MS019136 (gene) Bitter gourd (TR) v1

Overview
NameMS019136
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionNOD26-like intrinsic protein 4;1
Locationscaffold20: 1809708 .. 1812683 (-)
RNA-Seq ExpressionMS019136
SyntenyMS019136
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTCGAAGATCGATGGAGTCGAAGAGGATAAAATCTCACGGCTGGAAGAAGGTACGATCGCCGTCCGCGTCGCCCGTCTCTGTCCCGCATCTATCGTCGCATGCATCCAAAAGGTACCTCTAATCAAATCAAATTAAATCAATTAATCAATCAATCAATCAATCCGCTGTCTATTTCTTAATCAATCAATCAATCGTTTCTCTGTAAAATGTGGTTCGTCAATCAATCAACATTGTATGATCATAAAATGCGTCCGTCTCTGTAAAATTCGGCACACGAATCAATCGAGTATGCAAAGTAAAATGTGTTTGTTTCTCTGTAAAATTCGGCACATGAATCGATCGACTGTATAATGAAACTTGTTCGTTTCGATGGAAGTCGCGGCACAGGAATCAATCAATTGAGTGTATAATTAATTAAAATTGCGGCAGATGAATCGCTAATAAATTGACTGATCAGTGATAAAACGTGTGTCTGTTCTTTTATCTTGGAAATTTTTGTGGCAGTTTATGGCGGAGGTGATCGGAACTTACTTCGTGATCTTCGCCGGATGCGGATCGGTGGCGGTGAACAAGATCTACGGAGGATCGGTGACTTTTCCGGGAATATGTGTGGTTTGGGGATTGATTGTTATGGTTATGATTTACACAGTGGGACACATCTCTGGAGCGCACTTCAACCCCTCAGTTACCATCACACTCGCCATTTTTCGTGGCTTCCCCTGGTGGCAGGTCTCCCTTCTTCACTCTCTTTTTCTTTCTTCTTTCAAATATTCCATATCGCAAATTGCAAATCAGTGAGAATTATAACTTCTAGGCGGTTTGGTTAGTACTCATCGTGATGTGAAAATAGAAATTGAGAAATAGAAATCTGAAAATGGGATTCAATCAAAATAGAGTAATTCCATGTTTTGTCTGTAGCAAATATNTCGACTATGTGTTTGGTATCAAATTTTAAAATTGAATCTCAATTTAAAAAAAAAAAATCTGTGTATTTTTTAATATATTTATAAATTAAATCCATGTATATTTTTTAATATATTTATAAGTGATTGGTTATCTGTTAAATATATAGTTTAATCTAAAATATTATTAGTTAATTTATTAACATCAATATTATAAATATTATAATTTAGTGCAATTATTTATATTTGNATTTGAAAACTAGTGTAGTTACGAACCAAATATTTAGTTGAATAAAAAATAATATTTAATTAATTTATTAATATATATTATATGATTTTTTTAGAATTAGTATTTTACCATATTATATAATTTATAATATATTATATTTTACAAATTTTAATTTTAATACAAATGAAATAAGTTTATAACATCAAATATCAAAATTTTTATATTTTTAAATATAATTATGCATTTTTAAGTTTTATATTCAAATTTTGAATAATATTGAAAACATAAAAAAATTGTTTTCAGAATTTCTTTTGGATTACATAATTTGGGATAGGTTAACAATCATGAACCCCTAAATTCCAACCTACTATCGATTAAATTTAATTTTTCATTTTTTAATTTAGTTTTTATGTTTTTAGATATTAGGTTTGAAACCCTCATGTAAACAATAATGGCAAACATTATATGCAAGAAAAATTGAATAGAAATAATGTGGAAATAAATTAAAAAACGGATGTGAAAAAAAAAACAGCGTTGTCGTTTTTTCGACAAACTGACCGACTGATATTCGGCCCTAGATTTCGGAAACAATATAAATTAATAGTTCAGTTTTATTTGATTTGATTATACCATTTCATGATAATAATGAGATTGATGGTACATACTGAATAATGTTTTTAGGTTCCAATTTACATAGTAGCTCAAGTATCAGGGTCACTTCTTGCAAGCTGCACATTGGATCTAGTGTTTGATGTGACCGTTGAAGCATTCTACGGGACTGTGCCTGCTGGATCTAATGTTCAGTCGTTTGTTCTTGAAATCATCATCACCTTCCTCTTGCTGTTTGTTGTTTTTGCCACCTCCAATAACACCGGTGTAAGTCATCCAAAAACTTGCAACTTTTTATTTTTTCTATAACCTAATGATTAAGATGGTTTATTAGGGACCAAACTATTGCAATAGTATAATATTGTCTACTTTAACCGAAATTTTCGTAACTTATTTTTTATTTCATCTAAAATATCTTAACTCATGTATATATTATGTCTATATAAGATTTTTGTTGCATTCTTATTTTTCTATATTTTAAATAATAGTGTTGAACTATTAGTTCTATTATAAAAATATATATAAGCTTTTGAAGGGTGTCGTCTTAATTTTGTATGGAAAAATTTAGTGGATTTTGAAAACATGTTCTAAGTGTAAGATGTTTAAAAAATAAATAATAATTCTAATGTTTTTTTCTTTCTAAAATAAAAGAATAGCATCATTATTGAGTTCTACAGATTACAAAAGTTTTTTTTTCTTTCATTTGCAGGTTGGGTAAAGAAGTTATTTGAACTTTTAACCTATAAAATGAATTGAACTATATTCAAGTTAACAAATCATTCAATAGTATCTATAAGTAGTATATATTTTTAAGGCGCTTTTCATACTTTACAGGTAGGAGAATTGGGTGGAGTTATTGTTGGCATGACCATTCTCTTAAACGTTTTTGTTGCCGGGTAACTCTACATTCTATCCTTATCATTTAACCATTGTAAATGACTTTTATAACTTGTAAAAGGAAATTTAAAATAATGTTTCAAAAGTCCCCTCAAACACACTTTTACTTATTTTTTGTTAACGATCCTCGAAACACAGGCCGATTTCTGGAGCGTCAATGAATCCAGCGAGGACCCTTGGACCCGCGATAGTGAGGCATGAATTCAGAGGACTATGGGCTTACATTGCGGGGCCGTTGATCGGAGCACTCGCCGGAGGATTCGTATTTGACCTAGTGAAGCCAATAGGGAGCTGACCAGAAGCAGCTCCTGCCTCACCGGCAGCCGGAAAACG

mRNA sequence

ATGGCGTCGAAGATCGATGGAGTCGAAGAGGATAAAATCTCACGGCTGGAAGAAGGTACGATCGCCGTCCGCGTCGCCCGTCTCTGTCCCGCATCTATCGTCGCATGCATCCAAAAGTTTATGGCGGAGGTGATCGGAACTTACTTCGTGATCTTCGCCGGATGCGGATCGGTGGCGGTGAACAAGATCTACGGAGGATCGGTGACTTTTCCGGGAATATGTGTGGTTTGGGGATTGATTGTTATGGTTATGATTTACACAGTGGGACACATCTCTGGAGCGCACTTCAACCCCTCAGTTACCATCACACTCGCCATTTTTCGTGGCTTCCCCTGGTGGCAGGTTCCAATTTACATAGTAGCTCAAGTATCAGGGTCACTTCTTGCAAGCTGCACATTGGATCTAGTGTTTGATGTGACCGTTGAAGCATTCTACGGGACTGTGCCTGCTGGATCTAATGTTCAGTCGTTTGTTCTTGAAATCATCATCACCTTCCTCTTGCTGTTTGTTGTTTTTGCCACCTCCAATAACACCGGTGTAGGAGAATTGGGTGGAGTTATTGTTGGCATGACCATTCTCTTAAACGTTTTTGTTGCCGGGCCGATTTCTGGAGCGTCAATGAATCCAGCGAGGACCCTTGGACCCGCGATAGTGAGGCATGAATTCAGAGGACTATGGGCTTACATTGCGGGGCCGTTGATCGGAGCACTCGCCGGAGGATTCGTATTTGACCTAGTGAAGCCAAGGGAGCTGACCAGAAGCAGCTCCTGCCTCACCGGCAGCCGGAAAACG

Coding sequence (CDS)

ATGGCGTCGAAGATCGATGGAGTCGAAGAGGATAAAATCTCACGGCTGGAAGAAGGTACGATCGCCGTCCGCGTCGCCCGTCTCTGTCCCGCATCTATCGTCGCATGCATCCAAAAGTTTATGGCGGAGGTGATCGGAACTTACTTCGTGATCTTCGCCGGATGCGGATCGGTGGCGGTGAACAAGATCTACGGAGGATCGGTGACTTTTCCGGGAATATGTGTGGTTTGGGGATTGATTGTTATGGTTATGATTTACACAGTGGGACACATCTCTGGAGCGCACTTCAACCCCTCAGTTACCATCACACTCGCCATTTTTCGTGGCTTCCCCTGGTGGCAGGTTCCAATTTACATAGTAGCTCAAGTATCAGGGTCACTTCTTGCAAGCTGCACATTGGATCTAGTGTTTGATGTGACCGTTGAAGCATTCTACGGGACTGTGCCTGCTGGATCTAATGTTCAGTCGTTTGTTCTTGAAATCATCATCACCTTCCTCTTGCTGTTTGTTGTTTTTGCCACCTCCAATAACACCGGTGTAGGAGAATTGGGTGGAGTTATTGTTGGCATGACCATTCTCTTAAACGTTTTTGTTGCCGGGCCGATTTCTGGAGCGTCAATGAATCCAGCGAGGACCCTTGGACCCGCGATAGTGAGGCATGAATTCAGAGGACTATGGGCTTACATTGCGGGGCCGTTGATCGGAGCACTCGCCGGAGGATTCGTATTTGACCTAGTGAAGCCAAGGGAGCTGACCAGAAGCAGCTCCTGCCTCACCGGCAGCCGGAAAACG

Protein sequence

MASKIDGVEEDKISRLEEGTIAVRVARLCPASIVACIQKFMAEVIGTYFVIFAGCGSVAVNKIYGGSVTFPGICVVWGLIVMVMIYTVGHISGAHFNPSVTITLAIFRGFPWWQVPIYIVAQVSGSLLASCTLDLVFDVTVEAFYGTVPAGSNVQSFVLEIIITFLLLFVVFATSNNTGVGELGGVIVGMTILLNVFVAGPISGASMNPARTLGPAIVRHEFRGLWAYIAGPLIGALAGGFVFDLVKPRELTRSSSCLTGSRKT
Homology
BLAST of MS019136 vs. NCBI nr
Match: XP_022143298.1 (probable aquaporin NIP-type [Momordica charantia])

HSP 1 Score: 484.2 bits (1245), Expect = 7.2e-133
Identity = 248/248 (100.00%), Postives = 248/248 (100.00%), Query Frame = 0

Query: 1   MASKIDGVEEDKISRLEEGTIAVRVARLCPASIVACIQKFMAEVIGTYFVIFAGCGSVAV 60
           MASKIDGVEEDKISRLEEGTIAVRVARLCPASIVACIQKFMAEVIGTYFVIFAGCGSVAV
Sbjct: 1   MASKIDGVEEDKISRLEEGTIAVRVARLCPASIVACIQKFMAEVIGTYFVIFAGCGSVAV 60

Query: 61  NKIYGGSVTFPGICVVWGLIVMVMIYTVGHISGAHFNPSVTITLAIFRGFPWWQVPIYIV 120
           NKIYGGSVTFPGICVVWGLIVMVMIYTVGHISGAHFNPSVTITLAIFRGFPWWQVPIYIV
Sbjct: 61  NKIYGGSVTFPGICVVWGLIVMVMIYTVGHISGAHFNPSVTITLAIFRGFPWWQVPIYIV 120

Query: 121 AQVSGSLLASCTLDLVFDVTVEAFYGTVPAGSNVQSFVLEIIITFLLLFVVFATSNNTGV 180
           AQVSGSLLASCTLDLVFDVTVEAFYGTVPAGSNVQSFVLEIIITFLLLFVVFATSNNTGV
Sbjct: 121 AQVSGSLLASCTLDLVFDVTVEAFYGTVPAGSNVQSFVLEIIITFLLLFVVFATSNNTGV 180

Query: 181 GELGGVIVGMTILLNVFVAGPISGASMNPARTLGPAIVRHEFRGLWAYIAGPLIGALAGG 240
           GELGGVIVGMTILLNVFVAGPISGASMNPARTLGPAIVRHEFRGLWAYIAGPLIGALAGG
Sbjct: 181 GELGGVIVGMTILLNVFVAGPISGASMNPARTLGPAIVRHEFRGLWAYIAGPLIGALAGG 240

Query: 241 FVFDLVKP 249
           FVFDLVKP
Sbjct: 241 FVFDLVKP 248

BLAST of MS019136 vs. NCBI nr
Match: NP_001380720.1 (aquaporin NIP4-1 [Cucumis melo] >KAA0060766.1 putative aquaporin NIP-type [Cucumis melo var. makuwa])

HSP 1 Score: 382.9 bits (982), Expect = 2.3e-102
Identity = 194/270 (71.85%), Postives = 235/270 (87.04%), Query Frame = 0

Query: 1   MASKIDGVEEDKISRLEEGTIAVRVARLCPASIVACIQKFMAEVIGTYFVIFAGCGSVAV 60
           MA+KIDG+E+++IS+LEEGT+   +ARLCP++ V  IQK +AE+IGTYFVIF+GCG+VAV
Sbjct: 1   MATKIDGIEDEEISKLEEGTVVSAIARLCPSNSVVIIQKVIAELIGTYFVIFSGCGAVAV 60

Query: 61  NKIYGGSVTFPGICVVWGLIVMVMIYTVGHISGAHFNPSVTITLAIFRGFPWWQVPIYIV 120
           NKIY GSVTFPGICVVWGLIVMVM+Y+VGH+SGAHFNP+VT T A+FR FP+WQVPIY  
Sbjct: 61  NKIY-GSVTFPGICVVWGLIVMVMVYSVGHVSGAHFNPAVTFTFALFRRFPFWQVPIYTG 120

Query: 121 AQVSGSLLASCTLDLVFDVTVEAFYGTVPAGSNVQSFVLEIIITFLLLFVVFATS-NNTG 180
           AQ+ GSLLASCTLDL+ +VT EAF+GTVP GSNVQS VLEIIITFLL+FV+   S +N  
Sbjct: 121 AQLMGSLLASCTLDLMLEVTPEAFFGTVPVGSNVQSLVLEIIITFLLMFVISGVSTDNRA 180

Query: 181 VGELGGVIVGMTILLNVFVAGPISGASMNPARTLGPAIVRHEFRGLWAYIAGPLIGALAG 240
           VGELGG++VGMTILLNVFVAGPISGASMNPART+GPAIV+ +F+GLW YI GP IGA+AG
Sbjct: 181 VGELGGIVVGMTILLNVFVAGPISGASMNPARTIGPAIVKRQFKGLWVYIVGPFIGAVAG 240

Query: 241 GFVFDLV----KP-RELTRSSSCLTGSRKT 265
           GFV++L+    KP RE+TRS+S LTG+ K+
Sbjct: 241 GFVYNLMRYTDKPLREITRSTSFLTGTLKS 269

BLAST of MS019136 vs. NCBI nr
Match: XP_004149794.1 (probable aquaporin NIP-type [Cucumis sativus] >KGN57839.1 hypothetical protein Csa_010655 [Cucumis sativus])

HSP 1 Score: 380.2 bits (975), Expect = 1.5e-101
Identity = 191/270 (70.74%), Postives = 234/270 (86.67%), Query Frame = 0

Query: 1   MASKIDGVEEDKISRLEEGTIAVRVARLCPASIVACIQKFMAEVIGTYFVIFAGCGSVAV 60
           MA+KIDG+E+++IS+LEEG +   VAR CP+++V  IQK +AE+IGTYFVIF GCG+V V
Sbjct: 1   MATKIDGIEDEEISKLEEGIVVSAVARFCPSNLVVIIQKVIAELIGTYFVIFGGCGAVVV 60

Query: 61  NKIYGGSVTFPGICVVWGLIVMVMIYTVGHISGAHFNPSVTITLAIFRGFPWWQVPIYIV 120
           NKIY GSVTFPGICVVWGLIVMVM+Y+VGH+SGAHFNP+VT+T A+FR FP+WQVPIY  
Sbjct: 61  NKIY-GSVTFPGICVVWGLIVMVMVYSVGHVSGAHFNPAVTLTFALFRRFPFWQVPIYTG 120

Query: 121 AQVSGSLLASCTLDLVFDVTVEAFYGTVPAGSNVQSFVLEIIITFLLLFVVFATS-NNTG 180
           AQ+ GSLLASCTLDL+F+VT EAF+GTVP GSNVQS V+EIIITFLL+FV+   S +N  
Sbjct: 121 AQLMGSLLASCTLDLMFEVTPEAFFGTVPVGSNVQSLVIEIIITFLLMFVISGVSTDNRA 180

Query: 181 VGELGGVIVGMTILLNVFVAGPISGASMNPARTLGPAIVRHEFRGLWAYIAGPLIGALAG 240
           VGELGGV+VGMTILLNVFVAGPISGASMNPAR+LGPAIV+ +F+GLW Y+ GPLIGA+AG
Sbjct: 181 VGELGGVMVGMTILLNVFVAGPISGASMNPARSLGPAIVKRQFKGLWVYMLGPLIGAVAG 240

Query: 241 GFVFDLVK-----PRELTRSSSCLTGSRKT 265
           GFV++L++      RE+TRS+S LTG+ K+
Sbjct: 241 GFVYNLMRYTDKSLREITRSTSFLTGTHKS 269

BLAST of MS019136 vs. NCBI nr
Match: XP_038882565.1 (probable aquaporin NIP-type [Benincasa hispida])

HSP 1 Score: 379.0 bits (972), Expect = 3.3e-101
Identity = 193/270 (71.48%), Postives = 233/270 (86.30%), Query Frame = 0

Query: 1   MASKIDGVEEDKISRLEEGTIAVRVARLCPASIVACIQKFMAEVIGTYFVIFAGCGSVAV 60
           MA+KIDG+EE++IS+LEEGT+   VARLCP++ V  IQK +AEVIGTYFVIFAGCG+VAV
Sbjct: 1   MATKIDGIEEEEISKLEEGTLVSAVARLCPSTSVVIIQKVIAEVIGTYFVIFAGCGAVAV 60

Query: 61  NKIYGGSVTFPGICVVWGLIVMVMIYTVGHISGAHFNPSVTITLAIFRGFPWWQVPIYIV 120
           NKIY GSVTFPGICVVWGLIVMVM+Y+VGHISGAHFNP+VT+T A FR FP+WQVPIY+ 
Sbjct: 61  NKIY-GSVTFPGICVVWGLIVMVMVYSVGHISGAHFNPAVTLTFAFFRRFPFWQVPIYVG 120

Query: 121 AQVSGSLLASCTLDLVFDVTVEAFYGTVPAGSNVQSFVLEIIITFLLLFVVFATS-NNTG 180
           AQ+ GSLLASCTL+L+ +VT EAF+GT P GSN+QS VLEIIITFLL+FV+   S +N  
Sbjct: 121 AQLMGSLLASCTLELMLEVTPEAFFGTEPVGSNLQSLVLEIIITFLLMFVISGVSTDNRA 180

Query: 181 VGELGGVIVGMTILLNVFVAGPISGASMNPARTLGPAIVRHEFRGLWAYIAGPLIGALAG 240
           VGE  G++VGMTILLNVFVAGPISGASMNPAR++GPAIV+ +F+GLW Y+ GPLIGA+AG
Sbjct: 181 VGEFAGIVVGMTILLNVFVAGPISGASMNPARSIGPAIVKRQFKGLWVYVVGPLIGAVAG 240

Query: 241 GFVFDLV----KP-RELTRSSSCLTGSRKT 265
           GFV++L+    KP  E+TRSSS  TGS+K+
Sbjct: 241 GFVYNLIRFTDKPLGEITRSSSFFTGSQKS 269

BLAST of MS019136 vs. NCBI nr
Match: XP_022948505.1 (putative aquaporin NIP4-1 [Cucurbita moschata] >KAG6607007.1 putative aquaporin NIP4-1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036706.1 putative aquaporin NIP4-1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 364.8 bits (935), Expect = 6.4e-97
Identity = 193/270 (71.48%), Postives = 231/270 (85.56%), Query Frame = 0

Query: 1   MASKIDGVEEDKISRLEEGTIAVRVARLCPASIVACIQKFMAEVIGTYFVIFAGCGSVAV 60
           MA+K DG++E++IS+LEEGT+   VARL P+S VA IQK +AEVIGTYFVIF GCG+V V
Sbjct: 1   MATKTDGIQEEEISKLEEGTLGTCVARLRPSSWVAIIQKVIAEVIGTYFVIFTGCGAVVV 60

Query: 61  NKIYGGSVTFPGICVVWGLIVMVMIYTVGHISGAHFNPSVTITLAIFRGFPWWQVPIYIV 120
           NKIY GSVTFPGICVVWGLIVMVM+Y++GH+SGAHFNPSVTIT AIFR FP+ QVPIYI 
Sbjct: 61  NKIY-GSVTFPGICVVWGLIVMVMVYSLGHVSGAHFNPSVTITFAIFRRFPFCQVPIYIG 120

Query: 121 AQVSGSLLASCTLDLVFDVTVEAFYGTVPAGSNVQSFVLEIIITFLLLFVVFATS-NNTG 180
           AQV+GSLLAS TLDL+F+VT EAF+GTVPAGS+VQSFVLEIIITFLLL VV   S ++  
Sbjct: 121 AQVAGSLLASTTLDLLFEVTPEAFFGTVPAGSDVQSFVLEIIITFLLLLVVSGVSTDHRA 180

Query: 181 VGELGGVIVGMTILLNVFVAGPISGASMNPARTLGPAIVRHEFRGLWAYIAGPLIGALAG 240
           VGEL G++VGMT+LLNVFVAGPISGASMNPART+GPAIV+ +F+GLW Y+ GPLIGA+AG
Sbjct: 181 VGELAGIVVGMTVLLNVFVAGPISGASMNPARTIGPAIVKGQFKGLWVYMVGPLIGAIAG 240

Query: 241 GFVFDLV----KP-RELTRSSSCLTGSRKT 265
            F ++L+    KP  E+T+SSS L  S+K+
Sbjct: 241 AFTYNLMRFTDKPLSEITKSSSFLNRSQKS 269

BLAST of MS019136 vs. ExPASy Swiss-Prot
Match: P49173 (Probable aquaporin NIP-type OS=Nicotiana alata OX=4087 PE=2 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 1.5e-85
Identity = 167/268 (62.31%), Postives = 210/268 (78.36%), Query Frame = 0

Query: 3   SKIDGVEEDKISRLEEGTI------AVRVARLCPASIVACIQKFMAEVIGTYFVIFAGCG 62
           +K DG +E++IS++EEG I         V      S+V  +QK +AE IGTYFVIFAGCG
Sbjct: 2   AKKDGNQEEEISQMEEGNIHSASNSDSNVGFCSSVSVVVILQKLIAEAIGTYFVIFAGCG 61

Query: 63  SVAVNKIYGGSVTFPGICVVWGLIVMVMIYTVGHISGAHFNPSVTITLAIFRGFPWWQVP 122
           SVAVNKIY GSVTFPGICV WGLIVMVM+YTVG+ISGAHFNP+VTIT +IF  FPW QVP
Sbjct: 62  SVAVNKIY-GSVTFPGICVTWGLIVMVMVYTVGYISGAHFNPAVTITFSIFGRFPWKQVP 121

Query: 123 IYIVAQVSGSLLASCTLDLVFDVTVEAFYGTVPAGSNVQSFVLEIIITFLLLFVVF-ATS 182
           +YI+AQ+ GS+LAS TL L+FDVT +A++GTVP GSN QS  +EIII+FLL+FV+    +
Sbjct: 122 LYIIAQLMGSILASGTLALLFDVTPQAYFGTVPVGSNGQSLAIEIIISFLLMFVISGVAT 181

Query: 183 NNTGVGELGGVIVGMTILLNVFVAGPISGASMNPARTLGPAIVRHEFRGLWAYIAGPLIG 242
           ++  +G++ G+ VGMTI LNVFVAGPISGASMNPAR++GPAIV+H + GLW Y+ GP+IG
Sbjct: 182 DDRAIGQVAGIAVGMTITLNVFVAGPISGASMNPARSIGPAIVKHVYTGLWVYVVGPIIG 241

Query: 243 ALAGGFVFDLV----KP-RELTRSSSCL 259
            LAG FV++L+    KP REL +S+S L
Sbjct: 242 TLAGAFVYNLIRSTDKPLRELAKSASSL 268

BLAST of MS019136 vs. ExPASy Swiss-Prot
Match: Q8W036 (Probable aquaporin NIP4-2 OS=Arabidopsis thaliana OX=3702 GN=NIP4-2 PE=2 SV=2)

HSP 1 Score: 306.6 bits (784), Expect = 2.7e-82
Identity = 158/269 (58.74%), Postives = 205/269 (76.21%), Query Frame = 0

Query: 1   MASKIDGVEEDKISRLEEGTI-----AVRVARLCPASIVACIQKFMAEVIGTYFVIFAGC 60
           M S  + +E+++ISR+E+G        +  A     SIV   QK +AE+IGTYF+IF+GC
Sbjct: 1   MTSHGEEIEDEQISRIEKGNCKDSQGGMETAICSSPSIVCLTQKLIAEMIGTYFIIFSGC 60

Query: 61  GSVAVNKIYGGSVTFPGICVVWGLIVMVMIYTVGHISGAHFNPSVTITLAIFRGFPWWQV 120
           G V VN +YGG++TFPGICV WGLIVMVMIY+ GHISGAHFNP+VT+T A+FR FPW+QV
Sbjct: 61  GVVVVNVLYGGTITFPGICVTWGLIVMVMIYSTGHISGAHFNPAVTVTFAVFRRFPWYQV 120

Query: 121 PIYIVAQVSGSLLASCTLDLVFDVTVEAFYGTVPAGSNVQSFVLEIIITFLLLFVVFATS 180
           P+YI AQ++GSLLAS TL L+F+VT +AF+GT P  S+ Q+ V EIII+FLL+FV+   +
Sbjct: 121 PLYIGAQLTGSLLASLTLRLMFNVTPKAFFGTTPTDSSGQALVAEIIISFLLMFVISGVA 180

Query: 181 NNT-GVGELGGVIVGMTILLNVFVAGPISGASMNPARTLGPAIVRHEFRGLWAYIAGPLI 240
            ++   GEL G+ VGMTI+LNVFVAGPISGASMNPAR+LGPAIV   ++G+W YI GP +
Sbjct: 181 TDSRATGELAGIAVGMTIILNVFVAGPISGASMNPARSLGPAIVMGRYKGIWVYIVGPFV 240

Query: 241 GALAGGFVFDLV----KP-RELTRSSSCL 259
           G  AGGFV++ +    KP RELT+S+S L
Sbjct: 241 GIFAGGFVYNFMRFTDKPLRELTKSASFL 269

BLAST of MS019136 vs. ExPASy Swiss-Prot
Match: Q9FIZ9 (Putative aquaporin NIP4-1 OS=Arabidopsis thaliana OX=3702 GN=NIP4-1 PE=3 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 3.5e-82
Identity = 159/269 (59.11%), Postives = 205/269 (76.21%), Query Frame = 0

Query: 1   MASKIDGVEEDKISRLEEG-----TIAVRVARLCPASIVACIQKFMAEVIGTYFVIFAGC 60
           M+S  D +EE++ISR+E+G        +        SIV   QK +AE+IGTYF++F+GC
Sbjct: 1   MSSHSDEIEEEQISRIEKGKGKDCQGGIETVICTSPSIVCLTQKLIAEMIGTYFIVFSGC 60

Query: 61  GSVAVNKIYGGSVTFPGICVVWGLIVMVMIYTVGHISGAHFNPSVTITLAIFRGFPWWQV 120
           G V VN +YGG++TFPGICV WGLIVMVMIY+ GHISGAHFNP+VT+T AIFR FPW QV
Sbjct: 61  GVVVVNVLYGGTITFPGICVTWGLIVMVMIYSTGHISGAHFNPAVTVTFAIFRRFPWHQV 120

Query: 121 PIYIVAQVSGSLLASCTLDLVFDVTVEAFYGTVPAGSNVQSFVLEIIITFLLLFVVF-AT 180
           P+YI AQ +GSLLAS TL L+F VT EAF+GT PA S  ++ V EIII+FLL+FV+    
Sbjct: 121 PLYIGAQFAGSLLASLTLRLMFKVTPEAFFGTTPADSPARALVAEIIISFLLMFVISGVA 180

Query: 181 SNNTGVGELGGVIVGMTILLNVFVAGPISGASMNPARTLGPAIVRHEFRGLWAYIAGPLI 240
           ++N  VGEL G+ VGMTI++NVFVAGPISGASMNPAR+LGPA+V   ++ +W YI GP++
Sbjct: 181 TDNRAVGELAGIAVGMTIMVNVFVAGPISGASMNPARSLGPALVMGVYKHIWVYIVGPVL 240

Query: 241 GALAGGFVFDLV----KP-RELTRSSSCL 259
           G ++GGFV++L+    KP RELT+S+S L
Sbjct: 241 GVISGGFVYNLIRFTDKPLRELTKSASFL 269

BLAST of MS019136 vs. ExPASy Swiss-Prot
Match: Q40746 (Aquaporin NIP1-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP1-1 PE=2 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 2.2e-68
Identity = 133/231 (57.58%), Postives = 172/231 (74.46%), Query Frame = 0

Query: 34  VACIQKFMAEVIGTYFVIFAGCGSVAVNKIYGGSVTFPGICVVWGLIVMVMIYTVGHISG 93
           V  IQK +AE+ GTYF+IFAGCG+V +N+   G +TFPG+ +VWGL VMVM+Y VGHISG
Sbjct: 44  VPFIQKIIAEIFGTYFLIFAGCGAVTINQSKNGQITFPGVAIVWGLAVMVMVYAVGHISG 103

Query: 94  AHFNPSVTITLAIFRGFPWWQVPIYIVAQVSGSLLASCTLDLVFDVTVEAFYGTVPAGSN 153
           AHFNP+VT+  A  R FPW QVP Y  AQ+ G+ LA+ TL L+F    E F GT+PAGS+
Sbjct: 104 AHFNPAVTLAFATCRRFPWRQVPAYAAAQMLGATLAAGTLRLMFGGRHEHFPGTLPAGSD 163

Query: 154 VQSFVLEIIITFLLLFVVF-ATSNNTGVGELGGVIVGMTILLNVFVAGPISGASMNPART 213
           VQS VLE IITF L+FV+    ++N  +GEL G+ VG TILLNV +AGPISGASMNPAR+
Sbjct: 164 VQSLVLEFIITFYLMFVISGVATDNRAIGELAGLAVGATILLNVLIAGPISGASMNPARS 223

Query: 214 LGPAIVRHEFRGLWAYIAGPLIGALAGGFVFDLV----KP-RELTRSSSCL 259
           LGPA++  E+R +W YI GP+ GA+AG + ++++    KP RE+T+S S L
Sbjct: 224 LGPAMIGGEYRSIWVYIVGPVAGAVAGAWAYNIIRFTNKPLREITKSGSFL 274

BLAST of MS019136 vs. ExPASy Swiss-Prot
Match: Q0DK16 (Aquaporin NIP1-3 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP1-3 PE=3 SV=2)

HSP 1 Score: 258.1 bits (658), Expect = 1.1e-67
Identity = 132/240 (55.00%), Postives = 173/240 (72.08%), Query Frame = 0

Query: 30  PASIVACIQKFMAEVIGTYFVIFAGCGSVAVNKIYGGSVTFPGICVVWGLIVMVMIYTVG 89
           P   V   QK +AE++GT+F+IFAGC +VAVNK  GG+VTFPGIC+ WGL VMVM+Y+VG
Sbjct: 46  PMFSVQFAQKVIAEILGTFFLIFAGCAAVAVNKRTGGTVTFPGICITWGLAVMVMVYSVG 105

Query: 90  HISGAHFNPSVTITLAIFRGFPWWQVPIYIVAQVSGSLLASCTLDLVFDVTVEAFYGTVP 149
           HISGAH NP+VT+  A    FPW +VP Y  AQV+GS  AS  L  +F    E F+GT P
Sbjct: 106 HISGAHLNPAVTLAFATCGRFPWRRVPAYAAAQVAGSAAASAALRALFGGAPEHFFGTAP 165

Query: 150 AGSNVQSFVLEIIITFLLLFVVF-ATSNNTGVGELGGVIVGMTILLNVFVAGPISGASMN 209
           AGS+VQS  +E IITF L+FVV    ++N  +GEL G+ VG T+L+NV  AGPISGASMN
Sbjct: 166 AGSDVQSLAMEFIITFYLMFVVSGVATDNRAIGELAGLAVGATVLVNVLFAGPISGASMN 225

Query: 210 PARTLGPAIVRHEFRGLWAYIAGPLIGALAGGFVFDLV----KP-RELTRSSSCLTGSRK 264
           PART+GPAI+   + G+W YIAGP+ GA+AG + ++L+    KP RE+T ++S +  +R+
Sbjct: 226 PARTIGPAIILGRYTGIWVYIAGPVFGAVAGAWAYNLIRFTDKPLREITMTASFIRSTRR 285

BLAST of MS019136 vs. ExPASy TrEMBL
Match: A0A6J1CQD3 (probable aquaporin NIP-type OS=Momordica charantia OX=3673 GN=LOC111013203 PE=3 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 3.5e-133
Identity = 248/248 (100.00%), Postives = 248/248 (100.00%), Query Frame = 0

Query: 1   MASKIDGVEEDKISRLEEGTIAVRVARLCPASIVACIQKFMAEVIGTYFVIFAGCGSVAV 60
           MASKIDGVEEDKISRLEEGTIAVRVARLCPASIVACIQKFMAEVIGTYFVIFAGCGSVAV
Sbjct: 1   MASKIDGVEEDKISRLEEGTIAVRVARLCPASIVACIQKFMAEVIGTYFVIFAGCGSVAV 60

Query: 61  NKIYGGSVTFPGICVVWGLIVMVMIYTVGHISGAHFNPSVTITLAIFRGFPWWQVPIYIV 120
           NKIYGGSVTFPGICVVWGLIVMVMIYTVGHISGAHFNPSVTITLAIFRGFPWWQVPIYIV
Sbjct: 61  NKIYGGSVTFPGICVVWGLIVMVMIYTVGHISGAHFNPSVTITLAIFRGFPWWQVPIYIV 120

Query: 121 AQVSGSLLASCTLDLVFDVTVEAFYGTVPAGSNVQSFVLEIIITFLLLFVVFATSNNTGV 180
           AQVSGSLLASCTLDLVFDVTVEAFYGTVPAGSNVQSFVLEIIITFLLLFVVFATSNNTGV
Sbjct: 121 AQVSGSLLASCTLDLVFDVTVEAFYGTVPAGSNVQSFVLEIIITFLLLFVVFATSNNTGV 180

Query: 181 GELGGVIVGMTILLNVFVAGPISGASMNPARTLGPAIVRHEFRGLWAYIAGPLIGALAGG 240
           GELGGVIVGMTILLNVFVAGPISGASMNPARTLGPAIVRHEFRGLWAYIAGPLIGALAGG
Sbjct: 181 GELGGVIVGMTILLNVFVAGPISGASMNPARTLGPAIVRHEFRGLWAYIAGPLIGALAGG 240

Query: 241 FVFDLVKP 249
           FVFDLVKP
Sbjct: 241 FVFDLVKP 248

BLAST of MS019136 vs. ExPASy TrEMBL
Match: A0A5A7V2N2 (Putative aquaporin NIP-type OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold72G00660 PE=3 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 1.1e-102
Identity = 194/270 (71.85%), Postives = 235/270 (87.04%), Query Frame = 0

Query: 1   MASKIDGVEEDKISRLEEGTIAVRVARLCPASIVACIQKFMAEVIGTYFVIFAGCGSVAV 60
           MA+KIDG+E+++IS+LEEGT+   +ARLCP++ V  IQK +AE+IGTYFVIF+GCG+VAV
Sbjct: 1   MATKIDGIEDEEISKLEEGTVVSAIARLCPSNSVVIIQKVIAELIGTYFVIFSGCGAVAV 60

Query: 61  NKIYGGSVTFPGICVVWGLIVMVMIYTVGHISGAHFNPSVTITLAIFRGFPWWQVPIYIV 120
           NKIY GSVTFPGICVVWGLIVMVM+Y+VGH+SGAHFNP+VT T A+FR FP+WQVPIY  
Sbjct: 61  NKIY-GSVTFPGICVVWGLIVMVMVYSVGHVSGAHFNPAVTFTFALFRRFPFWQVPIYTG 120

Query: 121 AQVSGSLLASCTLDLVFDVTVEAFYGTVPAGSNVQSFVLEIIITFLLLFVVFATS-NNTG 180
           AQ+ GSLLASCTLDL+ +VT EAF+GTVP GSNVQS VLEIIITFLL+FV+   S +N  
Sbjct: 121 AQLMGSLLASCTLDLMLEVTPEAFFGTVPVGSNVQSLVLEIIITFLLMFVISGVSTDNRA 180

Query: 181 VGELGGVIVGMTILLNVFVAGPISGASMNPARTLGPAIVRHEFRGLWAYIAGPLIGALAG 240
           VGELGG++VGMTILLNVFVAGPISGASMNPART+GPAIV+ +F+GLW YI GP IGA+AG
Sbjct: 181 VGELGGIVVGMTILLNVFVAGPISGASMNPARTIGPAIVKRQFKGLWVYIVGPFIGAVAG 240

Query: 241 GFVFDLV----KP-RELTRSSSCLTGSRKT 265
           GFV++L+    KP RE+TRS+S LTG+ K+
Sbjct: 241 GFVYNLMRYTDKPLREITRSTSFLTGTLKS 269

BLAST of MS019136 vs. ExPASy TrEMBL
Match: A0A1S3C5C4 (probable aquaporin NIP-type OS=Cucumis melo OX=3656 GN=LOC103496839 PE=3 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 1.1e-102
Identity = 194/270 (71.85%), Postives = 235/270 (87.04%), Query Frame = 0

Query: 1   MASKIDGVEEDKISRLEEGTIAVRVARLCPASIVACIQKFMAEVIGTYFVIFAGCGSVAV 60
           MA+KIDG+E+++IS+LEEGT+   +ARLCP++ V  IQK +AE+IGTYFVIF+GCG+VAV
Sbjct: 1   MATKIDGIEDEEISKLEEGTVVSAIARLCPSNSVVIIQKVIAELIGTYFVIFSGCGAVAV 60

Query: 61  NKIYGGSVTFPGICVVWGLIVMVMIYTVGHISGAHFNPSVTITLAIFRGFPWWQVPIYIV 120
           NKIY GSVTFPGICVVWGLIVMVM+Y+VGH+SGAHFNP+VT T A+FR FP+WQVPIY  
Sbjct: 61  NKIY-GSVTFPGICVVWGLIVMVMVYSVGHVSGAHFNPAVTFTFALFRRFPFWQVPIYTG 120

Query: 121 AQVSGSLLASCTLDLVFDVTVEAFYGTVPAGSNVQSFVLEIIITFLLLFVVFATS-NNTG 180
           AQ+ GSLLASCTLDL+ +VT EAF+GTVP GSNVQS VLEIIITFLL+FV+   S +N  
Sbjct: 121 AQLMGSLLASCTLDLMLEVTPEAFFGTVPVGSNVQSLVLEIIITFLLMFVISGVSTDNRA 180

Query: 181 VGELGGVIVGMTILLNVFVAGPISGASMNPARTLGPAIVRHEFRGLWAYIAGPLIGALAG 240
           VGELGG++VGMTILLNVFVAGPISGASMNPART+GPAIV+ +F+GLW YI GP IGA+AG
Sbjct: 181 VGELGGIVVGMTILLNVFVAGPISGASMNPARTIGPAIVKRQFKGLWVYIVGPFIGAVAG 240

Query: 241 GFVFDLV----KP-RELTRSSSCLTGSRKT 265
           GFV++L+    KP RE+TRS+S LTG+ K+
Sbjct: 241 GFVYNLMRYTDKPLREITRSTSFLTGTLKS 269

BLAST of MS019136 vs. ExPASy TrEMBL
Match: A0A0A0LA39 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G345890 PE=3 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 7.1e-102
Identity = 191/270 (70.74%), Postives = 234/270 (86.67%), Query Frame = 0

Query: 1   MASKIDGVEEDKISRLEEGTIAVRVARLCPASIVACIQKFMAEVIGTYFVIFAGCGSVAV 60
           MA+KIDG+E+++IS+LEEG +   VAR CP+++V  IQK +AE+IGTYFVIF GCG+V V
Sbjct: 1   MATKIDGIEDEEISKLEEGIVVSAVARFCPSNLVVIIQKVIAELIGTYFVIFGGCGAVVV 60

Query: 61  NKIYGGSVTFPGICVVWGLIVMVMIYTVGHISGAHFNPSVTITLAIFRGFPWWQVPIYIV 120
           NKIY GSVTFPGICVVWGLIVMVM+Y+VGH+SGAHFNP+VT+T A+FR FP+WQVPIY  
Sbjct: 61  NKIY-GSVTFPGICVVWGLIVMVMVYSVGHVSGAHFNPAVTLTFALFRRFPFWQVPIYTG 120

Query: 121 AQVSGSLLASCTLDLVFDVTVEAFYGTVPAGSNVQSFVLEIIITFLLLFVVFATS-NNTG 180
           AQ+ GSLLASCTLDL+F+VT EAF+GTVP GSNVQS V+EIIITFLL+FV+   S +N  
Sbjct: 121 AQLMGSLLASCTLDLMFEVTPEAFFGTVPVGSNVQSLVIEIIITFLLMFVISGVSTDNRA 180

Query: 181 VGELGGVIVGMTILLNVFVAGPISGASMNPARTLGPAIVRHEFRGLWAYIAGPLIGALAG 240
           VGELGGV+VGMTILLNVFVAGPISGASMNPAR+LGPAIV+ +F+GLW Y+ GPLIGA+AG
Sbjct: 181 VGELGGVMVGMTILLNVFVAGPISGASMNPARSLGPAIVKRQFKGLWVYMLGPLIGAVAG 240

Query: 241 GFVFDLVK-----PRELTRSSSCLTGSRKT 265
           GFV++L++      RE+TRS+S LTG+ K+
Sbjct: 241 GFVYNLMRYTDKSLREITRSTSFLTGTHKS 269

BLAST of MS019136 vs. ExPASy TrEMBL
Match: A0A6J1GA14 (putative aquaporin NIP4-1 OS=Cucurbita moschata OX=3662 GN=LOC111452164 PE=3 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 3.1e-97
Identity = 193/270 (71.48%), Postives = 231/270 (85.56%), Query Frame = 0

Query: 1   MASKIDGVEEDKISRLEEGTIAVRVARLCPASIVACIQKFMAEVIGTYFVIFAGCGSVAV 60
           MA+K DG++E++IS+LEEGT+   VARL P+S VA IQK +AEVIGTYFVIF GCG+V V
Sbjct: 1   MATKTDGIQEEEISKLEEGTLGTCVARLRPSSWVAIIQKVIAEVIGTYFVIFTGCGAVVV 60

Query: 61  NKIYGGSVTFPGICVVWGLIVMVMIYTVGHISGAHFNPSVTITLAIFRGFPWWQVPIYIV 120
           NKIY GSVTFPGICVVWGLIVMVM+Y++GH+SGAHFNPSVTIT AIFR FP+ QVPIYI 
Sbjct: 61  NKIY-GSVTFPGICVVWGLIVMVMVYSLGHVSGAHFNPSVTITFAIFRRFPFCQVPIYIG 120

Query: 121 AQVSGSLLASCTLDLVFDVTVEAFYGTVPAGSNVQSFVLEIIITFLLLFVVFATS-NNTG 180
           AQV+GSLLAS TLDL+F+VT EAF+GTVPAGS+VQSFVLEIIITFLLL VV   S ++  
Sbjct: 121 AQVAGSLLASTTLDLLFEVTPEAFFGTVPAGSDVQSFVLEIIITFLLLLVVSGVSTDHRA 180

Query: 181 VGELGGVIVGMTILLNVFVAGPISGASMNPARTLGPAIVRHEFRGLWAYIAGPLIGALAG 240
           VGEL G++VGMT+LLNVFVAGPISGASMNPART+GPAIV+ +F+GLW Y+ GPLIGA+AG
Sbjct: 181 VGELAGIVVGMTVLLNVFVAGPISGASMNPARTIGPAIVKGQFKGLWVYMVGPLIGAIAG 240

Query: 241 GFVFDLV----KP-RELTRSSSCLTGSRKT 265
            F ++L+    KP  E+T+SSS L  S+K+
Sbjct: 241 AFTYNLMRFTDKPLSEITKSSSFLNRSQKS 269

BLAST of MS019136 vs. TAIR 10
Match: AT5G37820.1 (NOD26-like intrinsic protein 4;2 )

HSP 1 Score: 306.6 bits (784), Expect = 1.9e-83
Identity = 158/269 (58.74%), Postives = 205/269 (76.21%), Query Frame = 0

Query: 1   MASKIDGVEEDKISRLEEGTI-----AVRVARLCPASIVACIQKFMAEVIGTYFVIFAGC 60
           M S  + +E+++ISR+E+G        +  A     SIV   QK +AE+IGTYF+IF+GC
Sbjct: 1   MTSHGEEIEDEQISRIEKGNCKDSQGGMETAICSSPSIVCLTQKLIAEMIGTYFIIFSGC 60

Query: 61  GSVAVNKIYGGSVTFPGICVVWGLIVMVMIYTVGHISGAHFNPSVTITLAIFRGFPWWQV 120
           G V VN +YGG++TFPGICV WGLIVMVMIY+ GHISGAHFNP+VT+T A+FR FPW+QV
Sbjct: 61  GVVVVNVLYGGTITFPGICVTWGLIVMVMIYSTGHISGAHFNPAVTVTFAVFRRFPWYQV 120

Query: 121 PIYIVAQVSGSLLASCTLDLVFDVTVEAFYGTVPAGSNVQSFVLEIIITFLLLFVVFATS 180
           P+YI AQ++GSLLAS TL L+F+VT +AF+GT P  S+ Q+ V EIII+FLL+FV+   +
Sbjct: 121 PLYIGAQLTGSLLASLTLRLMFNVTPKAFFGTTPTDSSGQALVAEIIISFLLMFVISGVA 180

Query: 181 NNT-GVGELGGVIVGMTILLNVFVAGPISGASMNPARTLGPAIVRHEFRGLWAYIAGPLI 240
            ++   GEL G+ VGMTI+LNVFVAGPISGASMNPAR+LGPAIV   ++G+W YI GP +
Sbjct: 181 TDSRATGELAGIAVGMTIILNVFVAGPISGASMNPARSLGPAIVMGRYKGIWVYIVGPFV 240

Query: 241 GALAGGFVFDLV----KP-RELTRSSSCL 259
           G  AGGFV++ +    KP RELT+S+S L
Sbjct: 241 GIFAGGFVYNFMRFTDKPLRELTKSASFL 269

BLAST of MS019136 vs. TAIR 10
Match: AT5G37810.1 (NOD26-like intrinsic protein 4;1 )

HSP 1 Score: 306.2 bits (783), Expect = 2.5e-83
Identity = 159/269 (59.11%), Postives = 205/269 (76.21%), Query Frame = 0

Query: 1   MASKIDGVEEDKISRLEEG-----TIAVRVARLCPASIVACIQKFMAEVIGTYFVIFAGC 60
           M+S  D +EE++ISR+E+G        +        SIV   QK +AE+IGTYF++F+GC
Sbjct: 1   MSSHSDEIEEEQISRIEKGKGKDCQGGIETVICTSPSIVCLTQKLIAEMIGTYFIVFSGC 60

Query: 61  GSVAVNKIYGGSVTFPGICVVWGLIVMVMIYTVGHISGAHFNPSVTITLAIFRGFPWWQV 120
           G V VN +YGG++TFPGICV WGLIVMVMIY+ GHISGAHFNP+VT+T AIFR FPW QV
Sbjct: 61  GVVVVNVLYGGTITFPGICVTWGLIVMVMIYSTGHISGAHFNPAVTVTFAIFRRFPWHQV 120

Query: 121 PIYIVAQVSGSLLASCTLDLVFDVTVEAFYGTVPAGSNVQSFVLEIIITFLLLFVVF-AT 180
           P+YI AQ +GSLLAS TL L+F VT EAF+GT PA S  ++ V EIII+FLL+FV+    
Sbjct: 121 PLYIGAQFAGSLLASLTLRLMFKVTPEAFFGTTPADSPARALVAEIIISFLLMFVISGVA 180

Query: 181 SNNTGVGELGGVIVGMTILLNVFVAGPISGASMNPARTLGPAIVRHEFRGLWAYIAGPLI 240
           ++N  VGEL G+ VGMTI++NVFVAGPISGASMNPAR+LGPA+V   ++ +W YI GP++
Sbjct: 181 TDNRAVGELAGIAVGMTIMVNVFVAGPISGASMNPARSLGPALVMGVYKHIWVYIVGPVL 240

Query: 241 GALAGGFVFDLV----KP-RELTRSSSCL 259
           G ++GGFV++L+    KP RELT+S+S L
Sbjct: 241 GVISGGFVYNLIRFTDKPLRELTKSASFL 269

BLAST of MS019136 vs. TAIR 10
Match: AT4G18910.1 (NOD26-like intrinsic protein 1;2 )

HSP 1 Score: 248.1 bits (632), Expect = 8.1e-66
Identity = 130/238 (54.62%), Postives = 175/238 (73.53%), Query Frame = 0

Query: 34  VACIQKFMAEVIGTYFVIFAGCGSVAVNKIYGGSVTFPGICVVWGLIVMVMIYTVGHISG 93
           V  +QK MAEV+GTYF+IFAGC +VAVN  +  +VT PGI +VWGL VMV++Y++GHISG
Sbjct: 48  VPFLQKLMAEVLGTYFLIFAGCAAVAVNTQHDKAVTLPGIAIVWGLTVMVLVYSLGHISG 107

Query: 94  AHFNPSVTITLAIFRGFPWWQVPIYIVAQVSGSLLASCTLDLVFDVTV-------EAFYG 153
           AHFNP+VTI  A    FP  QVP Y+++QV GS LA+ TL L+F +         + F G
Sbjct: 108 AHFNPAVTIAFASCGRFPLKQVPAYVISQVIGSTLAAATLRLLFGLDQDVCSGKHDVFVG 167

Query: 154 TVPAGSNVQSFVLEIIITFLLLFVVF-ATSNNTGVGELGGVIVGMTILLNVFVAGPISGA 213
           T+P+GSN+QSFV+E IITF L+FV+    ++N  +GEL G+ VG T+LLNV +AGP+SGA
Sbjct: 168 TLPSGSNLQSFVIEFIITFYLMFVISGVATDNRAIGELAGLAVGSTVLLNVIIAGPVSGA 227

Query: 214 SMNPARTLGPAIVRHEFRGLWAYIAGPLIGALAGGFVFDLV----KP-RELTRSSSCL 259
           SMNP R+LGPA+V   +RGLW YI  P++GA++G +V+++V    KP RE+T+S S L
Sbjct: 228 SMNPGRSLGPAMVYSCYRGLWIYIVSPIVGAVSGAWVYNMVRYTDKPLREITKSGSFL 285

BLAST of MS019136 vs. TAIR 10
Match: AT4G19030.1 (NOD26-like major intrinsic protein 1 )

HSP 1 Score: 220.3 bits (560), Expect = 1.8e-57
Identity = 114/238 (47.90%), Postives = 162/238 (68.07%), Query Frame = 0

Query: 34  VACIQKFMAEVIGTYFVIFAGCGSVAVNKIYGGSVTFPGICVVWGLIVMVMIYTVGHISG 93
           V  +QK +AE +GTYF++F GC SV VN      VT PGI +VWGL +MV+IY++GHISG
Sbjct: 51  VPFLQKLIAEFLGTYFLVFTGCASVVVNMQNDNVVTLPGIAIVWGLTIMVLIYSLGHISG 110

Query: 94  AHFNPSVTITLAIFRGFPWWQVPIYIVAQVSGSLLASCTLDLVFDV-------TVEAFYG 153
           AH NP+VTI  A    FP  QVP Y+++QV GS LA+ TL L+F +         + F G
Sbjct: 111 AHINPAVTIAFASCGRFPLKQVPAYVISQVIGSTLAAATLRLLFGLDHDVCSGKHDVFIG 170

Query: 154 TVPAGSNVQSFVLEIIITFLLLFVVF-ATSNNTGVGELGGVIVGMTILLNVFVAGPISGA 213
           + P GS++Q+F +E I+TF L+F++    ++N  +GEL G+ +G T+LLNV +A P+S A
Sbjct: 171 SSPVGSDLQAFTMEFIVTFYLMFIISGVATDNRAIGELAGLAIGSTVLLNVLIAAPVSSA 230

Query: 214 SMNPARTLGPAIVRHEFRGLWAYIAGPLIGALAGGFVFDLV----KP-RELTRSSSCL 259
           SMNP R+LGPA+V   ++G+W Y+  P +GA+AG +V++ V    KP RE+T+S S L
Sbjct: 231 SMNPGRSLGPALVYGCYKGIWIYLVAPTLGAIAGAWVYNTVRYTDKPLREITKSGSFL 288

BLAST of MS019136 vs. TAIR 10
Match: AT1G31885.1 (NOD26-like intrinsic protein 3;1 )

HSP 1 Score: 213.0 bits (541), Expect = 2.9e-55
Identity = 108/226 (47.79%), Postives = 154/226 (68.14%), Query Frame = 0

Query: 30  PASIVACIQKFMAEVIGTYFVIFAGCGSVAVNKIYGGSVTFPGICVVWGLIVMVMIYTVG 89
           P   V+ +QK + E +GT+ +IFAGC ++ VN+ YG  VT PGI +VWGL+V VMIY++G
Sbjct: 35  PLVSVSFVQKLIGEFVGTFTMIFAGCSAIVVNETYGKPVTLPGIALVWGLVVTVMIYSIG 94

Query: 90  HISGAHFNPSVTITLAIFRGFPWWQVPIYIVAQVSGSLLASCTLDLVF-------DVTVE 149
           H+SGAHFNP+V+I  A  + FP+ QVP YI AQ+ GS LA+  L LVF        +  +
Sbjct: 95  HVSGAHFNPAVSIAFASSKKFPFNQVPGYIAAQLLGSTLAAAVLRLVFHLDDDVCSLKGD 154

Query: 150 AFYGTVPAGSNVQSFVLEIIITFLLLFVVFA-TSNNTGVGELGGVIVGMTILLNVFVAGP 209
            + GT P+ SN  SFV+E I TF L+FV+ A  ++    G   G+ +G TI+L++  +GP
Sbjct: 155 VYVGTYPSNSNTTSFVMEFIATFNLMFVISAVATDKRATGSFAGIAIGATIVLDILFSGP 214

Query: 210 ISGASMNPARTLGPAIVRHEFRGLWAYIAGPLIGALAGGFVFDLVK 248
           ISGASMNPAR+LGPA++   ++ LW YI  P+IGAL+G + + L++
Sbjct: 215 ISGASMNPARSLGPALIWGCYKDLWLYIVSPVIGALSGAWTYGLLR 260

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022143298.17.2e-133100.00probable aquaporin NIP-type [Momordica charantia][more]
NP_001380720.12.3e-10271.85aquaporin NIP4-1 [Cucumis melo] >KAA0060766.1 putative aquaporin NIP-type [Cucum... [more]
XP_004149794.11.5e-10170.74probable aquaporin NIP-type [Cucumis sativus] >KGN57839.1 hypothetical protein C... [more]
XP_038882565.13.3e-10171.48probable aquaporin NIP-type [Benincasa hispida][more]
XP_022948505.16.4e-9771.48putative aquaporin NIP4-1 [Cucurbita moschata] >KAG6607007.1 putative aquaporin ... [more]
Match NameE-valueIdentityDescription
P491731.5e-8562.31Probable aquaporin NIP-type OS=Nicotiana alata OX=4087 PE=2 SV=1[more]
Q8W0362.7e-8258.74Probable aquaporin NIP4-2 OS=Arabidopsis thaliana OX=3702 GN=NIP4-2 PE=2 SV=2[more]
Q9FIZ93.5e-8259.11Putative aquaporin NIP4-1 OS=Arabidopsis thaliana OX=3702 GN=NIP4-1 PE=3 SV=1[more]
Q407462.2e-6857.58Aquaporin NIP1-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP1-1 PE=2 SV=1[more]
Q0DK161.1e-6755.00Aquaporin NIP1-3 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP1-3 PE=3 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1CQD33.5e-133100.00probable aquaporin NIP-type OS=Momordica charantia OX=3673 GN=LOC111013203 PE=3 ... [more]
A0A5A7V2N21.1e-10271.85Putative aquaporin NIP-type OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... [more]
A0A1S3C5C41.1e-10271.85probable aquaporin NIP-type OS=Cucumis melo OX=3656 GN=LOC103496839 PE=3 SV=1[more]
A0A0A0LA397.1e-10270.74Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G345890 PE=3 SV=1[more]
A0A6J1GA143.1e-9771.48putative aquaporin NIP4-1 OS=Cucurbita moschata OX=3662 GN=LOC111452164 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT5G37820.11.9e-8358.74NOD26-like intrinsic protein 4;2 [more]
AT5G37810.12.5e-8359.11NOD26-like intrinsic protein 4;1 [more]
AT4G18910.18.1e-6654.62NOD26-like intrinsic protein 1;2 [more]
AT4G19030.11.8e-5747.90NOD26-like major intrinsic protein 1 [more]
AT1G31885.12.9e-5547.79NOD26-like intrinsic protein 3;1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000425Major intrinsic proteinPRINTSPR00783MINTRINSICPcoord: 114..133
score: 31.4
coord: 226..246
score: 33.45
coord: 159..177
score: 36.0
coord: 77..101
score: 47.18
coord: 189..211
score: 39.62
coord: 38..57
score: 33.55
IPR000425Major intrinsic proteinTIGRFAMTIGR00861TIGR00861coord: 42..243
e-value: 1.7E-53
score: 179.6
IPR000425Major intrinsic proteinPFAMPF00230MIPcoord: 34..243
e-value: 2.4E-54
score: 184.5
IPR000425Major intrinsic proteinCDDcd00333MIPcoord: 38..246
e-value: 1.50824E-57
score: 181.683
IPR023271Aquaporin-likeGENE3D1.20.1080.10Glycerol uptake facilitator protein.coord: 7..255
e-value: 1.3E-68
score: 232.6
IPR023271Aquaporin-likeSUPERFAMILY81338Aquaporin-likecoord: 35..251
IPR034294Aquaporin transporterPANTHERPTHR45724AQUAPORIN NIP2-1coord: 18..250
NoneNo IPR availablePANTHERPTHR45724:SF6AQUAPORIN NIP4-1-RELATEDcoord: 18..250
IPR022357Major intrinsic protein, conserved sitePROSITEPS00221MIPcoord: 95..103

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS019136.1MS019136.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016020 membrane
molecular_function GO:0015267 channel activity