MS019122 (gene) Bitter gourd (TR) v1

Overview


Name	MS019122
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	2-isopropylmalate synthase
Location	scaffold20: 1696418 .. 1712040 (+)
RNA-Seq Expression	MS019122
Synteny	MS019122

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAATACCAACAGCATTTTCAAGGCCGAATCACTATTTTGCTCGGCAATCGAACTCGCAATTTCCTACCTGTCGTCAAATTTACGGTCGGAACTTACAGTTCGGCAATGCCGTCGATTCGTGTCCATTATCCTTCAGGTCGGCGGCGGGCCGTGTATCTCGTGTTTACTGTTGTCAGGCGCAGAGCATAGCTTCCGATTCCAAACCTAATTCATCTGTTCGACGTCCGCCGTACATCCCCAATCGCATCTCTGACCCTTCCTATGTTCGCATTTTTGATACGACTCTCCGCGATGGCGAGCAGTCCCCAGGTGCGTCCCTCACCTCTAAAGAGAAGCTCGACATTGCTCGCCAGCTCGCCAAGCTCGGCGTCGACATAATCGAGGCTGGATTCCCAGCTGCTTCGAAGGAAGATTTTGAGGCTGTCAAGATCATTGCCAAAGAGGTCGGCAATGCCGTCGATCAGGACGGGTACGTGCCTGTAATTTGTGGCCTCTCAAGGTGCAATGAAAATGATATTCGAACTGCATGGGAGGCTGTGAAGTACGCTAAGAGACCGCGGATTCATACGTTTATTGCAACCAGCGAAATTCATATGAAGCATAAGCTGAGAAAGACGAGGGAGCAAGTGATTGAGATTGCTCGAAGTATGGTGCGGTTTGCACGAAGTTTGGGTTGTGATGATGTTGAGTTCAGCCCCGAGGATGCTGGCAGGTTTGATCTTGATCCTTTTGAAAATTTTAAATTTTGTTTCATCTAATTGATCCAAACGAAACAGTAAGCTGACTTTTTCAAAACCTCTCAAGTCGTTATTCAGTTTAGGTTTATGAATCCTGGAGTTGTGAAGTGTGAATTTGTGTTTGTATTGTGTAAAATAATAGGATATGAAAATTTGAACTGATGTAGTTTTTTTTGTTTTTTGATTTGTAAAAGTTGGGGGTTTCAAAAGCTTGATTTGTATAATTTTGGTTTACATAGGCTAGTGTGGTTAGCAAACCTTTAGCTAGTTTGAGCCATTTTGTTGGGCTGGACCTAAGAAGCACGGATACGACACGGACACAGTGATATGTTATTTTTTAAAAATATAAGACACGGACACGGCAATGATACATTTATTAAAATATACCATGTTTTTAGGTGTATATATTTTTATACTGGAAGGAAATTTAAAGTCAACAAGCTTATAACGCAATATTATGTTAAAAAAAAATAGTATTATGCAGCTTACTCTCAAAATTTATTATTCTGTTTTATATTACTATTTATGTCTATTTCTTATGCTTAACAAGTGTTTGATATTACTATTTATGTCTATTTCTTATGCTTAACAAGTGTTTGATATGTGCCTAACAAATGTAGAAACTATTTTCACTTTGTACAACTAGTGTGTGACACACGTCTATTAAGCTAACAAGTGTCCAATAAGTGTCCAAATTGGGACATTGCTTAGACCCTATAGTTGTTATGTAACTTGTAGGTGACCTATTATAGATAGGTTGGAATCATGGTTTGAACCTGTAACCTAAGCCACTTTACTCTAATTAAATAAAATCCAATTAAAATAATTAAATAAAATCCAATTAAAATTTTGGTAGAATGAAGGATACTAATAGAGTTCCCTTTATGCGTCCTTCCATTTGCTTAGACATTATGAAAGTCTAGTTTTTAATTAAAAAAAAAAAAGAAATTGCTTCAGACTTCTAACAGCCATCCCAAAAGAATTATGCTAAAAGAACAAATAATTGCCTATATATTTGATGTCCTATTTATCTGTTGCTCTTCATGAACGATATGTATCTTGTAAAATAATGAAGATAAAATATTTATTTTCTTCTAGACATTACAAATTTATTTTTGCAACTTTTCAAATTTTCGTACATATAAATATTTCCCCTTTTCATTTTCTGGTGTCCTATCAGATCTGATAGGGAATTTCTTTATGAAATTCTGGGTGAAGTTATAAAGGCTGGGGCAACTACTCTCAACATACCAGATACTGTGGGTTATACCATGCCGGTTGAATTTGGGGCATTGATTGCTGATATAAAATCTAATACGCCTGGCATTGAAAATGTCATCATTTCAACACACTGCCAGAATGATCTTGGACTCGCAACTGCCAACACAGTAGCAGTAAGTAATGGCTTTTTCTTCCCTTTTAATCCTGTTGATTGAACTGGATGTTTGATTTGGGAACTACCCGAAGATAGTAGGTAAGTAAAAACGATTACAAATATCATACTATAACACTAAGAAAGAAAAGGAAAAAAAAAAAAACAAGACGTGAAAACGATGAGGCTGCATTTGTTGGAAATACTTATAGTTATGGTCATATTTTAAGGATTTATATGCTTAAAATTTTGCACCTTAAATTATGAAGAAGGGATAAGATATACATTTTCTGCCTTGATCTTGGTCCCTCAAAGTACAAAAGTTGTGGAGGACAAACTTCTTCAGCCGTTCCTGGAAATTTGGCGAATTGCTAAGGGATAATAGTGCGAACTATAATCACTCTTCCACCTATCTAAATGCTGTACGACTTCATCTATTGCCTTTAAGTAAATTATGTTTGATTCCTCTCATCTTTTTTCAGGGAGCATGTTCAGGTGCAAGGCAAGTTGAGGTCACAATTAATGGCATTGGCGAGAGAGCTGGAAATGCTTCGCTGGAGGAGGCAAGTTATTTATTTAATTTAATTTATTTTTATTATTTCATTTATTCTGAAATAATAATGATATTAAATTACAAGTTCGTCCTTCAAGAAGGTTGTGTCTGATAACAAGTTTCTAAACTTTGATAAATGTCTAACAAGCTACTAAACTTTTAATTTTATATCTAATAGGTTCTTGAACTTTCATTTTTGTGTCTACACTGGCTTCTTTGACTATATTTTTCCACAATAATTTAGCACCAATTTTCATCCCATGACCACACCACATAGCAACATAAAAACGTTATTTCAAAGCTTTCCTCTCCAAATACAAATTGGAAACAATAACTATAATTTGAAAATAGATACCACTAACAATATATGGTTAGCCATAGCTTCCTCTTGGAGCTCTTGCTCAGGGGAGTTTTGTTATTATTCCCCTAATGTTGCTCCATATTGGAGTGTTTTTTAAGCTCCTTTGGAGTTCTTGTACATTCTTTACATCGAGAAGAAATGTTTTGTCTCTTATCAATCTCTAAAGCACAATGTTGGCCTTGTTCTTTTCCTCTCCTCCTCCAGCCCAACAATCTGCTACTTTCTTTTAATTTTTTTGGACAGGAAAAAATTTCATTGATGATATGAAATTATACAAAATGATCAAAACCTATCCAGTGAAGTAAAAAAAAACTTCTCCGGTTGGATATAAGAGAATTCAAGCTTTAGTTGCTAAAAGGAGAGATATTTTTACACCATGTTAGAGCCAAAAAAGAATAAAATCAATCAAGTGTTAAGTAGAATTTCCTTGTCCTTAAAAAGCCTAGAGGTTCTTTCTCTCTAAATGGACCAAATGTATGCTCTGTTAAGGTGTTGCCACATGATTACCTTTTCGTGCTTGAAGGGCTGGCTGGCGAAGGAAAATTGACTTGGTAAAATCGTCGCTCAGCCAAAAATGGATTAGAAATGCTTCCAAATCACTGTAGAGAAGGGACAATGAACAAAAATACGAAATTGTGATTCAAACCAACTTTGCATAAGCAGCACCAAGTGGGAGAAATAGACCATTAGGGGCATCCGTTTGTCAAAAGTATTGAGGCAAGAGTGACTTAATTCCCAGATTAGGAACTTGACCTTATTTGGATACATGGCCTTCCAAATGGACCTTATCTTCCAAATGGATTTATTGCGGTTATCAGTGGAGGAAACCGAAAATGTTGGAAGGTTTTGAGTCAATGATTTTGCTGTGAAGCTGCCATGTTTCTTCAGGTTCCATAGCCATAAATTCACTCTATTTGATGGAGTAAAGGAGGACAGTAGGGCTGATAAAGTTGCCCATTCTGCAATTCCTCCTCTTTGGGATTTATTTGAAAATTCATAGTCCAAGACCTATTTGGCTGGAGCTCCCTTTCACGGAAGAAGAAATCTATAAAGTGGTTTGCTGTCTTGGCAATGATAAAGCCCCAGGGCTGGATGGTTTCACTTCTGAATTTTTCAAAAAATAATGGAACATTATCAAATCTGATATTATGACAATGTTCCAAGATTTTTTTAAAAAATGGAATCATCAATGCTGCACTCAACAAGACCTATATTTGCCTCATGCCCAATGGAGAGACGCAAAAACAGTCCACGACTACGGATCCATCAGTCTCATACCCTGTGCATACAAAATACTAGCTCGTGCCCTCTCCGAAAGGCTTAAAAAGGTGTTATCCCACACCATCACCGACCATCAATCCATCTTTGTAGAAGGTAGACAGATCCTTGACTCTTCTCTCATTGCAAACAAAATTATTGATGATTGGACGGTGAGAAAAGAGGAAGGATGGTGTTTCAACCTAATCTTGAGAAGGCCTTTGATATGGTTGATTGGGACTTCCTGGATATGGTTCTACACTACAATGGGTTTGGGGTGAAATGGAGGAAGTGGATACAAGCTTGCATTTCCTCTACTAGCTTATCCATTATCATCGATGGAAAACCAAGGGACAAACTCACAGCTACAAGAGGATTGAGACAAGGGGATCCACTGTCCCCTTCCCTTTTCATCATGATCACGGATGTCCTAAGCCGTCTGCTTCTTCATGGATAACACATAGGCTTCCTCACAGGTTTTGCTTTTAGATGTGGTTTGGTTAATATAAACCATCTTCTGTTTGCATATGACACTCTCCCTTTTTCTAAAAAGGACCCCACCATCATAAAAAATCTCCACAAAGTCATCAAGTTCTTTGAAGTTGCTTCGGGTCTCAACATAAATTACAAAAAAATCCAGTGTATATGGTGTCTCTACTAGCCCACAAATGACAGAGCAGATTGCGAAGGAATGGGGTTGTGCTTCAGGGAGTTGGCCAAGCACCTATTAGGGTCTACCTTTTAATAATCAACTTAGCAAATGGAACAACCTCTTTATCTCTAAAGGGGGAGACTCACCATCCTCCAGTCAGTCCTCACAAATTTACTAATCTACCACCTCTCCCTTTTCAAACTATCTGGAAAAGCTGCCAAGCATATAAGCAAAATTTTCAGAGATTTCCTATGGGAAGGACCAAGTTTAAATGGCAAGCCACATCTTATAAATTGGACCTTCACACAACTTCCACTACATGAAGGAGGTCTCGGTGTTGAGAACATCAAACAAAGGAACCGAGCCTTACTATCCAAATGGATTTGGACATACATTACCGAGCCAAACCAATTGTGGAGGAAGCTCATCACGGCAAAATACACTGGTTCTTTCTCGATTTCTTGGCCAACCATTCGCTCCAACTTTTATAAAGGGCCATGTACTGGAATCATACGACAAACCAGCATTATTAAGACTTATGCAACCAGCAGAATGGGGGAAAGAAGTCATCTCAACTTTTGGCATGACAGGTGGCTCACACAAGGAACCTTGGACAGCCACTTCCCGAACCTTTTTGTCATATCATCAAACAAAAAGGCCACAATTTCAGATTTATGGTTGAAAACTACATCCTCCTGGATTATAGTCTTTGAAGACCTCTTAAGGATGAGGAGTTTATAGATTGGTTGTGTCTCTCCCACATCCTAAATGAGATTACCCCCACATCCCTTGATGACCTATGGATTTGGCCCCTAAAAGCAAATAGATCCTTCTCCACCAAATCGGCTTATCGAATCCTCACTACCAATAGCTCCCTCACGGCCTCCACCTTCTTCCCACGCATTTGGCAAGGAAAGTGCCCTAGAAAAGTAAAATTCTTCTGCTGGGAGCTTAGTCAGGGGGCGATAAACACCCATGATATGGGTCAGAGAAAGCTCCCACACTTTGCCCTCTCCCCTAATTGTTGTCACTTATGTTATGGAGCACCAGAGACTCAGGGCCATCTTTTCATTCATGTGCATATGCTACCCGTTTTGAATTATCTCCTCACTGCTTTTGGGTGGAAGACAATTCTACACAACAAACCATCTACCTTCCTTGATCAATTATTGGCGGGACATCCATTCAAGAAGGGAAGAGCTACCCTTTGGGTTCAGTTTGTTCAGGCCTTTCTTTGGAGCTTATGGTTGGAGAGGAAAAATAGGGTCTTTATTGAAAAAGCCCTTCCTTTTGATATCTTTTTTGATAGAGTTACTATTCTAGCACTTGGTTGGTGTAAATGTTTCTACCCCTTCAGTAGCTATTCTCTAAATGAACTTATTACAAGTTGGAAGACTTTCTTGTAATTGCCCATGTGGCTTTTTTGTATATTTCATACCATCAATGAAATATTTGTTTCATATAAAAAAAATACCTATTTGGCTCGTTCCATCAATTAGCAACTGATGCATTCGTTTTAATACTTAGAGAATGTAATAGTGGGAATTCCATCATTAGTGGTCTGTTCAATCCAGTGATCTGTCAAGAAACAAAGTAGTGCCACCATTTCCAACTTTGCAGCGAATATTTGAGAAGATGAGTTCTTACTGTTATTGATGGATTCCATGGTCCTTTTGTTGAATGAAGGGATATAGCATCATTCTTTAGGTTGAAATGCTCGGTTCCATATTTGGCAGTGATGGTCTTCCTCCATAGAGCTTCCTTTTCTATATAAAACCTCCTCCACTTTGCTAATAGGGCTTTGTTCCTTTGTTTAAGATCAATGATGCCTAAACCACATTCGGATAGTGTGGGGGTTTCACTAGGTTCCAATTGCGGAGATGAATGCAATCTTTTTCCTTATTGCCCAACCAAAAAAGCTTTCTATAGGTGTGCTCTTGGAGGCAGCAACTTTGGAAGGCATTTGGAAGAGTGATGAGAAATAAATAGGAAAGTTATCAAGGGTTGCTTGAATAAGAGTAAGCTTACCTTCTTTAGATACAACATAGGAGTGGTTCAAACTTTGTATTCTCAACTCCACTTTTTGAAGGAGGGGTTGCCAAGGAGGAAGGGCCTAAGTATGAGCTCGGCCAAGTGCTCGCCTTACACCCAATCAAATTGGTGAGATGGGAAATGTGATCATCTTCACAGCATATCCCCAAAAACTCAGATTTGTGGTGGTTGACGTTAAGATCCGAGGCTTCTTCAAAGCGATTGATGCAAGAAAATAAATTTTGAATGTCTTTCTCCTCATGAGGAAAATAAAATGGTTCGTTAGCGAACTAGAGGTGGATGATTTGCAACCTTTCTTTACCCACATGGAAACCTAATGAAGTTATTTGATGCTGTAAGAGATAAGATTCTGCTTATTTTTTTTAGATAAGAAACTTATTTGTATTGATCAAAAAGGGGAACAAACTAGAGGCTGGGGTAGAGGAAACCCCACCAAAAAACTAAACAACCGCTTTCCAATTGTGCATAAACATGGAAAGGTTCAAAAGAATTTGCTATAATTGATACACCACCAAGAGACCGTGTGCTGTATGTCACAATCGCACCTTTTCAAAGCAGCTCGAGCGATTGTGCGGCACTTGCTCTAGCTCAAACGAGCAAGTCAGCCGAGAAAAACTGTAAGTTGCAGGCAATCTCGGTCGTGGGTCTAGCCTCCGCTCACGTTTTACGAGAAAATTGGTTTTAGAAAACGTGAGAGAGAAAAACAGTACATCTTTAAAACAAGCATTGTAGACATTGGATGCAAGAAAGCTTTGATTGAAAGAAAGTATACAACATTTGGTCGGGGGGAGGTTTGATGACTACTTGGTCCCCCCTAAGGTACATTATAAGGCATTTTTGGTGTGGCAGGTGTAGACGTCCTAAGTGGCGAGGAGTCACTCGGTGGCCTACAGGGCCCGTGTGAACTCCGATTGGCTTGAAATTTGGCGTGCTTACATATCTTACTACTGCACACCTAGTTCCAAAGCCGCTCGGCCAAATTCCACCTACAACACGAAAAACTCGATACAAGAAAATTACACTAGAAAGCTTTAAAGACATGAAAGAAAAGCGAGTGGTGAGGATGGTTTGGACATGCGGCCATGGGTAACACACCCTGGACAAGCATGCCCACAACACTGTACATCCCTACAAAAAAAACCAAAAGTAGTGGACTTATCTTCAAACATGTGAGAATTTCTTCCAGCCAAATATTCCAAAGGAGAGCTCTTGAGGCGCACCTCCAAATAAATTTAGTCTTCTTAGGGAGAGTCCACCCAACAATCGAAATAGAGAGGAAGGGAATAAGAGGGTCTTCTTATTTAAGACCCCTTGTGGCACAAATTTTTCCTCGGGCCTTGCTATCTATAATGATGGGAAAATTTTAGAATCACATCCTTAACACCTTTGGATGGTCCACTGCCTTGCCAACTGATCCACCACTCCTCGAAGTTACTCTTGCAGGCCGCCCTTTTAGAAAGGAAAAGAAAATTCTATGGCAGCACTTCATTCAAGCTTTCTTTTGGATCATATGGAGAGAAAGAATTCAGCGCACCTTCAACGATAAATCTCTCTCATTCGACAGACTTTTTAAAGCTCTTTCTAGTTATTACATGGTGTAAATGCTCTCCATTGTTTCATCACTATAGTTGCACTTACCTTTTAGCCAATTGGAAGTGCTTCATGTAATCCCCTTTCTGGACTTTTGTAATTTCATACATCAATGAAATTGTTTCTTTCACAAAGGAAAAAAAAAAAAAAAAGAAAGAAGAAAAAAATCAGAAGAGATGACCCCTTGATCCATTGCCTTCAAGAATCCCTAAAGCCTTTTGCTTCCAAGTTGTAAAGACTAAGGTTGGAGAGAATCCAATGGAATGTATTCTCATTATTAAAGTCTATATATATAGACAAATATACAAAGTTGACCTAATCCTATAAGGAGAAGGTAAAGGACAAATTAATATTAATACAAGAATATAAAATATACTAATATCTATACTCTAACACTCCCCCTCAAGTTGGTGCATATATGTTAATCATGCCCAACTTGTTAGACAAATAATCTATTCGTGCTCTGTTATGTGATTTTTGATATCATTGGGATCACCTCAGAAGTTACAATTGATTTCTTCTCAGCCATAACAAACAAACGAACTTATTTGAACAAAACGACAGAACCAGACCAAACCACCCGAACCAAACAAAACAGATGAATCAAATTGCTGGAACCAAACCGAATTAGGTGTTTTCTGAACCCAGAAACCCTAAGTGCTACCCCACGAGAACCAGAGGGGCGAAGGCGACGGGTGGCGACGAGACGAAACCCATGAACAGCAATCGGAAAACAGCAGTGCTGCCGAATCAGCAAACGTCGGCGGTCGGCGGCGGCGGCGGTGAGTTGTGTTGGATAGCAATGAACAAAACCCTAAGGCACAAAACTCTAATGGGCATCAAAACCCTAATGGGCACAAAACCCCTAATGGGCACAAAACCCTAATGGGCATCGAAATCCTAATGGGCACAAAAAGTTTAGAAGAAACCTAGAGCTCTGATACCATGTAGAGACTAAGGTTGGAGAGAATCCAATGTAATGTATTCTCATTATTAAAGTCTATATCTATAGACAAATATACAAAGTTGACCTAATCCTATAAGGAGAAGGTAAATGACAAATTAATATTAATACAAGAATATAAAATATACTAATATCTATACTCTAATACAAGTCAAGCTTTATAACCACTCCATTTTTCTTTTTTCTAAACCATTTGTCAATTATTTCATTGCCTATAAGGGATGTGTCTAGAATTTGCCTTCAGCAACAAAAGCTCATTGATATTCTATGATGATACTCGACAACACTTTTTTCAACCTTTGGATAGTACGCTTTTTTAACAATTGCAGCTTTCCAATCATTGTAGGCTATAACAAGTATTCCTTGTGGGTTTCTAAGGTTTCTATAGCTTTTTTCTGACCTTTGAGCTTCTCAATAAAACCACGTCCAAGCCATCCTCTAAGGGGGTTGTTTTTCCACCAGAAATTGTGGATACCAATTGTCAACATTATAGGATAATGGTCCGAGGTTGTCCTCTCAAGCTTTTCTTAACCGATGCTTAAGAGAATTTGGTGATGATGGACTCTAATAGAGGAAACGGTCAATGAGGATTAGTGTAGGTGAGTATCTGAAATCGGACCAAGTTACCCTTCCATCGGAAAGAGCTACATCTATGAGCCCGCCTGATTGATGAAGGTGTTGAACTTTCTCATAAACTTGGAGTGTCTTTTGCTTGAAGTATCTATTTTTGAATATTATAGGTAATAACTTATCAAAGTTGTTTTAACTTTGTTTTACTAGAGTTTTTCTTTTATTTTATTTCATGGACATATCTGAGGATAAATAGTCTATCCGTCATTCTACAATACTATTATTTCTTGTATGTAAAAGAGGATGAATATTAAATTTAGTGACAATAGTGTCAATTTTGTACAACTGTTCTTATCTAATTTTTTTTTATACAACTGTTTTCCTTTATGAGTTAGATGTGACTGAATGCATGGTGCTGTTGAAGTGTTTTGAGAACATCTGTTTGCGCTGACATGTGTAGGTAGACTTCTTAAATGACATACATCTATTTTCTTCTTTTAAACCATATTTTTATATCTTCAGGTGGTAATGGCCTTACAATGTCGTGGAGAGCATGTGCTTGGAGGTCTTCATTCTGGAATTAACTCGAGACATATCTTTCTGACCAGCAAGATGGTAATTATTTTTCTTACAAAGAAAAGCTTTTTGAAACTCAAGTTAATTTTTAAAAGAAAAATTATTCTTGTCATCCTTTTTTTTTAAAATATTTTTTTAATAAAAAAACGTACAAAGTTAAACGTTCCATAGGGCAGGATATTTCCTGTTGCAAGTTGTTTGTGGCCCTAGGTTGAACTTGAAAGCTAGACAGTTTTGTTCTTGCTTCTAGCTCCTCTTTGGAAGACTTGGAAAGAGAAGAAGGGGCAAAGTTTTTGAGGATGGGAACTGGGAAGAGTGTTTCTTTCTTTTTTTTTTTTGGGGGGGGGGTGGGGTGAGAGGTTTAATGATCCAACGTCCAAAGTCCTATTTATTTTTTATTTTTTTCATGACTATTGTTTCTTTTTAAAATCTGTTGGTTAGAACCGTTTTTGGTTTTTTTTGTTGGGGTTTAGTGTACTTCAGATTTTGAATTAACGACAGATTGCTGGTTCTAGTTCTCTCTTCGCCTAGTTCACTTTCAATTTTTCTATTCAAGTTTCAACATCCGTATTTCTTATTAATAAAAGGCTACTTACGGACAAACTTATTCCTCAGGTAGAAGAATATACTGGCTTGAATGTACAACCGCACAAGGCTATTGTTGGAGCTAATGCTTTTGCTCATGAAAGTGGCATCCACCAGGTTAGACTTGTTAGATCTTGATATGCTAGGTTTTGTTGTTCATGGCTAACTATTATGACGTGTTTTCATATTTATCTTTCAAAATCCAGCGTGATGTGAAAGCTTCCAAATATACTCTCATAGCTGACTATGTCAAGGTTCTAGCATCAGTGTTTATTGCTTTAAACACATTTAAAGAAAACAGTTCGCCATTTTTTCCTTTATTTCACCTCTCCCCTGTGAGTTAATTGAAAAAAATTTCTTTTTTTAGATTGTCAAATTCAAGAAAGAGAGGTACAGAAAGGACAGCAAGGGAAGATGATTCTTTCCCTATGAGGTGATGGAGATTACATTGGATTCTCCTCAATTCGGGAGAACGATGAGAGAGTGTGAAACACCTTCTAAAGCACTCCAAGAGAAAGGAAGAAAACAGTGTCTTCCTATATAGTGTTAAAATTTCTATCCAATCTGTAAGAAAATTTTCCAATTCCTTTCAAGCTGAGCGCTCTATATACTTGTAGTCTTTTGCTTGAAAGGGTGGCATAGCACTTAAGACCGATCCTCTTCATCCAAAGCCTAGTTGAATACAAGATGCAGCTGGTTGTATCTTGTAGTTTCATCCTGGCCTCCATTATTCTTGTACTTGAGTCATCAGGTGACAGGTATTTTTGGGGCCATAGAATGTATTTAATTGTTCATAAAGAACCAGTTGTTCATTACTAATTACTGTCCGAGGAGTTACATGATTGTTATAGAGCAGCTACGTTCTCTTATAAAAATGGAGGACTCTTTTAACTATTTTGATGCATAGTCTTGCCGTAAAGTTCATAATGAAAATATTGATTGGCTCTTGTTCTATGTTTTTAATAATTATTACTATTTTTGTTTGAGCCATGTGGGTGACTGTTTATCCTGTTTTGTTACGCTCAAATTGTATCTTAAATATTGCTTTTGTTACATAGGATGGGATGCTTAAGCACAAAGGTACTTATGAAATCATATCCCCTGAAGACATTGGCTACGAACGATCCAATGATGCTGGTATTGTTCTAGGAAAACTTAGGTATTCCATGTTGCTTTATGGAAAATGACAATAGTGCTTTTATCTTGACAAAATCTCCCTACCTACCTCCCCTGCCTCCCCTTTCCCATCTCTCTATGTGTGTGTAAGCATGTCTGTGCCTGGTTAGAAGAAGAAGTATTTAGTGCAGTTGTTCGCTGGGATTTATTTTGAGGCTATTTCGGGGGTTGTTTATTAGGGGCTCCGCACGAGGGCCAAAAAAAGGTGGGGGGAGTGAACAGCAGCAGACCATATGAAAATTAGAAAAAAGTATGTGAAAAATATAATATCGGGGCTTTACCTAAGAGATTGTTTAAAAATACTATGCAGCAACTTTTTTCCTTTTATAATAAAGAAGGGAACAATTGAAAATACCCAAAATTTACAAAACCCTAATGGGCACAAAACCCTAATGGGCATCAAAACCCTAATGGGCACAAAAAGTTTAGAAGAAACCTAGAGCTCTGATACCATGTAGAGACTAAGGTTGGAGAGAATCCAATGTAATGTATTCTCATTATTAAAGTCTATATCTATAGACAAATATACAAAGTTGACCTAATCCTATAAGGAGAAGGTAAAGGACAAATTAATATTAATATAAGAATATAAAATATACTAATATATACTCTAACATACAGATATACTGTTTAACAGATATACTGTTCAAGAGAATCCTAGTATGCCATAGAGGTAGAACCTTTCTGCAGCACTTGAAACACCTCTTTGTTGAGATTCCTTTAGTACACGAAGAGGATCTTAGTAACATTGGTAGTATGTTTAATTGCTTAAGAACATAGAAGAGGAGATTGAAGAAGGTATCTTGCAAGAACATGCATAATTTGTAAGGCATTTTCATAAATTCAAGGTCAGATCAATTTTAAGAATATTTGAATACAAATGGAAAAATTGGCCCCTTTTAGCTGTAAAATATCGTATAGTTTGGTACAATGATGCAGCTGTACCTAGGGAGCTGAAGTATATCTGGGACAGGAGGGATGAGGAATATCTGTAGCCATTCTATTGTGGCGTTGAAACTTTAAATTATTTTGTTACAACATCAACAGGATTGACTTCCTTTTGTTTATAATTTCACCCCTTGTATCTGGTCCGAAGTATGCCACCTGCTAACATGGTTCATTTTGACAATTTTTACAGTGGACGCCATGCCCTTAAAAGTCGACTTCAAGAGGTAAATTGGGATCTTCAAGCTCAATGGTTGTGTAATCTTTCTGTATTATGTCTTCCTTTCTGACAATTTGTTTACGTTATATCCCATTTTTTCAGCTTGGTTATGAGCTTGACAATGAGAATCTTGACAACATATTCTGGCGTTTCAAAGAAGTAGCTGAACAAAAGAAGGTAACTTGAAATTTTGTCCCTCTCTGTTTTCTGAGCGAATTCCCTCCTCTGCCTCCTGCACGTATCTGTAAATACAAAAAAGGATTCTACTTTGGGTGCGATCATACCGGTGCATGATCCCATCAGAACTCTGTAGTCAAGTCTGCTTGGGCGAGAGTTGTACTAAATTGAGTGGCTACTTAGAAAGCTTTCATGTTGCACCCCTTTCAAACTTATTAAAAAAAAAAAAAAAGAAGAAGCTCGTACTTCAACCTATTTCAAGAACATAAATTACCATTCATCAGGAAAGTTTTTTCATATAATTTCTTGCCTTTTTCCCTAATTTTCAGATATTTAGATCTACATTATTTTGCTCTAATTTATATGACATTCCAGAGAGTAACAGATGCTGATCTTCGAGCATTGGTATCTGATGAAGTTTTTCAACCAAGTGTTGTCTGGAAGCTGCTAGATTTGCAGGTTTAAATTCTGTTATAGATCATTATATTTGGGAAGTTGTTTGGACCTACTTTTAAATACACATATAGCATCATTTGACAATACCTCAAATGCAGGTCACCTGTGGAACTCTTGGTCTTTCTACGGCTACCGTGAAACTCCTCGATGCTGACGGGAAAGAGCATATTAATTGTGCTGTTGGAACGGGGCCTGTAGATTCAGCTTACAAGGCTGTAGATGGCATTGTGAAGGTTTGTATAGGAGAAACTGCTAGCGAACTTTTTATATTTTCCATTTACATCTGGAATACACTTTCAGGAAGATTTCCTGGTAAAGTCAAACTGGGATATCTTAGATAATTTGTTTTATGTTATTCTCGGCTTCCGTCTAAGAAGTCCCTGAATTTTAGAAGTGTCTAATAAATAGGTTATAACGTAAAAGTCCTCGTGATCGATAAACCAGGCTTTCACATCCTTTGCATGTGTATCTAGTTCTGGGGTATTACTGAATGATTTAACTATGATGTTGTCTTCTTGCCATAAATTTCTTGCTGGTGGTCCAGTGTGTCTTCCTTGAGTGAATCAGACGGTACAAGAGTTATCATTTTTTTTTAATGCAGGAAACAGTTACACTTCTCGAGTATTCGATGAACGCTGTCACTGAAGGCATTGATGCAATTGCCACGACCCGTGTTGTAATAAGAGGGGAAAACAGTTACACATCAACTAATGCAATGACAGGAGAAGCAGTTCAACGAACATTTAGGTAAACTAGCTACAGGATAATGTGATACCTGCTTATTTTCTTTTAAAGGGAAAATGACAAAAACCACCCCAAGATTTTAGGAGTTGTTACAATTGCACTTTTGCACCATCAGTTGATCGGTTACCTACTAAACTTATGAAATTGTTCGAATTAAACCCTTAAATATTACAAAATGCACAAACCACCCTTAAACCCTTAAAACTACAAAGATTGTTACCAACCCCTAGTTGATGGGTGAGTTTTGCAATTTTCCCTTTTTTAAAATACAATCAATCAAGTATTCTTGTTTGAAGTATTATTCCCTCTGCGATATTACATCTAAACGTCATTAATATTCAACTCGAAAAGATTTGGGATGGAGAATTATCAAATGCTATATCAATCAATCCATATCAAAGAACTTCAAAGATGAATCTATTAACTTTTCCATATTCTTTGCTACACACTAGCAAGCTATAGCATATCGATAAATGTATGTCTAGTGATCTGTTTTTTATCGTTGCAGTGGTATCGGGGCAGGCATGGACATAGTTGTATCAAGTGTCAAGGCCTACATTGGTGCACTAAACAAGATGCTTGGTTTTCAAGGAGTTGACATTAAGGTGACTGAAAAAAGAACATTGTCTGCA

mRNA sequence

ATGGCAATACCAACAGCATTTTCAAGGCCGAATCACTATTTTGCTCGGCAATCGAACTCGCAATTTCCTACCTGTCGTCAAATTTACGGTCGGAACTTACAGTTCGGCAATGCCGTCGATTCGTGTCCATTATCCTTCAGGTCGGCGGCGGGCCGTGTATCTCGTGTTTACTGTTGTCAGGCGCAGAGCATAGCTTCCGATTCCAAACCTAATTCATCTGTTCGACGTCCGCCGTACATCCCCAATCGCATCTCTGACCCTTCCTATGTTCGCATTTTTGATACGACTCTCCGCGATGGCGAGCAGTCCCCAGGTGCGTCCCTCACCTCTAAAGAGAAGCTCGACATTGCTCGCCAGCTCGCCAAGCTCGGCGTCGACATAATCGAGGCTGGATTCCCAGCTGCTTCGAAGGAAGATTTTGAGGCTGTCAAGATCATTGCCAAAGAGGTCGGCAATGCCGTCGATCAGGACGGGTACGTGCCTGTAATTTGTGGCCTCTCAAGGTGCAATGAAAATGATATTCGAACTGCATGGGAGGCTGTGAAGTACGCTAAGAGACCGCGGATTCATACGTTTATTGCAACCAGCGAAATTCATATGAAGCATAAGCTGAGAAAGACGAGGGAGCAAGTGATTGAGATTGCTCGAAGTATGGTGCGGTTTGCACGAAGTTTGGGTTGTGATGATGTTGAGTTCAGCCCCGAGGATGCTGGCAGATCTGATAGGGAATTTCTTTATGAAATTCTGGGTGAAGTTATAAAGGCTGGGGCAACTACTCTCAACATACCAGATACTGTGGGTTATACCATGCCGGTTGAATTTGGGGCATTGATTGCTGATATAAAATCTAATACGCCTGGCATTGAAAATGTCATCATTTCAACACACTGCCAGAATGATCTTGGACTCGCAACTGCCAACACAGTAGCAGGAGCATGTTCAGGTGCAAGGCAAGTTGAGGTCACAATTAATGGCATTGGCGAGAGAGCTGGAAATGCTTCGCTGGAGGAGGCAAGTTATTTATTTAATTTAATTTATTTTTATTATTTCATTTATTCTGAAATAATAATGATATTAAATTACAAGTTCGTGGTAATGGCCTTACAATGTCGTGGAGAGCATGTGCTTGGAGGTCTTCATTCTGGAATTAACTCGAGACATATCTTTCTGACCAGCAAGATGGTAGAAGAATATACTGGCTTGAATGTACAACCGCACAAGGCTATTGTTGGAGCTAATGCTTTTGCTCATGAAAGTGGCATCCACCAGGATGGGATGCTTAAGCACAAAGGTACTTATGAAATCATATCCCCTGAAGACATTGGCTACGAACGATCCAATGATGCTGGTATTGTTCTAGGAAAACTTAGTGGACGCCATGCCCTTAAAAGTCGACTTCAAGAGCTTGGTTATGAGCTTGACAATGAGAATCTTGACAACATATTCTGGCGTTTCAAAGAAGTAGCTGAACAAAAGAAGAGAGTAACAGATGCTGATCTTCGAGCATTGGTATCTGATGAAGTTTTTCAACCAAGTGTTGTCTGGAAGCTGCTAGATTTGCAGGTCACCTGTGGAACTCTTGGTCTTTCTACGGCTACCGTGAAACTCCTCGATGCTGACGGGAAAGAGCATATTAATTGTGCTGTTGGAACGGGGCCTGTAGATTCAGCTTACAAGGCTGTAGATGGCATTGTGAAGGAAACAGTTACACTTCTCGAGTATTCGATGAACGCTGTCACTGAAGGCATTGATGCAATTGCCACGACCCGTGTTGTAATAAGAGGGGAAAACAGTTACACATCAACTAATGCAATGACAGGAGAAGCAGTTCAACGAACATTTAGTGGTATCGGGGCAGGCATGGACATAGTTGTATCAAGTGTCAAGGCCTACATTGGTGCACTAAACAAGATGCTTGGTTTTCAAGGAGTTGACATTAAGGTGACTGAAAAAAGAACATTGTCTGCA

Coding sequence (CDS)

ATGGCAATACCAACAGCATTTTCAAGGCCGAATCACTATTTTGCTCGGCAATCGAACTCGCAATTTCCTACCTGTCGTCAAATTTACGGTCGGAACTTACAGTTCGGCAATGCCGTCGATTCGTGTCCATTATCCTTCAGGTCGGCGGCGGGCCGTGTATCTCGTGTTTACTGTTGTCAGGCGCAGAGCATAGCTTCCGATTCCAAACCTAATTCATCTGTTCGACGTCCGCCGTACATCCCCAATCGCATCTCTGACCCTTCCTATGTTCGCATTTTTGATACGACTCTCCGCGATGGCGAGCAGTCCCCAGGTGCGTCCCTCACCTCTAAAGAGAAGCTCGACATTGCTCGCCAGCTCGCCAAGCTCGGCGTCGACATAATCGAGGCTGGATTCCCAGCTGCTTCGAAGGAAGATTTTGAGGCTGTCAAGATCATTGCCAAAGAGGTCGGCAATGCCGTCGATCAGGACGGGTACGTGCCTGTAATTTGTGGCCTCTCAAGGTGCAATGAAAATGATATTCGAACTGCATGGGAGGCTGTGAAGTACGCTAAGAGACCGCGGATTCATACGTTTATTGCAACCAGCGAAATTCATATGAAGCATAAGCTGAGAAAGACGAGGGAGCAAGTGATTGAGATTGCTCGAAGTATGGTGCGGTTTGCACGAAGTTTGGGTTGTGATGATGTTGAGTTCAGCCCCGAGGATGCTGGCAGATCTGATAGGGAATTTCTTTATGAAATTCTGGGTGAAGTTATAAAGGCTGGGGCAACTACTCTCAACATACCAGATACTGTGGGTTATACCATGCCGGTTGAATTTGGGGCATTGATTGCTGATATAAAATCTAATACGCCTGGCATTGAAAATGTCATCATTTCAACACACTGCCAGAATGATCTTGGACTCGCAACTGCCAACACAGTAGCAGGAGCATGTTCAGGTGCAAGGCAAGTTGAGGTCACAATTAATGGCATTGGCGAGAGAGCTGGAAATGCTTCGCTGGAGGAGGCAAGTTATTTATTTAATTTAATTTATTTTTATTATTTCATTTATTCTGAAATAATAATGATATTAAATTACAAGTTCGTGGTAATGGCCTTACAATGTCGTGGAGAGCATGTGCTTGGAGGTCTTCATTCTGGAATTAACTCGAGACATATCTTTCTGACCAGCAAGATGGTAGAAGAATATACTGGCTTGAATGTACAACCGCACAAGGCTATTGTTGGAGCTAATGCTTTTGCTCATGAAAGTGGCATCCACCAGGATGGGATGCTTAAGCACAAAGGTACTTATGAAATCATATCCCCTGAAGACATTGGCTACGAACGATCCAATGATGCTGGTATTGTTCTAGGAAAACTTAGTGGACGCCATGCCCTTAAAAGTCGACTTCAAGAGCTTGGTTATGAGCTTGACAATGAGAATCTTGACAACATATTCTGGCGTTTCAAAGAAGTAGCTGAACAAAAGAAGAGAGTAACAGATGCTGATCTTCGAGCATTGGTATCTGATGAAGTTTTTCAACCAAGTGTTGTCTGGAAGCTGCTAGATTTGCAGGTCACCTGTGGAACTCTTGGTCTTTCTACGGCTACCGTGAAACTCCTCGATGCTGACGGGAAAGAGCATATTAATTGTGCTGTTGGAACGGGGCCTGTAGATTCAGCTTACAAGGCTGTAGATGGCATTGTGAAGGAAACAGTTACACTTCTCGAGTATTCGATGAACGCTGTCACTGAAGGCATTGATGCAATTGCCACGACCCGTGTTGTAATAAGAGGGGAAAACAGTTACACATCAACTAATGCAATGACAGGAGAAGCAGTTCAACGAACATTTAGTGGTATCGGGGCAGGCATGGACATAGTTGTATCAAGTGTCAAGGCCTACATTGGTGCACTAAACAAGATGCTTGGTTTTCAAGGAGTTGACATTAAGGTGACTGAAAAAAGAACATTGTCTGCA

Protein sequence

MAIPTAFSRPNHYFARQSNSQFPTCRQIYGRNLQFGNAVDSCPLSFRSAAGRVSRVYCCQAQSIASDSKPNSSVRRPPYIPNRISDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQLAKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNAVDQDGYVPVICGLSRCNENDIRTAWEAVKYAKRPRIHTFIATSEIHMKHKLRKTREQVIEIARSMVRFARSLGCDDVEFSPEDAGRSDREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKSNTPGIENVIISTHCQNDLGLATANTVAGACSGARQVEVTINGIGERAGNASLEEASYLFNLIYFYYFIYSEIIMILNYKFVVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDADGKEHINCAVGTGPVDSAYKAVDGIVKETVTLLEYSMNAVTEGIDAIATTRVVIRGENSYTSTNAMTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKVTEKRTLSA

Homology

[Close]

BLAST of MS019122 vs. NCBI nr
Match: XP_022141881.1 (2-isopropylmalate synthase 2, chloroplastic-like isoform X1 [Momordica charantia])

HSP 1 Score: 1123.6 bits (2905), Expect = 0.0e+00
Identity = 590/659 (89.53%), Postives = 599/659 (90.90%), Query Frame = 0


Query: 1   MAIPTAFSRPNHYFARQSNSQFPTCRQIYGRNLQFGNAVDSCPLSFRSAAGRVSRVYCC- 60
           MA+ TAFS PNH F RQ NSQFPT   IY RNLQ        P SFRS AGRV    CC 
Sbjct: 1   MAMATAFSSPNHCFGRQWNSQFPTSGHIYRRNLQ-------NPSSFRSPAGRV----CCS 60

Query: 61  --QAQSIASDSKPNSSVRRPPYIPNRISDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIA 120
             Q+ SIASDSKPNSSV+RPPYIPNRI DPSYVRIFDTTLRDGEQSPGASLTSKEKLDIA
Sbjct: 61  EAQSISIASDSKPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIA 120

Query: 121 RQLAKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNAVDQDGYVPVICGLSRCNENDIRTA 180
           RQLAKLGVDIIEAGFPAASKEDFEAVKIIAKEVGN VDQDGYVPVICGLSRCNE DIRTA
Sbjct: 121 RQLAKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKDIRTA 180

Query: 181 WEAVKYAKRPRIHTFIATSEIHMKHKLRKTREQVIEIARSMVRFARSLGCDDVEFSPEDA 240
           WEAVKYAKRPRIHTFIATSEIHMK+KLRKTRE+VIEIARSMVRFARSLGCDDVEFSPEDA
Sbjct: 181 WEAVKYAKRPRIHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFSPEDA 240

Query: 241 GRSDREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKSNTPGIENVIISTHC 300
           GRSDREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIK+NTPGIENVIISTHC
Sbjct: 241 GRSDREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVIISTHC 300

Query: 301 QNDLGLATANTVAGACSGARQVEVTINGIGERAGNASLEEASYLFNLIYFYYFIYSEIIM 360
           QNDLGLATANTVAGAC+GARQVEVTINGIGERAGNASLEE                    
Sbjct: 301 QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEE-------------------- 360

Query: 361 ILNYKFVVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAH 420
                 VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAH
Sbjct: 361 ------VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAH 420

