MS019016 (gene) Bitter gourd (TR) v1

Overview


Name	MS019016
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Fumarate hydratase
Location	scaffold20: 733565 .. 738634 (+)
RNA-Seq Expression	MS019016
Synteny	MS019016

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGCCGCGTATGTTCTGTCCCGGCGATCTTCGCCTTCGCACCTGTTCGGGGTGGCGCGTTGTGCTACTGCGTCTTTGAGATCTTACTCCACGTCTTTTAGGGAGGAAAGAGATACGTTTGGACCGATTAACGTTCCCTCCGACAGGTTCGTGTGTGTCTCTCTCTCTCTCGTTCATCTGGTTTTTGGATTTTCTTGACTAACACTTGCTTTTGTTTTCCATTTCCTCAAAGGAAATTGTGATAAATGAAGTGAAATAAGTATTTTAATTACGTTGTTTGCCTTTTTAGATTATGGGGAGCTCAAACGCAGAGATCGTTGCAGAACTTCGAGATCGGAGGAGACCGTGAGCGGATGCCTGAACCTATAGTTCGTGCCTTTGGCGTACTTAAAAAGTGTGCTGCCAAGGTCCATTCGTTTTCAAATTAAGTTATAATGTGATGTTTGATTTTTTGTGCTACAGTTCGTATTTTGCAACTGAGATGAGATATTATTGCTTGGAAATGGTTCTACTGTTCATATTGAATCTGTTTTTGTAGGTAAACATGGAATATGGTCTTGATCCAACTATTGGGAAAGCCATAATGCAAGCAGCCCAAGAAGTAGCCGAGGGAAAGTTAAATGATCACTTTCCGCTAGTTGTATGGCAAACTGGAAGTGGCACTCAGAGTAACATGAATGCCAATGAGGTTAGGAGAACTACTCTGTTTTATCTTGATTGTGAGGTGCCAAGAGCGAAGGTTCAATCTATTAAGGATCTTATTGAAAGTCTTACATATTTCTTTCTTTTACATGAATCTCTTTCTTTGATCACTTTGTCGTGGTATTTTTTACCCAAGGAAGCATGAAAGGCTAGGTCACTGTTTTTATGTTAGGTTTGAGTTCTTATTAACGGCAGATATGATGGCATGTGGTATTTAATCCCTTAATATACAACTCTCCCATGGGAGAGATTTGCTTTCCTTGGAAAATTAGGTTCCGTCAATCGCCATTCTTTAATCTAATGCAAAGTTCAAATCTAATTATTTCCTGAACTCCATGGCTTAAATTTATAATGCCATGTACGTGAAGTATGCGGCTTCCAAACTAGTGGTTACTGATGGTTGAATTGAAGACGTTGTAAGATGACTACAGTTTTTTTCACCCCCTCCCTTCCCTATGTAGTCAAATTCACCGAGTCTTCCTAAAGTACAGTCTCTTGAGAACAGAAAAGCATTTCCTCCAATAAGATATACATTTCCCTTCAATAGTATTTGAAGTATCTAATATGAATGTTATGCGCACATGATTGCAGAACATAGGGTAATATGTTGAGTAATCTTCTTATAATGTCTCTGAGGGTATATTTTGTTAGTTGATTACTTGAATGGTACAAAATTGTGGACGTATTATTTTTGTGGCAGGTCATTGCTAACAGAGCATCAGAGATCCTTGGCCATAAACGTGGTGAGAAGTTTGTGCACCCGAATGACCATGTTAACCGGTCACAATCTTCTAATGATACATTCCCAACTGTTAGTCATTTGCTTTCTTAAGTTATTTTGTGTATTGTTATTCCAACTCTTTTTCTGTAAATAACCAATAAAGTTTAGCAGTTTTCCATGGCAAAACTTCTTTTTTCCATAGAAGTTTTAATGATCAAAGTTTATGCCATTACTACTTTGGTCAAATTTTTATGCCATTACTACTTTTATTTGTCTCAATTGACAAGAGTTTTTTCCTCACTTCTGATGATTTCTACTGATTTCCTTTTACAGGTAATGCACATAGCAGCTGCAACAGAAATTAATTCCAGATTCATACCAAAATTGAAAACTTTGCATTCATCACTAAACTCAAAGGTTTGATTTTTATTAGAGATACGATGCCCAGAATTTCAAATTTTTGTTTGCATCTTCACATGTTTTAAATAATCCGAGCTTAATTTATTTACAGAAGCCTGTTTGAGTCATAATTTTAGGTCATGTTTGTTTGATTGCATGGATTAAGCTGAATGTCTAAAAAGCATGGAAAGCATTAGGAGATCATAACATAGTTTGAATCTGATTTACCATCTGTTAGTTTGAACACTTCTTAACTGAGTTGTATGGTAGTGATTGTATGTGTTTATTTACACTCCCTGCTCCTTTGCATTTTATTTGCCCTTTGCACCTTAATTTAAGTCTGTAATTCCTTATATTACTCTGTTATGATCCAAACAGTCCATTGAATTCAAAGATATTGTTAAGATTGGACGCACTCACACTCAAGATGCCACACCATTAACCCTTGGGCAAGAGTTCAGCGGCTATACCACACAAGTATGCTTTCTTTCTCTTTGTGGTTGTGAAAGTTTTGGTGGTCCTGATGCCTATTGAAAAGAAGAAATAGAGTACGATGCATATAAATAATTTTCTGATTGTTGTGTGCTTGGGCTGCAGGTGAAGTATGGTATTGACAGAGTCTTGTGCACTCTACCTCGAATGTATCAGGTTGTGATGCTTGTAATTGTTCCAGGTATTTTTTCAATTCCTTTTGCTATTTTTATTTTTGACACTTCAGAATTCCATTTGTACAGCTTGCCCAAGGTGGAACTGCTGTTGGAACTGGATTGAACACAAAGAAAGGGTATATTTTCTTTCGAATTAGTATGAATAAGAAAAAATATCTAGTTTTCTTATATTGTGCTTCAAGACCAGAAGGATGAGCGTATTTCTTATTTTGAGATTGGCGCTGTCTATTATTCAGATTTGATGTGAAGATAGCTGCAGCAGTAGCTGAAGAAACAAATTTCCCGTTTGTGACGGCAGAAAACAAGTTTGAAGCTCTGGTTAGTCAATTGAAGGAGCATCATCTTCCCTCTCTTTTGTTTTTTATTGCACAGTTTGGTGTTGCCTTCCTTCATACATGGAACCATTTTTTTATGTTGGAATATTTTGTTTTGCTAGGCGGCACATGACGCTTTTGTTGAGACTAGTGGGGCTCTTAACACAGTTGCTACTTCCTTGATGAAGATTGCTAATGACATACGCCTGTTGGGAAGGTTTATATGCATTAATTTCGTCGATCCTAGCACTGTATCATTTCTTGTCTTTCTTTATCTAATATTTCCCCCCTCATTTCATGTATACAGTGGTCCACGTTGTGGTCTTGGTGAACTTATTCTTCCCGAAAATGAGCCAGGCAGTAGTATTATGCCAGTAAATCTCTCTGTCTCTCTCTCTGTGTTTCGGGGGCACATGCGTGTCTGTCTGTCTTTCAGAAGCTATGTTTCAATGCTTTGGGCTTGTTAAGCTTAACAATTTTTTCTGGTTCTAGGGGAAGGTCAACCCTACTCAGTGCGAGGCTCTCACTATGGTCTGTGCTCAGGTATAGTTACCTTCTAGCATAGTTCATTATGTGATGCTGAAAGAGGAATTAGTTAATATAGATCCAACTAATTATGTTGGTTTCCATTTGTTCCCCAAAAATTTTGAGTTACCATTCTAAGCTATGCATACTCTGGTTATCAGTGACTAGTTTCATATATGTAATATATTGTCATTCTTGAACCTTAATTAGTTCAATGTGTTCCAACAAATTTTTGTTAATATTTTGTTTATTTGTTTTAAATAAATCAATTAAAAGTTTGTTTCTAATTTTTTAATTAAAAAAAAATATATATTAGATGCGATCGACAGTTCTTCATGTTTTACAGGTTATGGGAAACCATGTGGCAGTAACGGTTGGTGGATCAAATGGACACTTTGAGCTTAATGTATTTAAGCCAATGATCGCCAATGCTCTTTTGCATGTAAGTTCTGTAGTATCTCTTCATCGTTGTTATTTCTAGATATTATGTTTAAATAATGATATCATAAACTAAACTATTATCTTGTGGTTGCACCATCTGCTATGAGATTCATTTATCATATTGTCGATCACTCGATCTGCTTATCGTCCTCAAATGACATGCACGCACGCACATATATATATAGAGTTAGAAATAAATATTCTGGATTTATTGAACCTTCATCTCGAACTCTGGCTCTGCAGTCATTGAGATTATTAGGAGATGCTTCTGCTTCATTTGAAAAGAACTGTGTAAGAGGAATTCAAGCTAACACGGAAAGAATTTCGAAATTATTGCGTGAGGTTAGTTTTATGTTAATATTTGTAAAAGATAATTTTATGCTACAACGCTTGTGTTGAAGATTTGGCTGATGATGATTCTTGTGTATATCTCTGCACTTAAATTCGATGGCCTTGCAGTCGCTGATGCTCGTCACAGCTTTGAACCCTGTAAGTGATCATAACTAGAAAGCTCTCATTGTCCTTAGGCTTGGAACATTGTTTGATAACGTGTTCATGCATACTGTTTCCAGATAATTGGTTACGACAATGCTGCTGCAGTTGCAAAGACAGCCCACAAGGAAGGATCCACTTTGAAGGTATGAATCTCGTTTGGATTACATTTATAATCCTAATTGTATAGGTTTAAATCTTATTTGCATCTCTAAACGTTTCATGCTTATACTGTTTCTAGTCTTTAAACTTTCAAACATATTTTTTTATCTCTAAAGTTGTGCATAAAGAACTATTTAGTCAAAGTCACCCATTTTTAAATAAGTTAGTTAATAGAAGTTTGATATGGGTATCTCCTTATTCATTAGCCTAATCAATAGACATCTGTACGAACAAGTGTTTGAAGTCATATGTTGTTGGTTGGGTGTCAAATGACTGAAGTAGGTTTTTTTTCTTTTTTCCTAATGTTAAATTTAGGAACTTAGACAAAACATTAGATAGTCCGGACTAAAAATAGGACATTTGTAAGTTTAGATAATATATAAATACACAGACCAAAATAGGCATATATCTTTTTGTAGTACAACATGTGGGGATGGAGGATCGAACTCACAACCTCTTGGTTGGAGATATATGCCAATTACCGCTGAGCTAGCTTTGGTCCATATTTTTATTATTACCTAAATTGTCAAATGGCTCGTTTTGGTATATAATTCTGTAGGACGCTGCATTGAAGCTTGGAGTTCTAAGCGAGGAAGAGTTTGATAAACATGTGGTGCCTGAGAAGATGATTGGTCCATCTGAT

