MS019167 (gene) Bitter gourd (TR) v1

Overview


Name	MS019167
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	THO complex subunit 4D-like
Location	scaffold20: 2156196 .. 2157567 (-)
RNA-Seq Expression	MS019167
Synteny	MS019167

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTACGCCTGTGGATATCTCACGAGAGGCTGTGATAAAGAAGAATAACCGTAAGAAGCTCAGAGCACGACGTAGGGCCCGTTGTGGGCGTGGAGCAGGTGGGTCTTTTAATGGTGGAAGAGTAGTGATAGGGTCGGTTCGTAATCTCAACATAAATGCACGACCATCTGCTTGCTCAATTAGCTTTAAACTGTCGATATTTAATGCATATATATGCTTGAGAACCTGATATTCCTTGAAAATACATGCTCATGGGGATCTCAAATAACATTACTATAGTAACTCTAAAAGAAGATGATCAGTAAAATTGTTGTTCTATGGAGAATCTCATTGCGGCTCACTCTCTTATCGGAATCGTCATCTGGTGTATTTGTACCATTATCTTTGCAAATATTGAATACATGTGGGTTATATGGGTAACTTTCAAAGGACTGTCAAGAGTGGAGAATTATATTATTGTCGATTAATAAGCTGATATGTGTTTTCCCCATCTGGATTCTTATCAGCATGCTTTCATCAGCCCCCACGCAGAATGAAGAATGTTCAATGGCAGCATGATTTACTTGAAGACAGTCTTAGAGCTTCAGGGATTTCTGGAATTGAAATTGGCACAAAGTTGTACGTTTCCGACTTGAATTTTGGGGTGACCAAAGAAGATATAATGGTACAACTTAAGGCATATATATTTGACTAAAGATGATTTCGATTAATATTCTCTTGTAACCTTACGGTGAACTCAGGAGTTGTTCTCTGCTGTTGGAGACCTGAAAAGATTTGCAATTCATTATGACAAAAATGGCCGTCCATGTGTAAGTTCCTACACTATCTTTTTGTAAAACTTTCCAGTAATGTGTTGCTGTTTGGCTTTTTAGTTTTTCCCATATCAAGTATCAATGTTATTTTTGTAGTATAGACTCACAAAAATGGAGTGAGAGGATTCAAACTCATGATCTCTTGGTCAGAGATAGGTGTCAATTACCACTGAGCTAAGTTCATATTGTCGATACTAGAATACATGTTATGAATTCAAAAACCAACTTCTACTGAATGTAGGTTTAAACCCGTTGGTTTGGTAAGTTCTTTAGATTTACTTGTTCACCCCTAATTCATATTTAGTTTCTTTTAAGTTGACATGGAATTTATCCGCAAATATGATAATTGTTGTACTTGGGCTTAAAATGTGTTCTTAATTGGATTATGGATGGTTCTCTTAACAAGCTTTAGTTGGCTGATAAACTTTCCATTGTTCATTAAAGGGCTTGGCAGAGGTGGTATATACTCGTAGAAGTGATGCATTTGCTGCTCAGAAGCGCTATAACAATGTGTTACTGGATGGGAAGCCATTGAAGATTGAAATTCTTGGTAACAAC

mRNA sequence

ATGGCTACGCCTGTGGATATCTCACGAGAGGCTGTGATAAAGAAGAATAACCGTAAGAAGCTCAGAGCACGACGTAGGGCCCGTTGTGGGCGTGGAGCAGGTGGGTCTTTTAATGGTGGAAGAGTAGTGATAGGGTCGGTTCGTAATCTCAACATAAATGCACGACCATCTGCTTGCTCAATTAGCTTTAAACTAATGAAGAATGTTCAATGGCAGCATGATTTACTTGAAGACAGTCTTAGAGCTTCAGGGATTTCTGGAATTGAAATTGGCACAAAGTTGTACGTTTCCGACTTGAATTTTGGGGTGACCAAAGAAGATATAATGGAGTTGTTCTCTGCTGTTGGAGACCTGAAAAGATTTGCAATTCATTATGACAAAAATGGCCGTCCATGTGGCTTGGCAGAGGTGGTATATACTCGTAGAAGTGATGCATTTGCTGCTCAGAAGCGCTATAACAATGTGTTACTGGATGGGAAGCCATTGAAGATTGAAATTCTTGGTAACAAC

Coding sequence (CDS)

