MS019170 (gene) Bitter gourd (TR) v1

BLAST of MS019170 vs. NCBI nr
Match: XP_022143323.1 (valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Momordica charantia])

HSP 1 Score: 1574.7 bits (4076), Expect = 0.0e+00
Identity = 811/966 (83.95%), Postives = 813/966 (84.16%), Query Frame = 0

Query: 1   MLLQMPFSGPSLLPPYRLNPLLFTRHRPAFRLSRSHFRRLRPTQFTVAASANGVFTSPEV 60
           MLLQMPFSGPSLLPPYRLNPLLFTRHRPAFRLSRSHF RLRPTQFTVAASANGVFTSPEV
Sbjct: 1   MLLQMPFSGPSLLPPYRLNPLLFTRHRPAFRLSRSHFCRLRPTQFTVAASANGVFTSPEV 60

Query: 61  AKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE--- 120
           AKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE   
Sbjct: 61  AKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLEDIM 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 VRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEKYGG 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 TITNQIKRLGASCDWTREHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPSLQTAV 240

Query: 241 ---EVEYSEESGTLYYIKYRVAGGSKSDYLTIATTRPETLFGDVAIAVNPQDDRYSKYVG 300
              EVEYSEESGTLYYIKYRVAGGSKSDYLTIATTRPETLFGDVAIAVNPQDDRYSKYVG
Sbjct: 241 SDLEVEYSEESGTLYYIKYRVAGGSKSDYLTIATTRPETLFGDVAIAVNPQDDRYSKYVG 300

Query: 301 MMAIVPMTYGRHVPIISDKYVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNKDGT 360
           MMAIVPMTYGRHVPIISDK+VDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNKDGT
Sbjct: 301 MMAIVPMTYGRHVPIISDKHVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNKDGT 360

Query: 361 LNKVAGLYRGLDRFEARKKLWADLEETGLAVRKEAHTLRVPRSQRGGEIIEPLVSKQWFM 420
           LNKVAGLYRGLDRFEARKKLWADLEETGLAVRKEAHTLRVPRSQRGGEIIEPLVSKQWFM
Sbjct: 361 LNKVAGLYRGLDRFEARKKLWADLEETGLAVRKEAHTLRVPRSQRGGEIIEPLVSKQWFM 420

Query: 421 TMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYVVGKN 480
           TMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYVVGKN
Sbjct: 421 TMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYVVGKN 480

Query: 481 PEEEYIVARNAGEALEQAHKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAEDFK 540
           PEEEYIVARNAGEALEQAHKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAEDFK
Sbjct: 481 PEEEYIVARNAGEALEQAHKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAEDFK 540

Query: 541 RFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLGNVI 600
           RFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLGNVI
Sbjct: 541 RFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLGNVI 600

Query: 601 DPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPTQND 660
           DPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPTQND
Sbjct: 601 DPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPTQND 660

Query: 661 SRSWESILSFEFEKEDCLLKLSLPECWIVSELHSLIDMVTTSYDKFFFGDIGRDVYNFFW 720
           SRSWESILSFEFEKEDCLLKLSLPECWIVSELHSLIDMVTTSYDKFFFGDIGRDVYNFFW
Sbjct: 661 SRSWESILSFEFEKEDCLLKLSLPECWIVSELHSLIDMVTTSYDKFFFGDIGRDVYNFFW 720

Query: 721 GDFADWYIEASKARLYQSGGHSVTLAQAVLLYVFENILKLLHPFMPFVTEELWQALPHRK 780
           GDFADWYIEASKARLYQSGGHSVTLAQAVLLYVFENILKLLHPFMPFVTEELWQALPHRK
Sbjct: 721 GDFADWYIEASKARLYQSGGHSVTLAQAVLLYVFENILKLLHPFMPFVTEELWQALPHRK 780

Query: 781 EALIISPWPQSSLPRQASAVKKFENLKLLTRAIRNARAEYSVESAKRISASIVASEEVNQ 818
           EALIISPWPQSSLPRQASAVKKFENLKLLTRAIRNARAEYSVESAKRISASIVASEEVNQ
Sbjct: 781 EALIISPWPQSSLPRQASAVKKFENLKLLTRAIRNARAEYSVESAKRISASIVASEEVNQ 840

BLAST of MS019170 vs. NCBI nr
Match: KAG6581186.1 (Valine--tRNA ligase, chloroplastic/mitochondrial 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1484.5 bits (3842), Expect = 0.0e+00
Identity = 767/968 (79.24%), Postives = 787/968 (81.30%), Query Frame = 0

Query: 1   MLLQMPFSGPSLLPP---YRLNPLLFTRHRPAFRLSRSHFRRLRPTQFTVAASANGVFTS 60
           MLLQM FSGPSLL P   YRLNPL+FT+ R +F LSR HFRRL+  Q  VAASANGVFTS
Sbjct: 1   MLLQMAFSGPSLLHPSSAYRLNPLIFTKRRRSFGLSRFHFRRLQHRQLAVAASANGVFTS 60

Query: 61  PEVAKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 120
           PE+AKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE
Sbjct: 61  PEIAKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPHLQ 240

Query: 241 ------EVEYSEESGTLYYIKYRVAGGSKSDYLTIATTRPETLFGDVAIAVNPQDDRYSK 300
                 EVEYSEESGTLY+IKYRVAGGS+SDYLT+ATTRPETLFGDVAIAV+PQDDRYSK
Sbjct: 241 TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQDDRYSK 300

Query: 301 YVGMMAIVPMTYGRHVPIISDKYVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 360
           YVGMMAIVPMTYGRHVPIISDK VDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK
Sbjct: 301 YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 360

Query: 361 DGTLNKVAGLYRGLDRFEARKKLWADLEETGLAVRKEAHTLRVPRSQRGGEIIEPLVSKQ 420
           DGTLNKVAGLY GLDRFEARKKLWADLEETGLAV+KEAHTLRVPRSQRGGEIIEPLVSKQ
Sbjct: 361 DGTLNKVAGLYCGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ 420

Query: 421 WFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYVV 480
           WFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWY+V
Sbjct: 421 WFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV 480

Query: 481 GKNPEEEYIVARNAGEALEQAHKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 540
           GKNPEE+YIVARNA EA EQAHKKYGK VEIYQDPDVLDTWFSSALWPFSTLGWPDETAE
Sbjct: 481 GKNPEEDYIVARNADEAHEQAHKKYGKGVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 540

Query: 541 DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 600
           DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG
Sbjct: 541 DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 600

Query: 601 NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT 660
           NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT
Sbjct: 601 NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT 660

Query: 661 QNDSRSWESILSFEFEKEDCLLKLSLPECWIVSELHSLIDMVTTSYDKFFFGDIGRDVYN 720
           QNDS+SW+SILS+EFEKEDCLLKL LPECWIVSELHSLID+VT SYDKFFFGD+GRD+YN
Sbjct: 661 QNDSQSWKSILSYEFEKEDCLLKLPLPECWIVSELHSLIDVVTASYDKFFFGDVGRDIYN 720

Query: 721 FFWGDFADWYIEASKARLYQSGGHSVTLAQAVLLYVFENILKLLHPFMPFVTEELWQALP 780
           FFWGDFADWYIEASKARLYQSGG SV LAQAVLLYVFENILKLLHPFMPFVTEELWQALP
Sbjct: 721 FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP 780

Query: 781 HRKEALIISPWPQSSLPRQASAVKKFENLKLLTRAIRNARAEYSVESAKRISASIVASEE 817
           HRKEALIISPWPQ SLPRQA AVKKFENLKLLT+AIRNARAEYSVE AKRISASIVASEE
Sbjct: 781 HRKEALIISPWPQISLPRQAGAVKKFENLKLLTKAIRNARAEYSVEPAKRISASIVASEE 840

BLAST of MS019170 vs. NCBI nr
Match: XP_023528105.1 (LOW QUALITY PROTEIN: valine--tRNA ligase, chloroplastic/mitochondrial 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1481.8 bits (3835), Expect = 0.0e+00
Identity = 765/968 (79.03%), Postives = 786/968 (81.20%), Query Frame = 0

Query: 1   MLLQMPFSGPSLLPP---YRLNPLLFTRHRPAFRLSRSHFRRLRPTQFTVAASANGVFTS 60
           MLLQM FSGPSLL P   YRLNPL+FT+ R +F LSR HFRRL+  Q  VAASANGVFTS
Sbjct: 1   MLLQMAFSGPSLLHPSSAYRLNPLIFTKRRRSFGLSRFHFRRLQQRQLAVAASANGVFTS 60

Query: 61  PEVAKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 120
           PE+AKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE
Sbjct: 61  PEIAKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPHLQ 240

Query: 241 ------EVEYSEESGTLYYIKYRVAGGSKSDYLTIATTRPETLFGDVAIAVNPQDDRYSK 300
                 EVEYSEESGTLY+IKYRVAGGS+SDYLT+ATTRPETLFGDVAIAV+PQDDRYSK
Sbjct: 241 TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQDDRYSK 300

Query: 301 YVGMMAIVPMTYGRHVPIISDKYVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 360
           YVGMMAIVPMTYGRHVPIISDK VDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK
Sbjct: 301 YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 360

Query: 361 DGTLNKVAGLYRGLDRFEARKKLWADLEETGLAVRKEAHTLRVPRSQRGGEIIEPLVSKQ 420
           DGTLNKVAGLY GLDRFEARKKLWADLEETGLAV+KEAHTLRVPRSQRGGEIIEPLVSKQ
Sbjct: 361 DGTLNKVAGLYCGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ 420

Query: 421 WFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYVV 480
           WFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWY+V
Sbjct: 421 WFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV 480

Query: 481 GKNPEEEYIVARNAGEALEQAHKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 540
           GKNPEE+YIVARNA EA EQAHKKYGK VEIYQDPDVLDTWFSSALWPFSTLGWPDETAE
Sbjct: 481 GKNPEEDYIVARNADEAHEQAHKKYGKGVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 540

Query: 541 DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 600
           DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG
Sbjct: 541 DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 600

Query: 601 NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT 660
           NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT
Sbjct: 601 NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT 660

Query: 661 QNDSRSWESILSFEFEKEDCLLKLSLPECWIVSELHSLIDMVTTSYDKFFFGDIGRDVYN 720
           QNDS+SW+SILS+EFEKEDCLLKL LPECWIVSELHSLID+VT SYDKFFFGD+GRD+YN
Sbjct: 661 QNDSQSWKSILSYEFEKEDCLLKLPLPECWIVSELHSLIDVVTASYDKFFFGDVGRDIYN 720