mRNA sequence

ATGGCTGCCGCGTATGTTCTGTCCCGGCGATCTTCGCCTTCGCACCTGTTCGGGGTGGCGCGTTGTGCTACTGCGTCTTTGAGATCTTACTCCACGTCTTTTAGGGAGGAAAGAGATACGTTTGGACCGATTAACGTTCCCTCCGACAGATTATGGGGAGCTCAAACGCAGAGATCGTTGCAGAACTTCGAGATCGGAGGAGACCGTGAGCGGATGCCTGAACCTATAGTTCGTGCCTTTGGCGTACTTAAAAAGTGTGCTGCCAAGGTAAACATGGAATATGGTCTTGATCCAACTATTGGGAAAGCCATAATGCAAGCAGCCCAAGAAGTAGCCGAGGGAAAGTTAAATGATCACTTTCCGCTAGTTGTATGGCAAACTGGAAGTGGCACTCAGAGTAACATGAATGCCAATGAGGTCATTGCTAACAGAGCATCAGAGATCCTTGGCCATAAACGTGGTGAGAAGTTTGTGCACCCGAATGACCATGTTAACCGGTCACAATCTTCTAATGATACATTCCCAACTGTAATGCACATAGCAGCTGCAACAGAAATTAATTCCAGATTCATACCAAAATTGAAAACTTTGCATTCATCACTAAACTCAAAGTCCATTGAATTCAAAGATATTGTTAAGATTGGACGCACTCACACTCAAGATGCCACACCATTAACCCTTGGGCAAGAGTTCAGCGGCTATACCACACAAGTGAAGTATGGTATTGACAGAGTCTTGTGCACTCTACCTCGAATGTATCAGCTTGCCCAAGGTGGAACTGCTGTTGGAACTGGATTGAACACAAAGAAAGGATTTGATGTGAAGATAGCTGCAGCAGTAGCTGAAGAAACAAATTTCCCGTTTGTGACGGCAGAAAACAAGTTTGAAGCTCTGGCGGCACATGACGCTTTTGTTGAGACTAGTGGGGCTCTTAACACAGTTGCTACTTCCTTGATGAAGATTGCTAATGACATACGCCTGTTGGGAAGTGGTCCACGTTGTGGTCTTGGTGAACTTATTCTTCCCGAAAATGAGCCAGGCAGTAGTATTATGCCAGGGAAGGTCAACCCTACTCAGTGCGAGGCTCTCACTATGGTCTGTGCTCAGGTTATGGGAAACCATGTGGCAGTAACGGTTGGTGGATCAAATGGACACTTTGAGCTTAATGTATTTAAGCCAATGATCGCCAATGCTCTTTTGCATTCATTGAGATTATTAGGAGATGCTTCTGCTTCATTTGAAAAGAACTGTGTAAGAGGAATTCAAGCTAACACGGAAAGAATTTCGAAATTATTGCGTGAGGTTAGTTTTATGTTAATATTTATAATTGGTTACGACAATGCTGCTGCAGTTGCAAAGACAGCCCACAAGGAAGGATCCACTTTGAAGGACGCTGCATTGAAGCTTGGAGTTCTAAGCGAGGAAGAGTTTGATAAACATGTGGTGCCTGAGAAGATGATTGGTCCATCTGAT

Coding sequence (CDS)

ATGGCTGCCGCGTATGTTCTGTCCCGGCGATCTTCGCCTTCGCACCTGTTCGGGGTGGCGCGTTGTGCTACTGCGTCTTTGAGATCTTACTCCACGTCTTTTAGGGAGGAAAGAGATACGTTTGGACCGATTAACGTTCCCTCCGACAGATTATGGGGAGCTCAAACGCAGAGATCGTTGCAGAACTTCGAGATCGGAGGAGACCGTGAGCGGATGCCTGAACCTATAGTTCGTGCCTTTGGCGTACTTAAAAAGTGTGCTGCCAAGGTAAACATGGAATATGGTCTTGATCCAACTATTGGGAAAGCCATAATGCAAGCAGCCCAAGAAGTAGCCGAGGGAAAGTTAAATGATCACTTTCCGCTAGTTGTATGGCAAACTGGAAGTGGCACTCAGAGTAACATGAATGCCAATGAGGTCATTGCTAACAGAGCATCAGAGATCCTTGGCCATAAACGTGGTGAGAAGTTTGTGCACCCGAATGACCATGTTAACCGGTCACAATCTTCTAATGATACATTCCCAACTGTAATGCACATAGCAGCTGCAACAGAAATTAATTCCAGATTCATACCAAAATTGAAAACTTTGCATTCATCACTAAACTCAAAGTCCATTGAATTCAAAGATATTGTTAAGATTGGACGCACTCACACTCAAGATGCCACACCATTAACCCTTGGGCAAGAGTTCAGCGGCTATACCACACAAGTGAAGTATGGTATTGACAGAGTCTTGTGCACTCTACCTCGAATGTATCAGCTTGCCCAAGGTGGAACTGCTGTTGGAACTGGATTGAACACAAAGAAAGGATTTGATGTGAAGATAGCTGCAGCAGTAGCTGAAGAAACAAATTTCCCGTTTGTGACGGCAGAAAACAAGTTTGAAGCTCTGGCGGCACATGACGCTTTTGTTGAGACTAGTGGGGCTCTTAACACAGTTGCTACTTCCTTGATGAAGATTGCTAATGACATACGCCTGTTGGGAAGTGGTCCACGTTGTGGTCTTGGTGAACTTATTCTTCCCGAAAATGAGCCAGGCAGTAGTATTATGCCAGGGAAGGTCAACCCTACTCAGTGCGAGGCTCTCACTATGGTCTGTGCTCAGGTTATGGGAAACCATGTGGCAGTAACGGTTGGTGGATCAAATGGACACTTTGAGCTTAATGTATTTAAGCCAATGATCGCCAATGCTCTTTTGCATTCATTGAGATTATTAGGAGATGCTTCTGCTTCATTTGAAAAGAACTGTGTAAGAGGAATTCAAGCTAACACGGAAAGAATTTCGAAATTATTGCGTGAGGTTAGTTTTATGTTAATATTTATAATTGGTTACGACAATGCTGCTGCAGTTGCAAAGACAGCCCACAAGGAAGGATCCACTTTGAAGGACGCTGCATTGAAGCTTGGAGTTCTAAGCGAGGAAGAGTTTGATAAACATGTGGTGCCTGAGAAGATGATTGGTCCATCTGAT

Protein sequence

MAAAYVLSRRSSPSHLFGVARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHIAAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRGIQANTERISKLLREVSFMLIFIIGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEFDKHVVPEKMIGPSD

Homology

[Close]

BLAST of MS019016 vs. NCBI nr
Match: XP_022155265.1 (fumarate hydratase 1, mitochondrial [Momordica charantia])

HSP 1 Score: 949.5 bits (2453), Expect = 1.1e-272
Identity = 485/495 (97.98%), Postives = 487/495 (98.38%), Query Frame = 0


Query: 1   MAAAYVLSRRSSPSHLFGVARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAAAYVLSRRSSPSHLFG+ARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAAAYVLSRRSSPSHLFGMARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAA 300
           GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAA
Sbjct: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAA 300

Query: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420

Query: 421 IQANTERISKLLREVSFMLIFI----IGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEE 480
           IQANTERISKLLRE S ML+      IGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEE
Sbjct: 421 IQANTERISKLLRE-SLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEE 480

Query: 481 FDKHVVPEKMIGPSD 492
           FDKHVVPEKMIGPSD
Sbjct: 481 FDKHVVPEKMIGPSD 494

[Close]

BLAST of MS019016 vs. NCBI nr
Match: XP_022984048.1 (fumarate hydratase 1, mitochondrial-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 922.9 bits (2384), Expect = 1.1e-264
Identity = 471/495 (95.15%), Postives = 481/495 (97.17%), Query Frame = 0


Query: 1   MAAAYVLSRRSSPSHLFGVARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAA YVLSRRSSPSHLFG+AR AT SLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAALYVLSRRSSPSHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPI+RAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINS+FIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSKFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAA 300
           GIDRVL TLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPF+TAENKFEALAA
Sbjct: 241 GIDRVLRTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFLTAENKFEALAA 300

Query: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVA+TVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420

Query: 421 IQANTERISKLLREVSFMLIFI----IGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEE 480
           IQAN ERISKLL E S ML+      IGYDNAAAVAKTAHKEGSTLK+AALKLGVLSEE+
Sbjct: 421 IQANRERISKLLHE-SLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAALKLGVLSEED 480

Query: 481 FDKHVVPEKMIGPSD 492
           F+K+VVPEKMIGPSD
Sbjct: 481 FNKYVVPEKMIGPSD 494

[Close]

BLAST of MS019016 vs. NCBI nr
Match: XP_023528894.1 (fumarate hydratase 1, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 919.5 bits (2375), Expect = 1.3e-263
Identity = 469/495 (94.75%), Postives = 479/495 (96.77%), Query Frame = 0


Query: 1   MAAAYVLSRRSSPSHLFGVARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAA YVLSRRSSPSHLFG+AR AT SLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAAVYVLSRRSSPSHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPI+RAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINS+FIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSKFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAA 300
           GIDRVL TLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETN PF+TAENKFEALAA
Sbjct: 241 GIDRVLRTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFLTAENKFEALAA 300

Query: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVA+TVGGSNGHFELNVFKPMIANALLHSLRLLGDAS SFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVFKPMIANALLHSLRLLGDASGSFEKNCVRG 420

Query: 421 IQANTERISKLLREVSFMLIFI----IGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEE 480
           IQAN ERISKLL E S ML+      IGYDNAAAVAKTAHKEGSTLK+AALKLGVLSEE+
Sbjct: 421 IQANRERISKLLHE-SLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAALKLGVLSEED 480

Query: 481 FDKHVVPEKMIGPSD 492
           F+K+VVPEKMIGPSD
Sbjct: 481 FNKYVVPEKMIGPSD 494

[Close]

BLAST of MS019016 vs. NCBI nr
Match: KAA0044396.1 (fumarate hydratase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 916.4 bits (2367), Expect = 1.1e-262
Identity = 466/494 (94.33%), Postives = 475/494 (96.15%), Query Frame = 0


Query: 1   MAAAYVLSRRSSPSHLFGVARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAA YVLSRRSSPSHLFG+AR AT SLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAAVYVLSRRSSPSHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPI+RAFGVLKKCAAKVNMEYGLDPT+GKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTVGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINS+FIPKLKTLHSSL SKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAA 300
           GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETN PFVTAENKFEALAA
Sbjct: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAA 300

Query: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSG+LNTVA SLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVA+TVGGSNGHFELNV+KPMIANALLHSLRLLGDASASFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG 420

Query: 421 IQANTERISKLLREVSFMLIFI----IGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEE 480
           IQAN ERISKLL E S ML+      IGYDNAAAVAK AHKEG TLK+AALKLGVL EEE
Sbjct: 421 IQANRERISKLLHE-SLMLVTALNPKIGYDNAAAVAKKAHKEGCTLKEAALKLGVLCEEE 480

Query: 481 FDKHVVPEKMIGPS 491
           FDKHVVPEKMIGPS
Sbjct: 481 FDKHVVPEKMIGPS 493

[Close]

BLAST of MS019016 vs. NCBI nr
Match: XP_004152237.1 (fumarate hydratase 1, mitochondrial [Cucumis sativus] >KGN52832.1 hypothetical protein Csa_014636 [Cucumis sativus])

HSP 1 Score: 916.0 bits (2366), Expect = 1.4e-262
Identity = 466/495 (94.14%), Postives = 477/495 (96.36%), Query Frame = 0


Query: 1   MAAAYVLSRRSSPSHLFGVARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAA YVLSRRSS SHLFG+AR AT SLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAAVYVLSRRSSASHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPI+RAFGVLKKCAAKVNM+YGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMDYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRG+KFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGDKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINS+FIPKLKTLHSSL SKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAA 300
           GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETN PFVTAENKFEALAA
Sbjct: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAA 300

Query: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSG+LNTVA SLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVA+TVGGSNGHFELNV+KPMIANALLHSLRLLGDASASFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG 420

Query: 421 IQANTERISKLLREVSFMLIFI----IGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEE 480
           IQAN ERISKLL E S ML+      IGYDNAAAVAK AHKEGSTLK+AALKLGVLSEE+
Sbjct: 421 IQANRERISKLLHE-SLMLVTALNPKIGYDNAAAVAKKAHKEGSTLKEAALKLGVLSEED 480

