MS019277 (gene) Bitter gourd (TR) v1

Overview
NameMS019277
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionprotein SRC2-like
Locationscaffold611: 26210 .. 27037 (-)
RNA-Seq ExpressionMS019277
SyntenyMS019277
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTCCACGTGGAGTCTAGAAATCAACGTCGTTTCAGCCAAAGACCTTCCCGAAATTAACCTCGTTTCTAAGATGGCCGCGTACGTCGTCGTTTCACTCACCGGCGGCGGCAACTCCGACCAGAAATTCAAAACCAATGCGGATTCCTCCGGCGGAATAAACCCCAATTGGAACTTCCCGATGAAGTTCAGTGTCAATGAGCTGGCATCCACTCTCGTGTTCACTATTAGGTGCTTCAGAACTCTTGGCGACAGAGACGTCGGTGACGTCCACGTGCCGGTTGCCGACCTTCTCCAGTCCACCGGCAACCGGAACTCCATGCAGCACGTGACCTACCCCGTTCGAAAGCCCTCGGGGAAAACCCACGGCCTTTTGAATTTTCAATATAGAGTACTTGGCGCAACGGCGTCGTTTCGCGATGAAAAGCAAAAGCCCCCCGCCGCTTACGCCACCCGAGGAATTGGACCGCCACCGGAAGGTTATCCTCCGCCTCCGAACCCGGCCGCCGGATACCCCCCTCCGCCGCCGCCGCCGTACAACACTGCCTACGCTCCACCGCCACCCCAGCCTTACGGTTATCCACATTATGGTTACCCGGCGCCGCCGCCGCCGCCGCCGCATTATGGATATCATCCGCCGCCGGGGTATCTACCGGCGGCGGGAGTGGTCGCAAAGAAGGAAACGAAATCGGGAATGGGTAAGATTTTAGCAGTGGGAGCGGGAATTCTCGGTGGATTGGTGTGCGGGGCCATGCTCGGAGAATGTGCGGCGGATCTTGGACAGCCGCCTTCCCGCGGAAGTTCCTCCGGCGACGGCGGCGTGCAC

mRNA sequence

ATGGAGTCCACGTGGAGTCTAGAAATCAACGTCGTTTCAGCCAAAGACCTTCCCGAAATTAACCTCGTTTCTAAGATGGCCGCGTACGTCGTCGTTTCACTCACCGGCGGCGGCAACTCCGACCAGAAATTCAAAACCAATGCGGATTCCTCCGGCGGAATAAACCCCAATTGGAACTTCCCGATGAAGTTCAGTGTCAATGAGCTGGCATCCACTCTCGTGTTCACTATTAGGTGCTTCAGAACTCTTGGCGACAGAGACGTCGGTGACGTCCACGTGCCGGTTGCCGACCTTCTCCAGTCCACCGGCAACCGGAACTCCATGCAGCACGTGACCTACCCCGTTCGAAAGCCCTCGGGGAAAACCCACGGCCTTTTGAATTTTCAATATAGAGTACTTGGCGCAACGGCGTCGTTTCGCGATGAAAAGCAAAAGCCCCCCGCCGCTTACGCCACCCGAGGAATTGGACCGCCACCGGAAGGTTATCCTCCGCCTCCGAACCCGGCCGCCGGATACCCCCCTCCGCCGCCGCCGCCGTACAACACTGCCTACGCTCCACCGCCACCCCAGCCTTACGGTTATCCACATTATGGTTACCCGGCGCCGCCGCCGCCGCCGCCGCATTATGGATATCATCCGCCGCCGGGGTATCTACCGGCGGCGGGAGTGGTCGCAAAGAAGGAAACGAAATCGGGAATGGGTAAGATTTTAGCAGTGGGAGCGGGAATTCTCGGTGGATTGGTGTGCGGGGCCATGCTCGGAGAATGTGCGGCGGATCTTGGACAGCCGCCTTCCCGCGGAAGTTCCTCCGGCGACGGCGGCGTGCAC

Coding sequence (CDS)

ATGGAGTCCACGTGGAGTCTAGAAATCAACGTCGTTTCAGCCAAAGACCTTCCCGAAATTAACCTCGTTTCTAAGATGGCCGCGTACGTCGTCGTTTCACTCACCGGCGGCGGCAACTCCGACCAGAAATTCAAAACCAATGCGGATTCCTCCGGCGGAATAAACCCCAATTGGAACTTCCCGATGAAGTTCAGTGTCAATGAGCTGGCATCCACTCTCGTGTTCACTATTAGGTGCTTCAGAACTCTTGGCGACAGAGACGTCGGTGACGTCCACGTGCCGGTTGCCGACCTTCTCCAGTCCACCGGCAACCGGAACTCCATGCAGCACGTGACCTACCCCGTTCGAAAGCCCTCGGGGAAAACCCACGGCCTTTTGAATTTTCAATATAGAGTACTTGGCGCAACGGCGTCGTTTCGCGATGAAAAGCAAAAGCCCCCCGCCGCTTACGCCACCCGAGGAATTGGACCGCCACCGGAAGGTTATCCTCCGCCTCCGAACCCGGCCGCCGGATACCCCCCTCCGCCGCCGCCGCCGTACAACACTGCCTACGCTCCACCGCCACCCCAGCCTTACGGTTATCCACATTATGGTTACCCGGCGCCGCCGCCGCCGCCGCCGCATTATGGATATCATCCGCCGCCGGGGTATCTACCGGCGGCGGGAGTGGTCGCAAAGAAGGAAACGAAATCGGGAATGGGTAAGATTTTAGCAGTGGGAGCGGGAATTCTCGGTGGATTGGTGTGCGGGGCCATGCTCGGAGAATGTGCGGCGGATCTTGGACAGCCGCCTTCCCGCGGAAGTTCCTCCGGCGACGGCGGCGTGCAC