ATGGCTACGCCTGTGGATATCTCACGAGAGGCTGTGATAAAGAAGAATAACCGTAAGAAGCTCAGAGCACGACGTAGGGCCCGTTGTGGGCGTGGAGCAGGTGGGTCTTTTAATGGTGGAAGAGTAGTGATAGGGTCGGTTCGTAATCTCAACATAAATGCACGACCATCTGCTTGCTCAATTAGCTTTAAACTAATGAAGAATGTTCAATGGCAGCATGATTTACTTGAAGACAGTCTTAGAGCTTCAGGGATTTCTGGAATTGAAATTGGCACAAAGTTGTACGTTTCCGACTTGAATTTTGGGGTGACCAAAGAAGATATAATGGAGTTGTTCTCTGCTGTTGGAGACCTGAAAAGATTTGCAATTCATTATGACAAAAATGGCCGTCCATGTGGCTTGGCAGAGGTGGTATATACTCGTAGAAGTGATGCATTTGCTGCTCAGAAGCGCTATAACAATGTGTTACTGGATGGGAAGCCATTGAAGATTGAAATTCTTGGTAACAAC

Protein sequence

MATPVDISREAVIKKNNRKKLRARRRARCGRGAGGSFNGGRVVIGSVRNLNINARPSACSISFKLMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELFSAVGDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN

Homology

[Close]

BLAST of MS019167 vs. NCBI nr
Match: XP_022143315.1 (THO complex subunit 4D-like [Momordica charantia])

HSP 1 Score: 332.4 bits (851), Expect = 2.3e-87
Identity = 170/178 (95.51%), Postives = 170/178 (95.51%), Query Frame = 0


Query: 1   MATPVDISREAVIKKNNRKKLRARRRARCGRGAGGSFNGGRVVIGSVRNLNINARPSACS 60
           MATPVDISREAVIKKNNRKKLRARRRARCGRGAGGSFNGGRVVIGSVRNLNINARPSACS
Sbjct: 1   MATPVDISREAVIKKNNRKKLRARRRARCGRGAGGSFNGGRVVIGSVRNLNINARPSACS 60

Query: 61  ISFKL--------MKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELF 120
           ISFKL        MKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELF
Sbjct: 61  ISFKLSIFNPPRRMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELF 120

Query: 121 SAVGDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 171
           SAVGDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN
Sbjct: 121 SAVGDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 178

[Close]

BLAST of MS019167 vs. NCBI nr
Match: XP_022143379.1 (THO complex subunit 4D-like [Momordica charantia])

HSP 1 Score: 272.3 bits (695), Expect = 2.8e-69
Identity = 142/174 (81.61%), Postives = 155/174 (89.08%), Query Frame = 0


Query: 1   MATPVDISREAVIKKNNRKKLRARRRARCGRGAGGSFNGGRVVIGSVRN--LNINARPSA 60
           MATP+D+S E +IKKNNR+KLRAR RAR GRGAGGSFNGGRVVIGS+R   L+IN RPSA
Sbjct: 1   MATPLDMSLEDMIKKNNREKLRARGRARRGRGAGGSFNGGRVVIGSIRRGPLSINTRPSA 60

Query: 61  CSISF--KLMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELFSAVG 120
            SIS   + MKNVQWQHDL EDSLRASGISGIEIGTKLYVS+L++GVTKEDI ELFS +G
Sbjct: 61  FSISKPPRRMKNVQWQHDLFEDSLRASGISGIEIGTKLYVSNLDYGVTKEDIRELFSEIG 120

Query: 121 DLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 171
           DLKRFAIHYDKNGRP G AEVVYTRRSDAFAA KRYNNVLLDGKP+KIEILG+N
Sbjct: 121 DLKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEILGDN 174

[Close]

BLAST of MS019167 vs. NCBI nr
Match: XP_022935328.1 (THO complex subunit 4D-like [Cucurbita moschata] >XP_022935329.1 THO complex subunit 4D-like [Cucurbita moschata] >XP_022935330.1 THO complex subunit 4D-like [Cucurbita moschata])

HSP 1 Score: 268.1 bits (684), Expect = 5.3e-68
Identity = 143/175 (81.71%), Postives = 155/175 (88.57%), Query Frame = 0


Query: 1   MATPVDISREAVIKKNNRKKLRARRRARCGRGAGGSFNGGR-VVIGSVRN--LNINARPS 60
           MATP+D+S E VIKKNNR+KLRAR RAR GRGAGGS+NGGR VVIGSVR   L INARPS
Sbjct: 1   MATPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSYNGGRAVVIGSVRRGPLGINARPS 60

Query: 61  ACSISF--KLMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELFSAV 120
           A SIS   + MKNVQWQHDL EDSLRASGISGIEIGTKLYVS+L++GVTKEDI ELFS +
Sbjct: 61  AFSISKPPRRMKNVQWQHDLFEDSLRASGISGIEIGTKLYVSNLDYGVTKEDIRELFSEI 120

Query: 121 GDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 171
           GDLKRFAIHYDKNGRP G AEVVYTRRSDAFAA KRYNNVLLDGKP+KIE+LG+N
Sbjct: 121 GDLKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDN 175

[Close]

BLAST of MS019167 vs. NCBI nr
Match: XP_023527122.1 (THO complex subunit 4D [Cucurbita pepo subsp. pepo])

HSP 1 Score: 268.1 bits (684), Expect = 5.3e-68
Identity = 143/175 (81.71%), Postives = 155/175 (88.57%), Query Frame = 0


Query: 1   MATPVDISREAVIKKNNRKKLRARRRARCGRGAGGSFNGGR-VVIGSVRN--LNINARPS 60
           MATP+D+S E VIKKNNR+KLRAR RAR GRGAGGS+NGGR VVIGSVR   L INARPS
Sbjct: 1   MATPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSYNGGRAVVIGSVRRGPLGINARPS 60

Query: 61  ACSISF--KLMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELFSAV 120
           A SIS   + MKNVQWQHDL EDSLRASGISGIEIGTKLYVS+L++GVTKEDI ELFS +
Sbjct: 61  AFSISKPPRRMKNVQWQHDLFEDSLRASGISGIEIGTKLYVSNLDYGVTKEDIRELFSEI 120

Query: 121 GDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 171
           GDLKRFAIHYDKNGRP G AEVVYTRRSDAFAA KRYNNVLLDGKP+KIE+LG+N
Sbjct: 121 GDLKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDN 175

[Close]

BLAST of MS019167 vs. NCBI nr
Match: XP_022982649.1 (THO complex subunit 4D-like [Cucurbita maxima] >XP_022982650.1 THO complex subunit 4D-like [Cucurbita maxima] >XP_022982651.1 THO complex subunit 4D-like [Cucurbita maxima])

HSP 1 Score: 268.1 bits (684), Expect = 5.3e-68
Identity = 143/175 (81.71%), Postives = 155/175 (88.57%), Query Frame = 0


Query: 1   MATPVDISREAVIKKNNRKKLRARRRARCGRGAGGSFNGGR-VVIGSVRN--LNINARPS 60
           MATP+D+S E VIKKNNR+KLRAR RAR GRGAGGS+NGGR VVIGSVR   L INARPS
Sbjct: 1   MATPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSYNGGRAVVIGSVRRGPLGINARPS 60

Query: 61  ACSISF--KLMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELFSAV 120
           A SIS   + MKNVQWQHDL EDSLRASGISGIEIGTKLYVS+L++GVTKEDI ELFS +
Sbjct: 61  AFSISKPPRRMKNVQWQHDLFEDSLRASGISGIEIGTKLYVSNLDYGVTKEDIRELFSEI 120

Query: 121 GDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 171
           GDLKRFAIHYDKNGRP G AEVVYTRRSDAFAA KRYNNVLLDGKP+KIE+LG+N
Sbjct: 121 GDLKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDN 175

[Close]

BLAST of MS019167 vs. ExPASy Swiss-Prot
Match: Q6NQ72 (THO complex subunit 4D OS=Arabidopsis thaliana OX=3702 GN=ALY4 PE=1 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 1.1e-39
Identity = 87/174 (50.00%), Postives = 126/174 (72.41%), Query Frame = 0


Query: 1   MATPVDISREAVIKKNNRKKLRARRRAR-CGRGAGGSFNGGRVVIGSVRN-LNINARPSA 60
           M+  ++++ + ++K+    +   R  +R  GRG GG   GGR    + R  L +NARPS+
Sbjct: 1   MSGALNMTLDEIVKRGKTARSGGRGISRGRGRGRGG---GGRGAGPARRGPLAVNARPSS 60

Query: 61  CSIS--FKLMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELFSAVG 120
            +I+   + ++++ WQ  L ED LRA+G SG+E+GT+L+V++L+ GVT EDI ELFS +G
Sbjct: 61  FTINKPVRRVRSLPWQSGLFEDGLRAAGASGVEVGTRLHVTNLDQGVTNEDIRELFSEIG 120

Query: 121 DLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 171
           +++R+AIHYDKNGRP G AEVVY RRSDAF A K+YNNVLLDG+P+++EILG N
Sbjct: 121 EVERYAIHYDKNGRPSGTAEVVYPRRSDAFQALKKYNNVLLDGRPMRLEILGGN 171

[Close]

BLAST of MS019167 vs. ExPASy Swiss-Prot
Match: Q94EH8 (THO complex subunit 4C OS=Arabidopsis thaliana OX=3702 GN=ALY3 PE=1 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 7.7e-38
Identity = 87/185 (47.03%), Postives = 125/185 (67.57%), Query Frame = 0


Query: 1   MATPVDISREAVIKKNNRKKLRARRRA------RCGRGAGG--SFNGGRVVIGSVRN--L 60
           M+  ++++ + ++KK+  ++  A R        + GRG GG     GG    G VR   L
Sbjct: 1   MSDALNMTLDEIVKKSKSERSAAARSGGKGVSRKSGRGRGGPNGVVGGGRGGGPVRRGPL 60

Query: 61  NINARPSACSISFKL---MKNVQW--QHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTK 120
            +N RPS+     KL    +++ W  Q+DL E++LRA G+SG+E+GT +Y+++L+ GVT 
Sbjct: 61  AVNTRPSSSFSINKLARRKRSLPWQNQNDLYEETLRAVGVSGVEVGTTVYITNLDQGVTN 120

Query: 121 EDIMELFSAVGDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIE 171
           EDI EL++ +G+LKR+AIHYDKNGRP G AEVVY RRSDA  A ++YNNVLLDG+P+K+E
Sbjct: 121 EDIRELYAEIGELKRYAIHYDKNGRPSGSAEVVYMRRSDAIQAMRKYNNVLLDGRPMKLE 180

[Close]

BLAST of MS019167 vs. ExPASy Swiss-Prot
Match: Q8L719 (THO complex subunit 4B OS=Arabidopsis thaliana OX=3702 GN=ALY2 PE=1 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 1.2e-30
Identity = 84/190 (44.21%), Postives = 113/190 (59.47%), Query Frame = 0


Query: 1   MATPVDISREAVIKKNNRKKLRARRRARCGRGAGGSFNGGRVVIGS--------VRNLNI 60
           M+  +D+S + +I K+NRK   +R R   G   GG+  GGR   GS           +  
Sbjct: 1   MSGGLDMSLDDII-KSNRKPTGSRGRGGIG---GGNNTGGRGGSGSNSGPSRRFANRVGA 60

Query: 61  NARPSACSISFKLMKNVQWQHDLL--EDSLRAS----------GISGIEIGTKLYVSDLN 120
              P +  I  +   +  WQ+D+   + S+ A+          G S IE GTKLY+S+L+
Sbjct: 61  RTAPYSRPIQQQQAHDAMWQNDVFATDASVAAAFGHHQTAVVGGGSSIETGTKLYISNLD 120

Query: 121 FGVTKEDIMELFSAVGDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGK 171
           +GV+ EDI ELFS VGDLKR+ IHYD++GR  G AEVV++RR DA AA KRYNNV LDGK
Sbjct: 121 YGVSNEDIKELFSEVGDLKRYGIHYDRSGRSKGTAEVVFSRRGDALAAVKRYNNVQLDGK 180

[Close]

BLAST of MS019167 vs. ExPASy Swiss-Prot
Match: Q8L773 (THO complex subunit 4A OS=Arabidopsis thaliana OX=3702 GN=ALY1 PE=1 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 2.0e-30
Identity = 78/176 (44.32%), Postives = 107/176 (60.80%), Query Frame = 0


Query: 1   MATPVDISREAVIKKNNRKKLRARRRARCGRGAGGSFNGGRVVIGSVRNLNINARPSACS 60
           M+T +D+S + +I KN + +  A      G G+G          G  R  N N R S  S
Sbjct: 1   MSTGLDMSLDDMIAKNRKSRGGAGPARGTGSGSGP---------GPTRRNNPN-RKSTRS 60

Query: 61  ISFKLMKNVQ--WQHDLL----EDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELFSA 120
             ++  K  +  W HD+     ED       +GIE GTKLY+S+L++GV  EDI ELF+ 
Sbjct: 61  APYQSAKAPESTWGHDMFSDRSEDHRSGRSSAGIETGTKLYISNLDYGVMNEDIKELFAE 120

Query: 121 VGDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 171
           VG+LKR+ +H+D++GR  G AEVVY+RR DA AA K+YN+V LDGKP+KIEI+G N
Sbjct: 121 VGELKRYTVHFDRSGRSKGTAEVVYSRRGDALAAVKKYNDVQLDGKPMKIEIVGTN 166

[Close]

BLAST of MS019167 vs. ExPASy Swiss-Prot
Match: B5FXN8 (THO complex subunit 4 OS=Taeniopygia guttata OX=59729 GN=ALYREF PE=2 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 1.8e-26
Identity = 75/183 (40.98%), Postives = 107/183 (58.47%), Query Frame = 0