Query: 481 FDKHVVPEKMIGPSD 492
           FDKHVVPEKMIGPSD
Sbjct: 481 FDKHVVPEKMIGPSD 494

[Close]

BLAST of MS019016 vs. ExPASy Swiss-Prot
Match: P93033 (Fumarate hydratase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FUM1 PE=1 SV=2)

HSP 1 Score: 839.7 bits (2168), Expect = 1.7e-242
Identity = 431/491 (87.78%), Postives = 449/491 (91.45%), Query Frame = 0


Query: 5   YVLSRRSSPSHLFGVARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSLQNFE 64
           YV SRR S        R AT SLRSYSTSFREERDTFGPI VPSD+LWGAQTQRSLQNFE
Sbjct: 4   YVASRRLSGGTTVTALRYAT-SLRSYSTSFREERDTFGPIQVPSDKLWGAQTQRSLQNFE 63

Query: 65  IGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVV 124
           IGG+RERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVV
Sbjct: 64  IGGERERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVV 123

Query: 125 WQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHIAAAT 184
           WQTGSGTQSNMNANEVIANRA+EILG KRGEK VHPNDHVNRSQSSNDTFPTVMHIAAAT
Sbjct: 124 WQTGSGTQSNMNANEVIANRAAEILGRKRGEKCVHPNDHVNRSQSSNDTFPTVMHIAAAT 183

Query: 185 EINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDR 244
           EINSR IP LKTLHS+L SKS EFKDIVKIGRTHTQDATPLTLGQEF GY TQVKYG++R
Sbjct: 184 EINSRLIPSLKTLHSTLESKSFEFKDIVKIGRTHTQDATPLTLGQEFGGYATQVKYGLNR 243

Query: 245 VLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAAHDAF 304
           V CTLPR+YQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETN PFVTAENKFEALAAHDA 
Sbjct: 244 VTCTLPRLYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDAC 303

Query: 305 VETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALT 364
           VETSG+LNT+ATSLMKIANDIR LGSGPRCGLGEL+LPENEPGSSIMPGKVNPTQCEALT
Sbjct: 304 VETSGSLNTIATSLMKIANDIRFLGSGPRCGLGELVLPENEPGSSIMPGKVNPTQCEALT 363

Query: 365 MVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRGIQAN 424
           MVCAQVMGNHVAVTVGGSNGHFELNVFKP+IA+ALLHS+RL+ DASASFEKNCVRGI+AN
Sbjct: 364 MVCAQVMGNHVAVTVGGSNGHFELNVFKPVIASALLHSVRLIADASASFEKNCVRGIEAN 423

Query: 425 TERISKLLREVSFMLIFI----IGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEFDKH 484
            ERISKLL E S ML+      IGYDNAAAVAK AHKEG TLK+AAL LGVL+ EEFD  
Sbjct: 424 RERISKLLHE-SLMLVTSLNPKIGYDNAAAVAKKAHKEGCTLKEAALNLGVLTAEEFDTL 483

Query: 485 VVPEKMIGPSD 492
           VVPEKMIGPSD
Sbjct: 484 VVPEKMIGPSD 492

[Close]

BLAST of MS019016 vs. ExPASy Swiss-Prot
Match: Q9FI53 (Fumarate hydratase 2 OS=Arabidopsis thaliana OX=3702 GN=FUM2 PE=1 SV=1)

HSP 1 Score: 822.4 bits (2123), Expect = 2.7e-237
Identity = 415/468 (88.68%), Postives = 434/468 (92.74%), Query Frame = 0


Query: 28  RSYSTSFREERDTFGPINVPSDRLWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCA 87
           RSYST FREERDTFGPI VPSD+LWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCA
Sbjct: 33  RSYSTPFREERDTFGPIQVPSDKLWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCA 92

Query: 88  AKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRASE 147
           AKVNMEYGLDP IG+AIM+AAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRA+E
Sbjct: 93  AKVNMEYGLDPMIGEAIMEAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRAAE 152

Query: 148 ILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHIAAATEINSRFIPKLKTLHSSLNSKSIE 207
           ILGHKRGEK VHPNDHVNRSQSSNDTFPTVMHIAAATEI SR IP LK LHSSL SKS E
Sbjct: 153 ILGHKRGEKIVHPNDHVNRSQSSNDTFPTVMHIAAATEITSRLIPSLKNLHSSLESKSFE 212

Query: 208 FKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLN 267
           FKDIVKIGRTHTQDATPLTLGQEF GY TQV+YG++RV CTLPR+YQLAQGGTAVGTGLN
Sbjct: 213 FKDIVKIGRTHTQDATPLTLGQEFGGYATQVEYGLNRVACTLPRIYQLAQGGTAVGTGLN 272

Query: 268 TKKGFDVKIAAAVAEETNFPFVTAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRL 327
           TKKGFDVKIAAAVAEETN PFVTAENKFEALAAHDA VETSG+LNT+ATSLMKIANDIR 
Sbjct: 273 TKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDACVETSGSLNTIATSLMKIANDIRF 332

Query: 328 LGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFE 387
           LGSGPRCGLGEL LPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVT+GGSNGHFE
Sbjct: 333 LGSGPRCGLGELSLPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTIGGSNGHFE 392

Query: 388 LNVFKPMIANALLHSLRLLGDASASFEKNCVRGIQANTERISKLLREVSFMLIFI----I 447
           LNVFKP+IA+ALLHS+RL+ DASASFEKNCVRGI+AN ERISKLL E S ML+      I
Sbjct: 393 LNVFKPVIASALLHSIRLIADASASFEKNCVRGIEANRERISKLLHE-SLMLVTSLNPKI 452

Query: 448 GYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEFDKHVVPEKMIGPSD 492
           GYDNAAAVAK AHKEG TLK AA+KLGVL+ EEFD  VVPEKMIGPSD
Sbjct: 453 GYDNAAAVAKRAHKEGCTLKHAAMKLGVLTSEEFDTLVVPEKMIGPSD 499

[Close]

BLAST of MS019016 vs. ExPASy Swiss-Prot
Match: P14408 (Fumarate hydratase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Fh PE=1 SV=2)

HSP 1 Score: 649.8 bits (1675), Expect = 2.4e-185
Identity = 331/475 (69.68%), Postives = 385/475 (81.05%), Query Frame = 0


Query: 21  RCATASLRSYS-TSFREERDTFGPINVPSDRLWGAQTQRSLQNFEIGGDRERMPEPIVRA 80
           RCA   +R  S  SFR E DTFG + VP+D+ +GAQT RS  NF+IGG  ERMP P+++A
Sbjct: 33  RCAPNVVRMASQNSFRIEYDTFGELKVPTDKYYGAQTVRSTMNFKIGGATERMPIPVIKA 92

Query: 81  FGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANE 140
           FG+LK+ AA+VN EYGLDP I  AIM+AA EVAEGKLNDHFPLVVWQTGSGTQ+NMN NE
Sbjct: 93  FGILKRAAAEVNQEYGLDPKIASAIMKAADEVAEGKLNDHFPLVVWQTGSGTQTNMNVNE 152

Query: 141 VIANRASEILGHKRG-EKFVHPNDHVNRSQSSNDTFPTVMHIAAATEINSRFIPKLKTLH 200
           VI+NRA E+LG + G +K VHPNDHVN+SQSSNDTFPT MHIAAA E++   +P L+ LH
Sbjct: 153 VISNRAIEMLGGELGSKKPVHPNDHVNKSQSSNDTFPTAMHIAAALEVHQVLLPGLQKLH 212

Query: 201 SSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDRVLCTLPRMYQLAQG 260
            +L++KS EF  ++KIGRTHTQDA PLTLGQEFSGY  QV+Y ++R+   +PR+Y+LA G
Sbjct: 213 DALSAKSKEFAQVIKIGRTHTQDAVPLTLGQEFSGYVQQVQYAMERIKAAMPRIYELAAG 272

