGAATCAGGTAAGCAGTTCATCACTACAATTATTAATGGTTCAGATCTGGTGCCCAGCTTCTCAGCTGCTTCTATAGATGACCTGCGATATGAGGTGACAGCATCATCGTGGCTGAATGATTTACGAGATCAGGTAGAGCGTACCAGGGTCCTCAACGTTGTTTATCGCTCTGCAAGCGCTCTCGGCTCACGCCTTCCATCTATGGCTACTGCCAAAGCTAAGGTCGCCGGTGCAGGCGCCCTTCTCCGCCCAGTCTCTAGCAGCACACAGGATGTCGTGAAACGTGCGGTTGTGCGAACTCGCTCCTCTCTGTCTTCCTGGTCTTGCATTGGCGCCCGCAGACGAATATCCAATCCCACCGAGTTGCCCGAAGCTCCTCTCATAGCTGAAGAAGTTACTATTAATGCAACTGAAAAGAAGAAGCTACGCTCTGGTTCTTCAAGTGATGATAGTTCAGATCATGACACAGACGAGGAACGGCACCTTATTCATGAAGAAAGAATCGTTACCTCAACCGACGTCGAAGACATTACGGATGGTGAGTTGTGGTATGAACTGGAGAAGGAACTTCAGCGGCAAGAGAAAAAAGTCGATGTTATTACCCGAGAAGTTGGTGCCGCTGCAGCTAAAGAAATCGAAGAAGAAGAAGAAGAAGAAGAAGAGAGATTGCTTACAGATGCTGAGGGAAGCAGTGAGAAGCCATTGTCTTCTCTGGATGCTTCGGAAAACATACGTTTCTATCCTCCCGGCAGAACCATGCATATTGTTTCAGTGCTCTCGCCGGATTCTGATAATTTAGCTCACGATGAGGAGATTGTGCAGGAAAAAGTTGGTATATATGAGACGCCTAGAGAATTGTACAGTAAGCTGCGTCTCTCTAGAACAATGATTAATGATCATTATATGCCCATGTACAAAAAGATGATGGAATTGTTGATCAAGGAACTTGAAGAAGATGTATCAAGTAGTTACGAATTG

Protein sequence

MAAGAMATYAGAALIIYYLLSRRLASKGNDDDRSGNMSKSIRSVRRRISRRPAQAPATWFETITTLSETLRFTYAETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEIIAELSSFLRLLTFCMLFSKKPFPIFLESAGYSLEDVLIQKPKAGILKPAFTIIRDSNSKCFLLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPFLLKALDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESGKQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASALGSRLPSMATAKAKVAGAGALLRPVSSSTQDVVKRAVVRTRSSLSSWSCIGARRRISNPTELPEAPLIAEEVTINATEKKKLRSGSSSDDSSDHDTDEERHLIHEERIVTSTDVEDITDGELWYELEKELQRQEKKVDVITREVGAAAAKEIEEEEEEEEERLLTDAEGSSEKPLSSLDASENIRFYPPGRTMHIVSVLSPDSDNLAHDEEIVQEKVGIYETPRELYSKLRLSRTMINDHYMPMYKKMMELLIKELEEDVSSSYEL
Homology
BLAST of MS019313 vs. NCBI nr
Match: XP_022138525.1 (uncharacterized protein LOC111009670 [Momordica charantia])

HSP 1 Score: 1173.7 bits (3035), Expect = 0.0e+00
Identity = 620/624 (99.36%), Postives = 620/624 (99.36%), Query Frame = 0

Query: 1   MAAGAMATYAGAALIIYYLLSRRLASKGNDDDRSGNMSKSIRSVRRRISRRPAQAPATWF 60
           MAAGAMATYAGAALIIYYLLSRRLASKGNDDDRSGNMSKSIRSVRRRISRRPAQAPATWF
Sbjct: 1   MAAGAMATYAGAALIIYYLLSRRLASKGNDDDRSGNMSKSIRSVRRRISRRPAQAPATWF 60

Query: 61  ETITTLSETLRFTYAETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII 120
           ETITTLSETLRFTYAETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII
Sbjct: 61  ETITTLSETLRFTYAETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII 120

Query: 121 AELSSFLRLLTFCMLFSKKPFPIFLESAGYSLEDVLIQKPKAGILKPAFTIIRDSNSKCF 180
           AELSSFLRLLTFCMLFSKKPFPIFLESAGYSLEDVLIQKPKAGILKPAFTIIRDSNSKCF
Sbjct: 121 AELSSFLRLLTFCMLFSKKPFPIFLESAGYSLEDVLIQKPKAGILKPAFTIIRDSNSKCF 180

Query: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240
           LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF
Sbjct: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240

Query: 241 LLKALDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300
           LLKALDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG
Sbjct: 241 LLKALDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300

Query: 301 KQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360
           KQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASALGS
Sbjct: 301 KQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360

Query: 361 RLPSMATAKAKVAGAGALLRPVSSSTQDVVKRAVVRTRSSLSSWSCIGARRRISNPT-EL 420
           RLPSMATAKAKVAGAGALLRPVSSSTQDVVKRAVVRTRSSLSSWSCIGARRRISNPT EL
Sbjct: 361 RLPSMATAKAKVAGAGALLRPVSSSTQDVVKRAVVRTRSSLSSWSCIGARRRISNPTEEL 420

Query: 421 PEAPLIAEEVTINATEKKKLRSGSSSDDSSDHDTDEERHLIHEERIVTSTDVEDITDGEL 480
           PEAPLIAEEVTINATEKKKLRSGSSSDDSSDHDTDEERHLIHEERIVTSTDVEDITDGEL
Sbjct: 421 PEAPLIAEEVTINATEKKKLRSGSSSDDSSDHDTDEERHLIHEERIVTSTDVEDITDGEL 480

Query: 481 WYELEKELQRQEKKVDVITREVGAAAAKEIEEEEEEEEERLLTDAEGSSEKPLSSLDASE 540
           WYELEKELQRQEKKVDVITREVGAAAAKEI  EEEEEEERLLTDAEGSSEKPLSSLDASE
Sbjct: 481 WYELEKELQRQEKKVDVITREVGAAAAKEI--EEEEEEERLLTDAEGSSEKPLSSLDASE 540

Query: 541 NIRFYPPGRTMHIVSVLSPDSDNLAHDEEIVQEKVGIYETPRELYSKLRLSRTMINDHYM 600
           NIRFYPPGRTMHIVSVLSPDSDNLAH EEIVQEKVGIYETPRELYSKLRLSRTMINDHYM
Sbjct: 541 NIRFYPPGRTMHIVSVLSPDSDNLAHAEEIVQEKVGIYETPRELYSKLRLSRTMINDHYM 600

Query: 601 PMYKKMMELLIKELEEDVSSSYEL 624
           PMYKKMMELLIKELEEDVSSSYEL
Sbjct: 601 PMYKKMMELLIKELEEDVSSSYEL 622

BLAST of MS019313 vs. NCBI nr
Match: XP_038874736.1 (uncharacterized protein LOC120067274 [Benincasa hispida])

HSP 1 Score: 1041.2 bits (2691), Expect = 3.6e-300
Identity = 563/642 (87.69%), Postives = 586/642 (91.28%), Query Frame = 0

Query: 1   MAAGAMATYAGAALIIYYLLSRRLASKGNDDDRSGNMSKSIRSVRRRISRRPAQAPATWF 60
           MAAGAMATYAGAALIIYYLLSRRLA+KG++DDRS N+SKSIRS RRRISRRPAQAPATWF
Sbjct: 1   MAAGAMATYAGAALIIYYLLSRRLAAKGDEDDRSDNLSKSIRSGRRRISRRPAQAPATWF 60

Query: 61  ETITTLSETLRFTYAETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII 120
           ETITTLSETLRFTY+ETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII
Sbjct: 61  ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII 120

Query: 121 AELSSFLRLLTFCMLFSKKPFPIFLESAGYSLEDVLIQKPKAGILKPAFTIIRDSNSKCF 180
            EL SFLRLLTFCMLFSKKPFPIFLESAGYS E+VLIQKPKAGILKPAFTIIRDSNSKCF
Sbjct: 121 TELKSFLRLLTFCMLFSKKPFPIFLESAGYSPEEVLIQKPKAGILKPAFTIIRDSNSKCF 180

Query: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240
           LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF
Sbjct: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240

Query: 241 LLKALDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300
           LLK LDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG
Sbjct: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300

Query: 301 KQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360
           KQFITTIINGSDLVPSFSAASIDDLR EVTASSWLNDLRDQVERTRVLNVVYRSASALGS
Sbjct: 301 KQFITTIINGSDLVPSFSAASIDDLRSEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360

Query: 361 RLPSMATAKAKVAGAGALLRPVSSSTQDVVKRAVVRTRSSLSSWSCIGARRR----ISNP 420
           RLPS+ATAKAKVAGAGALLRPVS++TQ  VK AVVRTRSSLSSWSC+GARRR    +SNP
Sbjct: 361 RLPSIATAKAKVAGAGALLRPVSTTTQAAVKSAVVRTRSSLSSWSCMGARRRNGGILSNP 420

