MS019199 (gene) Bitter gourd (TR) v1

Overview
NameMS019199
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionEukaryotic translation initiation factor 3 subunit G
Locationscaffold20: 2468479 .. 2469981 (-)
RNA-Seq ExpressionMS019199
SyntenyMS019199
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTCAGCCAACAAATCCTTCCAAATTGCGATGGGGGGAGCTAGATGACGACGATGGTGATCTTGATTTTCTCCTTCCACCGAAGAAAGTTATTGGGCCAGACGAAAACGGGTTGAAGAAAACTGTTGAGTATCAGTTTAACGAAGATGGTAATAAGGTCAAGATCACAACGACCTCTCGGACTCGCAAGCTTGCGCATGCTCGCCTGAGCAAACATGCAGTGGAGCGCCGATCTTGGGCCAAGTTCGGCGATGCTGTGCATGAAGATGTCGGCAGTAGGCTCACTATGGTCTCCACTGAGGAGATTCTTCTGGAGCGCCCTCGTGCTCCTGGTTTGCTTTCGAAAACTCTTGAATTACGTTGCTTTCGGATTTGCATGTATTTGTTTGCTGTGGCGGCAAGTACTTTTAATTTAGTTATGAAAGTGTGCCATTTGTTTCTATGTGGGAGCTTTGTAGAATTTCCTACAGCTGGAGAAAAAAGGGGTTTTTCTTAAGGTTGCAGTGGTGTTTGAGCTTTAGAAAACTGTGATACTTGATTACTGGAAGTGTGTAAACGTATTTGGTTTACAATTGAAACGAAAAGAGAGAATCGCCTTTAATGGTGTGAAGAGGGTGACATAATTTCCGCAACTTGCATATGGGTTATGTTCTTTTGGGAATTACAGGGTTATGCTTTGAACTGAATACTTAAAATCTTCCATATTATTTTCTCTATATAAACCCCCAAATCCCAATGATATTCTGTTTAAGGGAGATATTAAATCCTAGAGATCTCTCTGTGCCTTTCTTTTGACATTTTGGTTTGTAAATGTGCTTTTTGCACTTCTAATAGGTGATATCGGTGTTGTTTGTTCTCTGTACGGTACACTTTTGATACTCTGCTCACATTAATCCTTTATTGGCGAAATGAAGCATATCTTATTGCCTTGTTACAGTAAGATTTCTTTTAAAGAATGAAAACGATTACAGGTAGCAAACCAGAGGAGCCAAAGGTTGCAGGAGACCCTTTAGCTCAATTGGGAAAGGGTGGTGCTGTTCTTATGGTCTGTCGTACTTGTGGTAAAAAGGGTGACCATTGGACCTCAAGGTGCCCTTACAAGGACCTGGCACCACAAGCTGATGGATTGGATAAGGCTGTTGCACCAGAGGCTACCACCGCTGCTCCTGGTGCAACAAAGGGAACGTATGTTCCTCCTGGTTTGAGACCCGGGGCAGAGAGGACTGGAACTGACATGAGACGTAGGAATGATGAAAACTCTGTTCGTGTGACCAACCTTTCAGAGGACACTAGAGAGCCTGACTTGCTTGAGCTTTTCCGACCTTTCGGTGCTGTTAGCCGAGTTTATGTTGCTGTTGATCAAAAGACTAGCATGAGTAGAGGATTTGGTTTTGTGAACTTTGTGAACCGTGAGGATGCCCAAAGATCAATTAACAAACTCAATGGATATGGTTATGATAATCTCATCCTTCGAGTTGAGTGGGCCACTCCAAGGGCGACT

mRNA sequence

ATGGTTCAGCCAACAAATCCTTCCAAATTGCGATGGGGGGAGCTAGATGACGACGATGGTGATCTTGATTTTCTCCTTCCACCGAAGAAAGTTATTGGGCCAGACGAAAACGGGTTGAAGAAAACTGTTGAGTATCAGTTTAACGAAGATGGTAATAAGGTCAAGATCACAACGACCTCTCGGACTCGCAAGCTTGCGCATGCTCGCCTGAGCAAACATGCAGTGGAGCGCCGATCTTGGGCCAAGTTCGGCGATGCTGTGCATGAAGATGTCGGCAGTAGGCTCACTATGGTCTCCACTGAGGAGATTCTTCTGGAGCGCCCTCGTGCTCCTGGTAGCAAACCAGAGGAGCCAAAGGTTGCAGGAGACCCTTTAGCTCAATTGGGAAAGGGTGGTGCTGTTCTTATGGTCTGTCGTACTTGTGGTAAAAAGGGTGACCATTGGACCTCAAGGTGCCCTTACAAGGACCTGGCACCACAAGCTGATGGATTGGATAAGGCTGTTGCACCAGAGGCTACCACCGCTGCTCCTGGTGCAACAAAGGGAACGTATGTTCCTCCTGGTTTGAGACCCGGGGCAGAGAGGACTGGAACTGACATGAGACGTAGGAATGATGAAAACTCTGTTCGTGTGACCAACCTTTCAGAGGACACTAGAGAGCCTGACTTGCTTGAGCTTTTCCGACCTTTCGGTGCTGTTAGCCGAGTTTATGTTGCTGTTGATCAAAAGACTAGCATGAGTAGAGGATTTGGTTTTGTGAACTTTGTGAACCGTGAGGATGCCCAAAGATCAATTAACAAACTCAATGGATATGGTTATGATAATCTCATCCTTCGAGTTGAGTGGGCCACTCCAAGGGCGACT

Coding sequence (CDS)

ATGGTTCAGCCAACAAATCCTTCCAAATTGCGATGGGGGGAGCTAGATGACGACGATGGTGATCTTGATTTTCTCCTTCCACCGAAGAAAGTTATTGGGCCAGACGAAAACGGGTTGAAGAAAACTGTTGAGTATCAGTTTAACGAAGATGGTAATAAGGTCAAGATCACAACGACCTCTCGGACTCGCAAGCTTGCGCATGCTCGCCTGAGCAAACATGCAGTGGAGCGCCGATCTTGGGCCAAGTTCGGCGATGCTGTGCATGAAGATGTCGGCAGTAGGCTCACTATGGTCTCCACTGAGGAGATTCTTCTGGAGCGCCCTCGTGCTCCTGGTAGCAAACCAGAGGAGCCAAAGGTTGCAGGAGACCCTTTAGCTCAATTGGGAAAGGGTGGTGCTGTTCTTATGGTCTGTCGTACTTGTGGTAAAAAGGGTGACCATTGGACCTCAAGGTGCCCTTACAAGGACCTGGCACCACAAGCTGATGGATTGGATAAGGCTGTTGCACCAGAGGCTACCACCGCTGCTCCTGGTGCAACAAAGGGAACGTATGTTCCTCCTGGTTTGAGACCCGGGGCAGAGAGGACTGGAACTGACATGAGACGTAGGAATGATGAAAACTCTGTTCGTGTGACCAACCTTTCAGAGGACACTAGAGAGCCTGACTTGCTTGAGCTTTTCCGACCTTTCGGTGCTGTTAGCCGAGTTTATGTTGCTGTTGATCAAAAGACTAGCATGAGTAGAGGATTTGGTTTTGTGAACTTTGTGAACCGTGAGGATGCCCAAAGATCAATTAACAAACTCAATGGATATGGTTATGATAATCTCATCCTTCGAGTTGAGTGGGCCACTCCAAGGGCGACT