Query: 261 GTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAAHDAFVETSGALNTVATSL 320
           GTAVGTGLNT+ GF  K+AA VA  T  PFVTA NKFEALAAHDA VE SGA+NT A SL
Sbjct: 273 GTAVGTGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSL 332

Query: 321 MKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVT 380
           MKIANDIR LGSGPR GLGELILPENEPGSSIMPGKVNPTQCEA+TMV AQVMGNHVAVT
Sbjct: 333 MKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVT 392

Query: 381 VGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRGIQANTERISKLLREVSFM 440
           VGGSNGHFELNVFKPM+   +LHS RLLGDAS SF +NCV GIQANTERI+KL+ E S M
Sbjct: 393 VGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNE-SLM 452

Query: 441 LIFI----IGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEFDKHVVPEKMIGP 490
           L+      IGYD AA +AKTAHK GSTLK+ A++LG L+ E+FD+ V P+ M+GP
Sbjct: 453 LVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGP 506

[Close]

BLAST of MS019016 vs. ExPASy Swiss-Prot
Match: Q60HF9 (Fumarate hydratase, mitochondrial OS=Macaca fascicularis OX=9541 GN=FH PE=2 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 4.1e-185
Identity = 328/470 (69.79%), Postives = 380/470 (80.85%), Query Frame = 0


Query: 25  ASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLK 84
           A+  +   SFR E DTFG + VP+D+ +GAQT RS  NF+IGG  ERMP P+++AFG+LK
Sbjct: 41  AARMASQNSFRIEYDTFGELKVPNDKYYGAQTVRSTMNFKIGGVTERMPTPVIKAFGILK 100

Query: 85  KCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANR 144
           + AA+VN +YGLDP I  AIM+AA EVAEGKLNDHFPLVVWQTGSGTQ+NMN NEVI+NR
Sbjct: 101 RAAAEVNQDYGLDPKIANAIMKAADEVAEGKLNDHFPLVVWQTGSGTQTNMNVNEVISNR 160

Query: 145 ASEILGHKRGEKF-VHPNDHVNRSQSSNDTFPTVMHIAAATEINSRFIPKLKTLHSSLNS 204
           A E+LG + G K  VHPNDHVN+SQSSNDTFPT MHIAAA E++   +P L+ LH +L++
Sbjct: 161 AIEMLGGELGSKIPVHPNDHVNKSQSSNDTFPTAMHIAAAIEVHEVLLPGLQKLHDALDA 220

Query: 205 KSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVG 264
           KS EF  I+KIGRTHTQDA PLTLGQEFSGY  QVKY + R+   +PR+Y+LA GGTAVG
Sbjct: 221 KSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAVTRIKAAMPRIYELAAGGTAVG 280

Query: 265 TGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAAHDAFVETSGALNTVATSLMKIAN 324
           TGLNT+ GF  K+AA VA  T  PFVTA NKFEALAAHDA VE SGA+NT A SLMKIAN
Sbjct: 281 TGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIAN 340

Query: 325 DIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSN 384
           DIR LGSGPR GLGELILPENEPGSSIMPGKVNPTQCEA+TMV AQVMGNHVAVTVGGSN
Sbjct: 341 DIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSN 400

Query: 385 GHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRGIQANTERISKLLREVSFMLIFI- 444
           GHFELNVFKPM+   +LHS RLLGDAS SF +NCV GIQANTERI+KL+ E S ML+   
Sbjct: 401 GHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNE-SLMLVTAL 460

Query: 445 ---IGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEFDKHVVPEKMIGP 490
              IGYD AA +AKTAHK GSTLK+ A++LG L+ E+FD+ V P+ M+GP
Sbjct: 461 NPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGP 509

[Close]

BLAST of MS019016 vs. ExPASy Swiss-Prot
Match: P07954 (Fumarate hydratase, mitochondrial OS=Homo sapiens OX=9606 GN=FH PE=1 SV=3)

HSP 1 Score: 648.3 bits (1671), Expect = 7.1e-185
Identity = 328/470 (69.79%), Postives = 380/470 (80.85%), Query Frame = 0


Query: 25  ASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLK 84
           A+  +   SFR E DTFG + VP+D+ +GAQT RS  NF+IGG  ERMP P+++AFG+LK
Sbjct: 41  AARMASQNSFRIEYDTFGELKVPNDKYYGAQTVRSTMNFKIGGVTERMPTPVIKAFGILK 100

Query: 85  KCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANR 144
           + AA+VN +YGLDP I  AIM+AA EVAEGKLNDHFPLVVWQTGSGTQ+NMN NEVI+NR
Sbjct: 101 RAAAEVNQDYGLDPKIANAIMKAADEVAEGKLNDHFPLVVWQTGSGTQTNMNVNEVISNR 160

Query: 145 ASEILGHKRGEKF-VHPNDHVNRSQSSNDTFPTVMHIAAATEINSRFIPKLKTLHSSLNS 204
           A E+LG + G K  VHPNDHVN+SQSSNDTFPT MHIAAA E++   +P L+ LH +L++
Sbjct: 161 AIEMLGGELGSKIPVHPNDHVNKSQSSNDTFPTAMHIAAAIEVHEVLLPGLQKLHDALDA 220

Query: 205 KSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVG 264
           KS EF  I+KIGRTHTQDA PLTLGQEFSGY  QVKY + R+   +PR+Y+LA GGTAVG
Sbjct: 221 KSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAMTRIKAAMPRIYELAAGGTAVG 280

Query: 265 TGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAAHDAFVETSGALNTVATSLMKIAN 324
           TGLNT+ GF  K+AA VA  T  PFVTA NKFEALAAHDA VE SGA+NT A SLMKIAN
Sbjct: 281 TGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIAN 340

Query: 325 DIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSN 384
           DIR LGSGPR GLGELILPENEPGSSIMPGKVNPTQCEA+TMV AQVMGNHVAVTVGGSN
Sbjct: 341 DIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSN 400

Query: 385 GHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRGIQANTERISKLLREVSFMLIFI- 444
           GHFELNVFKPM+   +LHS RLLGDAS SF +NCV GIQANTERI+KL+ E S ML+   
Sbjct: 401 GHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNE-SLMLVTAL 460

Query: 445 ---IGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEFDKHVVPEKMIGP 490
              IGYD AA +AKTAHK GSTLK+ A++LG L+ E+FD+ V P+ M+GP
Sbjct: 461 NPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGP 509

[Close]

BLAST of MS019016 vs. ExPASy TrEMBL
Match: A0A6J1DLZ0 (Fumarate hydratase OS=Momordica charantia OX=3673 GN=LOC111022407 PE=3 SV=1)

HSP 1 Score: 949.5 bits (2453), Expect = 5.5e-273
Identity = 485/495 (97.98%), Postives = 487/495 (98.38%), Query Frame = 0


Query: 1   MAAAYVLSRRSSPSHLFGVARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAAAYVLSRRSSPSHLFG+ARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAAAYVLSRRSSPSHLFGMARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAA 300
           GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAA
Sbjct: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAA 300

Query: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420

Query: 421 IQANTERISKLLREVSFMLIFI----IGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEE 480
           IQANTERISKLLRE S ML+      IGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEE
Sbjct: 421 IQANTERISKLLRE-SLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEE 480

Query: 481 FDKHVVPEKMIGPSD 492
           FDKHVVPEKMIGPSD
Sbjct: 481 FDKHVVPEKMIGPSD 494

[Close]

BLAST of MS019016 vs. ExPASy TrEMBL
Match: A0A6J1J9G0 (Fumarate hydratase OS=Cucurbita maxima OX=3661 GN=LOC111482474 PE=3 SV=1)

HSP 1 Score: 922.9 bits (2384), Expect = 5.5e-265
Identity = 471/495 (95.15%), Postives = 481/495 (97.17%), Query Frame = 0


Query: 1   MAAAYVLSRRSSPSHLFGVARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAA YVLSRRSSPSHLFG+AR AT SLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAALYVLSRRSSPSHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPI+RAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINS+FIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSKFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAA 300
           GIDRVL TLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPF+TAENKFEALAA
Sbjct: 241 GIDRVLRTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFLTAENKFEALAA 300

Query: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVA+TVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420

Query: 421 IQANTERISKLLREVSFMLIFI----IGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEE 480
           IQAN ERISKLL E S ML+      IGYDNAAAVAKTAHKEGSTLK+AALKLGVLSEE+
Sbjct: 421 IQANRERISKLLHE-SLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAALKLGVLSEED 480

Query: 481 FDKHVVPEKMIGPSD 492
           F+K+VVPEKMIGPSD
Sbjct: 481 FNKYVVPEKMIGPSD 494

[Close]

BLAST of MS019016 vs. ExPASy TrEMBL
Match: A0A5A7TQL4 (Fumarate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G001250 PE=3 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 5.1e-263
Identity = 466/494 (94.33%), Postives = 475/494 (96.15%), Query Frame = 0


Query: 1   MAAAYVLSRRSSPSHLFGVARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAA YVLSRRSSPSHLFG+AR AT SLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAAVYVLSRRSSPSHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPI+RAFGVLKKCAAKVNMEYGLDPT+GKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTVGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINS+FIPKLKTLHSSL SKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAA 300
           GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETN PFVTAENKFEALAA
Sbjct: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAA 300

Query: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSG+LNTVA SLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVA+TVGGSNGHFELNV+KPMIANALLHSLRLLGDASASFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG 420

Query: 421 IQANTERISKLLREVSFMLIFI----IGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEE 480
           IQAN ERISKLL E S ML+      IGYDNAAAVAK AHKEG TLK+AALKLGVL EEE
Sbjct: 421 IQANRERISKLLHE-SLMLVTALNPKIGYDNAAAVAKKAHKEGCTLKEAALKLGVLCEEE 480

Query: 481 FDKHVVPEKMIGPS 491
           FDKHVVPEKMIGPS
Sbjct: 481 FDKHVVPEKMIGPS 493

[Close]

BLAST of MS019016 vs. ExPASy TrEMBL
Match: A0A0A0KWM0 (Fumarate hydratase OS=Cucumis sativus OX=3659 GN=Csa_4G001990 PE=3 SV=1)

HSP 1 Score: 916.0 bits (2366), Expect = 6.7e-263
Identity = 466/495 (94.14%), Postives = 477/495 (96.36%), Query Frame = 0


Query: 1   MAAAYVLSRRSSPSHLFGVARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAA YVLSRRSS SHLFG+AR AT SLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAAVYVLSRRSSASHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPI+RAFGVLKKCAAKVNM+YGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMDYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRG+KFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGDKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINS+FIPKLKTLHSSL SKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAA 300
           GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETN PFVTAENKFEALAA
Sbjct: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAA 300

Query: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSG+LNTVA SLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVA+TVGGSNGHFELNV+KPMIANALLHSLRLLGDASASFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG 420

Query: 421 IQANTERISKLLREVSFMLIFI----IGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEE 480
           IQAN ERISKLL E S ML+      IGYDNAAAVAK AHKEGSTLK+AALKLGVLSEE+
Sbjct: 421 IQANRERISKLLHE-SLMLVTALNPKIGYDNAAAVAKKAHKEGSTLKEAALKLGVLSEED 480

Query: 481 FDKHVVPEKMIGPSD 492
           FDKHVVPEKMIGPSD
Sbjct: 481 FDKHVVPEKMIGPSD 494

[Close]

BLAST of MS019016 vs. ExPASy TrEMBL
Match: A0A5D3E117 (Fumarate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001270 PE=3 SV=1)

HSP 1 Score: 915.6 bits (2365), Expect = 8.8e-263
Identity = 466/494 (94.33%), Postives = 475/494 (96.15%), Query Frame = 0


Query: 1   MAAAYVLSRRSSPSHLFGVARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAA YVLSRRSSPSHLFG+AR AT SLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAAVYVLSRRSSPSHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPI+RAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINS+FIPKLKTLHSSL SKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAA 300
           GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETN PF+TAENKFEALAA
Sbjct: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFMTAENKFEALAA 300

Query: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSG+LNTVA SLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVA+TVGGSNGHFELNV+KPMIANALLHSLRLLGDASASFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG 420

Query: 421 IQANTERISKLLREVSFMLIFI----IGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEE 480
           IQAN ERISKLL E S ML+      IGYDNAAAVAK AHKEG TLK+AALKLGVL EEE
Sbjct: 421 IQANRERISKLLHE-SLMLVTALNPKIGYDNAAAVAKKAHKEGCTLKEAALKLGVLCEEE 480

Query: 481 FDKHVVPEKMIGPS 491
           FDKHVVPEKMIGPS
Sbjct: 481 FDKHVVPEKMIGPS 493

[Close]

BLAST of MS019016 vs. TAIR 10
Match: AT2G47510.1 (fumarase 1 )

HSP 1 Score: 839.7 bits (2168), Expect = 1.2e-243
Identity = 431/491 (87.78%), Postives = 449/491 (91.45%), Query Frame = 0


Query: 5   YVLSRRSSPSHLFGVARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSLQNFE 64
           YV SRR S        R AT SLRSYSTSFREERDTFGPI VPSD+LWGAQTQRSLQNFE
Sbjct: 4   YVASRRLSGGTTVTALRYAT-SLRSYSTSFREERDTFGPIQVPSDKLWGAQTQRSLQNFE 63

Query: 65  IGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVV 124
           IGG+RERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVV
Sbjct: 64  IGGERERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVV 123

Query: 125 WQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHIAAAT 184
           WQTGSGTQSNMNANEVIANRA+EILG KRGEK VHPNDHVNRSQSSNDTFPTVMHIAAAT
Sbjct: 124 WQTGSGTQSNMNANEVIANRAAEILGRKRGEKCVHPNDHVNRSQSSNDTFPTVMHIAAAT 183

Query: 185 EINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDR 244
           EINSR IP LKTLHS+L SKS EFKDIVKIGRTHTQDATPLTLGQEF GY TQVKYG++R
Sbjct: 184 EINSRLIPSLKTLHSTLESKSFEFKDIVKIGRTHTQDATPLTLGQEFGGYATQVKYGLNR 243

Query: 245 VLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAAHDAF 304
           V CTLPR+YQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETN PFVTAENKFEALAAHDA 
Sbjct: 244 VTCTLPRLYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDAC 303

Query: 305 VETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALT 364
           VETSG+LNT+ATSLMKIANDIR LGSGPRCGLGEL+LPENEPGSSIMPGKVNPTQCEALT
Sbjct: 304 VETSGSLNTIATSLMKIANDIRFLGSGPRCGLGELVLPENEPGSSIMPGKVNPTQCEALT 363

Query: 365 MVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRGIQAN 424
           MVCAQVMGNHVAVTVGGSNGHFELNVFKP+IA+ALLHS+RL+ DASASFEKNCVRGI+AN
Sbjct: 364 MVCAQVMGNHVAVTVGGSNGHFELNVFKPVIASALLHSVRLIADASASFEKNCVRGIEAN 423

Query: 425 TERISKLLREVSFMLIFI----IGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEFDKH 484
            ERISKLL E S ML+      IGYDNAAAVAK AHKEG TLK+AAL LGVL+ EEFD  
Sbjct: 424 RERISKLLHE-SLMLVTSLNPKIGYDNAAAVAKKAHKEGCTLKEAALNLGVLTAEEFDTL 483

Query: 485 VVPEKMIGPSD 492
           VVPEKMIGPSD
Sbjct: 484 VVPEKMIGPSD 492

[Close]

BLAST of MS019016 vs. TAIR 10
Match: AT2G47510.2 (fumarase 1 )

HSP 1 Score: 839.7 bits (2168), Expect = 1.2e-243
Identity = 431/491 (87.78%), Postives = 449/491 (91.45%), Query Frame = 0


Query: 5   YVLSRRSSPSHLFGVARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSLQNFE 64
           YV SRR S        R AT SLRSYSTSFREERDTFGPI VPSD+LWGAQTQRSLQNFE
Sbjct: 4   YVASRRLSGGTTVTALRYAT-SLRSYSTSFREERDTFGPIQVPSDKLWGAQTQRSLQNFE 63

Query: 65  IGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVV 124
           IGG+RERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVV
Sbjct: 64  IGGERERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVV 123

Query: 125 WQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHIAAAT 184
           WQTGSGTQSNMNANEVIANRA+EILG KRGEK VHPNDHVNRSQSSNDTFPTVMHIAAAT
Sbjct: 124 WQTGSGTQSNMNANEVIANRAAEILGRKRGEKCVHPNDHVNRSQSSNDTFPTVMHIAAAT 183

Query: 185 EINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDR 244
           EINSR IP LKTLHS+L SKS EFKDIVKIGRTHTQDATPLTLGQEF GY TQVKYG++R
Sbjct: 184 EINSRLIPSLKTLHSTLESKSFEFKDIVKIGRTHTQDATPLTLGQEFGGYATQVKYGLNR 243

Query: 245 VLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAAHDAF 304
           V CTLPR+YQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETN PFVTAENKFEALAAHDA 
Sbjct: 244 VTCTLPRLYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDAC 303

Query: 305 VETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALT 364
           VETSG+LNT+ATSLMKIANDIR LGSGPRCGLGEL+LPENEPGSSIMPGKVNPTQCEALT
Sbjct: 304 VETSGSLNTIATSLMKIANDIRFLGSGPRCGLGELVLPENEPGSSIMPGKVNPTQCEALT 363

Query: 365 MVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRGIQAN 424
           MVCAQVMGNHVAVTVGGSNGHFELNVFKP+IA+ALLHS+RL+ DASASFEKNCVRGI+AN
Sbjct: 364 MVCAQVMGNHVAVTVGGSNGHFELNVFKPVIASALLHSVRLIADASASFEKNCVRGIEAN 423

Query: 425 TERISKLLREVSFMLIFI----IGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEFDKH 484
            ERISKLL E S ML+      IGYDNAAAVAK AHKEG TLK+AAL LGVL+ EEFD  
Sbjct: 424 RERISKLLHE-SLMLVTSLNPKIGYDNAAAVAKKAHKEGCTLKEAALNLGVLTAEEFDTL 483

Query: 485 VVPEKMIGPSD 492
           VVPEKMIGPSD
Sbjct: 484 VVPEKMIGPSD 492

[Close]

BLAST of MS019016 vs. TAIR 10
Match: AT5G50950.2 (FUMARASE 2 )

HSP 1 Score: 822.4 bits (2123), Expect = 1.9e-238
Identity = 415/468 (88.68%), Postives = 434/468 (92.74%), Query Frame = 0


Query: 28  RSYSTSFREERDTFGPINVPSDRLWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCA 87
           RSYST FREERDTFGPI VPSD+LWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCA
Sbjct: 33  RSYSTPFREERDTFGPIQVPSDKLWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCA 92

Query: 88  AKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRASE 147
           AKVNMEYGLDP IG+AIM+AAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRA+E
Sbjct: 93  AKVNMEYGLDPMIGEAIMEAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRAAE 152

Query: 148 ILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHIAAATEINSRFIPKLKTLHSSLNSKSIE 207
           ILGHKRGEK VHPNDHVNRSQSSNDTFPTVMHIAAATEI SR IP LK LHSSL SKS E
Sbjct: 153 ILGHKRGEKIVHPNDHVNRSQSSNDTFPTVMHIAAATEITSRLIPSLKNLHSSLESKSFE 212

Query: 208 FKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLN 267
           FKDIVKIGRTHTQDATPLTLGQEF GY TQV+YG++RV CTLPR+YQLAQGGTAVGTGLN
Sbjct: 213 FKDIVKIGRTHTQDATPLTLGQEFGGYATQVEYGLNRVACTLPRIYQLAQGGTAVGTGLN 272

Query: 268 TKKGFDVKIAAAVAEETNFPFVTAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRL 327
           TKKGFDVKIAAAVAEETN PFVTAENKFEALAAHDA VETSG+LNT+ATSLMKIANDIR 
Sbjct: 273 TKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDACVETSGSLNTIATSLMKIANDIRF 332

Query: 328 LGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFE 387
           LGSGPRCGLGEL LPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVT+GGSNGHFE
Sbjct: 333 LGSGPRCGLGELSLPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTIGGSNGHFE 392

Query: 388 LNVFKPMIANALLHSLRLLGDASASFEKNCVRGIQANTERISKLLREVSFMLIFI----I 447
           LNVFKP+IA+ALLHS+RL+ DASASFEKNCVRGI+AN ERISKLL E S ML+      I
Sbjct: 393 LNVFKPVIASALLHSIRLIADASASFEKNCVRGIEANRERISKLLHE-SLMLVTSLNPKI 452

Query: 448 GYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEFDKHVVPEKMIGPSD 492
           GYDNAAAVAK AHKEG TLK AA+KLGVL+ EEFD  VVPEKMIGPSD
Sbjct: 453 GYDNAAAVAKRAHKEGCTLKHAAMKLGVLTSEEFDTLVVPEKMIGPSD 499

[Close]

BLAST of MS019016 vs. TAIR 10
Match: AT5G50950.1 (FUMARASE 2 )

HSP 1 Score: 782.3 bits (2019), Expect = 2.2e-226
Identity = 397/456 (87.06%), Postives = 417/456 (91.45%), Query Frame = 0


Query: 28  RSYSTSFREERDTFGPINVPSDRLWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCA 87
           RSYST FREERDTFGPI VPSD+LWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCA
Sbjct: 33  RSYSTPFREERDTFGPIQVPSDKLWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCA 92

Query: 88  AKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRASE 147
           AKVNMEYGLDP IG+AIM+AAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRA+E
Sbjct: 93  AKVNMEYGLDPMIGEAIMEAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRAAE 152

Query: 148 ILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHIAAATEINSRFIPKLKTLHSSLNSKSIE 207
           ILGHKRGEK VHPNDHVNRSQSSNDTFPTVMHIAAATEI SR IP LK LHSSL SKS E
Sbjct: 153 ILGHKRGEKIVHPNDHVNRSQSSNDTFPTVMHIAAATEITSRLIPSLKNLHSSLESKSFE 212

Query: 208 FKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLN 267
           FKDIVKIGRTHTQDATPLTLGQEF GY TQV+YG++RV CTLPR+YQLAQGGTAVGTGLN
Sbjct: 213 FKDIVKIGRTHTQDATPLTLGQEFGGYATQVEYGLNRVACTLPRIYQLAQGGTAVGTGLN 272

Query: 268 TKKGFDVKIAAAVAEETNFPFVTAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRL 327
           TKKGFDVKIAAAVAEETN PFVTAENKFEALAAHDA VETSG+LNT+ATSLMKIANDIR 
Sbjct: 273 TKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDACVETSGSLNTIATSLMKIANDIRF 332

Query: 328 LGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFE 387
           LGSGPRCGLGEL LPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVT+GGSNGHFE
Sbjct: 333 LGSGPRCGLGELSLPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTIGGSNGHFE 392

Query: 388 LNVFKPMIANALLHSLRLLGDASASFEKNCVRGIQANTERISKLLREVSFMLIFI----I 447
           LNVFKP+IA+ALLHS+RL+ DASASFEKNCVRGI+AN ERISKLL E S ML+      I
Sbjct: 393 LNVFKPVIASALLHSIRLIADASASFEKNCVRGIEANRERISKLLHE-SLMLVTSLNPKI 452