Query: 721 FFWGDFADWYIEASKARLYQSGGHSVTLAQAVLLYVFENILKLLHPFMPFVTEELWQALP 780
           FFWGDFADWYIEASKARLYQSGG SV LAQAVLLYVFENILKLLHPFMPFVTEELWQALP
Sbjct: 721 FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP 780

Query: 781 HRKEALIISPWPQSSLPRQASAVKKFENLKLLTRAIRNARAEYSVESAKRISASIVASEE 817
           HRKEALIISPWPQ  LPRQA AVKKFENLKLLT+AIRNARAEYSVE AKRISASIVASEE
Sbjct: 781 HRKEALIISPWPQILLPRQAGAVKKFENLKLLTKAIRNARAEYSVEPAKRISASIVASEE 840

BLAST of MS019170 vs. NCBI nr
Match: XP_022983417.1 (valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1481.5 bits (3834), Expect = 0.0e+00
Identity = 765/968 (79.03%), Postives = 787/968 (81.30%), Query Frame = 0

Query: 1   MLLQMPFSGPSLLPP---YRLNPLLFTRHRPAFRLSRSHFRRLRPTQFTVAASANGVFTS 60
           MLLQM FSGPSLL P   YRLNPL+FT+ R +F LSR HFRRL+  Q  VAASANGVFTS
Sbjct: 1   MLLQMAFSGPSLLHPSSAYRLNPLIFTKCRRSFGLSRFHFRRLQQRQLAVAASANGVFTS 60

Query: 61  PEVAKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 120
           PE+AKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE
Sbjct: 61  PEIAKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPHLQ 240

Query: 241 ------EVEYSEESGTLYYIKYRVAGGSKSDYLTIATTRPETLFGDVAIAVNPQDDRYSK 300
                 EVEYSEESGTLY+IKYRVAGGS+SDYLT+ATTRPETLFGDVAIAV+PQDDRYSK
Sbjct: 241 TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQDDRYSK 300

Query: 301 YVGMMAIVPMTYGRHVPIISDKYVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 360
           YVGMMAIVPMTYGRHVPIISDK VDKDFGTGVLKISPGHDHNDYLLARK GLPILNVMNK
Sbjct: 301 YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKFGLPILNVMNK 360

Query: 361 DGTLNKVAGLYRGLDRFEARKKLWADLEETGLAVRKEAHTLRVPRSQRGGEIIEPLVSKQ 420
           DGTLNKVAGLY GLDRFEARKKLWADLEETGLAV+KEAHTLRVPRSQRGGEIIEPLVSKQ
Sbjct: 361 DGTLNKVAGLYCGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ 420

Query: 421 WFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYVV 480
           WFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWY+V
Sbjct: 421 WFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV 480

Query: 481 GKNPEEEYIVARNAGEALEQAHKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 540
           GKNPEE+YIVARNA EA EQAHKKYGK VEIYQDPDVLDTWFSSALWPFSTLGWPDETAE
Sbjct: 481 GKNPEEDYIVARNADEAHEQAHKKYGKGVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 540

Query: 541 DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 600
           DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG
Sbjct: 541 DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 600

Query: 601 NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT 660
           NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT
Sbjct: 601 NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT 660

Query: 661 QNDSRSWESILSFEFEKEDCLLKLSLPECWIVSELHSLIDMVTTSYDKFFFGDIGRDVYN 720
           QNDS+SW+SILS+EFEKEDCLLKL LPECWIVSELHSLID+VT SYDKFFFGD+GRD+YN
Sbjct: 661 QNDSQSWKSILSYEFEKEDCLLKLPLPECWIVSELHSLIDVVTASYDKFFFGDVGRDIYN 720

Query: 721 FFWGDFADWYIEASKARLYQSGGHSVTLAQAVLLYVFENILKLLHPFMPFVTEELWQALP 780
           FFWGDFADWYIEASKARLYQSGG+SV LAQAVLLYVFENILKLLHPFMPFVTEELWQALP
Sbjct: 721 FFWGDFADWYIEASKARLYQSGGNSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP 780

Query: 781 HRKEALIISPWPQSSLPRQASAVKKFENLKLLTRAIRNARAEYSVESAKRISASIVASEE 817
           HRKEALIISPWPQ  LPRQA AVKKFENLKLLT+AIRNARAEYSVE AKRISASIVASEE
Sbjct: 781 HRKEALIISPWPQILLPRQAGAVKKFENLKLLTKAIRNARAEYSVEPAKRISASIVASEE 840

BLAST of MS019170 vs. NCBI nr
Match: XP_022935322.1 (valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1478.0 bits (3825), Expect = 0.0e+00
Identity = 764/968 (78.93%), Postives = 784/968 (80.99%), Query Frame = 0

Query: 1   MLLQMPFSGPSLLPP---YRLNPLLFTRHRPAFRLSRSHFRRLRPTQFTVAASANGVFTS 60
           MLLQM FSGPSLL P   YRLNPL+FT+ R +F LSR HFRRL   Q  VAASANGVFTS
Sbjct: 1   MLLQMAFSGPSLLHPSSAYRLNPLIFTKRRRSFGLSRFHFRRLLHRQLAVAASANGVFTS 60

Query: 61  PEVAKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 120
           PE+AKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE
Sbjct: 61  PEIAKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPHLQ 240

Query: 241 ------EVEYSEESGTLYYIKYRVAGGSKSDYLTIATTRPETLFGDVAIAVNPQDDRYSK 300
                 EVEYSEESGTLY+IKYRVAGGS+SDYLT+ATTRPETLFGDVAIAV+PQDDRYSK
Sbjct: 241 TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQDDRYSK 300

Query: 301 YVGMMAIVPMTYGRHVPIISDKYVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 360
           YVGMMAIVPMTYGRHVPIISDK VDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK
Sbjct: 301 YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 360

Query: 361 DGTLNKVAGLYRGLDRFEARKKLWADLEETGLAVRKEAHTLRVPRSQRGGEIIEPLVSKQ 420
           DGTLNKVAGLY GLDRFEARKKLW DLEETGLAV+KEAHTLRVPRSQRGGEIIEPLVSKQ
Sbjct: 361 DGTLNKVAGLYCGLDRFEARKKLWVDLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ 420

Query: 421 WFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYVV 480
           WFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWY+V
Sbjct: 421 WFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV 480

Query: 481 GKNPEEEYIVARNAGEALEQAHKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 540
           GKNPEE+YIVARNA EA EQAHKKYGK VEIYQDPDVLDTWFSSALWPFSTLGWPDETAE
Sbjct: 481 GKNPEEDYIVARNADEAHEQAHKKYGKGVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 540

Query: 541 DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 600
           DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG
Sbjct: 541 DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 600

Query: 601 NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT 660
           NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT
Sbjct: 601 NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT 660

Query: 661 QNDSRSWESILSFEFEKEDCLLKLSLPECWIVSELHSLIDMVTTSYDKFFFGDIGRDVYN 720
           QNDS+SW+SILS+EFEKEDCLLKL LPECWIVSELHS ID+VT SYDKFFFGD+GRD+YN
Sbjct: 661 QNDSQSWKSILSYEFEKEDCLLKLPLPECWIVSELHSQIDVVTASYDKFFFGDVGRDIYN 720

Query: 721 FFWGDFADWYIEASKARLYQSGGHSVTLAQAVLLYVFENILKLLHPFMPFVTEELWQALP 780
           FFWGDFADWYIEASKARLYQSGG SV LAQAVLLYVFENILKLLHPFMPFVTEELWQALP
Sbjct: 721 FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP 780

Query: 781 HRKEALIISPWPQSSLPRQASAVKKFENLKLLTRAIRNARAEYSVESAKRISASIVASEE 817
           HRKEALIISPWPQ  LPRQA AVKKFENLKLLT+AIRNARAEYSVE AKRISASIVASEE
Sbjct: 781 HRKEALIISPWPQILLPRQAGAVKKFENLKLLTKAIRNARAEYSVEPAKRISASIVASEE 840

BLAST of MS019170 vs. ExPASy Swiss-Prot
Match: F4KE63 (Valine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana OX=3702 GN=EMB2247 PE=3 SV=1)

HSP 1 Score: 1208.0 bits (3124), Expect = 0.0e+00
Identity = 630/971 (64.88%), Postives = 715/971 (73.64%), Query Frame = 0

Query: 1   MLLQMPFSGPS-----LLP--PYRLNPLLFTRHRPAFRLSRSHFRRL---RPTQFTVAAS 60
           M+L+  FS P+     L P  P++LN L FTR R    +S S    +   R   F+ AAS
Sbjct: 1   MILKTAFSLPTPTTTLLSPSSPHQLNTLFFTRRRRRL-ISPSRLNSIFSQRRFSFSAAAS 60

Query: 61  ANGVFTSPEVAKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGH 120
            N VFTSPE +KTFDF+SEE+IY+WWESQGYF+P+FDQ   PFVI MPPPNVTGSLHMGH
Sbjct: 61  GNNVFTSPETSKTFDFSSEEKIYKWWESQGYFKPNFDQGGSPFVIPMPPPNVTGSLHMGH 120

Query: 121 AMFVTLE----------------------------------------------------- 180
           AMFVTLE                                                     
Sbjct: 121 AMFVTLEDIMVRYNRMNGRPTLWLPGTDHAGIATQLVVEKMLASEGIKRVDLGRDEFTKR 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 VWEWKEKYGGTITNQIKRLGASCDWSRERFTLDEQLSRAVVEAFVKLHDKGLIYQGSYMV 240

Query: 241 -------------EVEYSEESGTLYYIKYRVAGGSKSDYLTIATTRPETLFGDVAIAVNP 300
                        EVEYSEE G LY+IKYRVAG    D+LTIATTRPETLFGDVA+AV+P
Sbjct: 241 NWSPNLQTAVSDLEVEYSEEPGFLYHIKYRVAG--SPDFLTIATTRPETLFGDVALAVHP 300

Query: 301 QDDRYSKYVGMMAIVPMTYGRHVPIISDKYVDKDFGTGVLKISPGHDHNDYLLARKLGLP 360
           +DDRYSKYVG  AIVPMTYGRHVPII+DKYVDKDFGTGVLKISPGHDHNDYLLARKLGLP
Sbjct: 301 EDDRYSKYVGQTAIVPMTYGRHVPIIADKYVDKDFGTGVLKISPGHDHNDYLLARKLGLP 360

Query: 361 ILNVMNKDGTLNKVAGLYRGLDRFEARKKLWADLEETGLAVRKEAHTLRVPRSQRGGEII 420
           ILNVMNKD TLN VAGL+ GLDRFE R+KLWADLEE GLAV+KE HTLRVPRSQRGGE+I
Sbjct: 361 ILNVMNKDATLNDVAGLFCGLDRFEVREKLWADLEEIGLAVKKEPHTLRVPRSQRGGEVI 420

Query: 421 EPLVSKQWFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHR 480
           EPLVSKQWF+ M+PLAEKAL AVE  +LTIIPERFEKIYNHWL+NIKDWCISRQLWWGHR
Sbjct: 421 EPLVSKQWFVHMDPLAEKALLAVENKELTIIPERFEKIYNHWLTNIKDWCISRQLWWGHR 480

Query: 481 IPVWYVVGKNPEEEYIVARNAGEALEQAHKKYGKDVEIYQDPDVLDTWFSSALWPFSTLG 540
           IPVWYVVGK+ EE+YIVA++A EALE+A +KYGKDVEIYQDPDVLDTWFSS+LWPFSTLG
Sbjct: 481 IPVWYVVGKDCEEDYIVAKSAEEALEKALEKYGKDVEIYQDPDVLDTWFSSSLWPFSTLG 540

Query: 541 WPDETAEDFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGR 600
           WPD  A+DF  FYPT MLETGHDILFFWVARMVM+GIEFTGTVPFS++YLHGLIRDSQGR
Sbjct: 541 WPDVAAKDFNNFYPTNMLETGHDILFFWVARMVMMGIEFTGTVPFSHVYLHGLIRDSQGR 600

Query: 601 KMSKTLGNVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKF 660
           KMSK+LGNVIDPLDTIK+FGTDALRFT+ALGTAGQDLNLSTERLT+NKAFTNKLWNAGKF
Sbjct: 601 KMSKSLGNVIDPLDTIKDFGTDALRFTIALGTAGQDLNLSTERLTANKAFTNKLWNAGKF 660

Query: 661 ILQNLPTQNDSRSWESILSFEFEKEDCLLKLSLPECWIVSELHSLIDMVTTSYDKFFFGD 720
           +L +LP+ +D+ +WE++L  + +KE+ LL L LPECW VS+LH LID VT SY+K FFGD
Sbjct: 661 VLHSLPSLSDTSAWENLLDLKLDKEETLLSLPLPECWAVSKLHILIDSVTASYEKLFFGD 720

Query: 721 IGRDVYNFFWGDFADWYIEASKARLYQSGGHSVTLA-QAVLLYVFENILKLLHPFMPFVT 780
           +GR+ Y+FFW DFADWYIEASK+RLY SGG+SV+LA QAVLLYVFENILKLLHPFMPFVT
Sbjct: 721 VGRETYDFFWSDFADWYIEASKSRLYGSGGNSVSLASQAVLLYVFENILKLLHPFMPFVT 780

Query: 781 EELWQALPHRKEALIISPWPQSSLPRQASAVKKFENLKLLTRAIRNARAEYSVESAKRIS 812
           E+LWQALP+RKEALI+SPWPQ+SLPR   ++K+FENL+ LTRAIRNARAEYSVE  KRIS
Sbjct: 781 EDLWQALPYRKEALIVSPWPQNSLPRNVESIKRFENLQALTRAIRNARAEYSVEPVKRIS 840

BLAST of MS019170 vs. ExPASy Swiss-Prot
Match: Q8RBN5 (Valine--tRNA ligase OS=Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) OX=273068 GN=valS PE=3 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 5.9e-176
Identity = 346/904 (38.27%), Postives = 490/904 (54.20%), Query Frame = 0

Query: 59  EVAKTFDFAS-EERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 118
           ++AKT++    E+RIY +W  +G+F P  D +  PF I +PPPNVTG LHMGHA+  TL+
Sbjct: 3   DIAKTYNPKEFEDRIYSFWMEKGFFTPEIDPEKKPFTIVIPPPNVTGELHMGHALDNTLQ 62

Query: 119 ------------------------------------------------------------ 178
                                                                       
Sbjct: 63  DILIRWRRMQGYAALWIPGTDHASIATEIKVLDKIREETGKTKKDLTREEFLEKAWEWKD 122

Query: 179 ------------------------------------------------------------ 238
                                                                       
Sbjct: 123 KYENRILSQLKKLGSSCDWTRTAFTMDEKRSKAVREVFVSLYEKGLIYKGNRIINWCPSC 182

Query: 239 -------EVEYSEESGTLYYIKYRVAGGSKSDYLTIATTRPETLFGDVAIAVNPQDDRYS 298
                  EVE+ E  G L+YIKY V G  + DY+ IATTRPET+ GDVA+AV+P+D+RY 
Sbjct: 183 NTALSDAEVEHKEHKGHLWYIKYPVKG--EEDYVVIATTRPETMLGDVAVAVHPEDERYR 242

Query: 299 KYVGMMAIVPMTYGRHVPIISDKYVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMN 358
             +G   I+P+  GR +P+I+D+YVD  FGTG +K++P HD ND+ +  +  LP +N+MN
Sbjct: 243 HLIGKTLILPLV-GREIPVIADEYVDPSFGTGAVKVTPAHDPNDFEIGVRHNLPFVNIMN 302

Query: 359 KDGTLNKVAGLYRGLDRFEARKKLWADLEETGLAVRKEAHTLRVPRSQRGGEIIEPLVSK 418
           ++ T+N+  G Y GLDR+EAR+K+  DLEE GL ++ E H   V    R   ++EPL+S+
Sbjct: 303 ENATINENGGKYEGLDRYEAREKIVKDLEEQGLLLKVEEHVHNVGHCYRCDTVVEPLLSE 362

Query: 419 QWFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYV 478
           QWF+ MEPLA+ AL+ V++G +  +PERFEKIY +WL NIKDWCISRQLWWGHRIP WY 
Sbjct: 363 QWFVKMEPLAKPALQVVKEGKIKFVPERFEKIYTNWLENIKDWCISRQLWWGHRIPAWYC 422

Query: 479 VGKNPEEEYIVARNAGEALEQAHKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETA 538
                     V+R      E           I+QD +VLDTWFSSALWPFST+GWP+ET 
Sbjct: 423 EDCG---HVTVSREDPVKCEVC-----GSTNIHQDENVLDTWFSSALWPFSTMGWPEET- 482

Query: 539 EDFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTL 598
           ED K FYPT +L TG+DI+FFWVARM+ + +EF   +PF Y+ +HGL+RD+ GRKMSK+L
Sbjct: 483 EDLKYFYPTDVLVTGYDIIFFWVARMIFMSLEFMKEIPFKYVLIHGLVRDALGRKMSKSL 542

Query: 599 GNVIDPLDTIKEFGTDALRFTLALGTA-GQDLNLSTERLTSNKAFTNKLWNAGKFILQNL 658
           GN IDPL+ I+++G D LRFTL +G A G D+  S E++  ++ F NKLWNA +++L NL
Sbjct: 543 GNGIDPLEVIEKYGADTLRFTLVIGNAPGNDMRFSWEKVEHSRNFANKLWNASRYVLLNL 602

Query: 659 PTQNDSRSWESILSFEFEKEDCLLKLSLPECWIVSELHSLIDMVTTSYDKFFFGDIGRDV 718
             +ND+  +             L  L+L + WI++  ++++  +T + +KF  G     +
Sbjct: 603 -DENDTNLY-------------LDNLALADKWILTRYNNIVKEITDNLEKFELGVAASKL 662

Query: 719 YNFFWGDFADWYIEASKARLYQSGGHSVTLAQAVLLYVFENILKLLHPFMPFVTEELWQA 778
           Y+F W +F DWYIE SK  LY     +  + ++VL YV +N L+LLHPFMPF+TEE+WQ 
Sbjct: 663 YDFVWSEFCDWYIELSKPVLYSDNEEAKKVTKSVLRYVLDNTLRLLHPFMPFITEEIWQN 722

Query: 779 LPHRKEALIISPWP--QSSLPRQASAVKKFENLKLLTRAIRNARAEYSVESAKRISASIV 811
           LPH  E+++++ WP  +  L  +  A KK E +    RAIRN RAE +V  +K+    I 
Sbjct: 723 LPHEGESIMVAEWPKYREDLNFEEDA-KKAEIIMEAIRAIRNIRAEANVSPSKKAKVIIA 782

BLAST of MS019170 vs. ExPASy Swiss-Prot
Match: Q72ZW8 (Valine--tRNA ligase OS=Bacillus cereus (strain ATCC 10987 / NRS 248) OX=222523 GN=valS PE=3 SV=1)

HSP 1 Score: 603.2 bits (1554), Expect = 4.3e-171
Identity = 339/901 (37.62%), Postives = 474/901 (52.61%), Query Frame = 0

Query: 56  TSPEVAKTFDFAS-EERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFV 115
           T   +   +D  S EE +Y+WW    YF    D+   P+ I +PPPNVTG LH+GHA   
Sbjct: 4   TEKNLPTKYDHMSVEEGLYQWWLEGKYFEATGDEKKQPYTIVIPPPNVTGKLHLGHAWDT 63

Query: 116 TLEEV------------------------------------------------------- 175
           TL+++                                                       
Sbjct: 64  TLQDILTRTKRMQGYDVLWLPGMDHAGIATQAKVEGKLREEGISRYDLGREKFLEKAWEW 123

Query: 176 -------------------EYSEE------------------------------------ 235
                              +YS E                                    
Sbjct: 124 KEEYASHIRQQWGKVGLGLDYSRERFTLDEGLSDAVNKVFVQLYEKGLIYRGEYIINWDP 183

Query: 236 ----------------SGTLYYIKYRVAGGSKSDYLTIATTRPETLFGDVAIAVNPQDDR 295
                            G  Y++ Y +  G  S ++ +ATTRPET+ GD A+AV+P+DDR
Sbjct: 184 ATRTALSDIEVIHKEVQGAFYHMNYPLTDG--SGHIRLATTRPETMLGDTAVAVHPEDDR 243

Query: 296 YSKYVGMMAIVPMTYGRHVPIISDKYVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNV 355
           Y   +G    +P+  GR +PII+D+YV+KDFGTGV+KI+P HD ND+ +  +  LP + V
Sbjct: 244 YKHLIGKTVTLPIV-GREIPIIADEYVEKDFGTGVVKITPAHDPNDFEVGNRHDLPRILV 303

Query: 356 MNKDGTLNKVAGLYRGLDRFEARKKLWADLEETGLAVRKEAHTLRVPRSQRGGEIIEPLV 415
           MN+DGT+N+ AG Y G+DRFE RK L  DL+E G+ V  E H   V  S+R G ++EP +
Sbjct: 304 MNEDGTMNEKAGKYNGMDRFECRKALVKDLQEAGVLVEIEPHMHSVGHSERSGAVVEPYL 363