Protein sequence

MESTWSLEINVVSAKDLPEINLVSKMAAYVVVSLTGGGNSDQKFKTNADSSGGINPNWNFPMKFSVNELASTLVFTIRCFRTLGDRDVGDVHVPVADLLQSTGNRNSMQHVTYPVRKPSGKTHGLLNFQYRVLGATASFRDEKQKPPAAYATRGIGPPPEGYPPPPNPAAGYPPPPPPPYNTAYAPPPPQPYGYPHYGYPAPPPPPPHYGYHPPPGYLPAAGVVAKKETKSGMGKILAVGAGILGGLVCGAMLGECAADLGQPPSRGSSSGDGGVH
Homology
BLAST of MS019277 vs. NCBI nr
Match: XP_022138764.1 (protein SRC2-like [Momordica charantia])

HSP 1 Score: 444.1 bits (1141), Expect = 8.7e-121
Identity = 238/276 (86.23%), Postives = 240/276 (86.96%), Query Frame = 0

Query: 1   MESTWSLEINVVSAKDLPEINLVSKMAAYVVVSLTGGGNSDQKFKTNADSSGGINPNWNF 60
           MESTWSLEINVVSAKDLPEINLVSKMAAYVVVSL GGGNSDQKFKTNADSSGGINPNWNF
Sbjct: 1   MESTWSLEINVVSAKDLPEINLVSKMAAYVVVSLAGGGNSDQKFKTNADSSGGINPNWNF 60

Query: 61  PMKFSVNELASTLVFTIRCFRTLGDRDVGDVHVPVADLLQSTGNRNSMQHVTYPVRKPSG 120
           PMKFSVNELASTLVFTIRCFRTLGDRD                       VTYPVRKPSG
Sbjct: 61  PMKFSVNELASTLVFTIRCFRTLGDRD-----------------------VTYPVRKPSG 120

Query: 121 KTHGLLNFQYRVLGATASFRDEKQKPPAAYATRGIGPPPEGYPPPPNPAAGYPPPPPPPY 180
           KTHGLLNFQYRVLGATASFRDEKQKPPAAYATRGIGPPPEGYPPPP+PAAGYPPPPPPPY
Sbjct: 121 KTHGLLNFQYRVLGATASFRDEKQKPPAAYATRGIGPPPEGYPPPPHPAAGYPPPPPPPY 180

Query: 181 NTAYAPPPPQPYGYPHYGYPAPPPPPPHYGYHPPPGYLPAAGVVAKKETKSGMGKILAVG 240
           NT YAPPPPQPYGYPHYGYPAP          PPPGYLPAAGVVAKKETKSGMGKILAVG
Sbjct: 181 NTGYAPPPPQPYGYPHYGYPAP----------PPPGYLPAAGVVAKKETKSGMGKILAVG 240

Query: 241 AGILGGLVCGAMLGECAADLGQPPSRGSSSGDGGVH 277
           AGILGGL CGAMLGECAADLGQPPSRGSSSG+GGVH
Sbjct: 241 AGILGGLACGAMLGECAADLGQPPSRGSSSGNGGVH 243

BLAST of MS019277 vs. NCBI nr
Match: KAB1214014.1 (hypothetical protein CJ030_MR5G017277 [Morella rubra])

HSP 1 Score: 191.8 bits (486), Expect = 7.8e-45
Identity = 134/271 (49.45%), Postives = 165/271 (60.89%), Query Frame = 0

Query: 6   SLEINVVSAKDLPEINLVSKMAAYVVVSLTGGGNSDQKFKTNADSSGGINPNWNFPMKFS 65
           +LE+ V+SAKDL ++NL SKM  YVVVSL+G      + KTN D  GG NP+WNFPMKF+
Sbjct: 49  TLEVTVLSAKDLQDVNLFSKMDVYVVVSLSGNSQHKLQAKTNVDREGGKNPSWNFPMKFT 108

Query: 66  VNELA-----STLVFTIRCFRTLGDRDVGDVHVPVADLLQSTGNRNSMQHVTYPVRKPSG 125
           +NE        TL F +RC R+LGD+D+G+V VPV +LL STG+  SMQ V Y V KPSG
Sbjct: 109 INEFTVRQNRLTLEFKLRCDRSLGDKDIGNVCVPVQELLDSTGDGKSMQFVRYQVTKPSG 168

Query: 126 KTHGLLNFQYRVLGATASFRDEKQK-----PPAAYATRGI----GPPPEG-YP-PPPNPA 185
           K  G L F Y+     +S     QK     P  AY +  +     PP +G YP P P P 
Sbjct: 169 KPKGELYFSYKFSENVSSSEPLWQKTQAYEPVTAYPSAPVVEPSAPPYDGLYPIPDPPPP 228

Query: 186 AGY---PPPPPPPYN--TAYAPPPPQPYGYPHYGYPAPPPPPPHYGYHPPPGYLPAAGVV 245
           AGY   PPPPPPPY     + PPPP P   P YGYP PPP PP YGY PPPGY       
Sbjct: 229 AGYSYAPPPPPPPYQPYAYWYPPPPPP---PGYGYPPPPPTPPGYGYPPPPGYAYPPVQQ 288

Query: 246 AKKETKSGMGKILAVGAGILGGLVCGAMLGE 256
            +K+ K GMG    +GAG++GGL+ G +LG+
Sbjct: 289 PRKQNKFGMG----LGAGVVGGLLGGLLLGD 312

BLAST of MS019277 vs. NCBI nr
Match: KAA8548920.1 (hypothetical protein F0562_000604 [Nyssa sinensis])

HSP 1 Score: 184.1 bits (466), Expect = 1.6e-42
Identity = 130/285 (45.61%), Postives = 165/285 (57.89%), Query Frame = 0

Query: 6   SLEINVVSAKDLPEINLVSKMAAYVVVSLTGGGNSDQKFKTNADSSGGINPNWNFPMKFS 65
           +L+IN++SAKDL  +NL+ KM  YVVVS++G   + Q  KT  D +GG NP WNFPMKF+
Sbjct: 5   TLDINIISAKDLKNVNLIDKMDVYVVVSISGDKRTKQ--KTKVDRNGGTNPTWNFPMKFT 64

Query: 66  VNELA-----STLVFTIRCFRTLGDRDVGDVHVPVADLLQSTGNRNSMQHVTYPVRKPSG 125
           V+E A     STL+FT+RC R+LGD+D+G+VHVP+ +LL + G+   MQ V+Y VRKPSG
Sbjct: 65  VDESAAQQNRSTLMFTLRCERSLGDKDIGEVHVPIKELLGNIGD-GKMQFVSYQVRKPSG 124

Query: 126 KTHGLLNFQY----RVLGATASFRD--------------EKQKPPAAYAT------RGIG 185
           K  G  NF Y    +V GA A   +                  PP  YA        G  
Sbjct: 125 KPKGEFNFSYKFGEKVAGAAAKADEPVTAYPAAPVAGTSSAYPPPPQYAAGPYPPPAGGY 184

Query: 186 PPPEGYPPPPNPAAGYPPPPPPPYNTAYAPPPPQPYGYPHYGYPAPPPPPPHYGYHPPPG 245
           PP EGY PPP    GYPPPP P Y   Y PPPPQ  GY       PPPPP  YGY P PG
Sbjct: 185 PPHEGYAPPPVAGYGYPPPPQPQYG-GYPPPPPQYGGY-------PPPPPQGYGYPPQPG 244

Query: 246 YLPAAGVVAKKETKSGMGKILAVGAGILGGLVCGAMLGECAADLG 262
           Y        +K+ K G+G    +GAG+LGG + G ++G+  +D G
Sbjct: 245 YGYQPVQQPQKKNKFGLG----LGAGLLGGALGGLLIGDMISDAG 274

BLAST of MS019277 vs. NCBI nr
Match: KAF3456203.1 (hypothetical protein FNV43_RR00853 [Rhamnella rubrinervis])

HSP 1 Score: 181.0 bits (458), Expect = 1.4e-41
Identity = 130/271 (47.97%), Postives = 161/271 (59.41%), Query Frame = 0

Query: 6   SLEINVVSAKDLPEINLVSKMAAYVVVSLTGGGNSDQKFKTNADSSGGINPNWNFPMKFS 65
           +L+I VVSAK L ++NL+ KM  Y VVS++G  +  QK KTN D   G NP WN+PMKF+
Sbjct: 5   TLDIKVVSAKGLKDVNLIGKMDVYAVVSISGDSHPKQKTKTNVDKDCGSNPTWNYPMKFT 64

Query: 66  VNELAS-----TLVFTIRCFRTLGDRDVGDVHVPVADLLQSTGNRNSMQHVTYPVRKPSG 125
           VNE+A+     TLVF +RC RT GD+D+G V VPV++LL S G+ NSM+ VTY VR  SG
Sbjct: 65  VNEIAAKQNRLTLVFDLRCERTFGDKDIGQVVVPVSELLDSKGDENSMKCVTYQVRTSSG 124

Query: 126 KTHGLLNFQYR-----VLGATASFRDEKQKPPAAYATRGIGPPPEGYPPPPNPAAGYPPP 185
           K  G LNF Y+     V+ A A    +  +P  AY   G GP    YPPP     G  PP
Sbjct: 125 KPKGELNFSYKFGDKVVVEAVA---PKAAEPVTAYPAPGAGPSVPAYPPPAGHPYGAYPP 184

Query: 186 PPPPYNTAYAPPPPQPYG---YPHYGYPAPPPPPPHYGYHPPPGY--LPAAGVVAKKETK 245
           P P Y   Y PPP  PYG    P  GY A PPP P YGY P PGY   P      KK +K
Sbjct: 185 PQPGYG-GYPPPPAAPYGGYQPPPPGY-AYPPPQPGYGYPPAPGYGQPPLQQPQKKKNSK 244

Query: 246 SGMGKILAVGAGILGGLVCGAMLGECAADLG 262
            GMG    +GAG+LGG + G ++G+  +D G
Sbjct: 245 LGMG----LGAGLLGGALGGLLIGDMVSDAG 266

BLAST of MS019277 vs. NCBI nr
Match: XP_041000897.1 (protein SRC2-like [Juglans microcarpa x Juglans regia])

HSP 1 Score: 180.6 bits (457), Expect = 1.8e-41
Identity = 134/280 (47.86%), Postives = 168/280 (60.00%), Query Frame = 0

Query: 6   SLEINVVSAKDLPEINLVSKMAAYVVVSLTGGGNSDQKFKTNADSSGGINPNWNFPMKFS 65
           +LEINV SAKDL ++NL SKM  YVVVSL+G     QK KT  D  GG +P+WNFP+KF+
Sbjct: 5   TLEINVTSAKDLKDLNLFSKMDVYVVVSLSG---DKQKAKTKVDREGGTSPSWNFPVKFT 64

Query: 66  VNELAS-----TLVFTIRCFRTL-GDRDVGDVHVPVADLLQSTGNRNSMQHVTYPVRKPS 125
           ++E A+      L F +RC R+L GD+DVG V+VPVA+LL STG+  SMQ V+Y V KPS
Sbjct: 65  IDESAARQNRLNLEFKLRCDRSLGGDKDVGVVYVPVAELLNSTGDGKSMQFVSYQVTKPS 124

Query: 126 GKTHGLLNFQYRVLGATASFRD------EKQKPPAAYATRGIGP---PPEGYPPPPNPAA 185
           GK  G+LNF Y+ +   A+  +      +  +P  AY    +G    P +G  PP    A
Sbjct: 125 GKPQGMLNFSYKFIEKVAAGGEPLVPEAKAHEPVTAYPAPVVGTSALPYDGLYPP----A 184

Query: 186 GYPPPPPPPYNTAYAPPPPQPYGY----PHYGYPAPPPPPPHYGYHPPP-----GYLPAA 245
             P PPPPPY   Y PPPP  YGY    P YGY   PPPPP YGY PPP     GY P  
Sbjct: 185 AAPHPPPPPYGIGY-PPPPPGYGYPPPPPGYGY---PPPPPGYGYPPPPPPPGYGYQPGV 244

Query: 246 GVVAKKETKSGMGKILAVGAGILGGLVCGAMLGECAADLG 262
               KK  K GMG    +GAG+LGG + G ++G+  +D G
Sbjct: 245 QQPQKK-NKFGMG----LGAGLLGGALGGLLIGDMVSDAG 268

BLAST of MS019277 vs. ExPASy Swiss-Prot
Match: O04133 (Protein SRC2 OS=Glycine max OX=3847 GN=SRC2 PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 4.9e-26
Identity = 108/283 (38.16%), Postives = 141/283 (49.82%), Query Frame = 0

Query: 6   SLEINVVSAKDLPEINLVSKMAAYVVVSLTGGGNSDQKFKTNADSSGGINPNWNFPMKFS 65
           +LE+N++SAKD+  +NL SKM  Y  VSL+G     Q   T+     G NP WN+P+KFS
Sbjct: 7   TLELNIISAKDIKNVNLFSKMDVYAAVSLSGDPLHPQGATTHVHKDAGSNPTWNYPVKFS 66

Query: 66  VNELAS-----TLVFTIRCFRTLGDRDVGDVHVPVADLLQSTGNRNSMQHVTYPVRKPSG 125
           VNE  +     +L   +   RTLGD  +G VHVP+ +LL + G+ +S + V+Y V K S 
Sbjct: 67  VNESLAKENRLSLEIKLISDRTLGDTVIGTVHVPLRELLDNPGDDSSFRQVSYQVMKQSR 126

Query: 126 KTHGLLNFQYRV-------LGATASFRDEKQKPPAAYATRGIGPP--PEGYPPPPNP--- 185
           K+ G LNF Y+           T       Q+P  AY   G G    P G P PP P   
Sbjct: 127 KSKGSLNFSYKFGEHVPAPAAKTPKAAKAGQEPVMAYPPAGAGSSSMPYGTPHPPPPQQY 186

Query: 186 -AAGYPPPPPP-----PYNTAYAPPPPQPYGYPH---YGYPAPPPPPPHYGYHPPPGYLP 245
            A GY  PP       P   AY  PP   YGYP    YGY    P    YGY PP    P
Sbjct: 187 AATGYGYPPQQVHGGYPPQAAYGYPPQTGYGYPQQSGYGY----PQQSGYGY-PPQAQKP 246

Query: 246 AAGVVAKKETKSGMGKILAVGAGILGGLVCGAMLGECAADLGQ 263
                  K+ K GMG    +GAG+LGG + G ++G+  +D  +
Sbjct: 247 -------KKNKFGMG----LGAGLLGGALGGMLIGDMVSDAAE 273

BLAST of MS019277 vs. ExPASy TrEMBL
Match: A0A6J1CB10 (protein SRC2-like OS=Momordica charantia OX=3673 GN=LOC111009847 PE=4 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 4.2e-121
Identity = 238/276 (86.23%), Postives = 240/276 (86.96%), Query Frame = 0

Query: 1   MESTWSLEINVVSAKDLPEINLVSKMAAYVVVSLTGGGNSDQKFKTNADSSGGINPNWNF 60
           MESTWSLEINVVSAKDLPEINLVSKMAAYVVVSL GGGNSDQKFKTNADSSGGINPNWNF
Sbjct: 1   MESTWSLEINVVSAKDLPEINLVSKMAAYVVVSLAGGGNSDQKFKTNADSSGGINPNWNF 60

Query: 61  PMKFSVNELASTLVFTIRCFRTLGDRDVGDVHVPVADLLQSTGNRNSMQHVTYPVRKPSG 120
           PMKFSVNELASTLVFTIRCFRTLGDRD                       VTYPVRKPSG
Sbjct: 61  PMKFSVNELASTLVFTIRCFRTLGDRD-----------------------VTYPVRKPSG 120