Query: 448 GYDNAAAVAKTAHKEGSTLK--DAALKLGVLSEEEF 478
           GYDNAAAVAK AHKEG TLK  +  L    L++ EF
Sbjct: 453 GYDNAAAVAKRAHKEGCTLKVNNKLLTFSSLNKSEF 487

[Close]

BLAST of MS019016 vs. TAIR 10
Match: AT5G50950.3 (FUMARASE 2 )

HSP 1 Score: 737.3 bits (1902), Expect = 8.2e-213
Identity = 374/424 (88.21%), Postives = 392/424 (92.45%), Query Frame = 0


Query: 72  MPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGT 131
           MPEPIVRAFGVLKKCAAKVNMEYGLDP IG+AIM+AAQEVAEGKLNDHFPLVVWQTGSGT
Sbjct: 1   MPEPIVRAFGVLKKCAAKVNMEYGLDPMIGEAIMEAAQEVAEGKLNDHFPLVVWQTGSGT 60

Query: 132 QSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHIAAATEINSRFI 191
           QSNMNANEVIANRA+EILGHKRGEK VHPNDHVNRSQSSNDTFPTVMHIAAATEI SR I
Sbjct: 61  QSNMNANEVIANRAAEILGHKRGEKIVHPNDHVNRSQSSNDTFPTVMHIAAATEITSRLI 120

Query: 192 PKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDRVLCTLPR 251
           P LK LHSSL SKS EFKDIVKIGRTHTQDATPLTLGQEF GY TQV+YG++RV CTLPR
Sbjct: 121 PSLKNLHSSLESKSFEFKDIVKIGRTHTQDATPLTLGQEFGGYATQVEYGLNRVACTLPR 180

Query: 252 MYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAAHDAFVETSGAL 311
           +YQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETN PFVTAENKFEALAAHDA VETSG+L
Sbjct: 181 IYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDACVETSGSL 240

Query: 312 NTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALTMVCAQVM 371
           NT+ATSLMKIANDIR LGSGPRCGLGEL LPENEPGSSIMPGKVNPTQCEALTMVCAQVM
Sbjct: 241 NTIATSLMKIANDIRFLGSGPRCGLGELSLPENEPGSSIMPGKVNPTQCEALTMVCAQVM 300

Query: 372 GNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRGIQANTERISKL 431
           GNHVAVT+GGSNGHFELNVFKP+IA+ALLHS+RL+ DASASFEKNCVRGI+AN ERISKL
Sbjct: 301 GNHVAVTIGGSNGHFELNVFKPVIASALLHSIRLIADASASFEKNCVRGIEANRERISKL 360

Query: 432 LREVSFMLIFI----IGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEFDKHVVPEKMI 491
           L E S ML+      IGYDNAAAVAK AHKEG TLK AA+KLGVL+ EEFD  VVPEKMI
Sbjct: 361 LHE-SLMLVTSLNPKIGYDNAAAVAKRAHKEGCTLKHAAMKLGVLTSEEFDTLVVPEKMI 420

The following BLAST results are available for this feature:

BLAST of MS019016 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022155265.1	1.1e-272	97.98	fumarate hydratase 1, mitochondrial [Momordica charantia]	[more]
XP_022984048.1	1.1e-264	95.15	fumarate hydratase 1, mitochondrial-like isoform X2 [Cucurbita maxima]	[more]
XP_023528894.1	1.3e-263	94.75	fumarate hydratase 1, mitochondrial-like [Cucurbita pepo subsp. pepo]	[more]
KAA0044396.1	1.1e-262	94.33	fumarate hydratase 1 [Cucumis melo var. makuwa]	[more]
XP_004152237.1	1.4e-262	94.14	fumarate hydratase 1, mitochondrial [Cucumis sativus] >KGN52832.1 hypothetical p...	[more]

BLAST of MS019016 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P93033	1.7e-242	87.78	Fumarate hydratase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FUM1 PE=1...	[more]
Q9FI53	2.7e-237	88.68	Fumarate hydratase 2 OS=Arabidopsis thaliana OX=3702 GN=FUM2 PE=1 SV=1	[more]
P14408	2.4e-185	69.68	Fumarate hydratase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Fh PE=1 SV=2	[more]
Q60HF9	4.1e-185	69.79	Fumarate hydratase, mitochondrial OS=Macaca fascicularis OX=9541 GN=FH PE=2 SV=1	[more]
P07954	7.1e-185	69.79	Fumarate hydratase, mitochondrial OS=Homo sapiens OX=9606 GN=FH PE=1 SV=3	[more]

BLAST of MS019016 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DLZ0	5.5e-273	97.98	Fumarate hydratase OS=Momordica charantia OX=3673 GN=LOC111022407 PE=3 SV=1	[more]
A0A6J1J9G0	5.5e-265	95.15	Fumarate hydratase OS=Cucurbita maxima OX=3661 GN=LOC111482474 PE=3 SV=1	[more]
A0A5A7TQL4	5.1e-263	94.33	Fumarate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G00...	[more]
A0A0A0KWM0	6.7e-263	94.14	Fumarate hydratase OS=Cucumis sativus OX=3659 GN=Csa_4G001990 PE=3 SV=1	[more]
A0A5D3E117	8.8e-263	94.33	Fumarate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G0...	[more]

BLAST of MS019016 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G47510.1	1.2e-243	87.78	fumarase 1	[more]
AT2G47510.2	1.2e-243	87.78	fumarase 1	[more]
AT5G50950.2	1.9e-238	88.68	FUMARASE 2	[more]
AT5G50950.1	2.2e-226	87.06	FUMARASE 2	[more]
AT5G50950.3	8.2e-213	88.21	FUMARASE 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000362	Fumarate lyase family	PRINTS	PR00149	FUMRATELYASE	coord: 164..182 score: 38.81 coord: 347..363 score: 66.75 coord: 301..328 score: 38.17 coord: 210..228 score: 55.26
IPR022761	Fumarate lyase, N-terminal	PFAM	PF00206	Lyase_1	coord: 42..372 e-value: 5.5E-117 score: 390.6
None	No IPR available	GENE3D	1.10.40.30		coord: 438..491 e-value: 4.4E-21 score: 76.8
None	No IPR available	GENE3D	1.20.200.10	Fumarase/aspartase (Central domain)	coord: 170..436 e-value: 7.1E-107 score: 358.7
None	No IPR available	PANTHER	PTHR11444:SF24	FUMARATE HYDRATASE, MITOCHONDRIAL	coord: 16..491
IPR018951	Fumarase C, C-terminal	PFAM	PF10415	FumaraseC_C	coord: 442..488 e-value: 2.4E-18 score: 66.3
IPR005677	Fumarate hydratase, class II	TIGRFAM	TIGR00979	TIGR00979	coord: 34..488 e-value: 1.5E-219 score: 727.4
IPR005677	Fumarate hydratase, class II	PANTHER	PTHR11444	ASPARTATEAMMONIA/ARGININOSUCCINATE/ADENYLOSUCCINATE LYASE	coord: 16..491
IPR005677	Fumarate hydratase, class II	HAMAP	MF_00743	FumaraseC	coord: 34..489 score: 85.206932
IPR005677	Fumarate hydratase, class II	CDD	cd01362	Fumarase_classII	coord: 35..486 e-value: 0.0 score: 862.192
IPR024083	Fumarase/histidase, N-terminal	GENE3D	1.10.275.10		coord: 35..169 e-value: 2.6E-63 score: 214.3
IPR020557	Fumarate lyase, conserved site	PROSITE	PS00163	FUMARATE_LYASES	coord: 347..356
IPR008948	L-Aspartase-like	SUPERFAMILY	48557	L-aspartase-like	coord: 34..487

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS019016.1	MS019016.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006106	fumarate metabolic process
biological_process	GO:0006099	tricarboxylic acid cycle
cellular_component	GO:0045239	tricarboxylic acid cycle enzyme complex
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0004333	fumarate hydratase activity
molecular_function	GO:0016829	lyase activity