MS019021 (gene) Bitter gourd (TR) v1

BLAST of MS019021 vs. NCBI nr
Match: XP_022155314.1 (pectinesterase inhibitor-like [Momordica charantia])

HSP 1 Score: 330.1 bits (845), Expect = 1.1e-86
Identity = 168/168 (100.00%), Postives = 168/168 (100.00%), Query Frame = 0

Query: 1   LSVAAAAEAIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPADKTGLALVALRLVS 60
           LSVAAAAEAIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPADKTGLALVALRLVS
Sbjct: 15  LSVAAAAEAIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPADKTGLALVALRLVS 74

Query: 61  SNASDISEGIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDSIAALLVKAYGDVDK 120
           SNASDISEGIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDSIAALLVKAYGDVDK
Sbjct: 75  SNASDISEGIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDSIAALLVKAYGDVDK 134

Query: 121 WVNAAVAAVRTCEASFSVKPSVLTPRNDNFIKLCQNALTIAHIAETEN 169
           WVNAAVAAVRTCEASFSVKPSVLTPRNDNFIKLCQNALTIAHIAETEN
Sbjct: 135 WVNAAVAAVRTCEASFSVKPSVLTPRNDNFIKLCQNALTIAHIAETEN 182

BLAST of MS019021 vs. NCBI nr
Match: XP_038906317.1 (cell wall / vacuolar inhibitor of fructosidase 2-like [Benincasa hispida])

HSP 1 Score: 218.4 bits (555), Expect = 4.7e-53
Identity = 109/164 (66.46%), Postives = 134/164 (81.71%), Query Frame = 0

Query: 3   VAAAAEAIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPADKTGLALVALRLVSSN 62
           VA   +A  P +  + +I+  C+QT +  LC+ SL SDPNSFPADK GLALVALR  SSN
Sbjct: 16  VAQRLDAASPAEDGLSMIDHMCAQTNYKDLCVISLTSDPNSFPADKMGLALVALRFASSN 75

Query: 63  ASDISEGIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDSIAALLVKAYGDVDKWV 122
           ASDISE IKVMLNDT L ++P VQQGL+DCLDEYLDASQQLDD+IAA+LVKAYGDV+KWV
Sbjct: 76  ASDISESIKVMLNDTSL-NKPSVQQGLFDCLDEYLDASQQLDDAIAAILVKAYGDVEKWV 135

Query: 123 NAAVAAVRTCEASFSVKPSVLTPRNDNFIKLCQNALTIAHIAET 167
           +AA+A+VRTCE  F+ KPS+L P+ND FIKLC+ AL+I+ +A+T
Sbjct: 136 DAAIASVRTCENEFATKPSLLAPKNDVFIKLCEIALSISKVADT 178

BLAST of MS019021 vs. NCBI nr
Match: KAA0044390.1 (putative invertase inhibitor [Cucumis melo var. makuwa] >TYK29518.1 putative invertase inhibitor [Cucumis melo var. makuwa])

HSP 1 Score: 215.7 bits (548), Expect = 3.1e-52
Identity = 111/157 (70.70%), Postives = 129/157 (82.17%), Query Frame = 0

Query: 9   AIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPADKTGLALVALRLVSSNASDISE 68
           A +  K  +  IE+ C+QT +  LC++SL SDPNSFPADK GLALVALRL SSNASDISE
Sbjct: 9   ASQANKEGIEKIEKMCAQTNYKDLCVTSLTSDPNSFPADKMGLALVALRLASSNASDISE 68

Query: 69  GIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDSIAALLVKAYGDVDKWVNAAVAA 128
            IKVMLN+T   ++P VQQGL+DCLDEYLDASQQLDDSIAA++ KAYGDV+KWV+AAVA 
Sbjct: 69  SIKVMLNETS-QNEPAVQQGLFDCLDEYLDASQQLDDSIAAIIAKAYGDVEKWVHAAVAD 128

Query: 129 VRTCEASFSVKPSVLTPRNDNFIKLCQNALTIAHIAE 166
           VRTCE SF  KPSVLTPRN+ FIKLC  AL+I+ IAE
Sbjct: 129 VRTCENSFPTKPSVLTPRNEEFIKLCDIALSISRIAE 164

BLAST of MS019021 vs. NCBI nr
Match: XP_004152360.2 (cell wall / vacuolar inhibitor of fructosidase 2 [Cucumis sativus] >KGN52826.1 hypothetical protein Csa_015223 [Cucumis sativus])

HSP 1 Score: 213.0 bits (541), Expect = 2.0e-51
Identity = 109/166 (65.66%), Postives = 130/166 (78.31%), Query Frame = 0

Query: 1   LSVAAAAEAIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPADKTGLALVALRLVS 60
           L  A AA+     +  +G+I++ C+QT +  LC++SL SDPNSFPADK GLALVALRL S
Sbjct: 20  LDAAGAAQ-----EEGLGMIQKMCAQTNYKDLCITSLTSDPNSFPADKMGLALVALRLAS 79

Query: 61  SNASDISEGIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDSIAALLVKAYGDVDK 120
           SNASDISE IKVMLN+T   ++P VQQ L+DCLDEYL+ASQQLDDSIAA++ KAYGDV +
Sbjct: 80  SNASDISESIKVMLNETSQNNEPTVQQALFDCLDEYLEASQQLDDSIAAIIAKAYGDVQE 139

Query: 121 WVNAAVAAVRTCEASFSVKPSVLTPRNDNFIKLCQNALTIAHIAET 167
           WV  AV  VRTCE+SF  KPSVLTPRN+ FIKLC  AL+I  IAET
Sbjct: 140 WVRVAVTNVRTCESSFPTKPSVLTPRNEEFIKLCDIALSITKIAET 180

BLAST of MS019021 vs. NCBI nr
Match: XP_023528768.1 (pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 204.9 bits (520), Expect = 5.4e-49
Identity = 109/161 (67.70%), Postives = 126/161 (78.26%), Query Frame = 0

Query: 8   EAIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPADKTGLALVALRLVSSNASDIS 67
           +A RP +  M LIE+ CSQT+H  LC+ SL SDPNS+PADKTGLALVALR  SSNASDIS
Sbjct: 23  DATRPVEEGMALIEEMCSQTKHKELCVDSLTSDPNSYPADKTGLALVALRFASSNASDIS 82

Query: 68  EGIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDSIAALLVKAYGDVDKWVNAAVA 127
           E IKVM ND+ LA +P VQ+ L DCLDEYLDASQQLDDSIAAL VKAYGD++KWVN A+ 
Sbjct: 83  ESIKVMFNDSTLA-KPMVQEALLDCLDEYLDASQQLDDSIAALTVKAYGDLEKWVNIAMT 142

Query: 128 AVRTCEASF-SVKPSVLTPRNDNFIKLCQNALTIAHIAETE 168
            VRTC     S +P +LT RND FIKLC+ ALTI+ IA+ E
Sbjct: 143 DVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIAQGE 182

BLAST of MS019021 vs. ExPASy Swiss-Prot
Match: Q0J8J8 (Pectinesterase inhibitor 28 OS=Oryza sativa subsp. japonica OX=39947 GN=PMEI28 PE=2 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 6.3e-08
Identity = 44/152 (28.95%), Postives = 71/152 (46.71%), Query Frame = 0

Query: 19  LIEQTCSQTQHPGLCLSSLKSDPNSFPADKTGLALVALRLVSSNASDISEGIKVMLNDT- 78
           L+  TC+ T +  +C+S+L +DP+S  AD  GL+ +A+ + ++NAS  ++    + N T 
Sbjct: 61  LVRATCNSTAYYDVCVSALAADPSSTTADVRGLSAIAVSVAAANASGAAQAAAALANGTA 120

Query: 79  ----RLADQPGVQQGLYDCLDEYLDASQQLDDSIAALLVKAYGDVDKWVNAAVAAVRTCE 138
                 A    VQ  L  C  +Y DA   L  +  ++  + Y      V+A     + C+
Sbjct: 121 PLAAAAAGDGTVQALLRACAGKYGDARDALAAAKESMGQQDYDLATVHVSAGAEYPQVCK 180

Query: 139 ASF-----SVKPSVLTPRNDNFIKLCQNALTI 161
           A F        P+ L  R +   KLC  AL I
Sbjct: 181 ALFRRQRPGAYPAELAAREEALNKLCSVALDI 212

BLAST of MS019021 vs. ExPASy TrEMBL
Match: A0A6J1DPW6 (pectinesterase inhibitor-like OS=Momordica charantia OX=3673 GN=LOC111022446 PE=4 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 5.4e-87
Identity = 168/168 (100.00%), Postives = 168/168 (100.00%), Query Frame = 0

Query: 1   LSVAAAAEAIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPADKTGLALVALRLVS 60
           LSVAAAAEAIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPADKTGLALVALRLVS
Sbjct: 15  LSVAAAAEAIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPADKTGLALVALRLVS 74

Query: 61  SNASDISEGIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDSIAALLVKAYGDVDK 120
           SNASDISEGIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDSIAALLVKAYGDVDK
Sbjct: 75  SNASDISEGIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDSIAALLVKAYGDVDK 134

Query: 121 WVNAAVAAVRTCEASFSVKPSVLTPRNDNFIKLCQNALTIAHIAETEN 169
           WVNAAVAAVRTCEASFSVKPSVLTPRNDNFIKLCQNALTIAHIAETEN
Sbjct: 135 WVNAAVAAVRTCEASFSVKPSVLTPRNDNFIKLCQNALTIAHIAETEN 182

BLAST of MS019021 vs. ExPASy TrEMBL
Match: A0A5D3E196 (Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001210 PE=4 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 1.5e-52
Identity = 111/157 (70.70%), Postives = 129/157 (82.17%), Query Frame = 0

Query: 9   AIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPADKTGLALVALRLVSSNASDISE 68
           A +  K  +  IE+ C+QT +  LC++SL SDPNSFPADK GLALVALRL SSNASDISE
Sbjct: 9   ASQANKEGIEKIEKMCAQTNYKDLCVTSLTSDPNSFPADKMGLALVALRLASSNASDISE 68

Query: 69  GIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDSIAALLVKAYGDVDKWVNAAVAA 128
            IKVMLN+T   ++P VQQGL+DCLDEYLDASQQLDDSIAA++ KAYGDV+KWV+AAVA 
Sbjct: 69  SIKVMLNETS-QNEPAVQQGLFDCLDEYLDASQQLDDSIAAIIAKAYGDVEKWVHAAVAD 128

Query: 129 VRTCEASFSVKPSVLTPRNDNFIKLCQNALTIAHIAE 166
           VRTCE SF  KPSVLTPRN+ FIKLC  AL+I+ IAE
Sbjct: 129 VRTCENSFPTKPSVLTPRNEEFIKLCDIALSISRIAE 164

BLAST of MS019021 vs. ExPASy TrEMBL
Match: A0A0A0KY96 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G001930 PE=4 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 9.6e-52
Identity = 109/166 (65.66%), Postives = 130/166 (78.31%), Query Frame = 0

Query: 1   LSVAAAAEAIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPADKTGLALVALRLVS 60
           L  A AA+     +  +G+I++ C+QT +  LC++SL SDPNSFPADK GLALVALRL S
Sbjct: 20  LDAAGAAQ-----EEGLGMIQKMCAQTNYKDLCITSLTSDPNSFPADKMGLALVALRLAS 79

Query: 61  SNASDISEGIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDSIAALLVKAYGDVDK 120
           SNASDISE IKVMLN+T   ++P VQQ L+DCLDEYL+ASQQLDDSIAA++ KAYGDV +
Sbjct: 80  SNASDISESIKVMLNETSQNNEPTVQQALFDCLDEYLEASQQLDDSIAAIIAKAYGDVQE 139

Query: 121 WVNAAVAAVRTCEASFSVKPSVLTPRNDNFIKLCQNALTIAHIAET 167
           WV  AV  VRTCE+SF  KPSVLTPRN+ FIKLC  AL+I  IAET
Sbjct: 140 WVRVAVTNVRTCESSFPTKPSVLTPRNEEFIKLCDIALSITKIAET 180

BLAST of MS019021 vs. ExPASy TrEMBL
Match: A0A6J1J9E7 (pectinesterase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111482494 PE=4 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 9.9e-49
Identity = 108/161 (67.08%), Postives = 126/161 (78.26%), Query Frame = 0