Query: 1   MATPVDISREAVIKKNNRKKLRARRRARCGRGAGGS----------FNGGRVVIGSVRNL 60
           MA  +D+S + +IK  NR +  A R  R GRG GG+            GGR   G VRN 
Sbjct: 1   MADKMDMSLDDIIKL-NRSQRGASRGGRGGRGRGGTARGGGPGRGGVGGGRAGGGPVRNR 60

Query: 61  NINA------RPSACSISFKLMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVT 120
            + A      RP+  S   +L +  +WQHDL +    A   +G+E G KL VS+L+FGV+
Sbjct: 61  PVMARGGGRNRPAPYSRPKQLPE--KWQHDLFDSGFGAG--AGVETGGKLLVSNLDFGVS 120

Query: 121 KEDIMELFSAVGDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKI 168
             DI ELF+  G LK+ A+HYD++GR  G A+V + R++DA  A K+YN V LDG+P+ I
Sbjct: 121 DADIQELFAEFGTLKKAAVHYDRSGRSLGTADVHFERKADALKAMKQYNGVPLDGRPMNI 178

[Close]

BLAST of MS019167 vs. ExPASy TrEMBL
Match: A0A6J1CNH0 (THO complex subunit 4D-like OS=Momordica charantia OX=3673 GN=LOC111013215 PE=4 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 1.1e-87
Identity = 170/178 (95.51%), Postives = 170/178 (95.51%), Query Frame = 0


Query: 1   MATPVDISREAVIKKNNRKKLRARRRARCGRGAGGSFNGGRVVIGSVRNLNINARPSACS 60
           MATPVDISREAVIKKNNRKKLRARRRARCGRGAGGSFNGGRVVIGSVRNLNINARPSACS
Sbjct: 1   MATPVDISREAVIKKNNRKKLRARRRARCGRGAGGSFNGGRVVIGSVRNLNINARPSACS 60

Query: 61  ISFKL--------MKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELF 120
           ISFKL        MKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELF
Sbjct: 61  ISFKLSIFNPPRRMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELF 120

Query: 121 SAVGDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 171
           SAVGDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN
Sbjct: 121 SAVGDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 178

[Close]

BLAST of MS019167 vs. ExPASy TrEMBL
Match: A0A6J1CP51 (THO complex subunit 4D-like OS=Momordica charantia OX=3673 GN=LOC111013262 PE=4 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 1.3e-69
Identity = 142/174 (81.61%), Postives = 155/174 (89.08%), Query Frame = 0


Query: 1   MATPVDISREAVIKKNNRKKLRARRRARCGRGAGGSFNGGRVVIGSVRN--LNINARPSA 60
           MATP+D+S E +IKKNNR+KLRAR RAR GRGAGGSFNGGRVVIGS+R   L+IN RPSA
Sbjct: 1   MATPLDMSLEDMIKKNNREKLRARGRARRGRGAGGSFNGGRVVIGSIRRGPLSINTRPSA 60

Query: 61  CSISF--KLMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELFSAVG 120
            SIS   + MKNVQWQHDL EDSLRASGISGIEIGTKLYVS+L++GVTKEDI ELFS +G
Sbjct: 61  FSISKPPRRMKNVQWQHDLFEDSLRASGISGIEIGTKLYVSNLDYGVTKEDIRELFSEIG 120

Query: 121 DLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 171
           DLKRFAIHYDKNGRP G AEVVYTRRSDAFAA KRYNNVLLDGKP+KIEILG+N
Sbjct: 121 DLKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEILGDN 174

[Close]

BLAST of MS019167 vs. ExPASy TrEMBL
Match: A0A6J1J564 (THO complex subunit 4D-like OS=Cucurbita maxima OX=3661 GN=LOC111481464 PE=4 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 2.5e-68
Identity = 143/175 (81.71%), Postives = 155/175 (88.57%), Query Frame = 0


Query: 1   MATPVDISREAVIKKNNRKKLRARRRARCGRGAGGSFNGGR-VVIGSVRN--LNINARPS 60
           MATP+D+S E VIKKNNR+KLRAR RAR GRGAGGS+NGGR VVIGSVR   L INARPS
Sbjct: 1   MATPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSYNGGRAVVIGSVRRGPLGINARPS 60

Query: 61  ACSISF--KLMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELFSAV 120
           A SIS   + MKNVQWQHDL EDSLRASGISGIEIGTKLYVS+L++GVTKEDI ELFS +
Sbjct: 61  AFSISKPPRRMKNVQWQHDLFEDSLRASGISGIEIGTKLYVSNLDYGVTKEDIRELFSEI 120