Query: 121 KTHGLLNFQYRVLGATASFRDEKQKPPAAYATRGIGPPPEGYPPPPNPAAGYPPPPPPPY 180
           KTHGLLNFQYRVLGATASFRDEKQKPPAAYATRGIGPPPEGYPPPP+PAAGYPPPPPPPY
Sbjct: 121 KTHGLLNFQYRVLGATASFRDEKQKPPAAYATRGIGPPPEGYPPPPHPAAGYPPPPPPPY 180

Query: 181 NTAYAPPPPQPYGYPHYGYPAPPPPPPHYGYHPPPGYLPAAGVVAKKETKSGMGKILAVG 240
           NT YAPPPPQPYGYPHYGYPAP          PPPGYLPAAGVVAKKETKSGMGKILAVG
Sbjct: 181 NTGYAPPPPQPYGYPHYGYPAP----------PPPGYLPAAGVVAKKETKSGMGKILAVG 240

Query: 241 AGILGGLVCGAMLGECAADLGQPPSRGSSSGDGGVH 277
           AGILGGL CGAMLGECAADLGQPPSRGSSSG+GGVH
Sbjct: 241 AGILGGLACGAMLGECAADLGQPPSRGSSSGNGGVH 243

BLAST of MS019277 vs. ExPASy TrEMBL
Match: A0A2N9IAP9 (C2 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS49360 PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 2.9e-45
Identity = 133/281 (47.33%), Postives = 170/281 (60.50%), Query Frame = 0

Query: 6   SLEINVVSAKDLPEINLVSKMAAYVVVSLTGGG-NSDQKFKTNADSSGGINPNWNFPMKF 65
           +LE+N+ SAKDL ++NL+SKM  YVVVSLTG   N  QK KTN    GG NP+WNFPMKF
Sbjct: 5   TLELNIASAKDLKDVNLISKMDVYVVVSLTGDSHNHKQKAKTNVSRDGGTNPSWNFPMKF 64

Query: 66  SVNELAS-----TLVFTIRCFRTLGDRDVGDVHVPVADLLQSTGNRNSMQHVTYPVRKPS 125
           ++ E A+     TL F +RC R+LGD+D+G+V+VP+ +LL S G+  SMQ V Y VR PS
Sbjct: 65  NIEEYAAQQNRLTLQFKLRCDRSLGDKDIGEVYVPIKELLNSRGDTKSMQFVAYQVRTPS 124

Query: 126 GKTHGLLNFQY----RVLGATASFRDEKQKPPAAYATRGIGPPPEGYPPPPNPAAGYPPP 185
           GK+ G LNF Y    +V GATA+ +    +P  AY       P    P  P     YPPP
Sbjct: 125 GKSKGELNFSYKFSEKVSGATAAPKAHVDEPVMAYPATA---PSMVGPSAPYSGGSYPPP 184

Query: 186 PPPPYNTAYAPPPPQPYGYPHYGYPAPPPPPPHYGYHPPP--GYLPAAGVVAKKETKSGM 245
           PP P    Y PPPPQPY  P+ GYP PPPPP  YGY P P  GY P    V + + K+  
Sbjct: 185 PPGP-GYGYPPPPPQPYQQPYGGYP-PPPPPQGYGYPPGPGYGYPPQQPQVQQPQKKNKF 244

Query: 246 GKILAVGAGILGGLVCGAMLGECAADLGQPPSRGSSSGDGG 275
           G  + +GAG+LGG++ G ++G+  +D G          DGG
Sbjct: 245 G--MGMGAGLLGGVLGGLLIGDLVSDGGGFDGGCDGGFDGG 278

BLAST of MS019277 vs. ExPASy TrEMBL
Match: A0A6A1VMH8 (C2 domain-containing protein OS=Morella rubra OX=262757 GN=CJ030_MR5G017277 PE=4 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 3.8e-45
Identity = 134/271 (49.45%), Postives = 165/271 (60.89%), Query Frame = 0

Query: 6   SLEINVVSAKDLPEINLVSKMAAYVVVSLTGGGNSDQKFKTNADSSGGINPNWNFPMKFS 65
           +LE+ V+SAKDL ++NL SKM  YVVVSL+G      + KTN D  GG NP+WNFPMKF+
Sbjct: 49  TLEVTVLSAKDLQDVNLFSKMDVYVVVSLSGNSQHKLQAKTNVDREGGKNPSWNFPMKFT 108

Query: 66  VNELA-----STLVFTIRCFRTLGDRDVGDVHVPVADLLQSTGNRNSMQHVTYPVRKPSG 125
           +NE        TL F +RC R+LGD+D+G+V VPV +LL STG+  SMQ V Y V KPSG
Sbjct: 109 INEFTVRQNRLTLEFKLRCDRSLGDKDIGNVCVPVQELLDSTGDGKSMQFVRYQVTKPSG 168

Query: 126 KTHGLLNFQYRVLGATASFRDEKQK-----PPAAYATRGI----GPPPEG-YP-PPPNPA 185
           K  G L F Y+     +S     QK     P  AY +  +     PP +G YP P P P 
Sbjct: 169 KPKGELYFSYKFSENVSSSEPLWQKTQAYEPVTAYPSAPVVEPSAPPYDGLYPIPDPPPP 228

Query: 186 AGY---PPPPPPPYN--TAYAPPPPQPYGYPHYGYPAPPPPPPHYGYHPPPGYLPAAGVV 245
           AGY   PPPPPPPY     + PPPP P   P YGYP PPP PP YGY PPPGY       
Sbjct: 229 AGYSYAPPPPPPPYQPYAYWYPPPPPP---PGYGYPPPPPTPPGYGYPPPPGYAYPPVQQ 288

Query: 246 AKKETKSGMGKILAVGAGILGGLVCGAMLGE 256
            +K+ K GMG    +GAG++GGL+ G +LG+
Sbjct: 289 PRKQNKFGMG----LGAGVVGGLLGGLLLGD 312

BLAST of MS019277 vs. ExPASy TrEMBL
Match: A0A5J5C465 (Glutathione transferase OS=Nyssa sinensis OX=561372 GN=F0562_000604 PE=4 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 7.8e-43
Identity = 130/285 (45.61%), Postives = 165/285 (57.89%), Query Frame = 0

Query: 6   SLEINVVSAKDLPEINLVSKMAAYVVVSLTGGGNSDQKFKTNADSSGGINPNWNFPMKFS 65
           +L+IN++SAKDL  +NL+ KM  YVVVS++G   + Q  KT  D +GG NP WNFPMKF+
Sbjct: 5   TLDINIISAKDLKNVNLIDKMDVYVVVSISGDKRTKQ--KTKVDRNGGTNPTWNFPMKFT 64

Query: 66  VNELA-----STLVFTIRCFRTLGDRDVGDVHVPVADLLQSTGNRNSMQHVTYPVRKPSG 125
           V+E A     STL+FT+RC R+LGD+D+G+VHVP+ +LL + G+   MQ V+Y VRKPSG
Sbjct: 65  VDESAAQQNRSTLMFTLRCERSLGDKDIGEVHVPIKELLGNIGD-GKMQFVSYQVRKPSG 124

Query: 126 KTHGLLNFQY----RVLGATASFRD--------------EKQKPPAAYAT------RGIG 185
           K  G  NF Y    +V GA A   +                  PP  YA        G  
Sbjct: 125 KPKGEFNFSYKFGEKVAGAAAKADEPVTAYPAAPVAGTSSAYPPPPQYAAGPYPPPAGGY 184

Query: 186 PPPEGYPPPPNPAAGYPPPPPPPYNTAYAPPPPQPYGYPHYGYPAPPPPPPHYGYHPPPG 245
           PP EGY PPP    GYPPPP P Y   Y PPPPQ  GY       PPPPP  YGY P PG
Sbjct: 185 PPHEGYAPPPVAGYGYPPPPQPQYG-GYPPPPPQYGGY-------PPPPPQGYGYPPQPG 244

Query: 246 YLPAAGVVAKKETKSGMGKILAVGAGILGGLVCGAMLGECAADLG 262
           Y        +K+ K G+G    +GAG+LGG + G ++G+  +D G
Sbjct: 245 YGYQPVQQPQKKNKFGLG----LGAGLLGGALGGLLIGDMISDAG 274

BLAST of MS019277 vs. ExPASy TrEMBL
Match: A0A5B7A4U8 (Putative cytokinesis protein sepA-like OS=Davidia involucrata OX=16924 GN=Din_020329 PE=4 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 3.0e-42
Identity = 131/278 (47.12%), Postives = 166/278 (59.71%), Query Frame = 0

Query: 6   SLEINVVSAKDLPEINLVSKMAAYVVVSLTGGGNSDQKFKTNADSSGGINPNWNFPMKFS 65
           +LEINV+SA DL ++N ++KM  YVVVS++G   + QK   N D  G  NP WNFPMKF+
Sbjct: 5   NLEINVISATDLKKVNFITKMDVYVVVSISGEQRTKQKTSVNRD--GDYNPTWNFPMKFT 64

Query: 66  VNELAS-----TLVFTIRCFRTLGDRDVGDVHVPVADLLQSTGNRNSMQHVTYPVRKPSG 125
           V+E A+      L F +RC R+LGD+DVG+V+VP+ DLL + G+   +Q V+Y VRKPSG
Sbjct: 65  VDESAAQQNQLILTFKLRCERSLGDKDVGEVNVPIKDLLATVGD-GKVQFVSYQVRKPSG 124

Query: 126 KTHGLLNFQY----RVLGATASFRDEKQKPPAAY-ATRGIGPPPEGYPPPPNPAAGYPP- 185
           K  G  NF Y    +V GA A+  DE   P  AY AT  +  P   YPPPP P A YPP 
Sbjct: 125 KPKGQFNFSYKFGDKVAGAAAAKVDE---PVTAYPATAPVARPSSAYPPPPPPYAPYPPA 184

Query: 186 -----PPPPPYNTAYAPPPPQPYGYPHYGYPA-------PPPPPPHYGYHPPPGY-LPAA 245
                PPPPP+   Y PPPP P G   YGYP        PPPPPP YGY P PGY  P  
Sbjct: 185 AAGGYPPPPPFG-GYPPPPPPPVG--SYGYPPPTPYGGYPPPPPPGYGYPPQPGYGYPQV 244

Query: 246 GVVAKKETKSGMGKILAVGAGILGGLVCGAMLGECAAD 260
              A +    G+G    +GAG+LGG + G ++G+  +D
Sbjct: 245 QQPAPRRNNLGLG----LGAGLLGGALGGLLIGDMISD 269

BLAST of MS019277 vs. TAIR 10
Match: AT3G16510.1 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 104.4 bits (259), Expect = 1.5e-22
Identity = 116/357 (32.49%), Postives = 152/357 (42.58%), Query Frame = 0

Query: 6   SLEINVVSAKDLPEINLVSKMAAYVVVSLTG-GGNSDQKFKTNADSSGGINPNWNFPMKF 65
           +LE+NV SAKDL  +NL++KM  Y VV +TG     + K KT  D +G   P WN  +KF
Sbjct: 5   TLELNVYSAKDLENVNLITKMDVYAVVWITGDDSRKNHKEKTPIDRTGESEPTWNHTVKF 64

Query: 66  SVNELAS-----TLVFTIRCFRTLGDRDVGDVHVPVADLLQSTGNRNS-------MQHVT 125
           SV++  +     TLV  + C R  GD+D+G+V VPV +LL  + + +S       M+ VT
Sbjct: 65  SVDQRLAHEGRLTLVVKLVCDRIFGDKDLGEVQVPVLELLHGSSSPSSNGDGQGMMRFVT 124

Query: 126 YPVRKPSGKTHGLLNFQYRVLGAT------ASFRDEKQKPPA----------------AY 185
           Y VR P GK  G L F YR    T       S     Q PP                 + 
Sbjct: 125 YQVRTPFGKGQGSLTFSYRFDSPTFKPDQPVSSHVFHQDPPVSSSHVYTNPMDIPSDFSS 184

Query: 186 ATRGIGPPPE------------GYPP------------------------PPNPAAGYPP 245
           AT    PP              GYPP                        P  P A YPP
Sbjct: 185 ATTNYPPPQSSEANFYPPLSSIGYPPSSPPQHYSSPPYPYPNPYQYHSHYPEQPVAVYPP 244

Query: 246 PPP--------------PPYNTAYAPPP------PQP----YGYP------HYGYPAPPP 262
           PPP              PP + +Y PPP       QP    +G+        +GY  PPP
Sbjct: 245 PPPSASNLYPPPYYSTSPPQHQSYPPPPGHSFHQTQPSQSFHGFAPSSPQNQHGYGYPPP 304

BLAST of MS019277 vs. TAIR 10
Match: AT1G09070.1 (soybean gene regulated by cold-2 )

HSP 1 Score: 89.4 bits (220), Expect = 5.1e-18
Identity = 112/324 (34.57%), Postives = 151/324 (46.60%), Query Frame = 0

Query: 6   SLEINVVSAKDLPEINLVSKMAAYVVVSLTGGGNSDQKFKTNADSSGGINPNWNFPMKFS 65
           SL++ ++SA+DL ++ L+ K   Y VVS+ G   + Q  KT  D   G  P W   MK +
Sbjct: 5   SLDLTIISAEDLKDVQLIGKQDLYAVVSINGDARTKQ--KTKVDKDCGTKPKWKHQMKLT 64

Query: 66  VNELAS-----TLVFTIRCFRTL-GDRDVGDVHVPVADLL-QSTGNRNSMQHVTYPVRKP 125
           V++ A+     TLVF I   R + GD+ VG+V VPV +LL Q+ G+    + VTY VR P
Sbjct: 65  VDDAAARDNRLTLVFEIVADRPIAGDKPVGEVSVPVKELLDQNKGDEE--KTVTYAVRLP 124

Query: 126 SGKTHGLLNFQYR-----VLGATA-------SFRDEK------------QKPPAAYATRG 185
           +GK  G L F ++       G+++       S  D K               P+AY    
Sbjct: 125 NGKAKGSLKFSFKFGEKYTYGSSSGPHAPVPSAMDHKTMDQPVTAYPPGHGAPSAYPAPP 184

Query: 186 IGP----PPEGYPPPPNPAAGYPPPPPPPYNTAYAPPP---PQPYGYPHY------GYPA 245
            GP    PP+G+    +   GYP     P  T   PPP   PQ  GYP Y      GYP 
Sbjct: 185 AGPSSGYPPQGHDDKHDGVYGYPQQAGYPAGTGGYPPPGAYPQQGGYPGYPPQQQGGYPG 244

Query: 246 PPPPPPHYGYHPPPGYLP----------AAGVVAKK----ETKSGMGKILAVGAGILGGL 272
            PP  P YGY P  GY P          A G   K     +  +GMG  L +GAG+LGGL
Sbjct: 245 YPPQGP-YGY-PQQGYPPQGPYGYPQQQAHGKPQKPKKHGKAGAGMGLGLGLGAGLLGGL 304

BLAST of MS019277 vs. TAIR 10
Match: AT4G15740.1 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 79.0 bits (193), Expect = 6.8e-15
Identity = 59/168 (35.12%), Postives = 82/168 (48.81%), Query Frame = 0

Query: 6   SLEINVVSAKDLPEINLVSKMAAYVVVSLTGGGNSD-QKFKTNADSSGGINPNWNFPMKF 65
           +LE+ +VSA D+  I+   KM  Y VVS+ G      Q  KT  D  GG NP WN  +KF
Sbjct: 40  TLELKIVSASDVNHIDATDKMDVYAVVSINGDTTQQKQAAKTPIDYDGGSNPTWNHTVKF 99

Query: 66  SVNELAST---LVFTIRCFR--TLGDRD--VGDVHVPVADLLQS-------TGNRNSMQH 125
           SVNE  +    L  T++ F     GD D  +G+V+V V DLL S        GN N M+ 
Sbjct: 100 SVNEREANEGLLTITVKLFSYWLEGDNDLYLGEVNVSVQDLLASNPIPPFTNGNINKMKS 159

Query: 126 VTYPVRKPSGKTHGLLNFQYRVLGATASFRDEKQKPPAAYATRGIGPP 159
           +T P++     T+  ++  YR          E+  PP+  +   IG P
Sbjct: 160 MTCPIKATEKSTNTTVSLSYRFKPVPV----EEFYPPSPDSLPSIGKP 203

BLAST of MS019277 vs. TAIR 10
Match: AT4G15755.1 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 73.9 bits (180), Expect = 2.2e-13
Identity = 89/287 (31.01%), Postives = 136/287 (47.39%), Query Frame = 0

Query: 6   SLEINVVSAKDLPEINLVSKMAAYVVVSLTG-GGNSDQKFKTNADSSGGINPNWNFPMKF 65
           +LE+N+ SA++L  +NL++KM  +  +++ G      QK KT  D  GG NP WN  +KF
Sbjct: 5   TLELNINSARNLLNVNLITKMNVFTAITINGENTRKKQKAKTTVDRYGGSNPTWNQTIKF 64

Query: 66  SVNELA-----STLVFTIRCFRTLGDRDVGDVHVPVADLLQST-------GNRNSMQHVT 125
           SV+E +     S+LV  +   R LG++++G V++P+ +LL +T       GN + M+ ++
Sbjct: 65  SVDERSARGGHSSLVMRVISRRVLGNKEIGRVNIPLLELLNATTPSFNGDGNDHEMKLMS 124

Query: 126 YPVRKPSGKTHGLLNFQYRVLGATASFRDEKQKPPAAYATRGIGPPPEGYPPPPNPAAGY 185
           Y VR  SGK  G L+F YR         +      AA +     P       PP     +
Sbjct: 125 YQVRTSSGKRSGSLSFSYRFKPNVPVITNRSCVDSAAPSQIEHAP-----SAPPELPIEF 184

Query: 186 PPPPPPPYNTAYAPPP-------PQPYG------------YPHYGYPAPPPPPPHYGYHP 245
           P  P PPY   +           P  YG            Y   G+     PPP  GY  
Sbjct: 185 PKLPQPPYLLRHPFAAGSSRGLLPISYGAGMTEQTGHANNYAQTGHANNYAPPPRQGY-G 244

Query: 246 PPGYLPAA---GVVAKKETKSGMGKILAVGAGILGGLVCGAMLGECA 258
           P GY+ +    G  + ++ K  MG  L +GAG+ GGL+ G ++ + A
Sbjct: 245 PYGYVSSGYGYGSPSYQQRKE-MGIGLGLGAGLFGGLMVGDIVSDVA 284

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022138764.18.7e-12186.23protein SRC2-like [Momordica charantia][more]
KAB1214014.17.8e-4549.45hypothetical protein CJ030_MR5G017277 [Morella rubra][more]
KAA8548920.11.6e-4245.61hypothetical protein F0562_000604 [Nyssa sinensis][more]
KAF3456203.11.4e-4147.97hypothetical protein FNV43_RR00853 [Rhamnella rubrinervis][more]
XP_041000897.11.8e-4147.86protein SRC2-like [Juglans microcarpa x Juglans regia][more]
Match NameE-valueIdentityDescription
O041334.9e-2638.16Protein SRC2 OS=Glycine max OX=3847 GN=SRC2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CB104.2e-12186.23protein SRC2-like OS=Momordica charantia OX=3673 GN=LOC111009847 PE=4 SV=1[more]
A0A2N9IAP92.9e-4547.33C2 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS49360 PE=4 ... [more]
A0A6A1VMH83.8e-4549.45C2 domain-containing protein OS=Morella rubra OX=262757 GN=CJ030_MR5G017277 PE=4... [more]
A0A5J5C4657.8e-4345.61Glutathione transferase OS=Nyssa sinensis OX=561372 GN=F0562_000604 PE=4 SV=1[more]
A0A5B7A4U83.0e-4247.12Putative cytokinesis protein sepA-like OS=Davidia involucrata OX=16924 GN=Din_02... [more]
Match NameE-valueIdentityDescription
AT3G16510.11.5e-2232.49Calcium-dependent lipid-binding (CaLB domain) family protein [more]
AT1G09070.15.1e-1834.57soybean gene regulated by cold-2 [more]
AT4G15740.16.8e-1535.12Calcium-dependent lipid-binding (CaLB domain) family protein [more]
AT4G15755.12.2e-1331.01Calcium-dependent lipid-binding (CaLB domain) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000008C2 domainSMARTSM00239C2_3ccoord: 6..107
e-value: 3.3E-10
score: 49.9
IPR000008C2 domainPFAMPF00168C2coord: 5..107
e-value: 1.8E-14
score: 53.9
IPR000008C2 domainPROSITEPS50004C2coord: 1..108
score: 13.681374
IPR035892C2 domain superfamilyGENE3D2.60.40.150C2 domaincoord: 6..131
e-value: 5.0E-15
score: 57.9
IPR035892C2 domain superfamilySUPERFAMILY49562C2 domain (Calcium/lipid-binding domain, CaLB)coord: 4..133
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 138..187
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 157..187
NoneNo IPR availablePANTHERPTHR32246INGRESSION PROTEIN FIC1coord: 1..260
NoneNo IPR availablePANTHERPTHR32246:SF101OS01G0934100 PROTEINcoord: 1..260
IPR044750SRC2/BAP, C2 domainCDDcd04051C2_SRC2_likecoord: 6..128
e-value: 6.34117E-42
score: 138.518

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS019277.1MS019277.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response