Query: 8   EAIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPADKTGLALVALRLVSSNASDIS 67
           +A RP +  M LIE+ CSQT+H  LC+ SL SDPNS+PADKTGLALVALR  SSNASDIS
Sbjct: 21  DATRPVEEGMTLIEEMCSQTKHKELCVDSLTSDPNSYPADKTGLALVALRFASSNASDIS 80

Query: 68  EGIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDSIAALLVKAYGDVDKWVNAAVA 127
           E IKVM ND+ L+ +P VQ+ L DCLDEYLDASQQLDDSIAAL VKAYGD++KWVN A+ 
Sbjct: 81  ESIKVMFNDSTLS-KPMVQEALLDCLDEYLDASQQLDDSIAALTVKAYGDLEKWVNIAMT 140

Query: 128 AVRTCEASF-SVKPSVLTPRNDNFIKLCQNALTIAHIAETE 168
            VRTC     S +P +LT RND FIKLC+ ALTI+ IA+ E
Sbjct: 141 DVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIAQGE 180

BLAST of MS019021 vs. ExPASy TrEMBL
Match: A0A6J1F7F2 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111441544 PE=4 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 9.9e-49
Identity = 107/161 (66.46%), Postives = 126/161 (78.26%), Query Frame = 0

Query: 8   EAIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPADKTGLALVALRLVSSNASDIS 67
           +A RP +  M LIE+ CSQT+H  LC+ SL SDPNS+PADKTGLALVALR  SSNASDIS
Sbjct: 21  DATRPVEEGMALIEEMCSQTKHKELCVDSLTSDPNSYPADKTGLALVALRFASSNASDIS 80

Query: 68  EGIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDSIAALLVKAYGDVDKWVNAAVA 127
           E IKVM ND+ L+ +P VQ+ L DCLDEYLDASQQLDDSIAAL VKAYGD++KWVN A+ 
Sbjct: 81  ESIKVMFNDSTLS-KPMVQEALLDCLDEYLDASQQLDDSIAALTVKAYGDLEKWVNIAIT 140

Query: 128 AVRTCEASF-SVKPSVLTPRNDNFIKLCQNALTIAHIAETE 168
            VRTC     S +P +LT RN+ FIKLC+ ALTI+ IA+ E
Sbjct: 141 DVRTCGTELGSERPPLLTERNEVFIKLCEIALTISKIAQGE 180

BLAST of MS019021 vs. TAIR 10
Match: AT1G55770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 107.8 bits (268), Expect = 8.4e-24
Identity = 59/153 (38.56%), Postives = 92/153 (60.13%), Query Frame = 0

Query: 13  GKPDMGLIEQTCSQTQHPGLCLSSLKSDPNS--FPADKTGLALVALRLVSSNASDISEGI 72
           G  + G+I   C  +  P LCLSS+ S P S  F      + ++A+   S+NAS  S  I
Sbjct: 20  GNANSGMISDLCKHSDDPNLCLSSITSRPESGEFAGTSNQIEIIAISAASANASATSSYI 79

Query: 73  KVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDSIAALLVKAYGDVDKWVNAAVAAVR 132
           K  L++  L  +P ++  L DC  +Y DA +QLDDSI+A+L  A+ DVD W++AA++A+ 
Sbjct: 80  KQKLSNEDL--EPAIEDTLEDCQKDYQDAVEQLDDSISAMLADAHTDVDVWLSAAISAIE 139

Query: 133 TCEASFSVK---PSVLTPRNDNFIKLCQNALTI 161
           +C ++   +    + L+ RN+ F+KLC+NAL I
Sbjct: 140 SCGSALGSRAGNDAELSQRNEVFLKLCKNALMI 170

BLAST of MS019021 vs. TAIR 10
Match: AT4G02250.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 91.7 bits (226), Expect = 6.2e-19
Identity = 54/131 (41.22%), Postives = 77/131 (58.78%), Query Frame = 0

Query: 33  CLSSLKSDPNSFPADKTGLALVALRLVSSNASDISEGIKVMLNDTRLADQPGVQQGLYDC 92
           C++SL S P +  A    L ++AL + SSNASD S  IK  L    L  +P ++  L DC
Sbjct: 26  CVASLTSRPEAATATAPKLGVIALSIASSNASDTSFYIKAKLKQKNL--EPALEDTLDDC 85

Query: 93  LDEYLDASQQLDDSIAALLVKAYGDVDKWVNAAVAAVRTCEASFSVK---PSVLTPRNDN 152
              YLDA  QLDDS+AAL+  ++ DVD W+N A++    CE + + +    + L  RN N
Sbjct: 86  SKNYLDAVAQLDDSLAALMQNSFIDVDIWLNTAISDGEACENALNDRAGNDAELARRNTN 145

Query: 153 FIKLCQNALTI 161
            +KLC++AL I
Sbjct: 146 LLKLCKDALLI 154

BLAST of MS019021 vs. TAIR 10
Match: AT5G46940.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 49.7 bits (117), Expect = 2.7e-06
Identity = 33/125 (26.40%), Postives = 64/125 (51.20%), Query Frame = 0

Query: 32  LCLSSLKSDPNSFPA-DKTGLALVALRLVSSNASDISEGIKVMLNDTRLADQPGVQQGLY 91
           LC++SL+++P +  A D  GL + + +   + A+ +   +  ++   ++     +   L 
Sbjct: 42  LCVTSLETNPQAKTAKDLAGLVMASTKNAVTKATTLKGTVDKIIKGKKVNKMTAMP--LR 101

Query: 92  DCLDEYLDASQQLDDSIAALLVKAYGDVDKWVNAAVAAVRTCEASFSVK--PSVLTPRND 151
           DCL  Y DA   L++++A +  + Y  V   ++AA+    TCE  F  +  PS +T  ND
Sbjct: 102 DCLQLYTDAIGSLNEALAGVKSRNYPTVKTVLSAAMDTPSTCETGFKERKAPSPVTKEND 161

Query: 152 NFIKL 154
           N  ++
Sbjct: 162 NLYQM 164

BLAST of MS019021 vs. TAIR 10
Match: AT3G12880.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 48.1 bits (113), Expect = 7.9e-06
Identity = 42/147 (28.57%), Postives = 65/147 (44.22%), Query Frame = 0

Query: 19  LIEQTCSQTQHPGLCLSSLKSDPNSFPADKTGLALVALRLVSSNASDISEGIKVMLNDTR 78
           L+ Q C  + +P LCL  L SDP S  ADK GLA + L+ ++S+   +++    +   ++
Sbjct: 31  LMMQQCHNSDNPTLCLRCLSSDPGSHEADKVGLARIILKCINSHLLVLTKNASTL--GSQ 90

Query: 79  LADQPGVQQGLYDCLDEYLDASQQLDDSIAALLV----KAYGDVD-KWVNAAVAAVRTCE 138
               P     L  C   +  A   + ++   L+     KA  DV  K VN  V+   + E
Sbjct: 91  HYQNPNTAAALKQCGLGFSTAKHGVGEADTQLITGDYDKAAYDVSVKVVNPPVSCRNSLE 150

Query: 139 ASFSVKPSVLTPRNDNFIKLCQNALTI 161
           A     PS      D ++ L Q  L I
Sbjct: 151 ALNIQVPSSFRYHMDVYLALTQALLRI 175

BLAST of MS019021 vs. TAIR 10
Match: AT5G46930.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 47.4 bits (111), Expect = 1.3e-05
Identity = 42/141 (29.79%), Postives = 69/141 (48.94%), Query Frame = 0

Query: 19  LIEQTCSQ--TQHPGL----CLSSLKSDPNSFPADKTGLALVALRLVSS-NASDISEGIK 78
           LI  +C +  T+ P L    C+ SL+ +P S    KT  +L  L  VS+ NA   +  +K
Sbjct: 23  LIRDSCKKATTKDPKLKYDFCVKSLEENPQS----KTAKSLEGLVFVSTKNAVSKTTSLK 82

Query: 79  VMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDSIAALLVKAYGDVDKWVNAAVAAVRT 138
            M++     D+  V++ L DCL+ Y +A   L+ S+  +  + Y      ++AA+ A  +
Sbjct: 83  GMVDKILKEDKYEVERPLLDCLELYTEAIDSLNQSLDTVKSRDYKTATMLMSAAMDAPGS 142

Query: 139 CEASFSVK----PSVLTPRND 149
           CE  F+ +     S  T  ND
Sbjct: 143 CETKFTKRKKAVKSPFTKEND 159