Query: 421 T-ELPEAPLIAE---------EVTINATEKKKL-RSGSSSDDSSDHDTDEER-HLIHEER 480
           T ELPE PLI E         EVTINA EKKK   SG+S DDSSD DTDEER HLI EER
Sbjct: 421 TEELPEVPLITERNHESLLAGEVTINAIEKKKKPESGTSGDDSSDRDTDEERHHLITEER 480

Query: 481 IVTSTDVEDITDGELWYELEKELQRQEKKVDVITREVG-AAAAKEIEEEEEEEEERLLTD 540
           ++ STDVEDITDGELWYELEKELQRQEKKVDV TRE   A  AKEI+EEEE     +LTD
Sbjct: 481 MIASTDVEDITDGELWYELEKELQRQEKKVDVTTREADVATVAKEIKEEEES----MLTD 540

Query: 541 AEGSSEKPLSSLDASENIRFYPPGRTMHIVSVLSPDSDNLAH--DEEIVQEKVGIYETPR 600
            EGSSEKPLSSLDASENIRFYPPG+TMHIVS+ S DSDNL    DEEI+QE+VGIYETPR
Sbjct: 541 VEGSSEKPLSSLDASENIRFYPPGKTMHIVSIPSSDSDNLVQDDDEEIMQERVGIYETPR 600

Query: 601 ELYSKLRLSRTMINDHYMPMYKKMMELLIKELEEDVSSSYEL 624
           ELYSKLRLSRTMINDHYMPMYKKMME LI ELE+DV S+YE+
Sbjct: 601 ELYSKLRLSRTMINDHYMPMYKKMMESLINELEKDVLSNYEM 638

BLAST of MS019313 vs. NCBI nr
Match: XP_008458404.1 (PREDICTED: uncharacterized protein LOC103497826 [Cucumis melo] >TYK02921.1 Sn1-specific diacylglycerol lipase alpha [Cucumis melo var. makuwa])

HSP 1 Score: 1031.9 bits (2667), Expect = 2.2e-297
Identity = 560/643 (87.09%), Postives = 586/643 (91.14%), Query Frame = 0

Query: 1   MAAGAMATYAGAALIIYYLLSRRLASKGNDDDRSGNMSKSIRSVRRRISRRPAQAPATWF 60
           MAAGAMATYAGAALIIYYLLSRRLA+KG++DDRSGN+SKSIRS RRRISRRPAQAPATWF
Sbjct: 1   MAAGAMATYAGAALIIYYLLSRRLAAKGDEDDRSGNLSKSIRSGRRRISRRPAQAPATWF 60

Query: 61  ETITTLSETLRFTYAETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII 120
           ETITTLSETLRFTY+ETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII
Sbjct: 61  ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII 120

Query: 121 AELSSFLRLLTFCMLFSKKPFPIFLESAGYSLEDVLIQKPKAGILKPAFTIIRDSNSKCF 180
           AEL SFLRLLTFCMLFSKKPFPIFLESAGYS E+VLIQKPKAGILKPAFTIIRDS+SKCF
Sbjct: 121 AELKSFLRLLTFCMLFSKKPFPIFLESAGYSQEEVLIQKPKAGILKPAFTIIRDSSSKCF 180

Query: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240
           LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF
Sbjct: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240

Query: 241 LLKALDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300
           LLK LDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG
Sbjct: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300

Query: 301 KQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360
           KQFITTIINGSDLVPSFSAASIDDLR EVTASSWLNDLRDQVERTRVLNVVYRSASALGS
Sbjct: 301 KQFITTIINGSDLVPSFSAASIDDLRSEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360

Query: 361 RLPSMATAKAKVAGAGALLRPVSSSTQDVVKRAVVRTRSSLSSWSCIGARRR----ISNP 420
           RLPS+ATAKAKVAGAGALLRPVS++TQ  VK AVVRTRSSLSSWSC+GARRR    +SNP
Sbjct: 361 RLPSIATAKAKVAGAGALLRPVSTTTQAAVKSAVVRTRSSLSSWSCMGARRRNGGILSNP 420

Query: 421 T-ELPEAP---------LIAEEVTINATE-KKKLRSGSSS-DDSSDHDTDEER-HLIHEE 480
           T ELPE P         LI+EEVTIN  E KKK  SGSSS DD+SDHDTDEER HLI EE
Sbjct: 421 TEELPEVPRITERNHESLISEEVTINGIEKKKKTESGSSSCDDNSDHDTDEERHHLITEE 480

Query: 481 RIVTSTDVEDITDGELWYELEKELQRQEKKVDVITRE-VGAAAAKEIEEEEEEEEERLLT 540
           RI+ STDVEDITDGELWYELEKELQRQE+KVD  TRE   A  AKEI+EEEE     +LT
Sbjct: 481 RIIASTDVEDITDGELWYELEKELQRQERKVDANTREGKVATVAKEIKEEEES----MLT 540

Query: 541 DAEGSSEKPLSSLDASENIRFYPPGRTMHIVSVLSPDSDNLAHD--EEIVQEKVGIYETP 600
           D EGSSEKPLSSLDASEN+RFYPPG+TMHIVS  SP+SDNL  D  +E +QE VGIYETP
Sbjct: 541 DVEGSSEKPLSSLDASENVRFYPPGKTMHIVSTPSPNSDNLVQDDEDESMQEIVGIYETP 600

Query: 601 RELYSKLRLSRTMINDHYMPMYKKMMELLIKELEEDVSSSYEL 624
           RELYSKLRLSRTMINDHYMPMYKKMME LI +LE DV S+YE+
Sbjct: 601 RELYSKLRLSRTMINDHYMPMYKKMMESLINQLENDVISNYEM 639

BLAST of MS019313 vs. NCBI nr
Match: XP_004150086.1 (uncharacterized protein LOC101210872 [Cucumis sativus] >KGN47411.1 hypothetical protein Csa_022986 [Cucumis sativus])

HSP 1 Score: 1021.9 bits (2641), Expect = 2.3e-294
Identity = 551/641 (85.96%), Postives = 578/641 (90.17%), Query Frame = 0

Query: 1   MAAGAMATYAGAALIIYYLLSRRLASKGNDDDRSGNMSKSIRSVRRRISRRPAQAPATWF 60
           MAAGAMATYAGAALIIYY LSRRLA+KG++DDRSGN+SKSIRS RRRISRRPAQAPATWF
Sbjct: 1   MAAGAMATYAGAALIIYYFLSRRLAAKGDEDDRSGNLSKSIRSCRRRISRRPAQAPATWF 60

Query: 61  ETITTLSETLRFTYAETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII 120
           ETITTLSETLRFTY+ETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII
Sbjct: 61  ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII 120

Query: 121 AELSSFLRLLTFCMLFSKKPFPIFLESAGYSLEDVLIQKPKAGILKPAFTIIRDSNSKCF 180
           AEL SFLRLLTFCMLFSKKPFPIFLESAGYS E+VLIQKPKAGILKPAFTIIRDS+SKCF
Sbjct: 121 AELKSFLRLLTFCMLFSKKPFPIFLESAGYSQEEVLIQKPKAGILKPAFTIIRDSSSKCF 180

Query: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240
           LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF
Sbjct: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240

Query: 241 LLKALDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300
           LLK LDDFPDYKIKIVGHSLGGGTAALLTYILREQ EFSSSTCITFAPAACMTWELAESG
Sbjct: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQTEFSSSTCITFAPAACMTWELAESG 300

Query: 301 KQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360
           KQFITTIINGSDLVPSFSAASIDDLR EVTASSWLNDLRDQVERTRVLNVVYRSASALGS
Sbjct: 301 KQFITTIINGSDLVPSFSAASIDDLRSEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360

Query: 361 RLPSMATAKAKVAGAGALLRPVSSSTQDVVKRAVVRTRSSLSSWSCIGARRR----ISNP 420
           RLPS+ATAKAKVAGAGALLRPVS++TQ  VK AVVRTRSSLSSWSC+GARRR    +SNP
Sbjct: 361 RLPSIATAKAKVAGAGALLRPVSTTTQAAVKSAVVRTRSSLSSWSCMGARRRNGNILSNP 420

Query: 421 T-ELPEAPLI---------AEEVTINATEKKKL-RSGSSSDDSSDHDTDEER-HLIHEER 480
           T ELPE PL+          EEV IN  EKKK    GSS DDSSDHDTDEE+ H+I  ER
Sbjct: 421 TEELPEVPLMTERNHESLKCEEVRINGIEKKKKPEFGSSCDDSSDHDTDEEQHHIITGER 480

Query: 481 IVTSTDVEDITDGELWYELEKELQRQEKKVDVITREVGAAAAKEIEEEEEEEEERLLTDA 540
           I+ STDVEDITDGELWYELEKELQRQEKKVD  TRE   A    + +E +EEEE +LTD 
Sbjct: 481 IIASTDVEDITDGELWYELEKELQRQEKKVDANTREAKVAT---VGKEIKEEEESMLTDV 540

Query: 541 EGSSEKPLSSLDASENIRFYPPGRTMHIVSVLSPDSDNLAHD--EEIVQEKVGIYETPRE 600
           EGSSEKPLSSLDASENIRFYPPG+TMHIVS  SP+SDNL  D  +E  QE VGIYETPRE
Sbjct: 541 EGSSEKPLSSLDASENIRFYPPGKTMHIVSTPSPNSDNLVQDDEDESTQEIVGIYETPRE 600

Query: 601 LYSKLRLSRTMINDHYMPMYKKMMELLIKELEEDVSSSYEL 624
           LYSKLRLSRTMINDHYMPMYKKMME LI +LE+DV S+YE+
Sbjct: 601 LYSKLRLSRTMINDHYMPMYKKMMESLINQLEKDVISNYEM 638

BLAST of MS019313 vs. NCBI nr
Match: KAG6575088.1 (Sn1-specific diacylglycerol lipase alpha, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1005.7 bits (2599), Expect = 1.7e-289
Identity = 552/652 (84.66%), Postives = 586/652 (89.88%), Query Frame = 0

Query: 1   MAAGAMATYAGAALIIYYLLSRRLASKGNDDDRSGNMSKSIRSVRRRISRRPAQAPATWF 60
           MAAGA+ATYAGAAL++YYLLSRRLA+KG++DDRS N+S+SIRS RRRISRRPAQAPATWF
Sbjct: 1   MAAGALATYAGAALLMYYLLSRRLAAKGDEDDRSDNLSESIRSGRRRISRRPAQAPATWF 60

Query: 61  ETITTLSETLRFTYAETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII 120
           ETITTLSETLRFTYAETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQL+GPEII
Sbjct: 61  ETITTLSETLRFTYAETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLRGPEII 120

Query: 121 AELSSFLRLLTFCMLFSKKPFPIFLESAGYSLEDVLIQKPKAGILKPAFTIIRDSNSKCF 180
           AEL SFLRLLTFCMLFSKKPFPIFLESAGYS EDVLIQKPKAGILKPAFTIIRDSNSKCF
Sbjct: 121 AELKSFLRLLTFCMLFSKKPFPIFLESAGYSQEDVLIQKPKAGILKPAFTIIRDSNSKCF 180

Query: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240
           LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTP 
Sbjct: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPR 240

Query: 241 LLKALDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300
           LLK LDDFPDYKIKIVGHSLGGGTAALLTYILRE+K+FSS+TCITFAPAACMTWELAESG
Sbjct: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILRERKDFSSTTCITFAPAACMTWELAESG 300

Query: 301 KQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360
           KQFITTIIN SDLVPSFSAASIDDLR EVTASSWLNDLRDQVERTRVL+VVYRSASALGS
Sbjct: 301 KQFITTIINSSDLVPSFSAASIDDLRSEVTASSWLNDLRDQVERTRVLSVVYRSASALGS 360

Query: 361 RLPSMATAKAKVAGAGALLRPVSSSTQDVVKRAVVRTRSSLSSWSCIGARRR----ISNP 420
           RLPS+ATAKAKVAGAGALLRPVS++TQ  VK AVVRTRSSL+SWSC+GARRR    + NP
Sbjct: 361 RLPSIATAKAKVAGAGALLRPVSTTTQTAVKSAVVRTRSSLASWSCMGARRRNGGLLPNP 420

Query: 421 T-ELPEAPLIAE----------EVTINAT--EKKKLRSGSSS---DDSSDHDTDEER-HL 480
           T ELPEAPL  E          EVT N T  +KKKL SGSSS   DDSSDHDTD+ER HL
Sbjct: 421 TEELPEAPLRTERKHESLIADDEVTTNNTILKKKKLESGSSSLGDDDSSDHDTDDERHHL 480

Query: 481 IHEER-IVTSTDVEDITDGELWYELEKELQRQEKK----VDVITREVG-AAAAKEIEEEE 540
           I+E++ + +STDV+DITDGELWYELEKELQRQEKK     DVITRE   AA AKEI EEE
Sbjct: 481 INEDKTVASSTDVDDITDGELWYELEKELQRQEKKTKTTTDVITREANVAAVAKEIVEEE 540

Query: 541 EEEEERLLTDAEGSSEKPLSSLDASENIRFYPPGRTMHIVSVLSPDSDN-LAHDEEIVQE 600
           E     +LT+ EGSSEKPLSSLDASENIRFYPPG+TMHIVSV S DSDN   HD EIVQE
Sbjct: 541 ES----MLTEVEGSSEKPLSSLDASENIRFYPPGKTMHIVSVPSSDSDNSFVHDNEIVQE 600

Query: 601 KVGIYETPRELYSKLRLSRTMINDHYMPMYKKMMELLIKELEEDVSSS-YEL 624
           +VGIYETPR LYSKLRLSRTMINDHYMPMYKKM+ELLI +LE DVSSS YE+
Sbjct: 601 RVGIYETPRGLYSKLRLSRTMINDHYMPMYKKMIELLINQLERDVSSSNYEM 648

BLAST of MS019313 vs. ExPASy Swiss-Prot
Match: P0C1S9 (Diacylglycerol lipase-beta OS=Rattus norvegicus OX=10116 GN=Daglb PE=1 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 2.8e-13
Identity = 53/192 (27.60%), Postives = 97/192 (50.52%), Query Frame = 0

Query: 141 FPIFLESAGYSLEDVLIQKPKAGILKPAFTIIRDSNSKCFLLLIRGTHSIKDTLTAVTGA 200
           F   L++ G    D +       + +  F ++ D   +  ++ +RGT S++D LT ++  
Sbjct: 330 FASILKTTGLQYRDFIHVSFHDKVYELPFIVVLDHRKESVVVAVRGTMSLQDVLTDLSAE 389

Query: 201 VVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAK--LSTPFLLKALDDFPDYKIKIVGH 260
                 ++  D  + + V   AH G+  AAR+I +  ++   L +A    P+Y++ +VGH
Sbjct: 390 ----SENLELDIELQDCV---AHKGIAQAARYIYRRLVNDGILSQAFSVAPEYRLVVVGH 449

Query: 261 SLGGGTAALLTYILREQKEFSSSTCITFA-PAACMTWELAESGKQFITTIINGSDLVPSF 320
           SLG G AALL  +LR    +       F+ P   ++  L E  K F+ ++I G D++P  
Sbjct: 450 SLGAGAAALLAIMLR--GAYPQVRAYAFSPPRGLLSKSLFEYSKDFVVSLILGMDVIPRL 509

Query: 321 SAASIDDLRYEV 330
           S A+++DL+  +
Sbjct: 510 SVANMEDLKRRI 512

BLAST of MS019313 vs. ExPASy Swiss-Prot
Match: Q9Y4D2 (Diacylglycerol lipase-alpha OS=Homo sapiens OX=9606 GN=DAGLA PE=1 SV=3)

HSP 1 Score: 77.0 bits (188), Expect = 8.3e-13
Identity = 55/188 (29.26%), Postives = 88/188 (46.81%), Query Frame = 0

Query: 154 DVLIQKPKAGILKPAFTIIRDSNSKCFLLLIRGTHSIKDTLTAVTGAV----VPFHHSVL 213
           D++       + +  F +  D + K  ++ IRGT S KD LT +TG      V  HH   
Sbjct: 367 DIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGT- 426

Query: 214 HDGGISNLVLGYAHFGMVAAARWIAK-------LSTPFLLKALDDFPDYKIKIVGHSLGG 273
                        H GMV +A +I K       LS  F          Y + +VGHSLG 
Sbjct: 427 ----------WLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGA 486

Query: 274 GTAALLTYILREQKEFSSSTCITFA-PAACMTWELAESGKQFITTIINGSDLVPSFSAAS 330
           GTAA+L+++LR Q  + +  C  ++ P   ++ +  E  K+F+T ++ G DLVP    + 
Sbjct: 487 GTAAILSFLLRPQ--YPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQ 541

BLAST of MS019313 vs. ExPASy Swiss-Prot
Match: Q6WQJ1 (Diacylglycerol lipase-alpha OS=Mus musculus OX=10090 GN=Dagla PE=1 SV=2)

HSP 1 Score: 76.6 bits (187), Expect = 1.1e-12
Identity = 56/184 (30.43%), Postives = 89/184 (48.37%), Query Frame = 0

Query: 154 DVLIQKPKAGILKPAFTIIRDSNSKCFLLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGG 213
           D++       + +  F +  D + K  ++ IRGT S KD LT +TG         L   G
Sbjct: 367 DIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDA-----ERLPVEG 426

Query: 214 ISNLVLGYAHFGMVAAARWIAK-------LSTPFLLKALDDFPDYKIKIVGHSLGGGTAA 273
                LG  H GMV +A +I K       LS  F          Y + +VGHSLG GTAA
Sbjct: 427 HRGTWLG--HKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAA 486

Query: 274 LLTYILREQKEFSSSTCITFA-PAACMTWELAESGKQFITTIINGSDLVPSFSAASIDDL 330
           +L+++LR Q  + +  C  ++ P   ++ +  E  K+F+T ++ G DLVP    + ++  
Sbjct: 487 ILSFLLRPQ--YPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGF 541

BLAST of MS019313 vs. ExPASy Swiss-Prot
Match: Q5YLM1 (Diacylglycerol lipase-alpha OS=Rattus norvegicus OX=10116 GN=Dagla PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 1.1e-12
Identity = 56/184 (30.43%), Postives = 89/184 (48.37%), Query Frame = 0