Query: 421 ESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNEN 480
           ESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNEN
Sbjct: 421 ESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNEN 480

Query: 481 LDNIFWRFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLL 540
           LDNIFWRFK VAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLL
Sbjct: 481 LDNIFWRFKAVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLL 540

Query: 541 DADGKEHINCAVGTGPVDSAYKAVDGIVKETVTLLEYSMNAVTEGIDAIATTRVVIRGEN 600
           +ADGKEH NCAVGTGPVDSAYKAVDGIVKE V LLEYSMNAVTEGIDAIATTRVVIRGEN
Sbjct: 541 NADGKEHTNCAVGTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGEN 600

Query: 601 SYTSTNAMTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKVTEKRTLSA 657
           SYTSTNA+TGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKVTEKRTLSA
Sbjct: 601 SYTSTNAITGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKVTEKRTLSA 622

[Close]

BLAST of MS019122 vs. NCBI nr
Match: XP_022141882.1 (2-isopropylmalate synthase 2, chloroplastic-like isoform X2 [Momordica charantia])

HSP 1 Score: 1108.6 bits (2866), Expect = 0.0e+00
Identity = 582/651 (89.40%), Postives = 591/651 (90.78%), Query Frame = 0


Query: 1   MAIPTAFSRPNHYFARQSNSQFPTCRQIYGRNLQFGNAVDSCPLSFRSAAGRVSRVYCC- 60
           MA+ TAFS PNH F RQ NSQFPT   IY RNLQ        P SFRS AGRV    CC 
Sbjct: 1   MAMATAFSSPNHCFGRQWNSQFPTSGHIYRRNLQ-------NPSSFRSPAGRV----CCS 60

Query: 61  --QAQSIASDSKPNSSVRRPPYIPNRISDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIA 120
             Q+ SIASDSKPNSSV+RPPYIPNRI DPSYVRIFDTTLRDGEQSPGASLTSKEKLDIA
Sbjct: 61  EAQSISIASDSKPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIA 120

Query: 121 RQLAKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNAVDQDGYVPVICGLSRCNENDIRTA 180
           RQLAKLGVDIIEAGFPAASKEDFEAVKIIAKEVGN VDQDGYVPVICGLSRCNE DIRTA
Sbjct: 121 RQLAKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKDIRTA 180

Query: 181 WEAVKYAKRPRIHTFIATSEIHMKHKLRKTREQVIEIARSMVRFARSLGCDDVEFSPEDA 240
           WEAVKYAKRPRIHTFIATSEIHMK+KLRKTRE+VIEIARSMVRFARSLGCDDVEFSPEDA
Sbjct: 181 WEAVKYAKRPRIHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFSPEDA 240

Query: 241 GRSDREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKSNTPGIENVIISTHC 300
           GRSDREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIK+NTPGIENVIISTHC
Sbjct: 241 GRSDREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVIISTHC 300

Query: 301 QNDLGLATANTVAGACSGARQVEVTINGIGERAGNASLEEASYLFNLIYFYYFIYSEIIM 360
           QNDLGLATANTVAGAC+GARQVEVTINGIGERAGNASLEE                    
Sbjct: 301 QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEE-------------------- 360

Query: 361 ILNYKFVVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAH 420
                 VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAH
Sbjct: 361 ------VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAH 420

Query: 421 ESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNEN 480
           ESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNEN
Sbjct: 421 ESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNEN 480

Query: 481 LDNIFWRFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLL 540
           LDNIFWRFK VAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLL
Sbjct: 481 LDNIFWRFKAVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLL 540

Query: 541 DADGKEHINCAVGTGPVDSAYKAVDGIVKETVTLLEYSMNAVTEGIDAIATTRVVIRGEN 600
           +ADGKEH NCAVGTGPVDSAYKAVDGIVKE V LLEYSMNAVTEGIDAIATTRVVIRGEN
Sbjct: 541 NADGKEHTNCAVGTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGEN 600

Query: 601 SYTSTNAMTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKV 649
           SYTSTNA+TGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKV
Sbjct: 601 SYTSTNAITGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKV 614

[Close]

BLAST of MS019122 vs. NCBI nr
Match: XP_038875433.1 (2-isopropylmalate synthase 2, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 1095.1 bits (2831), Expect = 0.0e+00
Identity = 563/657 (85.69%), Postives = 596/657 (90.72%), Query Frame = 0


Query: 1   MAIPTAFSRPN-HYFARQSNSQFPTCRQIYGRNLQFGNAVDSCPLSFRSAAGRVSRVYCC 60
           MA+P A SRPN H+ +RQSN+QFP   +IY  N Q      S  LSFR+AA  VSR+YCC
Sbjct: 1   MAMPAALSRPNTHFLSRQSNTQFPARHRIYSTNFQVLKN-PSRSLSFRTAADNVSRIYCC 60

Query: 61  QAQSIASDSKPNSSVRRPPYIPNRISDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQ 120
           Q +S+ S+S   SS+RRPPYIPNRI DPSYVRIFDTTLRDGEQSPGASLT+KEKLDIARQ
Sbjct: 61  QTESVVSNSGLKSSIRRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTAKEKLDIARQ 120

Query: 121 LAKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNAVDQDGYVPVICGLSRCNENDIRTAWE 180
           LAKLGVDIIEAGFPAASKEDFEAVK+IAKEVGNAVD+DGYVPVICGLSRCNENDIRTAWE
Sbjct: 121 LAKLGVDIIEAGFPAASKEDFEAVKMIAKEVGNAVDEDGYVPVICGLSRCNENDIRTAWE 180

Query: 181 AVKYAKRPRIHTFIATSEIHMKHKLRKTREQVIEIARSMVRFARSLGCDDVEFSPEDAGR 240
           AVKYAKRPRIHTFIATSEIHM+HKLRKTREQVIEIAR+MVRFARSLGCDDVEFSPEDAGR
Sbjct: 181 AVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDAGR 240

Query: 241 SDREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKSNTPGIENVIISTHCQN 300
           SDREFLY+ILGEVIKAGATTLNIPDTVGYTMPVEFG LIADIKSNTPGIENVIISTHCQN
Sbjct: 241 SDREFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGKLIADIKSNTPGIENVIISTHCQN 300

Query: 301 DLGLATANTVAGACSGARQVEVTINGIGERAGNASLEEASYLFNLIYFYYFIYSEIIMIL 360
           DLGLATANTVAGAC+GARQVEVTINGIGERAGNASLEE                      
Sbjct: 301 DLGLATANTVAGACAGARQVEVTINGIGERAGNASLEE---------------------- 360

Query: 361 NYKFVVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHES 420
               VVM+LQCRGEHVLGGLH+GINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHES
Sbjct: 361 ----VVMSLQCRGEHVLGGLHTGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHES 420

Query: 421 GIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLD 480
           GIHQDGMLKHKGTYEIISPEDIGYERSN+AGIVLGKLSGRHALKSRL ELGYELD+ENLD
Sbjct: 421 GIHQDGMLKHKGTYEIISPEDIGYERSNEAGIVLGKLSGRHALKSRLLELGYELDSENLD 480

Query: 481 NIFWRFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDA 540
           N+FWRFK VAEQKKRVTDADLRALVSDEVFQP+V+WKLLDLQVTCGTLGLSTATVKLLDA
Sbjct: 481 NVFWRFKAVAEQKKRVTDADLRALVSDEVFQPTVLWKLLDLQVTCGTLGLSTATVKLLDA 540

Query: 541 DGKEHINCAVGTGPVDSAYKAVDGIVKETVTLLEYSMNAVTEGIDAIATTRVVIRGENSY 600
           DGKEHI CAVGTGPVDSAYKAVD IVKE  TLLEYSMNAVTEGIDAIATTRV+IRG+ SY
Sbjct: 541 DGKEHIACAVGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSY 600

Query: 601 TSTNAMTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKVTEKRTLSA 657
           T+TNA+TGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGF GVDIKVT+++T+SA
Sbjct: 601 TATNALTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFHGVDIKVTQEKTVSA 630

[Close]

BLAST of MS019122 vs. NCBI nr
Match: XP_022984140.1 (2-isopropylmalate synthase 2, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1081.2 bits (2795), Expect = 0.0e+00
Identity = 557/656 (84.91%), Postives = 587/656 (89.48%), Query Frame = 0


Query: 1   MAIPTAFSRPNHYFARQSNSQFPTCRQIYGRNLQFGNAVDSCPLSFRSAAGRVSRVYCCQ 60
           MA+P AFSRPN +  RQ NSQF    +IY RNLQ      S  LSFR+AA  VSR+YCCQ
Sbjct: 1   MAMPAAFSRPNQFLVRQPNSQFSARHRIYSRNLQ-ALKNSSRSLSFRTAADHVSRIYCCQ 60

Query: 61  AQSIASDSKPNSSVRRPPYIPNRISDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQL 120
            +SI S+S+  SS+RRPPYIPNRI D SYVRIFDTTLRDGEQSPGASLT+KEK+DIARQL
Sbjct: 61  KESIVSNSESKSSIRRPPYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKVDIARQL 120

Query: 121 AKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNAVDQDGYVPVICGLSRCNENDIRTAWEA 180
            KLGVDIIEAGFPAASKEDFEAVK+IAKEVGNAVD+DGYVPVICGLSRCNE DIRTAWEA
Sbjct: 121 TKLGVDIIEAGFPAASKEDFEAVKMIAKEVGNAVDEDGYVPVICGLSRCNEKDIRTAWEA 180

Query: 181 VKYAKRPRIHTFIATSEIHMKHKLRKTREQVIEIARSMVRFARSLGCDDVEFSPEDAGRS 240
           VKY+KRPRIHTFIATSEIHM+HKLRKTREQVIEIAR+MV+FARSLGCDDVEFSPEDAGRS
Sbjct: 181 VKYSKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVQFARSLGCDDVEFSPEDAGRS 240

Query: 241 DREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKSNTPGIENVIISTHCQND 300
           DREFLY+ILGEVIKAGATTLNIPDTVGYTMP EFG LIADIKSNTPGIENVIISTHCQND
Sbjct: 241 DREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISTHCQND 300

Query: 301 LGLATANTVAGACSGARQVEVTINGIGERAGNASLEEASYLFNLIYFYYFIYSEIIMILN 360
           LGLATANTVAGAC+GARQVEVTINGIGERAGNASLEE                       
Sbjct: 301 LGLATANTVAGACAGARQVEVTINGIGERAGNASLEE----------------------- 360

Query: 361 YKFVVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESG 420
              VVMALQCRGEHVLGGL +GINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESG
Sbjct: 361 ---VVMALQCRGEHVLGGLRTGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESG 420

Query: 421 IHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDN 480
           IHQDGMLKHKGTYEII+PEDIGYERSNDAGIVLGKLSGRHALKSRL ELGYELDNE LDN
Sbjct: 421 IHQDGMLKHKGTYEIIAPEDIGYERSNDAGIVLGKLSGRHALKSRLVELGYELDNEKLDN 480

Query: 481 IFWRFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDAD 540
           +FWRFK VAEQKKRVTDAD+RALVSDEVFQP V+WKLLDLQVTCGTLGLSTATVKLLDAD
Sbjct: 481 VFWRFKAVAEQKKRVTDADIRALVSDEVFQPIVLWKLLDLQVTCGTLGLSTATVKLLDAD 540

Query: 541 GKEHINCAVGTGPVDSAYKAVDGIVKETVTLLEYSMNAVTEGIDAIATTRVVIRGENSYT 600
           G+EHI CAVGTGPVDSAYKAVD IVKE  TLLEYSM AVTEGIDAIATTRV+IRGE SYT
Sbjct: 541 GEEHIACAVGTGPVDSAYKAVDLIVKEPATLLEYSMTAVTEGIDAIATTRVLIRGEKSYT 600

Query: 601 STNAMTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKVTEKRTLSA 657
           +T+A TGE+VQRTFSGIGAGMDIVVSSVKAYIGALNKMLGF GVDIKVTEK+TLSA
Sbjct: 601 ATHASTGESVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFHGVDIKVTEKKTLSA 629

[Close]

BLAST of MS019122 vs. NCBI nr
Match: TYK01483.1 (2-isopropylmalate synthase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 555/659 (84.22%), Postives = 589/659 (89.38%), Query Frame = 0


Query: 1   MAIPTAFSRPNHYFARQSNSQFPTCRQIYGRNLQFGNAVDSCPLSFRSAAG---RVSRVY 60
           MA+P A SRPNH+ ARQS+S FP    IY RN Q      S  LSF++AA     VSR+Y
Sbjct: 1   MALPAALSRPNHFLARQSDSLFPARHPIYTRNFQVLKN-PSRSLSFKTAAAAPDNVSRIY 60

Query: 61  CCQAQSIASDSKPNSSVRRPPYIPNRISDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIA 120
           CC  +S+ S+S P SS RRPPYIPNRI DPSYVRIFDTTLRDGEQSPGASLT+KEKLDIA
Sbjct: 61  CCLTESVVSNSGPKSSTRRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTTKEKLDIA 120

Query: 121 RQLAKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNAVDQDGYVPVICGLSRCNENDIRTA 180
           RQLAKLGVDIIEAGFPAASKEDFEAVK+IAKE+G+AVD+DGYVPVICGLSRCNE DIRTA
Sbjct: 121 RQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGSAVDEDGYVPVICGLSRCNEKDIRTA 180

Query: 181 WEAVKYAKRPRIHTFIATSEIHMKHKLRKTREQVIEIARSMVRFARSLGCDDVEFSPEDA 240
           WEAVKYAKRPRIHTFIATSEIHM+HKLRKTREQVIEIAR+MVRFARSLGCDDVEFSPEDA
Sbjct: 181 WEAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDA 240

Query: 241 GRSDREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKSNTPGIENVIISTHC 300
           GRSDREFLY+ILGEVIKAGATTLNIPDTVGYTMP EFG LIADIKSNTPGIENVIISTHC
Sbjct: 241 GRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISTHC 300

Query: 301 QNDLGLATANTVAGACSGARQVEVTINGIGERAGNASLEEASYLFNLIYFYYFIYSEIIM 360
           QNDLGLATANTVAGAC+GARQVEVTINGIGERAGNASLEE                    
Sbjct: 301 QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEE-------------------- 360

Query: 361 ILNYKFVVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAH 420
                 VVMALQCRGEHVLGGLH+GIN RHIFLTSKMVEE+TGLNVQPHKAIVGANAFAH
Sbjct: 361 ------VVMALQCRGEHVLGGLHTGINPRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAH 420

Query: 421 ESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNEN 480
            SGIHQDGMLKHKGTYEI++PEDIGYERSNDAGIVLGKLSGRHALKSRL ELGYELD+EN
Sbjct: 421 ASGIHQDGMLKHKGTYEIMAPEDIGYERSNDAGIVLGKLSGRHALKSRLLELGYELDSEN 480