Query: 416 SKQWFMTMEPLAEK--ALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIP 475
           S QWF+ M PLAEK  AL+  E+  +T +PERFE  Y  W+ NI DWCISRQLWWGHRIP
Sbjct: 364 STQWFVKMAPLAEKAVALQQKEEEKVTFVPERFENTYLRWMENIHDWCISRQLWWGHRIP 423

Query: 476 VWYVVGKNPEEEYIVARNAGEALEQAHKKYGKDVEIY-QDPDVLDTWFSSALWPFSTLGW 535
            WY   K   E Y+                  D+E + QD DVLDTWFSSALWPFSTLGW
Sbjct: 424 AWY--HKETGEVYVGTEAPA------------DIENWNQDNDVLDTWFSSALWPFSTLGW 483

Query: 536 PDETAEDFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRK 595
           P+E A DFKR+Y T  L TG+DI+FFWV+RM+  G+EFTG  PF  + +HGL+RD QGRK
Sbjct: 484 PNEDAADFKRYYSTDALVTGYDIIFFWVSRMIFQGLEFTGERPFKDVLIHGLVRDEQGRK 543

Query: 596 MSKTLGNVIDPLDTIKEFGTDALRFTLALGTA-GQDLNLSTERLTSNKAFTNKLWNAGKF 655
           MSK+LGN IDP++ I+++G DA+RF L+ G+A GQDL  S E++ S   F NK+WNA +F
Sbjct: 544 MSKSLGNGIDPMEVIEKYGADAMRFFLSTGSAPGQDLRFSMEKVESTWNFINKIWNASRF 603

Query: 656 ILQNLPTQNDSRSWESILSFEFEKEDCLLKLSLPECWIVSELHSLIDMVTTSYDKFFFGD 715
           +L N+               ++E+ D   + S+ + WI++ L+  I+ VT + DK+ FG+
Sbjct: 604 VLMNMD------------DMKYEEIDLTGEKSVADKWILTRLNETIESVTRNMDKYEFGE 663

Query: 716 IGRDVYNFFWGDFADWYIEASKARLYQSGGHSVTLAQAVLLYVFENILKLLHPFMPFVTE 775
            GR +YNF W DF DWYIE +K  LY     +    +++L YV +  ++LLHPFMPFVTE
Sbjct: 664 AGRSLYNFIWDDFCDWYIEMAKLPLYGEDEAAKKTTRSILAYVLDQTMRLLHPFMPFVTE 723

Query: 776 ELWQALPHRKEALIISPWPQSSLPRQ-ASAVKKFENLKLLTRAIRNARAEYSVESAKRIS 805
           ++WQ LPH  E++ ++ WP      Q A A  +   L  + R++RN RAE +   +K++ 
Sbjct: 724 KIWQHLPHEGESITVAAWPTVREDLQDAEAAAEMHLLVDIIRSVRNIRAEVNTPMSKKVQ 783

BLAST of MS019170 vs. ExPASy Swiss-Prot
Match: Q6HD68 (Valine--tRNA ligase OS=Bacillus thuringiensis subsp. konkukian (strain 97-27) OX=281309 GN=valS PE=3 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 1.3e-170
Identity = 337/901 (37.40%), Postives = 473/901 (52.50%), Query Frame = 0

Query: 56  TSPEVAKTFDFAS-EERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFV 115
           T   +   +D  S EE +Y+WW    YF    D+   P+ I +PPPNVTG LH+GHA   
Sbjct: 4   TEKNLPTKYDHMSVEEGLYQWWLEGKYFEATGDEKKQPYTIVIPPPNVTGKLHLGHAWDT 63

Query: 116 TLEEV------------------------------------------------------- 175
           TL+++                                                       
Sbjct: 64  TLQDILTRTKRMQGYDVLWLPGMDHAGIATQAKVEGKLREEGISRYDLGREKFLEKAWEW 123

Query: 176 -------------------EYSEE------------------------------------ 235
                              +YS E                                    
Sbjct: 124 KEEYASHIRQQWGKVGLGLDYSRERFTLDKGLSDAVNKVFVQLYEKGLIYRGEYIINWDP 183

Query: 236 ----------------SGTLYYIKYRVAGGSKSDYLTIATTRPETLFGDVAIAVNPQDDR 295
                            G  Y++ Y +  G  S ++ +ATTRPET+ GD A+AV+P+DDR
Sbjct: 184 ATRTALSDIEVIHKEVQGAFYHMNYPLTDG--SGHIRLATTRPETMLGDTAVAVHPEDDR 243

Query: 296 YSKYVGMMAIVPMTYGRHVPIISDKYVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNV 355
           Y   +G    +P+  GR +PII+D+YV+KDFGTGV+KI+P HD ND+ +  +  LP + V
Sbjct: 244 YKHLIGKTVTLPIV-GREIPIIADEYVEKDFGTGVVKITPAHDPNDFEVGNRHDLPRILV 303

Query: 356 MNKDGTLNKVAGLYRGLDRFEARKKLWADLEETGLAVRKEAHTLRVPRSQRGGEIIEPLV 415
           MN+DG++N+ AG Y G+DRFE RK L  DL+E G+ V  E H   V  S+R G ++EP +
Sbjct: 304 MNEDGSMNEKAGKYNGMDRFECRKALVKDLQEAGVLVEIEPHMHSVGHSERSGAVVEPYL 363

Query: 416 SKQWFMTMEPLAEKALRAVEKGD--LTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIP 475
           S QWF+ M PLAEKA+   +K +  +T +P+RFE  Y  W+ NI DWCISRQLWWGHRIP
Sbjct: 364 STQWFVKMAPLAEKAIELQQKEEEKVTFVPDRFENTYLRWMENIHDWCISRQLWWGHRIP 423

Query: 476 VWYVVGKNPEEEYIVARNAGEALEQAHKKYGKDVEIY-QDPDVLDTWFSSALWPFSTLGW 535
            WY   K   E Y+                  D+E + QD DVLDTWFSSALWPFSTLGW
Sbjct: 424 AWY--HKETGEVYVGTEAPA------------DIENWNQDNDVLDTWFSSALWPFSTLGW 483

Query: 536 PDETAEDFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRK 595
           P+E A DFKR+Y T  L TG+DI+FFWV+RM+  G+EFTG  PF  + +HGL+RD QGRK
Sbjct: 484 PNEDAADFKRYYSTDALVTGYDIIFFWVSRMIFQGLEFTGERPFKDVLIHGLVRDEQGRK 543

Query: 596 MSKTLGNVIDPLDTIKEFGTDALRFTLALGTA-GQDLNLSTERLTSNKAFTNKLWNAGKF 655
           MSK+LGN IDP+D I+++G DA+RF L+ G+A GQDL  S E++ S   F NK+WNA +F
Sbjct: 544 MSKSLGNGIDPMDVIEKYGADAMRFFLSTGSAPGQDLRFSMEKVESTWNFINKIWNASRF 603

Query: 656 ILQNLPTQNDSRSWESILSFEFEKEDCLLKLSLPECWIVSELHSLIDMVTTSYDKFFFGD 715
           +L N+               +FE+ D   + S+ + WI++ L+  I+ VT + DK+ FG+
Sbjct: 604 VLMNMD------------DMKFEEIDLTGEKSVADKWILTRLNETIESVTRNMDKYEFGE 663

Query: 716 IGRDVYNFFWGDFADWYIEASKARLYQSGGHSVTLAQAVLLYVFENILKLLHPFMPFVTE 775
            GR +YNF W DF DWYIE +K  LY     +    +++L YV +  ++LLHPFMPFVTE
Sbjct: 664 AGRSLYNFIWDDFCDWYIEMAKLPLYGEDEAAKKTTRSILSYVLDQTMRLLHPFMPFVTE 723

Query: 776 ELWQALPHRKEALIISPWPQSSLPRQ-ASAVKKFENLKLLTRAIRNARAEYSVESAKRIS 805
           ++WQ LPH  E++ ++ WP      Q   A  +   L  + R++RN RAE +   +K++ 
Sbjct: 724 KIWQHLPHEGESITVAAWPTVREDLQDTEAAAEMHLLVDIIRSVRNIRAEVNTPMSKKVQ 783

BLAST of MS019170 vs. ExPASy Swiss-Prot
Match: Q817R6 (Valine--tRNA ligase OS=Bacillus cereus (strain ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711) OX=226900 GN=valS PE=3 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 1.7e-170
Identity = 337/901 (37.40%), Postives = 474/901 (52.61%), Query Frame = 0

Query: 56  TSPEVAKTFDFAS-EERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFV 115
           T   +   +D  S EE +Y+WW    YF    D+   P+ I +PPPNVTG LH+GHA   
Sbjct: 4   TEKNLPTKYDHMSVEEGLYQWWLEGKYFEATGDEKKQPYTIVIPPPNVTGKLHLGHAWDT 63

Query: 116 TLEEV------------------------------------------------------- 175
           TL+++                                                       
Sbjct: 64  TLQDILTRTKRMQGYDVLWLPGMDHAGIATQAKVEGKLREEGISRYDLGREKFLEKAWEW 123

Query: 176 -------------------EYSEE------------------------------------ 235
                              +YS E                                    
Sbjct: 124 KEEYASHIRQQWGKVGLGLDYSRERFTLDKGLSDAVNKVFVQLYEKGLIYRGEYIINWDP 183

Query: 236 ----------------SGTLYYIKYRVAGGSKSDYLTIATTRPETLFGDVAIAVNPQDDR 295
                            G  Y++ Y +  G  S ++ +ATTRPET+ GD A+AV+P+DDR
Sbjct: 184 ATRTALSDIEVIHKEVQGAFYHMNYPLTDG--SGHIRLATTRPETMLGDTAVAVHPEDDR 243

Query: 296 YSKYVGMMAIVPMTYGRHVPIISDKYVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNV 355
           Y   +G    +P+  GR +PII+D+YV+KDFGTGV+KI+P HD ND+ +  +  LP + V
Sbjct: 244 YKHLIGKTVTLPIV-GREIPIIADEYVEKDFGTGVVKITPAHDPNDFEVGNRHDLPRILV 303

Query: 356 MNKDGTLNKVAGLYRGLDRFEARKKLWADLEETGLAVRKEAHTLRVPRSQRGGEIIEPLV 415
           MN+DG++N+ AG Y G+DRFE RK+L  DL+E G+ V  E H   V  S+R G ++EP +
Sbjct: 304 MNEDGSMNEKAGKYNGMDRFECRKELVKDLQEAGVLVEIEPHMHSVGHSERSGAVVEPYL 363

Query: 416 SKQWFMTMEPLAEK--ALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIP 475
           S QWF+ M PLAEK  AL+  E+  +T +PERFE  Y  W+ NI DWCISRQLWWGHRIP
Sbjct: 364 STQWFVKMAPLAEKAVALQQKEEEKVTFVPERFENTYLRWMENIHDWCISRQLWWGHRIP 423