Query: 154 DVLIQKPKAGILKPAFTIIRDSNSKCFLLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGG 213
           D++       + +  F +  D + K  ++ IRGT S KD LT +TG         L   G
Sbjct: 367 DIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDA-----ERLPVEG 426

Query: 214 ISNLVLGYAHFGMVAAARWIAK-------LSTPFLLKALDDFPDYKIKIVGHSLGGGTAA 273
                LG  H GMV +A +I K       LS  F          Y + +VGHSLG GTAA
Sbjct: 427 HRGTWLG--HKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAA 486

Query: 274 LLTYILREQKEFSSSTCITFA-PAACMTWELAESGKQFITTIINGSDLVPSFSAASIDDL 330
           +L+++LR Q  + +  C  ++ P   ++ +  E  K+F+T ++ G DLVP    + ++  
Sbjct: 487 ILSFLLRPQ--YPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGF 541

BLAST of MS019313 vs. ExPASy Swiss-Prot
Match: Q8NCG7 (Diacylglycerol lipase-beta OS=Homo sapiens OX=9606 GN=DAGLB PE=1 SV=2)

HSP 1 Score: 75.5 bits (184), Expect = 2.4e-12
Identity = 54/194 (27.84%), Postives = 94/194 (48.45%), Query Frame = 0

Query: 141 FPIFLESAGYSLEDVLIQKPKAGILKPAFTIIRDSNSKCFLLLIRGTHSIKDTLTAVTG- 200
           F   L + G    D +       + +  F +  D   +  ++ +RGT S++D LT ++  
Sbjct: 330 FGSILHTTGLQYRDFIHVSFHDKVYELPFLVALDHRKESVVVAVRGTMSLQDVLTDLSAE 389

Query: 201 AVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAK--LSTPFLLKALDDFPDYKIKIVG 260
           + V      + D          AH G+  AAR++ +  ++   L +A    P+Y++ IVG
Sbjct: 390 SEVLDVECEVQD--------RLAHKGISQAARYVYQRLINDGILSQAFSIAPEYRLVIVG 449

Query: 261 HSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWE--LAESGKQFITTIINGSDLVP 320
           HSLGGG AALL  +LR    +    C  F+P   + W   L E  + FI +++ G D++P
Sbjct: 450 HSLGGGAAALLATMLR--AAYPQVRCYAFSPPRGL-WSKALQEYSQSFIVSLVLGKDVIP 509

Query: 321 SFSAASIDDLRYEV 330
             S  +++DL+  +
Sbjct: 510 RLSVTNLEDLKRRI 512

BLAST of MS019313 vs. ExPASy TrEMBL
Match: A0A6J1C9P1 (uncharacterized protein LOC111009670 OS=Momordica charantia OX=3673 GN=LOC111009670 PE=4 SV=1)

HSP 1 Score: 1173.7 bits (3035), Expect = 0.0e+00
Identity = 620/624 (99.36%), Postives = 620/624 (99.36%), Query Frame = 0

Query: 1   MAAGAMATYAGAALIIYYLLSRRLASKGNDDDRSGNMSKSIRSVRRRISRRPAQAPATWF 60
           MAAGAMATYAGAALIIYYLLSRRLASKGNDDDRSGNMSKSIRSVRRRISRRPAQAPATWF
Sbjct: 1   MAAGAMATYAGAALIIYYLLSRRLASKGNDDDRSGNMSKSIRSVRRRISRRPAQAPATWF 60

Query: 61  ETITTLSETLRFTYAETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII 120
           ETITTLSETLRFTYAETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII
Sbjct: 61  ETITTLSETLRFTYAETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII 120

Query: 121 AELSSFLRLLTFCMLFSKKPFPIFLESAGYSLEDVLIQKPKAGILKPAFTIIRDSNSKCF 180
           AELSSFLRLLTFCMLFSKKPFPIFLESAGYSLEDVLIQKPKAGILKPAFTIIRDSNSKCF
Sbjct: 121 AELSSFLRLLTFCMLFSKKPFPIFLESAGYSLEDVLIQKPKAGILKPAFTIIRDSNSKCF 180

Query: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240
           LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF
Sbjct: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240

Query: 241 LLKALDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300
           LLKALDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG
Sbjct: 241 LLKALDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300

Query: 301 KQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360
           KQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASALGS
Sbjct: 301 KQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360

Query: 361 RLPSMATAKAKVAGAGALLRPVSSSTQDVVKRAVVRTRSSLSSWSCIGARRRISNPT-EL 420
           RLPSMATAKAKVAGAGALLRPVSSSTQDVVKRAVVRTRSSLSSWSCIGARRRISNPT EL
Sbjct: 361 RLPSMATAKAKVAGAGALLRPVSSSTQDVVKRAVVRTRSSLSSWSCIGARRRISNPTEEL 420

Query: 421 PEAPLIAEEVTINATEKKKLRSGSSSDDSSDHDTDEERHLIHEERIVTSTDVEDITDGEL 480
           PEAPLIAEEVTINATEKKKLRSGSSSDDSSDHDTDEERHLIHEERIVTSTDVEDITDGEL
Sbjct: 421 PEAPLIAEEVTINATEKKKLRSGSSSDDSSDHDTDEERHLIHEERIVTSTDVEDITDGEL 480

Query: 481 WYELEKELQRQEKKVDVITREVGAAAAKEIEEEEEEEEERLLTDAEGSSEKPLSSLDASE 540
           WYELEKELQRQEKKVDVITREVGAAAAKEI  EEEEEEERLLTDAEGSSEKPLSSLDASE
Sbjct: 481 WYELEKELQRQEKKVDVITREVGAAAAKEI--EEEEEEERLLTDAEGSSEKPLSSLDASE 540

Query: 541 NIRFYPPGRTMHIVSVLSPDSDNLAHDEEIVQEKVGIYETPRELYSKLRLSRTMINDHYM 600
           NIRFYPPGRTMHIVSVLSPDSDNLAH EEIVQEKVGIYETPRELYSKLRLSRTMINDHYM
Sbjct: 541 NIRFYPPGRTMHIVSVLSPDSDNLAHAEEIVQEKVGIYETPRELYSKLRLSRTMINDHYM 600

Query: 601 PMYKKMMELLIKELEEDVSSSYEL 624
           PMYKKMMELLIKELEEDVSSSYEL
Sbjct: 601 PMYKKMMELLIKELEEDVSSSYEL 622

BLAST of MS019313 vs. ExPASy TrEMBL
Match: A0A5D3BT55 (Sn1-specific diacylglycerol lipase alpha OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00010 PE=4 SV=1)

HSP 1 Score: 1031.9 bits (2667), Expect = 1.1e-297
Identity = 560/643 (87.09%), Postives = 586/643 (91.14%), Query Frame = 0

Query: 1   MAAGAMATYAGAALIIYYLLSRRLASKGNDDDRSGNMSKSIRSVRRRISRRPAQAPATWF 60
           MAAGAMATYAGAALIIYYLLSRRLA+KG++DDRSGN+SKSIRS RRRISRRPAQAPATWF
Sbjct: 1   MAAGAMATYAGAALIIYYLLSRRLAAKGDEDDRSGNLSKSIRSGRRRISRRPAQAPATWF 60

Query: 61  ETITTLSETLRFTYAETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII 120
           ETITTLSETLRFTY+ETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII
Sbjct: 61  ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII 120

Query: 121 AELSSFLRLLTFCMLFSKKPFPIFLESAGYSLEDVLIQKPKAGILKPAFTIIRDSNSKCF 180
           AEL SFLRLLTFCMLFSKKPFPIFLESAGYS E+VLIQKPKAGILKPAFTIIRDS+SKCF
Sbjct: 121 AELKSFLRLLTFCMLFSKKPFPIFLESAGYSQEEVLIQKPKAGILKPAFTIIRDSSSKCF 180

Query: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240
           LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF
Sbjct: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240

Query: 241 LLKALDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300
           LLK LDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG
Sbjct: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300

Query: 301 KQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360
           KQFITTIINGSDLVPSFSAASIDDLR EVTASSWLNDLRDQVERTRVLNVVYRSASALGS
Sbjct: 301 KQFITTIINGSDLVPSFSAASIDDLRSEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360

Query: 361 RLPSMATAKAKVAGAGALLRPVSSSTQDVVKRAVVRTRSSLSSWSCIGARRR----ISNP 420
           RLPS+ATAKAKVAGAGALLRPVS++TQ  VK AVVRTRSSLSSWSC+GARRR    +SNP
Sbjct: 361 RLPSIATAKAKVAGAGALLRPVSTTTQAAVKSAVVRTRSSLSSWSCMGARRRNGGILSNP 420

Query: 421 T-ELPEAP---------LIAEEVTINATE-KKKLRSGSSS-DDSSDHDTDEER-HLIHEE 480
           T ELPE P         LI+EEVTIN  E KKK  SGSSS DD+SDHDTDEER HLI EE
Sbjct: 421 TEELPEVPRITERNHESLISEEVTINGIEKKKKTESGSSSCDDNSDHDTDEERHHLITEE 480

Query: 481 RIVTSTDVEDITDGELWYELEKELQRQEKKVDVITRE-VGAAAAKEIEEEEEEEEERLLT 540
           RI+ STDVEDITDGELWYELEKELQRQE+KVD  TRE   A  AKEI+EEEE     +LT
Sbjct: 481 RIIASTDVEDITDGELWYELEKELQRQERKVDANTREGKVATVAKEIKEEEES----MLT 540

Query: 541 DAEGSSEKPLSSLDASENIRFYPPGRTMHIVSVLSPDSDNLAHD--EEIVQEKVGIYETP 600
           D EGSSEKPLSSLDASEN+RFYPPG+TMHIVS  SP+SDNL  D  +E +QE VGIYETP
Sbjct: 541 DVEGSSEKPLSSLDASENVRFYPPGKTMHIVSTPSPNSDNLVQDDEDESMQEIVGIYETP 600

Query: 601 RELYSKLRLSRTMINDHYMPMYKKMMELLIKELEEDVSSSYEL 624
           RELYSKLRLSRTMINDHYMPMYKKMME LI +LE DV S+YE+
Sbjct: 601 RELYSKLRLSRTMINDHYMPMYKKMMESLINQLENDVISNYEM 639

BLAST of MS019313 vs. ExPASy TrEMBL
Match: A0A1S3C7B1 (uncharacterized protein LOC103497826 OS=Cucumis melo OX=3656 GN=LOC103497826 PE=4 SV=1)

HSP 1 Score: 1031.9 bits (2667), Expect = 1.1e-297
Identity = 560/643 (87.09%), Postives = 586/643 (91.14%), Query Frame = 0

Query: 1   MAAGAMATYAGAALIIYYLLSRRLASKGNDDDRSGNMSKSIRSVRRRISRRPAQAPATWF 60
           MAAGAMATYAGAALIIYYLLSRRLA+KG++DDRSGN+SKSIRS RRRISRRPAQAPATWF
Sbjct: 1   MAAGAMATYAGAALIIYYLLSRRLAAKGDEDDRSGNLSKSIRSGRRRISRRPAQAPATWF 60

Query: 61  ETITTLSETLRFTYAETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII 120
           ETITTLSETLRFTY+ETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII
Sbjct: 61  ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII 120

Query: 121 AELSSFLRLLTFCMLFSKKPFPIFLESAGYSLEDVLIQKPKAGILKPAFTIIRDSNSKCF 180
           AEL SFLRLLTFCMLFSKKPFPIFLESAGYS E+VLIQKPKAGILKPAFTIIRDS+SKCF
Sbjct: 121 AELKSFLRLLTFCMLFSKKPFPIFLESAGYSQEEVLIQKPKAGILKPAFTIIRDSSSKCF 180

Query: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240
           LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF
Sbjct: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240

Query: 241 LLKALDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300
           LLK LDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG
Sbjct: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300

Query: 301 KQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360
           KQFITTIINGSDLVPSFSAASIDDLR EVTASSWLNDLRDQVERTRVLNVVYRSASALGS
Sbjct: 301 KQFITTIINGSDLVPSFSAASIDDLRSEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360

Query: 361 RLPSMATAKAKVAGAGALLRPVSSSTQDVVKRAVVRTRSSLSSWSCIGARRR----ISNP 420
           RLPS+ATAKAKVAGAGALLRPVS++TQ  VK AVVRTRSSLSSWSC+GARRR    +SNP
Sbjct: 361 RLPSIATAKAKVAGAGALLRPVSTTTQAAVKSAVVRTRSSLSSWSCMGARRRNGGILSNP 420

Query: 421 T-ELPEAP---------LIAEEVTINATE-KKKLRSGSSS-DDSSDHDTDEER-HLIHEE 480
           T ELPE P         LI+EEVTIN  E KKK  SGSSS DD+SDHDTDEER HLI EE
Sbjct: 421 TEELPEVPRITERNHESLISEEVTINGIEKKKKTESGSSSCDDNSDHDTDEERHHLITEE 480

Query: 481 RIVTSTDVEDITDGELWYELEKELQRQEKKVDVITRE-VGAAAAKEIEEEEEEEEERLLT 540
           RI+ STDVEDITDGELWYELEKELQRQE+KVD  TRE   A  AKEI+EEEE     +LT
Sbjct: 481 RIIASTDVEDITDGELWYELEKELQRQERKVDANTREGKVATVAKEIKEEEES----MLT 540

Query: 541 DAEGSSEKPLSSLDASENIRFYPPGRTMHIVSVLSPDSDNLAHD--EEIVQEKVGIYETP 600
           D EGSSEKPLSSLDASEN+RFYPPG+TMHIVS  SP+SDNL  D  +E +QE VGIYETP
Sbjct: 541 DVEGSSEKPLSSLDASENVRFYPPGKTMHIVSTPSPNSDNLVQDDEDESMQEIVGIYETP 600

Query: 601 RELYSKLRLSRTMINDHYMPMYKKMMELLIKELEEDVSSSYEL 624
           RELYSKLRLSRTMINDHYMPMYKKMME LI +LE DV S+YE+
Sbjct: 601 RELYSKLRLSRTMINDHYMPMYKKMMESLINQLENDVISNYEM 639

BLAST of MS019313 vs. ExPASy TrEMBL
Match: A0A0A0KI10 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G314110 PE=4 SV=1)

HSP 1 Score: 1021.9 bits (2641), Expect = 1.1e-294
Identity = 551/641 (85.96%), Postives = 578/641 (90.17%), Query Frame = 0

Query: 1   MAAGAMATYAGAALIIYYLLSRRLASKGNDDDRSGNMSKSIRSVRRRISRRPAQAPATWF 60
           MAAGAMATYAGAALIIYY LSRRLA+KG++DDRSGN+SKSIRS RRRISRRPAQAPATWF
Sbjct: 1   MAAGAMATYAGAALIIYYFLSRRLAAKGDEDDRSGNLSKSIRSCRRRISRRPAQAPATWF 60

Query: 61  ETITTLSETLRFTYAETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII 120
           ETITTLSETLRFTY+ETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII
Sbjct: 61  ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII 120

Query: 121 AELSSFLRLLTFCMLFSKKPFPIFLESAGYSLEDVLIQKPKAGILKPAFTIIRDSNSKCF 180
           AEL SFLRLLTFCMLFSKKPFPIFLESAGYS E+VLIQKPKAGILKPAFTIIRDS+SKCF
Sbjct: 121 AELKSFLRLLTFCMLFSKKPFPIFLESAGYSQEEVLIQKPKAGILKPAFTIIRDSSSKCF 180

Query: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240
           LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF
Sbjct: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240

Query: 241 LLKALDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300
           LLK LDDFPDYKIKIVGHSLGGGTAALLTYILREQ EFSSSTCITFAPAACMTWELAESG
Sbjct: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQTEFSSSTCITFAPAACMTWELAESG 300

Query: 301 KQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360
           KQFITTIINGSDLVPSFSAASIDDLR EVTASSWLNDLRDQVERTRVLNVVYRSASALGS
Sbjct: 301 KQFITTIINGSDLVPSFSAASIDDLRSEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360

Query: 361 RLPSMATAKAKVAGAGALLRPVSSSTQDVVKRAVVRTRSSLSSWSCIGARRR----ISNP 420
           RLPS+ATAKAKVAGAGALLRPVS++TQ  VK AVVRTRSSLSSWSC+GARRR    +SNP
Sbjct: 361 RLPSIATAKAKVAGAGALLRPVSTTTQAAVKSAVVRTRSSLSSWSCMGARRRNGNILSNP 420

Query: 421 T-ELPEAPLI---------AEEVTINATEKKKL-RSGSSSDDSSDHDTDEER-HLIHEER 480
           T ELPE PL+          EEV IN  EKKK    GSS DDSSDHDTDEE+ H+I  ER
Sbjct: 421 TEELPEVPLMTERNHESLKCEEVRINGIEKKKKPEFGSSCDDSSDHDTDEEQHHIITGER 480

Query: 481 IVTSTDVEDITDGELWYELEKELQRQEKKVDVITREVGAAAAKEIEEEEEEEEERLLTDA 540
           I+ STDVEDITDGELWYELEKELQRQEKKVD  TRE   A    + +E +EEEE +LTD 
Sbjct: 481 IIASTDVEDITDGELWYELEKELQRQEKKVDANTREAKVAT---VGKEIKEEEESMLTDV 540

Query: 541 EGSSEKPLSSLDASENIRFYPPGRTMHIVSVLSPDSDNLAHD--EEIVQEKVGIYETPRE 600
           EGSSEKPLSSLDASENIRFYPPG+TMHIVS  SP+SDNL  D  +E  QE VGIYETPRE
Sbjct: 541 EGSSEKPLSSLDASENIRFYPPGKTMHIVSTPSPNSDNLVQDDEDESTQEIVGIYETPRE 600

Query: 601 LYSKLRLSRTMINDHYMPMYKKMMELLIKELEEDVSSSYEL 624
           LYSKLRLSRTMINDHYMPMYKKMME LI +LE+DV S+YE+
Sbjct: 601 LYSKLRLSRTMINDHYMPMYKKMMESLINQLEKDVISNYEM 638

BLAST of MS019313 vs. ExPASy TrEMBL
Match: A0A6J1H5B8 (uncharacterized protein LOC111460272 OS=Cucurbita moschata OX=3662 GN=LOC111460272 PE=4 SV=1)

