MS019154 (gene) Bitter gourd (TR) v1

BLAST of MS019154 vs. NCBI nr
Match: XP_022143444.1 (U-box domain-containing protein 21 isoform X1 [Momordica charantia])

HSP 1 Score: 863.2 bits (2229), Expect = 9.6e-247
Identity = 440/442 (99.55%), Postives = 441/442 (99.77%), Query Frame = 0

Query: 1   MILSWRKNKLGLLSKLKKEVPGVDSGAEIEIPSHFLCPISLDLMKDPVILCTGITYDRES 60
           MILSWRKNKLGLLSKLKKEVPGVDSGAEIEIPSHFLCPISLDLMKDPVILCTGITYDRES
Sbjct: 1   MILSWRKNKLGLLSKLKKEVPGVDSGAEIEIPSHFLCPISLDLMKDPVILCTGITYDRES 60

Query: 61  IEKWIDAGNFLCPVTKQGLTGFDLIPNHALRRMIQDWCVANRSYGIERIPTPRIPVTPYE 120
           IEKWIDAGNFLCPVTKQGLTGFDLIPNHALRRMIQDWCVANRSYGIERIPTPRIPVTPYE
Sbjct: 61  IEKWIDAGNFLCPVTKQGLTGFDLIPNHALRRMIQDWCVANRSYGIERIPTPRIPVTPYE 120

Query: 121 VSEICSRIAAATQRTDSKRCGELVGKTRNWGRESERNRRCIVSGGTGNVLAASFEHFAGV 180
           VSEICSRIAAATQRTDSKRCGELVGK RNWGRESERNRRCIVSGGTGNVLAASFEHFAGV
Sbjct: 121 VSEICSRIAAATQRTDSKRCGELVGKIRNWGRESERNRRCIVSGGTGNVLAASFEHFAGV 180

Query: 181 SIEKHAGLLEEILLVLVWMFPIAMEGLSKLGSADSLKCLVSFLVGKDLSPKKSAVFVLKE 240
           SIEKHAGLLEEILLVLVWMFPIAMEGLSKLGSADSLKCLVSFLVGKDLSPKKSAVFVLKE
Sbjct: 181 SIEKHAGLLEEILLVLVWMFPIAMEGLSKLGSADSLKCLVSFLVGKDLSPKKSAVFVLKE 240

Query: 241 LLLVDQRFVDSLAAIEGVPEALVSIVKEPLCSSATRSSLTAIFYMILPSEISEKMALKFV 300
           LLLVDQRFVDSLAAIEGVPEALVSIVKEPLCSSATRSSLTAIFYMILPSEISEKMALKF+
Sbjct: 241 LLLVDQRFVDSLAAIEGVPEALVSIVKEPLCSSATRSSLTAIFYMILPSEISEKMALKFM 300

Query: 301 ELGLVSLLLEFLVDAEKSLCEKALGILDGICDFKQGREKLYQNALTIPLLVKKILRVSEL 360
           ELGLVSLLLEFLVDAEKSLCEKALGILDGICDFKQGREKLYQNALTIPLLVKKILRVSEL
Sbjct: 301 ELGLVSLLLEFLVDAEKSLCEKALGILDGICDFKQGREKLYQNALTIPLLVKKILRVSEL 360

Query: 361 STEFCLSILLKLCKSGERDEDDVRVEAAQLGAFQKILVLLQVGCGGAMKDKVTELLKLLN 420
           STEFCLSILLKLCKSGERDEDDVRVEAAQLGAFQKILVLLQVGCGGAMKDKVTELLKLLN
Sbjct: 361 STEFCLSILLKLCKSGERDEDDVRVEAAQLGAFQKILVLLQVGCGGAMKDKVTELLKLLN 420

Query: 421 LYKDRVDCIDSSMHFKYLKKSF 443
           LYKDRVDCIDSSMHFKYLKKSF
Sbjct: 421 LYKDRVDCIDSSMHFKYLKKSF 442

BLAST of MS019154 vs. NCBI nr
Match: XP_022143445.1 (U-box domain-containing protein 21 isoform X2 [Momordica charantia])

HSP 1 Score: 803.5 bits (2074), Expect = 9.0e-229
Identity = 410/412 (99.51%), Postives = 411/412 (99.76%), Query Frame = 0

Query: 1   MILSWRKNKLGLLSKLKKEVPGVDSGAEIEIPSHFLCPISLDLMKDPVILCTGITYDRES 60
           MILSWRKNKLGLLSKLKKEVPGVDSGAEIEIPSHFLCPISLDLMKDPVILCTGITYDRES
Sbjct: 1   MILSWRKNKLGLLSKLKKEVPGVDSGAEIEIPSHFLCPISLDLMKDPVILCTGITYDRES 60

Query: 61  IEKWIDAGNFLCPVTKQGLTGFDLIPNHALRRMIQDWCVANRSYGIERIPTPRIPVTPYE 120
           IEKWIDAGNFLCPVTKQGLTGFDLIPNHALRRMIQDWCVANRSYGIERIPTPRIPVTPYE
Sbjct: 61  IEKWIDAGNFLCPVTKQGLTGFDLIPNHALRRMIQDWCVANRSYGIERIPTPRIPVTPYE 120

Query: 121 VSEICSRIAAATQRTDSKRCGELVGKTRNWGRESERNRRCIVSGGTGNVLAASFEHFAGV 180
           VSEICSRIAAATQRTDSKRCGELVGK RNWGRESERNRRCIVSGGTGNVLAASFEHFAGV
Sbjct: 121 VSEICSRIAAATQRTDSKRCGELVGKIRNWGRESERNRRCIVSGGTGNVLAASFEHFAGV 180

Query: 181 SIEKHAGLLEEILLVLVWMFPIAMEGLSKLGSADSLKCLVSFLVGKDLSPKKSAVFVLKE 240
           SIEKHAGLLEEILLVLVWMFPIAMEGLSKLGSADSLKCLVSFLVGKDLSPKKSAVFVLKE
Sbjct: 181 SIEKHAGLLEEILLVLVWMFPIAMEGLSKLGSADSLKCLVSFLVGKDLSPKKSAVFVLKE 240

Query: 241 LLLVDQRFVDSLAAIEGVPEALVSIVKEPLCSSATRSSLTAIFYMILPSEISEKMALKFV 300
           LLLVDQRFVDSLAAIEGVPEALVSIVKEPLCSSATRSSLTAIFYMILPSEISEKMALKF+
Sbjct: 241 LLLVDQRFVDSLAAIEGVPEALVSIVKEPLCSSATRSSLTAIFYMILPSEISEKMALKFM 300

Query: 301 ELGLVSLLLEFLVDAEKSLCEKALGILDGICDFKQGREKLYQNALTIPLLVKKILRVSEL 360
           ELGLVSLLLEFLVDAEKSLCEKALGILDGICDFKQGREKLYQNALTIPLLVKKILRVSEL
Sbjct: 301 ELGLVSLLLEFLVDAEKSLCEKALGILDGICDFKQGREKLYQNALTIPLLVKKILRVSEL 360

Query: 361 STEFCLSILLKLCKSGERDEDDVRVEAAQLGAFQKILVLLQVGCGGAMKDKV 413
           STEFCLSILLKLCKSGERDEDDVRVEAAQLGAFQKILVLLQVGCGGAMKDKV
Sbjct: 361 STEFCLSILLKLCKSGERDEDDVRVEAAQLGAFQKILVLLQVGCGGAMKDKV 412

BLAST of MS019154 vs. NCBI nr
Match: XP_004145841.1 (U-box domain-containing protein 21 [Cucumis sativus] >KGN57802.1 hypothetical protein Csa_009631 [Cucumis sativus])

HSP 1 Score: 763.5 bits (1970), Expect = 1.0e-216
Identity = 381/442 (86.20%), Postives = 416/442 (94.12%), Query Frame = 0

Query: 1   MILSWRKNKLGLLSKLKKEVPGVDSGAEIEIPSHFLCPISLDLMKDPVILCTGITYDRES 60
           MI SWRKNKLG L+KLKKE+PGVDSG+EI IPSH++CPISLDLMKDPVIL TGITYDRES
Sbjct: 1   MIFSWRKNKLGFLTKLKKELPGVDSGSEIAIPSHYMCPISLDLMKDPVILSTGITYDRES 60

Query: 61  IEKWIDAGNFLCPVTKQGLTGFDLIPNHALRRMIQDWCVANRSYGIERIPTPRIPVTPYE 120
           IEKWID GNF CPVTKQ LT FDLIPNHALRR+IQDWCVANRSYGIERIPTPRIPV+PYE
Sbjct: 61  IEKWIDGGNFSCPVTKQDLTVFDLIPNHALRRLIQDWCVANRSYGIERIPTPRIPVSPYE 120

Query: 121 VSEICSRIAAATQRTDSKRCGELVGKTRNWGRESERNRRCIVSGGTGNVLAASFEHFAGV 180
           V EICSRI+ ATQR+DSKRC EL+GK RNW +ESERNRRCIV+GGTG+VLAASFEHFAGV
Sbjct: 121 VKEICSRISIATQRSDSKRCSELMGKIRNWAKESERNRRCIVNGGTGDVLAASFEHFAGV 180

Query: 181 SIEKHAGLLEEILLVLVWMFPIAMEGLSKLGSADSLKCLVSFLVGKDLSPKKSAVFVLKE 240
           SIEKH GLLEEILLVL  ++P+A+EGLSKLGSADSLKCLVSFLVGKDLSPK+SA+FVLKE
Sbjct: 181 SIEKHVGLLEEILLVLTCVYPVAIEGLSKLGSADSLKCLVSFLVGKDLSPKQSAIFVLKE 240

Query: 241 LLLVDQRFVDSLAAIEGVPEALVSIVKEPLCSSATRSSLTAIFYMILPSEISEKMALKFV 300
           LL  D+R+V+SLAAIEGV EALVSI+++PLC SAT+SSLTAIFYMILPS+I EKMALKFV
Sbjct: 241 LLAADRRYVNSLAAIEGVSEALVSIIRDPLCPSATKSSLTAIFYMILPSDIGEKMALKFV 300

Query: 301 ELGLVSLLLEFLVDAEKSLCEKALGILDGICDFKQGREKLYQNALTIPLLVKKILRVSEL 360
           ELGLVS LLEFLVDAEKSLCEKALGILDGICD+KQGREKLY NALTIPLLVKKILRVSEL
Sbjct: 301 ELGLVSQLLEFLVDAEKSLCEKALGILDGICDYKQGREKLYNNALTIPLLVKKILRVSEL 360

Query: 361 STEFCLSILLKLCKSGERDEDDVRVEAAQLGAFQKILVLLQVGCGGAMKDKVTELLKLLN 420
           +TE+ LSILLKLCKSGE+ E++VRVEAAQLGAFQKILVLLQVGCGG MKDKVTE+LKLLN
Sbjct: 361 ATEYSLSILLKLCKSGEKGENEVRVEAAQLGAFQKILVLLQVGCGGDMKDKVTEMLKLLN 420

Query: 421 LYKDRVDCIDSSMHFKYLKKSF 443
           LYKDR+DCIDSSMHFKYLKKSF
Sbjct: 421 LYKDRLDCIDSSMHFKYLKKSF 442

BLAST of MS019154 vs. NCBI nr
Match: XP_022997974.1 (U-box domain-containing protein 21-like [Cucurbita maxima])

HSP 1 Score: 761.1 bits (1964), Expect = 5.1e-216
Identity = 382/443 (86.23%), Postives = 415/443 (93.68%), Query Frame = 0

Query: 1   MILSWRKNKLGLLSKLKKE-VPGVDSGAEIEIPSHFLCPISLDLMKDPVILCTGITYDRE 60
           M LSWRK+KLG L KLKKE +PGVDSG+EI IPSHFLCPISLDLMKDPVIL TGITYDRE
Sbjct: 1   MFLSWRKSKLGFLPKLKKEHLPGVDSGSEIVIPSHFLCPISLDLMKDPVILSTGITYDRE 60

Query: 61  SIEKWIDAGNFLCPVTKQGLTGFDLIPNHALRRMIQDWCVANRSYGIERIPTPRIPVTPY 120
           SIEKWI+ GNF CPVTKQ L  FDLIPNH LRRMIQDWCVAN SYGIERIPTPRIPVTPY
Sbjct: 61  SIEKWIEGGNFSCPVTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPY 120

Query: 121 EVSEICSRIAAATQRTDSKRCGELVGKTRNWGRESERNRRCIVSGGTGNVLAASFEHFAG 180
           EV+++CSRIA ATQR DSKRC ELVGK RNW RESERNRRC+V+GGTG VLAASFEHFAG
Sbjct: 121 EVNDVCSRIAVATQRGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHFAG 180

Query: 181 VSIEKHAGLLEEILLVLVWMFPIAMEGLSKLGSADSLKCLVSFLVGKDLSPKKSAVFVLK 240
           VSIEKH GLLEEILLVLV +FPI++EGLSKLGSADSLKCLVSFLVGK+LSPK++AVFVLK
Sbjct: 181 VSIEKHVGLLEEILLVLVCVFPISIEGLSKLGSADSLKCLVSFLVGKNLSPKQNAVFVLK 240

Query: 241 ELLLVDQRFVDSLAAIEGVPEALVSIVKEPLCSSATRSSLTAIFYMILPSEISEKMALKF 300
           ELLLVDQR+VDSLAAIEGV EALVSI++EPLC SAT+SSLTAIFYMILPSEISEKM +KF
Sbjct: 241 ELLLVDQRYVDSLAAIEGVSEALVSIIREPLCPSATKSSLTAIFYMILPSEISEKMTMKF 300

Query: 301 VELGLVSLLLEFLVDAEKSLCEKALGILDGICDFKQGREKLYQNALTIPLLVKKILRVSE 360
           +ELGLVS LL+FLVDAEKSLCEKALG+LDGICDFKQGREKLYQNALTIPLLVKKILRVSE
Sbjct: 301 MELGLVSQLLDFLVDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSE 360

Query: 361 LSTEFCLSILLKLCKSGERDEDDVRVEAAQLGAFQKILVLLQVGCGGAMKDKVTELLKLL 420
           +++E+CLSILLKLCKSGE+ ED+ RVEAAQLGAFQKI+VLLQVGCGG MKDKVTE+LKLL
Sbjct: 361 VASEYCLSILLKLCKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMKDKVTEMLKLL 420

Query: 421 NLYKDRVDCIDSSMHFKYLKKSF 443
           NLYKDR+DCIDSSMHFK+LKKSF
Sbjct: 421 NLYKDRLDCIDSSMHFKFLKKSF 443

BLAST of MS019154 vs. NCBI nr
Match: XP_023523202.1 (U-box domain-containing protein 21-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 759.2 bits (1959), Expect = 2.0e-215
Identity = 381/443 (86.00%), Postives = 415/443 (93.68%), Query Frame = 0

Query: 1   MILSWRKNKLGLLSKLKKE-VPGVDSGAEIEIPSHFLCPISLDLMKDPVILCTGITYDRE 60
           M LSWR++KLG L KLKKE +PGVDSG+EI IPSHFLCPISLDLMKDPVIL TGITYDRE
Sbjct: 1   MFLSWRRSKLGFLPKLKKEHLPGVDSGSEIVIPSHFLCPISLDLMKDPVILSTGITYDRE 60

Query: 61  SIEKWIDAGNFLCPVTKQGLTGFDLIPNHALRRMIQDWCVANRSYGIERIPTPRIPVTPY 120
           SIEKWI+ GNF CPVTKQ L  FDLIPNH LRRMIQDWCVAN SYGIERIPTPRIPVTPY
Sbjct: 61  SIEKWIEGGNFSCPVTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPY 120

Query: 121 EVSEICSRIAAATQRTDSKRCGELVGKTRNWGRESERNRRCIVSGGTGNVLAASFEHFAG 180
           EV+++CSRIA ATQR DSKRC ELVGK RNW RESERNRRC+V+GGTG VLAASFEHFAG
Sbjct: 121 EVNDVCSRIAVATQRGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHFAG 180

Query: 181 VSIEKHAGLLEEILLVLVWMFPIAMEGLSKLGSADSLKCLVSFLVGKDLSPKKSAVFVLK 240
           VSIEKH GLLEEILLVLV +FPI++EGLSKLGSADSLKCLVSFL+GK+LSPK++AVFVLK
Sbjct: 181 VSIEKHVGLLEEILLVLVCVFPISIEGLSKLGSADSLKCLVSFLMGKNLSPKQNAVFVLK 240

Query: 241 ELLLVDQRFVDSLAAIEGVPEALVSIVKEPLCSSATRSSLTAIFYMILPSEISEKMALKF 300
           ELLLVDQR+VDSLAAIEGV EALVSI++EPLC SAT+SSLTAIFYMILPSEISEKM LKF
Sbjct: 241 ELLLVDQRYVDSLAAIEGVSEALVSIIREPLCPSATKSSLTAIFYMILPSEISEKMTLKF 300

Query: 301 VELGLVSLLLEFLVDAEKSLCEKALGILDGICDFKQGREKLYQNALTIPLLVKKILRVSE 360
           +ELGLVS LL+FLVDAEKSLCEKALG+LDGICDFKQGREKLYQNALTIPLLVKKILRVSE
Sbjct: 301 MELGLVSELLDFLVDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSE 360

Query: 361 LSTEFCLSILLKLCKSGERDEDDVRVEAAQLGAFQKILVLLQVGCGGAMKDKVTELLKLL 420
           +++E+CLSILLKLCKSGE+ ED+ RVEAAQLGAFQKI+VLLQVGCGG MKDKVTE+LKLL
Sbjct: 361 VASEYCLSILLKLCKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMKDKVTEMLKLL 420

Query: 421 NLYKDRVDCIDSSMHFKYLKKSF 443
           NLYKDR+DCIDSSMHFK+LKKSF
Sbjct: 421 NLYKDRLDCIDSSMHFKFLKKSF 443

BLAST of MS019154 vs. ExPASy Swiss-Prot
Match: Q5PNY6 (U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 2.7e-111
Identity = 215/424 (50.71%), Postives = 296/424 (69.81%), Query Frame = 0

Query: 21  PGVDSGAEIEIPSHFLCPISLDLMKDPVILCTGITYDRESIEKWIDAGNFLCPVTKQGLT 80
           P  +  +EI IP  F CPIS+DLMKDPVI+ TGITYDR SIE WI++GN  CPVT   LT
Sbjct: 21  PESEPESEITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKTCPVTNTVLT 80

Query: 81  GFDLIPNHALRRMIQDWCVANRSYGIERIPTPRIPVTPYEVSEICSRIAAATQRTDSKRC 140
            FD IPNH +R+MIQ WCV   S  I+RIPTPR+P+ P EV EI  ++++AT+R D ++C
Sbjct: 81  TFDQIPNHTIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKC 140

Query: 141 GELVGKTRNWGRESERNRRCIVSGGTGNVLAASFEHFAGVSIEKHAGLLEEILLVLVWMF 200
           G ++ K +  G ESE+NR+C+     G VL   F+ F+G   EK   +L EIL +L WMF
Sbjct: 141 GVIIEKIKKLGDESEKNRKCVNENSVGWVLCDCFDKFSG--DEKLTFMLNEILSLLTWMF 200

Query: 201 PIAMEGLSKLGSADSLKCLVSFLVGKDLSPKKSAVFVLKELLLVDQRFVDSLAAIEGVPE 260
           PI +EG+SKL SA S +C+   L   D S +++A F++KE+L +D+  V S A   GV E
Sbjct: 201 PIGLEGISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGVAE 260

Query: 261 ALVSIVKEPLCSSATRSSLTAIFYMILPSEISEKMALKFVELGLVSLLLEFLVDAEKSLC 320
           ALV ++++ + SS+T+SSL AI+ M+L      ++A +F+E+GLVS+ +E +VDAE S+C
Sbjct: 261 ALVKLIRDSVSSSSTKSSLIAIYQMVLQ---KPEIASEFLEIGLVSITVEMIVDAENSVC 320

Query: 321 EKALGILDGICDFKQGREKLYQNALTIPLLVKKILRVSELSTEFCLSILLKLCKSGERDE 380
           EKAL +LD IC+ + GRE++ +NAL +PLLVKKI +VSEL+T   +S++LKL K+G    
Sbjct: 321 EKALAVLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTG---- 380

Query: 381 DDVRVE-AAQLGAFQKILVLLQVGCGGAMKDKVTELLKLLNL-YKDRVDCIDSSMHFKYL 440
           + V VE A +LGAFQK+L++LQVG G   K+K TELLK++N   K   DC+DS   FKY+
Sbjct: 381 NTVAVEDAVRLGAFQKVLLVLQVGYGEETKEKATELLKMMNTQMKLMSDCVDSLKEFKYI 435

Query: 441 KKSF 443
           KK F
Sbjct: 441 KKPF 435

BLAST of MS019154 vs. ExPASy Swiss-Prot
Match: Q9C8D1 (U-box domain-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=PUB20 PE=1 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 4.5e-98
Identity = 210/451 (46.56%), Postives = 289/451 (64.08%), Query Frame = 0

Query: 1   MILSWRKNKLGLLSKLKKEVPGVDSGAEIE--IPSHFLCPISLDLMKDPVILCTGITYDR 60
           M LS R  + G     K+ +P      E+E  IPS F CPIS +LMKDPVI+ +GITYDR
Sbjct: 1   MGLSLRVRRRGGSVSKKEIIPVTSCSEEVEITIPSQFQCPISYELMKDPVIIASGITYDR 60

Query: 61  ESIEKWIDAGNFLCPVTKQGLTGFDLIPNHALRRMIQDWCVANRSYGIERIPTPRIPVTP 120
           E+IEKW ++G   CPVT   LT  + IPNH +RRMIQ WC ++   GIERIPTPR+PVT 
Sbjct: 61  ENIEKWFESGYQTCPVTNTVLTSLEQIPNHTIRRMIQGWCGSSLGGGIERIPTPRVPVTS 120

Query: 121 YEVSEICSRIAAATQRTDSKRCGELVGKTRNWGRESERNRRCIVSGGTGNVLAASFEHF- 180
           ++VSEIC R++AAT+R D   C E+V K    G+ESERNR+C+   G G VL   F+ F 
Sbjct: 121 HQVSEICERLSAATRRGDYAACMEMVTKMTRLGKESERNRKCVKENGAGLVLCVCFDAFS 180

Query: 181 --AGVSIEKHAGLLEEILLVLVWMFPIAMEGLSKLGSADSLKCLVSFLVGKDLSPKKSAV 240
             A  S+     LLEE + VL WM PI +EG SKL +  S   LV  L   D    ++A 
Sbjct: 181 ENANASL-----LLEETVSVLTWMLPIGLEGQSKLTTTSSFNRLVELLRNGD----QNAA 240

Query: 241 FVLKELLLVDQRFVDSLAAIEGVPEALVSIVKEPLCSSATRSSLTAIFYMILPSEISEKM 300
           F++KELL ++   V +L  I GV EA +  +      S   +SL +I +MIL    +++ 
Sbjct: 241 FLIKELLELNVTHVHALTKINGVQEAFMKSINR---DSTCVNSLISIHHMIL---TNQET 300

Query: 301 ALKFVELGLVSLLLEFLVDAEKSLCEKALGILDGICDFKQGREKLYQNALTIPLLVKKIL 360
             +F+EL LV++ +E LVD+E S+CEKAL +L+ IC+ K+GREK+ +N L IP+LVKKIL
Sbjct: 301 VSRFLELDLVNITVEMLVDSENSVCEKALTVLNVICETKEGREKVRRNKLVIPILVKKIL 360

Query: 361 RVSELSTEFCLSILLKLCKSGERDEDDVRVEAAQLGAFQKILVLLQVGCGGAMKDKVTEL 420
           ++SE   +  +S++ K+CKSG+  E +   EA +LGAF+K++V+LQVGCG   K+KVTEL
Sbjct: 361 KISE--KKDLVSVMWKVCKSGDGSEVE---EALRLGAFKKLVVMLQVGCGEGTKEKVTEL 420

Query: 421 LKLLNLYKDRVDCID----SSMHFKYLKKSF 443
           LK++N        +D    SS+ FK++KK F
Sbjct: 421 LKMMNKVMKMNGFVDRSYSSSIEFKHVKKPF 431

BLAST of MS019154 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 2.6e-53
Identity = 144/398 (36.18%), Postives = 212/398 (53.27%), Query Frame = 0

Query: 29  IEIPSHFLCPISLDLMKDPVILCTGITYDRESIEKWIDAGNFLCPVTKQGLTGFDLIPNH 88
           I+IP HF CPISLDLM DPV + TG TYDR SI+ WI  GN  CPVT+  L+ F LIPNH
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCPVTRVALSDFTLIPNH 71

Query: 89  ALRRMIQDWCVANRSYGIERIPTPRIPVTPYEVSEICSRIAAATQRTDSKRC-GELVGKT 148
            LRR+IQ+WCVANRS G+ERIPTP+ P  P  V  + S+ +A T    S R     + + 
Sbjct: 72  TLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIRRL 131

Query: 149 RNWGRESERNRRCIVSGGTGNVLAASFEHFAGV-SIEKHAGLLEEILLVLVWMFPIAMEG 208
           R   R+SE+NR  I       +L      FA + +    + L+ E L +LV +     E 
Sbjct: 132 RGLARDSEKNRVLIAGHNAREILVRIL--FADIETTSLSSELVSESLALLVLLHMTETEC 191

Query: 209 LSKLGSADSLKCLVSFLVGKDLSPKKSAVFVLKELLLVDQRFVDSLAAIEG---VPEALV 268
            +       +  +   L    +  + +A   L E++L   + +D    I G   + E ++
Sbjct: 192 EAVASDPSRVGFMTRLLFDSSIEIRVNAA-ALIEMVLTGAKSMDLKLIISGSDSIFEGVL 251

Query: 269 SIVKEPLCS-SATRSSLTAIFYMILPSEISEKMALKFVELGLVSLLLEFL-VDAEKSLCE 328
            ++K P+ S  A +  + AIF + L  + +  +A   +  G   +L++ L  D ++   E
Sbjct: 252 DLLKNPISSRRALKIGIKAIFALCLVKQ-TRHLA---ISAGAPGILIDRLAADFDRCDTE 311

Query: 329 KALGILDGICDFKQGREKLYQNALTIPLLVKKILRVSELSTEFCLSILLKLCKSGERDED 388
           + L  ++ +C   +G     ++ALT+PL+VK ILRVS+ +TE+    LL LC + ER  D
Sbjct: 312 RGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALCTAEERCRD 371

Query: 389 DVRVEAAQLGAFQKILVLLQVGCGGAMKDKVTELLKLL 420
               EAA  G   ++L+L+Q  C    K K   LLKLL
Sbjct: 372 ----EAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLL 398

BLAST of MS019154 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 2.4e-51
Identity = 149/426 (34.98%), Postives = 227/426 (53.29%), Query Frame = 0

Query: 28  EIEIPSHFLCPISLDLMKDPVILCTGITYDRESIEKWIDAG-NFLCPVTKQGLTGFDLIP 87
           EIEIP  FLCPISL++MKDPVI+ TGITYDR+SIEKW+ AG    CPVTKQ +T  DL P
Sbjct: 9   EIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTP 68

Query: 88  NHALRRMIQDWCVANRSYGIERIPTPRIPVTPYEVSEICSRIAAATQRTDSKRCGELVGK 147
           NH LRR+IQ WC  N SYG+ERIPTPR P+   E+ ++     +A+   +  +C   + +
Sbjct: 69  NHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIR--DSASSHENQVKC---LKR 128

Query: 148 TRNWGRESERNRRCIVSGGTGNVLAASFEHFAGVSIEKHAGLLEEILLVLVWMFPIAMEG 207
            R    E+  N+RC+ + G    LA        VS +   G L +  L L++    +   
Sbjct: 129 LRQIVSENATNKRCLEAAGVPEFLANI------VSNDSENGSLTDEALNLLYHLETSETV 188

Query: 208 LSKL----GSADSLKCLVSFLVGKDLSPKKSAVFVLKELLLVDQRFVDSLAAIEGVPEA- 267
           L  L       + +K L   +       +  A  +LK +L V     D + ++   PE  
Sbjct: 189 LKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEV----ADPMQSMTLKPEVF 248

Query: 268 --LVSIVKEPLCSSATRSSLTAIFYMILPSEISEKMALKFVELGLVSLLLEFLVD----A 327
             +V I+ + +   AT++++  I   I P   +     K VE G++S+++E L+D    +
Sbjct: 249 TEVVQILDDRISQKATKAAM-HILVNICPWGRNRH---KAVEAGVISVIIELLMDESFTS 308

Query: 328 EKSLCEKALGILDGICDFKQGREKLYQNALTIPLLVKKILRVSELSTEFCLSILL---KL 387
           E+   E A+ +LD +C   +GR +   +   I ++ KKILRVS+ +++  + +LL   + 
Sbjct: 309 ERRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRF 368

Query: 388 CKSGERDEDDVRVEAAQLGAFQKILVLLQVGCGGAMKDKVTELLKL-LNLYKDRVDCIDS 438
           C +       +  E  QLG   K+ ++LQV CGG  K+K  ELLKL   ++KD   C+  
Sbjct: 369 CAT-----PALLHEMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVWKDS-PCLPK 409

BLAST of MS019154 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 7.1e-51
Identity = 144/412 (34.95%), Postives = 219/412 (53.16%), Query Frame = 0

Query: 16  LKKEVPGVDSGAEIEIPSHFLCPISLDLMKDPVILCTGITYDRESIEKWIDAG-NFLCPV 75
           + + +  +D G  I+IP HF CPISL+LM+DPV +CTG TYDR SIE W+  G N  CPV
Sbjct: 1   MPRNIEPLDLG--IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPV 60

Query: 76  TKQGLTGFDLIPNHALRRMIQDWCVANRSYGIERIPTPRIPVTPYEVSEICSRIAAATQR 135
           T+  L+ F LIPNH LRR+IQ+WCVANRS G+ERIPTP+ P  P  V  + S+ +A T  
Sbjct: 61  TRAPLSDFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGT 120

Query: 136 TDSKRC-GELVGKTRNWGRESERNRRCIVSGGTGNVLAASFEHFAGVSIEKHAGLLEEIL 195
             S R     + + R + R+S++NR  I +     +L          S    + L+ E L
Sbjct: 121 HVSVRSRAAALRRLRGFARDSDKNRVLIAAHNATEILIKIL-----FSETTSSELVSESL 180

Query: 196 LVLVWMFPIAMEGLSKLGSAD--SLKCLVSFLVGKDLSPKKSAVFVLKELLLVDQRFVD- 255
            +LV M PI         S+D   ++ L   L    +  + +A   L E++    +  D 
Sbjct: 181 ALLV-MLPITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAA-ALIEIVSTGTKSADL 240

Query: 256 --SLAAIEGVPEALVSIVKEPLCSSATRSSLTAIFYMILPSEISEKMALKFVELGLVSLL 315
             S++  E V E ++ +++ P+ S           + +   + +  +A   +  G   +L
Sbjct: 241 KGSISNSESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIA---ITAGAPEIL 300

Query: 316 LEFL-VDAEKSLCEKALGILDGICDFKQGREKLYQNALTIPLLVKKILRVSELSTEFCLS 375
           ++ L  D ++   E+AL  ++ +C   +G     ++ALT+PLLVK ILRVS+ +TE+   
Sbjct: 301 IDRLAADFDRCDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAG 360

Query: 376 ILLKLCKSGERDEDDVRVEAAQLGAFQKILVLLQVGCGGAMKDKVTELLKLL 420
            LL LC + ER     R EAA  G   ++L+++Q  C    K K  +LLKLL
Sbjct: 361 ALLALCTAEER----WREEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLL 396

BLAST of MS019154 vs. ExPASy TrEMBL
Match: A0A6J1CPB4 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111013319 PE=4 SV=1)

HSP 1 Score: 863.2 bits (2229), Expect = 4.7e-247
Identity = 440/442 (99.55%), Postives = 441/442 (99.77%), Query Frame = 0

Query: 1   MILSWRKNKLGLLSKLKKEVPGVDSGAEIEIPSHFLCPISLDLMKDPVILCTGITYDRES 60
           MILSWRKNKLGLLSKLKKEVPGVDSGAEIEIPSHFLCPISLDLMKDPVILCTGITYDRES
Sbjct: 1   MILSWRKNKLGLLSKLKKEVPGVDSGAEIEIPSHFLCPISLDLMKDPVILCTGITYDRES 60

Query: 61  IEKWIDAGNFLCPVTKQGLTGFDLIPNHALRRMIQDWCVANRSYGIERIPTPRIPVTPYE 120
           IEKWIDAGNFLCPVTKQGLTGFDLIPNHALRRMIQDWCVANRSYGIERIPTPRIPVTPYE
Sbjct: 61  IEKWIDAGNFLCPVTKQGLTGFDLIPNHALRRMIQDWCVANRSYGIERIPTPRIPVTPYE 120

Query: 121 VSEICSRIAAATQRTDSKRCGELVGKTRNWGRESERNRRCIVSGGTGNVLAASFEHFAGV 180
           VSEICSRIAAATQRTDSKRCGELVGK RNWGRESERNRRCIVSGGTGNVLAASFEHFAGV
Sbjct: 121 VSEICSRIAAATQRTDSKRCGELVGKIRNWGRESERNRRCIVSGGTGNVLAASFEHFAGV 180

Query: 181 SIEKHAGLLEEILLVLVWMFPIAMEGLSKLGSADSLKCLVSFLVGKDLSPKKSAVFVLKE 240
           SIEKHAGLLEEILLVLVWMFPIAMEGLSKLGSADSLKCLVSFLVGKDLSPKKSAVFVLKE
Sbjct: 181 SIEKHAGLLEEILLVLVWMFPIAMEGLSKLGSADSLKCLVSFLVGKDLSPKKSAVFVLKE 240

Query: 241 LLLVDQRFVDSLAAIEGVPEALVSIVKEPLCSSATRSSLTAIFYMILPSEISEKMALKFV 300
           LLLVDQRFVDSLAAIEGVPEALVSIVKEPLCSSATRSSLTAIFYMILPSEISEKMALKF+
Sbjct: 241 LLLVDQRFVDSLAAIEGVPEALVSIVKEPLCSSATRSSLTAIFYMILPSEISEKMALKFM 300

Query: 301 ELGLVSLLLEFLVDAEKSLCEKALGILDGICDFKQGREKLYQNALTIPLLVKKILRVSEL 360
           ELGLVSLLLEFLVDAEKSLCEKALGILDGICDFKQGREKLYQNALTIPLLVKKILRVSEL
Sbjct: 301 ELGLVSLLLEFLVDAEKSLCEKALGILDGICDFKQGREKLYQNALTIPLLVKKILRVSEL 360

Query: 361 STEFCLSILLKLCKSGERDEDDVRVEAAQLGAFQKILVLLQVGCGGAMKDKVTELLKLLN 420
           STEFCLSILLKLCKSGERDEDDVRVEAAQLGAFQKILVLLQVGCGGAMKDKVTELLKLLN
Sbjct: 361 STEFCLSILLKLCKSGERDEDDVRVEAAQLGAFQKILVLLQVGCGGAMKDKVTELLKLLN 420

Query: 421 LYKDRVDCIDSSMHFKYLKKSF 443
           LYKDRVDCIDSSMHFKYLKKSF
Sbjct: 421 LYKDRVDCIDSSMHFKYLKKSF 442

BLAST of MS019154 vs. ExPASy TrEMBL
Match: A0A6J1CNU1 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111013319 PE=4 SV=1)

HSP 1 Score: 803.5 bits (2074), Expect = 4.4e-229
Identity = 410/412 (99.51%), Postives = 411/412 (99.76%), Query Frame = 0

Query: 1   MILSWRKNKLGLLSKLKKEVPGVDSGAEIEIPSHFLCPISLDLMKDPVILCTGITYDRES 60
           MILSWRKNKLGLLSKLKKEVPGVDSGAEIEIPSHFLCPISLDLMKDPVILCTGITYDRES
Sbjct: 1   MILSWRKNKLGLLSKLKKEVPGVDSGAEIEIPSHFLCPISLDLMKDPVILCTGITYDRES 60

Query: 61  IEKWIDAGNFLCPVTKQGLTGFDLIPNHALRRMIQDWCVANRSYGIERIPTPRIPVTPYE 120
           IEKWIDAGNFLCPVTKQGLTGFDLIPNHALRRMIQDWCVANRSYGIERIPTPRIPVTPYE
Sbjct: 61  IEKWIDAGNFLCPVTKQGLTGFDLIPNHALRRMIQDWCVANRSYGIERIPTPRIPVTPYE 120

Query: 121 VSEICSRIAAATQRTDSKRCGELVGKTRNWGRESERNRRCIVSGGTGNVLAASFEHFAGV 180
           VSEICSRIAAATQRTDSKRCGELVGK RNWGRESERNRRCIVSGGTGNVLAASFEHFAGV
Sbjct: 121 VSEICSRIAAATQRTDSKRCGELVGKIRNWGRESERNRRCIVSGGTGNVLAASFEHFAGV 180

Query: 181 SIEKHAGLLEEILLVLVWMFPIAMEGLSKLGSADSLKCLVSFLVGKDLSPKKSAVFVLKE 240
           SIEKHAGLLEEILLVLVWMFPIAMEGLSKLGSADSLKCLVSFLVGKDLSPKKSAVFVLKE
Sbjct: 181 SIEKHAGLLEEILLVLVWMFPIAMEGLSKLGSADSLKCLVSFLVGKDLSPKKSAVFVLKE 240

Query: 241 LLLVDQRFVDSLAAIEGVPEALVSIVKEPLCSSATRSSLTAIFYMILPSEISEKMALKFV 300
           LLLVDQRFVDSLAAIEGVPEALVSIVKEPLCSSATRSSLTAIFYMILPSEISEKMALKF+
Sbjct: 241 LLLVDQRFVDSLAAIEGVPEALVSIVKEPLCSSATRSSLTAIFYMILPSEISEKMALKFM 300

Query: 301 ELGLVSLLLEFLVDAEKSLCEKALGILDGICDFKQGREKLYQNALTIPLLVKKILRVSEL 360
           ELGLVSLLLEFLVDAEKSLCEKALGILDGICDFKQGREKLYQNALTIPLLVKKILRVSEL
Sbjct: 301 ELGLVSLLLEFLVDAEKSLCEKALGILDGICDFKQGREKLYQNALTIPLLVKKILRVSEL 360

Query: 361 STEFCLSILLKLCKSGERDEDDVRVEAAQLGAFQKILVLLQVGCGGAMKDKV 413
           STEFCLSILLKLCKSGERDEDDVRVEAAQLGAFQKILVLLQVGCGGAMKDKV
Sbjct: 361 STEFCLSILLKLCKSGERDEDDVRVEAAQLGAFQKILVLLQVGCGGAMKDKV 412

BLAST of MS019154 vs. ExPASy TrEMBL
Match: A0A0A0LAV4 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G308190 PE=4 SV=1)

HSP 1 Score: 763.5 bits (1970), Expect = 5.0e-217
Identity = 381/442 (86.20%), Postives = 416/442 (94.12%), Query Frame = 0

Query: 1   MILSWRKNKLGLLSKLKKEVPGVDSGAEIEIPSHFLCPISLDLMKDPVILCTGITYDRES 60
           MI SWRKNKLG L+KLKKE+PGVDSG+EI IPSH++CPISLDLMKDPVIL TGITYDRES
Sbjct: 1   MIFSWRKNKLGFLTKLKKELPGVDSGSEIAIPSHYMCPISLDLMKDPVILSTGITYDRES 60

Query: 61  IEKWIDAGNFLCPVTKQGLTGFDLIPNHALRRMIQDWCVANRSYGIERIPTPRIPVTPYE 120
           IEKWID GNF CPVTKQ LT FDLIPNHALRR+IQDWCVANRSYGIERIPTPRIPV+PYE
Sbjct: 61  IEKWIDGGNFSCPVTKQDLTVFDLIPNHALRRLIQDWCVANRSYGIERIPTPRIPVSPYE 120

Query: 121 VSEICSRIAAATQRTDSKRCGELVGKTRNWGRESERNRRCIVSGGTGNVLAASFEHFAGV 180
           V EICSRI+ ATQR+DSKRC EL+GK RNW +ESERNRRCIV+GGTG+VLAASFEHFAGV
Sbjct: 121 VKEICSRISIATQRSDSKRCSELMGKIRNWAKESERNRRCIVNGGTGDVLAASFEHFAGV 180

Query: 181 SIEKHAGLLEEILLVLVWMFPIAMEGLSKLGSADSLKCLVSFLVGKDLSPKKSAVFVLKE 240
           SIEKH GLLEEILLVL  ++P+A+EGLSKLGSADSLKCLVSFLVGKDLSPK+SA+FVLKE
Sbjct: 181 SIEKHVGLLEEILLVLTCVYPVAIEGLSKLGSADSLKCLVSFLVGKDLSPKQSAIFVLKE 240

Query: 241 LLLVDQRFVDSLAAIEGVPEALVSIVKEPLCSSATRSSLTAIFYMILPSEISEKMALKFV 300
           LL  D+R+V+SLAAIEGV EALVSI+++PLC SAT+SSLTAIFYMILPS+I EKMALKFV
Sbjct: 241 LLAADRRYVNSLAAIEGVSEALVSIIRDPLCPSATKSSLTAIFYMILPSDIGEKMALKFV 300

Query: 301 ELGLVSLLLEFLVDAEKSLCEKALGILDGICDFKQGREKLYQNALTIPLLVKKILRVSEL 360
           ELGLVS LLEFLVDAEKSLCEKALGILDGICD+KQGREKLY NALTIPLLVKKILRVSEL
Sbjct: 301 ELGLVSQLLEFLVDAEKSLCEKALGILDGICDYKQGREKLYNNALTIPLLVKKILRVSEL 360

Query: 361 STEFCLSILLKLCKSGERDEDDVRVEAAQLGAFQKILVLLQVGCGGAMKDKVTELLKLLN 420
           +TE+ LSILLKLCKSGE+ E++VRVEAAQLGAFQKILVLLQVGCGG MKDKVTE+LKLLN
Sbjct: 361 ATEYSLSILLKLCKSGEKGENEVRVEAAQLGAFQKILVLLQVGCGGDMKDKVTEMLKLLN 420

Query: 421 LYKDRVDCIDSSMHFKYLKKSF 443
           LYKDR+DCIDSSMHFKYLKKSF
Sbjct: 421 LYKDRLDCIDSSMHFKYLKKSF 442

BLAST of MS019154 vs. ExPASy TrEMBL
Match: A0A6J1KD23 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492767 PE=4 SV=1)

HSP 1 Score: 761.1 bits (1964), Expect = 2.5e-216
Identity = 382/443 (86.23%), Postives = 415/443 (93.68%), Query Frame = 0

Query: 1   MILSWRKNKLGLLSKLKKE-VPGVDSGAEIEIPSHFLCPISLDLMKDPVILCTGITYDRE 60
           M LSWRK+KLG L KLKKE +PGVDSG+EI IPSHFLCPISLDLMKDPVIL TGITYDRE
Sbjct: 1   MFLSWRKSKLGFLPKLKKEHLPGVDSGSEIVIPSHFLCPISLDLMKDPVILSTGITYDRE 60

Query: 61  SIEKWIDAGNFLCPVTKQGLTGFDLIPNHALRRMIQDWCVANRSYGIERIPTPRIPVTPY 120
           SIEKWI+ GNF CPVTKQ L  FDLIPNH LRRMIQDWCVAN SYGIERIPTPRIPVTPY
Sbjct: 61  SIEKWIEGGNFSCPVTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPY 120

Query: 121 EVSEICSRIAAATQRTDSKRCGELVGKTRNWGRESERNRRCIVSGGTGNVLAASFEHFAG 180
           EV+++CSRIA ATQR DSKRC ELVGK RNW RESERNRRC+V+GGTG VLAASFEHFAG
Sbjct: 121 EVNDVCSRIAVATQRGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHFAG 180

Query: 181 VSIEKHAGLLEEILLVLVWMFPIAMEGLSKLGSADSLKCLVSFLVGKDLSPKKSAVFVLK 240
           VSIEKH GLLEEILLVLV +FPI++EGLSKLGSADSLKCLVSFLVGK+LSPK++AVFVLK
Sbjct: 181 VSIEKHVGLLEEILLVLVCVFPISIEGLSKLGSADSLKCLVSFLVGKNLSPKQNAVFVLK 240

Query: 241 ELLLVDQRFVDSLAAIEGVPEALVSIVKEPLCSSATRSSLTAIFYMILPSEISEKMALKF 300
           ELLLVDQR+VDSLAAIEGV EALVSI++EPLC SAT+SSLTAIFYMILPSEISEKM +KF
Sbjct: 241 ELLLVDQRYVDSLAAIEGVSEALVSIIREPLCPSATKSSLTAIFYMILPSEISEKMTMKF 300

Query: 301 VELGLVSLLLEFLVDAEKSLCEKALGILDGICDFKQGREKLYQNALTIPLLVKKILRVSE 360
           +ELGLVS LL+FLVDAEKSLCEKALG+LDGICDFKQGREKLYQNALTIPLLVKKILRVSE
Sbjct: 301 MELGLVSQLLDFLVDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSE 360

Query: 361 LSTEFCLSILLKLCKSGERDEDDVRVEAAQLGAFQKILVLLQVGCGGAMKDKVTELLKLL 420
           +++E+CLSILLKLCKSGE+ ED+ RVEAAQLGAFQKI+VLLQVGCGG MKDKVTE+LKLL
Sbjct: 361 VASEYCLSILLKLCKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMKDKVTEMLKLL 420

Query: 421 NLYKDRVDCIDSSMHFKYLKKSF 443
           NLYKDR+DCIDSSMHFK+LKKSF
Sbjct: 421 NLYKDRLDCIDSSMHFKFLKKSF 443

BLAST of MS019154 vs. ExPASy TrEMBL
Match: A0A6J1GCB6 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111452870 PE=4 SV=1)

HSP 1 Score: 757.3 bits (1954), Expect = 3.6e-215
Identity = 379/443 (85.55%), Postives = 415/443 (93.68%), Query Frame = 0

Query: 1   MILSWRKNKLGLLSKLKKE-VPGVDSGAEIEIPSHFLCPISLDLMKDPVILCTGITYDRE 60
           M LSWR++KLG L KLKKE +PGVDSG+EI IPSHFLCPISLDLMKDPVIL TGITYDRE
Sbjct: 1   MFLSWRRSKLGFLPKLKKEHLPGVDSGSEIVIPSHFLCPISLDLMKDPVILSTGITYDRE 60

Query: 61  SIEKWIDAGNFLCPVTKQGLTGFDLIPNHALRRMIQDWCVANRSYGIERIPTPRIPVTPY 120
           SIEKWI+ GNF CPVTKQ L  FDLIPNH LRRMIQDWCVAN SYGIERIPTPRIPVTPY
Sbjct: 61  SIEKWIEGGNFSCPVTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPY 120

Query: 121 EVSEICSRIAAATQRTDSKRCGELVGKTRNWGRESERNRRCIVSGGTGNVLAASFEHFAG 180
           EV+++CSRIA ATQR DSKRC ELVGK RNW RESERNRRC+V+GGTG VLAASFEHFAG
Sbjct: 121 EVNDVCSRIAVATQRGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHFAG 180

Query: 181 VSIEKHAGLLEEILLVLVWMFPIAMEGLSKLGSADSLKCLVSFLVGKDLSPKKSAVFVLK 240
           VSIEKH GLLEEILLVLV +FPI++EGLSKLGSADSLKC+VSFL+GK+LSPK++AVFVLK
Sbjct: 181 VSIEKHVGLLEEILLVLVCVFPISIEGLSKLGSADSLKCMVSFLMGKNLSPKQNAVFVLK 240

Query: 241 ELLLVDQRFVDSLAAIEGVPEALVSIVKEPLCSSATRSSLTAIFYMILPSEISEKMALKF 300
           ELLLVDQR+VDSLAAIEGV EALVSI++EPLC SAT+SSLTAIFY+ILPSEISEKM LKF
Sbjct: 241 ELLLVDQRYVDSLAAIEGVSEALVSIIREPLCPSATKSSLTAIFYIILPSEISEKMTLKF 300

Query: 301 VELGLVSLLLEFLVDAEKSLCEKALGILDGICDFKQGREKLYQNALTIPLLVKKILRVSE 360
           +ELGLVS LL+FLVDAEKSLCEKALG+LDGICDFKQGREKLYQNALTIPLLVKKILRVSE
Sbjct: 301 MELGLVSQLLDFLVDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSE 360

Query: 361 LSTEFCLSILLKLCKSGERDEDDVRVEAAQLGAFQKILVLLQVGCGGAMKDKVTELLKLL 420
           +++E+CLSILLKLCKSGE+ ED+ RVEAAQLGAFQKI+VLLQVGCGG MKDKVTE+LKLL
Sbjct: 361 VASEYCLSILLKLCKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMKDKVTEMLKLL 420

Query: 421 NLYKDRVDCIDSSMHFKYLKKSF 443
           NLYKDR+DCIDSSMHFK+LKKSF
Sbjct: 421 NLYKDRLDCIDSSMHFKFLKKSF 443

BLAST of MS019154 vs. TAIR 10
Match: AT5G37490.1 (ARM repeat superfamily protein )

HSP 1 Score: 403.7 bits (1036), Expect = 1.9e-112
Identity = 215/424 (50.71%), Postives = 296/424 (69.81%), Query Frame = 0

Query: 21  PGVDSGAEIEIPSHFLCPISLDLMKDPVILCTGITYDRESIEKWIDAGNFLCPVTKQGLT 80
           P  +  +EI IP  F CPIS+DLMKDPVI+ TGITYDR SIE WI++GN  CPVT   LT
Sbjct: 21  PESEPESEITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKTCPVTNTVLT 80

Query: 81  GFDLIPNHALRRMIQDWCVANRSYGIERIPTPRIPVTPYEVSEICSRIAAATQRTDSKRC 140
            FD IPNH +R+MIQ WCV   S  I+RIPTPR+P+ P EV EI  ++++AT+R D ++C
Sbjct: 81  TFDQIPNHTIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKC 140

Query: 141 GELVGKTRNWGRESERNRRCIVSGGTGNVLAASFEHFAGVSIEKHAGLLEEILLVLVWMF 200
           G ++ K +  G ESE+NR+C+     G VL   F+ F+G   EK   +L EIL +L WMF
Sbjct: 141 GVIIEKIKKLGDESEKNRKCVNENSVGWVLCDCFDKFSG--DEKLTFMLNEILSLLTWMF 200

Query: 201 PIAMEGLSKLGSADSLKCLVSFLVGKDLSPKKSAVFVLKELLLVDQRFVDSLAAIEGVPE 260
           PI +EG+SKL SA S +C+   L   D S +++A F++KE+L +D+  V S A   GV E
Sbjct: 201 PIGLEGISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGVAE 260

Query: 261 ALVSIVKEPLCSSATRSSLTAIFYMILPSEISEKMALKFVELGLVSLLLEFLVDAEKSLC 320
           ALV ++++ + SS+T+SSL AI+ M+L      ++A +F+E+GLVS+ +E +VDAE S+C
Sbjct: 261 ALVKLIRDSVSSSSTKSSLIAIYQMVLQ---KPEIASEFLEIGLVSITVEMIVDAENSVC 320

Query: 321 EKALGILDGICDFKQGREKLYQNALTIPLLVKKILRVSELSTEFCLSILLKLCKSGERDE 380
           EKAL +LD IC+ + GRE++ +NAL +PLLVKKI +VSEL+T   +S++LKL K+G    
Sbjct: 321 EKALAVLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTG---- 380

Query: 381 DDVRVE-AAQLGAFQKILVLLQVGCGGAMKDKVTELLKLLNL-YKDRVDCIDSSMHFKYL 440
           + V VE A +LGAFQK+L++LQVG G   K+K TELLK++N   K   DC+DS   FKY+
Sbjct: 381 NTVAVEDAVRLGAFQKVLLVLQVGYGEETKEKATELLKMMNTQMKLMSDCVDSLKEFKYI 435

Query: 441 KKSF 443
           KK F
Sbjct: 441 KKPF 435

BLAST of MS019154 vs. TAIR 10
Match: AT1G66160.1 (CYS, MET, PRO, and GLY protein 1 )

HSP 1 Score: 359.8 bits (922), Expect = 3.2e-99
Identity = 210/451 (46.56%), Postives = 289/451 (64.08%), Query Frame = 0

Query: 1   MILSWRKNKLGLLSKLKKEVPGVDSGAEIE--IPSHFLCPISLDLMKDPVILCTGITYDR 60
           M LS R  + G     K+ +P      E+E  IPS F CPIS +LMKDPVI+ +GITYDR
Sbjct: 1   MGLSLRVRRRGGSVSKKEIIPVTSCSEEVEITIPSQFQCPISYELMKDPVIIASGITYDR 60

Query: 61  ESIEKWIDAGNFLCPVTKQGLTGFDLIPNHALRRMIQDWCVANRSYGIERIPTPRIPVTP 120
           E+IEKW ++G   CPVT   LT  + IPNH +RRMIQ WC ++   GIERIPTPR+PVT 
Sbjct: 61  ENIEKWFESGYQTCPVTNTVLTSLEQIPNHTIRRMIQGWCGSSLGGGIERIPTPRVPVTS 120

Query: 121 YEVSEICSRIAAATQRTDSKRCGELVGKTRNWGRESERNRRCIVSGGTGNVLAASFEHF- 180
           ++VSEIC R++AAT+R D   C E+V K    G+ESERNR+C+   G G VL   F+ F 
Sbjct: 121 HQVSEICERLSAATRRGDYAACMEMVTKMTRLGKESERNRKCVKENGAGLVLCVCFDAFS 180

Query: 181 --AGVSIEKHAGLLEEILLVLVWMFPIAMEGLSKLGSADSLKCLVSFLVGKDLSPKKSAV 240
             A  S+     LLEE + VL WM PI +EG SKL +  S   LV  L   D    ++A 
Sbjct: 181 ENANASL-----LLEETVSVLTWMLPIGLEGQSKLTTTSSFNRLVELLRNGD----QNAA 240

Query: 241 FVLKELLLVDQRFVDSLAAIEGVPEALVSIVKEPLCSSATRSSLTAIFYMILPSEISEKM 300
           F++KELL ++   V +L  I GV EA +  +      S   +SL +I +MIL    +++ 
Sbjct: 241 FLIKELLELNVTHVHALTKINGVQEAFMKSINR---DSTCVNSLISIHHMIL---TNQET 300

Query: 301 ALKFVELGLVSLLLEFLVDAEKSLCEKALGILDGICDFKQGREKLYQNALTIPLLVKKIL 360
             +F+EL LV++ +E LVD+E S+CEKAL +L+ IC+ K+GREK+ +N L IP+LVKKIL
Sbjct: 301 VSRFLELDLVNITVEMLVDSENSVCEKALTVLNVICETKEGREKVRRNKLVIPILVKKIL 360

Query: 361 RVSELSTEFCLSILLKLCKSGERDEDDVRVEAAQLGAFQKILVLLQVGCGGAMKDKVTEL 420
           ++SE   +  +S++ K+CKSG+  E +   EA +LGAF+K++V+LQVGCG   K+KVTEL
Sbjct: 361 KISE--KKDLVSVMWKVCKSGDGSEVE---EALRLGAFKKLVVMLQVGCGEGTKEKVTEL 420

Query: 421 LKLLNLYKDRVDCID----SSMHFKYLKKSF 443
           LK++N        +D    SS+ FK++KK F
Sbjct: 421 LKMMNKVMKMNGFVDRSYSSSIEFKHVKKPF 431

BLAST of MS019154 vs. TAIR 10
Match: AT1G66160.2 (CYS, MET, PRO, and GLY protein 1 )

HSP 1 Score: 323.6 bits (828), Expect = 2.6e-88
Identity = 198/451 (43.90%), Postives = 274/451 (60.75%), Query Frame = 0

Query: 1   MILSWRKNKLGLLSKLKKEVPGVDSGAEIE--IPSHFLCPISLDLMKDPVILCTGITYDR 60
           M LS R  + G     K+ +P      E+E  IPS F CPIS +LMKDP           
Sbjct: 1   MGLSLRVRRRGGSVSKKEIIPVTSCSEEVEITIPSQFQCPISYELMKDP----------- 60

Query: 61  ESIEKWIDAGNFLCPVTKQGLTGFDLIPNHALRRMIQDWCVANRSYGIERIPTPRIPVTP 120
                W ++G   CPVT   LT  + IPNH +RRMIQ WC ++   GIERIPTPR+PVT 
Sbjct: 61  -----WFESGYQTCPVTNTVLTSLEQIPNHTIRRMIQGWCGSSLGGGIERIPTPRVPVTS 120

Query: 121 YEVSEICSRIAAATQRTDSKRCGELVGKTRNWGRESERNRRCIVSGGTGNVLAASFEHF- 180
           ++VSEIC R++AAT+R D   C E+V K    G+ESERNR+C+   G G VL   F+ F 
Sbjct: 121 HQVSEICERLSAATRRGDYAACMEMVTKMTRLGKESERNRKCVKENGAGLVLCVCFDAFS 180

Query: 181 --AGVSIEKHAGLLEEILLVLVWMFPIAMEGLSKLGSADSLKCLVSFLVGKDLSPKKSAV 240
             A  S+     LLEE + VL WM PI +EG SKL +  S   LV  L   D    ++A 
Sbjct: 181 ENANASL-----LLEETVSVLTWMLPIGLEGQSKLTTTSSFNRLVELLRNGD----QNAA 240

Query: 241 FVLKELLLVDQRFVDSLAAIEGVPEALVSIVKEPLCSSATRSSLTAIFYMILPSEISEKM 300
           F++KELL ++   V +L  I GV EA +  +      S   +SL +I +MIL    +++ 
Sbjct: 241 FLIKELLELNVTHVHALTKINGVQEAFMKSINR---DSTCVNSLISIHHMIL---TNQET 300

Query: 301 ALKFVELGLVSLLLEFLVDAEKSLCEKALGILDGICDFKQGREKLYQNALTIPLLVKKIL 360
             +F+EL LV++ +E LVD+E S+CEKAL +L+ IC+ K+GREK+ +N L IP+LVKKIL
Sbjct: 301 VSRFLELDLVNITVEMLVDSENSVCEKALTVLNVICETKEGREKVRRNKLVIPILVKKIL 360

Query: 361 RVSELSTEFCLSILLKLCKSGERDEDDVRVEAAQLGAFQKILVLLQVGCGGAMKDKVTEL 420
           ++SE   +  +S++ K+CKSG+  E +   EA +LGAF+K++V+LQVGCG   K+KVTEL
Sbjct: 361 KISE--KKDLVSVMWKVCKSGDGSEVE---EALRLGAFKKLVVMLQVGCGEGTKEKVTEL 415

Query: 421 LKLLNLYKDRVDCID----SSMHFKYLKKSF 443
           LK++N        +D    SS+ FK++KK F
Sbjct: 421 LKMMNKVMKMNGFVDRSYSSSIEFKHVKKPF 415

BLAST of MS019154 vs. TAIR 10
Match: AT3G02840.1 (ARM repeat superfamily protein )

HSP 1 Score: 268.9 bits (686), Expect = 7.5e-72
Identity = 157/344 (45.64%), Postives = 231/344 (67.15%), Query Frame = 0

Query: 106 IERIPTPRIPVTPYEVSEICSRIAAATQRTDSKRCGELVGKTRNWGRESERNRRCIVSGG 165
           IERI +PR+ +TP +  EI  R+  A  R +   C E+V K +N GR  + N++C+V  G
Sbjct: 40  IERIQSPRVLLTPRDAVEISRRLQNAAAREEYAECLEIVSKIKNLGRGGDTNKKCLVQNG 99

Query: 166 TGNVLAASFEHFAGVSIEKHAGLLEEILLVLVWMFPI-AMEGLSKLGSADSLKCLVSFLV 225
           +   L++ FE FA    + H  LLEEIL VL    P+   EG +K+GS  SL CLV FL 
Sbjct: 100 SVLALSSCFERFAAAR-DGHMRLLEEILFVLSSWLPLNRSEGFNKMGSTASLNCLVRFLN 159

Query: 226 GKDLSPKKSAVFVLKELLLVDQRFVDSLAAIEGVPEALVSIVKEPLCSSATRSSLTAIFY 285
           GKD   +++A F ++E++ VD+R+V +L  +EG  E LV I+++ + +S+T++SL  I+ 
Sbjct: 160 GKDAKTRQNAAFCIREVIAVDKRYVYALTDVEGACEGLVKIIRDSVSTSSTKASLMVIYR 219

Query: 286 MILPSEISEKMALKFVELGLVSLLLEFLV-DAEKSLCEKALGILDGICDFKQGREKLYQN 345
            I     ++K+  KFV+LGLV L+ E +V +AEKS+CE++L +L+  CD +QG+E + +N
Sbjct: 220 AI---SSNDKITEKFVKLGLVELITEMMVNNAEKSVCERSLVVLNATCDNEQGKEDVLRN 279

Query: 346 ALTIPLLVKKILRVSELSTEFCLSILLKLCKSGERDEDDVR--VEAAQLGAFQKILVLLQ 405
           AL +PLLVKKILRVS+L+T+  +SIL KL K+ +  E D R  VEA Q+GAF+K+LVLLQ
Sbjct: 280 ALIVPLLVKKILRVSDLATQCSVSILWKLWKNKKDGECDDRLLVEALQVGAFEKLLVLLQ 339

Query: 406 VGCGGAMKDKVTELLKLLNLYKDRVD---CIDSSMHFKYLKKSF 443
           VGC    K+K +ELL+ LN  ++ ++   C+DSSMH K +KKSF
Sbjct: 340 VGCEDKTKEKASELLRNLNRCRNEIEKTNCVDSSMHLKNVKKSF 379

BLAST of MS019154 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 211.1 bits (536), Expect = 1.8e-54
Identity = 144/398 (36.18%), Postives = 212/398 (53.27%), Query Frame = 0

Query: 29  IEIPSHFLCPISLDLMKDPVILCTGITYDRESIEKWIDAGNFLCPVTKQGLTGFDLIPNH 88
           I+IP HF CPISLDLM DPV + TG TYDR SI+ WI  GN  CPVT+  L+ F LIPNH
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCPVTRVALSDFTLIPNH 71

Query: 89  ALRRMIQDWCVANRSYGIERIPTPRIPVTPYEVSEICSRIAAATQRTDSKRC-GELVGKT 148
            LRR+IQ+WCVANRS G+ERIPTP+ P  P  V  + S+ +A T    S R     + + 
Sbjct: 72  TLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIRRL 131

Query: 149 RNWGRESERNRRCIVSGGTGNVLAASFEHFAGV-SIEKHAGLLEEILLVLVWMFPIAMEG 208
           R   R+SE+NR  I       +L      FA + +    + L+ E L +LV +     E 
Sbjct: 132 RGLARDSEKNRVLIAGHNAREILVRIL--FADIETTSLSSELVSESLALLVLLHMTETEC 191

Query: 209 LSKLGSADSLKCLVSFLVGKDLSPKKSAVFVLKELLLVDQRFVDSLAAIEG---VPEALV 268
            +       +  +   L    +  + +A   L E++L   + +D    I G   + E ++
Sbjct: 192 EAVASDPSRVGFMTRLLFDSSIEIRVNAA-ALIEMVLTGAKSMDLKLIISGSDSIFEGVL 251

Query: 269 SIVKEPLCS-SATRSSLTAIFYMILPSEISEKMALKFVELGLVSLLLEFL-VDAEKSLCE 328
            ++K P+ S  A +  + AIF + L  + +  +A   +  G   +L++ L  D ++   E
Sbjct: 252 DLLKNPISSRRALKIGIKAIFALCLVKQ-TRHLA---ISAGAPGILIDRLAADFDRCDTE 311

Query: 329 KALGILDGICDFKQGREKLYQNALTIPLLVKKILRVSELSTEFCLSILLKLCKSGERDED 388
           + L  ++ +C   +G     ++ALT+PL+VK ILRVS+ +TE+    LL LC + ER  D
Sbjct: 312 RGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALCTAEERCRD 371

Query: 389 DVRVEAAQLGAFQKILVLLQVGCGGAMKDKVTELLKLL 420
               EAA  G   ++L+L+Q  C    K K   LLKLL
Sbjct: 372 ----EAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLL 398