Query: 476 VWYVVGKNPEEEYIVARNAGEALEQAHKKYGKDVEIY-QDPDVLDTWFSSALWPFSTLGW 535
            WY   K   E Y+                  D+E + QD DVLDTWFSSALWPFSTLGW
Sbjct: 424 AWY--HKETGEVYVGTEAPA------------DIENWNQDNDVLDTWFSSALWPFSTLGW 483

Query: 536 PDETAEDFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRK 595
           P+E + DFKR+Y T  L TG+DI+FFWV+RM+  G+EFTG  PF  + +HGL+RD QGRK
Sbjct: 484 PNEDSADFKRYYSTDALVTGYDIIFFWVSRMIFQGLEFTGERPFKDVLIHGLVRDEQGRK 543

Query: 596 MSKTLGNVIDPLDTIKEFGTDALRFTLALGTA-GQDLNLSTERLTSNKAFTNKLWNAGKF 655
           MSK+LGN IDP+D I+++G DA+RF L+ G+A GQDL  S E++ S   F NK+WNA +F
Sbjct: 544 MSKSLGNGIDPMDVIEKYGADAMRFFLSTGSAPGQDLRFSMEKVESTWNFINKIWNASRF 603

Query: 656 ILQNLPTQNDSRSWESILSFEFEKEDCLLKLSLPECWIVSELHSLIDMVTTSYDKFFFGD 715
           +L N+               ++E+ D   + S+ + WI++ L+  I+ VT + DK+ FG+
Sbjct: 604 VLMNMD------------DMKYEEIDLTGEKSVADKWILTRLNETIESVTRNMDKYEFGE 663

Query: 716 IGRDVYNFFWGDFADWYIEASKARLYQSGGHSVTLAQAVLLYVFENILKLLHPFMPFVTE 775
            GR +YNF W DF DWYIE +K  LY     +    +++L YV +  ++LLHPFMPFVTE
Sbjct: 664 AGRSLYNFIWDDFCDWYIEMAKLPLYGEDEAAKKTTRSILAYVLDQTMRLLHPFMPFVTE 723

Query: 776 ELWQALPHRKEALIISPWPQSSLPRQ-ASAVKKFENLKLLTRAIRNARAEYSVESAKRIS 805
           ++WQ LPH  E++ ++ WP      Q   A  +   L  + R++RN RAE +   +K++ 
Sbjct: 724 KIWQHLPHEGESITVAAWPTVREDLQDTEAAAEMHLLVDIIRSVRNIRAEVNTPMSKKVQ 783

BLAST of MS019170 vs. ExPASy TrEMBL
Match: A0A6J1CQF3 (Valyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111013225 PE=3 SV=1)

HSP 1 Score: 1575.5 bits (4078), Expect = 0.0e+00
Identity = 812/966 (84.06%), Postives = 813/966 (84.16%), Query Frame = 0

Query: 1   MLLQMPFSGPSLLPPYRLNPLLFTRHRPAFRLSRSHFRRLRPTQFTVAASANGVFTSPEV 60
           MLLQMPFSGPSLLPPYRLNPLLFTRHRPAFRLSRSHF RLRPTQFTVAASANGVFTSPEV
Sbjct: 1   MLLQMPFSGPSLLPPYRLNPLLFTRHRPAFRLSRSHFCRLRPTQFTVAASANGVFTSPEV 60

Query: 61  AKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE--- 120
           AKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE   
Sbjct: 61  AKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLEDIM 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 VRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEKYGG 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 TITNQIKRLGASCDWTREHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPSLQTAV 240

Query: 241 ---EVEYSEESGTLYYIKYRVAGGSKSDYLTIATTRPETLFGDVAIAVNPQDDRYSKYVG 300
              EVEYSEESGTLYYIKYRVAGGSKSDYLTIATTRPETLFGDVAIAVNPQDDRYSKYVG
Sbjct: 241 SDLEVEYSEESGTLYYIKYRVAGGSKSDYLTIATTRPETLFGDVAIAVNPQDDRYSKYVG 300

Query: 301 MMAIVPMTYGRHVPIISDKYVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNKDGT 360
           MMAIVPMTYGRHVPIISDK+VDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNKDGT
Sbjct: 301 MMAIVPMTYGRHVPIISDKHVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNKDGT 360

Query: 361 LNKVAGLYRGLDRFEARKKLWADLEETGLAVRKEAHTLRVPRSQRGGEIIEPLVSKQWFM 420
           LNKVAGLYRGLDRFEARKKLWADLEETGLAVRKEAHTLRVPRSQRGGEIIEPLVSKQWFM
Sbjct: 361 LNKVAGLYRGLDRFEARKKLWADLEETGLAVRKEAHTLRVPRSQRGGEIIEPLVSKQWFM 420

Query: 421 TMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYVVGKN 480
           TMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYVVGKN
Sbjct: 421 TMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYVVGKN 480

Query: 481 PEEEYIVARNAGEALEQAHKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAEDFK 540
           PEEEYIVARNAGEALEQAHKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAEDFK
Sbjct: 481 PEEEYIVARNAGEALEQAHKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAEDFK 540

Query: 541 RFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLGNVI 600
           RFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLGNVI
Sbjct: 541 RFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLGNVI 600

Query: 601 DPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPTQND 660
           DPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPTQND
Sbjct: 601 DPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPTQND 660

Query: 661 SRSWESILSFEFEKEDCLLKLSLPECWIVSELHSLIDMVTTSYDKFFFGDIGRDVYNFFW 720
           SRSWESILSFEFEKEDCLLKLSLPECWIVSELHSLIDMVTTSYDKFFFGDIGRDVYNFFW
Sbjct: 661 SRSWESILSFEFEKEDCLLKLSLPECWIVSELHSLIDMVTTSYDKFFFGDIGRDVYNFFW 720

Query: 721 GDFADWYIEASKARLYQSGGHSVTLAQAVLLYVFENILKLLHPFMPFVTEELWQALPHRK 780
           GDFADWYIEASKARLYQSGGHSVTLAQAVLLYVFENILKLLHPFMPFVTEELWQALPHRK
Sbjct: 721 GDFADWYIEASKARLYQSGGHSVTLAQAVLLYVFENILKLLHPFMPFVTEELWQALPHRK 780

Query: 781 EALIISPWPQSSLPRQASAVKKFENLKLLTRAIRNARAEYSVESAKRISASIVASEEVNQ 818
           EALIISPWPQSSLPRQASAVKKFENLKLLTRAIRNARAEYSVESAKRISASIVASEEVNQ
Sbjct: 781 EALIISPWPQSSLPRQASAVKKFENLKLLTRAIRNARAEYSVESAKRISASIVASEEVNQ 840

BLAST of MS019170 vs. ExPASy TrEMBL
Match: A0A6J1IZ88 (Valyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111482028 PE=3 SV=1)

HSP 1 Score: 1481.5 bits (3834), Expect = 0.0e+00
Identity = 765/968 (79.03%), Postives = 787/968 (81.30%), Query Frame = 0

Query: 1   MLLQMPFSGPSLLPP---YRLNPLLFTRHRPAFRLSRSHFRRLRPTQFTVAASANGVFTS 60
           MLLQM FSGPSLL P   YRLNPL+FT+ R +F LSR HFRRL+  Q  VAASANGVFTS
Sbjct: 1   MLLQMAFSGPSLLHPSSAYRLNPLIFTKCRRSFGLSRFHFRRLQQRQLAVAASANGVFTS 60

Query: 61  PEVAKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 120
           PE+AKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE
Sbjct: 61  PEIAKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPHLQ 240

Query: 241 ------EVEYSEESGTLYYIKYRVAGGSKSDYLTIATTRPETLFGDVAIAVNPQDDRYSK 300
                 EVEYSEESGTLY+IKYRVAGGS+SDYLT+ATTRPETLFGDVAIAV+PQDDRYSK
Sbjct: 241 TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQDDRYSK 300

Query: 301 YVGMMAIVPMTYGRHVPIISDKYVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 360
           YVGMMAIVPMTYGRHVPIISDK VDKDFGTGVLKISPGHDHNDYLLARK GLPILNVMNK
Sbjct: 301 YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKFGLPILNVMNK 360

Query: 361 DGTLNKVAGLYRGLDRFEARKKLWADLEETGLAVRKEAHTLRVPRSQRGGEIIEPLVSKQ 420
           DGTLNKVAGLY GLDRFEARKKLWADLEETGLAV+KEAHTLRVPRSQRGGEIIEPLVSKQ
Sbjct: 361 DGTLNKVAGLYCGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ 420

Query: 421 WFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYVV 480
           WFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWY+V
Sbjct: 421 WFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV 480

Query: 481 GKNPEEEYIVARNAGEALEQAHKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 540
           GKNPEE+YIVARNA EA EQAHKKYGK VEIYQDPDVLDTWFSSALWPFSTLGWPDETAE
Sbjct: 481 GKNPEEDYIVARNADEAHEQAHKKYGKGVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 540

Query: 541 DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 600
           DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG
Sbjct: 541 DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 600

Query: 601 NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT 660
           NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT
Sbjct: 601 NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT 660

Query: 661 QNDSRSWESILSFEFEKEDCLLKLSLPECWIVSELHSLIDMVTTSYDKFFFGDIGRDVYN 720
           QNDS+SW+SILS+EFEKEDCLLKL LPECWIVSELHSLID+VT SYDKFFFGD+GRD+YN
Sbjct: 661 QNDSQSWKSILSYEFEKEDCLLKLPLPECWIVSELHSLIDVVTASYDKFFFGDVGRDIYN 720

Query: 721 FFWGDFADWYIEASKARLYQSGGHSVTLAQAVLLYVFENILKLLHPFMPFVTEELWQALP 780
           FFWGDFADWYIEASKARLYQSGG+SV LAQAVLLYVFENILKLLHPFMPFVTEELWQALP
Sbjct: 721 FFWGDFADWYIEASKARLYQSGGNSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP 780

Query: 781 HRKEALIISPWPQSSLPRQASAVKKFENLKLLTRAIRNARAEYSVESAKRISASIVASEE 817
           HRKEALIISPWPQ  LPRQA AVKKFENLKLLT+AIRNARAEYSVE AKRISASIVASEE
Sbjct: 781 HRKEALIISPWPQILLPRQAGAVKKFENLKLLTKAIRNARAEYSVEPAKRISASIVASEE 840

BLAST of MS019170 vs. ExPASy TrEMBL
Match: A0A6J1F580 (Valyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111442245 PE=3 SV=1)

HSP 1 Score: 1478.0 bits (3825), Expect = 0.0e+00
Identity = 764/968 (78.93%), Postives = 784/968 (80.99%), Query Frame = 0

