MS019113 (gene) Bitter gourd (TR) v1

Overview
NameMS019113
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionATP-dependent Clp protease proteolytic subunit
Locationscaffold20: 1573517 .. 1577088 (+)
RNA-Seq ExpressionMS019113
SyntenyMS019113
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGATGAGTTTCACATACACGCCTTCTCCTTCGCCTCAGAGGCTCATGCGCTTCGACCATGGAGTTCTAAGTTCTCAGTTTCCACTTTTCACTCCTCGGAAGCACTCCCATGGCGGCAATTCCAGTTTAAATGGTCTTAGGACCCCAAAATCGGCTCCTCTAGTGAAGGCCTCGCTCAGTTCTTCGTCGATGAGGCGGACCTTATCAAGTAATTGGGACGTTTTGGAGAATTTTTCTGCAACTTCCGCTCCATCGCTGGCTAGGTTCGAAGAGCTCGACACTACCAACATGCTTCTCCGCCAGCGAATCATCTTCTTGGGCTCTCAGGTAGTCTCCCTCTCTCTCTCTGTGAAACAAGATTCCGATTCCTGATTTCTGGACCAGTTGTGTTGAATATTTTTGCAGCTTTCTTAGGTTTAAATTGTGAGGTTTTGCTTTTTTCATTATGTTGAATTGGTTTTGTTTGGATGATTTGTGAATTGTTTAACTTGACTTGTAGACTACCTGTTACTGTTAGTGATCTATTGGTGCTACTGGCAGGTTGATGACATGACAGCAGATTTCATCATAAGCCAGCTACTGTTTCTTGATGCTGAAGACTCAACAAAAGACATCAAATTGTTTATCAACTCACCTGGTGGCTCTGTCACTGCTGGTATTTTTAGCATCATTTAATTTTCATCATGAAATCCTTGATTAATATTTTTAGAGTGATAAATTATAGTTCTGATACGAAGTTGTTGGAAGGGTGATCTCGTAAAAATTAAAATCTTTATTGTTCACACCTGCTTTCCACGGAATCCCTTCCCTTCCCTTCATTCTTCTAGGCTGGTTTTAGCTTTGGCCTCAAATAGGCTTCTTATCACTCTGTAAGCACGTATATGTAGAGTGTGAAGTATATTGCTAAATCCACATATATATTTTTTCACTTCAAAATGAGATCATGGCGCCTTATGTGTTGATCAAATGCAGATAATAGAATGGCAATTCTCTTTTAGCTTTCCATCCTTTTAAATCGGAATGTCGGAATGTAGATCTCAATGCAAGATAAGCATATTTCAAAATTTTGGAATCATTGAAACATTTGAGATTCAGGTCCTAATATGTTGTGCTTTGCTTTGACTTGATTATTCTCACTATGGAAGTAGCTTGGGAAGTGTCATATCTGAGCCAGGATCATAATCCGTCTAGTTGTGAGCCAAGAGTGTCCGTTAGTGGTTGTGTTATTGTATTTTTGTTTTATGATAGTTTAATTCATTCATGTATTAGAGAGAACAGTGTGACATGCTTAGTTTTCAAAAAATATTCTTATTTTCTCTTCCATGTTCAGTTAAACTAGTTATTACTTGTTACCACTATAGTTGTAACTTTTGCCATCAACCAAATGAAAAGTTTAGAATGTGGTGTGGCAATCATGGGGCAAGAGTTTGGATGATATTCATATTACCTGATTCAATTTCATTTCGCATGAAATAATATATTTTCTGATTAGGAGGGTTGCATAAACATTCCCTTGCTGTGGGTTGGTTTCTTTCCTTCAACCGGCTTAAGAATTATATTACTGAAAATTGTGCAATGCCCATTCTGTCTAGTGAGTTCGACTTTCCTCCCCGTTGCCTCGTTATTTGTAAGCTGTAACTAGCATATGAACCCACTTGCCTCCCCAAGCATTTACCTCTTCTATCGTTTTCCCTCACTAAATGCAGCCCTTATTATGTTCGTTTATCTTCTGTTCATCTCCTCCTTCTTCAATCATTTCAAGGCCTTCTCAAATTGAGCTTTGGAGTTCCACTTTTTCTTTCGGTCTACCTCTAATGTTGTGTGTTGTTTTTCATCCATATATGTTTCTCAACATTTTGTGGCTGCATGTAGGAATGGGAATATACGATGCAATGAAGTTGTGCAAGGCCGACGTTTCAACAGTTTGTCTAGGACTCGCTGCATCTATGGGAGCATTTCTTCTAGCTGCTGGTTCAAAGGGAAAGCGATTCTGCATGCCCAATGGGAGAGTGATGATCCATCAACCTCTCGGAACAGCTGGAGGGAAGGTGAGTACACTATTATTTTCTATGGGGTTGATGTTAAAAGTTAGATCATAAAGTTCATAGCTCGTTTTCCACCGTGATTGCATTTTTTTGTTTCACCTTCATTTTATTTACTTTTCTAGTCCCGGTTGAACTTGCTAAATATCTAATATTATTCTACGTTCATTCTATTAAGTGGCTGTGGAAACATGCAGATATATGTTTATGAGGATTTGAAGTAGTTGTTGGAATTGTATGATGGCCATAGGGTATCTAGATCAACAGCCATCTGAAAATACCAAAAATTTCTTCCAATTAAGTAATTCTTGAGTATTGCATAATCTAGTTGTTATAAACTTGAGTTATTTTGTGTGTGAACTTATTGATGGGAACATACCAGCCGTGCAGCATGTGTATTGTGTATACCCGAAGAGAGGACAGCAAAAAGAAGAAAGAGAGTTAGTTATGCTTTTTTAGAGTACGTGCACCAGAGTGAGCCCCATTTTTGAAATAGGTCGGACACGGCCATGGGTTGTGTGGGTGGGTTTATCTATGGGTTTAGGATCTCTTTTTACGCATCTGAGAATTCCGTTAGTATTTTGGCCTTGGGGAATATTTTTCCTTGAGGAGGGAGAGTTCTTGGCCTCTCAAAGAGCTAAGGTTACTGGCTGTTTGCGCCCTGATTTGGGATGATTAGTTGAATATATTGGAAATAGGCCATTTGAGGCCTATCACTTGTTTTTTTTTTTTTTAGTCCAAAAGGTGGGGTGGGGGATCGAACTCACGACCTCTTGGTTTTGGAGATATATGTCAATTACTGCTGAGCTATGCTCGCTTTGGGAAGGCCTATCACTGGTTTACAAGGACGCAATTGGCATTCTTTAGCTCGCCCACCTAGAGTGGGTATAAGGGCATAGAATCAATTATCAAATGCAGGAACTTTTCCCAAATTTTATTTATAACTATTAATTTGCCCTGTTGATCTATATAATATGTAAATTTGAGCTTCCACTTATGGTAGTTCTCCCCTAATCACTTTGTAATTCATTGGTTACCCCGTTGTCCATGCCAGGCAACAGAGATGAGTATACGGATACGAGAAATGGCGTATCACAAGATCAAGCTGAACAAAATTTTTTCAAGAATCACAGGAAAGCCATTAGAGCAGGTTTTGCCTCTACATTGTTTCTTTAACTCTTCCCGGTTCTCAGTTTCTTTCTGAAACCCATGAAATTTATGAGTTGTCATTTGCAGATTGAATTGGACACGGACCGCGATAATTTCATGAATCCCTGGGAAGCTAAGGAATATGGTTTGATTGATGAAGTTATTGATGATGGGAAGCCAGGACTCATTGCTCCAATTGCAGATGCAGCTGCTCCGCCAAAAACCCGAGTCTGGGATCTATGGAAGGTGGAAGGGAGTAGGAAAGCCAAGAAGAATCTGCCTTCAGAAGACAAGATCTTGCAGAATGGATATCAAGGGGGTCAAGGAAACGACGAGGAGCGCGGTACCCAACAAGAAACAGAAGAACCGAGTCCTGTA

mRNA sequence

ATGGAGATGAGTTTCACATACACGCCTTCTCCTTCGCCTCAGAGGCTCATGCGCTTCGACCATGGAGTTCTAAGTTCTCAGTTTCCACTTTTCACTCCTCGGAAGCACTCCCATGGCGGCAATTCCAGTTTAAATGGTCTTAGGACCCCAAAATCGGCTCCTCTAGTGAAGGCCTCGCTCAGTTCTTCGTCGATGAGGCGGACCTTATCAAGTAATTGGGACGTTTTGGAGAATTTTTCTGCAACTTCCGCTCCATCGCTGGCTAGGTTCGAAGAGCTCGACACTACCAACATGCTTCTCCGCCAGCGAATCATCTTCTTGGGCTCTCAGGTAGTTGATGACATGACAGCAGATTTCATCATAAGCCAGCTACTGTTTCTTGATGCTGAAGACTCAACAAAAGACATCAAATTGTTTATCAACTCACCTGGTGGCTCTGTCACTGCTGGAATGGGAATATACGATGCAATGAAGTTGTGCAAGGCCGACGTTTCAACAGTTTGTCTAGGACTCGCTGCATCTATGGGAGCATTTCTTCTAGCTGCTGGTTCAAAGGGAAAGCGATTCTGCATGCCCAATGGGAGAGTGATGATCCATCAACCTCTCGGAACAGCTGGAGGGAAGGCAACAGAGATGAGTATACGGATACGAGAAATGGCGTATCACAAGATCAAGCTGAACAAAATTTTTTCAAGAATCACAGGAAAGCCATTAGAGCAGATTGAATTGGACACGGACCGCGATAATTTCATGAATCCCTGGGAAGCTAAGGAATATGGTTTGATTGATGAAGTTATTGATGATGGGAAGCCAGGACTCATTGCTCCAATTGCAGATGCAGCTGCTCCGCCAAAAACCCGAGTCTGGGATCTATGGAAGGTGGAAGGGAGTAGGAAAGCCAAGAAGAATCTGCCTTCAGAAGACAAGATCTTGCAGAATGGATATCAAGGGGGTCAAGGAAACGACGAGGAGCGCGGTACCCAACAAGAAACAGAAGAACCGAGTCCTGTA

Coding sequence (CDS)

ATGGAGATGAGTTTCACATACACGCCTTCTCCTTCGCCTCAGAGGCTCATGCGCTTCGACCATGGAGTTCTAAGTTCTCAGTTTCCACTTTTCACTCCTCGGAAGCACTCCCATGGCGGCAATTCCAGTTTAAATGGTCTTAGGACCCCAAAATCGGCTCCTCTAGTGAAGGCCTCGCTCAGTTCTTCGTCGATGAGGCGGACCTTATCAAGTAATTGGGACGTTTTGGAGAATTTTTCTGCAACTTCCGCTCCATCGCTGGCTAGGTTCGAAGAGCTCGACACTACCAACATGCTTCTCCGCCAGCGAATCATCTTCTTGGGCTCTCAGGTAGTTGATGACATGACAGCAGATTTCATCATAAGCCAGCTACTGTTTCTTGATGCTGAAGACTCAACAAAAGACATCAAATTGTTTATCAACTCACCTGGTGGCTCTGTCACTGCTGGAATGGGAATATACGATGCAATGAAGTTGTGCAAGGCCGACGTTTCAACAGTTTGTCTAGGACTCGCTGCATCTATGGGAGCATTTCTTCTAGCTGCTGGTTCAAAGGGAAAGCGATTCTGCATGCCCAATGGGAGAGTGATGATCCATCAACCTCTCGGAACAGCTGGAGGGAAGGCAACAGAGATGAGTATACGGATACGAGAAATGGCGTATCACAAGATCAAGCTGAACAAAATTTTTTCAAGAATCACAGGAAAGCCATTAGAGCAGATTGAATTGGACACGGACCGCGATAATTTCATGAATCCCTGGGAAGCTAAGGAATATGGTTTGATTGATGAAGTTATTGATGATGGGAAGCCAGGACTCATTGCTCCAATTGCAGATGCAGCTGCTCCGCCAAAAACCCGAGTCTGGGATCTATGGAAGGTGGAAGGGAGTAGGAAAGCCAAGAAGAATCTGCCTTCAGAAGACAAGATCTTGCAGAATGGATATCAAGGGGGTCAAGGAAACGACGAGGAGCGCGGTACCCAACAAGAAACAGAAGAACCGAGTCCTGTA

Protein sequence

MEMSFTYTPSPSPQRLMRFDHGVLSSQFPLFTPRKHSHGGNSSLNGLRTPKSAPLVKASLSSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQVVDDMTADFIISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAFLLAAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPLEQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSRKAKKNLPSEDKILQNGYQGGQGNDEERGTQQETEEPSPV
Homology
BLAST of MS019113 vs. NCBI nr
Match: XP_022143418.1 (ATP-dependent Clp protease proteolytic subunit 3, chloroplastic [Momordica charantia])

HSP 1 Score: 659.1 bits (1699), Expect = 2.1e-185
Identity = 336/337 (99.70%), Postives = 336/337 (99.70%), Query Frame = 0

Query: 1   MEMSFTYTPSPSPQRLMRFDHGVLSSQFPLFTPRKHSHGGNSSLNGLRTPKSAPLVKASL 60
           MEMSFTYTPSPSPQRLMRFDHGVLSSQFPLFTPRKHSHGGNSSLNGLRTPKSAPLVKASL
Sbjct: 1   MEMSFTYTPSPSPQRLMRFDHGVLSSQFPLFTPRKHSHGGNSSLNGLRTPKSAPLVKASL 60

Query: 61  SSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQVVDDMTADFI 120
           SSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQ VDDMTADFI
Sbjct: 61  SSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQ-VDDMTADFI 120

Query: 121 ISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAFLL 180
           ISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAFLL
Sbjct: 121 ISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAFLL 180

Query: 181 AAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPLEQ 240
           AAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPLEQ
Sbjct: 181 AAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPLEQ 240

Query: 241 IELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSRKA 300
           IELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSRKA
Sbjct: 241 IELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSRKA 300

Query: 301 KKNLPSEDKILQNGYQGGQGNDEERGTQQETEEPSPV 338
           KKNLPSEDKILQNGYQGGQGNDEERGTQQETEEPSPV
Sbjct: 301 KKNLPSEDKILQNGYQGGQGNDEERGTQQETEEPSPV 336

BLAST of MS019113 vs. NCBI nr
Match: XP_038902354.1 (ATP-dependent Clp protease proteolytic subunit 3, chloroplastic [Benincasa hispida])

HSP 1 Score: 574.7 bits (1480), Expect = 5.2e-160
Identity = 290/337 (86.05%), Postives = 308/337 (91.39%), Query Frame = 0

Query: 1   MEMSFTYTPSPSPQRLMRFDHGVLSSQFPLFTPRKHSHGGNSSLNGLRTPKSAPLVKASL 60
           ME+SFTY PS SP RLMR DHG+ SS FP+  P KHSHGGNS+ N  R   S P+V  S 
Sbjct: 1   MELSFTYAPSSSPHRLMRIDHGLPSSYFPISIPSKHSHGGNSTSNAFRIRNSVPVVNPSF 60

Query: 61  SSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQVVDDMTADFI 120
           +SSS+RRTLSS+WDVL+N+SA+SAPSL RFEELDTTNMLLRQRIIFLGSQ VDDMTADFI
Sbjct: 61  NSSSLRRTLSSSWDVLDNYSASSAPSLPRFEELDTTNMLLRQRIIFLGSQ-VDDMTADFI 120

Query: 121 ISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAFLL 180
           ISQLLFLDAED TKDIKLFINSPGGSVTAGMGIYDAMKLCKADVST+CLGLAASMGAFLL
Sbjct: 121 ISQLLFLDAEDPTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTICLGLAASMGAFLL 180

Query: 181 AAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPLEQ 240
           A+GSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREM YHKIKLNKIFSRITGKPLEQ
Sbjct: 181 ASGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMTYHKIKLNKIFSRITGKPLEQ 240

Query: 241 IELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSRKA 300
           IELDTDRDNFMNPWEAK+YGLIDEVIDDGKPGLIAP A+A  PPKTRVWDLWKVEGSRK 
Sbjct: 241 IELDTDRDNFMNPWEAKDYGLIDEVIDDGKPGLIAPTAEATPPPKTRVWDLWKVEGSRKT 300

Query: 301 KKNLPSEDKILQNGYQGGQGNDEERGTQQETEEPSPV 338
           KKNLPSE+KILQNGYQ GQG+DEERG+QQETEE SPV
Sbjct: 301 KKNLPSENKILQNGYQEGQGSDEERGSQQETEETSPV 336

BLAST of MS019113 vs. NCBI nr
Match: XP_008457103.1 (PREDICTED: ATP-dependent Clp protease proteolytic subunit 3, chloroplastic [Cucumis melo])

HSP 1 Score: 562.0 bits (1447), Expect = 3.5e-156
Identity = 291/340 (85.59%), Postives = 309/340 (90.88%), Query Frame = 0

Query: 1   MEMSFTYTPSPSPQRLMRFDHGV-LSSQFPLFTPRKHSHGGNSSLNGLRTPK-SAPLVK- 60
           ME+SFT+ PSPSP  L RFDHG+  SS  P+ TP  HSHG N + N  R    S P++  
Sbjct: 1   MELSFTHAPSPSPYGLKRFDHGLHHSSHVPISTPSNHSHGRNFTSNNFRIRNCSVPVLNH 60

Query: 61  ASLSSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQVVDDMTA 120
            SLSSSS+RRTLSSNWDVL N+SA+SAPSL RFEELDTTNMLLRQRIIFLGSQ VDD+TA
Sbjct: 61  PSLSSSSLRRTLSSNWDVLSNYSASSAPSLPRFEELDTTNMLLRQRIIFLGSQ-VDDVTA 120

Query: 121 DFIISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGA 180
           DFIISQLLFLDAED TKDIKLFINSPGGSVTAGMGIYDAMKLCKADVST+CLGLAASMGA
Sbjct: 121 DFIISQLLFLDAEDPTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTICLGLAASMGA 180

Query: 181 FLLAAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKP 240
           FLLA+GSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKP
Sbjct: 181 FLLASGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKP 240

Query: 241 LEQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGS 300
           LEQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIA+A  PPKTRVWDLWKVEGS
Sbjct: 241 LEQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIAEATPPPKTRVWDLWKVEGS 300

Query: 301 RKAKKNLPSEDKILQNGYQGGQGNDEERGTQQETEEPSPV 338
           RKAKKNLPSE KIL+NGYQGG+G+DEERG+QQETEEPSPV
Sbjct: 301 RKAKKNLPSESKILKNGYQGGEGSDEERGSQQETEEPSPV 339

BLAST of MS019113 vs. NCBI nr
Match: XP_022948597.1 (ATP-dependent Clp protease proteolytic subunit 3, chloroplastic [Cucurbita moschata])

HSP 1 Score: 554.7 bits (1428), Expect = 5.6e-154
Identity = 290/339 (85.55%), Postives = 305/339 (89.97%), Query Frame = 0

Query: 1   MEMSFTYTPSPS-PQRLMRFDHGVLSSQFPLFTPRKHSHGGNSSLNGLRTPKSAPL--VK 60
           ME+SFTY PSPS   RLM F+HGVL+S FP+ TP KHSHG NSS N  R  KS P+  VK
Sbjct: 1   MELSFTYAPSPSLHSRLMCFNHGVLASPFPVSTPFKHSHGANSSSNAFRIRKSVPVAAVK 60

Query: 61  ASLSSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQVVDDMTA 120
           ASLSSS +RRTLSSNWDVL N+SA SAP+L RFEELDTTNMLLRQRIIFLGSQ VDDMTA
Sbjct: 61  ASLSSSPLRRTLSSNWDVLGNYSAASAPALPRFEELDTTNMLLRQRIIFLGSQ-VDDMTA 120

Query: 121 DFIISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGA 180
           DFIISQLLFLDAED TKDIKL INSPGGSVTAGMGIYDAMKLCKADVSTVC+GLAASMGA
Sbjct: 121 DFIISQLLFLDAEDPTKDIKLLINSPGGSVTAGMGIYDAMKLCKADVSTVCMGLAASMGA 180

Query: 181 FLLAAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKP 240
           FLLAAGSKGKRFCMPNGRVMIHQPLGTAGG+AT+MSIR+REMAYHKIKLNKI SRITGKP
Sbjct: 181 FLLAAGSKGKRFCMPNGRVMIHQPLGTAGGRATDMSIRLREMAYHKIKLNKILSRITGKP 240

Query: 241 LEQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGS 300
           LEQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIA+A  PPKTRVWDLWK+EGS
Sbjct: 241 LEQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIAEANPPPKTRVWDLWKIEGS 300

Query: 301 RKAKKNLPSEDKILQNGYQGGQGNDEERGTQQETEEPSP 337
           RKAKKNLPSE KILQNGY   QG+DEER  Q ETEEPSP
Sbjct: 301 RKAKKNLPSETKILQNGY---QGSDEERSPQPETEEPSP 335

BLAST of MS019113 vs. NCBI nr
Match: XP_011651427.1 (ATP-dependent Clp protease proteolytic subunit 3, chloroplastic [Cucumis sativus] >KGN57884.1 hypothetical protein Csa_010181 [Cucumis sativus])

HSP 1 Score: 554.7 bits (1428), Expect = 5.6e-154
Identity = 288/340 (84.71%), Postives = 305/340 (89.71%), Query Frame = 0

Query: 1   MEMSFTYTPSPSPQRLMRFDHGVLSSQFPLFTPRKHSHGGNSSLNGLRTPK-SAPLVK-A 60
           ME+SFT+ PSPSP  L R DHG  SS FP+ TP KHSHGGN + N  R    S P+V   
Sbjct: 1   MELSFTHAPSPSPYGLKRSDHGFQSSHFPISTPSKHSHGGNFTSNAFRIRNCSVPVVNHP 60

Query: 61  SLSSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQVVDDMTAD 120
           S SSSS+RRTLSSNWDV  N+S +SA SL RFEELDTTNMLLRQRIIFLGSQ VDDMTAD
Sbjct: 61  SHSSSSLRRTLSSNWDVFSNYSVSSARSLPRFEELDTTNMLLRQRIIFLGSQ-VDDMTAD 120

Query: 121 FIISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAF 180
           FIISQLLFLDAED TKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAF
Sbjct: 121 FIISQLLFLDAEDPTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAF 180

Query: 181 LLAAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPL 240
           LLA+GSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKP+
Sbjct: 181 LLASGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPI 240

Query: 241 EQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSR 300
           EQIELDT+RDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIA+A  PPKTR+WDLWKVEGSR
Sbjct: 241 EQIELDTERDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIAEATPPPKTRIWDLWKVEGSR 300

Query: 301 KAKKNLPSEDKILQNGY-QGGQGNDEERGTQQETEEPSPV 338
           KAKKNLPSE KIL+NGY +GGQG+D+ERG QQETEEPSPV
Sbjct: 301 KAKKNLPSESKILKNGYEEGGQGSDKERGGQQETEEPSPV 339

BLAST of MS019113 vs. ExPASy Swiss-Prot
Match: Q9SXJ6 (ATP-dependent Clp protease proteolytic subunit 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CLPP3 PE=1 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 7.2e-112
Identity = 218/306 (71.24%), Postives = 242/306 (79.08%), Query Frame = 0

Query: 17  MRFDHGVLSSQFPLFTPRKHSHGGNSSLNGLRTPKSAP--LVKASLS-SSSMRRTLSSNW 76
           +R      S+   L  P K+    N  +   R PK++    V++S+S S   R+TLSSNW
Sbjct: 5   LRLASSSTSNPICLLNPGKNL---NFPIRNHRIPKTSKPFCVRSSMSLSKPPRQTLSSNW 64

Query: 77  DVLENFS----ATSAPSLARFEELDTTNMLLRQRIIFLGSQVVDDMTADFIISQLLFLDA 136
           DV  +FS    A S   L  FEELDTTNMLLRQRI+FLGSQ VDDMTAD +ISQLL LDA
Sbjct: 65  DV-SSFSIDSVAQSPSRLPSFEELDTTNMLLRQRIVFLGSQ-VDDMTADLVISQLLLLDA 124

Query: 137 EDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAFLLAAGSKGKRF 196
           EDS +DI LFINSPGGS+TAGMGIYDAMK CKADVSTVCLGLAASMGAFLLA+GSKGKR+
Sbjct: 125 EDSERDITLFINSPGGSITAGMGIYDAMKQCKADVSTVCLGLAASMGAFLLASGSKGKRY 184

Query: 197 CMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPLEQIELDTDRDN 256
           CMPN +VMIHQPLGTAGGKATEMSIRIREM YHKIKLNKIFSRITGKP  +IE DTDRDN
Sbjct: 185 CMPNSKVMIHQPLGTAGGKATEMSIRIREMMYHKIKLNKIFSRITGKPESEIESDTDRDN 244

Query: 257 FMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSRKAKKNLPSEDK 316
           F+NPWEAKEYGLID VIDDGKPGLIAPI D   PPKT+VWDLWKVEG++K   NLPSE  
Sbjct: 245 FLNPWEAKEYGLIDAVIDDGKPGLIAPIGDGTPPPKTKVWDLWKVEGTKKDNTNLPSERS 304

BLAST of MS019113 vs. ExPASy Swiss-Prot
Match: Q8YXH5 (ATP-dependent Clp protease proteolytic subunit 1 OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=clpP1 PE=3 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 3.6e-55
Identity = 109/174 (62.64%), Postives = 138/174 (79.31%), Query Frame = 0

Query: 94  DTTNMLLRQRIIFLGSQVVDDMTADFIISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGI 153
           D  + LLR+RI+FLG Q VD   A+ I++QLLFLDAED  KDI L+INSPGGSVTAGMGI
Sbjct: 18  DIYSRLLRERIVFLGQQ-VDSNLANLIVAQLLFLDAEDPEKDIYLYINSPGGSVTAGMGI 77

Query: 154 YDAMKLCKADVSTVCLGLAASMGAFLLAAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMS 213
           +D MK  + DV T+C GLAASMGAFLL+AG+KGKR  +P+ R+MIHQPLG A G+AT++ 
Sbjct: 78  FDTMKHIRPDVCTICTGLAASMGAFLLSAGAKGKRMSLPHSRIMIHQPLGGAQGQATDIE 137

Query: 214 IRIREMAYHKIKLNKIFSRITGKPLEQIELDTDRDNFMNPWEAKEYGLIDEVID 268
           I+ RE+ YHK +LN   +  TG+P+E+I  DT+RD FM+P EAK+YGLID+VID
Sbjct: 138 IQAREILYHKRRLNDYLAEHTGQPIERIAEDTERDFFMSPDEAKDYGLIDQVID 190

BLAST of MS019113 vs. ExPASy Swiss-Prot
Match: Q3MFR5 (ATP-dependent Clp protease proteolytic subunit 1 OS=Trichormus variabilis (strain ATCC 29413 / PCC 7937) OX=240292 GN=clpP1 PE=3 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 6.2e-55
Identity = 109/174 (62.64%), Postives = 138/174 (79.31%), Query Frame = 0

Query: 94  DTTNMLLRQRIIFLGSQVVDDMTADFIISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGI 153
           D  + LLR+RIIFLG Q VD   A+ I++QLLFLDAED  KDI L+INSPGGSVTAGMGI
Sbjct: 18  DIYSRLLRERIIFLGQQ-VDSNLANLIVAQLLFLDAEDPEKDIYLYINSPGGSVTAGMGI 77

Query: 154 YDAMKLCKADVSTVCLGLAASMGAFLLAAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMS 213
           +D MK  + DV T+C GLAASMGAFLL+AG+KGKR  +P+ R+MIHQPLG A G+AT++ 
Sbjct: 78  FDTMKHIRPDVCTICTGLAASMGAFLLSAGTKGKRMSLPHSRIMIHQPLGGAQGQATDIE 137

Query: 214 IRIREMAYHKIKLNKIFSRITGKPLEQIELDTDRDNFMNPWEAKEYGLIDEVID 268
           I+ RE+ YHK +LN   +  TG+P+E+I  DT+RD FM+P EA++YGLID+VID
Sbjct: 138 IQAREILYHKRRLNDYLAEHTGQPIERIAEDTERDFFMSPDEARDYGLIDQVID 190

BLAST of MS019113 vs. ExPASy Swiss-Prot
Match: O34125 (ATP-dependent Clp protease proteolytic subunit 2 OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=1140 GN=clpP2 PE=3 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 1.4e-54
Identity = 106/173 (61.27%), Postives = 138/173 (79.77%), Query Frame = 0

Query: 94  DTTNMLLRQRIIFLGSQVVDDMTADFIISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGI 153
           D  + LLR+RI+FLG+  VDD  AD I++QLLFL+AED  KDI+L+INSPGGSVTAGM I
Sbjct: 53  DIYSRLLRERIVFLGTG-VDDAVADSIVAQLLFLEAEDPEKDIQLYINSPGGSVTAGMAI 112

Query: 154 YDAMKLCKADVSTVCLGLAASMGAFLLAAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMS 213
           YD M+    DV+T+C GLAASMGAFLL+ G++GKR  +P+ R+MIHQPLG A G+A ++ 
Sbjct: 113 YDTMQQVAPDVATICFGLAASMGAFLLSGGAQGKRMALPSARIMIHQPLGGAQGQAVDIE 172

Query: 214 IRIREMAYHKIKLNKIFSRITGKPLEQIELDTDRDNFMNPWEAKEYGLIDEVI 267
           I+ RE+ YHK  LN + ++ TG+PLE+IE+DTDRD FM+P EAK YGLID+V+
Sbjct: 173 IQAREILYHKSTLNDLLAQHTGQPLEKIEVDTDRDFFMSPEEAKAYGLIDQVL 224

BLAST of MS019113 vs. ExPASy Swiss-Prot
Match: Q5N1P6 (ATP-dependent Clp protease proteolytic subunit 3 OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) OX=269084 GN=clpP3 PE=3 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 1.4e-54
Identity = 106/173 (61.27%), Postives = 138/173 (79.77%), Query Frame = 0

Query: 94  DTTNMLLRQRIIFLGSQVVDDMTADFIISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGI 153
           D  + LLR+RI+FLG+  VDD  AD I++QLLFL+AED  KDI+L+INSPGGSVTAGM I
Sbjct: 53  DIYSRLLRERIVFLGTG-VDDAVADSIVAQLLFLEAEDPEKDIQLYINSPGGSVTAGMAI 112

Query: 154 YDAMKLCKADVSTVCLGLAASMGAFLLAAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMS 213
           YD M+    DV+T+C GLAASMGAFLL+ G++GKR  +P+ R+MIHQPLG A G+A ++ 
Sbjct: 113 YDTMQQVAPDVATICFGLAASMGAFLLSGGAQGKRMALPSARIMIHQPLGGAQGQAVDIE 172

Query: 214 IRIREMAYHKIKLNKIFSRITGKPLEQIELDTDRDNFMNPWEAKEYGLIDEVI 267
           I+ RE+ YHK  LN + ++ TG+PLE+IE+DTDRD FM+P EAK YGLID+V+
Sbjct: 173 IQAREILYHKSTLNDLLAQHTGQPLEKIEVDTDRDFFMSPEEAKAYGLIDQVL 224

BLAST of MS019113 vs. ExPASy TrEMBL
Match: A0A6J1CQN6 (ATP-dependent Clp protease proteolytic subunit OS=Momordica charantia OX=3673 GN=LOC111013299 PE=3 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 1.0e-185
Identity = 336/337 (99.70%), Postives = 336/337 (99.70%), Query Frame = 0

Query: 1   MEMSFTYTPSPSPQRLMRFDHGVLSSQFPLFTPRKHSHGGNSSLNGLRTPKSAPLVKASL 60
           MEMSFTYTPSPSPQRLMRFDHGVLSSQFPLFTPRKHSHGGNSSLNGLRTPKSAPLVKASL
Sbjct: 1   MEMSFTYTPSPSPQRLMRFDHGVLSSQFPLFTPRKHSHGGNSSLNGLRTPKSAPLVKASL 60

Query: 61  SSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQVVDDMTADFI 120
           SSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQ VDDMTADFI
Sbjct: 61  SSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQ-VDDMTADFI 120

Query: 121 ISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAFLL 180
           ISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAFLL
Sbjct: 121 ISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAFLL 180

Query: 181 AAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPLEQ 240
           AAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPLEQ
Sbjct: 181 AAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPLEQ 240

Query: 241 IELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSRKA 300
           IELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSRKA
Sbjct: 241 IELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSRKA 300

Query: 301 KKNLPSEDKILQNGYQGGQGNDEERGTQQETEEPSPV 338
           KKNLPSEDKILQNGYQGGQGNDEERGTQQETEEPSPV
Sbjct: 301 KKNLPSEDKILQNGYQGGQGNDEERGTQQETEEPSPV 336

BLAST of MS019113 vs. ExPASy TrEMBL
Match: A0A1S3C615 (ATP-dependent Clp protease proteolytic subunit OS=Cucumis melo OX=3656 GN=LOC103496858 PE=3 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 1.7e-156
Identity = 291/340 (85.59%), Postives = 309/340 (90.88%), Query Frame = 0

Query: 1   MEMSFTYTPSPSPQRLMRFDHGV-LSSQFPLFTPRKHSHGGNSSLNGLRTPK-SAPLVK- 60
           ME+SFT+ PSPSP  L RFDHG+  SS  P+ TP  HSHG N + N  R    S P++  
Sbjct: 1   MELSFTHAPSPSPYGLKRFDHGLHHSSHVPISTPSNHSHGRNFTSNNFRIRNCSVPVLNH 60

Query: 61  ASLSSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQVVDDMTA 120
            SLSSSS+RRTLSSNWDVL N+SA+SAPSL RFEELDTTNMLLRQRIIFLGSQ VDD+TA
Sbjct: 61  PSLSSSSLRRTLSSNWDVLSNYSASSAPSLPRFEELDTTNMLLRQRIIFLGSQ-VDDVTA 120

Query: 121 DFIISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGA 180
           DFIISQLLFLDAED TKDIKLFINSPGGSVTAGMGIYDAMKLCKADVST+CLGLAASMGA
Sbjct: 121 DFIISQLLFLDAEDPTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTICLGLAASMGA 180

Query: 181 FLLAAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKP 240
           FLLA+GSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKP
Sbjct: 181 FLLASGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKP 240

Query: 241 LEQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGS 300
           LEQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIA+A  PPKTRVWDLWKVEGS
Sbjct: 241 LEQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIAEATPPPKTRVWDLWKVEGS 300

Query: 301 RKAKKNLPSEDKILQNGYQGGQGNDEERGTQQETEEPSPV 338
           RKAKKNLPSE KIL+NGYQGG+G+DEERG+QQETEEPSPV
Sbjct: 301 RKAKKNLPSESKILKNGYQGGEGSDEERGSQQETEEPSPV 339

BLAST of MS019113 vs. ExPASy TrEMBL
Match: A0A0A0LA84 (ATP-dependent Clp protease proteolytic subunit OS=Cucumis sativus OX=3659 GN=Csa_3G363170 PE=3 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 2.7e-154
Identity = 288/340 (84.71%), Postives = 305/340 (89.71%), Query Frame = 0

Query: 1   MEMSFTYTPSPSPQRLMRFDHGVLSSQFPLFTPRKHSHGGNSSLNGLRTPK-SAPLVK-A 60
           ME+SFT+ PSPSP  L R DHG  SS FP+ TP KHSHGGN + N  R    S P+V   
Sbjct: 1   MELSFTHAPSPSPYGLKRSDHGFQSSHFPISTPSKHSHGGNFTSNAFRIRNCSVPVVNHP 60

Query: 61  SLSSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQVVDDMTAD 120
           S SSSS+RRTLSSNWDV  N+S +SA SL RFEELDTTNMLLRQRIIFLGSQ VDDMTAD
Sbjct: 61  SHSSSSLRRTLSSNWDVFSNYSVSSARSLPRFEELDTTNMLLRQRIIFLGSQ-VDDMTAD 120

Query: 121 FIISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAF 180
           FIISQLLFLDAED TKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAF
Sbjct: 121 FIISQLLFLDAEDPTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAF 180

Query: 181 LLAAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPL 240
           LLA+GSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKP+
Sbjct: 181 LLASGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPI 240

Query: 241 EQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSR 300
           EQIELDT+RDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIA+A  PPKTR+WDLWKVEGSR
Sbjct: 241 EQIELDTERDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIAEATPPPKTRIWDLWKVEGSR 300

Query: 301 KAKKNLPSEDKILQNGY-QGGQGNDEERGTQQETEEPSPV 338
           KAKKNLPSE KIL+NGY +GGQG+D+ERG QQETEEPSPV
Sbjct: 301 KAKKNLPSESKILKNGYEEGGQGSDKERGGQQETEEPSPV 339

BLAST of MS019113 vs. ExPASy TrEMBL
Match: A0A6J1G9P1 (ATP-dependent Clp protease proteolytic subunit OS=Cucurbita moschata OX=3662 GN=LOC111452225 PE=3 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 2.7e-154
Identity = 290/339 (85.55%), Postives = 305/339 (89.97%), Query Frame = 0

Query: 1   MEMSFTYTPSPS-PQRLMRFDHGVLSSQFPLFTPRKHSHGGNSSLNGLRTPKSAPL--VK 60
           ME+SFTY PSPS   RLM F+HGVL+S FP+ TP KHSHG NSS N  R  KS P+  VK
Sbjct: 1   MELSFTYAPSPSLHSRLMCFNHGVLASPFPVSTPFKHSHGANSSSNAFRIRKSVPVAAVK 60

Query: 61  ASLSSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQVVDDMTA 120
           ASLSSS +RRTLSSNWDVL N+SA SAP+L RFEELDTTNMLLRQRIIFLGSQ VDDMTA
Sbjct: 61  ASLSSSPLRRTLSSNWDVLGNYSAASAPALPRFEELDTTNMLLRQRIIFLGSQ-VDDMTA 120

Query: 121 DFIISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGA 180
           DFIISQLLFLDAED TKDIKL INSPGGSVTAGMGIYDAMKLCKADVSTVC+GLAASMGA
Sbjct: 121 DFIISQLLFLDAEDPTKDIKLLINSPGGSVTAGMGIYDAMKLCKADVSTVCMGLAASMGA 180

Query: 181 FLLAAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKP 240
           FLLAAGSKGKRFCMPNGRVMIHQPLGTAGG+AT+MSIR+REMAYHKIKLNKI SRITGKP
Sbjct: 181 FLLAAGSKGKRFCMPNGRVMIHQPLGTAGGRATDMSIRLREMAYHKIKLNKILSRITGKP 240

Query: 241 LEQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGS 300
           LEQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIA+A  PPKTRVWDLWK+EGS
Sbjct: 241 LEQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIAEANPPPKTRVWDLWKIEGS 300

Query: 301 RKAKKNLPSEDKILQNGYQGGQGNDEERGTQQETEEPSP 337
           RKAKKNLPSE KILQNGY   QG+DEER  Q ETEEPSP
Sbjct: 301 RKAKKNLPSETKILQNGY---QGSDEERSPQPETEEPSP 335

BLAST of MS019113 vs. ExPASy TrEMBL
Match: A0A5A7UZN2 (ATP-dependent Clp protease proteolytic subunit OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G00040 PE=3 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 1.9e-152
Identity = 291/375 (77.60%), Postives = 309/375 (82.40%), Query Frame = 0

Query: 1   MEMSFTYTPSPSPQRLMRFDHGV-LSSQFPLFTPRKHSHGGNSSLNGLRTPK-SAPLVK- 60
           ME+SFT+ PSPSP  L RFDHG+  SS  P+ TP  HSHG N + N  R    S P++  
Sbjct: 1   MELSFTHAPSPSPYGLKRFDHGLHHSSHVPISTPSNHSHGRNFTSNNFRIRNCSVPVLNH 60

Query: 61  ASLSSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQV------ 120
            SLSSSS+RRTLSSNWDVL N+SA+SAPSL RFEELDTTNMLLRQRIIFLGSQ       
Sbjct: 61  PSLSSSSLRRTLSSNWDVLSNYSASSAPSLPRFEELDTTNMLLRQRIIFLGSQAMSPTIS 120

Query: 121 -----------------------------VDDMTADFIISQLLFLDAEDSTKDIKLFINS 180
                                        VDD+TADFIISQLLFLDAED TKDIKLFINS
Sbjct: 121 SYFLITCCLSWKKKRGFLLGLEYCIGYWQVDDVTADFIISQLLFLDAEDPTKDIKLFINS 180

Query: 181 PGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAFLLAAGSKGKRFCMPNGRVMIHQPL 240
           PGGSVTAGMGIYDAMKLCKADVST+CLGLAASMGAFLLA+GSKGKRFCMPNGRVMIHQPL
Sbjct: 181 PGGSVTAGMGIYDAMKLCKADVSTICLGLAASMGAFLLASGSKGKRFCMPNGRVMIHQPL 240

Query: 241 GTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPLEQIELDTDRDNFMNPWEAKEYGLI 300
           GTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPLEQIELDTDRDNFMNPWEAKEYGLI
Sbjct: 241 GTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPLEQIELDTDRDNFMNPWEAKEYGLI 300

Query: 301 DEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSRKAKKNLPSEDKILQNGYQGGQGND 338
           DEVIDDGKPGLIAPIA+A  PPKTRVWDLWKVEGSRKAKKNLPSE KIL+NGYQGG+G+D
Sbjct: 301 DEVIDDGKPGLIAPIAEATPPPKTRVWDLWKVEGSRKAKKNLPSESKILKNGYQGGEGSD 360

BLAST of MS019113 vs. TAIR 10
Match: AT1G66670.1 (CLP protease proteolytic subunit 3 )

HSP 1 Score: 405.2 bits (1040), Expect = 5.1e-113
Identity = 218/306 (71.24%), Postives = 242/306 (79.08%), Query Frame = 0

Query: 17  MRFDHGVLSSQFPLFTPRKHSHGGNSSLNGLRTPKSAP--LVKASLS-SSSMRRTLSSNW 76
           +R      S+   L  P K+    N  +   R PK++    V++S+S S   R+TLSSNW
Sbjct: 5   LRLASSSTSNPICLLNPGKNL---NFPIRNHRIPKTSKPFCVRSSMSLSKPPRQTLSSNW 64

Query: 77  DVLENFS----ATSAPSLARFEELDTTNMLLRQRIIFLGSQVVDDMTADFIISQLLFLDA 136
           DV  +FS    A S   L  FEELDTTNMLLRQRI+FLGSQ VDDMTAD +ISQLL LDA
Sbjct: 65  DV-SSFSIDSVAQSPSRLPSFEELDTTNMLLRQRIVFLGSQ-VDDMTADLVISQLLLLDA 124

Query: 137 EDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAFLLAAGSKGKRF 196
           EDS +DI LFINSPGGS+TAGMGIYDAMK CKADVSTVCLGLAASMGAFLLA+GSKGKR+
Sbjct: 125 EDSERDITLFINSPGGSITAGMGIYDAMKQCKADVSTVCLGLAASMGAFLLASGSKGKRY 184

Query: 197 CMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPLEQIELDTDRDN 256
           CMPN +VMIHQPLGTAGGKATEMSIRIREM YHKIKLNKIFSRITGKP  +IE DTDRDN
Sbjct: 185 CMPNSKVMIHQPLGTAGGKATEMSIRIREMMYHKIKLNKIFSRITGKPESEIESDTDRDN 244

Query: 257 FMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSRKAKKNLPSEDK 316
           F+NPWEAKEYGLID VIDDGKPGLIAPI D   PPKT+VWDLWKVEG++K   NLPSE  
Sbjct: 245 FLNPWEAKEYGLIDAVIDDGKPGLIAPIGDGTPPPKTKVWDLWKVEGTKKDNTNLPSERS 304

BLAST of MS019113 vs. TAIR 10
Match: AT5G45390.1 (CLP protease P4 )

HSP 1 Score: 197.6 bits (501), Expect = 1.6e-50
Identity = 115/261 (44.06%), Postives = 158/261 (60.54%), Query Frame = 0

Query: 25  SSQFPLFTPRKHSHGGNSSLNGLRTPKSAPLVKASLSSSSMRRTLSS--NWDVLENFSAT 84
           SS  P     + +   ++S +    P +  L    L S  +R T  S   +       + 
Sbjct: 8   SSLKPSLVSSRLNSSSSASSSSFPKPNNLYLKPTKLISPPLRTTSPSPLRFANASIEMSQ 67

Query: 85  SAPSLARFEELDTTNMLLRQRIIFLGSQVVDDMTADFIISQLLFLDAEDSTKDIKLFINS 144
           +  S  R  E D   +LLR+RI+FLGS  +DD  AD I+SQLL LDA+D  KDIKLFINS
Sbjct: 68  TQESAIRGAESDVMGLLLRERIVFLGSS-IDDFVADAIMSQLLLLDAKDPKKDIKLFINS 127

Query: 145 PGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAFLLAAGSKGKRFCMPNGRVMIHQPL 204
           PGGS++A M IYD ++L +ADVST+ LG+AAS  + +L AG+KGKRF MPN R+MIHQPL
Sbjct: 128 PGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGAGTKGKRFAMPNTRIMIHQPL 187

Query: 205 GTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPLEQIELDTDRDNFMNPWEAKEYGLI 264
           G A G+A ++ I+ +E+ ++K  +  I +  T +  EQ+  D DRD +M+P EA EYGLI
Sbjct: 188 GGASGQAIDVEIQAKEVMHNKNNVTSIIAGCTSRSFEQVLKDIDRDRYMSPIEAVEYGLI 247

Query: 265 DEVIDDGKPGLIAPIADAAAP 284
           D VID      + P+ D   P
Sbjct: 248 DGVIDGDSIIPLEPVPDRVKP 267

BLAST of MS019113 vs. TAIR 10
Match: AT1G02560.1 (nuclear encoded CLP protease 5 )

HSP 1 Score: 197.2 bits (500), Expect = 2.1e-50
Identity = 104/178 (58.43%), Postives = 132/178 (74.16%), Query Frame = 0

Query: 89  RFEELDTTNMLLRQRIIFLGSQVVDDMTADFIISQLLFLDAEDSTKDIKLFINSPGGSVT 148
           RF+ +   + L + RII  G  V DDM A+ I++QLL+LDA D TKDI +++NSPGGSVT
Sbjct: 111 RFQSI--ISQLFQYRIIRCGGAVDDDM-ANIIVAQLLYLDAVDPTKDIVMYVNSPGGSVT 170

Query: 149 AGMGIYDAMKLCKADVSTVCLGLAASMGAFLLAAGSKGKRFCMPNGRVMIHQPLGTAGGK 208
           AGM I+D M+  + DVSTVC+GLAASMGAFLL+AG+KGKR+ +PN R+MIHQPLG A G 
Sbjct: 171 AGMAIFDTMRHIRPDVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGG 230

Query: 209 ATEMSIRIREMAYHKIKLNKIFSRITGKPLEQIELDTDRDNFMNPWEAKEYGLIDEVI 267
            T++ I+  EM +HK  LN   +  TG+ LE+I  DTDRD FM+  EAKEYGLID VI
Sbjct: 231 QTDIDIQANEMLHHKANLNGYLAYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVI 285

BLAST of MS019113 vs. TAIR 10
Match: AT5G23140.1 (nuclear-encoded CLP protease P7 )

HSP 1 Score: 164.9 bits (416), Expect = 1.2e-40
Identity = 80/175 (45.71%), Postives = 126/175 (72.00%), Query Frame = 0

Query: 94  DTTNMLLRQRIIFLGSQVVDDMTADFIISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGI 153
           D  + LL++RII +   + DD T+  +++QLL+L++E+ +K I +++NSPGG VTAG+ I
Sbjct: 48  DIFSRLLKERIICINGPINDD-TSHVVVAQLLYLESENPSKPIHMYLNSPGGHVTAGLAI 107

Query: 154 YDAMKLCKADVSTVCLGLAASMGAFLLAAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMS 213
           YD M+  ++ +ST+CLG AASM + LLAAG+KG+R  +PN  VMIHQP G   G+A +++
Sbjct: 108 YDTMQYIRSPISTICLGQAASMASLLLAAGAKGQRRSLPNATVMIHQPSGGYSGQAKDIT 167

Query: 214 IRIREMAYHKIKLNKIFSRITGKPLEQIELDTDRDNFMNPWEAKEYGLIDEVIDD 269
           I  +++      LN+++ + TG+PL+ +  + DRD+FM P EAK +G+IDEVID+
Sbjct: 168 IHTKQIVRVWDALNELYVKHTGQPLDVVANNMDRDHFMTPEEAKAFGIIDEVIDE 221

BLAST of MS019113 vs. TAIR 10
Match: AT1G11750.1 (CLP protease proteolytic subunit 6 )

HSP 1 Score: 146.0 bits (367), Expect = 5.6e-35
Identity = 75/175 (42.86%), Postives = 113/175 (64.57%), Query Frame = 0

Query: 93  LDTTNMLLRQRIIFLGSQVVDDMTADFIISQLLFLDAEDSTKDIKLFINSPGGSVTAGMG 152
           LD +++L R RIIF+G Q ++   A  +ISQL+ L + D   DI +++N PGGS  + + 
Sbjct: 95  LDLSSVLFRNRIIFIG-QPINAQVAQRVISQLVTLASIDDKSDILMYLNCPGGSTYSVLA 154

Query: 153 IYDAMKLCKADVSTVCLGLAASMGAFLLAAGSKGKRFCMPNGRVMIHQPLGTAGGKATEM 212
           IYD M   K  V TV  G+AAS GA LLA G KG R+ MPN RVMIHQP    GG   ++
Sbjct: 155 IYDCMSWIKPKVGTVAFGVAASQGALLLAGGEKGMRYAMPNTRVMIHQPQTGCGGHVEDV 214

Query: 213 SIRIREMAYHKIKLNKIFSRITGKPLEQIELDTDRDNFMNPWEAKEYGLIDEVID 268
             ++ E    + K++++++  TG+PLE+++  T+RD F++  EA E+GLID +++
Sbjct: 215 RRQVNEAIEARQKIDRMYAAFTGQPLEKVQQYTERDRFLSASEALEFGLIDGLLE 268

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022143418.12.1e-18599.70ATP-dependent Clp protease proteolytic subunit 3, chloroplastic [Momordica chara... [more]
XP_038902354.15.2e-16086.05ATP-dependent Clp protease proteolytic subunit 3, chloroplastic [Benincasa hispi... [more]
XP_008457103.13.5e-15685.59PREDICTED: ATP-dependent Clp protease proteolytic subunit 3, chloroplastic [Cucu... [more]
XP_022948597.15.6e-15485.55ATP-dependent Clp protease proteolytic subunit 3, chloroplastic [Cucurbita mosch... [more]
XP_011651427.15.6e-15484.71ATP-dependent Clp protease proteolytic subunit 3, chloroplastic [Cucumis sativus... [more]
Match NameE-valueIdentityDescription
Q9SXJ67.2e-11271.24ATP-dependent Clp protease proteolytic subunit 3, chloroplastic OS=Arabidopsis t... [more]
Q8YXH53.6e-5562.64ATP-dependent Clp protease proteolytic subunit 1 OS=Nostoc sp. (strain PCC 7120 ... [more]
Q3MFR56.2e-5562.64ATP-dependent Clp protease proteolytic subunit 1 OS=Trichormus variabilis (strai... [more]
O341251.4e-5461.27ATP-dependent Clp protease proteolytic subunit 2 OS=Synechococcus elongatus (str... [more]
Q5N1P61.4e-5461.27ATP-dependent Clp protease proteolytic subunit 3 OS=Synechococcus sp. (strain AT... [more]
Match NameE-valueIdentityDescription
A0A6J1CQN61.0e-18599.70ATP-dependent Clp protease proteolytic subunit OS=Momordica charantia OX=3673 GN... [more]
A0A1S3C6151.7e-15685.59ATP-dependent Clp protease proteolytic subunit OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A0A0LA842.7e-15484.71ATP-dependent Clp protease proteolytic subunit OS=Cucumis sativus OX=3659 GN=Csa... [more]
A0A6J1G9P12.7e-15485.55ATP-dependent Clp protease proteolytic subunit OS=Cucurbita moschata OX=3662 GN=... [more]
A0A5A7UZN21.9e-15277.60ATP-dependent Clp protease proteolytic subunit OS=Cucumis melo var. makuwa OX=11... [more]
Match NameE-valueIdentityDescription
AT1G66670.15.1e-11371.24CLP protease proteolytic subunit 3 [more]
AT5G45390.11.6e-5044.06CLP protease P4 [more]
AT1G02560.12.1e-5058.43nuclear encoded CLP protease 5 [more]
AT5G23140.11.2e-4045.71nuclear-encoded CLP protease P7 [more]
AT1G11750.15.6e-3542.86CLP protease proteolytic subunit 6 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001907ATP-dependent Clp protease proteolytic subunitPRINTSPR00127CLPPROTEASEPcoord: 187..206
score: 52.0
coord: 244..263
score: 58.0
coord: 166..183
score: 68.61
coord: 94..109
score: 46.25
coord: 135..155
score: 64.64
IPR001907ATP-dependent Clp protease proteolytic subunitHAMAPMF_00444ClpPcoord: 75..269
score: 36.716984
IPR001907ATP-dependent Clp protease proteolytic subunitCDDcd07017S14_ClpP_2coord: 94..265
e-value: 3.06324E-101
score: 293.579
IPR023562Clp protease proteolytic subunit /Translocation-enhancing protein TepAPFAMPF00574CLP_proteasecoord: 93..267
e-value: 1.9E-72
score: 243.0
IPR023562Clp protease proteolytic subunit /Translocation-enhancing protein TepAPANTHERPTHR10381ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNITcoord: 66..313
NoneNo IPR availableGENE3D3.90.226.10coord: 79..270
e-value: 4.2E-70
score: 237.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 297..337
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 309..324
NoneNo IPR availablePANTHERPTHR10381:SF50ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT 3, CHLOROPLASTICcoord: 66..313
IPR018215ClpP, Ser active sitePROSITEPS00381CLP_PROTEASE_SERcoord: 166..177
IPR033135ClpP, histidine active sitePROSITEPS00382CLP_PROTEASE_HIScoord: 188..201
IPR029045ClpP/crotonase-like domain superfamilySUPERFAMILY52096ClpP/crotonasecoord: 93..268

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS019113.1MS019113.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004176 ATP-dependent peptidase activity
molecular_function GO:0004252 serine-type endopeptidase activity