Query: 481 LDNIFWRFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLL 540
           L N+FWRFK VAEQKKRVTDADLRALVSDEVFQP+V+WKLLDLQVTCGTLGLSTATVKLL
Sbjct: 481 LHNVFWRFKAVAEQKKRVTDADLRALVSDEVFQPTVLWKLLDLQVTCGTLGLSTATVKLL 540

Query: 541 DADGKEHINCAVGTGPVDSAYKAVDGIVKETVTLLEYSMNAVTEGIDAIATTRVVIRGEN 600
           DADGKEHI C+VGTGPVDSAYKAVD IVKE  TLLEYSMNAVTEGIDAIATTRV+IRG+ 
Sbjct: 541 DADGKEHIACSVGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDK 600

Query: 601 SYTSTNAMTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKVTEKRTLSA 657
           SYTSTNA+TGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQG+DIK T+++TLSA
Sbjct: 601 SYTSTNALTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGIDIKETKEKTLSA 632

[Close]

BLAST of MS019122 vs. ExPASy Swiss-Prot
Match: Q9C550 (2-isopropylmalate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPMS2 PE=1 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 1.9e-265
Identity = 462/580 (79.66%), Postives = 517/580 (89.14%), Query Frame = 0


Query: 63  SIASDSKPNSSVRRPPYIPNRISDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQLAK 122
           S +S   P++  RRP YIPNRISDP+YVRIFDTTLRDGEQSPGA+LTSKEKLDIARQLAK
Sbjct: 60  SASSPLPPHAPRRRPNYIPNRISDPNYVRIFDTTLRDGEQSPGATLTSKEKLDIARQLAK 119

Query: 123 LGVDIIEAGFPAASKEDFEAVKIIAKEVGNAVDQDGYVPVICGLSRCNENDIRTAWEAVK 182
           LGVDIIEAGFPAASK+DFEAVK IA+ VGN VD++GYVPVICGLSRCN+ DI TAWEAVK
Sbjct: 120 LGVDIIEAGFPAASKDDFEAVKTIAETVGNTVDENGYVPVICGLSRCNKKDIETAWEAVK 179

Query: 183 YAKRPRIHTFIATSEIHMKHKLRKTREQVIEIARSMVRFARSLGCDDVEFSPEDAGRSDR 242
           YAKRPRIHTFIATS+IH+K+KL+K++E+VIEIAR+MVRFARSLGC+DVEFSPEDAGRS+R
Sbjct: 180 YAKRPRIHTFIATSDIHLKYKLKKSKEEVIEIARNMVRFARSLGCEDVEFSPEDAGRSER 239

Query: 243 EFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKSNTPGIENVIISTHCQNDLG 302
           E+LYEILGEVIKAGATTLNIPDTVG T+P EFG LIADIK+NTPGI+NVIISTHCQNDLG
Sbjct: 240 EYLYEILGEVIKAGATTLNIPDTVGITLPSEFGQLIADIKANTPGIQNVIISTHCQNDLG 299

Query: 303 LATANTVAGACSGARQVEVTINGIGERAGNASLEEASYLFNLIYFYYFIYSEIIMILNYK 362
           L+TANT++GA SGARQVEVTINGIGERAGNASLEE                         
Sbjct: 300 LSTANTLSGAHSGARQVEVTINGIGERAGNASLEE------------------------- 359

Query: 363 FVVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIH 422
            VVMA++CRG+HVLGGL +GI++RHI +TSKMVEEYTG+  QPHKAIVGANAFAHESGIH
Sbjct: 360 -VVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGMQTQPHKAIVGANAFAHESGIH 419

Query: 423 QDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIF 482
           QDGMLKHKGTYEI+SPE+IG ERSNDAGIVLGKLSGRHALK RL ELGY LD+  L N+F
Sbjct: 420 QDGMLKHKGTYEIMSPEEIGLERSNDAGIVLGKLSGRHALKDRLNELGYVLDDGQLSNLF 479

Query: 483 WRFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDADGK 542
           WRFK VAEQKKRVTDADL ALVSDEVFQP  VWKLLD+Q+TCGTLGLST+TVKL D+DGK
Sbjct: 480 WRFKAVAEQKKRVTDADLIALVSDEVFQPEAVWKLLDMQITCGTLGLSTSTVKLADSDGK 539

Query: 543 EHINCAVGTGPVDSAYKAVDGIVKETVTLLEYSMNAVTEGIDAIATTRVVIRGENSYTST 602
           EH+ C+VGTGPVD+AYKAVD IVKE  TLLEYSMNAVTEGIDAIATTRV+IRG+N+Y+ST
Sbjct: 540 EHVACSVGTGPVDAAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDNNYSST 599

Query: 603 NAMTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQ 643
           NA+TGE+V+RTFSG GAGMDIVVSSVKAY+GALNKMLGF+
Sbjct: 600 NAVTGESVERTFSGTGAGMDIVVSSVKAYVGALNKMLGFK 613

[Close]

BLAST of MS019122 vs. ExPASy Swiss-Prot
Match: Q9LPR4 (2-isopropylmalate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPMS1 PE=1 SV=2)

HSP 1 Score: 902.9 bits (2332), Expect = 2.1e-261
Identity = 452/567 (79.72%), Postives = 503/567 (88.71%), Query Frame = 0


Query: 76  RPPYIPNRISDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQLAKLGVDIIEAGFPAA 135
           RP YIPNRISDP+YVR+FDTTLRDGEQSPGA+LTSKEKLDIARQLAKLGVDIIEAGFPAA
Sbjct: 75  RPEYIPNRISDPNYVRVFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAA 134

Query: 136 SKEDFEAVKIIAKEVGNAVDQDGYVPVICGLSRCNENDIRTAWEAVKYAKRPRIHTFIAT 195
           SK+DFEAVK IA+ VGN VD++GYVPVICGLSRCN+ DI  AW+AVKYAKRPRIHTFIAT
Sbjct: 135 SKDDFEAVKTIAETVGNTVDENGYVPVICGLSRCNKKDIERAWDAVKYAKRPRIHTFIAT 194

Query: 196 SEIHMKHKLRKTREQVIEIARSMVRFARSLGCDDVEFSPEDAGRSDREFLYEILGEVIKA 255
           S+IH+++KL+KT+ +VIEIARSMVRFARSLGC+DVEFSPEDAGRS+RE+LYEILGEVIKA
Sbjct: 195 SDIHLEYKLKKTKAEVIEIARSMVRFARSLGCEDVEFSPEDAGRSEREYLYEILGEVIKA 254

Query: 256 GATTLNIPDTVGYTMPVEFGALIADIKSNTPGIENVIISTHCQNDLGLATANTVAGACSG 315
           GATTLNIPDTVG T+P EFG LI D+K+NTPGIENV+ISTHCQNDLGL+TANT++GA +G
Sbjct: 255 GATTLNIPDTVGITLPSEFGQLITDLKANTPGIENVVISTHCQNDLGLSTANTLSGAHAG 314

Query: 316 ARQVEVTINGIGERAGNASLEEASYLFNLIYFYYFIYSEIIMILNYKFVVMALQCRGEHV 375
           ARQ+EVTINGIGERAGNASLEE                          VVMA++CRG+HV
Sbjct: 315 ARQMEVTINGIGERAGNASLEE--------------------------VVMAIKCRGDHV 374

Query: 376 LGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEI 435
           LGGL +GI++RHI +TSKMVEEYTG+  QPHKAIVGANAFAHESGIHQDGMLKHKGTYEI
Sbjct: 375 LGGLFTGIDTRHIVMTSKMVEEYTGMQTQPHKAIVGANAFAHESGIHQDGMLKHKGTYEI 434

Query: 436 ISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRFKEVAEQKKRV 495
           I PE+IG ERSNDAGIVLGKLSGRHALK RL ELGY+LD+E L  IFWRFK VAEQKKRV
Sbjct: 435 ICPEEIGLERSNDAGIVLGKLSGRHALKDRLTELGYQLDDEQLSTIFWRFKTVAEQKKRV 494

Query: 496 TDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDADGKEHINCAVGTGPVD 555
           TDAD+ ALVSDEVFQP  VWKLLD+Q+TCGTLGLSTATVKL DADGKEH+ C++GTGPVD
Sbjct: 495 TDADIIALVSDEVFQPEAVWKLLDIQITCGTLGLSTATVKLADADGKEHVACSIGTGPVD 554

Query: 556 SAYKAVDGIVKETVTLLEYSMNAVTEGIDAIATTRVVIRGENSYTSTNAMTGEAVQRTFS 615
           SAYKAVD IVKE  TLLEYSMNAVTEGIDAIATTRV+IRG N Y+STNA+TGE VQRTFS
Sbjct: 555 SAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGSNKYSSTNAITGEEVQRTFS 614

Query: 616 GIGAGMDIVVSSVKAYIGALNKMLGFQ 643
           G GAGMDIVVSSVKAY+GALNKM+ F+
Sbjct: 615 GTGAGMDIVVSSVKAYVGALNKMMDFK 615

[Close]

BLAST of MS019122 vs. ExPASy Swiss-Prot
Match: O04973 (2-isopropylmalate synthase A OS=Solanum pennellii OX=28526 GN=IPMSA PE=2 SV=1)

HSP 1 Score: 845.9 bits (2184), Expect = 3.1e-244
Identity = 419/570 (73.51%), Postives = 494/570 (86.67%), Query Frame = 0


Query: 73  SVRRPPYIPNRISDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQLAKLGVDIIEAGF 132
           S RRP Y+P++ISDP YVRIFDTTLRDGEQSPGA++T+KEKLD+ARQLAKLGVDIIEAGF
Sbjct: 31  SNRRPEYVPSKISDPKYVRIFDTTLRDGEQSPGATMTTKEKLDVARQLAKLGVDIIEAGF 90

Query: 133 PAASKEDFEAVKIIAKEVGNAVDQDGYVPVICGLSRCNENDIRTAWEAVKYAKRPRIHTF 192
           PA+S+ DFE+VK+IA+E+GN  D++G+VPVICGLSRCN++DI  AWEAVKYAK+PR+HTF
Sbjct: 91  PASSEADFESVKLIAEEIGNNTDENGFVPVICGLSRCNKSDIDKAWEAVKYAKKPRVHTF 150

Query: 193 IATSEIHMKHKLRKTREQVIEIARSMVRFARSLGCDDVEFSPEDAGRSDREFLYEILGEV 252
           IATSEIHMK+KL+ +REQV+E ARSMV +ARSLGC+DVEFSPEDAGRSDREFLY+ILGEV
Sbjct: 151 IATSEIHMKYKLKMSREQVVEKARSMVAYARSLGCEDVEFSPEDAGRSDREFLYDILGEV 210

Query: 253 IKAGATTLNIPDTVGYTMPVEFGALIADIKSNTPGIENVIISTHCQNDLGLATANTVAGA 312
           IKAGATTLNIPDTVGYT+P EFG LI DIK+NTPGIENVIISTHCQNDLGL+TANT+AGA
Sbjct: 211 IKAGATTLNIPDTVGYTVPSEFGQLITDIKANTPGIENVIISTHCQNDLGLSTANTLAGA 270

Query: 313 CSGARQVEVTINGIGERAGNASLEEASYLFNLIYFYYFIYSEIIMILNYKFVVMALQCRG 372
           C+GARQ+EVTINGIGERAGNASLEE                          VVMAL+CRG
Sbjct: 271 CAGARQLEVTINGIGERAGNASLEE--------------------------VVMALKCRG 330

Query: 373 EHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGT 432
           E VLGGL++GIN++HI  +SKMVEEY+GL VQPHKAIVGANAFAHESGIHQDGMLKHK T
Sbjct: 331 EQVLGGLYTGINTQHIVPSSKMVEEYSGLQVQPHKAIVGANAFAHESGIHQDGMLKHKDT 390

Query: 433 YEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRFKEVAEQK 492
           YEIISP+D+G  RSN+AGIVLGKLSGRHALKS++ ELGY++D + L+++FWRFK VAE+K
Sbjct: 391 YEIISPDDVGLSRSNEAGIVLGKLSGRHALKSKMLELGYDIDGKELEDLFWRFKSVAEKK 450

Query: 493 KRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDADGKEHINCAVGTG 552
           K++TD DL AL+SDEV QP+V WKL D+Q+ CG+LGLSTATVKL++ DG+EHI C+VGTG
Sbjct: 451 KKITDDDLIALMSDEVLQPNVYWKLGDVQIMCGSLGLSTATVKLINTDGQEHIACSVGTG 510

Query: 553 PVDSAYKAVDGIVKETVTLLEYSMNAVTEGIDAIATTRVVIRGENSYTSTNAMTGEAVQR 612
           PVD+AYKAVD IVK  +TLLEYSMNAVTEGIDAIA+TRV I   + +T  N  TG+ + R
Sbjct: 511 PVDAAYKAVDLIVKVPITLLEYSMNAVTEGIDAIASTRVSICSIDRHTIMNGSTGQTIHR 570

Query: 613 TFSGIGAGMDIVVSSVKAYIGALNKMLGFQ 643
           TFSG GA MD+V+SSV+AYIGALNKML ++
Sbjct: 571 TFSGTGADMDVVISSVRAYIGALNKMLSYE 574

[Close]

BLAST of MS019122 vs. ExPASy Swiss-Prot
Match: O04974 (2-isopropylmalate synthase B OS=Solanum pennellii OX=28526 GN=IPMSB PE=2 SV=1)

HSP 1 Score: 830.1 bits (2143), Expect = 1.8e-239
Identity = 415/571 (72.68%), Postives = 490/571 (85.81%), Query Frame = 0


Query: 73  SVRR-PPYIPNRISDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQLAKLGVDIIEAG 132
           S+RR P Y P+ I DP+YVRIFDTTLRDGEQSPGA++T+KEKLD+ARQ AKLGVDIIEAG
Sbjct: 53  SIRRIPEYTPSHIPDPNYVRIFDTTLRDGEQSPGATMTTKEKLDVARQSAKLGVDIIEAG 112

Query: 133 FPAASKEDFEAVKIIAKEVGNAVDQDGYVPVICGLSRCNENDIRTAWEAVKYAKRPRIHT 192
           FPA+S+ D EAVK+IAKEVGN V ++ YVPVICGL+RCN+ DI  AWEAVKYAK+PRIHT
Sbjct: 113 FPASSEADLEAVKLIAKEVGNGVYEEEYVPVICGLARCNKKDIDKAWEAVKYAKKPRIHT 172

Query: 193 FIATSEIHMKHKLRKTREQVIEIARSMVRFARSLGCDDVEFSPEDAGRSDREFLYEILGE 252
           FIATSE+HM +KL+ +R+QV+E ARSMV +ARS+GC+DVEFSPEDAGRSD EFLY ILGE
Sbjct: 173 FIATSEVHMNYKLKMSRDQVVEKARSMVAYARSIGCEDVEFSPEDAGRSDPEFLYHILGE 232

Query: 253 VIKAGATTLNIPDTVGYTMPVEFGALIADIKSNTPGIENVIISTHCQNDLGLATANTVAG 312
           VIKAGATTLNIPDTVGYT+P EFG LIA IK+NTPG+E+VIISTHCQNDLGL+TANT+AG
Sbjct: 233 VIKAGATTLNIPDTVGYTVPEEFGQLIAKIKANTPGVEDVIISTHCQNDLGLSTANTLAG 292

Query: 313 ACSGARQVEVTINGIGERAGNASLEEASYLFNLIYFYYFIYSEIIMILNYKFVVMALQCR 372
           AC+GARQ+EVTINGIGERAGNASLEE                          VVMAL+CR
Sbjct: 293 ACAGARQLEVTINGIGERAGNASLEE--------------------------VVMALKCR 352

Query: 373 GEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKG 432
           GE VLGGL++GIN++HI ++SKMVE  +GL+VQPHKAIVGANAF HESGIHQDGMLKHK 
Sbjct: 353 GEQVLGGLYTGINTQHILMSSKMVEGISGLHVQPHKAIVGANAFVHESGIHQDGMLKHKD 412

Query: 433 TYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRFKEVAEQ 492
           TYEIISPEDIG  R+N++GIV GKLSG    K ++ ELGYE++ + LD++FWRFK VAE+
Sbjct: 413 TYEIISPEDIGLNRANESGIVFGKLSGVMLCKPKMLELGYEIEGKELDDLFWRFKSVAEK 472

Query: 493 KKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDADGKEHINCAVGT 552
           KK++TD DL AL+SDEVFQP  VW+L ++QVTCG+LGLSTATVKL+DADG+EHI+C+VGT
Sbjct: 473 KKKITDDDLVALMSDEVFQPQFVWQLQNVQVTCGSLGLSTATVKLIDADGREHISCSVGT 532

Query: 553 GPVDSAYKAVDGIVKETVTLLEYSMNAVTEGIDAIATTRVVIRGENSYTSTNAMTGEAVQ 612
           GPVD+AYKAVD IVK  VTLLEYSMNAVT+GIDAIA+TRV+IRGEN +TST+A+TGE V 
Sbjct: 533 GPVDAAYKAVDLIVKVPVTLLEYSMNAVTQGIDAIASTRVLIRGENGHTSTHALTGETVH 592

Query: 613 RTFSGIGAGMDIVVSSVKAYIGALNKMLGFQ 643
           RTFSG GA MDIV+SSV+AY+GALNKM+ F+
Sbjct: 593 RTFSGTGADMDIVISSVRAYVGALNKMMSFR 597

[Close]

BLAST of MS019122 vs. ExPASy Swiss-Prot
Match: Q39891 (Probable 2-isopropylmalate synthase OS=Glycine max OX=3847 GN=GMN56 PE=2 SV=1)

HSP 1 Score: 740.0 bits (1909), Expect = 2.4e-212
Identity = 381/577 (66.03%), Postives = 449/577 (77.82%), Query Frame = 0


Query: 63  SIASDSKPNSSVRRPPYIPNRISDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQLAK 122
           S  S   P  S  RP YIPN I D SYVRI DTTLRDGEQSPGA++T+KEKLDIARQL K
Sbjct: 6   STPSSQSPKLSHLRPQYIPNHIPDSSYVRILDTTLRDGEQSPGATMTAKEKLDIARQLVK 65

Query: 123 LGVDIIEAGFPAASKEDFEAVKIIAKEVGNAVDQDGYVPVICGLSRCNENDIRTAWEAVK 182
           LGVDII+ GFP+AS  DF AVK+IA+EVGNAVD DGYVPVI G  RC E DI TAWEAVK
Sbjct: 66  LGVDIIQPGFPSASNSDFMAVKMIAQEVGNAVDDDGYVPVIAGFCRCVEKDISTAWEAVK 125

Query: 183 YAKRPRIHTFIATSEIHMKHKLRKTREQVIEIARSMVRFARSLGCDDVEFSPEDAGRSDR 242
           YAKRPR+ T IATS IHM+HKLRK+++QVI+IAR MV+FARSLGC+D++F  EDA RSDR
Sbjct: 126 YAKRPRLCTSIATSPIHMEHKLRKSKDQVIQIARDMVKFARSLGCNDIQFGAEDATRSDR 185

Query: 243 EFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKSNTPGIENVIISTHCQNDLG 302
           EFLYEILG VI+AGATT+NI DTVG  MP+E G LI DIK NTPGI NVIISTHC NDLG
Sbjct: 186 EFLYEILGVVIEAGATTVNIADTVGIVMPLELGKLIVDIKDNTPGIANVIISTHCHNDLG 245

Query: 303 LATANTVAGACSGARQVEVTINGIGERAGNASLEEASYLFNLIYFYYFIYSEIIMILNYK 362
           LATANT+ GA +GARQ+EVTINGIGERAGNASLEE                         
Sbjct: 246 LATANTIEGARTGARQLEVTINGIGERAGNASLEE------------------------- 305

Query: 363 FVVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIH 422
            VVMAL  +G+H L GL++ IN+RHI  TSKMVEEY+G+++QPHK +VGANAF H SGIH
Sbjct: 306 -VVMALASKGDHALNGLYTRINTRHILETSKMVEEYSGMHLQPHKPLVGANAFVHASGIH 365

Query: 423 QDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIF 482
           QDGMLKHKGTYE ISPE+IG++R+   GIVLGKLSG  AL+ RL+ELGY+L  + +D++F
Sbjct: 366 QDGMLKHKGTYETISPEEIGHKRTTRIGIVLGKLSGSQALRKRLEELGYDLKEDEVDSVF 425

Query: 483 WRFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDADGK 542
           W+FK +AE+KK VTD DL+ALVS + F    +WKL DLQVTCGT+GLSTATVKL++ DG 
Sbjct: 426 WQFKAMAEKKKVVTDVDLKALVSYKAFHAESIWKLGDLQVTCGTIGLSTATVKLVNIDGS 485

Query: 543 EHINCAVGTGPVDSAYKAVDGIVKETVTLLEYSMNAVTEGIDAIATTRVVIRGENSYTST 602
            H+ C++G G VDS YKA++ IVKE   LL+YS+N+VTEGI    T RVVI  EN++TST
Sbjct: 486 THVACSIGIGAVDSTYKAINLIVKEPTKLLDYSLNSVTEGIGVNVTARVVICRENNHTST 545

Query: 603 NAMTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKML 640
            A T +A   TFSGI A MD+VVS+VKAY+ ALNK+L
Sbjct: 546 YAFTEDANYPTFSGIAAEMDVVVSTVKAYLVALNKLL 556

[Close]

BLAST of MS019122 vs. ExPASy TrEMBL
Match: A0A6J1CKJ3 (2-isopropylmalate synthase OS=Momordica charantia OX=3673 GN=LOC111012138 PE=3 SV=1)

HSP 1 Score: 1123.6 bits (2905), Expect = 0.0e+00
Identity = 590/659 (89.53%), Postives = 599/659 (90.90%), Query Frame = 0


Query: 1   MAIPTAFSRPNHYFARQSNSQFPTCRQIYGRNLQFGNAVDSCPLSFRSAAGRVSRVYCC- 60
           MA+ TAFS PNH F RQ NSQFPT   IY RNLQ        P SFRS AGRV    CC 
Sbjct: 1   MAMATAFSSPNHCFGRQWNSQFPTSGHIYRRNLQ-------NPSSFRSPAGRV----CCS 60

Query: 61  --QAQSIASDSKPNSSVRRPPYIPNRISDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIA 120
             Q+ SIASDSKPNSSV+RPPYIPNRI DPSYVRIFDTTLRDGEQSPGASLTSKEKLDIA
Sbjct: 61  EAQSISIASDSKPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIA 120

Query: 121 RQLAKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNAVDQDGYVPVICGLSRCNENDIRTA 180
           RQLAKLGVDIIEAGFPAASKEDFEAVKIIAKEVGN VDQDGYVPVICGLSRCNE DIRTA
Sbjct: 121 RQLAKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKDIRTA 180

Query: 181 WEAVKYAKRPRIHTFIATSEIHMKHKLRKTREQVIEIARSMVRFARSLGCDDVEFSPEDA 240
           WEAVKYAKRPRIHTFIATSEIHMK+KLRKTRE+VIEIARSMVRFARSLGCDDVEFSPEDA
Sbjct: 181 WEAVKYAKRPRIHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFSPEDA 240

Query: 241 GRSDREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKSNTPGIENVIISTHC 300
           GRSDREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIK+NTPGIENVIISTHC
Sbjct: 241 GRSDREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVIISTHC 300

Query: 301 QNDLGLATANTVAGACSGARQVEVTINGIGERAGNASLEEASYLFNLIYFYYFIYSEIIM 360
           QNDLGLATANTVAGAC+GARQVEVTINGIGERAGNASLEE                    
Sbjct: 301 QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEE-------------------- 360

Query: 361 ILNYKFVVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAH 420
                 VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAH
Sbjct: 361 ------VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAH 420

Query: 421 ESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNEN 480
           ESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNEN
Sbjct: 421 ESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNEN 480

Query: 481 LDNIFWRFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLL 540
           LDNIFWRFK VAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLL
Sbjct: 481 LDNIFWRFKAVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLL 540

Query: 541 DADGKEHINCAVGTGPVDSAYKAVDGIVKETVTLLEYSMNAVTEGIDAIATTRVVIRGEN 600
           +ADGKEH NCAVGTGPVDSAYKAVDGIVKE V LLEYSMNAVTEGIDAIATTRVVIRGEN
Sbjct: 541 NADGKEHTNCAVGTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGEN 600

Query: 601 SYTSTNAMTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKVTEKRTLSA 657
           SYTSTNA+TGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKVTEKRTLSA
Sbjct: 601 SYTSTNAITGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKVTEKRTLSA 622

[Close]

BLAST of MS019122 vs. ExPASy TrEMBL
Match: A0A6J1CL37 (2-isopropylmalate synthase OS=Momordica charantia OX=3673 GN=LOC111012138 PE=3 SV=1)

HSP 1 Score: 1108.6 bits (2866), Expect = 0.0e+00
Identity = 582/651 (89.40%), Postives = 591/651 (90.78%), Query Frame = 0


Query: 1   MAIPTAFSRPNHYFARQSNSQFPTCRQIYGRNLQFGNAVDSCPLSFRSAAGRVSRVYCC- 60
           MA+ TAFS PNH F RQ NSQFPT   IY RNLQ        P SFRS AGRV    CC 
Sbjct: 1   MAMATAFSSPNHCFGRQWNSQFPTSGHIYRRNLQ-------NPSSFRSPAGRV----CCS 60

Query: 61  --QAQSIASDSKPNSSVRRPPYIPNRISDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIA 120
             Q+ SIASDSKPNSSV+RPPYIPNRI DPSYVRIFDTTLRDGEQSPGASLTSKEKLDIA
Sbjct: 61  EAQSISIASDSKPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIA 120

Query: 121 RQLAKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNAVDQDGYVPVICGLSRCNENDIRTA 180
           RQLAKLGVDIIEAGFPAASKEDFEAVKIIAKEVGN VDQDGYVPVICGLSRCNE DIRTA
Sbjct: 121 RQLAKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKDIRTA 180

Query: 181 WEAVKYAKRPRIHTFIATSEIHMKHKLRKTREQVIEIARSMVRFARSLGCDDVEFSPEDA 240
           WEAVKYAKRPRIHTFIATSEIHMK+KLRKTRE+VIEIARSMVRFARSLGCDDVEFSPEDA
Sbjct: 181 WEAVKYAKRPRIHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFSPEDA 240

Query: 241 GRSDREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKSNTPGIENVIISTHC 300
           GRSDREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIK+NTPGIENVIISTHC
Sbjct: 241 GRSDREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVIISTHC 300

Query: 301 QNDLGLATANTVAGACSGARQVEVTINGIGERAGNASLEEASYLFNLIYFYYFIYSEIIM 360
           QNDLGLATANTVAGAC+GARQVEVTINGIGERAGNASLEE                    
Sbjct: 301 QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEE-------------------- 360

Query: 361 ILNYKFVVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAH 420
                 VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAH
Sbjct: 361 ------VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAH 420

Query: 421 ESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNEN 480
           ESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNEN
Sbjct: 421 ESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNEN 480

Query: 481 LDNIFWRFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLL 540
           LDNIFWRFK VAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLL
Sbjct: 481 LDNIFWRFKAVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLL 540

Query: 541 DADGKEHINCAVGTGPVDSAYKAVDGIVKETVTLLEYSMNAVTEGIDAIATTRVVIRGEN 600
           +ADGKEH NCAVGTGPVDSAYKAVDGIVKE V LLEYSMNAVTEGIDAIATTRVVIRGEN
Sbjct: 541 NADGKEHTNCAVGTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGEN 600

Query: 601 SYTSTNAMTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKV 649
           SYTSTNA+TGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKV
Sbjct: 601 SYTSTNAITGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKV 614

[Close]

BLAST of MS019122 vs. ExPASy TrEMBL
Match: A0A6J1J7V5 (2-isopropylmalate synthase OS=Cucurbita maxima OX=3661 GN=LOC111482524 PE=3 SV=1)

HSP 1 Score: 1081.2 bits (2795), Expect = 0.0e+00
Identity = 557/656 (84.91%), Postives = 587/656 (89.48%), Query Frame = 0


Query: 1   MAIPTAFSRPNHYFARQSNSQFPTCRQIYGRNLQFGNAVDSCPLSFRSAAGRVSRVYCCQ 60
           MA+P AFSRPN +  RQ NSQF    +IY RNLQ      S  LSFR+AA  VSR+YCCQ
Sbjct: 1   MAMPAAFSRPNQFLVRQPNSQFSARHRIYSRNLQ-ALKNSSRSLSFRTAADHVSRIYCCQ 60

Query: 61  AQSIASDSKPNSSVRRPPYIPNRISDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQL 120
            +SI S+S+  SS+RRPPYIPNRI D SYVRIFDTTLRDGEQSPGASLT+KEK+DIARQL
Sbjct: 61  KESIVSNSESKSSIRRPPYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKVDIARQL 120

Query: 121 AKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNAVDQDGYVPVICGLSRCNENDIRTAWEA 180
            KLGVDIIEAGFPAASKEDFEAVK+IAKEVGNAVD+DGYVPVICGLSRCNE DIRTAWEA
Sbjct: 121 TKLGVDIIEAGFPAASKEDFEAVKMIAKEVGNAVDEDGYVPVICGLSRCNEKDIRTAWEA 180

Query: 181 VKYAKRPRIHTFIATSEIHMKHKLRKTREQVIEIARSMVRFARSLGCDDVEFSPEDAGRS 240
           VKY+KRPRIHTFIATSEIHM+HKLRKTREQVIEIAR+MV+FARSLGCDDVEFSPEDAGRS
Sbjct: 181 VKYSKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVQFARSLGCDDVEFSPEDAGRS 240

Query: 241 DREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKSNTPGIENVIISTHCQND 300
           DREFLY+ILGEVIKAGATTLNIPDTVGYTMP EFG LIADIKSNTPGIENVIISTHCQND
Sbjct: 241 DREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISTHCQND 300

Query: 301 LGLATANTVAGACSGARQVEVTINGIGERAGNASLEEASYLFNLIYFYYFIYSEIIMILN 360
           LGLATANTVAGAC+GARQVEVTINGIGERAGNASLEE                       
Sbjct: 301 LGLATANTVAGACAGARQVEVTINGIGERAGNASLEE----------------------- 360

Query: 361 YKFVVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESG 420
              VVMALQCRGEHVLGGL +GINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESG
Sbjct: 361 ---VVMALQCRGEHVLGGLRTGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESG 420

Query: 421 IHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDN 480
           IHQDGMLKHKGTYEII+PEDIGYERSNDAGIVLGKLSGRHALKSRL ELGYELDNE LDN
Sbjct: 421 IHQDGMLKHKGTYEIIAPEDIGYERSNDAGIVLGKLSGRHALKSRLVELGYELDNEKLDN 480

Query: 481 IFWRFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDAD 540
           +FWRFK VAEQKKRVTDAD+RALVSDEVFQP V+WKLLDLQVTCGTLGLSTATVKLLDAD
Sbjct: 481 VFWRFKAVAEQKKRVTDADIRALVSDEVFQPIVLWKLLDLQVTCGTLGLSTATVKLLDAD 540

Query: 541 GKEHINCAVGTGPVDSAYKAVDGIVKETVTLLEYSMNAVTEGIDAIATTRVVIRGENSYT 600
           G+EHI CAVGTGPVDSAYKAVD IVKE  TLLEYSM AVTEGIDAIATTRV+IRGE SYT
Sbjct: 541 GEEHIACAVGTGPVDSAYKAVDLIVKEPATLLEYSMTAVTEGIDAIATTRVLIRGEKSYT 600

Query: 601 STNAMTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKVTEKRTLSA 657
           +T+A TGE+VQRTFSGIGAGMDIVVSSVKAYIGALNKMLGF GVDIKVTEK+TLSA
Sbjct: 601 ATHASTGESVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFHGVDIKVTEKKTLSA 629

[Close]

BLAST of MS019122 vs. ExPASy TrEMBL
Match: A0A5D3BP53 (2-isopropylmalate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G00350 PE=3 SV=1)

HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 555/659 (84.22%), Postives = 589/659 (89.38%), Query Frame = 0


Query: 1   MAIPTAFSRPNHYFARQSNSQFPTCRQIYGRNLQFGNAVDSCPLSFRSAAG---RVSRVY 60
           MA+P A SRPNH+ ARQS+S FP    IY RN Q      S  LSF++AA     VSR+Y
Sbjct: 1   MALPAALSRPNHFLARQSDSLFPARHPIYTRNFQVLKN-PSRSLSFKTAAAAPDNVSRIY 60

Query: 61  CCQAQSIASDSKPNSSVRRPPYIPNRISDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIA 120
           CC  +S+ S+S P SS RRPPYIPNRI DPSYVRIFDTTLRDGEQSPGASLT+KEKLDIA
Sbjct: 61  CCLTESVVSNSGPKSSTRRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTTKEKLDIA 120

Query: 121 RQLAKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNAVDQDGYVPVICGLSRCNENDIRTA 180
           RQLAKLGVDIIEAGFPAASKEDFEAVK+IAKE+G+AVD+DGYVPVICGLSRCNE DIRTA
Sbjct: 121 RQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGSAVDEDGYVPVICGLSRCNEKDIRTA 180

Query: 181 WEAVKYAKRPRIHTFIATSEIHMKHKLRKTREQVIEIARSMVRFARSLGCDDVEFSPEDA 240
           WEAVKYAKRPRIHTFIATSEIHM+HKLRKTREQVIEIAR+MVRFARSLGCDDVEFSPEDA
Sbjct: 181 WEAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDA 240

Query: 241 GRSDREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKSNTPGIENVIISTHC 300
           GRSDREFLY+ILGEVIKAGATTLNIPDTVGYTMP EFG LIADIKSNTPGIENVIISTHC
Sbjct: 241 GRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISTHC 300

Query: 301 QNDLGLATANTVAGACSGARQVEVTINGIGERAGNASLEEASYLFNLIYFYYFIYSEIIM 360
           QNDLGLATANTVAGAC+GARQVEVTINGIGERAGNASLEE                    
Sbjct: 301 QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEE-------------------- 360

Query: 361 ILNYKFVVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAH 420
                 VVMALQCRGEHVLGGLH+GIN RHIFLTSKMVEE+TGLNVQPHKAIVGANAFAH
Sbjct: 361 ------VVMALQCRGEHVLGGLHTGINPRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAH 420

Query: 421 ESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNEN 480
            SGIHQDGMLKHKGTYEI++PEDIGYERSNDAGIVLGKLSGRHALKSRL ELGYELD+EN
Sbjct: 421 ASGIHQDGMLKHKGTYEIMAPEDIGYERSNDAGIVLGKLSGRHALKSRLLELGYELDSEN 480

Query: 481 LDNIFWRFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLL 540
           L N+FWRFK VAEQKKRVTDADLRALVSDEVFQP+V+WKLLDLQVTCGTLGLSTATVKLL
Sbjct: 481 LHNVFWRFKAVAEQKKRVTDADLRALVSDEVFQPTVLWKLLDLQVTCGTLGLSTATVKLL 540

Query: 541 DADGKEHINCAVGTGPVDSAYKAVDGIVKETVTLLEYSMNAVTEGIDAIATTRVVIRGEN 600
           DADGKEHI C+VGTGPVDSAYKAVD IVKE  TLLEYSMNAVTEGIDAIATTRV+IRG+ 
Sbjct: 541 DADGKEHIACSVGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDK 600

Query: 601 SYTSTNAMTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKVTEKRTLSA 657
           SYTSTNA+TGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQG+DIK T+++TLSA
Sbjct: 601 SYTSTNALTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGIDIKETKEKTLSA 632

[Close]

BLAST of MS019122 vs. ExPASy TrEMBL
Match: A0A6J1F3K1 (2-isopropylmalate synthase OS=Cucurbita moschata OX=3662 GN=LOC111441856 PE=3 SV=1)

HSP 1 Score: 1074.7 bits (2778), Expect = 2.4e-310
Identity = 555/656 (84.60%), Postives = 583/656 (88.87%), Query Frame = 0


Query: 1   MAIPTAFSRPNHYFARQSNSQFPTCRQIYGRNLQFGNAVDSCPLSFRSAAGRVSRVYCCQ 60
           MA+P A SRPN +  RQ NSQF    +IY RNLQ      S  LSFR+A   VSRVYCCQ
Sbjct: 1   MAMPAALSRPNQFLVRQPNSQFSARHRIYSRNLQ-ALKNPSRSLSFRTATDHVSRVYCCQ 60

Query: 61  AQSIASDSKPNSSVRRPPYIPNRISDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQL 120
            + +  +S+  SS+RRP YIPNRI D SYVRIFDTTLRDGEQSPGASLT+KEKLDIARQL
Sbjct: 61  TERVVLNSESKSSIRRPSYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKLDIARQL 120

Query: 121 AKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNAVDQDGYVPVICGLSRCNENDIRTAWEA 180
           AKLGVDIIEAGFPAASKEDFEAVK+IAKEVGNAVD DGYVPVICGLSRCNE DIRTAWEA
Sbjct: 121 AKLGVDIIEAGFPAASKEDFEAVKMIAKEVGNAVDADGYVPVICGLSRCNEKDIRTAWEA 180

Query: 181 VKYAKRPRIHTFIATSEIHMKHKLRKTREQVIEIARSMVRFARSLGCDDVEFSPEDAGRS 240
           VKY+KRPRIHTFIATSEIHM+HKLRKTREQVIEIAR+MV+FARSLGCDDVEFSPEDAGRS
Sbjct: 181 VKYSKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVQFARSLGCDDVEFSPEDAGRS 240

Query: 241 DREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKSNTPGIENVIISTHCQND 300
           DREFLY+ILGEVIKAGATTLNIPDTVGYTMP EFG LIADIKSNTPGIENVIISTHCQND
Sbjct: 241 DREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISTHCQND 300

Query: 301 LGLATANTVAGACSGARQVEVTINGIGERAGNASLEEASYLFNLIYFYYFIYSEIIMILN 360
           LGLATANTVAGAC+GARQVEVTINGIGERAGNASLEE                       
Sbjct: 301 LGLATANTVAGACAGARQVEVTINGIGERAGNASLEE----------------------- 360

Query: 361 YKFVVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESG 420
              VVMALQCRGEHVLGGLH+GINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESG
Sbjct: 361 ---VVMALQCRGEHVLGGLHTGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESG 420

Query: 421 IHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDN 480
           IHQDGMLKHKGTYEII+PEDIGYERSNDAGIVLGKLSGRHALKSRL ELGYELDNE LDN
Sbjct: 421 IHQDGMLKHKGTYEIIAPEDIGYERSNDAGIVLGKLSGRHALKSRLVELGYELDNEKLDN 480

Query: 481 IFWRFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDAD 540
           +FWRFK VAEQKKRVTDAD+RALVSDEVFQP V+WKLLDLQVTCGTLGLSTATVKLLDAD
Sbjct: 481 VFWRFKAVAEQKKRVTDADIRALVSDEVFQPIVLWKLLDLQVTCGTLGLSTATVKLLDAD 540

Query: 541 GKEHINCAVGTGPVDSAYKAVDGIVKETVTLLEYSMNAVTEGIDAIATTRVVIRGENSYT 600
           G+EHI CAVGTGPVDSAYKAVD IVKE  TLLEYSM AVTEGIDAIATTRV+IRGE SYT
Sbjct: 541 GEEHIACAVGTGPVDSAYKAVDLIVKEPATLLEYSMTAVTEGIDAIATTRVLIRGEKSYT 600

Query: 601 STNAMTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKVTEKRTLSA 657
           +T+A TGE+VQRTFSGIGAGMDIVVSSVKAYIGALNKMLGF GVDIKVTEK+TLSA
Sbjct: 601 ATHASTGESVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFHGVDIKVTEKKTLSA 629

[Close]

BLAST of MS019122 vs. TAIR 10
Match: AT1G74040.1 (2-isopropylmalate synthase 1 )

HSP 1 Score: 916.4 bits (2367), Expect = 1.3e-266
Identity = 462/580 (79.66%), Postives = 517/580 (89.14%), Query Frame = 0


Query: 63  SIASDSKPNSSVRRPPYIPNRISDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQLAK 122
           S +S   P++  RRP YIPNRISDP+YVRIFDTTLRDGEQSPGA+LTSKEKLDIARQLAK
Sbjct: 60  SASSPLPPHAPRRRPNYIPNRISDPNYVRIFDTTLRDGEQSPGATLTSKEKLDIARQLAK 119

Query: 123 LGVDIIEAGFPAASKEDFEAVKIIAKEVGNAVDQDGYVPVICGLSRCNENDIRTAWEAVK 182
           LGVDIIEAGFPAASK+DFEAVK IA+ VGN VD++GYVPVICGLSRCN+ DI TAWEAVK
Sbjct: 120 LGVDIIEAGFPAASKDDFEAVKTIAETVGNTVDENGYVPVICGLSRCNKKDIETAWEAVK 179

Query: 183 YAKRPRIHTFIATSEIHMKHKLRKTREQVIEIARSMVRFARSLGCDDVEFSPEDAGRSDR 242
           YAKRPRIHTFIATS+IH+K+KL+K++E+VIEIAR+MVRFARSLGC+DVEFSPEDAGRS+R
Sbjct: 180 YAKRPRIHTFIATSDIHLKYKLKKSKEEVIEIARNMVRFARSLGCEDVEFSPEDAGRSER 239

Query: 243 EFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKSNTPGIENVIISTHCQNDLG 302
           E+LYEILGEVIKAGATTLNIPDTVG T+P EFG LIADIK+NTPGI+NVIISTHCQNDLG
Sbjct: 240 EYLYEILGEVIKAGATTLNIPDTVGITLPSEFGQLIADIKANTPGIQNVIISTHCQNDLG 299

Query: 303 LATANTVAGACSGARQVEVTINGIGERAGNASLEEASYLFNLIYFYYFIYSEIIMILNYK 362
           L+TANT++GA SGARQVEVTINGIGERAGNASLEE                         
Sbjct: 300 LSTANTLSGAHSGARQVEVTINGIGERAGNASLEE------------------------- 359

Query: 363 FVVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIH 422
            VVMA++CRG+HVLGGL +GI++RHI +TSKMVEEYTG+  QPHKAIVGANAFAHESGIH
Sbjct: 360 -VVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGMQTQPHKAIVGANAFAHESGIH 419

Query: 423 QDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIF 482
           QDGMLKHKGTYEI+SPE+IG ERSNDAGIVLGKLSGRHALK RL ELGY LD+  L N+F
Sbjct: 420 QDGMLKHKGTYEIMSPEEIGLERSNDAGIVLGKLSGRHALKDRLNELGYVLDDGQLSNLF 479

Query: 483 WRFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDADGK 542
           WRFK VAEQKKRVTDADL ALVSDEVFQP  VWKLLD+Q+TCGTLGLST+TVKL D+DGK
Sbjct: 480 WRFKAVAEQKKRVTDADLIALVSDEVFQPEAVWKLLDMQITCGTLGLSTSTVKLADSDGK 539

Query: 543 EHINCAVGTGPVDSAYKAVDGIVKETVTLLEYSMNAVTEGIDAIATTRVVIRGENSYTST 602
           EH+ C+VGTGPVD+AYKAVD IVKE  TLLEYSMNAVTEGIDAIATTRV+IRG+N+Y+ST
Sbjct: 540 EHVACSVGTGPVDAAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDNNYSST 599

Query: 603 NAMTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQ 643
           NA+TGE+V+RTFSG GAGMDIVVSSVKAY+GALNKMLGF+
Sbjct: 600 NAVTGESVERTFSGTGAGMDIVVSSVKAYVGALNKMLGFK 613

[Close]

BLAST of MS019122 vs. TAIR 10
Match: AT1G18500.1 (methylthioalkylmalate synthase-like 4 )

HSP 1 Score: 902.9 bits (2332), Expect = 1.5e-262
Identity = 452/567 (79.72%), Postives = 503/567 (88.71%), Query Frame = 0


Query: 76  RPPYIPNRISDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQLAKLGVDIIEAGFPAA 135
           RP YIPNRISDP+YVR+FDTTLRDGEQSPGA+LTSKEKLDIARQLAKLGVDIIEAGFPAA
Sbjct: 75  RPEYIPNRISDPNYVRVFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAA 134

Query: 136 SKEDFEAVKIIAKEVGNAVDQDGYVPVICGLSRCNENDIRTAWEAVKYAKRPRIHTFIAT 195
           SK+DFEAVK IA+ VGN VD++GYVPVICGLSRCN+ DI  AW+AVKYAKRPRIHTFIAT
Sbjct: 135 SKDDFEAVKTIAETVGNTVDENGYVPVICGLSRCNKKDIERAWDAVKYAKRPRIHTFIAT 194

Query: 196 SEIHMKHKLRKTREQVIEIARSMVRFARSLGCDDVEFSPEDAGRSDREFLYEILGEVIKA 255
           S+IH+++KL+KT+ +VIEIARSMVRFARSLGC+DVEFSPEDAGRS+RE+LYEILGEVIKA
Sbjct: 195 SDIHLEYKLKKTKAEVIEIARSMVRFARSLGCEDVEFSPEDAGRSEREYLYEILGEVIKA 254

Query: 256 GATTLNIPDTVGYTMPVEFGALIADIKSNTPGIENVIISTHCQNDLGLATANTVAGACSG 315
           GATTLNIPDTVG T+P EFG LI D+K+NTPGIENV+ISTHCQNDLGL+TANT++GA +G
Sbjct: 255 GATTLNIPDTVGITLPSEFGQLITDLKANTPGIENVVISTHCQNDLGLSTANTLSGAHAG 314

Query: 316 ARQVEVTINGIGERAGNASLEEASYLFNLIYFYYFIYSEIIMILNYKFVVMALQCRGEHV 375
           ARQ+EVTINGIGERAGNASLEE                          VVMA++CRG+HV
Sbjct: 315 ARQMEVTINGIGERAGNASLEE--------------------------VVMAIKCRGDHV 374

Query: 376 LGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEI 435
           LGGL +GI++RHI +TSKMVEEYTG+  QPHKAIVGANAFAHESGIHQDGMLKHKGTYEI
Sbjct: 375 LGGLFTGIDTRHIVMTSKMVEEYTGMQTQPHKAIVGANAFAHESGIHQDGMLKHKGTYEI 434

Query: 436 ISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRFKEVAEQKKRV 495
           I PE+IG ERSNDAGIVLGKLSGRHALK RL ELGY+LD+E L  IFWRFK VAEQKKRV
Sbjct: 435 ICPEEIGLERSNDAGIVLGKLSGRHALKDRLTELGYQLDDEQLSTIFWRFKTVAEQKKRV 494

Query: 496 TDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDADGKEHINCAVGTGPVD 555
           TDAD+ ALVSDEVFQP  VWKLLD+Q+TCGTLGLSTATVKL DADGKEH+ C++GTGPVD
Sbjct: 495 TDADIIALVSDEVFQPEAVWKLLDIQITCGTLGLSTATVKLADADGKEHVACSIGTGPVD 554

Query: 556 SAYKAVDGIVKETVTLLEYSMNAVTEGIDAIATTRVVIRGENSYTSTNAMTGEAVQRTFS 615
           SAYKAVD IVKE  TLLEYSMNAVTEGIDAIATTRV+IRG N Y+STNA+TGE VQRTFS
Sbjct: 555 SAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGSNKYSSTNAITGEEVQRTFS 614

Query: 616 GIGAGMDIVVSSVKAYIGALNKMLGFQ 643
           G GAGMDIVVSSVKAY+GALNKM+ F+
Sbjct: 615 GTGAGMDIVVSSVKAYVGALNKMMDFK 615

[Close]

BLAST of MS019122 vs. TAIR 10
Match: AT5G23010.1 (methylthioalkylmalate synthase 1 )

HSP 1 Score: 533.9 bits (1374), Expect = 1.8e-151
Identity = 274/469 (58.42%), Postives = 352/469 (75.05%), Query Frame = 0


Query: 43  PLSFRSAAGRVSRVYCCQAQSIASDSKPNSSVRRPPYIPNRISDPSYVRIFDTTLRDGEQ 102
           P  F S    VS+     A + ++D KP    R P YIPN++ D +YVR+FDTTLRDGEQ
Sbjct: 43  PALFISCCSSVSK----NAATSSTDLKPVVE-RWPEYIPNKLPDGNYVRVFDTTLRDGEQ 102

Query: 103 SPGASLTSKEKLDIARQLAKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNAVDQD-GYVP 162
           SPG SLT  +KL+IARQLAKL VDI+E GFP +S+E+ E +K IAK VGN VD++ GYVP
Sbjct: 103 SPGGSLTPPQKLEIARQLAKLRVDIMEVGFPGSSEEELETIKTIAKTVGNEVDEETGYVP 162

Query: 163 VICGLSRCNENDIRTAWEAVKYAKRPRIHTFIATSEIHMKHKLRKTREQVIEIARSMVRF 222
           VIC ++RC   DI   WEA+KYAKRPRI  F +TS+IHMK+KL+KT+E+VIE+A S +RF
Sbjct: 163 VICAIARCKHRDIEATWEALKYAKRPRILVFTSTSDIHMKYKLKKTQEEVIEMAVSSIRF 222

Query: 223 ARSLGCDDVEFSPEDAGRSDREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADI 282
           A+SLG +D++F  ED GRSD++FL +ILGE IKAG T + I DTVG  MP E+G L+  +
Sbjct: 223 AKSLGFNDIQFGCEDGGRSDKDFLCKILGEAIKAGVTVVTIGDTVGINMPHEYGELVTYL 282

Query: 283 KSNTPGIENVIISTHCQNDLGLATANTVAGACSGARQVEVTINGIGERAGNASLEEASYL 342
           K+NTPGI++V+++ HC NDLGLATAN++AG  +GARQVEVTINGIGER+GNASLEE    
Sbjct: 283 KANTPGIDDVVVAVHCHNDLGLATANSIAGIRAGARQVEVTINGIGERSGNASLEE---- 342

Query: 343 FNLIYFYYFIYSEIIMILNYKFVVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGL 402
                                 VVMAL+CRG +V+ G+++ I++R I  TSKMV+EYTGL
Sbjct: 343 ----------------------VVMALKCRGAYVINGVYTKIDTRQIMATSKMVQEYTGL 402

Query: 403 NVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHA 462
            VQ HK IVGAN F HESGIHQDG+LK++ TYEI+SPEDIG  +S ++G+VLGKLSGRHA
Sbjct: 403 YVQAHKPIVGANCFVHESGIHQDGILKNRSTYEILSPEDIGIVKSQNSGLVLGKLSGRHA 462

Query: 463 LKSRLQELGYELDNENLDNIFWRFKEVAEQKKRVTDADLRALV--SDEV 509
           +K RL+ELGYELD+E L+ +F  F+++ + KKR+TDADL+ALV  SDE+
Sbjct: 463 VKDRLKELGYELDDEKLNAVFSLFRDLTKNKKRITDADLKALVTSSDEI 480

[Close]

BLAST of MS019122 vs. TAIR 10
Match: AT5G23020.1 (2-isopropylmalate synthase 2 )

HSP 1 Score: 533.1 bits (1372), Expect = 3.1e-151
Identity = 265/455 (58.24%), Postives = 347/455 (76.26%), Query Frame = 0


Query: 56  VYCCQAQS-----IASDSKPNSSVRRPPYIPNRISDPSYVRIFDTTLRDGEQSPGASLTS 115
           V CC A+S      A+D KP    RRP YIPN++   +YVR+ DTTLRDGEQSPGA+LT 
Sbjct: 47  VSCCSAESKKVATSATDLKPIME-RRPEYIPNKLPHKNYVRVLDTTLRDGEQSPGAALTP 106

Query: 116 KEKLDIARQLAKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNAVDQD-GYVPVICGLSRC 175
            +KL+IARQLAKL VDI+E GFP +S+E+FEA+K IAK VGN VD++ GYVPVICG++RC
Sbjct: 107 PQKLEIARQLAKLRVDIMEVGFPVSSEEEFEAIKTIAKTVGNEVDEETGYVPVICGIARC 166

Query: 176 NENDIRTAWEAVKYAKRPRIHTFIATSEIHMKHKLRKTREQVIEIARSMVRFARSLGCDD 235
            + DI   WEA+KYAKRPR+  F +TSEIHMK+KL+KT+E+VIE+A + V++A+SLG  D
Sbjct: 167 KKRDIEATWEALKYAKRPRVMLFTSTSEIHMKYKLKKTKEEVIEMAVNSVKYAKSLGFKD 226

Query: 236 VEFSPEDAGRSDREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKSNTPGIE 295
           ++F  ED GR++++F+ +ILGE IKAGATT+   DTVG  MP EFG L+A +  NTPG +
Sbjct: 227 IQFGCEDGGRTEKDFICKILGESIKAGATTVGFADTVGINMPQEFGELVAYVIENTPGAD 286

Query: 296 NVIISTHCQNDLGLATANTVAGACSGARQVEVTINGIGERAGNASLEEASYLFNLIYFYY 355
           +++ + HC NDLG+ATANT++G C+GARQVEVTINGIGER+GNA LEE            
Sbjct: 287 DIVFAIHCHNDLGVATANTISGICAGARQVEVTINGIGERSGNAPLEE------------ 346

Query: 356 FIYSEIIMILNYKFVVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAI 415
                         VVMAL+CRGE ++ G+++ I+SR I  TSKMV+E+TG+ VQPHK I
Sbjct: 347 --------------VVMALKCRGESLMDGVYTKIDSRQIMATSKMVQEHTGMYVQPHKPI 406

Query: 416 VGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQEL 475
           VG N F HESGIHQDG+LK++ TYEI+SPED+G  +S ++GIVLGKLSGRHA+K RL+EL
Sbjct: 407 VGDNCFVHESGIHQDGILKNRSTYEILSPEDVGIVKSENSGIVLGKLSGRHAVKDRLKEL 466

Query: 476 GYELDNENLDNIFWRFKEVAEQKKRVTDADLRALV 505
           GYE+ +E  ++IF R++E+ + KKR+TDADL+ALV
Sbjct: 467 GYEISDEKFNDIFSRYRELTKDKKRITDADLKALV 474

The following BLAST results are available for this feature:

BLAST of MS019122 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141881.1	0.0e+00	89.53	2-isopropylmalate synthase 2, chloroplastic-like isoform X1 [Momordica charantia...	[more]
XP_022141882.1	0.0e+00	89.40	2-isopropylmalate synthase 2, chloroplastic-like isoform X2 [Momordica charantia...	[more]
XP_038875433.1	0.0e+00	85.69	2-isopropylmalate synthase 2, chloroplastic-like [Benincasa hispida]	[more]
XP_022984140.1	0.0e+00	84.91	2-isopropylmalate synthase 2, chloroplastic-like [Cucurbita maxima]	[more]
TYK01483.1	0.0e+00	84.22	2-isopropylmalate synthase 2 [Cucumis melo var. makuwa]	[more]

BLAST of MS019122 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C550	1.9e-265	79.66	2-isopropylmalate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=I...	[more]
Q9LPR4	2.1e-261	79.72	2-isopropylmalate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=I...	[more]
O04973	3.1e-244	73.51	2-isopropylmalate synthase A OS=Solanum pennellii OX=28526 GN=IPMSA PE=2 SV=1	[more]
O04974	1.8e-239	72.68	2-isopropylmalate synthase B OS=Solanum pennellii OX=28526 GN=IPMSB PE=2 SV=1	[more]
Q39891	2.4e-212	66.03	Probable 2-isopropylmalate synthase OS=Glycine max OX=3847 GN=GMN56 PE=2 SV=1	[more]

BLAST of MS019122 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CKJ3	0.0e+00	89.53	2-isopropylmalate synthase OS=Momordica charantia OX=3673 GN=LOC111012138 PE=3 S...	[more]
A0A6J1CL37	0.0e+00	89.40	2-isopropylmalate synthase OS=Momordica charantia OX=3673 GN=LOC111012138 PE=3 S...	[more]
A0A6J1J7V5	0.0e+00	84.91	2-isopropylmalate synthase OS=Cucurbita maxima OX=3661 GN=LOC111482524 PE=3 SV=1	[more]
A0A5D3BP53	0.0e+00	84.22	2-isopropylmalate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A6J1F3K1	2.4e-310	84.60	2-isopropylmalate synthase OS=Cucurbita moschata OX=3662 GN=LOC111441856 PE=3 SV...	[more]

BLAST of MS019122 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G74040.1	1.3e-266	79.66	2-isopropylmalate synthase 1	[more]
AT1G18500.1	1.5e-262	79.72	methylthioalkylmalate synthase-like 4	[more]
AT5G23010.1	1.8e-151	58.42	methylthioalkylmalate synthase 1	[more]
AT5G23020.1	3.1e-151	58.24	2-isopropylmalate synthase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013709	2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain	SMART	SM00917	LeuA_dimer_2	coord: 494..639 e-value: 6.2E-28 score: 108.8
IPR013709	2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain	PFAM	PF08502	LeuA_dimer	coord: 495..639 e-value: 2.7E-35 score: 121.1
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 76..355 e-value: 4.8E-99 score: 333.8
None	No IPR available	GENE3D	1.10.238.260		coord: 402..515 e-value: 6.6E-38 score: 131.6
None	No IPR available	PANTHER	PTHR10277	HOMOCITRATE SYNTHASE-RELATED	coord: 81..338
None	No IPR available	PANTHER	PTHR10277:SF9	2-ISOPROPYLMALATE SYNTHASE 1, CHLOROPLASTIC	coord: 81..338
None	No IPR available	PANTHER	PTHR10277:SF9	2-ISOPROPYLMALATE SYNTHASE 1, CHLOROPLASTIC	coord: 364..643
None	No IPR available	PANTHER	PTHR10277	HOMOCITRATE SYNTHASE-RELATED	coord: 364..643
None	No IPR available	CDD	cd07940	DRE_TIM_IPMS	coord: 92..397 e-value: 3.76911E-172 score: 489.267
None	No IPR available	SUPERFAMILY	51569	Aldolase	coord: 86..343
IPR000891	Pyruvate carboxyltransferase	PFAM	PF00682	HMGL-like	coord: 90..337 e-value: 4.7E-88 score: 295.1
IPR000891	Pyruvate carboxyltransferase	PROSITE	PS50991	PYR_CT	coord: 90..357 score: 38.664776
IPR036230	2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain superfamily	GENE3D	3.30.160.270		coord: 524..639 e-value: 1.4E-23 score: 85.1
IPR036230	2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain superfamily	SUPERFAMILY	110921	2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain	coord: 518..638
IPR002034	Alpha-isopropylmalate/homocitrate synthase, conserved site	PROSITE	PS00815	AIPM_HOMOCIT_SYNTH_1	coord: 97..113
IPR005671	2-isopropylmalate synthase, bacterial-type	HAMAP	MF_01025	LeuA_type1	coord: 87..648 score: 38.921787

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS019122.1	MS019122.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019752	carboxylic acid metabolic process
biological_process	GO:0009098	leucine biosynthetic process
molecular_function	GO:0003852	2-isopropylmalate synthase activity
molecular_function	GO:0046912	acyltransferase, acyl groups converted into alkyl on transfer
molecular_function	GO:0003824	catalytic activity