Protein sequence

MVQPTNPSKLRWGELDDDDGDLDFLLPPKKVIGPDENGLKKTVEYQFNEDGNKVKITTTSRTRKLAHARLSKHAVERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKVAGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLAPQADGLDKAVAPEATTAAPGATKGTYVPPGLRPGAERTGTDMRRRNDENSVRVTNLSEDTREPDLLELFRPFGAVSRVYVAVDQKTSMSRGFGFVNFVNREDAQRSINKLNGYGYDNLILRVEWATPRAT
Homology
BLAST of MS019199 vs. NCBI nr
Match: XP_022143427.1 (eukaryotic translation initiation factor 3 subunit G-like [Momordica charantia])

HSP 1 Score: 584.7 bits (1506), Expect = 4.3e-163
Identity = 287/288 (99.65%), Postives = 287/288 (99.65%), Query Frame = 0

Query: 1   MVQPTNPSKLRWGELDDDDGDLDFLLPPKKVIGPDENGLKKTVEYQFNEDGNKVKITTTS 60
           MVQPTNPSKLRWGELDDDD DLDFLLPPKKVIGPDENGLKKTVEYQFNEDGNKVKITTTS
Sbjct: 1   MVQPTNPSKLRWGELDDDDSDLDFLLPPKKVIGPDENGLKKTVEYQFNEDGNKVKITTTS 60

Query: 61  RTRKLAHARLSKHAVERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV 120
           RTRKLAHARLSKHAVERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV
Sbjct: 61  RTRKLAHARLSKHAVERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV 120

Query: 121 AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLAPQADGLDKAVAPEATTAAPGAT 180
           AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLAPQADGLDKAVAPEATTAAPGAT
Sbjct: 121 AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLAPQADGLDKAVAPEATTAAPGAT 180

Query: 181 KGTYVPPGLRPGAERTGTDMRRRNDENSVRVTNLSEDTREPDLLELFRPFGAVSRVYVAV 240
           KGTYVPPGLRPGAERTGTDMRRRNDENSVRVTNLSEDTREPDLLELFRPFGAVSRVYVAV
Sbjct: 181 KGTYVPPGLRPGAERTGTDMRRRNDENSVRVTNLSEDTREPDLLELFRPFGAVSRVYVAV 240

Query: 241 DQKTSMSRGFGFVNFVNREDAQRSINKLNGYGYDNLILRVEWATPRAT 289
           DQKTSMSRGFGFVNFVNREDAQRSINKLNGYGYDNLILRVEWATPRAT
Sbjct: 241 DQKTSMSRGFGFVNFVNREDAQRSINKLNGYGYDNLILRVEWATPRAT 288

BLAST of MS019199 vs. NCBI nr
Match: XP_022949274.1 (eukaryotic translation initiation factor 3 subunit G-like [Cucurbita moschata] >XP_022998190.1 eukaryotic translation initiation factor 3 subunit G-like [Cucurbita maxima] >XP_023525479.1 eukaryotic translation initiation factor 3 subunit G-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 568.9 bits (1465), Expect = 2.4e-158
Identity = 277/288 (96.18%), Postives = 283/288 (98.26%), Query Frame = 0

Query: 1   MVQPTNPSKLRWGELDDDDGDLDFLLPPKKVIGPDENGLKKTVEYQFNEDGNKVKITTTS 60
           MVQPTNPSKLRWGELDDDDGDLDFLLPPK+VIGPDENGLKKT+EYQFNEDGNKVKITTTS
Sbjct: 1   MVQPTNPSKLRWGELDDDDGDLDFLLPPKQVIGPDENGLKKTIEYQFNEDGNKVKITTTS 60

Query: 61  RTRKLAHARLSKHAVERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV 120
           RTRKLA+ARLSKHA+ERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV
Sbjct: 61  RTRKLANARLSKHAIERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV 120

Query: 121 AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLAPQADGLDKAVAPEATTAAPGAT 180
           AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLA QADGLDK VAPEATTAAPGAT
Sbjct: 121 AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLASQADGLDKTVAPEATTAAPGAT 180

Query: 181 KGTYVPPGLRPGAERTGTDMRRRNDENSVRVTNLSEDTREPDLLELFRPFGAVSRVYVAV 240
           KG YVPPG+R GAERTGTDMRRRNDENSVRVTNLSEDTREPDLLELFRPFGAVSRVYVAV
Sbjct: 181 KGAYVPPGMRAGAERTGTDMRRRNDENSVRVTNLSEDTREPDLLELFRPFGAVSRVYVAV 240

Query: 241 DQKTSMSRGFGFVNFVNREDAQRSINKLNGYGYDNLILRVEWATPRAT 289
           DQKT MSRGFGFVNFVNREDAQR+INKLNGYGYDNLILRVEWATPRAT
Sbjct: 241 DQKTGMSRGFGFVNFVNREDAQRAINKLNGYGYDNLILRVEWATPRAT 288

BLAST of MS019199 vs. NCBI nr
Match: KAG6607041.1 (Eukaryotic translation initiation factor 3 subunit G, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036741.1 Eukaryotic translation initiation factor 3 subunit G [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 567.0 bits (1460), Expect = 9.3e-158
Identity = 276/288 (95.83%), Postives = 282/288 (97.92%), Query Frame = 0

Query: 1   MVQPTNPSKLRWGELDDDDGDLDFLLPPKKVIGPDENGLKKTVEYQFNEDGNKVKITTTS 60
           MVQPTNPSKLRWGELDDDDGDLDFLLPPK+VIGPDENGLKKT+EYQFNEDGNKVKITTTS
Sbjct: 1   MVQPTNPSKLRWGELDDDDGDLDFLLPPKQVIGPDENGLKKTIEYQFNEDGNKVKITTTS 60

Query: 61  RTRKLAHARLSKHAVERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV 120
           RTRKLA+ARLSKHA+ERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV
Sbjct: 61  RTRKLANARLSKHAIERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV 120

Query: 121 AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLAPQADGLDKAVAPEATTAAPGAT 180
           AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLA QADGLDK VAPEAT AAPGAT
Sbjct: 121 AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLASQADGLDKTVAPEATAAAPGAT 180

Query: 181 KGTYVPPGLRPGAERTGTDMRRRNDENSVRVTNLSEDTREPDLLELFRPFGAVSRVYVAV 240
           KG YVPPG+R GAERTGTDMRRRNDENSVRVTNLSEDTREPDLLELFRPFGAVSRVYVAV
Sbjct: 181 KGAYVPPGMRAGAERTGTDMRRRNDENSVRVTNLSEDTREPDLLELFRPFGAVSRVYVAV 240

Query: 241 DQKTSMSRGFGFVNFVNREDAQRSINKLNGYGYDNLILRVEWATPRAT 289
           DQKT MSRGFGFVNFVNREDAQR+INKLNGYGYDNLILRVEWATPRAT
Sbjct: 241 DQKTGMSRGFGFVNFVNREDAQRAINKLNGYGYDNLILRVEWATPRAT 288

BLAST of MS019199 vs. NCBI nr
Match: XP_038876811.1 (eukaryotic translation initiation factor 3 subunit G-like [Benincasa hispida])

HSP 1 Score: 564.7 bits (1454), Expect = 4.6e-157
Identity = 276/288 (95.83%), Postives = 281/288 (97.57%), Query Frame = 0

Query: 1   MVQPTNPSKLRWGELDDDDGDLDFLLPPKKVIGPDENGLKKTVEYQFNEDGNKVKITTTS 60
           MVQPTN SKLRWGEL+DDDGDLDFLLPPK++IGPDENGLKK VEYQFNEDGNKVKITTT+
Sbjct: 1   MVQPTNTSKLRWGELEDDDGDLDFLLPPKQIIGPDENGLKKIVEYQFNEDGNKVKITTTT 60

Query: 61  RTRKLAHARLSKHAVERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV 120
           RTRKLAHARLSKHAVERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV
Sbjct: 61  RTRKLAHARLSKHAVERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV 120

Query: 121 AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLAPQADGLDKAVAPEATTAAPGAT 180
           AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLAPQADGLDK VAPEATTAAPGAT
Sbjct: 121 AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLAPQADGLDKPVAPEATTAAPGAT 180

Query: 181 KGTYVPPGLRPGAERTGTDMRRRNDENSVRVTNLSEDTREPDLLELFRPFGAVSRVYVAV 240
           KG YVPPG+R GAERTGTDMRRRNDENSVRVTNLSEDTREPDLLELFR FGAVSRVYVAV
Sbjct: 181 KGAYVPPGMRAGAERTGTDMRRRNDENSVRVTNLSEDTREPDLLELFRSFGAVSRVYVAV 240

Query: 241 DQKTSMSRGFGFVNFVNREDAQRSINKLNGYGYDNLILRVEWATPRAT 289
           DQKT MSRGFGFVNFVNREDAQRSINKLNGYGYDNLILRVEWATPRAT
Sbjct: 241 DQKTGMSRGFGFVNFVNREDAQRSINKLNGYGYDNLILRVEWATPRAT 288

BLAST of MS019199 vs. NCBI nr
Match: XP_022935522.1 (eukaryotic translation initiation factor 3 subunit G-like [Cucurbita moschata] >KAG6581171.1 Eukaryotic translation initiation factor 3 subunit G, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017908.1 Eukaryotic translation initiation factor 3 subunit G [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 563.1 bits (1450), Expect = 1.3e-156
Identity = 275/288 (95.49%), Postives = 280/288 (97.22%), Query Frame = 0

Query: 1   MVQPTNPSKLRWGELDDDDGDLDFLLPPKKVIGPDENGLKKTVEYQFNEDGNKVKITTTS 60
           MVQ TNPSKLRWGEL+DDDGDLDFLLPPK++IGPDENGLKK VEYQFNEDGNKVKITTT+
Sbjct: 1   MVQQTNPSKLRWGELEDDDGDLDFLLPPKQIIGPDENGLKKIVEYQFNEDGNKVKITTTT 60

Query: 61  RTRKLAHARLSKHAVERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV 120
           RTRKLAHARLSKHAVERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV
Sbjct: 61  RTRKLAHARLSKHAVERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV 120

Query: 121 AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLAPQADGLDKAVAPEATTAAPGAT 180
           AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLAPQADGLDK VAPEA TAAPGAT
Sbjct: 121 AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLAPQADGLDKPVAPEAATAAPGAT 180

Query: 181 KGTYVPPGLRPGAERTGTDMRRRNDENSVRVTNLSEDTREPDLLELFRPFGAVSRVYVAV 240
           KG YVPPG+R GAERTGTDMRRRNDENSVRVTNLSEDTREPDL ELFRPFGAVSRVYVAV
Sbjct: 181 KGAYVPPGMRAGAERTGTDMRRRNDENSVRVTNLSEDTREPDLHELFRPFGAVSRVYVAV 240

Query: 241 DQKTSMSRGFGFVNFVNREDAQRSINKLNGYGYDNLILRVEWATPRAT 289
           DQKT MSRGFGFVNFVNREDAQRSINKLNGYGYDNLILRVEWATPRAT
Sbjct: 241 DQKTGMSRGFGFVNFVNREDAQRSINKLNGYGYDNLILRVEWATPRAT 288

BLAST of MS019199 vs. ExPASy Swiss-Prot
Match: Q6DRC4 (Eukaryotic translation initiation factor 3 subunit G OS=Danio rerio OX=7955 GN=eif3g PE=1 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 3.3e-49
Identity = 117/293 (39.93%), Postives = 163/293 (55.63%), Query Frame = 0

Query: 8   SKLRWGELDDDDGDLDFLLPPKKVIGPDENGLKKTVEYQFNEDGNKVKITTTSRTRKLAH 67
           SK  W +  +++GD   L  PK+ +   +  +K   EY+ ++DG K KI    RT K+  
Sbjct: 9   SKPSWADQVEEEGDEGSLPSPKETV---KGNIKTVTEYKIDDDGQKFKII---RTFKIET 68

Query: 68  ARLSKHAVERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKVAGDPLAQ 127
            + SK    R++W KFG++ ++  G  +   +  + +     +            DP+ +
Sbjct: 69  RKASKAVARRKNWKKFGNSEYDAPGPNVATTTVSDDVFMTFISSKEDLNAQDQDEDPMNK 128

Query: 128 LGKGGAVLMVCRTCGKKGDHWTSRCPYKD-LAPQADGL-----------DKAVAPEATTA 187
           L   G  ++ CR C  KGDHWT+RCPYKD L P    L           +KA  PE    
Sbjct: 129 L--KGQKIVSCRIC--KGDHWTTRCPYKDTLGPMQKELAEQLGLSTGEKEKAAEPEPAQP 188

Query: 188 APGATKGTYVPPGLRPGAERTGTDM---RRRNDENSVRVTNLSEDTREPDLLELFRPFGA 247
               T G YVPP LR G  R G  M   RR +D  ++RVTNLSEDTRE DL ELFRPFG+
Sbjct: 189 VQNKT-GKYVPPSLRDGGTRRGESMQPNRRADDNATIRVTNLSEDTRETDLQELFRPFGS 248

Query: 248 VSRVYVAVDQKTSMSRGFGFVNFVNREDAQRSINKLNGYGYDNLILRVEWATP 286
           +SR+Y+A D+ T  S+GF F++F  REDA R+I  ++G+GYD+LIL VEWA P
Sbjct: 249 ISRIYLAKDKNTGQSKGFAFISFHRREDAARAIAGVSGFGYDHLILNVEWAKP 290

BLAST of MS019199 vs. ExPASy Swiss-Prot
Match: A7SKE9 (Eukaryotic translation initiation factor 3 subunit G OS=Nematostella vectensis OX=45351 GN=v1g171563 PE=3 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 2.8e-48
Identity = 121/295 (41.02%), Postives = 167/295 (56.61%), Query Frame = 0

Query: 9   KLRWGELDDDDGD--LDFLLPPKKVIGPDENGLKKTVEYQFNEDGNKVKITTTSRTRKLA 68
           K  W +L D+ G+  L   +PP   +   EN  K   EY+ +++G   KI TT R     
Sbjct: 11  KPSWADLVDEAGETGLTGQIPPSSEVREGEN--KIVTEYKRDDEGKLQKIITTYRVET-- 70

Query: 69  HARLSKHAVERRSWAKFGDAVHEDVGSRLTMVSTEE----ILLERPRAPGSKPEEPKVAG 128
             R++K   +R+ W KFGD+ ++  G   T  +  +    IL      P +  E      
Sbjct: 71  -RRVAKEIAKRKLWKKFGDSKNDKPGPNKTTTNVCDDVFLILTSNKENPDATEE------ 130

Query: 129 DPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKD-------LAPQADGLDKAVAPEATTA 188
           DPL +L   G  ++ CR C  KGDHWT++CPYKD          + DG  +   P +  A
Sbjct: 131 DPLKKL--SGQKIVQCRIC--KGDHWTTKCPYKDQLEVIHQQLKEDDGSGQPGTPPSEPA 190

Query: 189 APGA--TKGTYVPPGLRPGAERTGTDM---RRRNDENSVRVTNLSEDTREPDLLELFRPF 248
            P A  T G YV PGLR GA R G  M    +R++  ++RVTNLSE+TRE DL ELFRP 
Sbjct: 191 NPAAASTSGKYVAPGLRDGANRRGETMPSRSQRDETATIRVTNLSEETRESDLQELFRPL 250

Query: 249 GAVSRVYVAVDQKTSMSRGFGFVNFVNREDAQRSINKLNGYGYDNLILRVEWATP 286
           G +SR+++A D+ T+ S+GF F+NFV+REDA R+I  L+G+GYD+LIL VEWA P
Sbjct: 251 GPISRIFLAKDKFTNQSKGFAFINFVHREDAARAIEVLSGFGYDHLILNVEWAKP 290

BLAST of MS019199 vs. ExPASy Swiss-Prot
Match: Q28CY2 (Eukaryotic translation initiation factor 3 subunit G OS=Xenopus tropicalis OX=8364 GN=eif3g PE=2 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 2.8e-48
Identity = 126/310 (40.65%), Postives = 174/310 (56.13%), Query Frame = 0

Query: 8   SKLRWGELDDDDG-DLDFLLPPKKVIGPDE------------NGLKKTV-EYQFNEDGNK 67
           SK  W +  +++G D + + PP   +  D             NG  KT+ EY+ N++G K
Sbjct: 8   SKPSWADQVEEEGIDPEPISPPVTKVQQDPASFVLDTPQEVINGKIKTITEYKLNDEGKK 67

Query: 68  VKITTTSRTRKLAHARLSKHAVERRSWAKFGDAVHEDVGSRL--TMVSTEEILLERPRAP 127
           +KI    RT K+   + SK    R++W KFG++ ++  G  +  T VS + ++       
Sbjct: 68  IKIV---RTFKIETLKASKVVAHRKNWKKFGNSEYDPPGPNVATTTVSDDVLMTFITSKE 127

Query: 128 GSKPEEPKVAGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKD-LAPQADGL------ 187
               +E +   DP+ +L   G  ++ CR C  KGDHWT+RCPYKD L P    L      
Sbjct: 128 DLNNQEEE---DPMNKL--KGQKIVSCRIC--KGDHWTTRCPYKDTLGPMQKELAEQLGL 187

Query: 188 ---DKAVAPEA---TTAAPGATKGTYVPPGLRPGAERTGTDM---RRRNDENSVRVTNLS 247
              DK  AP A      AP +  G YVPP LR G  R G  M   RR +D  ++RVTNLS
Sbjct: 188 STGDKEKAPGAEPEPAQAPVSKTGKYVPPSLRDGGSRRGESMQPNRRADDNATIRVTNLS 247

Query: 248 EDTREPDLLELFRPFGAVSRVYVAVDQKTSMSRGFGFVNFVNREDAQRSINKLNGYGYDN 286
           EDTRE DL ELFRPFG++SR+Y+A D+ T  S+GF F++F  REDA R+I  ++G+GYD+
Sbjct: 248 EDTRETDLQELFRPFGSISRIYLAKDKTTGQSKGFAFISFHRREDAARAIAGVSGFGYDH 307

BLAST of MS019199 vs. ExPASy Swiss-Prot
Match: Q6DJI8 (Eukaryotic translation initiation factor 3 subunit G-A OS=Xenopus laevis OX=8355 GN=eif3g-a PE=2 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 1.4e-47
Identity = 121/277 (43.68%), Postives = 163/277 (58.84%), Query Frame = 0

Query: 28  PKKVIGPDENGLKKTV-EYQFNEDGNKVKITTTSRTRKLAHARLSKHAVERRSWAKFGDA 87
           P++VI    NG  KT+ EY+ N+D   VKI    RT K+   + SK   +R++W KFG++
Sbjct: 43  PREVI----NGNIKTITEYKLNDDDKTVKIV---RTFKIETVKASKVVAQRKNWKKFGNS 102

Query: 88  VHEDVGSRL--TMVSTEEILLERPRAPGSKPEEPKVAGDPLAQLGKGGAVLMVCRTCGKK 147
            ++  G  +  T VS + ++           +E +   DP+ +L   G  ++ CR C  K
Sbjct: 103 EYDPPGPNVATTTVSDDVLMTFITNKEDLNNQEEE---DPMNKL--KGQKIVSCRIC--K 162

Query: 148 GDHWTSRCPYKD-LAPQADGL---------DKAVAPEA---TTAAPGATKGTYVPPGLRP 207
           GDHWT+RCPYKD L P    L         DK  AP A      AP +  G YVPP LR 
Sbjct: 163 GDHWTTRCPYKDTLGPMQKELAEQLGLSTGDKEKAPGAEPEPAQAPVSKTGKYVPPSLRD 222

Query: 208 GAERTGTDM---RRRNDENSVRVTNLSEDTREPDLLELFRPFGAVSRVYVAVDQKTSMSR 267
           G  R G  M   RR +D  ++RVTNLSEDTRE DL ELFRPFG++SR+Y+A D+ T  S+
Sbjct: 223 GGSRRGESMQPNRRADDNATIRVTNLSEDTRETDLQELFRPFGSISRIYLAKDKTTGQSK 282

Query: 268 GFGFVNFVNREDAQRSINKLNGYGYDNLILRVEWATP 286
           GF F++F  REDA R+I  ++G+GYD+LIL VEWA P
Sbjct: 283 GFAFISFHRREDAARAIAGVSGFGYDHLILNVEWAKP 305

BLAST of MS019199 vs. ExPASy Swiss-Prot
Match: Q641B2 (Eukaryotic translation initiation factor 3 subunit G-B OS=Xenopus laevis OX=8355 GN=eif3g-b PE=2 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 3.1e-47
Identity = 126/308 (40.91%), Postives = 172/308 (55.84%), Query Frame = 0

Query: 8   SKLRWGELDDDDG-DLDFLLPPKKVIGPDE----------NGLKKTV-EYQFNEDGNKVK 67
           SK  W +  +++G D++ L P  K   P            NG  KT+ EY+ N+   K+K
Sbjct: 8   SKPSWADQVEEEGIDVEPLSPQIKKQDPASFVLDTPREVINGNIKTITEYKLNDKDKKIK 67

Query: 68  ITTTSRTRKLAHARLSKHAVERRSWAKFGDAVHEDVGSRL--TMVSTEEILLERPRAPGS 127
           I    RT K+   + SK    R++W KFG++ ++  G  +  T VS + ++         
Sbjct: 68  IV---RTFKIETLKASKVVAHRKNWKKFGNSEYDPPGPNVATTTVSDDVLMTFITNKEDL 127

Query: 128 KPEEPKVAGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKD-LAPQADGL-------- 187
             +E +   DP+ +L   G  ++ CR C  KGDHWT+RCPYKD L P    L        
Sbjct: 128 NNQEEE---DPMNKL--KGQKIVSCRIC--KGDHWTTRCPYKDTLGPMQKELAEQLGLST 187

Query: 188 -DKAVAPEA---TTAAPGATKGTYVPPGLRPGAERTGTDM---RRRNDENSVRVTNLSED 247
            DK  AP A      AP +  G YVPP LR G  R G  M   RR +D  ++RVTNLSED
Sbjct: 188 ADKEKAPGAEPEPAQAPVSKTGKYVPPSLRDGGSRRGESMQPNRRADDNATIRVTNLSED 247

Query: 248 TREPDLLELFRPFGAVSRVYVAVDQKTSMSRGFGFVNFVNREDAQRSINKLNGYGYDNLI 286
           TRE DL ELFRPFG++SR+Y+A D+ T  S+GF F++F  REDA R+I  ++G+GYD+LI
Sbjct: 248 TRETDLQELFRPFGSISRIYLAKDKTTGQSKGFAFISFHRREDAARAIAGVSGFGYDHLI 305

BLAST of MS019199 vs. ExPASy TrEMBL
Match: A0A6J1CQS9 (Eukaryotic translation initiation factor 3 subunit G OS=Momordica charantia OX=3673 GN=LOC111013305 PE=3 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 2.1e-163
Identity = 287/288 (99.65%), Postives = 287/288 (99.65%), Query Frame = 0

Query: 1   MVQPTNPSKLRWGELDDDDGDLDFLLPPKKVIGPDENGLKKTVEYQFNEDGNKVKITTTS 60
           MVQPTNPSKLRWGELDDDD DLDFLLPPKKVIGPDENGLKKTVEYQFNEDGNKVKITTTS
Sbjct: 1   MVQPTNPSKLRWGELDDDDSDLDFLLPPKKVIGPDENGLKKTVEYQFNEDGNKVKITTTS 60

Query: 61  RTRKLAHARLSKHAVERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV 120
           RTRKLAHARLSKHAVERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV
Sbjct: 61  RTRKLAHARLSKHAVERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV 120

Query: 121 AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLAPQADGLDKAVAPEATTAAPGAT 180
           AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLAPQADGLDKAVAPEATTAAPGAT
Sbjct: 121 AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLAPQADGLDKAVAPEATTAAPGAT 180

Query: 181 KGTYVPPGLRPGAERTGTDMRRRNDENSVRVTNLSEDTREPDLLELFRPFGAVSRVYVAV 240
           KGTYVPPGLRPGAERTGTDMRRRNDENSVRVTNLSEDTREPDLLELFRPFGAVSRVYVAV
Sbjct: 181 KGTYVPPGLRPGAERTGTDMRRRNDENSVRVTNLSEDTREPDLLELFRPFGAVSRVYVAV 240

Query: 241 DQKTSMSRGFGFVNFVNREDAQRSINKLNGYGYDNLILRVEWATPRAT 289
           DQKTSMSRGFGFVNFVNREDAQRSINKLNGYGYDNLILRVEWATPRAT
Sbjct: 241 DQKTSMSRGFGFVNFVNREDAQRSINKLNGYGYDNLILRVEWATPRAT 288

BLAST of MS019199 vs. ExPASy TrEMBL
Match: A0A6J1KBW4 (Eukaryotic translation initiation factor 3 subunit G OS=Cucurbita maxima OX=3661 GN=LOC111492915 PE=3 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 1.2e-158
Identity = 277/288 (96.18%), Postives = 283/288 (98.26%), Query Frame = 0

Query: 1   MVQPTNPSKLRWGELDDDDGDLDFLLPPKKVIGPDENGLKKTVEYQFNEDGNKVKITTTS 60
           MVQPTNPSKLRWGELDDDDGDLDFLLPPK+VIGPDENGLKKT+EYQFNEDGNKVKITTTS
Sbjct: 1   MVQPTNPSKLRWGELDDDDGDLDFLLPPKQVIGPDENGLKKTIEYQFNEDGNKVKITTTS 60

Query: 61  RTRKLAHARLSKHAVERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV 120
           RTRKLA+ARLSKHA+ERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV
Sbjct: 61  RTRKLANARLSKHAIERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV 120

Query: 121 AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLAPQADGLDKAVAPEATTAAPGAT 180
           AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLA QADGLDK VAPEATTAAPGAT
Sbjct: 121 AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLASQADGLDKTVAPEATTAAPGAT 180

Query: 181 KGTYVPPGLRPGAERTGTDMRRRNDENSVRVTNLSEDTREPDLLELFRPFGAVSRVYVAV 240
           KG YVPPG+R GAERTGTDMRRRNDENSVRVTNLSEDTREPDLLELFRPFGAVSRVYVAV
Sbjct: 181 KGAYVPPGMRAGAERTGTDMRRRNDENSVRVTNLSEDTREPDLLELFRPFGAVSRVYVAV 240

Query: 241 DQKTSMSRGFGFVNFVNREDAQRSINKLNGYGYDNLILRVEWATPRAT 289
           DQKT MSRGFGFVNFVNREDAQR+INKLNGYGYDNLILRVEWATPRAT
Sbjct: 241 DQKTGMSRGFGFVNFVNREDAQRAINKLNGYGYDNLILRVEWATPRAT 288

BLAST of MS019199 vs. ExPASy TrEMBL
Match: A0A6J1GCD4 (Eukaryotic translation initiation factor 3 subunit G OS=Cucurbita moschata OX=3662 GN=LOC111452682 PE=3 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 1.2e-158
Identity = 277/288 (96.18%), Postives = 283/288 (98.26%), Query Frame = 0

Query: 1   MVQPTNPSKLRWGELDDDDGDLDFLLPPKKVIGPDENGLKKTVEYQFNEDGNKVKITTTS 60
           MVQPTNPSKLRWGELDDDDGDLDFLLPPK+VIGPDENGLKKT+EYQFNEDGNKVKITTTS
Sbjct: 1   MVQPTNPSKLRWGELDDDDGDLDFLLPPKQVIGPDENGLKKTIEYQFNEDGNKVKITTTS 60

Query: 61  RTRKLAHARLSKHAVERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV 120
           RTRKLA+ARLSKHA+ERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV
Sbjct: 61  RTRKLANARLSKHAIERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV 120

Query: 121 AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLAPQADGLDKAVAPEATTAAPGAT 180
           AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLA QADGLDK VAPEATTAAPGAT
Sbjct: 121 AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLASQADGLDKTVAPEATTAAPGAT 180

Query: 181 KGTYVPPGLRPGAERTGTDMRRRNDENSVRVTNLSEDTREPDLLELFRPFGAVSRVYVAV 240
           KG YVPPG+R GAERTGTDMRRRNDENSVRVTNLSEDTREPDLLELFRPFGAVSRVYVAV
Sbjct: 181 KGAYVPPGMRAGAERTGTDMRRRNDENSVRVTNLSEDTREPDLLELFRPFGAVSRVYVAV 240

Query: 241 DQKTSMSRGFGFVNFVNREDAQRSINKLNGYGYDNLILRVEWATPRAT 289
           DQKT MSRGFGFVNFVNREDAQR+INKLNGYGYDNLILRVEWATPRAT
Sbjct: 241 DQKTGMSRGFGFVNFVNREDAQRAINKLNGYGYDNLILRVEWATPRAT 288

BLAST of MS019199 vs. ExPASy TrEMBL
Match: A0A6J1F5T2 (Eukaryotic translation initiation factor 3 subunit G OS=Cucurbita moschata OX=3662 GN=LOC111442372 PE=3 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 6.5e-157
Identity = 275/288 (95.49%), Postives = 280/288 (97.22%), Query Frame = 0

Query: 1   MVQPTNPSKLRWGELDDDDGDLDFLLPPKKVIGPDENGLKKTVEYQFNEDGNKVKITTTS 60
           MVQ TNPSKLRWGEL+DDDGDLDFLLPPK++IGPDENGLKK VEYQFNEDGNKVKITTT+
Sbjct: 1   MVQQTNPSKLRWGELEDDDGDLDFLLPPKQIIGPDENGLKKIVEYQFNEDGNKVKITTTT 60

Query: 61  RTRKLAHARLSKHAVERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV 120
           RTRKLAHARLSKHAVERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV
Sbjct: 61  RTRKLAHARLSKHAVERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV 120

Query: 121 AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLAPQADGLDKAVAPEATTAAPGAT 180
           AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLAPQADGLDK VAPEA TAAPGAT
Sbjct: 121 AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLAPQADGLDKPVAPEAATAAPGAT 180

Query: 181 KGTYVPPGLRPGAERTGTDMRRRNDENSVRVTNLSEDTREPDLLELFRPFGAVSRVYVAV 240
           KG YVPPG+R GAERTGTDMRRRNDENSVRVTNLSEDTREPDL ELFRPFGAVSRVYVAV
Sbjct: 181 KGAYVPPGMRAGAERTGTDMRRRNDENSVRVTNLSEDTREPDLHELFRPFGAVSRVYVAV 240

Query: 241 DQKTSMSRGFGFVNFVNREDAQRSINKLNGYGYDNLILRVEWATPRAT 289
           DQKT MSRGFGFVNFVNREDAQRSINKLNGYGYDNLILRVEWATPRAT
Sbjct: 241 DQKTGMSRGFGFVNFVNREDAQRSINKLNGYGYDNLILRVEWATPRAT 288

BLAST of MS019199 vs. ExPASy TrEMBL
Match: A0A6J1J7Q0 (Eukaryotic translation initiation factor 3 subunit G OS=Cucurbita maxima OX=3661 GN=LOC111482111 PE=3 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 1.4e-156
Identity = 274/288 (95.14%), Postives = 280/288 (97.22%), Query Frame = 0

Query: 1   MVQPTNPSKLRWGELDDDDGDLDFLLPPKKVIGPDENGLKKTVEYQFNEDGNKVKITTTS 60
           MVQ TNPSKLRWGEL+DDDGDLDFLLPPK++IGPDENGLKK VEYQFNEDGNKVKITTT+
Sbjct: 1   MVQQTNPSKLRWGELEDDDGDLDFLLPPKQIIGPDENGLKKIVEYQFNEDGNKVKITTTT 60

Query: 61  RTRKLAHARLSKHAVERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV 120
           RTRKLAHARLSKHAVERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV
Sbjct: 61  RTRKLAHARLSKHAVERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKV 120

Query: 121 AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLAPQADGLDKAVAPEATTAAPGAT 180
           AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLAPQADGLDK VAPEA TAAPGAT
Sbjct: 121 AGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLAPQADGLDKPVAPEAGTAAPGAT 180

Query: 181 KGTYVPPGLRPGAERTGTDMRRRNDENSVRVTNLSEDTREPDLLELFRPFGAVSRVYVAV 240
           KG YVPPG+R GAERTGTDMRRRNDENSVRVTNLSEDTREPDL ELFRPFGAVSRVYVA+
Sbjct: 181 KGAYVPPGMRAGAERTGTDMRRRNDENSVRVTNLSEDTREPDLQELFRPFGAVSRVYVAI 240

Query: 241 DQKTSMSRGFGFVNFVNREDAQRSINKLNGYGYDNLILRVEWATPRAT 289
           DQKT MSRGFGFVNFVNREDAQRSINKLNGYGYDNLILRVEWATPRAT
Sbjct: 241 DQKTCMSRGFGFVNFVNREDAQRSINKLNGYGYDNLILRVEWATPRAT 288

BLAST of MS019199 vs. TAIR 10
Match: AT3G11400.1 (eukaryotic translation initiation factor 3G1 )

HSP 1 Score: 429.1 bits (1102), Expect = 2.8e-120
Identity = 214/286 (74.83%), Postives = 245/286 (85.66%), Query Frame = 0

Query: 8   SKLRWGELDDDDGDLDFLLPPKKVIGPDENGLKKTVEYQFNEDGNKVKITTTSRTRKLAH 67
           SK RWGE+D+D+ DLDFLLPPK+VIGPDENGLK T+EY+FN++ NKVKITT +R RKLA 
Sbjct: 10  SKFRWGEMDEDE-DLDFLLPPKQVIGPDENGLKTTIEYKFNDEENKVKITTRTRVRKLAS 69

Query: 68  ARLSKHAVERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKVAGDPLAQ 127
           ARL+K A+ERR+W KFGDA +E+ GS LTMVSTEEILLERPRAPG+K +E K  GD L+Q
Sbjct: 70  ARLNKRAMERRNWPKFGDAANEEAGSHLTMVSTEEILLERPRAPGTKADESKATGDGLSQ 129

Query: 128 LGKGGAVLMVCRTCGKKGDHWTSRCPYKDLAPQAD-GLDKAVAPEATT--AAPGATKGTY 187
           LGKGGAVLMVCR C KKGDHWTS+CPYKDLA   D  +DK    E++T  AAPG  K  Y
Sbjct: 130 LGKGGAVLMVCRICHKKGDHWTSKCPYKDLAAPTDVFIDKPPTGESSTMSAAPGTGKAAY 189

Query: 188 VPPGLRPGAERT--GTDMRRRNDENSVRVTNLSEDTREPDLLELFRPFGAVSRVYVAVDQ 247
           VPP +R GA+R+  G+DMRRRNDENSVRVTNLSEDTREPDL+ELF PFGAV+RVYVA+DQ
Sbjct: 190 VPPSMRAGADRSAVGSDMRRRNDENSVRVTNLSEDTREPDLMELFHPFGAVTRVYVAIDQ 249

Query: 248 KTSMSRGFGFVNFVNREDAQRSINKLNGYGYDNLILRVEWATPRAT 289
           KT +SRGFGFVNFV+REDAQR+INKLNGYGYDNLILRVEWATPR T
Sbjct: 250 KTGVSRGFGFVNFVSREDAQRAINKLNGYGYDNLILRVEWATPRPT 294

BLAST of MS019199 vs. TAIR 10
Match: AT3G11400.2 (eukaryotic translation initiation factor 3G1 )

HSP 1 Score: 414.5 bits (1064), Expect = 7.2e-116
Identity = 214/313 (68.37%), Postives = 245/313 (78.27%), Query Frame = 0

Query: 8   SKLRWGELDDDDGDLDFLLPPKKVIGPDENGLKKTVEYQFNEDGNKVKITTTSRTRKLAH 67
           SK RWGE+D+D+ DLDFLLPPK+VIGPDENGLK T+EY+FN++ NKVKITT +R RKLA 
Sbjct: 10  SKFRWGEMDEDE-DLDFLLPPKQVIGPDENGLKTTIEYKFNDEENKVKITTRTRVRKLAS 69

Query: 68  ARLSKHAVERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPG--------------- 127
           ARL+K A+ERR+W KFGDA +E+ GS LTMVSTEEILLERPRAPG               
Sbjct: 70  ARLNKRAMERRNWPKFGDAANEEAGSHLTMVSTEEILLERPRAPGDQLANHMLASIPEYE 129

Query: 128 ------------SKPEEPKVAGDPLAQLGKGGAVLMVCRTCGKKGDHWTSRCPYKDLAPQ 187
                       +K +E K  GD L+QLGKGGAVLMVCR C KKGDHWTS+CPYKDLA  
Sbjct: 130 LKCALTFVAANCTKADESKATGDGLSQLGKGGAVLMVCRICHKKGDHWTSKCPYKDLAAP 189

Query: 188 AD-GLDKAVAPEATT--AAPGATKGTYVPPGLRPGAERT--GTDMRRRNDENSVRVTNLS 247
            D  +DK    E++T  AAPG  K  YVPP +R GA+R+  G+DMRRRNDENSVRVTNLS
Sbjct: 190 TDVFIDKPPTGESSTMSAAPGTGKAAYVPPSMRAGADRSAVGSDMRRRNDENSVRVTNLS 249

Query: 248 EDTREPDLLELFRPFGAVSRVYVAVDQKTSMSRGFGFVNFVNREDAQRSINKLNGYGYDN 289
           EDTREPDL+ELF PFGAV+RVYVA+DQKT +SRGFGFVNFV+REDAQR+INKLNGYGYDN
Sbjct: 250 EDTREPDLMELFHPFGAVTRVYVAIDQKTGVSRGFGFVNFVSREDAQRAINKLNGYGYDN 309

BLAST of MS019199 vs. TAIR 10
Match: AT5G06000.1 (eukaryotic translation initiation factor 3G2 )

HSP 1 Score: 380.6 bits (976), Expect = 1.1e-105
Identity = 189/280 (67.50%), Postives = 227/280 (81.07%), Query Frame = 0

Query: 9   KLRWGELDDDDGDLDFLLPPKKVIGPDENGLKKTVEYQFNEDGNKVKITTTSRTRKLAHA 68
           KLRWGE+D+++GD DFLLPPK++I PD+NG+KK +EY+FNE+  KVKITTT+R +K A  
Sbjct: 11  KLRWGEIDEEEGDYDFLLPPKQMISPDQNGVKKVIEYKFNEEDKKVKITTTTRVQKRA-- 70

Query: 69  RLSKHAVERRSWAKFGDAVHEDVGSRLTMVSTEEILLERPRAPGSKPEEPKVAGDPLAQL 128
            L+K AVERRSW KFGDA HE+  S LTM STE+I+LER RAPGS  E+  V+GD ++QL
Sbjct: 71  -LTKQAVERRSWNKFGDAAHEESSSYLTMRSTEDIILERIRAPGSNAEQSTVSGDSMSQL 130

Query: 129 GKGGAVLMVCRTCGKKGDHWTSRCPYKDLAPQADGLDKAVAPEATTAAPGATKGTYVPPG 188
           GK GAVLMVCR C KKGDHWTSRCP KDL    D  +   A  +T+   G  +  YVPP 
Sbjct: 131 GKPGAVLMVCRLCQKKGDHWTSRCPQKDLLSLMD--EPLTAETSTSTITGTGRAAYVPPS 190

Query: 189 LRPGAERT--GTDMRRRNDENSVRVTNLSEDTREPDLLELFRPFGAVSRVYVAVDQKTSM 248
           +R GA+R   G+DMR R+DENSVRVTNLSEDTR PDL+ELFRPFGAV+R +VA+DQKTSM
Sbjct: 191 MREGADRKAGGSDMRSRHDENSVRVTNLSEDTRGPDLMELFRPFGAVTRCHVAIDQKTSM 250

Query: 249 SRGFGFVNFVNREDAQRSINKLNGYGYDNLILRVEWATPR 287
           SRGFGFV+FV+REDAQR+INKLNGYGYDNLILRVEW+TP+
Sbjct: 251 SRGFGFVSFVSREDAQRAINKLNGYGYDNLILRVEWSTPK 285

BLAST of MS019199 vs. TAIR 10
Match: AT1G54080.1 (oligouridylate-binding protein 1A )

HSP 1 Score: 67.4 bits (163), Expect = 2.1e-11
Identity = 37/90 (41.11%), Postives = 53/90 (58.89%), Query Frame = 0

Query: 202 RRNDENS---VRVTNLSEDTREPDLLELFRPFGAVSRVYVAVDQKTSMSRGFGFVNFVNR 261
           +R D +S   + V +LS +  +  L + F  F + S   V  DQKT  SRGFGFV+F N+
Sbjct: 140 QREDTSSHFNIFVGDLSPEVTDAALFDSFSAFNSCSDARVMWDQKTGRSRGFGFVSFRNQ 199

Query: 262 EDAQRSINKLNGYGYDNLILRVEWATPRAT 289
           +DAQ +IN++NG    +  +R  WAT  AT
Sbjct: 200 QDAQTAINEMNGKWVSSRQIRCNWATKGAT 229

BLAST of MS019199 vs. TAIR 10
Match: AT3G14100.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 67.0 bits (162), Expect = 2.8e-11
Identity = 37/90 (41.11%), Postives = 53/90 (58.89%), Query Frame = 0

Query: 202 RRNDENS---VRVTNLSEDTREPDLLELFRPFGAVSRVYVAVDQKTSMSRGFGFVNFVNR 261
           +R D +S   + V +LS +  +  L + F  F + S   V  DQKT  SRGFGFV+F N+
Sbjct: 136 QREDTSSHFNIFVGDLSPEVTDATLYQSFSVFSSCSDARVMWDQKTGRSRGFGFVSFRNQ 195

Query: 262 EDAQRSINKLNGYGYDNLILRVEWATPRAT 289
           +DAQ +IN++NG    +  +R  WAT  AT
Sbjct: 196 QDAQTAINEMNGKWLSSRQIRCNWATKGAT 225

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022143427.14.3e-16399.65eukaryotic translation initiation factor 3 subunit G-like [Momordica charantia][more]
XP_022949274.12.4e-15896.18eukaryotic translation initiation factor 3 subunit G-like [Cucurbita moschata] >... [more]
KAG6607041.19.3e-15895.83Eukaryotic translation initiation factor 3 subunit G, partial [Cucurbita argyros... [more]
XP_038876811.14.6e-15795.83eukaryotic translation initiation factor 3 subunit G-like [Benincasa hispida][more]
XP_022935522.11.3e-15695.49eukaryotic translation initiation factor 3 subunit G-like [Cucurbita moschata] >... [more]
Match NameE-valueIdentityDescription
Q6DRC43.3e-4939.93Eukaryotic translation initiation factor 3 subunit G OS=Danio rerio OX=7955 GN=e... [more]
A7SKE92.8e-4841.02Eukaryotic translation initiation factor 3 subunit G OS=Nematostella vectensis O... [more]
Q28CY22.8e-4840.65Eukaryotic translation initiation factor 3 subunit G OS=Xenopus tropicalis OX=83... [more]
Q6DJI81.4e-4743.68Eukaryotic translation initiation factor 3 subunit G-A OS=Xenopus laevis OX=8355... [more]
Q641B23.1e-4740.91Eukaryotic translation initiation factor 3 subunit G-B OS=Xenopus laevis OX=8355... [more]
Match NameE-valueIdentityDescription
A0A6J1CQS92.1e-16399.65Eukaryotic translation initiation factor 3 subunit G OS=Momordica charantia OX=3... [more]
A0A6J1KBW41.2e-15896.18Eukaryotic translation initiation factor 3 subunit G OS=Cucurbita maxima OX=3661... [more]
A0A6J1GCD41.2e-15896.18Eukaryotic translation initiation factor 3 subunit G OS=Cucurbita moschata OX=36... [more]
A0A6J1F5T26.5e-15795.49Eukaryotic translation initiation factor 3 subunit G OS=Cucurbita moschata OX=36... [more]
A0A6J1J7Q01.4e-15695.14Eukaryotic translation initiation factor 3 subunit G OS=Cucurbita maxima OX=3661... [more]
Match NameE-valueIdentityDescription
AT3G11400.12.8e-12074.83eukaryotic translation initiation factor 3G1 [more]
AT3G11400.27.2e-11668.37eukaryotic translation initiation factor 3G1 [more]
AT5G06000.11.1e-10567.50eukaryotic translation initiation factor 3G2 [more]
AT1G54080.12.1e-1141.11oligouridylate-binding protein 1A [more]
AT3G14100.12.8e-1141.11RNA-binding (RRM/RBD/RNP motifs) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000504RNA recognition motif domainSMARTSM00360rrm1_1coord: 208..281
e-value: 4.8E-21
score: 85.9
IPR000504RNA recognition motif domainPFAMPF00076RRM_1coord: 209..275
e-value: 3.6E-18
score: 65.2
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 207..285
score: 18.154507
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3D3.30.70.330coord: 177..288
e-value: 2.2E-24
score: 88.1
IPR017334Eukaryotic translation initiation factor 3 subunit GPIRSFPIRSF037949Transl_init_eIF-3_RNA-bindcoord: 1..288
e-value: 2.2E-86
score: 287.7
IPR017334Eukaryotic translation initiation factor 3 subunit GHAMAPMF_03006eIF3gcoord: 9..287
score: 27.831423
IPR024675Eukaryotic translation initiation factor 3 subunit G, N-terminalPFAMPF12353eIF3gcoord: 30..157
e-value: 1.4E-33
score: 115.9
IPR024675Eukaryotic translation initiation factor 3 subunit G, N-terminalCDDcd12933eIF3Gcoord: 37..156
e-value: 8.17018E-38
score: 127.786
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 176..206
NoneNo IPR availablePANTHERPTHR10352:SF34EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT Gcoord: 7..287
NoneNo IPR availablePANTHERPTHR10352EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT Gcoord: 7..287
IPR034240eIF3G, RNA recognition motifCDDcd12408RRM_eIF3G_likecoord: 208..283
e-value: 1.48713E-44
score: 143.834
IPR035979RNA-binding domain superfamilySUPERFAMILY54928RNA-binding domain, RBDcoord: 205..286

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS019199.1MS019199.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005852 eukaryotic translation initiation factor 3 complex
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003743 translation initiation factor activity