Query: 121 GDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 171
           GDLKRFAIHYDKNGRP G AEVVYTRRSDAFAA KRYNNVLLDGKP+KIE+LG+N
Sbjct: 121 GDLKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDN 175

[Close]

BLAST of MS019167 vs. ExPASy TrEMBL
Match: A0A6J1FAB9 (THO complex subunit 4D-like OS=Cucurbita moschata OX=3662 GN=LOC111442248 PE=4 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 2.5e-68
Identity = 143/175 (81.71%), Postives = 155/175 (88.57%), Query Frame = 0


Query: 1   MATPVDISREAVIKKNNRKKLRARRRARCGRGAGGSFNGGR-VVIGSVRN--LNINARPS 60
           MATP+D+S E VIKKNNR+KLRAR RAR GRGAGGS+NGGR VVIGSVR   L INARPS
Sbjct: 1   MATPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSYNGGRAVVIGSVRRGPLGINARPS 60

Query: 61  ACSISF--KLMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELFSAV 120
           A SIS   + MKNVQWQHDL EDSLRASGISGIEIGTKLYVS+L++GVTKEDI ELFS +
Sbjct: 61  AFSISKPPRRMKNVQWQHDLFEDSLRASGISGIEIGTKLYVSNLDYGVTKEDIRELFSEI 120

Query: 121 GDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 171
           GDLKRFAIHYDKNGRP G AEVVYTRRSDAFAA KRYNNVLLDGKP+KIE+LG+N
Sbjct: 121 GDLKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDN 175

[Close]

BLAST of MS019167 vs. ExPASy TrEMBL
Match: A0A1S3C452 (THO complex subunit 4D OS=Cucumis melo OX=3656 GN=LOC103496802 PE=4 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 5.3e-66
Identity = 140/175 (80.00%), Postives = 151/175 (86.29%), Query Frame = 0


Query: 1   MATPVDISREAVIKKNNRKKLRARRRARCGRGAGGSFNGGR-VVIGSVRN--LNINARPS 60
           M TP+D+S E VIKKNNR+KLRAR RAR GRGAGGSFNGGR VVIGSVR   L INAR S
Sbjct: 1   MTTPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSFNGGRGVVIGSVRRGPLGINARAS 60

Query: 61  ACSISF--KLMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELFSAV 120
           A SI      MKNVQWQHDL EDSLRASGISGI+IGTKLYVS+L++GVTKEDI ELFS +
Sbjct: 61  AYSIRKPPHRMKNVQWQHDLFEDSLRASGISGIQIGTKLYVSNLDYGVTKEDIRELFSEI 120

Query: 121 GDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 171
           GDLKRFAIHYDKNGRP G AEVVYTRRSDAFAA KRYNNVLLDGKP+KIE+LG+N
Sbjct: 121 GDLKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDN 175

[Close]

BLAST of MS019167 vs. TAIR 10
Match: AT5G37720.1 (ALWAYS EARLY 4 )

HSP 1 Score: 164.5 bits (415), Expect = 7.6e-41
Identity = 87/174 (50.00%), Postives = 126/174 (72.41%), Query Frame = 0


Query: 1   MATPVDISREAVIKKNNRKKLRARRRAR-CGRGAGGSFNGGRVVIGSVRN-LNINARPSA 60
           M+  ++++ + ++K+    +   R  +R  GRG GG   GGR    + R  L +NARPS+
Sbjct: 1   MSGALNMTLDEIVKRGKTARSGGRGISRGRGRGRGG---GGRGAGPARRGPLAVNARPSS 60

Query: 61  CSIS--FKLMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELFSAVG 120
            +I+   + ++++ WQ  L ED LRA+G SG+E+GT+L+V++L+ GVT EDI ELFS +G
Sbjct: 61  FTINKPVRRVRSLPWQSGLFEDGLRAAGASGVEVGTRLHVTNLDQGVTNEDIRELFSEIG 120

Query: 121 DLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 171
           +++R+AIHYDKNGRP G AEVVY RRSDAF A K+YNNVLLDG+P+++EILG N
Sbjct: 121 EVERYAIHYDKNGRPSGTAEVVYPRRSDAFQALKKYNNVLLDGRPMRLEILGGN 171

[Close]

BLAST of MS019167 vs. TAIR 10
Match: AT5G37720.2 (ALWAYS EARLY 4 )

HSP 1 Score: 164.5 bits (415), Expect = 7.6e-41
Identity = 87/174 (50.00%), Postives = 126/174 (72.41%), Query Frame = 0


Query: 1   MATPVDISREAVIKKNNRKKLRARRRAR-CGRGAGGSFNGGRVVIGSVRN-LNINARPSA 60
           M+  ++++ + ++K+    +   R  +R  GRG GG   GGR    + R  L +NARPS+
Sbjct: 1   MSGALNMTLDEIVKRGKTARSGGRGISRGRGRGRGG---GGRGAGPARRGPLAVNARPSS 60

Query: 61  CSIS--FKLMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELFSAVG 120
            +I+   + ++++ WQ  L ED LRA+G SG+E+GT+L+V++L+ GVT EDI ELFS +G
Sbjct: 61  FTINKPVRRVRSLPWQSGLFEDGLRAAGASGVEVGTRLHVTNLDQGVTNEDIRELFSEIG 120

Query: 121 DLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 171
           +++R+AIHYDKNGRP G AEVVY RRSDAF A K+YNNVLLDG+P+++EILG N
Sbjct: 121 EVERYAIHYDKNGRPSGTAEVVYPRRSDAFQALKKYNNVLLDGRPMRLEILGGN 171

[Close]

BLAST of MS019167 vs. TAIR 10
Match: AT1G66260.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 158.3 bits (399), Expect = 5.5e-39
Identity = 87/185 (47.03%), Postives = 125/185 (67.57%), Query Frame = 0


Query: 1   MATPVDISREAVIKKNNRKKLRARRRA------RCGRGAGG--SFNGGRVVIGSVRN--L 60
           M+  ++++ + ++KK+  ++  A R        + GRG GG     GG    G VR   L
Sbjct: 1   MSDALNMTLDEIVKKSKSERSAAARSGGKGVSRKSGRGRGGPNGVVGGGRGGGPVRRGPL 60

Query: 61  NINARPSACSISFKL---MKNVQW--QHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTK 120
            +N RPS+     KL    +++ W  Q+DL E++LRA G+SG+E+GT +Y+++L+ GVT 
Sbjct: 61  AVNTRPSSSFSINKLARRKRSLPWQNQNDLYEETLRAVGVSGVEVGTTVYITNLDQGVTN 120

Query: 121 EDIMELFSAVGDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIE 171
           EDI EL++ +G+LKR+AIHYDKNGRP G AEVVY RRSDA  A ++YNNVLLDG+P+K+E
Sbjct: 121 EDIRELYAEIGELKRYAIHYDKNGRPSGSAEVVYMRRSDAIQAMRKYNNVLLDGRPMKLE 180

[Close]

BLAST of MS019167 vs. TAIR 10
Match: AT1G66260.2 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 158.3 bits (399), Expect = 5.5e-39
Identity = 87/185 (47.03%), Postives = 125/185 (67.57%), Query Frame = 0


Query: 1   MATPVDISREAVIKKNNRKKLRARRRA------RCGRGAGG--SFNGGRVVIGSVRN--L 60
           M+  ++++ + ++KK+  ++  A R        + GRG GG     GG    G VR   L
Sbjct: 1   MSDALNMTLDEIVKKSKSERSAAARSGGKGVSRKSGRGRGGPNGVVGGGRGGGPVRRGPL 60

Query: 61  NINARPSACSISFKL---MKNVQW--QHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTK 120
            +N RPS+     KL    +++ W  Q+DL E++LRA G+SG+E+GT +Y+++L+ GVT 
Sbjct: 61  AVNTRPSSSFSINKLARRKRSLPWQNQNDLYEETLRAVGVSGVEVGTTVYITNLDQGVTN 120

Query: 121 EDIMELFSAVGDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIE 171
           EDI EL++ +G+LKR+AIHYDKNGRP G AEVVY RRSDA  A ++YNNVLLDG+P+K+E
Sbjct: 121 EDIRELYAEIGELKRYAIHYDKNGRPSGSAEVVYMRRSDAIQAMRKYNNVLLDGRPMKLE 180

[Close]

BLAST of MS019167 vs. TAIR 10
Match: AT5G59950.3 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 136.0 bits (341), Expect = 2.9e-32
Identity = 77/174 (44.25%), Postives = 105/174 (60.34%), Query Frame = 0


Query: 1   MATPVDISREAVIKKNNRKKLRARRRARCGRGAGGSFNGGRVVIGSVRNLNINARPSACS 60
           M+T +D+S + +I KN + +  A      G G+G          G  R  N N R S  S
Sbjct: 1   MSTGLDMSLDDMIAKNRKSRGGAGPARGTGSGSGP---------GPTRRNNPN-RKSTRS 60

Query: 61  ISFKLMKNVQWQHDLL----EDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELFSAVG 120
             ++      W HD+     ED       +GIE GTKLY+S+L++GV  EDI ELF+ VG
Sbjct: 61  APYQSAPESTWGHDMFSDRSEDHRSGRSSAGIETGTKLYISNLDYGVMNEDIKELFAEVG 120

Query: 121 DLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 171
           +LKR+ +H+D++GR  G AEVVY+RR DA AA K+YN+V LDGKP+KIEI+G N
Sbjct: 121 ELKRYTVHFDRSGRSKGTAEVVYSRRGDALAAVKKYNDVQLDGKPMKIEIVGTN 164

The following BLAST results are available for this feature:

BLAST of MS019167 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143315.1	2.3e-87	95.51	THO complex subunit 4D-like [Momordica charantia]	[more]
XP_022143379.1	2.8e-69	81.61	THO complex subunit 4D-like [Momordica charantia]	[more]
XP_022935328.1	5.3e-68	81.71	THO complex subunit 4D-like [Cucurbita moschata] >XP_022935329.1 THO complex sub...	[more]
XP_023527122.1	5.3e-68	81.71	THO complex subunit 4D [Cucurbita pepo subsp. pepo]	[more]
XP_022982649.1	5.3e-68	81.71	THO complex subunit 4D-like [Cucurbita maxima] >XP_022982650.1 THO complex subun...	[more]

BLAST of MS019167 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NQ72	1.1e-39	50.00	THO complex subunit 4D OS=Arabidopsis thaliana OX=3702 GN=ALY4 PE=1 SV=1	[more]
Q94EH8	7.7e-38	47.03	THO complex subunit 4C OS=Arabidopsis thaliana OX=3702 GN=ALY3 PE=1 SV=1	[more]
Q8L719	1.2e-30	44.21	THO complex subunit 4B OS=Arabidopsis thaliana OX=3702 GN=ALY2 PE=1 SV=1	[more]
Q8L773	2.0e-30	44.32	THO complex subunit 4A OS=Arabidopsis thaliana OX=3702 GN=ALY1 PE=1 SV=1	[more]
B5FXN8	1.8e-26	40.98	THO complex subunit 4 OS=Taeniopygia guttata OX=59729 GN=ALYREF PE=2 SV=1	[more]

BLAST of MS019167 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CNH0	1.1e-87	95.51	THO complex subunit 4D-like OS=Momordica charantia OX=3673 GN=LOC111013215 PE=4 ...	[more]
A0A6J1CP51	1.3e-69	81.61	THO complex subunit 4D-like OS=Momordica charantia OX=3673 GN=LOC111013262 PE=4 ...	[more]
A0A6J1J564	2.5e-68	81.71	THO complex subunit 4D-like OS=Cucurbita maxima OX=3661 GN=LOC111481464 PE=4 SV=...	[more]
A0A6J1FAB9	2.5e-68	81.71	THO complex subunit 4D-like OS=Cucurbita moschata OX=3662 GN=LOC111442248 PE=4 S...	[more]
A0A1S3C452	5.3e-66	80.00	THO complex subunit 4D OS=Cucumis melo OX=3656 GN=LOC103496802 PE=4 SV=1	[more]

BLAST of MS019167 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G37720.1	7.6e-41	50.00	ALWAYS EARLY 4	[more]
AT5G37720.2	7.6e-41	50.00	ALWAYS EARLY 4	[more]
AT1G66260.1	5.5e-39	47.03	RNA-binding (RRM/RBD/RNP motifs) family protein	[more]
AT1G66260.2	5.5e-39	47.03	RNA-binding (RRM/RBD/RNP motifs) family protein	[more]
AT5G59950.3	2.9e-32	44.25	RNA-binding (RRM/RBD/RNP motifs) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000504	RNA recognition motif domain	SMART	SM00360	rrm1_1	coord: 93..165 e-value: 3.8E-16 score: 69.6
IPR000504	RNA recognition motif domain	PFAM	PF00076	RRM_1	coord: 94..163 e-value: 3.4E-11 score: 42.8
IPR000504	RNA recognition motif domain	PROSITE	PS50102	RRM	coord: 92..169 score: 13.532493
IPR012677	Nucleotide-binding alpha-beta plait domain superfamily	GENE3D	3.30.70.330		coord: 89..168 e-value: 1.5E-18 score: 68.8
None	No IPR available	PANTHER	PTHR19965	RNA AND EXPORT FACTOR BINDING PROTEIN	coord: 1..170
None	No IPR available	PANTHER	PTHR19965:SF33	THO COMPLEX SUBUNIT 4C	coord: 1..170
IPR035979	RNA-binding domain superfamily	SUPERFAMILY	54928	RNA-binding domain, RBD	coord: 86..166

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS019167.1	MS019167.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding