MS019144 (gene) Bitter gourd (TR) v1

Overview


Name	MS019144
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	EF hand calcium-binding protein family
Location	scaffold20: 1889630 .. 1890034 (+)
RNA-Seq Expression	MS019144
Synteny	MS019144

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGGTCACCAAGGTGTTCAACAAGTTCGACAAGAACGGCGACGGCAAGATTTCCGTCAACGAGCTCGGCGACGCGCTCGGCGAACTCGGCGGCAAGGTCTCCTCCGACGAGGTCCGCCGCATTATGGCGGAGATCGATAAGGACGGCGACGGATTCATCGATCTCGACGAGTTCGCCGAGTTTCATCAAGACGTGTCGGCGACCGGCGGAGGAAACAAAGATCTGCAGGACGCTTTCGATCTGTACGACATCGACAAGAACGGCCTAATCTCGGCGAAGGAGTTGCATTCCGTGCTGAAGCGGCTCGGCGAGAAATGTAGCCTGAAGGATTGCTGCCGGATGATCAGTTCCGTCGATGTCGACGGCGACGGCCATGTAAATTTCGAGGAGTTCAAGAAGATGATG

mRNA sequence

GAGGTCACCAAGGTGTTCAACAAGTTCGACAAGAACGGCGACGGCAAGATTTCCGTCAACGAGCTCGGCGACGCGCTCGGCGAACTCGGCGGCAAGGTCTCCTCCGACGAGGTCCGCCGCATTATGGCGGAGATCGATAAGGACGGCGACGGATTCATCGATCTCGACGAGTTCGCCGAGTTTCATCAAGACGTGTCGGCGACCGGCGGAGGAAACAAAGATCTGCAGGACGCTTTCGATCTGTACGACATCGACAAGAACGGCCTAATCTCGGCGAAGGAGTTGCATTCCGTGCTGAAGCGGCTCGGCGAGAAATGTAGCCTGAAGGATTGCTGCCGGATGATCAGTTCCGTCGATGTCGACGGCGACGGCCATGTAAATTTCGAGGAGTTCAAGAAGATGATG

Coding sequence (CDS)

GAGGTCACCAAGGTGTTCAACAAGTTCGACAAGAACGGCGACGGCAAGATTTCCGTCAACGAGCTCGGCGACGCGCTCGGCGAACTCGGCGGCAAGGTCTCCTCCGACGAGGTCCGCCGCATTATGGCGGAGATCGATAAGGACGGCGACGGATTCATCGATCTCGACGAGTTCGCCGAGTTTCATCAAGACGTGTCGGCGACCGGCGGAGGAAACAAAGATCTGCAGGACGCTTTCGATCTGTACGACATCGACAAGAACGGCCTAATCTCGGCGAAGGAGTTGCATTCCGTGCTGAAGCGGCTCGGCGAGAAATGTAGCCTGAAGGATTGCTGCCGGATGATCAGTTCCGTCGATGTCGACGGCGACGGCCATGTAAATTTCGAGGAGTTCAAGAAGATGATG

Protein sequence

EVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEVRRIMAEIDKDGDGFIDLDEFAEFHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMM

Homology

[Close]

BLAST of MS019144 vs. NCBI nr
Match: XP_022143269.1 (probable calcium-binding protein CML23 [Momordica charantia])

HSP 1 Score: 273.5 bits (698), Expect = 9.9e-70
Identity = 135/135 (100.00%), Postives = 135/135 (100.00%), Query Frame = 0


Query: 1   EVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEVRRIMAEIDKDGDGFIDLDEFAE 60
           EVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEVRRIMAEIDKDGDGFIDLDEFAE
Sbjct: 25  EVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEVRRIMAEIDKDGDGFIDLDEFAE 84

Query: 61  FHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV 120
           FHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV
Sbjct: 85  FHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV 144

Query: 121 DGDGHVNFEEFKKMM 136
           DGDGHVNFEEFKKMM
Sbjct: 145 DGDGHVNFEEFKKMM 159

[Close]

BLAST of MS019144 vs. NCBI nr
Match: XP_038879983.1 (probable calcium-binding protein CML23 [Benincasa hispida])

HSP 1 Score: 251.5 bits (641), Expect = 4.0e-63
Identity = 122/135 (90.37%), Postives = 129/135 (95.56%), Query Frame = 0


Query: 1   EVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEVRRIMAEIDKDGDGFIDLDEFAE 60
           +V KVFNKFDKNGDGKISVNELG AL ELGGK+S+DE+RRIM+EIDKDGDGFIDLDEFAE
Sbjct: 22  DVIKVFNKFDKNGDGKISVNELGAALSELGGKISTDEIRRIMSEIDKDGDGFIDLDEFAE 81

Query: 61  FHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV 120
           FHQD S++ G NKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV
Sbjct: 82  FHQDASSSSGCNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV 141

Query: 121 DGDGHVNFEEFKKMM 136
           DGDGHVNFEEFKKMM
Sbjct: 142 DGDGHVNFEEFKKMM 156

[Close]

BLAST of MS019144 vs. NCBI nr
Match: XP_022948508.1 (probable calcium-binding protein CML23 [Cucurbita moschata] >XP_023524703.1 probable calcium-binding protein CML23 [Cucurbita pepo subsp. pepo] >KAG6607012.1 putative calcium-binding protein CML23, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036709.1 putative calcium-binding protein CML23, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 245.4 bits (625), Expect = 2.9e-61
Identity = 118/135 (87.41%), Postives = 127/135 (94.07%), Query Frame = 0


Query: 1   EVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEVRRIMAEIDKDGDGFIDLDEFAE 60
           EV  VF KFDKNGDGKIS+ EL  ALGELGGK+S DE+RRIM+EIDKDG+GFIDLDEFAE
Sbjct: 25  EVIMVFKKFDKNGDGKISIKELAAALGELGGKISDDEIRRIMSEIDKDGNGFIDLDEFAE 84

Query: 61  FHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV 120
           FHQ+ S+TGGG+KDLQDAFDLYD+DKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV
Sbjct: 85  FHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV 144

Query: 121 DGDGHVNFEEFKKMM 136
           DGDGHVNFEEFKKMM
Sbjct: 145 DGDGHVNFEEFKKMM 159

[Close]

BLAST of MS019144 vs. NCBI nr
Match: XP_022998716.1 (probable calcium-binding protein CML23 [Cucurbita maxima])

HSP 1 Score: 244.2 bits (622), Expect = 6.5e-61
Identity = 117/135 (86.67%), Postives = 127/135 (94.07%), Query Frame = 0


Query: 1   EVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEVRRIMAEIDKDGDGFIDLDEFAE 60
           EV  VF KFDKNGDGKIS+ EL  ALGELGGK+S DE+RRIM+EIDKDG+GFIDLDEFAE
Sbjct: 25  EVIMVFKKFDKNGDGKISIKELAAALGELGGKISDDEIRRIMSEIDKDGNGFIDLDEFAE 84

Query: 61  FHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV 120
           FHQ+ S+TGGG+KDLQDAFDL+D+DKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV
Sbjct: 85  FHQNFSSTGGGSKDLQDAFDLFDVDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV 144

Query: 121 DGDGHVNFEEFKKMM 136
           DGDGHVNFEEFKKMM
Sbjct: 145 DGDGHVNFEEFKKMM 159

[Close]

BLAST of MS019144 vs. NCBI nr
Match: XP_023526443.1 (calcium-binding protein CML24-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 242.7 bits (618), Expect = 1.9e-60
Identity = 117/135 (86.67%), Postives = 123/135 (91.11%), Query Frame = 0


Query: 1   EVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEVRRIMAEIDKDGDGFIDLDEFAE 60
           EV K+F KFDKNGDGKIS++ELG ALGELGGK+SSDE+RRIM EID DGDGFIDLDEF  
Sbjct: 17  EVMKIFKKFDKNGDGKISISELGAALGELGGKISSDEIRRIMLEIDTDGDGFIDLDEFTA 76

Query: 61  FHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV 120
           FHQD    GGGNKDLQDAFDLYD+DKNGLISAKELH VLKRLGEKCSLKDCCRMISSVDV
Sbjct: 77  FHQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEKCSLKDCCRMISSVDV 136

Query: 121 DGDGHVNFEEFKKMM 136
           DGDGHVNFEEFKKMM
Sbjct: 137 DGDGHVNFEEFKKMM 151

[Close]

BLAST of MS019144 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 2.9e-40
Identity = 81/137 (59.12%), Postives = 102/137 (74.45%), Query Frame = 0


Query: 1   EVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEVRRIMAEIDKDGDGFIDLDEFAE 60
           ++ KVF +FDKN DGKIS++EL D +G L    S +E + +M E D DG+GFIDLDEF  
Sbjct: 15  DIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVA 74

Query: 61  FHQ--DVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSV 120
             Q  D S+     +DL++AFDLYD+D+NG ISA ELHSV+K LGEKCS++DC RMI+ V
Sbjct: 75  LFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINKV 134

Query: 121 DVDGDGHVNFEEFKKMM 136
           D DGDG V+FEEFKKMM
Sbjct: 135 DSDGDGCVDFEEFKKMM 151

[Close]

BLAST of MS019144 vs. ExPASy Swiss-Prot
Match: P25070 (Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2)

HSP 1 Score: 162.2 bits (409), Expect = 4.2e-39
Identity = 82/140 (58.57%), Postives = 103/140 (73.57%), Query Frame = 0


Query: 1   EVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEVRRIMAEIDKDGDGFIDLDEFAE 60
           ++ KVF +FDKNGDGKISV+EL + +  L    S +E   +M + D DG+GFIDLDEF  
Sbjct: 17  DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76

Query: 61  FHQDVSATGGGN-----KDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMI 120
             Q +   GGGN      DL++AF+LYD+D NG ISAKELHSV+K LGEKCS++DC +MI
Sbjct: 77  LFQ-IGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMI 136

Query: 121 SSVDVDGDGHVNFEEFKKMM 136
           S VD+DGDG VNF+EFKKMM
Sbjct: 137 SKVDIDGDGCVNFDEFKKMM 155

[Close]

BLAST of MS019144 vs. ExPASy Swiss-Prot
Match: Q9LE22 (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 PE=1 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 2.6e-36
Identity = 76/135 (56.30%), Postives = 97/135 (71.85%), Query Frame = 0


Query: 1   EVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEVRRIMAEIDKDGDGFIDLDEFAE 60
           E+ KVF++FD NGDGKISV ELG     +G   +  E+ R++ E+D D DG+I+LDEF+ 
Sbjct: 23  ELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFST 82

Query: 61  FHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV 120
             +  S+      +++DAFDLYD DKNGLISA ELH VL RLG  CS++DC RMI  VD 
Sbjct: 83  LCRSSSSAA----EIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDA 142

Query: 121 DGDGHVNFEEFKKMM 136
           DGDG+VNFEEF+KMM
Sbjct: 143 DGDGNVNFEEFQKMM 153

[Close]

BLAST of MS019144 vs. ExPASy Swiss-Prot
Match: Q0DJV6 (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 GN=CML18 PE=2 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 4.4e-36
Identity = 78/147 (53.06%), Postives = 97/147 (65.99%), Query Frame = 0


Query: 1   EVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEVRRIMAEIDKDGDGFIDLDEFAE 60
           E+ +VF ++D NGDGKIS  EL   L  LG  +   EVRR+M E+D D DGF+DL EFA 
Sbjct: 5   ELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEFAA 64

Query: 61  FHQDVSATGGG------------NKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSL 120
           FH   +   GG              +L++AF +YD D NG ISA+ELH VL++LG+KCS+
Sbjct: 65  FHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKCSV 124

Query: 121 KDCCRMISSVDVDGDGHVNFEEFKKMM 136
            DC RMI SVD DGDG VNF+EFKKMM
Sbjct: 125 ADCSRMIRSVDADGDGCVNFDEFKKMM 151

[Close]

BLAST of MS019144 vs. ExPASy Swiss-Prot
Match: Q9M7R0 (Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 7.5e-36
Identity = 79/138 (57.25%), Postives = 96/138 (69.57%), Query Frame = 0


Query: 1   EVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEVRRIMAEIDKDGDGFIDLDEFAE 60
           EV  VFN+FD NGDGKIS +EL   L  LG   S +E+ RIM EID D DGFI++ EFA 
Sbjct: 20  EVQGVFNRFDANGDGKISGDELAGVLKALGSNTSKEEIGRIMEEIDTDKDGFINVQEFAA 79

Query: 61  F---HQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISS 120
           F     D   + GG  +L++AF+LYD D NGLIS+ ELH +L RLGE+ +  DC  MI S
Sbjct: 80  FVKAETDPYPSSGGENELKEAFELYDQDHNGLISSVELHKILTRLGERYAEHDCVEMIKS 139

Query: 121 VDVDGDGHVNFEEFKKMM 136
           VD DGDG+V+FEEFKKMM
Sbjct: 140 VDSDGDGYVSFEEFKKMM 157

[Close]

BLAST of MS019144 vs. ExPASy TrEMBL
Match: A0A6J1CNU4 (probable calcium-binding protein CML23 OS=Momordica charantia OX=3673 GN=LOC111013180 PE=4 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 4.8e-70
Identity = 135/135 (100.00%), Postives = 135/135 (100.00%), Query Frame = 0


Query: 1   EVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEVRRIMAEIDKDGDGFIDLDEFAE 60
           EVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEVRRIMAEIDKDGDGFIDLDEFAE
Sbjct: 25  EVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEVRRIMAEIDKDGDGFIDLDEFAE 84

Query: 61  FHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV 120
           FHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV
Sbjct: 85  FHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV 144

Query: 121 DGDGHVNFEEFKKMM 136
           DGDGHVNFEEFKKMM
Sbjct: 145 DGDGHVNFEEFKKMM 159

[Close]

BLAST of MS019144 vs. ExPASy TrEMBL
Match: A0A6J1G9H5 (probable calcium-binding protein CML23 OS=Cucurbita moschata OX=3662 GN=LOC111452166 PE=4 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 1.4e-61
Identity = 118/135 (87.41%), Postives = 127/135 (94.07%), Query Frame = 0


Query: 1   EVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEVRRIMAEIDKDGDGFIDLDEFAE 60
           EV  VF KFDKNGDGKIS+ EL  ALGELGGK+S DE+RRIM+EIDKDG+GFIDLDEFAE
Sbjct: 25  EVIMVFKKFDKNGDGKISIKELAAALGELGGKISDDEIRRIMSEIDKDGNGFIDLDEFAE 84

Query: 61  FHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV 120
           FHQ+ S+TGGG+KDLQDAFDLYD+DKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV
Sbjct: 85  FHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV 144

Query: 121 DGDGHVNFEEFKKMM 136
           DGDGHVNFEEFKKMM
Sbjct: 145 DGDGHVNFEEFKKMM 159

[Close]

BLAST of MS019144 vs. ExPASy TrEMBL
Match: A0A6J1KB05 (probable calcium-binding protein CML23 OS=Cucurbita maxima OX=3661 GN=LOC111493297 PE=4 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 3.1e-61
Identity = 117/135 (86.67%), Postives = 127/135 (94.07%), Query Frame = 0


Query: 1   EVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEVRRIMAEIDKDGDGFIDLDEFAE 60
           EV  VF KFDKNGDGKIS+ EL  ALGELGGK+S DE+RRIM+EIDKDG+GFIDLDEFAE
Sbjct: 25  EVIMVFKKFDKNGDGKISIKELAAALGELGGKISDDEIRRIMSEIDKDGNGFIDLDEFAE 84

Query: 61  FHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV 120
           FHQ+ S+TGGG+KDLQDAFDL+D+DKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV
Sbjct: 85  FHQNFSSTGGGSKDLQDAFDLFDVDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV 144

Query: 121 DGDGHVNFEEFKKMM 136
           DGDGHVNFEEFKKMM
Sbjct: 145 DGDGHVNFEEFKKMM 159

[Close]

BLAST of MS019144 vs. ExPASy TrEMBL
Match: A0A6J1F6R4 (calcium-binding protein CML24-like OS=Cucurbita moschata OX=3662 GN=LOC111441384 PE=4 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 2.0e-60
Identity = 116/135 (85.93%), Postives = 123/135 (91.11%), Query Frame = 0


Query: 1   EVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEVRRIMAEIDKDGDGFIDLDEFAE 60
           EV K+F KFDKNGDGKIS++ELG ALGELGG++SSDE+RRIM EID DGDGFIDLDEF  
Sbjct: 17  EVMKIFKKFDKNGDGKISISELGAALGELGGRISSDEIRRIMLEIDTDGDGFIDLDEFTA 76

Query: 61  FHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV 120
           FHQD    GGGNKDLQDAFDLYD+DKNGLISAKELH VLKRLGEKCSLKDCCRMISSVDV
Sbjct: 77  FHQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEKCSLKDCCRMISSVDV 136

Query: 121 DGDGHVNFEEFKKMM 136
           DGDGHVNFEEFKKMM
Sbjct: 137 DGDGHVNFEEFKKMM 151

[Close]

BLAST of MS019144 vs. ExPASy TrEMBL
Match: A0A6J1J811 (calcium-binding protein CML24-like OS=Cucurbita maxima OX=3661 GN=LOC111482109 PE=4 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 4.5e-60
Identity = 117/135 (86.67%), Postives = 123/135 (91.11%), Query Frame = 0


Query: 1   EVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEVRRIMAEIDKDGDGFIDLDEFAE 60
           EV K+F KFDKNGDGKISV+ELG ALGELGGK+SSDE RRIM EID DGDGFIDLDEFA 
Sbjct: 17  EVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLEIDTDGDGFIDLDEFAA 76

Query: 61  FHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV 120
           FH++    GGGNKDLQDAFDLYD+DKNGLISAKELH VLKRLGEKCSLKDCCRMISSVDV
Sbjct: 77  FHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEKCSLKDCCRMISSVDV 136

Query: 121 DGDGHVNFEEFKKMM 136
           DGDGHVNFEEFKKMM
Sbjct: 137 DGDGHVNFEEFKKMM 151

[Close]

BLAST of MS019144 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 166.0 bits (419), Expect = 2.1e-41
Identity = 81/137 (59.12%), Postives = 102/137 (74.45%), Query Frame = 0


Query: 1   EVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEVRRIMAEIDKDGDGFIDLDEFAE 60
           ++ KVF +FDKN DGKIS++EL D +G L    S +E + +M E D DG+GFIDLDEF  
Sbjct: 15  DIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVA 74

Query: 61  FHQ--DVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSV 120
             Q  D S+     +DL++AFDLYD+D+NG ISA ELHSV+K LGEKCS++DC RMI+ V
Sbjct: 75  LFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINKV 134

Query: 121 DVDGDGHVNFEEFKKMM 136
           D DGDG V+FEEFKKMM
Sbjct: 135 DSDGDGCVDFEEFKKMM 151

[Close]

BLAST of MS019144 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 162.2 bits (409), Expect = 3.0e-40
Identity = 82/140 (58.57%), Postives = 103/140 (73.57%), Query Frame = 0


Query: 1   EVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEVRRIMAEIDKDGDGFIDLDEFAE 60
           ++ KVF +FDKNGDGKISV+EL + +  L    S +E   +M + D DG+GFIDLDEF  
Sbjct: 17  DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76

Query: 61  FHQDVSATGGGN-----KDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMI 120
             Q +   GGGN      DL++AF+LYD+D NG ISAKELHSV+K LGEKCS++DC +MI
Sbjct: 77  LFQ-IGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMI 136

Query: 121 SSVDVDGDGHVNFEEFKKMM 136
           S VD+DGDG VNF+EFKKMM
Sbjct: 137 SKVDIDGDGCVNFDEFKKMM 155

[Close]

BLAST of MS019144 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 152.9 bits (385), Expect = 1.8e-37
Identity = 76/135 (56.30%), Postives = 97/135 (71.85%), Query Frame = 0


Query: 1   EVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEVRRIMAEIDKDGDGFIDLDEFAE 60
           E+ KVF++FD NGDGKISV ELG     +G   +  E+ R++ E+D D DG+I+LDEF+ 
Sbjct: 23  ELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFST 82

Query: 61  FHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV 120
             +  S+      +++DAFDLYD DKNGLISA ELH VL RLG  CS++DC RMI  VD 
Sbjct: 83  LCRSSSSAA----EIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDA 142

Query: 121 DGDGHVNFEEFKKMM 136
           DGDG+VNFEEF+KMM
Sbjct: 143 DGDGNVNFEEFQKMM 153

[Close]

BLAST of MS019144 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 152.9 bits (385), Expect = 1.8e-37
Identity = 76/135 (56.30%), Postives = 97/135 (71.85%), Query Frame = 0


Query: 1   EVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEVRRIMAEIDKDGDGFIDLDEFAE 60
           E+ KVF++FD NGDGKISV ELG     +G   +  E+ R++ E+D D DG+I+LDEF+ 
Sbjct: 23  ELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFST 82

Query: 61  FHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV 120
             +  S+      +++DAFDLYD DKNGLISA ELH VL RLG  CS++DC RMI  VD 
Sbjct: 83  LCRSSSSAA----EIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDA 142

Query: 121 DGDGHVNFEEFKKMM 136
           DGDG+VNFEEF+KMM
Sbjct: 143 DGDGNVNFEEFQKMM 153

[Close]

BLAST of MS019144 vs. TAIR 10
Match: AT1G73630.1 (EF hand calcium-binding protein family )

HSP 1 Score: 149.1 bits (375), Expect = 2.6e-36
Identity = 74/135 (54.81%), Postives = 99/135 (73.33%), Query Frame = 0


Query: 1   EVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEVRRIMAEIDKDGDGFIDLDEFAE 60
           E+ KVF+KFD NGDGKISV+ELG+    +G   + +E+ R++ EID D DGFI+ +EFA 
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEFAT 79

Query: 61  FHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV 120
             +  S+      ++++AFDLYD +KNGLIS+ E+H VL RLG  CS++DC RMI  VD 
Sbjct: 80  ICRSSSSA----VEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVDT 139

Query: 121 DGDGHVNFEEFKKMM 136
           DGDG+VNFEEF+KMM
Sbjct: 140 DGDGNVNFEEFQKMM 150

The following BLAST results are available for this feature:

BLAST of MS019144 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143269.1	9.9e-70	100.00	probable calcium-binding protein CML23 [Momordica charantia]	[more]
XP_038879983.1	4.0e-63	90.37	probable calcium-binding protein CML23 [Benincasa hispida]	[more]
XP_022948508.1	2.9e-61	87.41	probable calcium-binding protein CML23 [Cucurbita moschata] >XP_023524703.1 prob...	[more]
XP_022998716.1	6.5e-61	86.67	probable calcium-binding protein CML23 [Cucurbita maxima]	[more]
XP_023526443.1	1.9e-60	86.67	calcium-binding protein CML24-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS019144 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C8Y1	2.9e-40	59.12	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ...	[more]
P25070	4.2e-39	58.57	Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2	[more]
Q9LE22	2.6e-36	56.30	Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 ...	[more]
Q0DJV6	4.4e-36	53.06	Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9M7R0	7.5e-36	57.25	Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1	[more]

BLAST of MS019144 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CNU4	4.8e-70	100.00	probable calcium-binding protein CML23 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1G9H5	1.4e-61	87.41	probable calcium-binding protein CML23 OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]
A0A6J1KB05	3.1e-61	86.67	probable calcium-binding protein CML23 OS=Cucurbita maxima OX=3661 GN=LOC1114932...	[more]
A0A6J1F6R4	2.0e-60	85.93	calcium-binding protein CML24-like OS=Cucurbita moschata OX=3662 GN=LOC111441384...	[more]
A0A6J1J811	4.5e-60	86.67	calcium-binding protein CML24-like OS=Cucurbita maxima OX=3661 GN=LOC111482109 P...	[more]

BLAST of MS019144 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G66400.1	2.1e-41	59.12	calmodulin like 23	[more]
AT5G37770.1	3.0e-40	58.57	EF hand calcium-binding protein family	[more]
AT1G18210.1	1.8e-37	56.30	Calcium-binding EF-hand family protein	[more]
AT1G18210.2	1.8e-37	56.30	Calcium-binding EF-hand family protein	[more]
AT1G73630.1	2.6e-36	54.81	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 1..29 e-value: 1.9E-6 score: 37.4 coord: 74..102 e-value: 3.2E-5 score: 33.4 coord: 37..65 e-value: 2.4E-5 score: 33.8 coord: 110..135 e-value: 2.9 score: 14.1
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 4..61 e-value: 9.0E-15 score: 54.9 coord: 73..135 e-value: 1.5E-11 score: 44.6
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 1..32 score: 15.657116
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 106..135 score: 13.09074
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 33..68 score: 14.150764
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 70..105 score: 14.318138
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 1..63 e-value: 1.7408E-18 score: 71.8101
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 74..135 e-value: 6.40423E-18 score: 70.2693
None	No IPR available	GENE3D	1.10.238.10		coord: 71..135 e-value: 9.2E-22 score: 79.1
None	No IPR available	GENE3D	1.10.238.10		coord: 1..67 e-value: 2.7E-21 score: 77.2
None	No IPR available	PANTHER	PTHR10891:SF794	CALCIUM-BINDING PROTEIN CML23-RELATED	coord: 1..135
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 1..135
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 119..131
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 83..95
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 46..58
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 10..22
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 3..135

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS019144.1	MS019144.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding