MS019181 (gene) Bitter gourd (TR) v1

Overview
NameMS019181
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionprotein TPX2
Locationscaffold20: 2332409 .. 2333066 (-)
RNA-Seq ExpressionMS019181
SyntenyMS019181
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCTTCTTAAGGTGAAAGATGGAGTTAGAGATCGGGTTTCGGAGAGAGCGGAGAGAGCTCGGGTGAGTTTTCGTGATTGGAATTTGAAGTTCTTCTTTGCCTTGTTTTGTGGAATGGAAGTGTGAGGTTCACTTTTGTTTCTTTGTTTTTTTAGGGTTCTCAAAAGGTTGTCGTGAAAGAAAACACCAAAACGCAAGATTTCAAATTGCATACGCAAGAAAGAGCGGTGAAACGGGCCATGTTCAACTACTCGGTGAGTTGAGTGCTGAGTTCTGATTACATATATTAGCTTAAGTTTTTAGGTTAAGTGGTGAGCTTTAATATGATCGTATCAATCGTTGTCTTGTCTATTAATATTTTTTATTCATCTTGTCGGGTCTTGTATTAATTTTCGAACCGACGTGCAGGTAGCAACCAAGTCATATGTGACGGAGATACAAAAGAAAATTGAAGAAAAGTTGCACAAGGTAAAAACTTGCACAATCCTTGCACAACACACACAAAGTTTTGCAATAAAACAGTGCAGAAAAAGAACTGAACAAAAACAAAATGTGAAAGCAGATGATTGAAGAGGAAGAGATTCGTTTGCTGAGAAAGGAGATGGTTCCAAGAGCTCAACTAATGCCCTACTTTGATAGACCCTACTTTCCACAAAGG

mRNA sequence

CTTCTTCTTAAGGTGAAAGATGGAGTTAGAGATCGGGTTTCGGAGAGAGCGGAGAGAGCTCGGGGTTCTCAAAAGGTTGTCGTGAAAGAAAACACCAAAACGCAAGATTTCAAATTGCATACGCAAGAAAGAGCGGTGAAACGGGCCATGTTCAACTACTCGGTAGCAACCAAGTCATATGTGACGGAGATACAAAAGAAAATTGAAGAAAAGTTGCACAAGATGATTGAAGAGGAAGAGATTCGTTTGCTGAGAAAGGAGATGGTTCCAAGAGCTCAACTAATGCCCTACTTTGATAGACCCTACTTTCCACAAAGG

Coding sequence (CDS)

CTTCTTCTTAAGGTGAAAGATGGAGTTAGAGATCGGGTTTCGGAGAGAGCGGAGAGAGCTCGGGGTTCTCAAAAGGTTGTCGTGAAAGAAAACACCAAAACGCAAGATTTCAAATTGCATACGCAAGAAAGAGCGGTGAAACGGGCCATGTTCAACTACTCGGTAGCAACCAAGTCATATGTGACGGAGATACAAAAGAAAATTGAAGAAAAGTTGCACAAGATGATTGAAGAGGAAGAGATTCGTTTGCTGAGAAAGGAGATGGTTCCAAGAGCTCAACTAATGCCCTACTTTGATAGACCCTACTTTCCACAAAGG

Protein sequence

LLLKVKDGVRDRVSERAERARGSQKVVVKENTKTQDFKLHTQERAVKRAMFNYSVATKSYVTEIQKKIEEKLHKMIEEEEIRLLRKEMVPRAQLMPYFDRPYFPQR
Homology
BLAST of MS019181 vs. NCBI nr
Match: XP_022143415.1 (protein TPX2 [Momordica charantia])

HSP 1 Score: 195.7 bits (496), Expect = 2.1e-46
Identity = 103/105 (98.10%), Postives = 104/105 (99.05%), Query Frame = 0

Query: 2   LLKVKDGVRDRVSERAERARGSQKVVVKENTKTQDFKLHTQERAVKRAMFNYSVATKSYV 61
           L+KVKDGVRDRVSERAERARGSQKVVVKENTK QDFKLHTQERAVKRAMFNYSVATKSYV
Sbjct: 9   LIKVKDGVRDRVSERAERARGSQKVVVKENTKMQDFKLHTQERAVKRAMFNYSVATKSYV 68

Query: 62  TEIQKKIEEKLHKMIEEEEIRLLRKEMVPRAQLMPYFDRPYFPQR 107
           TEIQKKIEEKLHKMIEEEEIRLLRKEMVPRAQLMPYFDRPYFPQR
Sbjct: 69  TEIQKKIEEKLHKMIEEEEIRLLRKEMVPRAQLMPYFDRPYFPQR 113

BLAST of MS019181 vs. NCBI nr
Match: XP_022949252.1 (protein TPX2-like [Cucurbita moschata])

HSP 1 Score: 173.3 bits (438), Expect = 1.1e-39
Identity = 89/105 (84.76%), Postives = 97/105 (92.38%), Query Frame = 0

Query: 3   LKVKDGVRDRVSERAERARGSQKVVVKENT-KTQDFKLHTQERAVKRAMFNYSVATKSYV 62
           +K KDGVRDRVSE+ ER R  QKVVVKENT K QDFKLHTQERAVKRAMFNYS+ATK YV
Sbjct: 10  IKAKDGVRDRVSEKTERVRTPQKVVVKENTKKPQDFKLHTQERAVKRAMFNYSIATKLYV 69

Query: 63  TEIQKKIEEKLHKMIEEEEIRLLRKEMVPRAQLMPYFDRPYFPQR 107
           TE+QKK+EEKLHKMIEEEE+RL+RKEM+PRAQLMPYFDRPYFPQR
Sbjct: 70  TELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQR 114

BLAST of MS019181 vs. NCBI nr
Match: KAG7036751.1 (Protein TPX2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 172.2 bits (435), Expect = 2.4e-39
Identity = 88/105 (83.81%), Postives = 97/105 (92.38%), Query Frame = 0

Query: 3   LKVKDGVRDRVSERAERARGSQKVVVKENT-KTQDFKLHTQERAVKRAMFNYSVATKSYV 62
           +K KDGVRDRVSE+ ER R  QKVVVK+NT K QDFKLHTQERAVKRAMFNYS+ATK YV
Sbjct: 10  IKAKDGVRDRVSEKTERVRTPQKVVVKDNTKKPQDFKLHTQERAVKRAMFNYSIATKLYV 69

Query: 63  TEIQKKIEEKLHKMIEEEEIRLLRKEMVPRAQLMPYFDRPYFPQR 107
           TE+QKK+EEKLHKMIEEEE+RL+RKEM+PRAQLMPYFDRPYFPQR
Sbjct: 70  TELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQR 114

BLAST of MS019181 vs. NCBI nr
Match: XP_004145652.1 (protein TPX2 [Cucumis sativus])

HSP 1 Score: 171.4 bits (433), Expect = 4.2e-39
Identity = 89/107 (83.18%), Postives = 98/107 (91.59%), Query Frame = 0

Query: 1   LLLKVKDGVRDRVSERAERARGSQKVVVKENT-KTQDFKLHTQERAVKRAMFNYSVATKS 60
           L+ K KDGVRDRV E+AERAR  QK  VKENT K+QDFKLHTQERAVKRAMFNYS+ATK 
Sbjct: 9   LMTKTKDGVRDRVLEKAERARVPQKAHVKENTKKSQDFKLHTQERAVKRAMFNYSIATKL 68

Query: 61  YVTEIQKKIEEKLHKMIEEEEIRLLRKEMVPRAQLMPYFDRPYFPQR 107
           YVTE+QKK+EEKLHKMIEEEE+RL+RKEM+PRAQLMPYFDRPYFPQR
Sbjct: 69  YVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQR 115

BLAST of MS019181 vs. NCBI nr
Match: XP_023525926.1 (protein TPX2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 171.0 bits (432), Expect = 5.5e-39
Identity = 87/105 (82.86%), Postives = 97/105 (92.38%), Query Frame = 0

Query: 3   LKVKDGVRDRVSERAERARGSQKVVVKENT-KTQDFKLHTQERAVKRAMFNYSVATKSYV 62
           +K KDGVRDRVS++ ER R  QKVVVK+NT K QDFKLHTQERAVKRAMFNYS+ATK YV
Sbjct: 10  IKAKDGVRDRVSDKTERVRTPQKVVVKDNTKKPQDFKLHTQERAVKRAMFNYSIATKLYV 69

Query: 63  TEIQKKIEEKLHKMIEEEEIRLLRKEMVPRAQLMPYFDRPYFPQR 107
           TE+QKK+EEKLHKMIEEEE+RL+RKEM+PRAQLMPYFDRPYFPQR
Sbjct: 70  TELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQR 114

