MS019142 (gene) Bitter gourd (TR) v1

Overview
NameMS019142
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionmajor latex protein 15-like
Locationscaffold20: 1871308 .. 1872961 (+)
RNA-Seq ExpressionMS019142
SyntenyMS019142
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCAGATTGCTAACATCTCAGAGCAGGTGCAGCTCAAGTCTTGTGGCCACAAATTCTATGAGTTTCTAAAGAACAAAATGGACTACTTTCCAAGAATGTTCCCTGGAAACATTGAGAGCTATAAGTTTGTGGAAGGAAATAGTTTCACTCATGGCAGCATCTCCCACTGGAAATATGACATCGGTCTCGGTGAGATATCCTCCCACTTTGATTCCTAAACTTTCAAATATTTTTAATCCTTGAAGGCAGTTCTACTAGCAAGGACATGCAATCTCTAGGTAAAACACCTATCGCAGAGATCACATATTTGAACCTTAAAAGTTCTTAATGTCTCTCGGGTCGTGTTTTGAGGCAAGCACAGGTGCCTGGGATAGGAGGACTGAAGCTCAAATTCTGAAAAAACATGTTTTAATCTACACACCTATTGAAAATATGACATGAGTTCGTTTCGATTGATTAGATACCTCCTTACTTCATTTTAGATTAAAAAAATTATGTAAAAACATTTGAATTTTAAAATTTCCTCCCTAATTTGGTCATCATTCACTAAATTTTCATAAGTGAAATATTATACATATATTATACTTCACTTATTAAAATTTAGTATTTTATATATATATATATATATAAATAAAATACATAGANNNNNNNNNNNNNNNNNNNNNNNNNNAACTTTATCTTTTCATTTTATTTTTAATCATTTTTTATTATTATTTGGATGAAAATTTTGTAATTCATCTCTCCCATCTCTCTTCAAATTCTTTAAAATTCCACTAATTCTCTCTCTTTCTCTCCACTCCATTCTCTCTCGATTCCCCTCAAATTCCACTTCTCTCCCCCCTTAATTTTCAAATATATTTTCTCTACCTTTACAAATTCCACTTTTCTTAAATCTCTAAAAAAAAAACTTCATTTATCTCTCTCTTAATATTTTCATTTTTTTTCCATTCGCTACTCTTTTTTTTTCGCCNNNCGCCCATCCAAATACCTTACTCAAATTATCATTTATTAAGTTTATTTAAATTTTGTAAATATTGAGAGATTATTTAGATAAAAATATCGATATTTATATTTTTGAATGACAAAATTGGTCTTAAAGTAATAGTAATAAAATGATAAATTTCATGTACATCACACATGTTGCTCACTAATATAAATAAATTTATTCTTTCCCTCCATGTGAAAACTTTCGAATGTTTTAATAGTGATTCAGCTATTGATTTTGGTAGGAAAACACTCTCTCTCAGTCCTAAACTTTCAAGCGACGTTTCAACTTTCAAGCAGTTTATTTTCGTTCCTTCTTGCATCGTTAAATACGTATGTCTTTATATCAACTCAAATTATTGGGTTCGATTTTTGGTGGAAACAAACATATATACAGGTAGCTCAATCGAAGTAAAGATGAGGCTAATTGTGGATGAGCCTAACAAAACAATAATTTATGAGTGTCTTGAGGGAGATCTGTTCAAAGATTTTGAAATGTTCCAAGTGAAAATTGAAGTTAGCGATGGTGAAGGCAACAATGGCATAAGCTCAGTTAAATGGTCTTTGGAATTTGTGAAGGCAAATGAAGAAGTTCCTCCACCACATGACTATCTACAACTTGGAGTTAAAGTGTGCAAAAGTGTGGATGCTCTCCTTTGCAACAAT

mRNA sequence

ATGGCTCAGATTGCTAACATCTCAGAGCAGGTGCAGCTCAAGTCTTGTGGCCACAAATTCTATGAGTTTCTAAAGAACAAAATGGACTACTTTCCAAGAATGTTCCCTGGAAACATTGAGAGCTATAAGTTTGTGGAAGGAAATAGTTTCACTCATGGCAGCATCTCCCACTGGAAATATGACATCGGTCTCGGTAGCTCAATCGAAGTAAAGATGAGGCTAATTGTGGATGAGCCTAACAAAACAATAATTTATGAGTGTCTTGAGGGAGATCTGTTCAAAGATTTTGAAATGTTCCAAGTGAAAATTGAAGTTAGCGATGGTGAAGGCAACAATGGCATAAGCTCAGTTAAATGGTCTTTGGAATTTGTGAAGGCAAATGAAGAAGTTCCTCCACCACATGACTATCTACAACTTGGAGTTAAAGTGTGCAAAAGTGTGGATGCTCTCCTTTGCAACAAT

Coding sequence (CDS)

ATGGCTCAGATTGCTAACATCTCAGAGCAGGTGCAGCTCAAGTCTTGTGGCCACAAATTCTATGAGTTTCTAAAGAACAAAATGGACTACTTTCCAAGAATGTTCCCTGGAAACATTGAGAGCTATAAGTTTGTGGAAGGAAATAGTTTCACTCATGGCAGCATCTCCCACTGGAAATATGACATCGGTCTCGGTAGCTCAATCGAAGTAAAGATGAGGCTAATTGTGGATGAGCCTAACAAAACAATAATTTATGAGTGTCTTGAGGGAGATCTGTTCAAAGATTTTGAAATGTTCCAAGTGAAAATTGAAGTTAGCGATGGTGAAGGCAACAATGGCATAAGCTCAGTTAAATGGTCTTTGGAATTTGTGAAGGCAAATGAAGAAGTTCCTCCACCACATGACTATCTACAACTTGGAGTTAAAGTGTGCAAAAGTGTGGATGCTCTCCTTTGCAACAAT

Protein sequence

MAQIANISEQVQLKSCGHKFYEFLKNKMDYFPRMFPGNIESYKFVEGNSFTHGSISHWKYDIGLGSSIEVKMRLIVDEPNKTIIYECLEGDLFKDFEMFQVKIEVSDGEGNNGISSVKWSLEFVKANEEVPPPHDYLQLGVKVCKSVDALLCNN
Homology
BLAST of MS019142 vs. NCBI nr
Match: XP_022143420.1 (uncharacterized protein LOC111013301 [Momordica charantia])

HSP 1 Score: 320.1 bits (819), Expect = 1.1e-83
Identity = 152/154 (98.70%), Postives = 152/154 (98.70%), Query Frame = 0

Query: 1   MAQIANISEQVQLKSCGHKFYEFLKNKMDYFPRMFPGNIESYKFVEGNSFTHGSISHWKY 60
           MAQIA ISEQVQLKSCGHKFYEFLKNKMDYFPRMFPGNIESYKF EGNSFTHGSISHWKY
Sbjct: 1   MAQIAKISEQVQLKSCGHKFYEFLKNKMDYFPRMFPGNIESYKFAEGNSFTHGSISHWKY 60

Query: 61  DIGLGSSIEVKMRLIVDEPNKTIIYECLEGDLFKDFEMFQVKIEVSDGEGNNGISSVKWS 120
           DIGLGSSIEVKMRLIVDEPNKTIIYECLEGDLFKDFEMFQVKIEVSDGEGNNGISSVKWS
Sbjct: 61  DIGLGSSIEVKMRLIVDEPNKTIIYECLEGDLFKDFEMFQVKIEVSDGEGNNGISSVKWS 120

Query: 121 LEFVKANEEVPPPHDYLQLGVKVCKSVDALLCNN 155
           LEFVKANEEVPPPHDYLQLGVKVCKSVDALLCNN
Sbjct: 121 LEFVKANEEVPPPHDYLQLGVKVCKSVDALLCNN 154

BLAST of MS019142 vs. NCBI nr
Match: XP_038880356.1 (MLP-like protein 34 [Benincasa hispida])

HSP 1 Score: 258.8 bits (660), Expect = 2.9e-65
Identity = 121/152 (79.61%), Postives = 134/152 (88.16%), Query Frame = 0

Query: 1   MAQIANISEQVQLKSCGHKFYEFLKNKMDYFPRMFPGNIESYKFVEGNSFTHGSISHWKY 60
           MAQIA ISE+VQLKS G +FY+F KNKMDYFPRMFPGN+ESYKFVEGNSFTHGS+S WKY
Sbjct: 1   MAQIAKISEKVQLKSSGKRFYQFFKNKMDYFPRMFPGNVESYKFVEGNSFTHGSVSVWKY 60

Query: 61  DIGLGSSIEVKMRLIVDEPNKTIIYECLEGDLFKDFEMFQVKIEVSDGEGNNGISSVKWS 120
           D G GSS+EVK++L+VDEPNKTIIYECLEGDLFKDFEMF VKIEV+DG GNNG SSV W 
Sbjct: 61  DFGFGSSVEVKVKLLVDEPNKTIIYECLEGDLFKDFEMFNVKIEVNDG-GNNGNSSVNWC 120

Query: 121 LEFVKANEEVPPPHDYLQLGVKVCKSVDALLC 153
           +EFVKANE V PP+ YLQ GVK+CK VDA LC
Sbjct: 121 VEFVKANENVAPPNSYLQFGVKICKDVDAHLC 151

BLAST of MS019142 vs. NCBI nr
Match: XP_004145832.1 (MLP-like protein 34 [Cucumis sativus])

HSP 1 Score: 252.3 bits (643), Expect = 2.7e-63
Identity = 118/154 (76.62%), Postives = 135/154 (87.66%), Query Frame = 0

Query: 1   MAQIANISEQVQLKSCGHKFYEFLKNKMDYFPRMFPGNIESYKFVEGNSFTHGSISHWKY 60
           MAQIA I+E+VQLKS G KF+EF KNKMDYFPRMF GN+ESYKFVEGNSFTHGS+S WKY
Sbjct: 1   MAQIAKIAEKVQLKSSGEKFFEFFKNKMDYFPRMFAGNVESYKFVEGNSFTHGSVSIWKY 60

Query: 61  DIGLGSSIEVKMRLIVDEPNKTIIYECLEGDLFKDFEMFQVKIEVSDGEGNNGISSVKWS 120
           DIG G ++EVKM+L+VDE NKTIIYECLEGDLFKDF+MF+VKIEV+DG G++G SSV W 
Sbjct: 61  DIGFGRAVEVKMKLLVDEANKTIIYECLEGDLFKDFDMFKVKIEVTDG-GSSGNSSVNWC 120

Query: 121 LEFVKANEEVPPPHDYLQLGVKVCKSVDALLCNN 155
           LEFVK+NE V PP+DYLQ GVK+CK VDA L NN
Sbjct: 121 LEFVKSNENVAPPNDYLQFGVKICKDVDAYLSNN 153

BLAST of MS019142 vs. NCBI nr
Match: KAE8650605.1 (hypothetical protein Csa_010824 [Cucumis sativus])

HSP 1 Score: 252.3 bits (643), Expect = 2.7e-63
Identity = 118/154 (76.62%), Postives = 135/154 (87.66%), Query Frame = 0

Query: 1   MAQIANISEQVQLKSCGHKFYEFLKNKMDYFPRMFPGNIESYKFVEGNSFTHGSISHWKY 60
           MAQIA I+E+VQLKS G KF+EF KNKMDYFPRMF GN+ESYKFVEGNSFTHGS+S WKY
Sbjct: 38  MAQIAKIAEKVQLKSSGEKFFEFFKNKMDYFPRMFAGNVESYKFVEGNSFTHGSVSIWKY 97

Query: 61  DIGLGSSIEVKMRLIVDEPNKTIIYECLEGDLFKDFEMFQVKIEVSDGEGNNGISSVKWS 120
           DIG G ++EVKM+L+VDE NKTIIYECLEGDLFKDF+MF+VKIEV+DG G++G SSV W 
Sbjct: 98  DIGFGRAVEVKMKLLVDEANKTIIYECLEGDLFKDFDMFKVKIEVTDG-GSSGNSSVNWC 157

Query: 121 LEFVKANEEVPPPHDYLQLGVKVCKSVDALLCNN 155
           LEFVK+NE V PP+DYLQ GVK+CK VDA L NN
Sbjct: 158 LEFVKSNENVAPPNDYLQFGVKICKDVDAYLSNN 190

BLAST of MS019142 vs. NCBI nr
Match: XP_008465556.1 (PREDICTED: MLP-like protein 28 [Cucumis melo] >KAA0053134.1 MLP-like protein 28 [Cucumis melo var. makuwa] >TYK01540.1 MLP-like protein 28 [Cucumis melo var. makuwa])

HSP 1 Score: 249.2 bits (635), Expect = 2.3e-62
Identity = 114/154 (74.03%), Postives = 135/154 (87.66%), Query Frame = 0

Query: 1   MAQIANISEQVQLKSCGHKFYEFLKNKMDYFPRMFPGNIESYKFVEGNSFTHGSISHWKY 60
           MAQIA I+E+VQLKS G KF+EF KNK DYFPRMFPGNI+SYKFVEGNSF+HGS+S WKY
Sbjct: 1   MAQIAKIAEKVQLKSSGEKFFEFFKNKADYFPRMFPGNIKSYKFVEGNSFSHGSVSIWKY 60

Query: 61  DIGLGSSIEVKMRLIVDEPNKTIIYECLEGDLFKDFEMFQVKIEVSDGEGNNGISSVKWS 120
           D+G G ++EVK++L++DE NKTIIYECLEGDLFKDF+MF+VKIEV+DG G++G SSV W 
Sbjct: 61  DLGFGRAVEVKVKLLLDEANKTIIYECLEGDLFKDFDMFKVKIEVTDG-GSSGNSSVNWC 120

Query: 121 LEFVKANEEVPPPHDYLQLGVKVCKSVDALLCNN 155
           LEFVK+NE V PP+DYLQ G K+CK VDA LCNN
Sbjct: 121 LEFVKSNENVAPPNDYLQFGAKICKDVDAYLCNN 153

BLAST of MS019142 vs. ExPASy Swiss-Prot
Match: Q9SSK7 (MLP-like protein 34 OS=Arabidopsis thaliana OX=3702 GN=MLP34 PE=2 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 2.3e-17
Identity = 50/143 (34.97%), Postives = 77/143 (53.85%), Query Frame = 0

Query: 7   ISEQVQLKSCGHKFYEFLKNKMDYFPRMFPGNIESYKFVEGNSFTHGSISHWKYDIGLGS 66
           +  +V++K+   KF+     K  +  +  PGNI+S    EG+  T GSI  W Y +  G 
Sbjct: 169 LETEVEIKASAEKFHHMFAGKPHHVSKATPGNIQSCDLHEGDWGTVGSIVFWNY-VHDGE 228

Query: 67  SIEVKMRLIVDEPNKTII-YECLEGDLFKDFEMFQVKIEVSDGEGNNGISSVKWSLEFVK 126
           +   K R+   +P K +I +  +EGDL K+++ F + I+V+   G +G S V W  E+ K
Sbjct: 229 AKVAKERIEAVDPEKNLITFRVIEGDLMKEYKSFVITIQVTPKHGGSG-SVVHWHFEYEK 288

Query: 127 ANEEVPPPHDYLQLGVKVCKSVD 149
            NEEV  P   LQ  V+V K +D
Sbjct: 289 INEEVAHPETLLQFAVEVSKEID 309

BLAST of MS019142 vs. ExPASy Swiss-Prot
Match: Q941R6 (MLP-like protein 31 OS=Arabidopsis thaliana OX=3702 GN=MLP31 PE=2 SV=2)

HSP 1 Score: 77.8 bits (190), Expect = 1.2e-13
Identity = 44/148 (29.73%), Postives = 76/148 (51.35%), Query Frame = 0

Query: 7   ISEQVQLKSCGHKFYEFLKNKMDYFPRMFPGNIESYKFVEGNSFTHGSISHWKYDIGLGS 66
           +   +++K+   KF+     +  +  +  PG I+  +  EG+    GSI  W Y +  G 
Sbjct: 24  LETDIEIKASAGKFHHMFAGRPHHVSKATPGKIQGCELHEGDWGKVGSIVFWNY-VHDGE 83

Query: 67  SIEVKMRLIVDEPNKTII-YECLEGDLFKDFEMFQVKIEVSDGEGNNGISSVKWSLEFVK 126
           +   K R+   EP K +I +  +EGDL K+++ F + I+V+   G  G S V W +E+ K
Sbjct: 84  AKVAKERIEAVEPEKNLITFRVIEGDLLKEYKSFVITIQVTPKRGGPG-SVVHWHVEYEK 143

Query: 127 ANEEVPPPHDYLQLGVKVCKSVDALLCN 154
            +++V  P  +L   V+V K +D  L N
Sbjct: 144 IDDKVAHPETFLDFCVEVSKEIDEHLLN 169

BLAST of MS019142 vs. ExPASy Swiss-Prot
Match: Q06395 (Major latex protein 149 OS=Papaver somniferum OX=3469 GN=MLP149 PE=2 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 2.9e-12
Identity = 41/149 (27.52%), Postives = 73/149 (48.99%), Query Frame = 0

Query: 4   IANISEQVQLKSCGHKFYEFLKNKMDYFPRMFPGNIESYKFVEGNSFTHGSISHWKYDIG 63
           +  +  + ++     K+Y+  K+  D  P   P  + S K VEG+  T G +  W Y + 
Sbjct: 11  VGKLVMETEVNCNADKYYQIYKHHED-LPSAIPHIVTSAKAVEGHGTTSGCVKEWGY-MH 70

Query: 64  LGSSIEVKMRLIVDEPNKTIIYECLEGDLFKDFEMFQVKIEVSDGEGNNGISSVKWSLEF 123
            G ++  K +   ++  +TI +   EGDL  D++ F   + V   +  +G S VK+ L++
Sbjct: 71  EGKTLTCKEKTTYNDETRTICHSISEGDLMNDYKKFDATLVVDPKDNGHG-SIVKYILDY 130

Query: 124 VKANEEVPPPHDYLQLGVKVCKSVDALLC 153
            K NE+ P P  YL L  +  + ++  LC
Sbjct: 131 EKINEDSPVPIHYLALCNQATEDLNTYLC 156

BLAST of MS019142 vs. ExPASy Swiss-Prot
Match: P85524 (Kirola OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 6.6e-12
Identity = 39/140 (27.86%), Postives = 75/140 (53.57%), Query Frame = 0

Query: 9   EQVQLKSCGHKFYEFLKNKMDYFPRMFPGNIESYKFVEGNSFTHGSISHWKYDIGLGSSI 68
           +QV++ S G  FYE  + ++     M P NI+    +EGN  T GS+  +KY I      
Sbjct: 9   KQVEILSDGIVFYEIFRYRLYLISEMSPVNIQGVDLLEGNWGTVGSVIFFKYTIDGKEKT 68

Query: 69  EVKMRLIVDEPNKTIIYECLEGDLFKDFEMFQVKIEVSDGEGNNGISSVKWSLEFVKANE 128
              +   +DE  K++ ++ +EGDL + ++ F + ++V D +G +  +SV W+  + K  E
Sbjct: 69  AKDIVEAIDEETKSVTFKIVEGDLMELYKTFIIIVQV-DTKGEH--NSVTWTFHYEKLKE 128

Query: 129 EVPPPHDYLQLGVKVCKSVD 149
           +V  P+  +   +++ K ++
Sbjct: 129 DVEEPNTLMNFCIEITKDIE 145

BLAST of MS019142 vs. ExPASy Swiss-Prot
Match: Q06394 (Major latex protein 146 OS=Papaver somniferum OX=3469 GN=MLP146 PE=2 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 3.3e-11
Identity = 39/134 (29.10%), Postives = 67/134 (50.00%), Query Frame = 0

Query: 19  KFYEFLKNKMDYFPRMFPGNIESYKFVEGNSFTHGSISHWKYDIGLGSSIEVKMRLIVDE 78
           K+Y+  K+  D  P + P    S K VEG+  T G +  W Y I  G  +  K +   ++
Sbjct: 26  KYYKLYKHHED-LPSVIPHIYTSVKAVEGHGTTSGCVKEWGY-ILEGKPLSCKEKTTYND 85

Query: 79  PNKTIIYECLEGDLFKDFEMFQVKIEVSDGEGNNGISSVKWSLEFVKANEEVPPPHDYLQ 138
             +TI +  + GDL  D++ F   + V+     +G   VKW++++ K NE+ P P  YL 
Sbjct: 86  ETRTIHHMVVAGDLMNDYKKFDATLVVNPKSNGHG-CIVKWTIDYEKMNEDSPVPFGYLA 145

Query: 139 LGVKVCKSVDALLC 153
              ++ + + + LC
Sbjct: 146 CYQQITEDLSSHLC 156

BLAST of MS019142 vs. ExPASy TrEMBL
Match: A0A6J1CNR9 (uncharacterized protein LOC111013301 OS=Momordica charantia OX=3673 GN=LOC111013301 PE=3 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 5.1e-84
Identity = 152/154 (98.70%), Postives = 152/154 (98.70%), Query Frame = 0

Query: 1   MAQIANISEQVQLKSCGHKFYEFLKNKMDYFPRMFPGNIESYKFVEGNSFTHGSISHWKY 60
           MAQIA ISEQVQLKSCGHKFYEFLKNKMDYFPRMFPGNIESYKF EGNSFTHGSISHWKY
Sbjct: 1   MAQIAKISEQVQLKSCGHKFYEFLKNKMDYFPRMFPGNIESYKFAEGNSFTHGSISHWKY 60

Query: 61  DIGLGSSIEVKMRLIVDEPNKTIIYECLEGDLFKDFEMFQVKIEVSDGEGNNGISSVKWS 120
           DIGLGSSIEVKMRLIVDEPNKTIIYECLEGDLFKDFEMFQVKIEVSDGEGNNGISSVKWS
Sbjct: 61  DIGLGSSIEVKMRLIVDEPNKTIIYECLEGDLFKDFEMFQVKIEVSDGEGNNGISSVKWS 120

Query: 121 LEFVKANEEVPPPHDYLQLGVKVCKSVDALLCNN 155
           LEFVKANEEVPPPHDYLQLGVKVCKSVDALLCNN
Sbjct: 121 LEFVKANEEVPPPHDYLQLGVKVCKSVDALLCNN 154

BLAST of MS019142 vs. ExPASy TrEMBL
Match: A0A5A7UCZ9 (MLP-like protein 28 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G001090 PE=3 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 1.1e-62
Identity = 114/154 (74.03%), Postives = 135/154 (87.66%), Query Frame = 0

Query: 1   MAQIANISEQVQLKSCGHKFYEFLKNKMDYFPRMFPGNIESYKFVEGNSFTHGSISHWKY 60
           MAQIA I+E+VQLKS G KF+EF KNK DYFPRMFPGNI+SYKFVEGNSF+HGS+S WKY
Sbjct: 1   MAQIAKIAEKVQLKSSGEKFFEFFKNKADYFPRMFPGNIKSYKFVEGNSFSHGSVSIWKY 60

Query: 61  DIGLGSSIEVKMRLIVDEPNKTIIYECLEGDLFKDFEMFQVKIEVSDGEGNNGISSVKWS 120
           D+G G ++EVK++L++DE NKTIIYECLEGDLFKDF+MF+VKIEV+DG G++G SSV W 
Sbjct: 61  DLGFGRAVEVKVKLLLDEANKTIIYECLEGDLFKDFDMFKVKIEVTDG-GSSGNSSVNWC 120

Query: 121 LEFVKANEEVPPPHDYLQLGVKVCKSVDALLCNN 155
           LEFVK+NE V PP+DYLQ G K+CK VDA LCNN
Sbjct: 121 LEFVKSNENVAPPNDYLQFGAKICKDVDAYLCNN 153

BLAST of MS019142 vs. ExPASy TrEMBL
Match: A0A1S3CQL5 (MLP-like protein 28 OS=Cucumis melo OX=3656 GN=LOC103503185 PE=3 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 1.1e-62
Identity = 114/154 (74.03%), Postives = 135/154 (87.66%), Query Frame = 0

Query: 1   MAQIANISEQVQLKSCGHKFYEFLKNKMDYFPRMFPGNIESYKFVEGNSFTHGSISHWKY 60
           MAQIA I+E+VQLKS G KF+EF KNK DYFPRMFPGNI+SYKFVEGNSF+HGS+S WKY
Sbjct: 1   MAQIAKIAEKVQLKSSGEKFFEFFKNKADYFPRMFPGNIKSYKFVEGNSFSHGSVSIWKY 60

Query: 61  DIGLGSSIEVKMRLIVDEPNKTIIYECLEGDLFKDFEMFQVKIEVSDGEGNNGISSVKWS 120
           D+G G ++EVK++L++DE NKTIIYECLEGDLFKDF+MF+VKIEV+DG G++G SSV W 
Sbjct: 61  DLGFGRAVEVKVKLLLDEANKTIIYECLEGDLFKDFDMFKVKIEVTDG-GSSGNSSVNWC 120

Query: 121 LEFVKANEEVPPPHDYLQLGVKVCKSVDALLCNN 155
           LEFVK+NE V PP+DYLQ G K+CK VDA LCNN
Sbjct: 121 LEFVKSNENVAPPNDYLQFGAKICKDVDAYLCNN 153

BLAST of MS019142 vs. ExPASy TrEMBL
Match: A0A0A0L7N6 (Bet_v_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G331330 PE=3 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 1.4e-60
Identity = 113/154 (73.38%), Postives = 131/154 (85.06%), Query Frame = 0

Query: 1   MAQIANISEQVQLKSCGHKFYEFLKNKMDYFPRMFPGNIESYKFVEGNSFTHGSISHWKY 60
           MAQIA IS+QVQLK CG KFY+F KNKMD+ PR+FP   ESYK VEGNS THGS+S WKY
Sbjct: 1   MAQIAKISDQVQLKCCGKKFYDFFKNKMDHLPRVFPQYFESYKLVEGNSLTHGSVSFWKY 60

Query: 61  DIGLGSSIEVKMRLIVDEPNKTIIYECLEGDLFKDFEMFQVKIEVSDGEGNNGISSVKWS 120
           D G GSSIEVKM+++VDEPNKTIIYECLEGDLFKDF++F VKI+V+DG GNNG SSV W 
Sbjct: 61  DFGFGSSIEVKMKVLVDEPNKTIIYECLEGDLFKDFDIFNVKIKVNDG-GNNGNSSVNWC 120

Query: 121 LEFVKANEEVPPPHDYLQLGVKVCKSVDALLCNN 155
           LE+VKANE V PP++YLQ G+K+CK+VDA L NN
Sbjct: 121 LEYVKANENVDPPNNYLQFGLKLCKNVDAFLGNN 153

BLAST of MS019142 vs. ExPASy TrEMBL
Match: A0A1S3C5B3 (major latex protein 15-like OS=Cucumis melo OX=3656 GN=LOC103496826 PE=3 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 1.7e-55
Identity = 103/151 (68.21%), Postives = 125/151 (82.78%), Query Frame = 0

Query: 1   MAQIANISEQVQLKSCGHKFYEFLKNKMDYFPRMFPGNIESYKFVEGNSFTHGSISHWKY 60
           MAQI NISE+V+L  CG KFY+F  NKMD +  +FP N++SYKFVEGN FTHGS++HWKY
Sbjct: 1   MAQICNISEEVKLNCCGDKFYDFFLNKMDSYIHLFPQNLQSYKFVEGNGFTHGSVTHWKY 60

Query: 61  DIGLGSSIEVKMRLIVDEPNKTIIYECLEGDLFKDFEMFQVKIEVSDGEGNNGISSVKWS 120
           D G+ +  EVK RL+VDEPNK +I+EC+EGDL KDFEMFQVK+EV DG G NG+SSVKWS
Sbjct: 61  DFGIPA--EVKTRLLVDEPNKAVIFECVEGDLLKDFEMFQVKVEVRDG-GKNGVSSVKWS 120

Query: 121 LEFVKANEEVPPPHDYLQLGVKVCKSVDALL 152
           + F+KANE+V PPH+YLQ GVKVCK +DA L
Sbjct: 121 VGFMKANEDVAPPHNYLQFGVKVCKGLDAYL 148

BLAST of MS019142 vs. TAIR 10
Match: AT1G70850.1 (MLP-like protein 34 )

HSP 1 Score: 90.1 bits (222), Expect = 1.7e-18
Identity = 50/143 (34.97%), Postives = 77/143 (53.85%), Query Frame = 0

Query: 7   ISEQVQLKSCGHKFYEFLKNKMDYFPRMFPGNIESYKFVEGNSFTHGSISHWKYDIGLGS 66
           +  +V++K+   KF+     K  +  +  PGNI+S    EG+  T GSI  W Y +  G 
Sbjct: 169 LETEVEIKASAEKFHHMFAGKPHHVSKATPGNIQSCDLHEGDWGTVGSIVFWNY-VHDGE 228

Query: 67  SIEVKMRLIVDEPNKTII-YECLEGDLFKDFEMFQVKIEVSDGEGNNGISSVKWSLEFVK 126
           +   K R+   +P K +I +  +EGDL K+++ F + I+V+   G +G S V W  E+ K
Sbjct: 229 AKVAKERIEAVDPEKNLITFRVIEGDLMKEYKSFVITIQVTPKHGGSG-SVVHWHFEYEK 288

Query: 127 ANEEVPPPHDYLQLGVKVCKSVD 149
            NEEV  P   LQ  V+V K +D
Sbjct: 289 INEEVAHPETLLQFAVEVSKEID 309

BLAST of MS019142 vs. TAIR 10
Match: AT1G70850.3 (MLP-like protein 34 )

HSP 1 Score: 90.1 bits (222), Expect = 1.7e-18
Identity = 50/143 (34.97%), Postives = 77/143 (53.85%), Query Frame = 0

Query: 7   ISEQVQLKSCGHKFYEFLKNKMDYFPRMFPGNIESYKFVEGNSFTHGSISHWKYDIGLGS 66
           +  +V++K+   KF+     K  +  +  PGNI+S    EG+  T GSI  W Y +  G 
Sbjct: 169 LETEVEIKASAEKFHHMFAGKPHHVSKATPGNIQSCDLHEGDWGTVGSIVFWNY-VHDGE 228

Query: 67  SIEVKMRLIVDEPNKTII-YECLEGDLFKDFEMFQVKIEVSDGEGNNGISSVKWSLEFVK 126
           +   K R+   +P K +I +  +EGDL K+++ F + I+V+   G +G S V W  E+ K
Sbjct: 229 AKVAKERIEAVDPEKNLITFRVIEGDLMKEYKSFVITIQVTPKHGGSG-SVVHWHFEYEK 288

Query: 127 ANEEVPPPHDYLQLGVKVCKSVD 149
            NEEV  P   LQ  V+V K +D
Sbjct: 289 INEEVAHPETLLQFAVEVSKEID 309

BLAST of MS019142 vs. TAIR 10
Match: AT5G28010.1 (Polyketide cyclase/dehydrase and lipid transport superfamily protein )

HSP 1 Score: 79.0 bits (193), Expect = 3.8e-15
Identity = 43/146 (29.45%), Postives = 77/146 (52.74%), Query Frame = 0

Query: 4   IANISEQVQLKSCGHKFYEFLKNKMDYFPRMFPGNIESYKFVEGNSFTHGSISHWKYDIG 63
           +  +  +V++K+    FY     +  +  +  P N++S    +G   T GSI +W Y + 
Sbjct: 16  LGKLEVEVEIKAPAAIFYHIYAGRPHHVAKATPRNVQSCDLHDGEWGTVGSIVYWNY-VH 75

Query: 64  LGSSIEVKMRLIVDEPNKTII-YECLEGDLFKDFEMFQVKIEVSDGEGNNGISSVKWSLE 123
            G +   K R+ + EP K +I +  +EGD+  +++ F + I+V+  EG  G S VKW +E
Sbjct: 76  EGQAKVAKERIELVEPEKKLIKFRVIEGDVMAEYKSFLITIQVTPKEGGTG-SVVKWHIE 135

Query: 124 FVKANEEVPPPHDYLQLGVKVCKSVD 149
           + K +E VP P + L    ++ K +D
Sbjct: 136 YEKIDENVPHPENLLPFFAEMTKEID 159

BLAST of MS019142 vs. TAIR 10
Match: AT1G70840.1 (MLP-like protein 31 )

HSP 1 Score: 77.8 bits (190), Expect = 8.5e-15
Identity = 44/148 (29.73%), Postives = 76/148 (51.35%), Query Frame = 0

Query: 7   ISEQVQLKSCGHKFYEFLKNKMDYFPRMFPGNIESYKFVEGNSFTHGSISHWKYDIGLGS 66
           +   +++K+   KF+     +  +  +  PG I+  +  EG+    GSI  W Y +  G 
Sbjct: 24  LETDIEIKASAGKFHHMFAGRPHHVSKATPGKIQGCELHEGDWGKVGSIVFWNY-VHDGE 83

Query: 67  SIEVKMRLIVDEPNKTII-YECLEGDLFKDFEMFQVKIEVSDGEGNNGISSVKWSLEFVK 126
           +   K R+   EP K +I +  +EGDL K+++ F + I+V+   G  G S V W +E+ K
Sbjct: 84  AKVAKERIEAVEPEKNLITFRVIEGDLLKEYKSFVITIQVTPKRGGPG-SVVHWHVEYEK 143

Query: 127 ANEEVPPPHDYLQLGVKVCKSVDALLCN 154
            +++V  P  +L   V+V K +D  L N
Sbjct: 144 IDDKVAHPETFLDFCVEVSKEIDEHLLN 169

BLAST of MS019142 vs. TAIR 10
Match: AT5G28000.1 (Polyketide cyclase/dehydrase and lipid transport superfamily protein )

HSP 1 Score: 73.2 bits (178), Expect = 2.1e-13
Identity = 42/143 (29.37%), Postives = 73/143 (51.05%), Query Frame = 0

Query: 7   ISEQVQLKSCGHKFYEFLKNKMDYFPRMFPGNIESYKFVEGNSFTHGSISHWKYDIGLGS 66
           +  +V +KS   KFY+    + D+  +     +++   +EG   T  SI +W Y +  G 
Sbjct: 17  VEVEVDIKSPAEKFYQVYVGRPDHVAKATSSKVQACDLLEGEWGTISSIVNWNY-VYAGK 76

Query: 67  SIEVKMRLIVDEPNKTII-YECLEGDLFKDFEMFQVKIEVSDGEGNNGISSVKWSLEFVK 126
           +   K R+ + EP K +I +  +EGD+   ++ F + I V+  EG  G S  KW LE+ K
Sbjct: 77  AKVAKERIELVEPEKKLIKFRVIEGDVLAVYKNFFITISVTPKEGGVG-SVAKWHLEYEK 136

Query: 127 ANEEVPPPHDYLQLGVKVCKSVD 149
            +  VP P ++L    ++ K +D
Sbjct: 137 NDVNVPDPENFLPFLAEMTKEID 157

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022143420.11.1e-8398.70uncharacterized protein LOC111013301 [Momordica charantia][more]
XP_038880356.12.9e-6579.61MLP-like protein 34 [Benincasa hispida][more]
XP_004145832.12.7e-6376.62MLP-like protein 34 [Cucumis sativus][more]
KAE8650605.12.7e-6376.62hypothetical protein Csa_010824 [Cucumis sativus][more]
XP_008465556.12.3e-6274.03PREDICTED: MLP-like protein 28 [Cucumis melo] >KAA0053134.1 MLP-like protein 28 ... [more]
Match NameE-valueIdentityDescription
Q9SSK72.3e-1734.97MLP-like protein 34 OS=Arabidopsis thaliana OX=3702 GN=MLP34 PE=2 SV=1[more]
Q941R61.2e-1329.73MLP-like protein 31 OS=Arabidopsis thaliana OX=3702 GN=MLP31 PE=2 SV=2[more]
Q063952.9e-1227.52Major latex protein 149 OS=Papaver somniferum OX=3469 GN=MLP149 PE=2 SV=1[more]
P855246.6e-1227.86Kirola OS=Actinidia deliciosa OX=3627 PE=1 SV=1[more]
Q063943.3e-1129.10Major latex protein 146 OS=Papaver somniferum OX=3469 GN=MLP146 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CNR95.1e-8498.70uncharacterized protein LOC111013301 OS=Momordica charantia OX=3673 GN=LOC111013... [more]
A0A5A7UCZ91.1e-6274.03MLP-like protein 28 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G... [more]
A0A1S3CQL51.1e-6274.03MLP-like protein 28 OS=Cucumis melo OX=3656 GN=LOC103503185 PE=3 SV=1[more]
A0A0A0L7N61.4e-6073.38Bet_v_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G331330 PE=... [more]
A0A1S3C5B31.7e-5568.21major latex protein 15-like OS=Cucumis melo OX=3656 GN=LOC103496826 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G70850.11.7e-1834.97MLP-like protein 34 [more]
AT1G70850.31.7e-1834.97MLP-like protein 34 [more]
AT5G28010.13.8e-1529.45Polyketide cyclase/dehydrase and lipid transport superfamily protein [more]
AT1G70840.18.5e-1529.73MLP-like protein 31 [more]
AT5G28000.12.1e-1329.37Polyketide cyclase/dehydrase and lipid transport superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000916Bet v I/Major latex proteinSMARTSM01037Bet_v_1_2coord: 2..154
e-value: 2.5E-17
score: 73.6
IPR000916Bet v I/Major latex proteinPFAMPF00407Bet_v_1coord: 6..153
e-value: 8.9E-29
score: 100.3
IPR023393START-like domain superfamilyGENE3D3.30.530.20coord: 1..152
e-value: 6.4E-34
score: 119.1
NoneNo IPR availablePANTHERPTHR31907:SF52MAJOR LATEX PROTEINcoord: 1..149
NoneNo IPR availablePANTHERPTHR31907MLP-LIKE PROTEIN 423coord: 1..149
NoneNo IPR availableSUPERFAMILY55961Bet v1-likecoord: 1..153

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS019142.1MS019142.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response