HSP 1 Score: 1002.3 bits (2590), Expect = 9.0e-289
Identity = 550/652 (84.36%), Postives = 584/652 (89.57%), Query Frame = 0

Query: 1   MAAGAMATYAGAALIIYYLLSRRLASKGNDDDRSGNMSKSIRSVRRRISRRPAQAPATWF 60
           MAAGA+ATYAGAAL++YYLLSRRLA+KG++DDRS N+S+SIRS RRRISRRPAQAPATWF
Sbjct: 1   MAAGALATYAGAALLMYYLLSRRLAAKGDEDDRSDNLSESIRSGRRRISRRPAQAPATWF 60

Query: 61  ETITTLSETLRFTYAETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII 120
           ETITTLSETLRFTYAETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQL+GPEII
Sbjct: 61  ETITTLSETLRFTYAETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLRGPEII 120

Query: 121 AELSSFLRLLTFCMLFSKKPFPIFLESAGYSLEDVLIQKPKAGILKPAFTIIRDSNSKCF 180
           AEL SFLRLLTFCMLFSKKPFPIFLESAGYS EDVLIQKPKAGILKPAFTIIRDSNSKCF
Sbjct: 121 AELKSFLRLLTFCMLFSKKPFPIFLESAGYSQEDVLIQKPKAGILKPAFTIIRDSNSKCF 180

Query: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240
           LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTP 
Sbjct: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPR 240

Query: 241 LLKALDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300
           LLK LDDFPDYKIKIVGHSLGGGTAALLTYILRE+K+FSS+TCI FAPAACMTWELAESG
Sbjct: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILRERKDFSSTTCIAFAPAACMTWELAESG 300

Query: 301 KQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360
           KQFITTIIN SDLVPSFSAASIDDLR EVTASSWLNDLRDQVERTRVL+VVYRSASALGS
Sbjct: 301 KQFITTIINSSDLVPSFSAASIDDLRSEVTASSWLNDLRDQVERTRVLSVVYRSASALGS 360

Query: 361 RLPSMATAKAKVAGAGALLRPVSSSTQDVVKRAVVRTRSSLSSWSCIGARRR----ISNP 420
           RLPS+ATAKAKVAGAGALLRPVS++TQ  VK AVVRTRSSL+SWSC+GARRR    + NP
Sbjct: 361 RLPSIATAKAKVAGAGALLRPVSTTTQTAVKSAVVRTRSSLASWSCMGARRRNGGLLPNP 420

Query: 421 T-ELPEAPLIAE----------EVTINAT--EKKKLRSGSSS---DDSSDHDTDEER-HL 480
           T ELPEAPL  E          EVT N T  +KKKL SGSSS   DDSSDHDTD+ER HL
Sbjct: 421 TEELPEAPLRTERKHESLIADDEVTTNNTILKKKKLESGSSSLGDDDSSDHDTDDERHHL 480

Query: 481 IHEER-IVTSTDVEDITDGELWYELEKELQRQEKK----VDVITREVG-AAAAKEIEEEE 540
           I+E++ + +STDV+DITDGELWYELEKELQRQEKK     DVITRE   AA AKEI EEE
Sbjct: 481 INEDKTVASSTDVDDITDGELWYELEKELQRQEKKTKTTTDVITREANVAAVAKEIVEEE 540

Query: 541 EEEEERLLTDAEGSSEKPLSSLDASENIRFYPPGRTMHIVSVLSPDSDN-LAHDEEIVQE 600
           E     +LT+ EGSSEKPLSSLDASENIRFYPPG+TMHIVSV S DSDN   HD EIVQE
Sbjct: 541 ES----MLTEVEGSSEKPLSSLDASENIRFYPPGKTMHIVSVPSSDSDNSFVHDNEIVQE 600

Query: 601 KVGIYETPRELYSKLRLSRTMINDHYMPMYKKMMELLIKELEEDVSSS-YEL 624
           +VGIYETPR LY KLRLSRTMINDHYMPMYKKM+ELLI +LE DVSSS YE+
Sbjct: 601 RVGIYETPRGLYGKLRLSRTMINDHYMPMYKKMIELLINQLERDVSSSNYEM 648

BLAST of MS019313 vs. TAIR 10
Match: AT4G16070.1 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 780.0 bits (2013), Expect = 1.4e-225
Identity = 434/660 (65.76%), Postives = 508/660 (76.97%), Query Frame = 0

Query: 1   MAAGAMATYAGAALIIYYLLSRRLASKGNDDDRSGNMSKSIRSVRRRISRRPAQAPATWF 60
           MAAG M T  GA +I+Y L  R + ++  +DD  G + KS RS RRRI RRPAQAPATW 
Sbjct: 1   MAAGVMVTATGAVVILYLLSRRIVWARNGEDDSGGELGKSGRSGRRRIVRRPAQAPATWL 60

Query: 61  ETITTLSETLRFTYAETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII 120
           ETI+TLSETLRFTY+ETLGKWPI DLAFGINYLMRRQGN   A+VYAG++ ++LKGPEII
Sbjct: 61  ETISTLSETLRFTYSETLGKWPIADLAFGINYLMRRQGNFPTASVYAGSNCIELKGPEII 120

Query: 121 AELSSFLRLLTFCMLFSKKPFPIFLESAGYSLEDVLIQKPKAGILKPAFTIIRDSNSKCF 180
            +L+  LR LT CMLFSKKPF +FLESAGY+ EDVL+QKPKAGI++PAFTIIRD+NSKC 
Sbjct: 121 MDLTELLRFLTLCMLFSKKPFAVFLESAGYTHEDVLLQKPKAGIMQPAFTIIRDTNSKCI 180

Query: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240
           LLLIRGTHSIKDTLTA TGAVVPFHHSVLHDGG+SNLVLGYAH GMVAAARWIAKLS P 
Sbjct: 181 LLLIRGTHSIKDTLTAATGAVVPFHHSVLHDGGLSNLVLGYAHCGMVAAARWIAKLSVPC 240

Query: 241 LLKALDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300
           LLKALD+ P +K++IVGHSLGGGTA+LLTYILREQKEF+S+TC TFAPAACMTW+LAESG
Sbjct: 241 LLKALDENPSFKVQIVGHSLGGGTASLLTYILREQKEFASATCFTFAPAACMTWDLAESG 300

Query: 301 KQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360
           K FITTIINGSDLVP+FSA+S+DDLR EVT+SSW NDLRDQVE TRVL+VVYRSA+A+GS
Sbjct: 301 KHFITTIINGSDLVPTFSASSVDDLRSEVTSSSWSNDLRDQVEHTRVLSVVYRSATAIGS 360

Query: 361 RLPSMATAKAKVAGAGALLRPVSSSTQDVVKR------AVVRTRSSLSSWSCIGARRRI- 420
           RLPS+A+AKAKVAGAGA+LRPVSS TQ ++KR      AVV+TRS+LSSWSCIG RRR  
Sbjct: 361 RLPSIASAKAKVAGAGAILRPVSSGTQVMLKRAQDVAQAVVQTRSTLSSWSCIGPRRRAI 420

Query: 421 -----SNPTELPEA-----------PLIAEEVTINATEKKKLRSGSSSDDSSDHD----T 480
                S  T++PEA            L+AE V I+    K+    SSS   SD D     
Sbjct: 421 SSQLNSKVTDMPEASAIMAERRSTEALLAETVAIDRKGHKRTEHSSSSSSESDRDEPDEE 480

Query: 481 DEERHLIHEERIV--TSTDVEDITDGELWYELEKELQRQEKKVDVITREVGAAAAKEIEE 540
           +EE  LI  ++++  TS+  ED+T+GELW EL++EL RQE + D    E  AAAAKEI  
Sbjct: 481 EEEEPLISIDQVIAETSSIEEDVTEGELWDELDRELTRQENERDSEAMEEEAAAAKEI-- 540

Query: 541 EEEEEEERLLT----DAEGSSEKPL--SSLDASENIRFYPPGRTMHIVSVLSPDSDNLAH 600
               EEE ++T     + G ++ P+  SS+D  EN RFYPPG+ MHIVSV   +S+    
Sbjct: 541 ---TEEETVITGGGDSSTGQNQSPVSASSMDLIENQRFYPPGKIMHIVSVTETESET-ER 600

Query: 601 DEEIV-----QEKVGIYETPRELYSKLRLSRTMINDHYMPMYKKMMELLIKELEEDVSSS 621
           DE +V      E+V IYETPRELY K+RLSRTMINDHYMPMYKKMMELLI ELE D  SS
Sbjct: 601 DEVVVVGTTTVERVRIYETPRELYRKIRLSRTMINDHYMPMYKKMMELLITELECDSHSS 654

BLAST of MS019313 vs. TAIR 10
Match: AT4G16070.2 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 755.7 bits (1950), Expect = 2.8e-218
Identity = 427/660 (64.70%), Postives = 500/660 (75.76%), Query Frame = 0

Query: 1   MAAGAMATYAGAALIIYYLLSRRLASKGNDDDRSGNMSKSIRSVRRRISRRPAQAPATWF 60
           MAAG M T  GA +I+Y L  R + ++  +DD  G + KS RS RRRI RRPAQAPATW 
Sbjct: 1   MAAGVMVTATGAVVILYLLSRRIVWARNGEDDSGGELGKSGRSGRRRIVRRPAQAPATWL 60

Query: 61  ETITTLSETLRFTYAETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII 120
           ETI+TLSETLRFTY+ETLGKWPI DLAFGINYLMRRQGN   A+VYAG++ ++LKGPEII
Sbjct: 61  ETISTLSETLRFTYSETLGKWPIADLAFGINYLMRRQGNFPTASVYAGSNCIELKGPEII 120

Query: 121 AELSSFLRLLTFCMLFSKKPFPIFLESAGYSLEDVLIQKPKAGILKPAFTIIRDSNSKCF 180
            +L+  LR LT CMLFSKKPF +FLESAGY+ EDVL+QKPKAGI++PAFTIIRD+NSKC 
Sbjct: 121 MDLTELLRFLTLCMLFSKKPFAVFLESAGYTHEDVLLQKPKAGIMQPAFTIIRDTNSKCI 180

Query: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240
           LLLIRGTHSIKDTLTA TGAVVPFHHSVLHDGG+SNLVLGYAH GMVAAARWIAKLS P 
Sbjct: 181 LLLIRGTHSIKDTLTAATGAVVPFHHSVLHDGGLSNLVLGYAHCGMVAAARWIAKLSVPC 240

Query: 241 LLKALDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300
           LLKALD+ P +K++IVGHSLGGGTA+LLTYILREQKEF+S+TC TFAP        AESG
Sbjct: 241 LLKALDENPSFKVQIVGHSLGGGTASLLTYILREQKEFASATCFTFAP--------AESG 300

Query: 301 KQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360
           K FITTIINGSDLVP+FSA+S+DDLR EVT+SSW NDLRDQVE TRVL+VVYRSA+A+GS
Sbjct: 301 KHFITTIINGSDLVPTFSASSVDDLRSEVTSSSWSNDLRDQVEHTRVLSVVYRSATAIGS 360

Query: 361 RLPSMATAKAKVAGAGALLRPVSSSTQDVVKR------AVVRTRSSLSSWSCIGARRRI- 420
           RLPS+A+AKAKVAGAGA+LRPVSS TQ ++KR      AVV+TRS+LSSWSCIG RRR  
Sbjct: 361 RLPSIASAKAKVAGAGAILRPVSSGTQVMLKRAQDVAQAVVQTRSTLSSWSCIGPRRRAI 420

Query: 421 -----SNPTELPEA-----------PLIAEEVTINATEKKKLRSGSSSDDSSDHD----T 480
                S  T++PEA            L+AE V I+    K+    SSS   SD D     
Sbjct: 421 SSQLNSKVTDMPEASAIMAERRSTEALLAETVAIDRKGHKRTEHSSSSSSESDRDEPDEE 480

Query: 481 DEERHLIHEERIV--TSTDVEDITDGELWYELEKELQRQEKKVDVITREVGAAAAKEIEE 540
           +EE  LI  ++++  TS+  ED+T+GELW EL++EL RQE + D    E  AAAAKEI  
Sbjct: 481 EEEEPLISIDQVIAETSSIEEDVTEGELWDELDRELTRQENERDSEAMEEEAAAAKEI-- 540

Query: 541 EEEEEEERLLT----DAEGSSEKPL--SSLDASENIRFYPPGRTMHIVSVLSPDSDNLAH 600
               EEE ++T     + G ++ P+  SS+D  EN RFYPPG+ MHIVSV   +S+    
Sbjct: 541 ---TEEETVITGGGDSSTGQNQSPVSASSMDLIENQRFYPPGKIMHIVSVTETESET-ER 600

Query: 601 DEEIV-----QEKVGIYETPRELYSKLRLSRTMINDHYMPMYKKMMELLIKELEEDVSSS 621
           DE +V      E+V IYETPRELY K+RLSRTMINDHYMPMYKKMMELLI ELE D  SS
Sbjct: 601 DEVVVVGTTTVERVRIYETPRELYRKIRLSRTMINDHYMPMYKKMMELLITELECDSHSS 646

BLAST of MS019313 vs. TAIR 10
Match: AT3G14075.1 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 619.0 bits (1595), Expect = 4.1e-177
Identity = 355/651 (54.53%), Postives = 452/651 (69.43%), Query Frame = 0

Query: 1   MAAGAMATYAGAALIIYYLLSRRLASKGND-DDRSGNMSKSIRSVR-RRISRRPAQAPAT 60
           MA   MAT AGAA ++YY L+R+L +  +D DD +   S S  S+R  R+S R  QAPAT
Sbjct: 1   MATATMATAAGAAALLYYTLNRKLIAGPSDVDDENSEASASRPSLRIDRVSHRLIQAPAT 60

Query: 61  WFETITTLSETLRFTYAETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPE 120
           W ETI+TLSETLRFTY+ETLGKWPIGDLAFGIN+L++RQG L V  V+ G DSV+L+G E
Sbjct: 61  WLETISTLSETLRFTYSETLGKWPIGDLAFGINFLIKRQGLLHVDRVFGGKDSVELRGSE 120

Query: 121 IIAELSSFLRLLTFCMLFSKKPFPIFLESAGYSLEDVLIQKPKAGILKPAFTIIRDSNSK 180
           +  EL   L LLT C  FSKK FP FLE  G++ E+VLI +PKAGILKPAFT++ D N+K
Sbjct: 121 VATELKYLLHLLTLCWHFSKKSFPFFLEETGFTKENVLIHEPKAGILKPAFTVLVDHNTK 180

Query: 181 CFLLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLST 240
            FLLLIRGTHSIKDTLTA TGA+VPFHH+V+++ G+SNLVLGYAH GMVAAAR IAKL+T
Sbjct: 181 YFLLLIRGTHSIKDTLTAATGAIVPFHHTVVNERGVSNLVLGYAHCGMVAAARCIAKLAT 240

Query: 241 PFLLKALDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAE 300
           P LLK L+ +PDYKIKIVGHSLGGGTAALLTYI+REQK  S++TC+TFAPAACMTWELA+
Sbjct: 241 PCLLKGLEQYPDYKIKIVGHSLGGGTAALLTYIMREQKMLSTATCVTFAPAACMTWELAD 300

Query: 301 SGKQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASAL 360
           SG  FI ++ING+DLVP+FSAA++DDLR EVTAS+WLNDLR+Q+E TR+L+ VYRSA+AL
Sbjct: 301 SGNDFIVSVINGADLVPTFSAAAVDDLRAEVTASAWLNDLRNQIEHTRILSTVYRSATAL 360

Query: 361 GSRLPSMATAKAKVAGAGALLRPVSSSTQDVVKRA---VVRTRSSLSSWSCIGARRRIS- 420
           GSRLPSMATAKAKVAGAGA+LRPVSS TQ V++RA   + R   S+SSWSC+G RRR S 
Sbjct: 361 GSRLPSMATAKAKVAGAGAMLRPVSSGTQVVMRRAQSMLTRPALSISSWSCMGPRRRASA 420

Query: 421 --------------NPTELPEA--PLIAEEVTINATEKKKLRSGSSSDDS---SDHDTDE 480
                            ++PE   PL+  +  I    K +    +  + S      D DE
Sbjct: 421 TQSISEHQLDTSEAMSQDIPETSDPLLVTDEEITGKWKSEAECSNYEETSPRLGATDLDE 480

Query: 481 ERHLIHEERIVTSTDVEDITDGELWYELEKELQRQEKKVDVITREVGAAAAKEIEEEEEE 540
                 E+     T  E +T+ ELW +LE +L     +    T         ++ +E +E
Sbjct: 481 -----CEDPAEMDTREERMTEAELWQQLEHDLYHDSSEQPEET---------DVAKEIKE 540

Query: 541 EEERLLTDAEGSSEKPLSSLDASENIRFYPPGRTMHIVSVL--------SPDSDNLAHD- 600
           EEE ++ +A G +     + +  E+ RF P G+ MHIV+V           D D  A + 
Sbjct: 541 EEEAVIAEA-GVAPPESQTAEMKESRRFLPAGKIMHIVTVRPEAVEPNEEEDEDGSALER 600

Query: 601 ----EEIVQEKVGIYETPRELYSKLRLSRTMINDHYMPMYKKMMELLIKEL 614
               E + + +VGI+ TPR LYSK+RLS+ MI+DH+MP+Y++ +E LI+EL
Sbjct: 601 PETVETVEEGRVGIFLTPRSLYSKVRLSQRMISDHFMPVYRRQIERLIQEL 636

BLAST of MS019313 vs. TAIR 10
Match: AT3G14075.2 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 619.0 bits (1595), Expect = 4.1e-177
Identity = 355/651 (54.53%), Postives = 452/651 (69.43%), Query Frame = 0

Query: 1   MAAGAMATYAGAALIIYYLLSRRLASKGND-DDRSGNMSKSIRSVR-RRISRRPAQAPAT 60
           MA   MAT AGAA ++YY L+R+L +  +D DD +   S S  S+R  R+S R  QAPAT
Sbjct: 1   MATATMATAAGAAALLYYTLNRKLIAGPSDVDDENSEASASRPSLRIDRVSHRLIQAPAT 60

Query: 61  WFETITTLSETLRFTYAETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPE 120
           W ETI+TLSETLRFTY+ETLGKWPIGDLAFGIN+L++RQG L V  V+ G DSV+L+G E
Sbjct: 61  WLETISTLSETLRFTYSETLGKWPIGDLAFGINFLIKRQGLLHVDRVFGGKDSVELRGSE 120

Query: 121 IIAELSSFLRLLTFCMLFSKKPFPIFLESAGYSLEDVLIQKPKAGILKPAFTIIRDSNSK 180
           +  EL   L LLT C  FSKK FP FLE  G++ E+VLI +PKAGILKPAFT++ D N+K
Sbjct: 121 VATELKYLLHLLTLCWHFSKKSFPFFLEETGFTKENVLIHEPKAGILKPAFTVLVDHNTK 180

Query: 181 CFLLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLST 240
            FLLLIRGTHSIKDTLTA TGA+VPFHH+V+++ G+SNLVLGYAH GMVAAAR IAKL+T
Sbjct: 181 YFLLLIRGTHSIKDTLTAATGAIVPFHHTVVNERGVSNLVLGYAHCGMVAAARCIAKLAT 240

Query: 241 PFLLKALDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAE 300
           P LLK L+ +PDYKIKIVGHSLGGGTAALLTYI+REQK  S++TC+TFAPAACMTWELA+
Sbjct: 241 PCLLKGLEQYPDYKIKIVGHSLGGGTAALLTYIMREQKMLSTATCVTFAPAACMTWELAD 300

Query: 301 SGKQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASAL 360
           SG  FI ++ING+DLVP+FSAA++DDLR EVTAS+WLNDLR+Q+E TR+L+ VYRSA+AL
Sbjct: 301 SGNDFIVSVINGADLVPTFSAAAVDDLRAEVTASAWLNDLRNQIEHTRILSTVYRSATAL 360

Query: 361 GSRLPSMATAKAKVAGAGALLRPVSSSTQDVVKRA---VVRTRSSLSSWSCIGARRRIS- 420
           GSRLPSMATAKAKVAGAGA+LRPVSS TQ V++RA   + R   S+SSWSC+G RRR S 
Sbjct: 361 GSRLPSMATAKAKVAGAGAMLRPVSSGTQVVMRRAQSMLTRPALSISSWSCMGPRRRASA 420

Query: 421 --------------NPTELPEA--PLIAEEVTINATEKKKLRSGSSSDDS---SDHDTDE 480
                            ++PE   PL+  +  I    K +    +  + S      D DE
Sbjct: 421 TQSISEHQLDTSEAMSQDIPETSDPLLVTDEEITGKWKSEAECSNYEETSPRLGATDLDE 480

Query: 481 ERHLIHEERIVTSTDVEDITDGELWYELEKELQRQEKKVDVITREVGAAAAKEIEEEEEE 540
                 E+     T  E +T+ ELW +LE +L     +    T         ++ +E +E
Sbjct: 481 -----CEDPAEMDTREERMTEAELWQQLEHDLYHDSSEQPEET---------DVAKEIKE 540

Query: 541 EEERLLTDAEGSSEKPLSSLDASENIRFYPPGRTMHIVSVL--------SPDSDNLAHD- 600
           EEE ++ +A G +     + +  E+ RF P G+ MHIV+V           D D  A + 
Sbjct: 541 EEEAVIAEA-GVAPPESQTAEMKESRRFLPAGKIMHIVTVRPEAVEPNEEEDEDGSALER 600

Query: 601 ----EEIVQEKVGIYETPRELYSKLRLSRTMINDHYMPMYKKMMELLIKEL 614
               E + + +VGI+ TPR LYSK+RLS+ MI+DH+MP+Y++ +E LI+EL
Sbjct: 601 PETVETVEEGRVGIFLTPRSLYSKVRLSQRMISDHFMPVYRRQIERLIQEL 636

BLAST of MS019313 vs. TAIR 10
Match: AT2G42450.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 105.9 bits (263), Expect = 1.2e-22
Identity = 84/309 (27.18%), Postives = 144/309 (46.60%), Query Frame = 0

Query: 65  TLSETLRFTYAETLG--KWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEIIAE 124
           +LSE +       LG   W  GDL  G+  +  RQ +L        +    +KG E+++E
Sbjct: 131 SLSEIIASIQRSRLGIEGWTFGDLTIGLYLIYLRQASL--------SPFEDVKGVEVVSE 190

Query: 125 LSSF-----LRLLTFCMLFSKKPFPIFLESAGYSLEDVLIQKPKAGILKPAFTIIRDSNS 184
            + +       L   C   S        ++      ++L     + +++P + I  D   
Sbjct: 191 STVYDLIYNAELAKGCYRDSVSG---LAKNTMLRENNILKFVKDSSVMRPGYYIGVDHRR 250

Query: 185 KCFLLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLS 244
           K  +  IRGTH+I D +T +   V      V  +G  +       HFG   AARW     
Sbjct: 251 KLVVFGIRGTHTIYDLITDI---VSSSDEEVTFEGYST-------HFGTAEAARWFLNHE 310

Query: 245 TPFLLKALDDFPDYKIKIVGHSLGGGTAALLTYIL----REQKEFSSS--TCITFAPAAC 304
              + + L  +  YK+++VGHSLGG  A+L+  +L    RE+  F +   + + +A   C
Sbjct: 311 LQTIRRCLAKYEGYKLRLVGHSLGGAIASLMAIMLKKMPREELGFDAEIISAVGYATPPC 370

Query: 305 MTWELAESGKQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVV 361
           ++ ELAE+  +F+TTI+   D++P  SAAS+  LR E+  + W + + ++ E   VL++V
Sbjct: 371 VSKELAENCSEFVTTIVMQDDIIPRLSAASLARLRNEILQTDWTSVI-EKEEWKNVLDLV 417

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022138525.10.0e+0099.36uncharacterized protein LOC111009670 [Momordica charantia][more]
XP_038874736.13.6e-30087.69uncharacterized protein LOC120067274 [Benincasa hispida][more]
XP_008458404.12.2e-29787.09PREDICTED: uncharacterized protein LOC103497826 [Cucumis melo] >TYK02921.1 Sn1-s... [more]
XP_004150086.12.3e-29485.96uncharacterized protein LOC101210872 [Cucumis sativus] >KGN47411.1 hypothetical ... [more]
KAG6575088.11.7e-28984.66Sn1-specific diacylglycerol lipase alpha, partial [Cucurbita argyrosperma subsp.... [more]
Match NameE-valueIdentityDescription
P0C1S92.8e-1327.60Diacylglycerol lipase-beta OS=Rattus norvegicus OX=10116 GN=Daglb PE=1 SV=1[more]
Q9Y4D28.3e-1329.26Diacylglycerol lipase-alpha OS=Homo sapiens OX=9606 GN=DAGLA PE=1 SV=3[more]
Q6WQJ11.1e-1230.43Diacylglycerol lipase-alpha OS=Mus musculus OX=10090 GN=Dagla PE=1 SV=2[more]
Q5YLM11.1e-1230.43Diacylglycerol lipase-alpha OS=Rattus norvegicus OX=10116 GN=Dagla PE=1 SV=1[more]
Q8NCG72.4e-1227.84Diacylglycerol lipase-beta OS=Homo sapiens OX=9606 GN=DAGLB PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1C9P10.0e+0099.36uncharacterized protein LOC111009670 OS=Momordica charantia OX=3673 GN=LOC111009... [more]
A0A5D3BT551.1e-29787.09Sn1-specific diacylglycerol lipase alpha OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A1S3C7B11.1e-29787.09uncharacterized protein LOC103497826 OS=Cucumis melo OX=3656 GN=LOC103497826 PE=... [more]
A0A0A0KI101.1e-29485.96Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G314110 PE=4 SV=1[more]
A0A6J1H5B89.0e-28984.36uncharacterized protein LOC111460272 OS=Cucurbita moschata OX=3662 GN=LOC1114602... [more]
Match NameE-valueIdentityDescription
AT4G16070.11.4e-22565.76Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 [more]
AT4G16070.22.8e-21864.70Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 [more]
AT3G14075.14.1e-17754.53Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 [more]
AT3G14075.24.1e-17754.53Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 [more]
AT2G42450.11.2e-2227.18alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 507..527
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 506..539
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 507..522
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 435..461
NoneNo IPR availablePANTHERPTHR46023LIPASE CLASS 3 PROTEIN-LIKEcoord: 1..616
NoneNo IPR availablePANTHERPTHR46023:SF6LIPASE CLASS 3 FAMILY PROTEINcoord: 1..616
NoneNo IPR availableCDDcd00519Lipase_3coord: 173..324
e-value: 1.75908E-32
score: 122.97
IPR005592Mono-/di-acylglycerol lipase, N-terminalPFAMPF03893Lipase3_Ncoord: 47..127
e-value: 9.5E-20
score: 70.4
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 153..352
e-value: 2.8E-28
score: 100.8
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 155..319
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 182..319
e-value: 6.1E-23
score: 81.3

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS019313.1MS019313.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016042 lipid catabolic process
biological_process GO:0006629 lipid metabolic process