BLAST of MS019181 vs. ExPASy Swiss-Prot
Match: F4I2H7 (Protein TPX2 OS=Arabidopsis thaliana OX=3702 GN=TPX2 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 3.1e-05
Identity = 34/106 (32.08%), Postives = 61/106 (57.55%), Query Frame = 0

Query: 14  SERAERARGSQKVVVKEN------------TKTQDFKLHTQERAVKRAMFNYSVATKSYV 73
           +E A++     + V+KE+            T+ Q+F LH + RAV+RA F++ +  K   
Sbjct: 612 TEEAQKRLFKAQPVIKEDPIPVPEKVRMPLTEIQEFNLHVEHRAVERADFDHKIKEKENQ 671

Query: 74  TEIQKKIEEKLHKMIEEEE-IRLLRKEMVPRAQLMPYFDRPYFPQR 107
            +  ++ E +  KM+EEE  ++ +RK MVP A+ +P F++P+ PQ+
Sbjct: 672 YKRYRE-ESEAAKMVEEERALKQMRKTMVPHARPVPNFNKPFLPQK 716

BLAST of MS019181 vs. ExPASy TrEMBL
Match: A0A6J1CNR5 (protein TPX2 OS=Momordica charantia OX=3673 GN=LOC111013295 PE=3 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 1.0e-46
Identity = 103/105 (98.10%), Postives = 104/105 (99.05%), Query Frame = 0

Query: 2   LLKVKDGVRDRVSERAERARGSQKVVVKENTKTQDFKLHTQERAVKRAMFNYSVATKSYV 61
           L+KVKDGVRDRVSERAERARGSQKVVVKENTK QDFKLHTQERAVKRAMFNYSVATKSYV
Sbjct: 9   LIKVKDGVRDRVSERAERARGSQKVVVKENTKMQDFKLHTQERAVKRAMFNYSVATKSYV 68

Query: 62  TEIQKKIEEKLHKMIEEEEIRLLRKEMVPRAQLMPYFDRPYFPQR 107
           TEIQKKIEEKLHKMIEEEEIRLLRKEMVPRAQLMPYFDRPYFPQR
Sbjct: 69  TEIQKKIEEKLHKMIEEEEIRLLRKEMVPRAQLMPYFDRPYFPQR 113

BLAST of MS019181 vs. ExPASy TrEMBL
Match: A0A6J1GBJ5 (protein TPX2-like OS=Cucurbita moschata OX=3662 GN=LOC111452663 PE=3 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 5.3e-40
Identity = 89/105 (84.76%), Postives = 97/105 (92.38%), Query Frame = 0

Query: 3   LKVKDGVRDRVSERAERARGSQKVVVKENT-KTQDFKLHTQERAVKRAMFNYSVATKSYV 62
           +K KDGVRDRVSE+ ER R  QKVVVKENT K QDFKLHTQERAVKRAMFNYS+ATK YV
Sbjct: 10  IKAKDGVRDRVSEKTERVRTPQKVVVKENTKKPQDFKLHTQERAVKRAMFNYSIATKLYV 69

Query: 63  TEIQKKIEEKLHKMIEEEEIRLLRKEMVPRAQLMPYFDRPYFPQR 107
           TE+QKK+EEKLHKMIEEEE+RL+RKEM+PRAQLMPYFDRPYFPQR
Sbjct: 70  TELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQR 114

BLAST of MS019181 vs. ExPASy TrEMBL
Match: A0A0A0LCD4 (TPX2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G258180 PE=3 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 2.0e-39
Identity = 89/107 (83.18%), Postives = 98/107 (91.59%), Query Frame = 0

Query: 1   LLLKVKDGVRDRVSERAERARGSQKVVVKENT-KTQDFKLHTQERAVKRAMFNYSVATKS 60
           L+ K KDGVRDRV E+AERAR  QK  VKENT K+QDFKLHTQERAVKRAMFNYS+ATK 
Sbjct: 9   LMTKTKDGVRDRVLEKAERARVPQKAHVKENTKKSQDFKLHTQERAVKRAMFNYSIATKL 68

Query: 61  YVTEIQKKIEEKLHKMIEEEEIRLLRKEMVPRAQLMPYFDRPYFPQR 107
           YVTE+QKK+EEKLHKMIEEEE+RL+RKEM+PRAQLMPYFDRPYFPQR
Sbjct: 69  YVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQR 115

BLAST of MS019181 vs. ExPASy TrEMBL
Match: A0A6J1K9I8 (protein TPX2 OS=Cucurbita maxima OX=3661 GN=LOC111492897 PE=3 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 7.7e-39
Identity = 86/105 (81.90%), Postives = 96/105 (91.43%), Query Frame = 0

Query: 3   LKVKDGVRDRVSERAERARGSQKVVVKENT-KTQDFKLHTQERAVKRAMFNYSVATKSYV 62
           +K KDG+RDRVS++ ER R  QKVVVKENT K QDFKLHTQERAVKRAMFNYS+ATK YV
Sbjct: 10  IKAKDGIRDRVSDKTERVRTPQKVVVKENTKKPQDFKLHTQERAVKRAMFNYSIATKLYV 69

Query: 63  TEIQKKIEEKLHKMIEEEEIRLLRKEMVPRAQLMPYFDRPYFPQR 107
            E+QKK+EEKLHKMIEEEE+RL+RKEM+PRAQLMPYFDRPYFPQR
Sbjct: 70  MELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQR 114

BLAST of MS019181 vs. ExPASy TrEMBL
Match: A0A5D3DX27 (Protein TPX2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold600G00390 PE=3 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 7.7e-39
Identity = 88/107 (82.24%), Postives = 96/107 (89.72%), Query Frame = 0

Query: 1   LLLKVKDGVRDRVSERAERARGSQKVVVKENT-KTQDFKLHTQERAVKRAMFNYSVATKS 60
           L+ K KDGVRDRV E+ ERAR  QK  VKENT K QDFKLHTQERAVKRAMFNYS+ATK 
Sbjct: 9   LMTKTKDGVRDRVLEKVERARVPQKAPVKENTKKPQDFKLHTQERAVKRAMFNYSIATKL 68

Query: 61  YVTEIQKKIEEKLHKMIEEEEIRLLRKEMVPRAQLMPYFDRPYFPQR 107
           YVTE+QKK+EEKLHKMIEEEE+RL+RKEM+PRAQLMPYFDRPYFPQR
Sbjct: 69  YVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQR 115

BLAST of MS019181 vs. TAIR 10
Match: AT5G37478.1 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 115.9 bits (289), Expect = 1.9e-26
Identity = 62/102 (60.78%), Postives = 77/102 (75.49%), Query Frame = 0

Query: 5   VKDGVRDRVSERAERARGSQKVVVKENTKTQDFKLHTQERAVKRAMFNYSVATKSYVTEI 64
           +K+   D+   +A   +   K   KEN K  +FKLH+ ERAVKRAMFNYSVAT  Y+ ++
Sbjct: 40  LKERAHDKT--KAATTKNITKAPAKENKKPLEFKLHSGERAVKRAMFNYSVATNYYIQKL 99

Query: 65  QKKIEEKLHKMIEEEEIRLLRKEMVPRAQLMPYFDRPYFPQR 107
           QKK EE+L KMIEEEEIR+LRKEMVP+AQLMP+FDRP+ PQR
Sbjct: 100 QKKQEERLQKMIEEEEIRMLRKEMVPKAQLMPFFDRPFLPQR 139

BLAST of MS019181 vs. TAIR 10
Match: AT3G01015.1 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 76.6 bits (187), Expect = 1.3e-14
Identity = 43/79 (54.43%), Postives = 54/79 (68.35%), Query Frame = 0

Query: 28  VKENTKTQDFKLHTQERAVKRAMFNYSVATKSYVTEIQKKIEEKLHKMIEEEEIRLLRKE 87
           VK+ T   D KLH+  RAV+RA F+Y V  K  + E  K   E+  K+ EEEEIR LRKE
Sbjct: 365 VKDITIPVDLKLHSDIRAVERAEFDYQVTEKINLVEQYKTERERQQKLAEEEEIRRLRKE 424

Query: 88  MVPRAQLMPYFDRPYFPQR 107
           +VP+AQ MPYFDRP+ P+R
Sbjct: 425 LVPKAQPMPYFDRPFIPKR 443

BLAST of MS019181 vs. TAIR 10
Match: AT5G15510.1 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 75.9 bits (185), Expect = 2.2e-14
Identity = 42/78 (53.85%), Postives = 53/78 (67.95%), Query Frame = 0

Query: 29  KENTKTQDFKLHTQERAVKRAMFNYSVATKSYVTEIQKKIEEKLHKMIEEEEIRLLRKEM 88
           K+ T+  D  LH+  RAV+RA F+Y VA K    E  K   E+  K+ EEEEIR LRKE+
Sbjct: 376 KDITRPVDLTLHSDVRAVERAEFDYQVAEKMSFIEQYKMERERQQKLAEEEEIRRLRKEL 435

Query: 89  VPRAQLMPYFDRPYFPQR 107
           VP+AQ MPYFDRP+ P+R
Sbjct: 436 VPKAQPMPYFDRPFIPRR 453

BLAST of MS019181 vs. TAIR 10
Match: AT5G15510.2 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 75.9 bits (185), Expect = 2.2e-14
Identity = 42/78 (53.85%), Postives = 53/78 (67.95%), Query Frame = 0

Query: 29  KENTKTQDFKLHTQERAVKRAMFNYSVATKSYVTEIQKKIEEKLHKMIEEEEIRLLRKEM 88
           K+ T+  D  LH+  RAV+RA F+Y VA K    E  K   E+  K+ EEEEIR LRKE+
Sbjct: 376 KDITRPVDLTLHSDVRAVERAEFDYQVAEKMSFIEQYKMERERQQKLAEEEEIRRLRKEL 435

Query: 89  VPRAQLMPYFDRPYFPQR 107
           VP+AQ MPYFDRP+ P+R
Sbjct: 436 VPKAQPMPYFDRPFIPRR 453

BLAST of MS019181 vs. TAIR 10
Match: AT1G03780.2 (targeting protein for XKLP2 )

HSP 1 Score: 49.3 bits (116), Expect = 2.2e-06
Identity = 34/106 (32.08%), Postives = 61/106 (57.55%), Query Frame = 0

Query: 14  SERAERARGSQKVVVKEN------------TKTQDFKLHTQERAVKRAMFNYSVATKSYV 73
           +E A++     + V+KE+            T+ Q+F LH + RAV+RA F++ +  K   
Sbjct: 612 TEEAQKRLFKAQPVIKEDPIPVPEKVRMPLTEIQEFNLHVEHRAVERADFDHKIKEKENQ 671

Query: 74  TEIQKKIEEKLHKMIEEEE-IRLLRKEMVPRAQLMPYFDRPYFPQR 107
            +  ++ E +  KM+EEE  ++ +RK MVP A+ +P F++P+ PQ+
Sbjct: 672 YKRYRE-ESEAAKMVEEERALKQMRKTMVPHARPVPNFNKPFLPQK 716

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022143415.12.1e-4698.10protein TPX2 [Momordica charantia][more]
XP_022949252.11.1e-3984.76protein TPX2-like [Cucurbita moschata][more]
KAG7036751.12.4e-3983.81Protein TPX2 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_004145652.14.2e-3983.18protein TPX2 [Cucumis sativus][more]
XP_023525926.15.5e-3982.86protein TPX2-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
F4I2H73.1e-0532.08Protein TPX2 OS=Arabidopsis thaliana OX=3702 GN=TPX2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CNR51.0e-4698.10protein TPX2 OS=Momordica charantia OX=3673 GN=LOC111013295 PE=3 SV=1[more]
A0A6J1GBJ55.3e-4084.76protein TPX2-like OS=Cucurbita moschata OX=3662 GN=LOC111452663 PE=3 SV=1[more]
A0A0A0LCD42.0e-3983.18TPX2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G258180 PE=3 S... [more]
A0A6J1K9I87.7e-3981.90protein TPX2 OS=Cucurbita maxima OX=3661 GN=LOC111492897 PE=3 SV=1[more]
A0A5D3DX277.7e-3982.24Protein TPX2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold600G00390 P... [more]
Match NameE-valueIdentityDescription
AT5G37478.11.9e-2660.78TPX2 (targeting protein for Xklp2) protein family [more]
AT3G01015.11.3e-1454.43TPX2 (targeting protein for Xklp2) protein family [more]
AT5G15510.12.2e-1453.85TPX2 (targeting protein for Xklp2) protein family [more]
AT5G15510.22.2e-1453.85TPX2 (targeting protein for Xklp2) protein family [more]
AT1G03780.22.2e-0632.08targeting protein for XKLP2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027329TPX2, C-terminalPFAMPF06886TPX2coord: 37..105
e-value: 1.8E-18
score: 66.5
NoneNo IPR availablePANTHERPTHR14326:SF39TPX2 (TARGETING PROTEIN FOR XKLP2) PROTEIN FAMILYcoord: 8..106
IPR009675TPX2PANTHERPTHR14326TARGETING PROTEIN FOR XKLP2coord: 8..106

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS019181.1MS019181.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032147 activation of protein kinase activity
biological_process GO:0060236 regulation of mitotic spindle organization
cellular_component GO:0005874 microtubule
cellular_component GO:0005819 spindle