Query: 1   MLLQMPFSGPSLLPP---YRLNPLLFTRHRPAFRLSRSHFRRLRPTQFTVAASANGVFTS 60
           MLLQM FSGPSLL P   YRLNPL+FT+ R +F LSR HFRRL   Q  VAASANGVFTS
Sbjct: 1   MLLQMAFSGPSLLHPSSAYRLNPLIFTKRRRSFGLSRFHFRRLLHRQLAVAASANGVFTS 60

Query: 61  PEVAKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 120
           PE+AKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE
Sbjct: 61  PEIAKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPHLQ 240

Query: 241 ------EVEYSEESGTLYYIKYRVAGGSKSDYLTIATTRPETLFGDVAIAVNPQDDRYSK 300
                 EVEYSEESGTLY+IKYRVAGGS+SDYLT+ATTRPETLFGDVAIAV+PQDDRYSK
Sbjct: 241 TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQDDRYSK 300

Query: 301 YVGMMAIVPMTYGRHVPIISDKYVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 360
           YVGMMAIVPMTYGRHVPIISDK VDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK
Sbjct: 301 YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 360

Query: 361 DGTLNKVAGLYRGLDRFEARKKLWADLEETGLAVRKEAHTLRVPRSQRGGEIIEPLVSKQ 420
           DGTLNKVAGLY GLDRFEARKKLW DLEETGLAV+KEAHTLRVPRSQRGGEIIEPLVSKQ
Sbjct: 361 DGTLNKVAGLYCGLDRFEARKKLWVDLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ 420

Query: 421 WFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYVV 480
           WFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWY+V
Sbjct: 421 WFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV 480

Query: 481 GKNPEEEYIVARNAGEALEQAHKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 540
           GKNPEE+YIVARNA EA EQAHKKYGK VEIYQDPDVLDTWFSSALWPFSTLGWPDETAE
Sbjct: 481 GKNPEEDYIVARNADEAHEQAHKKYGKGVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 540

Query: 541 DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 600
           DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG
Sbjct: 541 DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 600

Query: 601 NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT 660
           NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT
Sbjct: 601 NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT 660

Query: 661 QNDSRSWESILSFEFEKEDCLLKLSLPECWIVSELHSLIDMVTTSYDKFFFGDIGRDVYN 720
           QNDS+SW+SILS+EFEKEDCLLKL LPECWIVSELHS ID+VT SYDKFFFGD+GRD+YN
Sbjct: 661 QNDSQSWKSILSYEFEKEDCLLKLPLPECWIVSELHSQIDVVTASYDKFFFGDVGRDIYN 720

Query: 721 FFWGDFADWYIEASKARLYQSGGHSVTLAQAVLLYVFENILKLLHPFMPFVTEELWQALP 780
           FFWGDFADWYIEASKARLYQSGG SV LAQAVLLYVFENILKLLHPFMPFVTEELWQALP
Sbjct: 721 FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP 780

Query: 781 HRKEALIISPWPQSSLPRQASAVKKFENLKLLTRAIRNARAEYSVESAKRISASIVASEE 817
           HRKEALIISPWPQ  LPRQA AVKKFENLKLLT+AIRNARAEYSVE AKRISASIVASEE
Sbjct: 781 HRKEALIISPWPQILLPRQAGAVKKFENLKLLTKAIRNARAEYSVEPAKRISASIVASEE 840

BLAST of MS019170 vs. ExPASy TrEMBL
Match: A0A1S3C4H7 (Valyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103496791 PE=3 SV=1)

HSP 1 Score: 1457.2 bits (3771), Expect = 0.0e+00
Identity = 755/968 (78.00%), Postives = 782/968 (80.79%), Query Frame = 0

Query: 1   MLLQMPFSGPSLLPP---YRLNPLLFTRHRPAFRLSRSHFRRLRPTQFTVAASANGVFTS 60
           MLL M FSGPS L P    RLNPLLFT+HR +F LSR  FRRL   Q  VAASANGVFTS
Sbjct: 1   MLLLMAFSGPSFLHPSSASRLNPLLFTKHRRSFALSRFRFRRLHQRQLAVAASANGVFTS 60

Query: 61  PEVAKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 120
           PE+AKTFDFASEERIY+WWESQGYFRPH+DQD+VPFVISMPPPNVTGSLHMGHAMFVTLE
Sbjct: 61  PEIAKTFDFASEERIYKWWESQGYFRPHYDQDTVPFVISMPPPNVTGSLHMGHAMFVTLE 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ 240

Query: 241 ------EVEYSEESGTLYYIKYRVAGGSKSDYLTIATTRPETLFGDVAIAVNPQDDRYSK 300
                 EVEYSEESGTLY+IKYRVAGGS SDYLT+ATTRPETLFGDVAIAVNPQDDRYSK
Sbjct: 241 TAVSDLEVEYSEESGTLYHIKYRVAGGSSSDYLTVATTRPETLFGDVAIAVNPQDDRYSK 300

Query: 301 YVGMMAIVPMTYGRHVPIISDKYVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 360
           YVGM AIVPMTYGRHVPIISDK VDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK
Sbjct: 301 YVGMTAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 360

Query: 361 DGTLNKVAGLYRGLDRFEARKKLWADLEETGLAVRKEAHTLRVPRSQRGGEIIEPLVSKQ 420
           DGTLNKVAGLY GLDRFEARKKLW DLEE GLAV+KEAHTLRVPRSQRGGEIIEPLVSKQ
Sbjct: 361 DGTLNKVAGLYCGLDRFEARKKLWTDLEEMGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ 420

Query: 421 WFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYVV 480
           WF+TMEPLAEKALRAVEKG+LTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWY+V
Sbjct: 421 WFVTMEPLAEKALRAVEKGELTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV 480

Query: 481 GKNPEEEYIVARNAGEALEQAHKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 540
           GKNPEE+YIVARNA EALEQA KKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE
Sbjct: 481 GKNPEEDYIVARNADEALEQAQKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 540

Query: 541 DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 600
           DFKRFYPTTMLETGHDILFFWVARMVM+GIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG
Sbjct: 541 DFKRFYPTTMLETGHDILFFWVARMVMMGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 600

Query: 601 NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT 660
           NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT
Sbjct: 601 NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT 660

Query: 661 QNDSRSWESILSFEFEKEDCLLKLSLPECWIVSELHSLIDMVTTSYDKFFFGDIGRDVYN 720
           QNDS+SW+SILSFEFEK+DCLLKL LPECWIVSELHSLID+VT SYDKFFFGD+GRDVYN
Sbjct: 661 QNDSQSWDSILSFEFEKDDCLLKLPLPECWIVSELHSLIDVVTISYDKFFFGDVGRDVYN 720

Query: 721 FFWGDFADWYIEASKARLYQSGGHSVTLAQAVLLYVFENILKLLHPFMPFVTEELWQALP 780
           FFWGDFADWYIEASKARLYQSGG SV LAQAVLLYVF+NILKLLHPFMPFVTEELWQALP
Sbjct: 721 FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFKNILKLLHPFMPFVTEELWQALP 780

Query: 781 HRKEALIISPWPQSSLPRQASAVKKFENLKLLTRAIRNARAEYSVESAKRISASIVASEE 817
           +RKEALIIS WPQ SLPRQASAVKKFENLKLLT+AIRNARAEYSVE AKRISASIVASEE
Sbjct: 781 NRKEALIISHWPQISLPRQASAVKKFENLKLLTKAIRNARAEYSVEPAKRISASIVASEE 840

BLAST of MS019170 vs. ExPASy TrEMBL
Match: A0A1S3C571 (Valyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103496791 PE=3 SV=1)

HSP 1 Score: 1400.6 bits (3624), Expect = 0.0e+00
Identity = 724/948 (76.37%), Postives = 754/948 (79.54%), Query Frame = 0

Query: 1   MLLQMPFSGPSLLPP---YRLNPLLFTRHRPAFRLSRSHFRRLRPTQFTVAASANGVFTS 60
           MLL M FSGPS L P    RLNPLLFT+HR +F LSR  FRRL   Q  VAASANGVFTS
Sbjct: 1   MLLLMAFSGPSFLHPSSASRLNPLLFTKHRRSFALSRFRFRRLHQRQLAVAASANGVFTS 60

Query: 61  PEVAKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 120
           PE+AKTFDFASEERIY+WWESQGYFRPH+DQD+VPFVISMPPPNVTGSLHMGHAMFVTLE
Sbjct: 61  PEIAKTFDFASEERIYKWWESQGYFRPHYDQDTVPFVISMPPPNVTGSLHMGHAMFVTLE 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ 240

Query: 241 ------EVEYSEESGTLYYIKYRVAGGSKSDYLTIATTRPETLFGDVAIAVNPQDDRYSK 300
                 EVEYSEESGTLY+IKYRVAGGS SDYLT+ATTRPETLFGDVAIAVNPQDDRYSK
Sbjct: 241 TAVSDLEVEYSEESGTLYHIKYRVAGGSSSDYLTVATTRPETLFGDVAIAVNPQDDRYSK 300

Query: 301 YVGMMAIVPMTYGRHVPIISDKYVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 360
           YVGM AIVPMTYGRHVPIISDK VDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK
Sbjct: 301 YVGMTAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 360

Query: 361 DGTLNKVAGLYRGLDRFEARKKLWADLEETGLAVRKEAHTLRVPRSQRGGEIIEPLVSKQ 420
           DGTLNKVAGLY GLDRFEARKKLW DLEE GLAV+KEAHTLRVPRSQRGGEIIEPLVSKQ
Sbjct: 361 DGTLNKVAGLYCGLDRFEARKKLWTDLEEMGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ 420

Query: 421 WFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYVV 480
           WF+TMEPLAEKALRAVEKG+LTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWY+V
Sbjct: 421 WFVTMEPLAEKALRAVEKGELTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV 480

Query: 481 GKNPEEEYIVARNAGEALEQAHKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 540
           GKNPEE+YIVARNA EALEQA KKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE
Sbjct: 481 GKNPEEDYIVARNADEALEQAQKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 540

Query: 541 DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 600
           DFKRFYPTTMLETGHDILFFWVARMVM+GIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG
Sbjct: 541 DFKRFYPTTMLETGHDILFFWVARMVMMGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 600

Query: 601 NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT 660
           NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT
Sbjct: 601 NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT 660

Query: 661 QNDSRSWESILSFEFEKEDCLLKLSLPECWIVSELHSLIDMVTTSYDKFFFGDIGRDVYN 720
           QNDS+SW+SILSFEFEK+DCLLKL LPECWIVSELHSLID+VT SYDKFFFGD+GRDVYN
Sbjct: 661 QNDSQSWDSILSFEFEKDDCLLKLPLPECWIVSELHSLIDVVTISYDKFFFGDVGRDVYN 720

Query: 721 FFWGDFADWYIEASKARLYQSGGHSVTLAQAVLLYVFENILKLLHPFMPFVTEELWQALP 780
           FFWGDFADWYIEASKARLYQSGG SV LAQAVLLYVF+NILKLLHPFMPFVTEELWQALP
Sbjct: 721 FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFKNILKLLHPFMPFVTEELWQALP 780

Query: 781 HRKEALIISPWPQSSLPRQASAVKKFENLKLLTRAIRNARAEYSVESAKRISASIVASEE 817
           +RKEALIIS WPQ SLPRQASAVKKFENLKLL                K + A +   + 
Sbjct: 781 NRKEALIISHWPQISLPRQASAVKKFENLKLL--------------GEKEVLALLTRLDL 840

BLAST of MS019170 vs. TAIR 10
Match: AT5G16715.1 (ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases )

HSP 1 Score: 1208.0 bits (3124), Expect = 0.0e+00
Identity = 630/971 (64.88%), Postives = 715/971 (73.64%), Query Frame = 0

Query: 1   MLLQMPFSGPS-----LLP--PYRLNPLLFTRHRPAFRLSRSHFRRL---RPTQFTVAAS 60
           M+L+  FS P+     L P  P++LN L FTR R    +S S    +   R   F+ AAS
Sbjct: 1   MILKTAFSLPTPTTTLLSPSSPHQLNTLFFTRRRRRL-ISPSRLNSIFSQRRFSFSAAAS 60

Query: 61  ANGVFTSPEVAKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGH 120
            N VFTSPE +KTFDF+SEE+IY+WWESQGYF+P+FDQ   PFVI MPPPNVTGSLHMGH
Sbjct: 61  GNNVFTSPETSKTFDFSSEEKIYKWWESQGYFKPNFDQGGSPFVIPMPPPNVTGSLHMGH 120

Query: 121 AMFVTLE----------------------------------------------------- 180
           AMFVTLE                                                     
Sbjct: 121 AMFVTLEDIMVRYNRMNGRPTLWLPGTDHAGIATQLVVEKMLASEGIKRVDLGRDEFTKR 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 VWEWKEKYGGTITNQIKRLGASCDWSRERFTLDEQLSRAVVEAFVKLHDKGLIYQGSYMV 240

Query: 241 -------------EVEYSEESGTLYYIKYRVAGGSKSDYLTIATTRPETLFGDVAIAVNP 300
                        EVEYSEE G LY+IKYRVAG    D+LTIATTRPETLFGDVA+AV+P
Sbjct: 241 NWSPNLQTAVSDLEVEYSEEPGFLYHIKYRVAG--SPDFLTIATTRPETLFGDVALAVHP 300

Query: 301 QDDRYSKYVGMMAIVPMTYGRHVPIISDKYVDKDFGTGVLKISPGHDHNDYLLARKLGLP 360
           +DDRYSKYVG  AIVPMTYGRHVPII+DKYVDKDFGTGVLKISPGHDHNDYLLARKLGLP
Sbjct: 301 EDDRYSKYVGQTAIVPMTYGRHVPIIADKYVDKDFGTGVLKISPGHDHNDYLLARKLGLP 360

Query: 361 ILNVMNKDGTLNKVAGLYRGLDRFEARKKLWADLEETGLAVRKEAHTLRVPRSQRGGEII 420
           ILNVMNKD TLN VAGL+ GLDRFE R+KLWADLEE GLAV+KE HTLRVPRSQRGGE+I
Sbjct: 361 ILNVMNKDATLNDVAGLFCGLDRFEVREKLWADLEEIGLAVKKEPHTLRVPRSQRGGEVI 420

Query: 421 EPLVSKQWFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHR 480
           EPLVSKQWF+ M+PLAEKAL AVE  +LTIIPERFEKIYNHWL+NIKDWCISRQLWWGHR
Sbjct: 421 EPLVSKQWFVHMDPLAEKALLAVENKELTIIPERFEKIYNHWLTNIKDWCISRQLWWGHR 480

Query: 481 IPVWYVVGKNPEEEYIVARNAGEALEQAHKKYGKDVEIYQDPDVLDTWFSSALWPFSTLG 540
           IPVWYVVGK+ EE+YIVA++A EALE+A +KYGKDVEIYQDPDVLDTWFSS+LWPFSTLG
Sbjct: 481 IPVWYVVGKDCEEDYIVAKSAEEALEKALEKYGKDVEIYQDPDVLDTWFSSSLWPFSTLG 540

Query: 541 WPDETAEDFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGR 600
           WPD  A+DF  FYPT MLETGHDILFFWVARMVM+GIEFTGTVPFS++YLHGLIRDSQGR
Sbjct: 541 WPDVAAKDFNNFYPTNMLETGHDILFFWVARMVMMGIEFTGTVPFSHVYLHGLIRDSQGR 600

Query: 601 KMSKTLGNVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKF 660
           KMSK+LGNVIDPLDTIK+FGTDALRFT+ALGTAGQDLNLSTERLT+NKAFTNKLWNAGKF
Sbjct: 601 KMSKSLGNVIDPLDTIKDFGTDALRFTIALGTAGQDLNLSTERLTANKAFTNKLWNAGKF 660

Query: 661 ILQNLPTQNDSRSWESILSFEFEKEDCLLKLSLPECWIVSELHSLIDMVTTSYDKFFFGD 720
           +L +LP+ +D+ +WE++L  + +KE+ LL L LPECW VS+LH LID VT SY+K FFGD
Sbjct: 661 VLHSLPSLSDTSAWENLLDLKLDKEETLLSLPLPECWAVSKLHILIDSVTASYEKLFFGD 720

Query: 721 IGRDVYNFFWGDFADWYIEASKARLYQSGGHSVTLA-QAVLLYVFENILKLLHPFMPFVT 780
           +GR+ Y+FFW DFADWYIEASK+RLY SGG+SV+LA QAVLLYVFENILKLLHPFMPFVT
Sbjct: 721 VGRETYDFFWSDFADWYIEASKSRLYGSGGNSVSLASQAVLLYVFENILKLLHPFMPFVT 780

Query: 781 EELWQALPHRKEALIISPWPQSSLPRQASAVKKFENLKLLTRAIRNARAEYSVESAKRIS 812
           E+LWQALP+RKEALI+SPWPQ+SLPR   ++K+FENL+ LTRAIRNARAEYSVE  KRIS
Sbjct: 781 EDLWQALPYRKEALIVSPWPQNSLPRNVESIKRFENLQALTRAIRNARAEYSVEPVKRIS 840

BLAST of MS019170 vs. TAIR 10
Match: AT1G14610.1 (valyl-tRNA synthetase / valine--tRNA ligase (VALRS) )

HSP 1 Score: 415.2 bits (1066), Expect = 1.2e-115
Identity = 270/767 (35.20%), Postives = 387/767 (50.46%), Query Frame = 0

Query: 124  ESGTLYYIKYRVAGGSKSDYLTIATTRPETLFGDVAIAVNPQDDRYSKYVGMMAIVPMTY 183
            E G L    Y + GG     + +ATTR ET+ GD AIA++P D RY    G  A+ P   
Sbjct: 344  EFGLLTSFAYPLEGGLGE--VIVATTRVETMLGDTAIAIHPDDARYKHLHGKFAVHPFN- 403

Query: 184  GRHVPIISDK-YVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNKDGTLNKVAGL- 243
            GR +PII D   VD +FGTG +KI+P HD ND  + ++  L  +N+   DG +N   G  
Sbjct: 404  GRKLPIICDGILVDPNFGTGCVKITPAHDPNDCEVGKRHKLEFINIFTDDGKINTNGGSD 463

Query: 244  YRGLDRFEARKKLWADLEETGLAVRKEAHTLRVPRSQRGGEIIEPLVSKQWFMTMEPLAE 303
            + G+ RF AR+ +   L++ GL    + + +R+    R  ++IEP++  QW++    + +
Sbjct: 464  FAGMPRFAAREAVVEALQKQGLYRGAKNNEMRLGLCSRTNDVIEPMIKPQWYVNCSMIGK 523

Query: 304  KALRAV---EKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYVVGKNPE-- 363
            +AL      E   L  +P+++   +  WL NI+DWCISRQLWWGHRIP WY   +  +  
Sbjct: 524  EALDVAITDENKKLEFVPKQYTAEWRRWLENIRDWCISRQLWWGHRIPAWYATLEEDQLK 583

Query: 364  ------EEYIVARNAGEALEQAHKKY-GKDVEIYQDPDVLDTWFSSALWPFSTLGWPDET 423
                  + ++VAR   +A E+A +K+ GK  E+ +DPDVLDTWFSS L+P S LGWPD T
Sbjct: 584  EVGAYSDHWVVARTEDDAREEAAQKFLGKKFELTRDPDVLDTWFSSGLFPLSVLGWPDVT 643

Query: 424  AEDFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKT 483
             +DFK FYPT++LETGHDILFFWVARMVM+G++  G VPFS +Y H +IRD+ GRKMSK+
Sbjct: 644  -DDFKAFYPTSVLETGHDILFFWVARMVMMGMKLGGEVPFSKVYFHPMIRDAHGRKMSKS 703

Query: 484  LGNVIDPLDTIK-------------------------------------EFGTDALRFTL 543
            LGNVIDPL+ I                                      E GTDALRF L
Sbjct: 704  LGNVIDPLEVINGVTLEGLHKRLEEGNLDPKEVIVAKEGQVKDFPNGIPECGTDALRFAL 763

Query: 544  ALGTAGQD-LNLSTERLTSNKAFTNKLWNAGKFILQNLPTQNDSRSWESILSFEFEKEDC 603
               TA  D +NL   R+   + + NKLWNA +F +  L    D  +    LS E     C
Sbjct: 764  VSYTAQSDKINLDILRVVGYRQWCNKLWNAVRFAMMKL---GDGYTPPQTLSPETMPFSC 823

Query: 604  LLKLSLPECWIVSELHSLIDMVTTSYDKFFFGDIGRDVYNFFWGDFADWYIEASKARLYQ 663
                     WI+S L+  I     S D F F D    +Y ++   F D YIEA K   Y 
Sbjct: 824  Q--------WILSVLNKAISKTVVSLDAFEFSDAANTIYAWWQYQFCDVYIEAIKP--YF 883

Query: 664  SGGHSV-----TLAQAVLLYVFENILKLLHPFMPFVTEELWQALP-----HRKEALIISP 723
            +G +         AQ  L    E  L+LLHPFMPFVTEELWQ LP      RK +++I  
Sbjct: 884  AGDNPTFASERAHAQHALWISLETGLRLLHPFMPFVTEELWQRLPAPKDTERKASIMICD 943

Query: 724  WP-----------QSSLPRQASAVKKFENLKL-LTRAIRNARAEYSVESAKRISASIVAS 783
            +P           +S +    + VK    L+  L    +N R          +++ IV S
Sbjct: 944  YPSAIENWSNEKVESEMDTVLATVKCMRALRAGLLEKQKNERLPAFALCENNVTSEIVKS 1003

Query: 784  EEVN-----QYISVRNVDQSVHL--------VAGEGLEAYLPLADMVDISVELQRLSKRL 804
             E+         S+  V +  H            E L+ YL +   ++   E +++  ++
Sbjct: 1004 HELEIRTLANLSSLEVVSKGQHAAPPGSSVETVNENLKVYLEVDGAINTEAEQEKIRNKI 1063

HSP 2 Score: 60.1 bits (144), Expect = 9.7e-09
Identity = 27/63 (42.86%), Postives = 39/63 (61.90%), Query Frame = 0

Query: 57  SPEVAKTFDFASEERI-YRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVT 116
           S ++AK +  A+ E+  Y WWE    F+        PFVI +PPPNVTG+LH+GHA+   
Sbjct: 133 SSQMAKQYSPATVEKSWYAWWEKSDLFKADAKSSKPPFVIVLPPPNVTGALHIGHALTSA 192

Query: 117 LEE 119
           +E+
Sbjct: 193 IED 195

BLAST of MS019170 vs. TAIR 10
Match: AT5G49030.1 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 157.9 bits (398), Expect = 3.4e-38
Identity = 123/493 (24.95%), Postives = 218/493 (44.22%), Query Frame = 0

Query: 189 IISDKYVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNKDGTLNKVAGLYRGLDRF 248
           +I   Y+  + GTG++  +PGH   DY    K GLP+++ ++ +G   + AG +RGL   
Sbjct: 438 VIGGDYITTESGTGLVHTAPGHGQEDYATGLKYGLPLVSPVDDEGKFTEEAGQFRGLSVL 497

Query: 249 -EARKKLWADLEETGLAVRKEAHTLRVPRSQRGGEIIEPLVSKQWFMTMEPLAEKALRAV 308
            E    + + L+E    V +E++  + P   R  +      ++QWF ++E      + A+
Sbjct: 498 GEGNTAVVSYLDENMSLVMEESYAHKYPYDWRTKKPTIFRATEQWFASVEGFRTATMDAI 557

Query: 309 EKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWY-VVGKNP---EE-----E 368
              ++  +P +     +   S+  DWCISRQ  WG  IP +Y V  K P   EE     +
Sbjct: 558 --NNVKWVPHQAVNRISAMTSSRSDWCISRQRTWGVPIPAFYHVKTKEPLMNEETINHVK 617

Query: 369 YIVARNAGEA---------LEQAHKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDET 428
            I+++   +A         L + ++    D E  +  D +D WF S         W    
Sbjct: 618 SIISQKGSDAWWYMSVEDLLPEKYRDKAADYE--KGTDTMDVWFDSG------SSWAGVL 677

Query: 429 AEDFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKT 488
            +     +P  +   G D    W    ++  I   G  P+S +  HG + D +G KMSK+
Sbjct: 678 GKREGLSFPADVYLEGTDQHRGWFQSSLLTSIATQGKAPYSAVITHGFVLDEKGMKMSKS 737

Query: 489 LGNVIDPLDTIK---------EFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWN 548
           LGNV+DP   I+          +G D +R  ++      D+ +  + L        KL  
Sbjct: 738 LGNVVDPRLVIEGGKNSKDAPAYGADVMRLWVSSVDYTGDVLIGPQILRQMSDIYRKLRG 797

Query: 549 AGKFILQNLPTQNDSRSWESILSFEFEKEDCLLKLSLPECWIVSELHSLIDMVTTSYDKF 608
             +++L NL        W    +  ++       L + +   + +L +++  +   Y+ +
Sbjct: 798 TLRYLLGNL------HDWRVDNAVPYQ------DLPIIDQHALFQLENVVKNIQECYENY 857

Query: 609 FFGDIGRDVYNFFWGDFADWYIEASKARLYQSGGHSVT--LAQAVLLYVFENILKLLHPF 652
            F  I + +  F   D +++Y + +K RLY  G  S T    Q VL     +IL+++ P 
Sbjct: 858 QFFKIFQIIQRFTIVDLSNFYFDIAKDRLYTGGTSSFTRRSCQTVLSTHLLSILRVIAPI 908

BLAST of MS019170 vs. TAIR 10
Match: AT5G49030.2 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 157.9 bits (398), Expect = 3.4e-38
Identity = 123/493 (24.95%), Postives = 218/493 (44.22%), Query Frame = 0

Query: 189 IISDKYVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNKDGTLNKVAGLYRGLDRF 248
           +I   Y+  + GTG++  +PGH   DY    K GLP+++ ++ +G   + AG +RGL   
Sbjct: 438 VIGGDYITTESGTGLVHTAPGHGQEDYATGLKYGLPLVSPVDDEGKFTEEAGQFRGLSVL 497

Query: 249 -EARKKLWADLEETGLAVRKEAHTLRVPRSQRGGEIIEPLVSKQWFMTMEPLAEKALRAV 308
            E    + + L+E    V +E++  + P   R  +      ++QWF ++E      + A+
Sbjct: 498 GEGNTAVVSYLDENMSLVMEESYAHKYPYDWRTKKPTIFRATEQWFASVEGFRTATMDAI 557

Query: 309 EKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWY-VVGKNP---EE-----E 368
              ++  +P +     +   S+  DWCISRQ  WG  IP +Y V  K P   EE     +
Sbjct: 558 --NNVKWVPHQAVNRISAMTSSRSDWCISRQRTWGVPIPAFYHVKTKEPLMNEETINHVK 617

Query: 369 YIVARNAGEA---------LEQAHKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDET 428
            I+++   +A         L + ++    D E  +  D +D WF S         W    
Sbjct: 618 SIISQKGSDAWWYMSVEDLLPEKYRDKAADYE--KGTDTMDVWFDSG------SSWAGVL 677

Query: 429 AEDFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKT 488
            +     +P  +   G D    W    ++  I   G  P+S +  HG + D +G KMSK+
Sbjct: 678 GKREGLSFPADVYLEGTDQHRGWFQSSLLTSIATQGKAPYSAVITHGFVLDEKGMKMSKS 737

Query: 489 LGNVIDPLDTIK---------EFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWN 548
           LGNV+DP   I+          +G D +R  ++      D+ +  + L        KL  
Sbjct: 738 LGNVVDPRLVIEGGKNSKDAPAYGADVMRLWVSSVDYTGDVLIGPQILRQMSDIYRKLRG 797

Query: 549 AGKFILQNLPTQNDSRSWESILSFEFEKEDCLLKLSLPECWIVSELHSLIDMVTTSYDKF 608
             +++L NL        W    +  ++       L + +   + +L +++  +   Y+ +
Sbjct: 798 TLRYLLGNL------HDWRVDNAVPYQ------DLPIIDQHALFQLENVVKNIQECYENY 857

Query: 609 FFGDIGRDVYNFFWGDFADWYIEASKARLYQSGGHSVT--LAQAVLLYVFENILKLLHPF 652
            F  I + +  F   D +++Y + +K RLY  G  S T    Q VL     +IL+++ P 
Sbjct: 858 QFFKIFQIIQRFTIVDLSNFYFDIAKDRLYTGGTSSFTRRSCQTVLSTHLLSILRVIAPI 908

BLAST of MS019170 vs. TAIR 10
Match: AT5G49030.3 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 157.9 bits (398), Expect = 3.4e-38
Identity = 123/493 (24.95%), Postives = 218/493 (44.22%), Query Frame = 0

Query: 189  IISDKYVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNKDGTLNKVAGLYRGLDRF 248
            +I   Y+  + GTG++  +PGH   DY    K GLP+++ ++ +G   + AG +RGL   
Sbjct: 624  VIGGDYITTESGTGLVHTAPGHGQEDYATGLKYGLPLVSPVDDEGKFTEEAGQFRGLSVL 683

Query: 249  -EARKKLWADLEETGLAVRKEAHTLRVPRSQRGGEIIEPLVSKQWFMTMEPLAEKALRAV 308
             E    + + L+E    V +E++  + P   R  +      ++QWF ++E      + A+
Sbjct: 684  GEGNTAVVSYLDENMSLVMEESYAHKYPYDWRTKKPTIFRATEQWFASVEGFRTATMDAI 743

Query: 309  EKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWY-VVGKNP---EE-----E 368
               ++  +P +     +   S+  DWCISRQ  WG  IP +Y V  K P   EE     +
Sbjct: 744  --NNVKWVPHQAVNRISAMTSSRSDWCISRQRTWGVPIPAFYHVKTKEPLMNEETINHVK 803

Query: 369  YIVARNAGEA---------LEQAHKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDET 428
             I+++   +A         L + ++    D E  +  D +D WF S         W    
Sbjct: 804  SIISQKGSDAWWYMSVEDLLPEKYRDKAADYE--KGTDTMDVWFDSG------SSWAGVL 863

Query: 429  AEDFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKT 488
             +     +P  +   G D    W    ++  I   G  P+S +  HG + D +G KMSK+
Sbjct: 864  GKREGLSFPADVYLEGTDQHRGWFQSSLLTSIATQGKAPYSAVITHGFVLDEKGMKMSKS 923

Query: 489  LGNVIDPLDTIK---------EFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWN 548
            LGNV+DP   I+          +G D +R  ++      D+ +  + L        KL  
Sbjct: 924  LGNVVDPRLVIEGGKNSKDAPAYGADVMRLWVSSVDYTGDVLIGPQILRQMSDIYRKLRG 983

Query: 549  AGKFILQNLPTQNDSRSWESILSFEFEKEDCLLKLSLPECWIVSELHSLIDMVTTSYDKF 608
              +++L NL        W    +  ++       L + +   + +L +++  +   Y+ +
Sbjct: 984  TLRYLLGNL------HDWRVDNAVPYQ------DLPIIDQHALFQLENVVKNIQECYENY 1043

Query: 609  FFGDIGRDVYNFFWGDFADWYIEASKARLYQSGGHSVT--LAQAVLLYVFENILKLLHPF 652
             F  I + +  F   D +++Y + +K RLY  G  S T    Q VL     +IL+++ P 
Sbjct: 1044 QFFKIFQIIQRFTIVDLSNFYFDIAKDRLYTGGTSSFTRRSCQTVLSTHLLSILRVIAPI 1094